Services

Date

Field

Changed From

Changed To

Notes

Consultation with Endocrinologist suggested

Collect in the morning between 0800 and 1000h.

1 x 4mL EDTA tube on ice.

Send to laboratory for immediate centrifugation. Spin, separate and freeze plasma. Transport frozen.

Consultation with Endocrinologist suggested

Collect 1 x 4mL EDTA tube on ice.

Send to laboratory for immediate centrifugation. Spin, separate and freeze plasma. 

Date

Field

Changed From

Changed To

Laboratory

Referred Test

Biochemistry

Collection & Request Instructions

Collection Requirements for both or either tests

Collect into 4 mL EDTA tubes at Room Temperature. Do not collect on ice

If both Renin and Aldosterone are requested together, they can be collected into the same EDTA tube

In the laboratory, spin and separate the plasma immediately. Plasma is stable at Room Temperature if analysis is performed on the same day

If the specimen arrives on the weekend or after hours, or if the specimen is referred from an external laboratory, freeze the plasma

EDTA plasma samples from external laboratories should be stored and transported frozen

Whole blood or plasma stored at 4 Deg C is not suitable

Add-on tests for Renin and Aldosterone should not be done on samples which have been previously collected or stored at 4 Deg C

Collection Requirements for both or either tests

Collect into 4 mL EDTA tubes at Room Temperature. Do not collect on ice

If both Renin and Aldosterone are requested together, they can be collected into the same EDTA tube

In the laboratory, spin and separate the plasma immediately. Plasma is stable at Room Temperature if analysis is performed on the same day

If the specimen arrives on the weekend or after hours freeze the plasma

EDTA plasma samples from external laboratories should be stored and transported frozen

Whole blood or plasma stored at 4 Deg C is not suitable

Add-on tests for Renin and Aldosterone should not be done on samples which have been previously collected or stored at 4 Deg C

External Laboratory

Austin Pathology

Date

Field

Changed From

Changed To

External Laboratory

St Vincents Hospital

Date

Field

Changed From

Changed To

Alternative names

ADH

Request Group

ADH

Preferred Specimen Type

Plasma

Serum

Optional Specimen Type

Plasma

Preferred Container Type

EDTA

Serum tube with gel

Optional Container Type

EDTA
Lithium heparin no gel

Collection & Request Instructions

Samples should be fasting.

Processing Instructions

Spin, separate and freeze plasma.

Packed by certified personnel.

Sent frozen.

Specimen Reception, Level 6 (E Block), RPA, Missenden Road, Camperdown , NSW 2050

Tel: 02-95158279

Spin, separate and freeze serum/plasma

Packed by certified personnel.

Sent frozen.

Specimen Reception, Level 6 (E Block), RPA, Missenden Road, Camperdown , NSW 2050

Tel: 02-95158279

Date

Field

Changed From

Changed To

Processing Instructions

Place the samples and request slip in fridge (M43) in CSR. Do not enter in the computer.

Eastern Health Clinical School
Level 2, 5 Arnold Street,
Box Hill  Vic  3128
Ph: 61 3 9091 8827
Fax: 61 3 9899 9137
E-mail:  ehcs@monash.edu

Date

Field

Changed From

Changed To

Laboratory

Referred Test

Biochemistry

Preferred Container Type

Plain Tube with gel

Serum tube with gel

Collection & Request Instructions

Fasting or Non Fasting sample.

Note: This test is ONLY performed on pregnant women.

Fasting.

Note: This test is ONLY performed on pregnant women.

Processing Instructions

Spin, separate ASAP.

External Laboratory

Royal Childrens Hospital

Frequency

Weekly

Twice weekly

Method

Store and transport at 4 C.

Date

Field

Changed From

Changed To

Laboratory

Unassigned

Haematology

Date

Field

Changed From

Changed To

Notes

Transport specimen at Room Temperature to lab
Sample haemolysis may make this test unreportable

Transport specimen at Room Temperature to lab
Sample haemolysis may make this test unreportable

Send as whole blood

Date

Field

Changed From

Changed To

External Laboratory

Austin Pathology

Date

Field

Changed From

Changed To

External Laboratory

Royal Brisbane & Womens Hospital

External Transport Instructions

Transport frozen.

Packed by certified personnel.

Notes

In combination with the tumour marker CA-125, CASA is used for differentiating between benign gynaecological disease and ovarian carcinoma.  Please note that this test has changed referral laboratories as at 18/1/2011.

Please contact Chemical Pathologist before requesting this test.

In combination with the tumour marker CA-125, CASA is used for differentiating between benign gynaecological disease and ovarian carcinoma.  Please note that this test has changed referral laboratories as at 18/1/2011.  This test is no longer available in Australia

Please contact Chemical Pathologist before requesting this test.

Date

Field

Changed From

Changed To

Notes

IgG and IgM, IgG Avidity Index performed

IgG and IgM, IgG Avidity Index performed if IgM is positive

Laboratory

Microbiology

Biochemistry

Date

Field

Changed From

Changed To

Collection & Request Instructions

Requests for Alzheimer's disease - CSF must be collected in a seperate blue capped PP tube, available from Pathology Specimen Reception (CSR).

Processing Instructions

DO NOT CENTRIFUGE SPECIMEN

This test requires authorisation by the Neurology Registrar or Neurology Consultant.

CSF Specimens are unsuitable for testing if macroscopically haemorrhagic, xanthochromic, have red blood cell counts < 500/uL, have white cell counts < 10/uL

DO NOT CENTRIFUGE SPECIMEN

This test requires authorisation by the Neurology Registrar or Neurology Consultant.

CSF Specimens are unsuitable for testing if macroscopically haemorrhagic, xanthochromic. Cell count must have red blood cell counts < 500/uL, and white cell counts < 10/uL

Notes

This test requires authorisation by the Neurology Registrar or Neurology Consultant.

In addition, please refer to Eastern Health Infection Control CJD procedure and contact Infection Control prior to performing the Lumbar Puncture

Test is run weekly. Samples received by 4 p.m. Friday will have the result issued by the end of the following week.

