Services

Date

Field

Changed From

Changed To

Preferred Container Type

24 hour urine

24-hour urine - ACID bottle

Collection & Request Instructions

24-hour urine collection in plain bottle

Keep refrigerated during collection and send to laboratory immediately on completion

Patient Collection Instructions

24-hour urine collection in acid bottle

Keep refrigerated during collection and send to laboratory immediately on completion

Patient Collection Instructions

Method

24 hour urine, plain bottle. A 50 mL aliquot is sent with the recorded total 24 hrs volume.

An extra 50 mL aliquot needs to be stored.

24 hour urine, acid bottle. A 50 mL aliquot is sent with the recorded total 24 hrs volume.

An extra 50 mL aliquot needs to be stored.

Date

Field

Changed From

Changed To

Collection & Request Instructions

Clinical notes must be completed

If pregnant female, please state pregancy in weeks

AFP is useful in monitoring some testicular germ cell tumours and primary hepatocelluar carcnioma. After treatment, eg. orchidectomy, levels fall with a half-life of approximately 45 days. Values > 500 mg/L are suggestive of malignancy

However 50% of all malignancies have values < 500 mg/L. AFP can also be raised in a variety of non-malignant liver disorders, where levels are usually < 100 mg/L.

Clinical notes must be completed

If pregnant female, please state pregancy in weeks

AFP is useful in monitoring some testicular germ cell tumours and primary hepatocelluar carcnioma. After treatment, eg. orchidectomy, levels fall with a half-life of approximately 45 days. Values > 500 ug/L are suggestive of malignancy

However 50% of all malignancies have values < 500 ug/L. AFP can also be raised in a variety of non-malignant liver disorders, where levels are usually < 100 ug/L.

Date

Field

Changed From

Changed To

Frequency

Monthly

Date

Field

Changed From

Changed To

Collection & Request Instructions

LAB STAFF NOTE : The Reference Lab will only receive aliquots which are sent in a metal-free aliquot tube (see container picture above).  Sample must be decanted by pour-off (do not use plastic pipettes). 

LAB STAFF NOTE : The Reference Lab will only receive aliquots which are sent in a metal-free aliquot tube (see container picture above).  Sample must be decanted by pour-off (do not use plastic pipettes). 

Collection & Request Instructions

The Reference Lab will only receive aliquots which are sent in a metal-free aliquot tube (see container picture above).  Sample must be decanted by pour-off (do not use plastic pipettes). 

LAB STAFF NOTE : The Reference Lab will only receive aliquots which are sent in a metal-free aliquot tube (see container picture above).  Sample must be decanted by pour-off (do not use plastic pipettes). 

Preferred Container Type

Trace metal tube ( Dark blue )

Trace Metal with Aliquot

Collection & Request Instructions

Do Not spin - Transport at 4⁰C

The Reference Lab will only receive aliquots which are sent in a metal-free aliquot tube (see container picture above).  Sample must be decanted by pour-off (do not use plastic pipettes). 

Date

Field

Changed From

Changed To

Collection & Request Instructions

Pre-dose serum is required.

Trough level - collect at least 6-8 hours after last dose.

Trough level - collect at least 6-8 hours after last dose.

Processing Instructions

Desethylamiodarone is the major metabolite of Amiodarone

Spin, aliquot and freeze plasma.

External Laboratory

St Vincents Hospital

Royal Brisbane & Womens Hospital

Date

Field

Changed From

Changed To

Collection & Request Instructions

Transport ON ICE IMMEDIATELY to lab

Collection & Request Instructions

Preferable to receive a fasting sample.

Transport to laboratory on ice or transport immediately to laboratory and contact the laboratory staff to expect the sample.

Date

Field

Changed From

Changed To

Alternative names

Mitochondrial Antibodies

Mitochondrial Antibodies, AMA

Date

Field

Changed From

Changed To

Volume (Adults)

1.0 mL

Minimum/Paediatric Volume

0.2 mL

Collection & Request Instructions

Wicks and Gauze will not be accepted.

Notes

The presence of asialotransferrin in a fluid indicates that it is, or contains, CSF.

The presence of asialotransferrin in a fluid indicates that it is, or contains, CSF.

Pathologist approval required.

Date

Field

Changed From

Changed To

Notes

Refer to Objectify Poliy # 2086. Collection of Blood Cultures

 Refer to Objectify Poliy # 2086. Collection of Blood Cultures

Notes

Blood Culture Collection Instructions

Blood Culture Clinical Information

Refer to Objectify Poliy # 2086. Collection of Blood Cultures

Date

Field

Changed From

Changed To

Notes

Blood Culture Collection Instructions

Blood Culture Clinical Information

 Refer to Objectify Policy # 2086 Collection of Blood Cultures

Date

Field

Changed From

Changed To

Collection & Request Instructions

Bont marrow trephine requires at least 4 hours fixation prior to processing. Please consider this for an Urgent trephine result.

Bone Marrow Collection Instructions

Bont marrow trephine requires at least 4 hours fixation prior to processing. Please consider this for an Urgent trephine result.

Date

Field

Changed From

Changed To

Laboratory

Blood Bank

Referred Test

Processing Instructions

CRP-hs is run on a weekly basis in the Box Hill lab.  Specimens should be frozen at -20 within 24 hours of collection.

External Laboratory

St Vincents Hospital

External Transport Instructions

If transporting within 24 hours of collection, 4 deg transport is acceptable.  Otherwise, aliquot should be frozen.

Frequency

Weekly

Daily

Date

Field

Changed From

Changed To

External Laboratory

Neurogenetics Unit, Level 2, Royal Perth Hospital

Pathwest Lab Med WA

Frequency

As required

Method

Packed by certified personnel

Dr M Davis, Neurogenetics Unit, DPT of Anatomical Pathology, Level 2, North Block, RPA, Wellington Street, PERTH, WA 6000

(Ph) 08-92243136.

Packed by certified personnel

Dr M Davis, Diagnostic Genomics, Level 2

C/O Specimen Reception Ground Floor PP Building

QEII Medical Centre Hospital Avenue

Nedlands WA 6009 AUSTRALIA

(Ph) 08-63834243

Notes

Defects in NOTCH3 are the cause of cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL) . CADASIL causes a type of stroke and dementia of which key features include recurrent subcortical ischemic events and vascular dementia. The disorder affects relatively young adults of both sexes. Mutations affect highly conserved cysteine residues within epidermal growth factor (EGF)-like repeat domains in the extracellular part of the receptor.

Defects in NOTCH3 are the cause of cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL) . CADASIL causes a type of stroke and dementia of which key features include recurrent subcortical ischemic events and vascular dementia. The disorder affects relatively young adults of both sexes. Mutations affect highly conserved cysteine residues within epidermal growth factor (EGF)-like repeat domains in the extracellular part of the receptor.

Date

Field

Changed From

Changed To

Preferred Container Type

Trace Metal with Aliquot

Trace metal tube ( Dark blue )

Notes

Transport specimen at Room Temperature to lab
Sample haemolysis may make this test unreportable

LAB STAFF NOTE : The Reference Lab will only receive aliquots which are sent in a metal-free aliquot tube (see container picture above).  Sample must be decanted by pour-off (do not use plastic pipettes). 

Transport specimen at Room Temperature to lab
Sample haemolysis may make this test unreportable

Date

Field

Changed From

Changed To

Alternative names

Candida screen

Candida screen, Candida auris

Date

Field

Changed From

Changed To

Collection & Request Instructions

Please note : to avoid sample deterioration, DO NOT send CSF specimens via the vacuum chute system.

Depending on the tests requested, 3-4 separate tubes will need to be provided.  Note that the tubes are numbered so that order of collection can be identified – this order should be carefully followed, so that the optimal sample can be used for the requested tests.  It is also important to consider the tests that are required, as this will determine how much CSF is needed in each tube.  See the matrix below for details.

TUBE 1 is used for Cytology.

TUBE 2 is used for Glucose, Protein and Flow Cytometry.

(centrifuged in the lab – the supernatant is used for Biochemistry analysis (glucose, protein) and the cellular pellet will be used for flow cytometry if required).

TUBE 3 is for Microbiological analysis (cell count, Gram stain and culture) and any further infectious testing (e.g. viral PCR, mycobacterial PCR and culture, cryptococcal antigen).

TUBE 4 will be required only for;

         (a) Creutzfeld-Jacob Disease (CJD) testing OR

(b) Xanthochromia analysis (wrap the tube in foil to prevent    sample deterioration due to light).

Note, the ability to add further tests to a previously collected sample is dependent upon the volume provided.  Also, many tests (flow cytometry, cytology) can only be performed on fresh specimens.

Other specialised testing (e.g. oligoclonal bands) will be performed using the supplied samples.

Xanthochromia and CJD analysis cannot be performed on a blood-stained sample.

Please note : to avoid sample deterioration, DO NOT send CSF specimens via the vacuum chute system.

Depending on the tests requested, 3-4 separate tubes will need to be provided.  Note that the tubes are numbered so that order of collection can be identified – this order should be carefully followed, so that the optimal sample can be used for the requested tests.  It is also important to consider the tests that are required, as this will determine how much CSF is needed in each tube.  See the matrix below for details.

TUBE 1 is used for Cytology.

TUBE 2 is used for Glucose, Protein and Flow Cytometry.

(centrifuged in the lab – the supernatant is used for Biochemistry analysis (glucose, protein) and the cellular pellet will be used for flow cytometry if required).

TUBE 3 is for Microbiological analysis (cell count, Gram stain and culture) and any further infectious testing (e.g. viral PCR, mycobacterial PCR and culture, cryptococcal antigen).

TUBE 4 will be required only for;

         (a) Creutzfeld-Jacob Disease (CJD) testing OR

(b) Xanthochromia analysis (wrap the tube in foil to prevent    sample deterioration due to light).

Note, the ability to add further tests to a previously collected sample is dependent upon the volume provided.  Also, many tests (flow cytometry, cytology) can only be performed on fresh specimens.

Other specialised testing (e.g. oligoclonal bands) will be performed using the supplied samples.

Xanthochromia and CJD analysis cannot be performed on a blood-stained sample.

CSF Information - Laboratory Processing

Collection & Request Instructions

Please note : to avoid sample deterioration, DO NOT send CSF specimens via the vacuum chute system.

If additional tests required eg. CSF oligoclonal bands, a minimum of 1.0 ml is required.

Collect CSF into three separate, sterile, screw-capped tubes provided (labelled 1,2 & 3)

Tube 1 is for Biochemistry (for Protein and Glucose) 0.5ml (10 drops) required 

Tube 2 for Cytology 1.0ml (20 drops)  and/or Flow Cytometry (6ml)

For Flow Cytometry a minimum of 6ml is required  in Tube 2

For Flow Cytometry and Cytology a minimum of 7ml is required in Tube 2     

Tube 3 for Microbiology upto 10ml (Full tube)

If querying CJD the laboratory requires a 4th unopened tube containing at least 1ml of non-bloodstained CSF.

Xanthochromia investigation requires a 4th tube containing 1ml of CSF .

Please note : to avoid sample deterioration, DO NOT send CSF specimens via the vacuum chute system.

Depending on the tests requested, 3-4 separate tubes will need to be provided.  Note that the tubes are numbered so that order of collection can be identified – this order should be carefully followed, so that the optimal sample can be used for the requested tests.  It is also important to consider the tests that are required, as this will determine how much CSF is needed in each tube.  See the matrix below for details.

TUBE 1 is used for Cytology.

TUBE 2 is used for Glucose, Protein and Flow Cytometry.

(centrifuged in the lab – the supernatant is used for Biochemistry analysis (glucose, protein) and the cellular pellet will be used for flow cytometry if required).

TUBE 3 is for Microbiological analysis (cell count, Gram stain and culture) and any further infectious testing (e.g. viral PCR, mycobacterial PCR and culture, cryptococcal antigen).

TUBE 4 will be required only for;

         (a) Creutzfeld-Jacob Disease (CJD) testing OR

(b) Xanthochromia analysis (wrap the tube in foil to prevent    sample deterioration due to light).

Note, the ability to add further tests to a previously collected sample is dependent upon the volume provided.  Also, many tests (flow cytometry, cytology) can only be performed on fresh specimens.

Other specialised testing (e.g. oligoclonal bands) will be performed using the supplied samples.

Xanthochromia and CJD analysis cannot be performed on a blood-stained sample.

Date

Field

Changed From

Changed To

Collection & Request Instructions

The sample with the least amount of blood is prefered for Cytology.

Collect CSF into three separate, sterile, screw-capped tubes provided (labelled 1,2 & 3)

Tube 1 is for Biochemistry (for Protein and Glucose) 0.5ml (10 drops) required

Tube 2 for Cytology 1.0ml (20 drops)  and/or Flow Cytometry (6ml)

For Flow Cytometry a minimum of 6ml is required  in Tube 2

For Flow Cytometry and Cytology a minimum of 7ml is required in Tube 2    

Tube 3 for Microbiology upto 10ml (Full tube)

Xanthochromia investigation requires a 4th tube containing 1ml of CSF.

The sample with the least amount of blood is prefered for Cytology.

CSF SPECIMEN INFORMATION – LABORATORY PROCESSING

Depending on the tests requested, 3-4 separate tubes will need to be provided.  Note that the tubes are numbered so that order of collection can be identified – this order should be carefully followed, so that the optimal sample can be used for the requested tests.  It is also important to consider the tests that are required, as this will determine how much CSF is needed in each tube.  See the matrix below for details.

TUBE 1 is used for Cytology.

TUBE 2 is used for Glucose, Protein and Flow Cytometry.

(centrifuged in the lab – the supernatant is used for Biochemistry analysis (glucose, protein) and the cellular pellet will be used for flow cytometry if required).

TUBE 3 is for Microbiological analysis (cell count, Gram stain and culture) and any further infectious testing (e.g. viral PCR, mycobacterial PCR and culture, cryptococcal antigen).

TUBE 4 will be required only for;

         (a) Creutzfeld-Jacob Disease (CJD) testing OR

(b) Xanthochromia analysis (wrap the tube in foil to prevent    sample deterioration due to light).

Note, the ability to add further tests to a previously collected sample is dependent upon the volume provided.  Also, many tests (flow cytometry, cytology) can only be performed on fresh specimens.

Other specialised testing (e.g. oligoclonal bands) will be performed using the supplied samples.

Xanthochromia and CJD analysis cannot be performed on a blood-stained sample.

Processing Instructions

Must be stored in the fridge.

Must be stored in the fridge.

Out of hours CSF samples for routine testing can be stored in the AP refrigerator.

Notes

Out of hours CSF samples for routine testing can be stored in the AP refrigerator.

Notes

out of hours CSF samples for cytology can be stored in the refrigerator for routine testing

Out of hours CSF samples for routine testing can be stored in the AP refrigerator.

Out of hours CSF samples for routine testing can be stored in the AP refrigerator.

Collection & Request Instructions

CSF must be delivered to laboratory immediately; cellular degeneration occurs within 30 minutes.

The sample with the least amount of blood is prefered for Cytology.

Collect CSF into three separate, sterile, screw-capped tubes provided (labelled 1,2 & 3)

Tube 1 is for Biochemistry (for Protein and Glucose) 0.5ml (10 drops) required

Tube 2 for Cytology 1.0ml (20 drops)  and/or Flow Cytometry (6ml)

For Flow Cytometry a minimum of 6ml is required  in Tube 2

For Flow Cytometry and Cytology a minimum of 7ml is required in Tube 2    

Tube 3 for Microbiology upto 10ml (Full tube)

Xanthochromia investigation requires a 4th tube containing 1ml of CSF .