CSF : Tests for 14-3-3 CSF protein

At least 1 mL of CSF is required in a separate (4th), unopened tube.

Double bag specimen. Must be frozen and transported on dry ice.

Blood : Test for PRNP (Prion protein gene)

5 mLs in EDTA tube

This test requires authorisation by the Neurology Registrar or Neurology Consultant.

In addition, please refer to Eastern Health Infection Control CJD procedure and contact Infection Control prior to performing the Lumbar Puncture

Test is run weekly. Samples received by 4 p.m. Friday will have the result issued by the end of the following week.

CSF : Tests for 14-3-3 CSF protein

At least 1 mL of CSF is required in a separate (4th), unopened tube.

Double bag specimen. Note: sample is no longer required to be sent frozen.

Blood : Test for PRNP (Prion protein gene)

5 mLs in EDTA tube

Date

Field

Changed From

Changed To

Collection & Request Instructions

This test required approval from the Clinical Microbiologist. Minimum 200uL/0.2mL of unspun CSF is required for this test.

This test required approval from the Clinical Microbiologist. Minimum 200uL/0.2mL of unspun CSF is required for this test.

CSF collected via lumbar punture only; bloodstained/xanthochromic samples are suitable

Notes

NOTE: Non-K1 E. coli serotypes may be present in a specimen and will not be detected by the BioFire ME Panel.
NOTE: Non-encapsulated strains of Neisseria meningitidis are not detected by the BioFire ME Panel.
NOTE: The BioFire ME Panel does not distinguish between latent and active CMV and HHV-6 infections. Detection of these viruses may indicate primary infection, secondary reactivation, or the presence of latent virus. Results should always be interpreted in conjunction with other clinical, laboratory, and epidemiological information.
NOTE: Patients with a suspicion of cryptococcal meningitis and a negative cryptococcal PCR result, such as by the BioFire ME Panel, should be tested for CrAG.

Date

Field

Changed From

Changed To

Laboratory

Microbiology

Biochemistry

Date

Field

Changed From

Changed To

Volume (Adults)

1.5 mL

1 mL

Minimum/Paediatric Volume

0.5 mL

Preferred Container Type

ESR tube

EDTA
EDTA - Paediatric

Collection & Request Instructions

Using the ESR tube :
Transport specimen at Room Temperature to lab
Sample haemolysis may make this test unreportable

Transport specimen at Room Temperature to lab
Sample haemolysis may make this test unreportable
Samples found to have clots cannot be tested

Collection & Request Instructions

This tube consists of an inner vessel with an outer sleeve.  The outer sleeve MUST remain in place as this is where sample identification (label or handwritten) will be placed. 

Frequency

Daily

Notes

If the ESR test is requested in conjunction with a FBE test request, a single EDTA tube may be shared. In these instances;

Minimum Adult Volume: 1.5 mL

Minimum Paediatric Volume: 1 mL

Date

Field

Changed From

Changed To

Volume (Adults)

20 g

Minimum/Paediatric Volume

1 g

Collection & Request Instructions

Samples can be sent frozen.

External Laboratory

Monash Pathology

Frequency

Weekly

Notes

Contact

Monash University, Eastern Health
Department of Gastroenterology
IBD Gastroenterology Laboratory
Level 1, 5 Arnold St, Box Hill  Vic  3128
Ph: 03 9895 4632
Email: ehcs.ibd@monash.edu

Link to test information and costs :

http://www.med.monash.edu.au/ehcs/services/metabolite/calprotectindrsinformation.html

Request Group

CALPRO

Date

Field

Changed From

Changed To

Alternative names

Dengue fever serology, Kunjin serology, Murray Valley Encephalitis serology

Dengue fever serology, Kunjin serology, Murray Valley Encephalitis serology, Japanese B encephalitis

Request Group

SDENG, OPCR, (SMVE, SJBE)

SDENG, SMVE, SJBE

Preferred Specimen Type

Blood

Blood
Serum

Preferred Container Type

EDTA

EDTA
Serum tube with gel

Request Group

OPCR

SDENG, OPCR, (SMVE, SJBE)

Notes

Procedure detects flaviviruses including Murray Valley encephalitis, dengue and kunjin viruses

PCR testing detects flaviviruses including Murray Valley encephalitis, dengue and kunjin viruses. Dengue specific serology tests for IgG, IgM, and NS1 antigen

Date

Field

Changed From

Changed To

Collection & Request Instructions

Processing Instructions

Spin, separate Plasma and freeze.

Packed by certified personnel.

Results:02-83829100 or 02-82829190

Spin and separate Plasma.

External Laboratory

Sydpath Pathology, St Vincent's Hospital NSW

Alfred Hospital

Date

Field

Changed From

Changed To

Processing Instructions

Perfomed by Abbott Alinity M

Method

Performed by Abbott Alinity M

Assay name

Hepatitis B Quantitation

Hepatitis B Viral Load

Alternative names

Hepatitis B Viral Load

Hepatitis B Viral Load, Hep B

Laboratory

Referred Test

Microbiology

Request Group

HEPBVL

HBVL

Processing Instructions

Contact VIDRL 9342 9615

Perfomed by Abbott Alinity M

External Laboratory

Victorian Infectious Diseases Reference Laboratory (V.I.D.R.L)

Date

Field

Changed From

Changed To

Volume (Adults)

0.3 mL

0.2 mL

Notes

Test performed is a multiplex PCR for the following viruses: Herpes simplex 1 virus (HSV-1); Herpes simplex 2 virus (HSV-2); Varicella zoster virus (VZV); Cytomegalovirus (CMV); Enterovirus (EV); human Adenovirus (hAdv). Swabs and CSF samples are performed in-house at Box Hill laboratory, other sample types are referred.

Test performed is a multiplex PCR for the following viruses: Herpes simplex 1 virus (HSV-1); Herpes simplex 2 virus (HSV-2); Varicella zoster virus (VZV); Cytomegalovirus (CMV); Enterovirus (EV); human Adenovirus (hAdv). Swabs and CSF samples are performed in-house at Box Hill laboratory, other sample types are referred.