The sample with the least amount of blood is prefered for Cytology.

Collect CSF into three separate, sterile, screw-capped tubes provided (labelled 1,2 & 3)

Tube 1 is for Biochemistry (for Protein and Glucose) 0.5ml (10 drops) required

Tube 2 for Cytology 1.0ml (20 drops)  and/or Flow Cytometry (6ml)

For Flow Cytometry a minimum of 6ml is required  in Tube 2

For Flow Cytometry and Cytology a minimum of 7ml is required in Tube 2    

Tube 3 for Microbiology upto 10ml (Full tube)

Xanthochromia investigation requires a 4th tube containing 1ml of CSF.

External Transport Instructions

All out of hours specimens from Anglss and Maroondah should be sent to Box Hill ASAP for processing.

After hours cytology CSF can be prepared by microbiology staff. In the event microbiology staff are not in attendance and will not be called in, please call the Anatomical Pathology Scientist on call.

After hours cytology CSF can be prepared by microbiology staff. In the event microbiology staff are not in attendance please store in the AP fridge.

Method

CSF sample processing:

If Cytology/Flow Cytometry requested  : Tube 1-Biochemistry, Tube 2- Cytology/Flow Cytometry, Tube 3-Microbiology

If Cytology/Flow Cytometry not requested: Tube 1 and 3-Microbiology, Tube 2-Biochemistry

Any confusion between the terms cell count and cytology could be cleared up when the cell count is rung through to the requesting doctor by the scientist. At this point it could be determined whether cytology is a genuine request or not (all to be documented in F8 notes). The CSF can then be forwarded expediently to BHH for cytospin if required.

CSF sample processing:

If Cytology/Flow Cytometry requested  : Tube 1-Biochemistry, Tube 2- Cytology/Flow Cytometry, Tube 3-Microbiology

If Cytology/Flow Cytometry not requested: Tube 1 and 3-Microbiology, Tube 2-Biochemistry

Any confusion between the terms cell count and cytology could be cleared up when the cell count is rung through to the requesting doctor by the scientist. At this point it could be determined whether cytology is a genuine request or not (all to be documented in F8 notes).

Notes

out of hours CSF samples for cytology can be stored in the refrigerator for routine testing

Out of hours CSF samples for routine testing can be stored in the AP refrigerator.

Date

Field

Changed From

Changed To

Notes

Special tests available on request:

PCR for viruses

Acid-fast stain and/or culture for Mycobacteria

Cryptococcal antigen

Serological test for Syphilis (must be paired with 5mL serum sample or test will not be performed)

Viral Serology, if CSF is accompanied by serum sample

Flow Cytometry - requires a 4th tube

The turnaround time for CSF microscopy are 30-40 minutes.

The turnaround time for CSF culture are 3-5 days.

CSF SPECIMEN INFORMATION – LABORATORY PROCESSING

Depending on the tests requested, 3-4 separate tubes will need to be provided.  Note that the tubes are numbered so that order of collection can be identified – this order should be carefully followed, so that the optimal sample can be used for the requested tests.  It is also important to consider the tests that are required, as this will determine how much CSF is needed in each tube.  See the matrix below for details.

TUBE 1 is used for Cytology.

TUBE 2 is used for Glucose, Protein and Flow Cytometry.

(centrifuged in the lab – the supernatant is used for Biochemistry analysis (glucose, protein) and the cellular pellet will be used for flow cytometry if required).

TUBE 3 is for Microbiological analysis (cell count, Gram stain and culture) and any further infectious testing (e.g. viral PCR, mycobacterial PCR and culture, cryptococcal antigen).

TUBE 4 will be required only for;

         (a) Creutzfeld-Jacob Disease (CJD) testing OR

(b) Xanthochromia analysis (wrap the tube in foil to prevent    sample deterioration due to light).

Note, the ability to add further tests to a previously collected sample is dependent upon the volume provided.  Also, many tests (flow cytometry, cytology) can only be performed on fresh specimens.

Other specialised testing (e.g. oligoclonal bands) will be performed using the supplied samples.

Xanthochromia and CJD analysis cannot be performed on a blood-stained sample.

Special tests available on request:

PCR for viruses

Acid-fast stain and/or culture for Mycobacteria

Cryptococcal antigen

Serological test for Syphilis (must be paired with 5mL serum sample or test will not be performed)

Viral Serology, if CSF is accompanied by serum sample

Flow Cytometry - requires a 4th tube

The turnaround time for CSF microscopy are 30-40 minutes.

The turnaround time for CSF culture are 3-5 days.

Date

Field

Changed From

Changed To

Notes

Collect CSF into three separate, sterile, screw-capped tubes provided (labelled 1,2 & 3)

Tube 1 is for Biochemistry (for Protein and Glucose) 0.5ml (10 drops) required 

Tube 2 for Cytology 1.0ml (20 drops)  and/or Flow Cytometry (6ml)

For Flow Cytometry a minimum of 6ml is required  in Tube 2

For Flow Cytometry and Cytology a minimum of 7ml is required in Tube 2     

Tube 3 for Microbiology upto 10ml (Full tube)

If querying CJD the laboratory requires a 4th unopened tube containing at least 1ml of non-bloodstained CSF.

Xanthochromia investigation requires a 4th tube containing 1ml of CSF .

Cell Reference Ranges

                  Adults:

                  0 - 5 lymphocytes (x10*6/L)

                  0  neutrophils

                   Neonates:

                  (£ 1 month)

                  0 – 20 white cells (x10*6/L, predominantly lymphocytes)

                  0 - several hundred erythrocytes

Leucocyte:erythrocyte (WBC:RBC) ratio is normally 1:500 (including traumatic tap).

CSF SPECIMEN INFORMATION – LABORATORY PROCESSING

Depending on the tests requested, 3-4 separate tubes will need to be provided.  Note that the tubes are numbered so that order of collection can be identified – this order should be carefully followed, so that the optimal sample can be used for the requested tests.  It is also important to consider the tests that are required, as this will determine how much CSF is needed in each tube.  See the matrix below for details.

TUBE 1 is used for Cytology.

TUBE 2 is used for Glucose, Protein and Flow Cytometry.

(centrifuged in the lab – the supernatant is used for Biochemistry analysis (glucose, protein) and the cellular pellet will be used for flow cytometry if required).

TUBE 3 is for Microbiological analysis (cell count, Gram stain and culture) and any further infectious testing (e.g. viral PCR, mycobacterial PCR and culture, cryptococcal antigen).

TUBE 4 will be required only for;

         (a) Creutzfeld-Jacob Disease (CJD) testing OR

(b) Xanthochromia analysis (wrap the tube in foil to prevent    sample deterioration due to light).

Note, the ability to add further tests to a previously collected sample is dependent upon the volume provided.  Also, many tests (flow cytometry, cytology) can only be performed on fresh specimens.

Other specialised testing (e.g. oligoclonal bands) will be performed using the supplied samples.

Xanthochromia and CJD analysis cannot be performed on a blood-stained sample.

Date

Field

Changed From

Changed To

Preferred Container Type

Trace metal tube ( Dark blue )

Trace Metal with Aliquot

Collection & Request Instructions

LAB STAFF NOTE : The Reference Lab will only receive aliquots which are sent in a metal-free aliquot tube (see container picture above).  Sample must be decanted by pour-off (do not use plastic pipettes). 

Date

Field

Changed From

Changed To

Notes

When blood is collected from an arterial line it is necessary to discard the first 20 mL of blood to clear any heparin from the line before collecting the blood for coagulation tests

When collection is by vacuum tube, the coagulation tube should be collected second to avoid artefactual shortening of coagulation assays due to trauma of venesection

Whe blood is collected for an APTT or Anti-Xa Assay for control of heparin or low molecular weight heparin therapy, it is essential that the citrate tube is sent immediately to the laboratory

Tests include : INR, APTT

Do not use 1 ml citrate tubes.

When blood is collected from an arterial line it is necessary to discard the first 20 mL of blood to clear any heparin from the line before collecting the blood for coagulation tests

When collection is by vacuum tube, the coagulation tube should be collected second to avoid artefactual shortening of coagulation assays due to trauma of venesection

Whe blood is collected for an APTT or Anti-Xa Assay for control of heparin or low molecular weight heparin therapy, it is essential that the citrate tube is sent immediately to the laboratory

Do not use 1 ml citrate tubes.

Request Group

COAG

COAGP

Date

Field

Changed From

Changed To

Alternative names

extended panel PCR, MERS PCR, novel coronavirus PCR, Respiratory Virus NAD, SARS PCR

extended panel PCR, MERS PCR, novel coronavirus PCR, Respiratory Virus NAD, SARS PCR, novel Wuhan

Alternative names

Respiratory Virus NAD, SARS PCR

Respiratory Virus NAD, SARS PCR, extended panel PCR, novel coronavirus PCR, MERS PCR

Request Group

RESPCR

RESPX

Date

Field

Changed From

Changed To

External Laboratory

Victorian Infectious Diseases Reference Laboratory (V.I.D.R.L)

Collection & Request Instructions

Nasopharyngeal swab in RED Copan viral transport medium

Nasal/oropharyngeal swab in GREEN Kang Jian viral transport medium

Nasal/oropharyngeal swab in GREEN Kang Jian viral transport medium

Paediatric - Nasopharyngeal swab in RED Copan viral transport medium

Notes

For Rapid testing from Emergency Department - swab must be accompanied by signed Rapid Test Authorisation Form.

DO NOT call the laboratory for results/enquiries. Inpatient tests will be prioritised and results available on EMR ASAP.

Sputum samples are referred to an external laboratory - results will be delayed.

Samples MUST be in a clear specimen bag within an outer GREEN specimen bag

DO NOT send samples in the Pathology Chute

For Rapid testing from Emergency Department - swab must be accompanied by signed Rapid Test Authorisation Form.

DO NOT call the laboratory for results/enquiries. Inpatient tests will be prioritised and results available on EMR ASAP.

Sputum samples are referred to an external laboratory - results will be delayed.

Preferred Specimen Type

Nasopharyngeal swab
NTS - nose & throat swab

Nasopharyngeal swab
NTS - nose & throat swab
Sputum

Preferred Container Type

Viral Transport Media - Nasopharyngeal
respiratory PCR requirements
See Instructions

Viral Transport Media - Nasopharyngeal
See Instructions
Sterile Jar

Notes

For Rapid testing from Emergency Department - swab must be accompanied by signed Rapid Test Authorisation Form.

DO NOT call the laboratory for results/enquiries. Inpatient tests will be prioritised and results available on EMR.

For Rapid testing from Emergency Department - swab must be accompanied by signed Rapid Test Authorisation Form.

DO NOT call the laboratory for results/enquiries. Inpatient tests will be prioritised and results available on EMR ASAP.

Sputum samples are referred to an external laboratory - results will be delayed.

Date

Field

Changed From

Changed To

Method

NOTIFY REGISTRY THAT SAMPLE IS BEING SENT.

Transport on dry ice.

CSF : Test is run weekly. Samples received by 4 p.m. Friday will have the result issued by the end of the following week.

 Blood : Test for PRNP (Prion protein gene)

5 mLs in EDTA tube

Send to

The Australian National CJD Registry

Dept. of Pathology, Faculty of Medicine

The University of Melbourne

Melbourne Brain Centre (Kenneth Myer Building)

Cnr Genetics Lane & Royal Pde

Gate 11, Rear Loading Dock

Parkville , Victoria 3052

Phone 8344 1949

Fax 8349 5105

Link to request form Updated 14.6.18

https://www.florey.edu.au/uploads/content/CJD-FO-008_CSF_Specimen_Data_Sheet_with_14-3-3_and_Tau.pdf

NOTIFY REGISTRY THAT SAMPLE IS BEING SENT.

Transport on dry ice.

CSF : Test is run weekly. Samples received by 4 p.m. Friday will have the result issued by the end of the following week.

 Blood : Test for PRNP (Prion protein gene)

5 mLs in EDTA tube

Send to

The Australian National CJD Registry

Dept. of Pathology, Faculty of Medicine

The University of Melbourne

Melbourne Brain Centre (Kenneth Myer Building)

Cnr Genetics Lane & Royal Pde

Gate 11, Rear Loading Dock

Parkville , Victoria 3052

Phone 8344 1949

Fax 8349 5105

Date

Field

Changed From

Changed To

Method

PLEASE NOTE : CYCLOSPORIN REFERRALS MUST ONLY BE SENT TO THE AUSTIN - THEY MUSY NOT BE SENT TO THE ALFRED AS THEY USE A METHOD THAT DOES NOT CORRESPOND WITH THE EXPECTED REFEERNCE RANGES.

PLEASE NOTE : CYCLOSPORIN send to the Austin Biochemistry Department.

Collection & Request Instructions

In post transplant patients a 2 hour post dose is the preferred specimen, please indicate dosing information on the request slip.

Specimen must arrive at the Box Hill laboratory before 1200 Mon-Fri.

Separate EDTA specimen required.

In post transplant patients a 2 hour post dose is the preferred specimen, please indicate dosing information on the request slip.

Specimen must arrive at the Box Hill laboratory before 1200 everyday.

Separate EDTA specimen required.

Date

Field

Changed From

Changed To

Collection & Request Instructions

Newborn/Infants: 1-2 ml EDTA

Children: 2-5 ml EDTA

Adults: 5-10 ml EDTA

Newborn/Infants: 1-2 ml EDTA

Children: 2-5 ml EDTA

Adults: 5-10 ml EDTA

Frequency

As required

Date

Field

Changed From

Changed To

Assay name

Cytogenetics - Tumour (Adult)

Cytogenetics - Tumour Tissue Block (Adult)

Date

Field

Changed From

Changed To

Assay name

SKIN BIOPSY - KARYOTYPE

Cytogenetics Tissue Non-Malignant (SKIN BIOPSY - KARYOTYPE)

Preferred Specimen Type

Biopsy

Tissue

Optional Specimen Type

Biopsy

Collection & Request Instructions

Refrigerate, send to lab upon completion

Collection & Request Instructions

https://handbook.monashpathology.org/index?testid=1507

https://handbook.monashpathology.org/index?testid=1507

Collect aseptically. Collect x2 skin punches from extant patients.

Collect approx 5mm square (with 2-3mm depth) skin biopsy from deceased patients.

Use RPMI (obtain from Pathology) for transport. Do not allow the sample to dry out.

External Transport Instructions

Send to Anatomical Pathology for send out to MMC.

Notes

Send to Pathology for send out to MMC.

Date

Field

Changed From

Changed To

Processing Instructions

Place the biopsy specimen in the centre of a piece of Parafilm about 3cm square, and fold the Parafilm over the specimen so that it forms an envelope around the specimen. Then place this in a capped plastic tube with an appropriate label. Lab Notes Store and transport frozen.

THE TISSUE SAMPLE MUST BE FROZEN IMMEDIATELY AFTER COLLECTION, AS FOLLOWS.
1. WRAP (FOLD) THE TISSUE SAMPLE IN PARAFILM FIRST.
2. THEN WRAP THE SAMPLE IN ALFOIL.
3. FREEZE THE SPECIMEN ASAP.
4. SEND ON DRY ICE

External Laboratory

Royal Childrens Hospital

Sullivan Nicolaides Pathology ( Via Melb Path)

Date

Field

Changed From

Changed To

Collection & Request Instructions

Room temperature

This test is not covered by Medicare.

Cost for testing is $250

.

Room temperature

This test is not covered by Medicare.

Cost for testing is $300.