CSF minimum volume for testing: 200uL/0.2mL

Alternative names

CMV PCR, HSV, HSV I-II, HSV PCR, VZV and CMV NAD, VZV PCR

CMV PCR, HSV, HSV I-II, HSV PCR, VZV and CMV NAD, VZV PCR, Enterovirus PCR, Adenovirus PCR

Laboratory

Referred Test

Microbiology

Request Group

HPCR

HPCR, (ENTPCR, ADEPCR)

Preferred Specimen Type

Cerebrospinal Fluid
Fluid
Tissue
Nasopharyngeal swab
Blood

Swab
Cerebrospinal Fluid
Tissue
Nasopharyngeal swab
Respiratory samples

Optional Specimen Type

Respiratory samples

Blood

External Laboratory

Monash Pathology

External Transport Instructions

Tests included in Monash PCR - Herpes simplex 1 virus, Herpes simplex 2 virus, Varicella zoster virus, Cytomegalovirus, Enterovirus (swabs only), Adenovirus (not faeces)

Specimens tested at Monash-

Swabs (dry or in UTM), Sterile fluids, Respiratory samples, Urine.

Specimens anticoagulated with heparin are unsuitable as heparin inhibits PCR

For other specimens - refer to VIDRL. Tissue, CSF, saliva, serum, Plasma (ACD or EDTA),

If  CSF Herpes and Enterovirus PCR -send to VIDRL.

Refer to Referred Molecular Test Requests WORK-MI-90

Specimens tested at Box Hill - Swabs (dry or in UTM), CSF

Specimens from other sites are referred to VIDRL for testing.

Method

Contact Monash Pathology 9594 4532 or if no answer try Bacteriology 9594 4565 as there is someone rostered 24/7

Tested M, W, F

Routine runs are performed Monday and Thursday. Additional runs for urgent CSF samples are perfomed after discussion with the Clinical Microbiologist.

Notes

This test detects Herpes Simplex Virus 1(HSV-1), Herpes Simplex Virus 2 (HSV-2), Varicella zoster virus (VZV) and Cytomegalovirus (CMV).

Test performed is a multiplex PCR for the following viruses: Herpes simplex 1 virus (HSV-1); Herpes simplex 2 virus (HSV-2); Varicella zoster virus (VZV); Cytomegalovirus (CMV); Enterovirus (EV); human Adenovirus (hAdv). Swabs and CSF samples are performed in-house at Box Hill laboratory, other sample types are referred.

Date

Field

Changed From

Changed To

External Laboratory

Austin Pathology

Date

Field

Changed From

Changed To

Preferred Specimen Type

Serum

Serum
Blood

Optional Specimen Type

Plasma

Preferred Container Type

Serum tube with gel

Serum tube with gel
EDTA
EDTA - Paediatric

Date

Field

Changed From

Changed To

Alternative names

Inflectra, Remicade

Volume (Adults)

2 mL

Processing Instructions

After centifugation the serum must be aliquoted into the special aliquot tube provided and freeze.

Store at 4 C.

External Laboratory

South Western Area Pathology,Liverpool Hospital

Dorevitch Pathology

External Transport Instructions

Samples to be batched and sent  to SSWAPs frozen

Send to Testing Laboratory at 4 C

Frequency

Monthly

Weekly

Date

Field

Changed From

Changed To

Volume (Adults)

2 mL

5 mL

Minimum/Paediatric Volume

1 mL

Preferred Container Type

Lithium heparin no gel

EDTA

Processing Instructions

Spin, separate and freeze plasma.

Spin and separate plasma.

External Laboratory

Women's & Children's Hospital (Adelaide)

Alfred Hospital

External Transport Instructions

Packed by certified personnal - transport frozen.

Results : 08-81617000, 08-81616732.

Frequency

Monthly

Twice weekly

Notes

Dose information should be provided and length of therapy must be >5 days.

Date

Field

Changed From

Changed To

Assay name

Cytogenetics / Karyotype - TISSUE

Karyotype/Microarray - TISSUE

Assay name

CytogeneticsKaryotype - TISSUE

Cytogenetics / Karyotype - TISSUE

Assay name

CYTOGENETICS - TISSUE

CytogeneticsKaryotype - TISSUE

Alternative names

KARYOTYPE CHROMOSOME STUDIES - TISSUE TISSUE CULTURE MOLECULAR KARYOTYPE MICROARRAY

KARYOTYPE CHROMOSOME STUDIES - TISSUE TISSUE CULTURE MOLECULAR KARYOTYPE MICROARRAY FIBROBLAST CULTURE

Assay name

CYTOGENETICS - TISSUE NON MALIGNANT (CONSTITUTIONAL)

CYTOGENETICS - TISSUE

Frequency

Weekly

Daily

Date

Field

Changed From

Changed To

Alternative names

Kleihauer test

Kleihauer test, FMH (feto-maternal Haemorrhage)

Request Group

KL

FMH

Date

Field

Changed From

Changed To

Alternative names

Lamictal

Volume (Adults)

2 mL

3 mL

Minimum/Paediatric Volume

1 mL

3 mL

Processing Instructions

Spin aliquot plasma. (Send either plasma or whole blood)

Spin & aliquot plasma

External Laboratory

Australian Clinical Labs (formerly Healthscope)

Alfred Hospital

Date

Field

Changed From

Changed To

Notes

Samples must be marked 'URGENT', must reach the laboratory as soon as possible. 

All out-of-hours testing requires notification.

Time and date of collection must be recorded on request form.

Whole blood - 1 x EDTA sample, do not spin. Store at room temperature. An FBE must also be requested,  and needs a separate EDTA sample.

Bone marrow aspirate - Sample into  EDTA tube

Tissue - Fresh (if to be shared with other departments for other tests eg. Histopathology or Microbiology) or RPMI fluid (especially if specimen is taken out of hours). This fluid is obtainable from: BH Lab 9895 3884, MH Lab: 9871 3572 or AH Lab: 9764 6136.

CSF/other fluids

Depending on the tests requested, 3-4 separate tubes will need to be provided. Note that the tubes are numbered so that order of collection can be identified – this order should be carefully followed, so that the optimal sample can be used for the requested tests. It is also important to consider the tests that are required, as this will determine how much CSF is needed in each tube. See the matrix below for details.

TUBE 1 is used for Biochemistry and Flow Cytometry. (Centrifuged in the lab – the supernatant is used for Biochemistry analysis (glucose, protein) and the cellular pellet will be used for flow cytometry if required). If tube 3 is bloodstained, take an aliquot for microbiology.

TUBE 2 is used for Cytology

TUBE 3 is for Microbiological analysis (cell count, Gram stain and culture) and any further infectious testing (e.g. viral PCR, mycobacterial PCR and culture, cryptococcal antigen).

TUBE 4 will be required only for;

(a) Creutzfeld-Jacob Disease (CJD) testing OR

(b) Xanthochromia analysis (wrap the tube in foil to prevent sample deterioration due to light).

Note, the ability to add further tests to a previously collected sample is dependent upon the volume provided. Also, many tests (flow cytometry, cytology) can only be performed on fresh specimens.

Other specialised testing (e.g. oligoclonal bands) will be performed using the supplied samples.

Xanthochromia and CJD analysis cannot be performed on a blood-stained sample

Samples must be marked 'URGENT', must reach the laboratory as soon as possible. 

All out-of-hours testing requires notification.

Time and date of collection must be recorded on request form.

Whole blood - 1 x EDTA sample, do not spin. Store at room temperature. An FBE and BLOOD FILM must also be requested,  and needs a separate EDTA sample.

Bone marrow aspirate - Sample into  EDTA tube

Tissue - Fresh (if to be shared with other departments for other tests eg. Histopathology or Microbiology) or RPMI fluid (especially if specimen is taken out of hours). This fluid is obtainable from: BH Lab 9895 3884, MH Lab: 9871 3572 or AH Lab: 9764 6136.

CSF/other fluids

Depending on the tests requested, 3-4 separate tubes will need to be provided. Note that the tubes are numbered so that order of collection can be identified – this order should be carefully followed, so that the optimal sample can be used for the requested tests. It is also important to consider the tests that are required, as this will determine how much CSF is needed in each tube. See the matrix below for details.

TUBE 1 is used for Biochemistry and Flow Cytometry. (Centrifuged in the lab – the supernatant is used for Biochemistry analysis (glucose, protein) and the cellular pellet will be used for flow cytometry if required). If tube 3 is bloodstained, take an aliquot for microbiology.

TUBE 2 is used for Cytology

TUBE 3 is for Microbiological analysis (cell count, Gram stain and culture) and any further infectious testing (e.g. viral PCR, mycobacterial PCR and culture, cryptococcal antigen).

TUBE 4 will be required only for;

(a) Creutzfeld-Jacob Disease (CJD) testing OR

(b) Xanthochromia analysis (wrap the tube in foil to prevent sample deterioration due to light).

Note, the ability to add further tests to a previously collected sample is dependent upon the volume provided. Also, many tests (flow cytometry, cytology) can only be performed on fresh specimens.

Other specialised testing (e.g. oligoclonal bands) will be performed using the supplied samples.

Xanthochromia and CJD analysis cannot be performed on a blood-stained sample

Notes

Samples must be marked 'URGENT', must reach the laboratory as soon as possible. 

All out-of-hours testing requires notification.

Time and date of collection must be recorded on request form.

Whole blood - 1 x EDTA sample, do not spin. Store at room temperature. An FBE must also be requested,  and needs a separate EDTA sample.

Bone marrow aspirate - Sample into  EDTA tube

Tissue - Fresh (if to be shared with other departments for other tests eg. Histopathology or Microbiology) or RPMI fluid (especially if specimen is taken out of hours). This fluid is obtainable from: BH Lab 9895 3884, MH Lab: 9871 3572 or AH Lab: 9764 6136.

CSF/other fluids - at least 2mL is preferred

Depending on the tests requested, 3-4 separate tubes will need to be provided. Note that the tubes are numbered so that order of collection can be identified – this order should be carefully followed, so that the optimal sample can be used for the requested tests. It is also important to consider the tests that are required, as this will determine how much CSF is needed in each tube. See the matrix below for details.

TUBE 1 is used for Biochemistry and Flow Cytometry. (Centrifuged in the lab – the supernatant is used for Biochemistry analysis (glucose, protein) and the cellular pellet will be used for flow cytometry if required). If tube 3 is bloodstained, take an aliquot for microbiology.

TUBE 2 is used for Cytology

TUBE 3 is for Microbiological analysis (cell count, Gram stain and culture) and any further infectious testing (e.g. viral PCR, mycobacterial PCR and culture, cryptococcal antigen).

TUBE 4 will be required only for;

(a) Creutzfeld-Jacob Disease (CJD) testing OR

(b) Xanthochromia analysis (wrap the tube in foil to prevent sample deterioration due to light).

Note, the ability to add further tests to a previously collected sample is dependent upon the volume provided. Also, many tests (flow cytometry, cytology) can only be performed on fresh specimens.

Other specialised testing (e.g. oligoclonal bands) will be performed using the supplied samples.

Xanthochromia and CJD analysis cannot be performed on a blood-stained sample

Samples must be marked 'URGENT', must reach the laboratory as soon as possible. 

All out-of-hours testing requires notification.

Time and date of collection must be recorded on request form.

Whole blood - 1 x EDTA sample, do not spin. Store at room temperature. An FBE must also be requested,  and needs a separate EDTA sample.

Bone marrow aspirate - Sample into  EDTA tube

Tissue - Fresh (if to be shared with other departments for other tests eg. Histopathology or Microbiology) or RPMI fluid (especially if specimen is taken out of hours). This fluid is obtainable from: BH Lab 9895 3884, MH Lab: 9871 3572 or AH Lab: 9764 6136.

CSF/other fluids

Depending on the tests requested, 3-4 separate tubes will need to be provided. Note that the tubes are numbered so that order of collection can be identified – this order should be carefully followed, so that the optimal sample can be used for the requested tests. It is also important to consider the tests that are required, as this will determine how much CSF is needed in each tube. See the matrix below for details.