Processing Instructions

Interstate courier- Room Temperature

Department Of Molecular Genetics, Children's Hospital at Westmead, Loading Dock 5, Redbank Road, Northmead, NSW 2152.

For results Tel: 02-98453244

Interstate courier- Room Temperature

Department Of Molecular Genetics, Children's Hospital at Westmead, Loading Dock 5, Redbank Road, Northmead, NSW 2152.

For results Tel: 02-98453244

Date

Field

Changed From

Changed To

Preferred Container Type

Slide & carrier

See Instructions

Collection & Request Instructions

A request slip indicating the clinical notes, prefered FISH probe if know and tissue type.

The follwing 2 micron sections of tissue on positively charged slides (as per immunohistochemistry), clearly labelled with patient name and section thickness must be sent:

a. 4 patient slides per probe. NOTE: For MYC testing a minimum of 6 slides are required. For >2 probes eg. "double hit" samples a minimum of 9 slides are required.

b. 1 H&E stained slide of the section intended for FISH with a marker indicating the optimal region for FISH - ie. the region wiht the most concentrated area of tumour.

A request slip indicating the clinical notes, prefered FISH probe if known and tissue type.

The follwing 2 micron sections of tissue on positively charged slides (as per immunohistochemistry), clearly labelled with patient name and section thickness must be sent:

a. 4 patient slides per probe. NOTE: For MYC testing a minimum of 6 slides are required. For >2 probes eg. "double hit" samples a minimum of 9 slides are required.

b. 1 H&E stained slide of the section intended for FISH with a marker indicating the optimal region for FISH - ie. the region with the most concentrated area of tumour.

See attached referal instruction sheet: http://pathology.easternhealth.org.au/handbook/downloads/anatomical-pathology/FISH_VCGS_instructions_2018.pdf

External Transport Instructions

Please deliver all specimes to:

Victorian Cancer Cytogenetics Service

St Vincent's Hospital Melbourne

Second Floor, Inpatient Services Building

41 Victoria Parade, Fitzroy, VIC, 3065.

T: 9288-4154   F:9288-4155

lynda.campbell@svhm.org.au or fran.omalley@svhm.org.au

Please deliver all specimes to:

Victorian Cancer Cytogenetics Service

St Vincent's Hospital Melbourne

Second Floor, Building A Destination A26

41 Victoria Parade, Fitzroy, VIC, 3065.

T: 9231 4154   F:9231 4155

meaghan.wall@svha.org.au or fran.omalley@svhm.org.au

Date

Field

Changed From

Changed To

Processing Instructions

Frozen

Frozen

External Transport Instructions

The Sample must be separated and Frozen. fPSA is only stable for 24 hrs Max. The total PSA must be performed before the chemical pathologist/senior scientist can determine if the request is appropriate.

The Sample must be separated and Frozen. fPSA is only stable for 24 hrs Max. The total PSA must be performed before the chemical pathologist/senior scientist can determine if the request is appropriate.

Date

Field

Changed From

Changed To

Request Group

A1CS

HBA1CP, A1CP

Date

Field

Changed From

Changed To

Frequency

Daily

As required

Notes

• An FBE is usually performed concurrently
• The presence of Heinz bodies indicates oxidant drug haemolysis, asplenia or unstable haemoglobin

An FBE is usually performed concurrently

The presence of Heinz bodies indicates oxidant drug haemolysis, asplenia or unstable haemoglobin

Date

Field

Changed From

Changed To

Laboratory

Blood Bank

Referred Test

Collection & Request Instructions

HITScreen/ELISA testing is only available after consultation with the Consultant Haematologist or Haematology Registrar.

HITScreen/ELISA testing (HITE) is only available after consultation with the Consultant Haematologist or Haematology Registrar.

Collection & Request Instructions

Transport specimen at Room Temperature to lab

Collection & Request Instructions

The DiaMed Hits screen will detect approx. 85%of HITS positive patients.

If clinical indicators are strong but the DiaMed test negative, a serum sample can be forwarded for ELISA testing (HITE) at Monash Medical Centre

ELISA testing is only available after consultation with the Consultant Haematologist or Haematology Registrar.

ELISA testing is only available after consultation with the Consultant Haematologist or Haematology Registrar.

Processing Instructions

Laboratory staff at Monash Medical Centre must be informed if the ELISA test is to be done urgently.

transport - serum frozen to MMC unless test is to be done same day.

(MMC Coagulation: 9594 3489)

Laboratory staff at Monash Medical Centre must be informed if the ELISA test is to be done urgently.

Transport - serum frozen to MMC.

(MMC Special Haematology: 9594 3490)

External Laboratory

Monash Pathology

Notes

Plain tube with gel

Keep at room temperature and send to laboratory immediately.   DO NOT FREEZE

Plain tube with gel

Date

Field

Changed From

Changed To

Request Group

HEPDAB & HEPDAG

HEPDAB

Date

Field

Changed From

Changed To

Alternative names

TTR, FGA, APOA1, LYZ or Amyloidosis Gene

Volume (Adults)

Minimum/Paediatric Volume

Date

Field

Changed From

Changed To

Alternative names

3-Hydroxy-3-Methylglutaryl-coenzyme A Reductase), Anti-HMGCR, Autoantibodies to HMGCR

3-Hydroxy-3-Methylglutaryl-coenzyme A Reductase, Anti-HMGCR, Autoantibodies to HMGCR

Date

Field

Changed From

Changed To

Processing Instructions

HoloTC is only stable for 3 days at 2-8 C, hence if it is being stored at an Eastern Health Lab over the weekend, freeze and transport frozen.

External Transport Instructions

Please send sample at 4 degrees C. for weekend storage please freeze.

Frequency

Daily

Laboratory

Referred Test

Biochemistry

External Laboratory

Alfred Hospital

Date

Field

Changed From

Changed To

Laboratory

Referred Test

Biochemistry

External Laboratory

St Vincents Hospital

Date

Field

Changed From

Changed To

Request Group

IMAT

Processing Instructions

Spin,aliquot plasma and freeze.

External Transport Instructions

Frozen sample to be packed by IATA trained personal.

Date

Field

Changed From

Changed To

Request Group

ISOHGT

Processing Instructions

A Blood Group and Antibody screen should be performed by Eastern Health Pathology in conjunction with this request ( in order to check for other antibodies and allow reporting of isohaemagglutinin titre ). Blood samples should be held and transported at 4 deg C. Samples can be held ( at 4 deg C ) for up to 3 days before dispatch.

A Blood Group and Antibody screen should be performed by Eastern Health Pathology in conjunction with this request ( in order to check for other antibodies and allow reporting of isohaemagglutinin titre ). Blood samples should be held and transported at 4 deg C. Samples can be held ( at 4 deg C ) for up to 3 days before dispatch.

Method

Isohaemagglutinin titre may be requested in organ transplant patients as a base line test and at different times post transplant. The test refers to the titre of any of anti A and anti B in serum. Each individual produces different quantities of these antibodies. In Renal transplant patients ( given non ABO matched organs ) a rise from baseline isohaemagglutinin titre may prompt clinical intervention.

Isohaemagglutinin titre may be requested in organ transplant patients as a base line test and at different times post transplant. The test refers to the titre of any of anti A and anti B in serum. Each individual produces different quantities of these antibodies. In Renal transplant patients ( given non ABO matched organs ) a rise from baseline isohaemagglutinin titre may prompt clinical intervention.

Date

Field

Changed From

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Collection & Request Instructions

Sample is stable at Room Temperature for at least 24 Hours.

JAK2 is for differentiation of reactive VS clonal myeloproliferative disorders.

Collect a separate sample for this test.

This test is not Medicare rebatable. (Cost $44.00 incl GST)

Sample is stable at Room Temperature for at least 24 Hours.

JAK2 is for differentiation of reactive VS clonal myeloproliferative disorders.

Collect a separate sample for this test.

Date

Field

Changed From

Changed To

Preferred Specimen Type

Serum

Plasma

Preferred Container Type

Plain tube no gel

Lithium heparin no gel

Date

Field

Changed From

Changed To

Notes

Room temperature.

Please note this test id not covered by Medicare Rebate - see 'Ordering' above.

Room temperature.

Please note this test is not covered by Medicare Rebate - see 'Ordering' above.

Notes

Room temperature.

This test is not covered by Medicare, cost will be $432.00 as of March 2016.

Room temperature.

Please note this test id not covered by Medicare Rebate - see 'Ordering' above.

Assay name

White Cell Enzymes

Leucocyte Lysomal Enzyme Analysis

Alternative names

Lysosomal Enzymes

White Cell Enzymes

Volume (Adults)

4 mL

8 mL

Minimum/Paediatric Volume

6 mL

Notes

Room temperature.

This test is not covered by Medicare, cost will be $474.00 as of March 2016.

Room temperature.

This test is not covered by Medicare, cost will be $432.00 as of March 2016.

Date

Field

Changed From

Changed To

External Laboratory

Pacific Laboratory Medicine Services

Royal Brisbane & Womens Hospital

Frequency

Daily

As required

Date

Field

Changed From

Changed To

Alternative names

Anti-myelin associated glycoprotein antibodies

Request Group

MAGIFA

MAGAB

Frequency

Weekly

Method

Email Melb path before sending at  sendaways.department@mps.com.au to advise the samples are coming.

Email Melb path before sending at  sendaways.department@mps.com.au to advise the samples are coming.

Date

Field

Changed From

Changed To

Request Group

UHG24

UHG24/UHG

Collection & Request Instructions

Urine mercury concentration can be done on spot urine sample.

Patient Collection Instructions

Urine mercury concentration can be done on spot urine sample.(UHG)

Patient Collection Instructions

Request Group

HGCRU

UHG24

Volume (Adults)

50 mL

External Laboratory

Sydney South West Pathology Service(RPA)

Alfred Hospital

Frequency

Daily

Date

Field

Changed From

Changed To

Preferred Container Type

Swab - red top, plain swab, wooden shaft
Sterile Jar

Swab - red top, plain swab, wooden shaft
Swab - blue top (Amies transport media)
Swab - white top, plain swab, plastic shaft
UTM/Sterile pot

Date

Field

Changed From

Changed To

Preferred Container Type

Not required

Sterile Jar

Collection & Request Instructions

Please contact the laboratory in advance to ensure a muscle biopsy kit is readily available for collection.

Collection is as per kit instructions.

Note: This is an URGENT send out test.

Laboratory hours are 9am - 5pm, Monday - Friday.

Note: This is an URGENT send out test.

Laboratory hours are 9am - 5pm, Monday - Friday.

Processing Instructions

Call the Victorian Neuromuscular Laboratory for kits.

Contact: 9076 5448

Kits are to be stored in the fridge.

Prior to giving the kit to theatre staff, add 2mls of concentrated glutaraldehyde into 18mls of cacodylate buffer (red top container).

Notify the Victorian Neuromuscular Laboratory.

Contact: 9076 5448

External Transport Instructions

This is an URGENT send out test.

Place the specimen in an esky with ice cubes and dispatch immediately.

Method

Call taxi from the lab. Fill out cab voucher logbook.

Send to: Victorian neuromuscular Laboratory Service (VNLS), Alfred Pathology Service, Ground Floor, main Ward Block, Commercial Road, Melbourne VIC 3004.

Contact: 9076 3150

Send to: Victorian Neuromuscular Laboratory Service (VNLS), Alfred Pathology Service, Ground Floor, Main Ward Block, Commercial Road, Melbourne VIC 3004. Contact: 9076 3150

Date

Field

Changed From

Changed To

Preferred Container Type

Sterile Jar
Swab - white top, plain swab, plastic shaft

Sterile Jar
Swab - white top, plain swab, plastic shaft
Swab - blue top (Amies transport media)

Date

Field

Changed From

Changed To

External Laboratory

Pacific Laboratory Medicine Services

External Transport Instructions

Sample must be transported on Dry Ice and packed accoring to IATA regulations by certified personnel.

Lab Contact: 

PaLMS Endocrinology
RNSH

Cameron Wood ph 02 9926 4161

Notes

This test is not Medicare rebateable and must be

transported interstate frozen. The patient must not have

been taking calcium supplements or Vitamin D and must

have hypercalacemia with a suppressed PTH. Test must

be discussed with Chemical Pathologist or Senior Scientist. 

Date

Field

Changed From

Changed To

Volume (Adults)

3.5 mL

Minimum/Paediatric Volume

3.5 mL

Collection & Request Instructions

 PLEASE CONTACT THE LABORATORY BEFORE COLLECTING SPECIMENS FOR THIS TEST.  Samples must be analysed within 3-4 hours of collection.

Do not use 2 mL citrate tubes.

Transport to laboratory immediately. DO NOT transport in chute.

Non - BHH Collection: Must be transported at room temperature to the Box Hill Hospital laboratory within 2 hours.  Notify  laboratory before collection and record medication (including all painkillers.)

 PLEASE CONTACT THE LABORATORY BEFORE COLLECTING SPECIMENS FOR THIS TEST.  Samples must be analysed within 3-4 hours of collection.

Do not use 2 mL citrate tubes. Required 2x citrate tubes.

Transport to laboratory immediately. DO NOT transport in chute.

Non - BHH Collection: Must be transported at room temperature to the Box Hill Hospital laboratory within 2 hours.  Notify  laboratory before collection and record medication (including all painkillers.)

Processing Instructions

DO NOT centrifuge .

DO NOT centrifuge .

Notes

• Used in screening for von Willebrand's disease and aspirin therapy.

Used in screening for von Willebrand's disease and aspirin therapy.

Date

Field

Changed From

Changed To

External Laboratory

Austin Pathology

Monash Pathology

Date

Field

Changed From

Changed To

Assay name

Cholinesterase

Pseudocholinesterase

Collection & Request Instructions

Please request serum BCHE activity with the Genetic test.

Genetic testing sample : EDTA  whole blood 5mL minimum

Please request serum BCHE activity with the Genetic test.

Genetic testing sample : EDTA  whole blood 5mL minimum  and serum for BCHE activity.

Alternative names

Pseudocholinesterase

Pseudocholinesterase, Butyrylcholinesterase

Preferred Container Type

Pink EDTA (6mL)

Pink EDTA (6mL)
Serum tube with gel

Collection & Request Instructions

Genetic testing sample : EDTA  whole blood 5mL minimum

Please request serum BCHE activity with the Genetic test.

Genetic testing sample : EDTA  whole blood 5mL minimum

Processing Instructions

Genetic testing on unspun whole blood is performed by:

Molecular Genetics Department, Pathwest

QE II Medical centre,

Hospital Ave, Nedlands WA.  6009

Phone Lab Results 08 6383 4223  Spec Recepn. 08 6383 4956

Enzyme testing (serum) : RPA (Sydney) Samples can be frozen, Batched & sent weekly

Genetic testing and enzyme testing: please send samples together packed with ice pack.

Please send patient financial consent form. Cost of the test: $150.

External Laboratory

Pathwest Lab Med WA

Sydney South West Pathology Service(RPA)

Request Group

PCHOLS

Date

Field

Changed From

Changed To

Collection & Request Instructions

Pyruvate is very unstable and requires a special tube which is avalable from  the VCGS - Metabolic Laboratory Ph; 8341 6355. A fresh tube must be arranged for each request.

When the specimen is recieved the complete sample must be frozen immediately. Before sending the sample to VCGS, phone the laboatory to inform them it is coming.

Test not available.

For further information please contact chemical pathologist.

Processing Instructions

Write all the necessary collection details on the tube, do not add any other labels as the tube has been pre weighed. Place the pyruvate tube in a cup of ice water to cool for at least 5 mins before collection.