TUBE 1 is used for Biochemistry and Flow Cytometry. (Centrifuged in the lab – the supernatant is used for Biochemistry analysis (glucose, protein) and the cellular pellet will be used for flow cytometry if required). If tube 3 is bloodstained, take an aliquot for microbiology.

TUBE 2 is used for Cytology

TUBE 3 is for Microbiological analysis (cell count, Gram stain and culture) and any further infectious testing (e.g. viral PCR, mycobacterial PCR and culture, cryptococcal antigen).

TUBE 4 will be required only for;

(a) Creutzfeld-Jacob Disease (CJD) testing OR

(b) Xanthochromia analysis (wrap the tube in foil to prevent sample deterioration due to light).

Note, the ability to add further tests to a previously collected sample is dependent upon the volume provided. Also, many tests (flow cytometry, cytology) can only be performed on fresh specimens.

Other specialised testing (e.g. oligoclonal bands) will be performed using the supplied samples.

Xanthochromia and CJD analysis cannot be performed on a blood-stained sample

Alternative names

CD4/CD8 Counts, Cell Surface Markers, FACS Analysis, Flow Cytometry

Cell Surface Markers, FACS Analysis, Flow Cytometry, Immunophenotyping

Request Group

FLOW

LYMP, LEUK

Collection & Request Instructions

Sample store at Room temperature. Samples are not stable for more than 24 hours. Hence they must be received by the Flow Cytometry lab at Box Hill within normal business hours Monday to Friday.

Cutoff for outpatient sample collection on Fridays is at 11.30 AM and MUST reach the laboratory before 4.00PM.

Flow cytometric analysis of cells stained by immunofluorescence with a range of antibodies against lymphoid and myeloid markers.

The panel of markers used will be appropriate to the clinical comments supplied , or as arranged by prior consultation.

Sample store at Room temperature. Samples are not stable for more than 24 hours. Hence they must be received by the Flow Cytometry lab at Box Hill within normal business hours Monday to Friday.

Cut-off for outpatient sample collection on Fridays is at 11.30 AM and MUST reach the laboratory before 4.00PM.

Flow cytometric analysis of cells stained by immunofluorescence with a range of antibodies against lymphoid and myeloid markers.

The panel of markers used will be appropriate to the panel selected and the clinical comments supplied , or as arranged by prior consultation

Notes

Samples must be marked 'URGENT', must reach the laboratory as soon as possible. 

All out-of-hours testing requires notification.

Time and date of collection must be recorded on request form.

Whole blood - 1 x EDTA sample, do not spin. Store at room temperature. An FBE must also be requested, needs a separate EDTA sample.

Bone marrow aspirate - Sample into  EDTA tube

Tissue - Fresh (if to be shared with other departments for other tests eg. Histopathology or Microbiology) or RPMI fluid (especially if specimen is taken out of hours). This fluid is obtainable from: BH Lab 9895 3884, MH Lab: 9871 3572 or AH Lab: 9764 6136.

CSF/other fluids - at least 2mL is preferred

Collect CSF into three separate, sterile, screw-capped tubes provided (labelled 1,2 & 3)

Tube 1 is for Biochemistry (for Protein and Glucose) 0.5ml (10 drops) required 

Tube 2 for Cytology 1.0ml (20 drops)  and/or Flow Cytometry (6ml)

For Flow Cytometry a minimum of 6ml is required  in Tube 2

For Flow Cytometry and Cytology a minimum of 7ml is required in Tube 2     

Tube 3 for Microbiology upto 10ml (Full tube)

If querying CJD the laboratory requires a 4th unopened tube containing at least 1ml of non-bloodstained CSF.

Xanthochromia investigation requires a 4th tube containing 1ml of CSF .

Samples must be marked 'URGENT', must reach the laboratory as soon as possible. 

All out-of-hours testing requires notification.

Time and date of collection must be recorded on request form.

Whole blood - 1 x EDTA sample, do not spin. Store at room temperature. An FBE must also be requested,  and needs a separate EDTA sample.

Bone marrow aspirate - Sample into  EDTA tube

Tissue - Fresh (if to be shared with other departments for other tests eg. Histopathology or Microbiology) or RPMI fluid (especially if specimen is taken out of hours). This fluid is obtainable from: BH Lab 9895 3884, MH Lab: 9871 3572 or AH Lab: 9764 6136.

CSF/other fluids - at least 2mL is preferred

Depending on the tests requested, 3-4 separate tubes will need to be provided. Note that the tubes are numbered so that order of collection can be identified – this order should be carefully followed, so that the optimal sample can be used for the requested tests. It is also important to consider the tests that are required, as this will determine how much CSF is needed in each tube. See the matrix below for details.

TUBE 1 is used for Biochemistry and Flow Cytometry. (Centrifuged in the lab – the supernatant is used for Biochemistry analysis (glucose, protein) and the cellular pellet will be used for flow cytometry if required). If tube 3 is bloodstained, take an aliquot for microbiology.

TUBE 2 is used for Cytology

TUBE 3 is for Microbiological analysis (cell count, Gram stain and culture) and any further infectious testing (e.g. viral PCR, mycobacterial PCR and culture, cryptococcal antigen).

TUBE 4 will be required only for;

(a) Creutzfeld-Jacob Disease (CJD) testing OR

(b) Xanthochromia analysis (wrap the tube in foil to prevent sample deterioration due to light).

Note, the ability to add further tests to a previously collected sample is dependent upon the volume provided. Also, many tests (flow cytometry, cytology) can only be performed on fresh specimens.

Other specialised testing (e.g. oligoclonal bands) will be performed using the supplied samples.

Xanthochromia and CJD analysis cannot be performed on a blood-stained sample

Date

Field

Changed From

Changed To

Volume (Adults)

2 mL

3 mL

Minimum/Paediatric Volume

3 mL

Optional Container Type

Lithium heparin no gel

Collection & Request Instructions

Deliver to laboratory ASAP.