Draw 1 mL  ( 0.7 to 1.3 mL is acceptable ) of blood using a syringe and add this to the tube, mix well, the blood will turn chocolate brown.  TAKE CARE as the preservative in the tube is a corrosive acid. Ensure you are wearing protective gear and use safe practices. If the contents spill, wash the area well with water.

Place the specimen back into the ice water and transport immediately to the laboratory. Ensure a specimen for lactate is taken around the same time.

External Laboratory

Victorian Clinical Genetics Sevices (V.C.G.S) - Royal Childrens Hospital

Date

Field

Changed From

Changed To

Collection & Request Instructions

 PLEASE NOTE : Quantiferon specimens must now be collected using a dedicated Quantiferon Collection Pack containing four tubes (see image above)

Specimen must be tested within 10 hours of collection.

DO NOT refrigerate specimen.

Collect Monday - Friday before 10:00am.

Used to detect latent infection with M.tuberculosis, not for the diagnosis and management of active tuberculosis.

Note: Heparinised blood required, minimum 5 mL

 PLEASE NOTE : Quantiferon specimens must now be collected using a dedicated Quantiferon Collection Pack containing four tubes (see image above)

Specimen must be received at Box Hill by 16:00hrs Monday-Friday

DO NOT refrigerate specimen.

Used to detect latent infection with M.tuberculosis, not for the diagnosis and management of active tuberculosis.

Processing Instructions

If the specimen is collected at Maroondah or Angliss Hospitals, please contact the BH Microbiology Dept (9895 3475) before sending the samples.

Do not centrifuge, refrigerate or freeze specimen.

If the specimen is collected outside of Box Hill Hospital, please contact the BH Microbiology Dept (9895 3475) before sending the samples.

Do not centrifuge, refrigerate or freeze specimen.

External Transport Instructions

When packing for transport, the specimens MUST be packed in a separate transport esky at room temperature.

DO NOT put in an esky with cold packs.

FOR MICROBIOLOGY STAFF

QUANTIFERON GOLD PROCESSING

Quantiferon Gold specimens must be processed as below before sending to VIDRL

Note: Tubes must be incubated within 16 hours of collection.

At 12.00-12.30 mix the Quantiferon Gold tubes by inverting 10 times.

Incubate the tubes UPRIGHT in incubator M120.

Write time of incubation in F8 notes.

The next day at 8 am centrifuge the tubes for 15 mins at 3000g

(Tubes must be incubated for 16 to 24 hours.)

Check tubes for complete separation, respin if necessary.

The tubes are then ready to be packed and sent to VIDRL

Write the incubation time (from F8 notes) on the cover sheet.

NOTE : There is no change to collection times. Store & transport specimens at room temperature.

When packing for transport, the specimens MUST be packed in a separate transport esky at room temperature.

DO NOT put in an esky with cold packs.

FOR MICROBIOLOGY STAFF

Quantiferon Gold specimens must be processed as below before sending to VIDRL

Note:

Tubes must be incubated within 16 hours of collection (keep at room temperature until incubation);

Incubation at 37°C must be for 16-24 hours;

After incubation, tubes must be centrifuged within 3 days (keep at room temperature until centrifugation).

QUANTIFERON GOLD PROCESSING

Processing: to be performed by Microbiology staff

Remix all tubes immediately before incubation by inverting 10 times

Incubate at 37°C, O₂ (M138 incubator). There is a metal rack in the incubator to stand the tubes upright during incubation

For specimens received between 0800 hrs and 1600 hrs, incubate overnight.

For specimens received after 1600 hrs, hold at room temperature and incubate at 0800 hrs the next day (for 24 hours) and sent to VIDRL on the following day (i.e. two days later)

At 0800hr each day, remove specimens from incubator and centrifuge for 15 minutes at 3000 RCF (g). The laboratory assistant will do this on weekdays, Blood Culture Bench Scientist will do this on Saturday and Sunday

After centrifugation, send specimens to VIDRL with the 1230 hrs courier

Specimens that are received in the laboratory, greater than 10 hours after collection will not be tested (KIMMS).

Date

Field

Changed From

Changed To

Collection & Request Instructions

White top tube is the preferred sample.

0.2ml of blood required for each allergen.

Blood collection: still can be done after skin allergy testing.

White top tube is the preferred sample.0.2ml of blood required for each allergen.

Blood collection: still can be done after skin allergy testing.

Omega-5-Gliadin: serum send to Melbourne Pathology

IgG 96 Food Allergy test is not a RAST test  It is performed at  Australian Clinical Labs.This test is NOT rebatable and costs approx  $260.

Processing Instructions

IgG 96 Food Allergy test is not a RAST test  It is performed at  Australian Clinical Labs.This test is NOT rebatable and costs approx  $260.

Omega-5-Gliadin: send to Melborne Pathology

IgG 96 Food Allergy test is not a RAST test  It is performed at  Australian Clinical Labs.This test is NOT rebatable and costs approx  $260.

Alternative names

Allergy IgE Antibodies, Allergy testing, Specific IgE Testing For Allergens

Allergy IgE Antibodies, Allergy testing, Specific IgE Testing For Allergens. SIgE

Date

Field

Changed From

Changed To

Collection & Request Instructions

Collection Requirements for both or either tests

Collect into 4 mL EDTA tubes at Room Temperature. Do not collect on ice

If both Renin and Aldosterone are requested together, they can be collected into the same EDTA tube

In the laboratory, spin and separate the plasma immediately. Plasma is stable at Room Temperature if analysis is performed on the same day.

Renin must be frozen at -80 C. Transport plasma at room temperature until it can be place in -80 C Freezer.(Must be done within 3-4).

Add-on tests for Renin and Aldosterone should not be done on samples which have been previously collected or stored at 4 C.

Renin will not be collected at Healesville Hospital and Yarra Ranges Health Collection Rooms.

Collection Requirements for both or either tests

Collect into 4 mL EDTA tubes at Room Temperature. Do not collect on ice

If both Renin and Aldosterone are requested together, they can be collected into the same EDTA tube

In the laboratory, spin and separate the plasma immediately. Plasma is stable at Room Temperature if analysis is performed on the same day.

Renin must be frozen at -80 C. Transport plasma at room temperature (Maroondah and Angliss) until it can be place in -80 C Freezer.(Must be done within 3-4 hors).

Add-on tests for Renin and Aldosterone should not be done on samples which have been previously collected or stored at 4 C.

Renin will not be collected at Healesville Hospital and Yarra Ranges Health Collection Rooms.

Collection & Request Instructions

Collection Requirements for both or either tests

• Collect into 4 mL EDTA tubes at Room Temperature. Do not collect on ice
• If both Renin and Aldosterone are requested together, they can be collected into the same EDTA tube
• In the laboratory, spin and separate the plasma immediately. Plasma is stable at Room Temperature if analysis is performed on the same day
• If the specimen arrives on the weekend or after hours, or if the specimen is referred from an external laboratory, freeze the plasma
• EDTA plasma samples from external laboratories should be stored and transported frozen
• Whole blood or plasma stored at 4 Deg C is not suitable
• Add-on tests for Renin and Aldosterone should not be done on samples which have been previously collected or stored at 4 Deg C

Collection Requirements for both or either tests

Collect into 4 mL EDTA tubes at Room Temperature. Do not collect on ice

If both Renin and Aldosterone are requested together, they can be collected into the same EDTA tube

In the laboratory, spin and separate the plasma immediately. Plasma is stable at Room Temperature if analysis is performed on the same day.

Renin must be frozen at -80 C. Transport plasma at room temperature until it can be place in -80 C Freezer.(Must be done within 3-4).

Add-on tests for Renin and Aldosterone should not be done on samples which have been previously collected or stored at 4 C.

Renin will not be collected at Healesville Hospital and Yarra Ranges Health Collection Rooms.

Processing Instructions

Spin,seperate and freeze, transport frozen.

Minimum plasma volume: 500 ul (1mL if aldosterone is also requested)

Spin,seperate and freeze (-80 C), transport frozen.

Minimum plasma volume: 500 ul (1mL if aldosterone is also requested)

Notes

Prior to testing, patients should be withdrawn from diuretics, ACE inhibitors, angiotensin II receptor antagonists, beta blockers and calcium channel blockers for 3 weeks. Patients should also be well hydrated, have had 7g/day of salt for one week and have normal plasma potassium and bicarbonate levels.

•  Deliver to laboratory ASAP at room temperature

Prior to testing, patients should be withdrawn from diuretics, ACE inhibitors, angiotensin II receptor antagonists, beta blockers and calcium channel blockers for 3 weeks. Patients should also be well hydrated, have had 7g/day of salt for one week and have normal plasma potassium and bicarbonate levels.

 Deliver to laboratory ASAP at room temperature

Date

Field

Changed From

Changed To

Notes

This PCR will detect and identify: Influenza A virus, Influenza B virus and Respiratory syncytial virus (RSV).

Transport at room temperature to the Laboratory

Testing is performed at Box Hill, Angliss, and Maroondah.

Samples received after hours may have a longer TAT due to other urgent critical pathology testing occuring.

This PCR will detect and identify: Influenza A virus, Influenza B virus and Respiratory syncytial virus (RSV).

Transport at room temperature to the Laboratory

Testing is performed at Box Hill, Angliss, and Maroondah.

Samples received after hours may have a longer TAT due to other urgent critical pathology testing occurring.

Notes

This PCR will detect and identify: Influenza A virus, Influenza B virus and Respiratory syncytial virus (RSV).

Transport at room temperature to the Laboratory

Testing is performed at Box Hill, Angliss, and Maroondah.

Samples received after hours may have a longer TAT due to other urgent critical pathology testing occuring.

This PCR will detect and identify: Influenza A virus, Influenza B virus and Respiratory syncytial virus (RSV).

Transport at room temperature to the Laboratory

Testing is performed at Box Hill, Angliss, and Maroondah.

Samples received after hours may have a longer TAT due to other urgent critical pathology testing occuring.

Notes

This PCR will detect and identify: Influenza A virus, Influenza B virus and Respiratory syncytial virus (RSV).

Transport at room temperature to the Laboratory

Testing is performed at Box Hill, Angliss, and Maroondah.

TAT is a guide only. Minimal staffing levels after hours may mean a longer TAT due to other urgent pathology testing occuring (ie urgent Troponin, urgent transfusions etc).

This PCR will detect and identify: Influenza A virus, Influenza B virus and Respiratory syncytial virus (RSV).

Transport at room temperature to the Laboratory

Testing is performed at Box Hill, Angliss, and Maroondah.

Samples received after hours may have a longer TAT due to other urgent critical pathology testing occuring.

Method

All tests for respiratory virus PCR are considered urgent and must be delivered to the laboratory ASAP. Overnight specimens collected at BHH must be delivered to Microbiology by 7 a.m. Monday-Sunday.

If ICU/Oncology patients tested  Flu A/B and RSV negative on GeneXpert will be referred to Monash for respiratory virus panel. This includes Influenza A virus, Influenza B virus, Respiratory Syncitial Virus, Picornavirus (RV/EV), Parainfluenza viruses 1,2 and 3, Adenovirus, Human Metapneumovirus, Bordetella pertussis, Bordetella parapertussis, Mycoplasma pneumoniae, Legionella species, Chlamydiaceae.

The following specimens will be referred for testing-

Sputum, Bronchial Washings, Bronchoalveolar Lavages

Specimens for Respiratory Multiplex PCR will be referred to Monash Molecular Laboratory

Contact Monash Pathology 9594 4532 or if no answer try Bacteriology 9594 4565 as there is someone rostered 24/7

All tests for respiratory virus PCR are considered urgent and must be delivered to the laboratory ASAP.

ICU/Oncology patients testing negative for Flu A/B and RSV  will be referred to Monash for respiratory virus panel. This includes Influenza A virus, Influenza B virus, Respiratory Syncitial Virus, Picornavirus (RV/EV), Parainfluenza viruses 1,2 and 3, Adenovirus, Human Metapneumovirus, Bordetella pertussis, Bordetella parapertussis, Mycoplasma pneumoniae, Legionella species, Chlamydiaceae.

The following specimens will be referred for testing-

Sputum, Bronchial Washings, Bronchoalveolar Lavages

Specimens for Respiratory Multiplex PCR will be referred to Monash Molecular Laboratory

Contact Monash Pathology 9594 4532 or if no answer try Bacteriology 9594 4565 as there is someone rostered 24/7

Notes

This PCR will detect and identify: Influenza A virus, Influenza B virus and Respiratory syncytial virus (RSV).

Transport at room temperature to the Laboratory

Testing is performed at BHH and MH.

This PCR will detect and identify: Influenza A virus, Influenza B virus and Respiratory syncytial virus (RSV).

Transport at room temperature to the Laboratory

Testing is performed at Box Hill, Angliss, and Maroondah.

TAT is a guide only. Minimal staffing levels after hours may mean a longer TAT due to other urgent pathology testing occuring (ie urgent Troponin, urgent transfusions etc).

Date

Field

Changed From

Changed To

Processing Instructions

Free Testosterone/Calculated free testosterone requested: test code CFTEST

Also add Testosterone/SHBG/Albumin if free testosterone requested.

Date

Field

Changed From

Changed To

Collection & Request Instructions

Collect 2x4ml EDTA blood. DO NOT SPIN

Keep at 4 deg C, do not freeze.

The metabolites are stable for upto 4 days at 4 degress.

Collect 2x4ml EDTA blood. DO NOT SPIN

Keep at 4 deg C, do not freeze.

The metabolites are stable for upto 4 days at 4 degress.

Notes

Imuran is a trade name for azathioprine

The Thiopurine metabolites (6-TGN & 6-MMP) are used as a means of estimating the toxicity and efficacy of azathioprine treatment.

This test is performed by the Gastroenerology department. ( Phone No.9895 4632 )

The cost for the test is not covered by Medicare. Contact the testing lab for cost details.

Imuran is a trade name for azathioprine

The Thiopurine metabolites (6-TGN & 6-MMP) are used as a means of estimating the toxicity and efficacy of azathioprine treatment.

This test is performed by the Gastroenerology department. ( Phone No.9895 4632 )

The cost for the test is not covered by Medicare. 

Eastern Health patients:6TGN 6MMP Thiopurine Metabolites Testing $75

All other patients: 6TGN 6MMP Thiopurine Metabolites Testing $145

Date

Field

Changed From

Changed To

Collection & Request Instructions

Transport specimen at Room Temperature to lab

Assay name

Trichomonas

Trichomonas PCR

Alternative names

Trich, Trichomonas

Request Group

GMC

OPCR

Preferred Specimen Type

Swab

Vaginal swab
Endocervical swab
Urine

Preferred Container Type

Wound swab kit

Genital swab kit + sterile pot
Genital swab kit

Collection & Request Instructions

Urine

15 - 20 mL of first-stream urine specimen collected at least one hour since the patient last voided. Refrigerate immediately

Cervical  & Vaginal swabs

Use plastic swab in plain tube without transport medium (wire swab not suitable)

ALL Specimens must reach the Pathology laboratory within 2 hours of collection.

http://pathology.easternhealth.org.au/handbook/downloads/microbiology/Microbiology_Specimen_Collection_Guide.doc

Frequency

Daily

Twice weekly

Notes

http://pathology.easternhealth.org.au/handbook/downloads/microbiology/Microbiology_Specimen_Collection_Guide.doc

Date

Field

Changed From

Changed To

External Laboratory

Sydney South West Pathology Service(RPA)

Alfred Hospital

Frequency

As required