A separate unopened specimen is required for this test.

Processing Instructions

Spin, separate and freeze plasma.

External Laboratory

Sydpath Pathology, St Vincent's Hospital NSW

Alfred Hospital

External Transport Instructions

Send at 4 C.

Frequency

As required

Weekly

Method

Results: Clinical Pharmacology on 02 8382 9184.

Date

Field

Changed From

Changed To

Alternative names

CD3 count, CD4 count, CD8 count, CD4:CD8 ratio CD19 count, CD56 count, flow cytometry

Alternative names

CD19 count & CD56 count, CD4 count, CD4 count, CD4:CD8 ratio Lymphocyte subsets include: CD3 count, CD4:CD8 ratio, CD8 count, CD8 count, T subsets include: CD3 count

T subsets include: CD3 count, CD4 count, CD8 count, CD4:CD8 ratio Lymphocyte subsets include: CD3 count, CD4 count, CD8 count, CD4:CD8 ratio, CD19 count & CD56 count Flow Cytometry

Collection & Request Instructions

Sample store at Room temperature. Samples are not stable for more than 24 hours. Hence they must be received by the Flow Cytometry lab at Box Hill within normal business hours Monday to Friday.

Cut-off for outpatient sample collection on Fridays is at 11.30 AM and MUST reach the laboratory before 4.00PM.

Flow cytometric analysis of cells stained by immunofluorescence with a range of antibodies against lymphoid and myeloid markers.

The panel of markers used will be appropriate to the panel selected and the clinical comments supplied, or as arranged by prior consultation.

Sample store at ROOM TEMPERATURE. Samples are not stable for more than 24 hours. Hence they must be received by the Flow Cytometry lab at Box Hill within normal business hours Monday to Friday.

Cut-off for outpatient sample collection on Fridays is at 11.30 AM and MUST reach the laboratory before 4.00PM.

Flow cytometric analysis of cells stained by immunofluorescence with a range of antibodies against lymphoid and myeloid markers.

The panel of markers used will be appropriate to the panel selected and the clinical comments supplied, or as arranged by prior consultation.

Collection & Request Instructions

The samples are not stable for more than 24 hours. Hence all samples must be recieved in the laboratory at Box Hill  Monday to Friday within business hours.

Cutoff for outpatient sample collection on Fridays is at 11.30 AM and MUST reach the laboratory before 4.00PM.

Sample store at Room temperature. Samples are not stable for more than 24 hours. Hence they must be received by the Flow Cytometry lab at Box Hill within normal business hours Monday to Friday.

Cut-off for outpatient sample collection on Fridays is at 11.30 AM and MUST reach the laboratory before 4.00PM.

Flow cytometric analysis of cells stained by immunofluorescence with a range of antibodies against lymphoid and myeloid markers.

The panel of markers used will be appropriate to the panel selected and the clinical comments supplied, or as arranged by prior consultation.

Frequency

Daily

Notes

Used to monitor T-cell numbers

Preferred EDTA sample. Do not spin, store at room temperature.

Samples must be marked 'URGENT', must reach the laboratory as soon as possible. 

All out-of-hours testing requires notification.

Time and date of collection must be recorded on request form.

Whole blood - 1 x EDTA sample, do not spin. Store at room temperature. An FBE must also be requested, and needs a separate EDTA sample.

Assay name

T Cell Subsets

Lymphocyte subsets

Alternative names

CD2, CD3, CD4, CD8

T subsets include: CD3 count, CD4 count, CD8 count, CD4:CD8 ratio Lymphocyte subsets include: CD3 count, CD4 count, CD8 count, CD4:CD8 ratio, CD19 count & CD56 count

Request Group

FLOW

TSUB & LSUB

Date

Field

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Laboratory

Microbiology

Biochemistry

Request Group

SMEA

MEA

Frequency

Daily

Twice weekly

Date

Field

Changed From

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Laboratory

Referred Test

Biochemistry

Collection & Request Instructions

Send ASAP (within 24 hrs, 7 days a week). Contact reference laboratory prior to sending the sample.

Testing performed on an as needs basis. 

External Laboratory

Peter MacCallum Cancer Institute

Method

Hand deliover samples to Biochemistry DO NOT load onto the 8100

Date

Field

Changed From

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Laboratory

Microbiology

Biochemistry

Request Group

SMUM

MUM

Frequency

Daily

Twice weekly

Date

Field

Changed From

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Collection & Request Instructions

Note: This is an URGENT send out test.

Laboratory hours are 9am - 3pm, Monday - Friday.

Note: This is an URGENT send out test.

Laboratory hours are 9am - 3pm, Monday - Friday.

Specimen must arrive at Alfred Pathology by 4pm.

External Transport Instructions

Place the specimen in an esky with ice cubes and dispatch immediately.

Place the specimen in an esky with ice cubes and dispatch immediately. Must arrive at Alfred by 4pm.

Collection & Request Instructions

Note: This is an URGENT send out test.

Laboratory hours are 9am - 5pm, Monday - Friday.

Note: This is an URGENT send out test.

Laboratory hours are 9am - 3pm, Monday - Friday.

Date

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Collection & Request Instructions

Separate specimen required.

Cutoff for outpatient sample collection on Fridays is at 11.30 AM and MUST reach the laboratory before 4.00PM.

Whole blood - 1 x EDTA sample, do not spin. Store at room temperature. An FBE and BLOOD FILM must also be requested,  and needs a separate EDTA sample.

Cutoff for outpatient sample collection on Fridays is at 11.30 AM and MUST reach the laboratory before 4.00PM.

Alternative names

CD 59, PNH flow cytometry, PNH screen

CD59, PNH screen flow cytometry, FLEAR

Alternative names

CD 59, PNH screen

CD 59, PNH screen, PNH flow cytometry

Request Group

FLOW

PNHS

Date

Field

Changed From

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Assay name

Plasma Free Heamoglobin

Plasma Free Haemoglobin

Collection & Request Instructions

Samples must be hand delivered to the lab to avoid shaking, which will falsely raise the plasma free-haemoglobin. Normal operating plasma free-haemoglobin level is < 0.10 g/dL. This is performed 24-hourly whilst on ECMO (routinely taken with the daily blood samples) according to clinical need/ECMO clinician direction.

Samples are taken by passively filling a 10mL syringe carefully from the intra-arterial line access port. Do not connect to a negative pressure “vacutainer” for sampling. The plasma free-haemoglobin collection tube is filled from the syringe after removing the lid, gently injecting blood from the syringe and then replacing the lid. No needles are required.

Preferred Specimen Type

Plasma

Serum

Preferred Container Type

Serum tube with gel

Lithium heparin no gel

Date

Field

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Method

All PCT requests must be discussed with the Chemical Pathologist upon receipt in lab, regardless of time of day or day of week. If urgent/Taxi requiring is determined, result must be phoned to the clinician upon receipt - High Risk Result.

Method

All PCT requests must be discussed with the Chemical Pathologist upon receipt in lab, regardless of time of day or day of week. If urgent/Taxi requiring is determined, result must be phoned to the clinician upon receipt - High Risk Result.

Laboratory

Biochemistry

Referred Test

Volume (Adults)

0.5 mL

External Laboratory

Royal Childrens Hospital

Date

Field

Changed From

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Collection & Request Instructions

Filled to label marker and mixed by inversion.

Patient Information - Warfarin Management

Filled to label marker and mixed by inversion.

Patient Information - Warfarin Management-English
Patient Information - Warfarin Management-Greek
Patient Information - Warfarin Management-Simplified Chinese

Date

Field

Changed From

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External Laboratory

Victorian Infectious Diseases Reference Laboratory (V.I.D.R.L)

Date

Field

Changed From

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Alternative names

QTFN, Quantiferon TB Gold

Quantiferon, TB Gold, QTFN

Frequency

Twice weekly

Weekly

Date

Field

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Collection & Request Instructions

White top tube is the preferred sample.0.2ml of blood required for each allergen.

Blood collection: still can be done after skin allergy testing.

Omega-5-Gliadin, Alpha Gal and Cefaclor : serum send to Melbourne Pathology

IgG 96 Food Allergy test is not a RAST test  It is performed at  Australian Clinical Labs.This test is NOT rebatable and costs approx  $260.

White top tube is the preferred sample.0.2ml of blood required for each allergen.

Blood collection: still can be done after skin allergy testing.

RAST for Omega-5-Gliadin to be sent to Alfred Pathology, Alpha Gal to be sent to Melbourne Pathology.                               Cefaclor and ARAh2 to be sent to Dorevitch Pathology 

IgG 96 Food Allergy test is not a RAST test  It is performed at  Australian Clinical Labs.This test is NOT rebatable and costs approx  $260.

Processing Instructions

Omega-5-Gliadin: send to Melborne Pathology

IgG 96 Food Allergy test is not a RAST test  It is performed at  Australian Clinical Labs.This test is NOT rebatable and costs approx  $260.

IgG 96 Food Allergy test is not a RAST test  It is performed at  Australian Clinical Labs.This test is NOT rebatable and costs approx  $260.

Method

RAST for Omega-5-Gliadin to be sent to Alfred Pathology, Alpha Gal to be sent to Melbourne Pathology.                               Cefaclor and ARAh2 to be sent to Dorevitch Pathology 

Date

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Collection & Request Instructions

Minimum volume of 4 ml required - this specimen cannot be used for any other purpose.

A completed "ARCBS Red Cell Reference Form" must accompany each sample sent for analysis.

This form, which can be completed online and subsequently printed to be sent with the sample can be found by clicking here 

Minimum volume of 4 ml required - this specimen cannot be used for any other purpose.

A completed "ARCBS Red Cell Reference Form" must accompany each sample sent for analysis.

This form, which can be completed online and subsequently printed to be sent with the sample can be found by clicking here 

Processing Instructions

Specimen will arrive in department at RT.  Please keep at 4 degrees prior to transport.

Specimen will arrive in department at RT.  Please keep at 4 degrees prior to transport.

External Transport Instructions

Transport at 4 degrees

Transport at 4 degrees

Date

Field

Changed From

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Laboratory

Referred Test

Biochemistry

External Laboratory

Austin Pathology

Date

Field

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Method

The following specimens will be referred for testing-

Sputum, Bronchial Washings, Bronchoalveolar Lavages - VIDRL

Swab specimens for Respiratory Multiplex PCR will be referred to Monash Molecular Laboratory

The following specimens will be referred for testing-

Sputum, Bronchial Washings, Bronchoalveolar Lavages - VIDRL

As of Feb 2023 swab specimens for Respiratory Multiplex PCR will be tested in-house.

Preferred Specimen Type

Nasopharyngeal swab
Nasopharyngeal Aspirate

Nasopharyngeal swab
NTS - nose & throat swab
Nasopharyngeal Aspirate

Optional Specimen Type

Sputum
Bronchial Washings
Broncho-alveolar Lavage

Preferred Container Type

Viral Transport Media- Nasopharyngeal
Respiratory aspiration pot

Viral Transport Media- Nasopharyngeal
Respiratory aspiration pot
respiratory PCR requirements

Collection & Request Instructions

Please note these swabs are available from stores.

Search for: VIRAL TRANSPORT MEDIA - NASOPHARYNGEAL

Pathology do not keep a stock of these swabs. Emergency departments and wards should manage a supply of these swabs according to demand.

Please note these swabs are available from stores.

Pathology do not keep a stock of these swabs. Emergency departments and wards should manage a supply of these swabs according to demand.

Method

All tests for respiratory virus PCR are considered urgent and must be delivered to the laboratory ASAP.

ICU/Oncology patients testing negative for Flu A/B and RSV  will be referred to Monash for respiratory virus panel. This includes Influenza A virus, Influenza B virus, Respiratory Syncitial Virus, Picornavirus (RV/EV), Parainfluenza viruses 1,2 and 3, Adenovirus, Human Metapneumovirus, Bordetella pertussis, Bordetella parapertussis, Mycoplasma pneumoniae, Legionella species, Chlamydiaceae.

The following specimens will be referred for testing-

Sputum, Bronchial Washings, Bronchoalveolar Lavages

Specimens for Respiratory Multiplex PCR will be referred to Monash Molecular Laboratory

Contact Monash Pathology 9594 4532 or if no answer try Bacteriology 9594 4565 as there is someone rostered 24/7

The following specimens will be referred for testing-

Sputum, Bronchial Washings, Bronchoalveolar Lavages - VIDRL

Swab specimens for Respiratory Multiplex PCR will be referred to Monash Molecular Laboratory

Notes

This PCR will detect and identify: Influenza A virus, Influenza B virus and Respiratory syncytial virus (RSV).

Transport at room temperature to the Laboratory

Testing is performed at Box Hill, Angliss, and Maroondah.

Samples received after hours may have a longer TAT due to other urgent critical pathology testing occurring.

This PCR will detect and identify: Influenza A virus, Influenza B virus and Respiratory syncytial virus (RSV).

Transport at room temperature to the Laboratory

Testing is performed at Box Hill, Angliss, and Maroondah.

DO NOT send samples in the Pathology Chute

For Rapid testing from Emergency Department - swab must be accompanied by signed Rapid Test Authorisation Form.

DO NOT call the laboratory for results/enquiries. Inpatient tests will be prioritised and results available on EMR ASAP.

Sputum samples are referred to an external laboratory - results will be delayed.

Date

Field

Changed From

Changed To

Preferred Container Type

Viral Transport Media - Nasopharyngeal

Viral Transport Media - Nasopharyngeal
respiratory PCR requirements
Viral Transport Media- Nasopharyngeal

Preferred Specimen Type

NTS - nose & throat swab
Swab

NTS - nose & throat swab
Respiratory samples

Date

Field

Changed From

Changed To

External Laboratory

Victorian Infectious Diseases Reference Laboratory (V.I.D.R.L)

Date

Field

Changed From

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Processing Instructions

Samples are be aliquoted and frozen in the barcoded tube supplied in the JCV kit

Notes

Samples are be aliquoted and frozen in the barcoded tube supplied in the JCV kit

Date

Field

Changed From

Changed To

Preferred Container Type

Lithium heparin no gel
EDTA

See Instructions

Collection & Request Instructions

Collect 1 x 4 ml Lithium Heparin tube and

1 x 4ml EDTA tube.

FBE must be requested with this test as a RCC is required for calculation of results

DO NOT SPIN.

Keep at Room Temp.

Collect 1 x 4 ml Lithium Heparin tube and

2 x 4ml EDTA tube.

FBE must be requested with this test as a RCC is required for calculation of results

DO NOT SPIN.

Keep at Room Temp.

Collection & Request Instructions

Collect 1 x 4 ml Lithium Heparin tube and 1 x 4ml EDTA tube.

FBE must be requested with this test as a RCC is required for calculation of results

DO NOT SPIN.

Keep at Room Temp.

Collect 1 x 4 ml Lithium Heparin tube and

1 x 4ml EDTA tube.

FBE must be requested with this test as a RCC is required for calculation of results

DO NOT SPIN.

Keep at Room Temp.

Request Group

TMETAB

Preferred Container Type

EDTA

Lithium heparin no gel
EDTA

Collection & Request Instructions

Transport specimen at Room Temperature to lab

Collection & Request Instructions

Collect 2x4ml EDTA blood. DO NOT SPIN

Keep at 4 deg C, do not freeze.

The metabolites are stable for upto 4 days at 4 degress.

Collect 1 x 4 ml Lithium Heparin tube and 1 x 4ml EDTA tube.

FBE must be requested with this test as a RCC is required for calculation of results

DO NOT SPIN.

Keep at Room Temp.

Processing Instructions

Keep samples at Room Temperature.

Do Not Spin. Whole Blood required

External Laboratory

St Vincents Hospital

Frequency

Weekly

Notes

Imuran is a trade name for azathioprine

The Thiopurine metabolites (6-TGN & 6-MMP) are used as a means of estimating the toxicity and efficacy of azathioprine treatment.

This test is performed by the Gastroenerology department. ( Phone No.9895 4632 )

The cost for the test is not covered by Medicare. 

Eastern Health patients:6TGN 6MMP Thiopurine Metabolites Testing $75

All other patients: 6TGN 6MMP Thiopurine Metabolites Testing $145

Imuran is a trade name for azathioprine

The Thiopurine metabolites (6-TGN & 6-MMP) are used as a means of estimating the toxicity and efficacy of azathioprine treatment.

Date

Field

Changed From

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Laboratory

Unassigned

Anatomical Pathology

External Transport Instructions

https://pathology.easternhealth.org.au/handbook/downloads/anatomical-pathology/Tissue%20Release%20Consent%20Form.doc

Date

Field

Changed From

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Request Group

STOX

TOX

Date

Field

Changed From

Changed To

Processing Instructions

The serum must be aliquoted and frozen. Transthyretin is only stable for 3 days at 4-8C .

The serum must be aliquoted and frozen. Transthyretin is only stable for 3 days at 4-8C .

External Laboratory

Monash Pathology

Date

Field

Changed From

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Alternative names

enteric fever, Typhoid fever

enteric fever, typhoid fever

Preferred Container Type

Blood Culture

Blood Culture (Adult)
Blood Culture (Paediatric)

Date

Field

Changed From

Changed To

External Laboratory

Australian Clinical Labs (formerly Healthscope)

St Vincents Hospital

Date

Field

Changed From

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External Laboratory

Australian Clinical Labs (formerly Healthscope)

St Vincents Hospital

Date

Field

Changed From

Changed To

Volume (Adults)

2 mL

1 mL

Minimum/Paediatric Volume

1 mL

Processing Instructions

Spin, separate, wrap in foil and freeze ASAP.

Transport frozen. Contact Gribbles to arrange courier.

Spin, separate, wrap in foil and freeze ASAP.

Transport frozen. 

External Laboratory

Australian Clinical Labs (formerly Healthscope)

Alfred Hospital

Frequency

Weekly

Fortnightly