Services

Date

Field

Changed From

Changed To

Notes

Consultation with Endocrinologist suggested

Collect in the morning between 0800 and 1000h.

1 x 4mL EDTA tube on ice.

Send to laboratory for immediate centrifugation. Spin, separate and freeze plasma. Transport frozen.

Consultation with Endocrinologist suggested

Collect 1 x 4mL EDTA tube on ice.

Send to laboratory for immediate centrifugation. Spin, separate and freeze plasma. 

Date

Field

Changed From

Changed To

External Laboratory

St Vincents Hospital

Date

Field

Changed From

Changed To

Alternative names

ADH

Request Group

ADH

Preferred Specimen Type

Plasma

Serum

Optional Specimen Type

Plasma

Preferred Container Type

EDTA

Serum tube with gel

Optional Container Type

EDTA
Lithium heparin no gel

Collection & Request Instructions

Samples should be fasting.

Processing Instructions

Spin, separate and freeze plasma.

Packed by certified personnel.

Sent frozen.

Specimen Reception, Level 6 (E Block), RPA, Missenden Road, Camperdown , NSW 2050

Tel: 02-95158279

Spin, separate and freeze serum/plasma

Packed by certified personnel.

Sent frozen.

Specimen Reception, Level 6 (E Block), RPA, Missenden Road, Camperdown , NSW 2050

Tel: 02-95158279

Date

Field

Changed From

Changed To

Request Group

10A: Clexane, 10AH:Plasma Heparin, APIX: Apixaban, RIVX: Rivaroxaban

10A: Clexane, 10AH:Plasma Heparin, APIX: Apixaban, RIVX: Rivaroxaban, FONX: Fondaparinux

Request Group

10A

10A: Clexane, 10AH:Plasma Heparin, APIX: Apixaban, RIVX: Rivaroxaban

Date

Field

Changed From

Changed To

Processing Instructions

Place the samples and request slip in fridge (M43) in CSR. Do not enter in the computer.

Eastern Health Clinical School
Level 2, 5 Arnold Street,
Box Hill  Vic  3128
Ph: 61 3 9091 8827
Fax: 61 3 9899 9137
E-mail:  ehcs@monash.edu

Date

Field

Changed From

Changed To

Laboratory

Referred Test

Biochemistry

Preferred Container Type

Plain Tube with gel

Serum tube with gel

Collection & Request Instructions

Fasting or Non Fasting sample.

Note: This test is ONLY performed on pregnant women.

Fasting.

Note: This test is ONLY performed on pregnant women.

Processing Instructions

Spin, separate ASAP.

External Laboratory

Royal Childrens Hospital

Frequency

Weekly

Twice weekly

Method

Store and transport at 4 C.

Date

Field

Changed From

Changed To

Processing Instructions

Bottles should be loaded on to the Bactec analyser as soon as possible. If entry is delayed, store bottles at room temperature.

Bottles should be loaded on to the Bactec FX analyser as soon as possible. If entry is delayed, store bottles at room temperature.

Processing Instructions

Bottles should be loaded on to the Virtuo analyser as soon as possible. If entry is delayed, store bottles at room temperature.

Bottles should be loaded on to the Bactec analyser as soon as possible. If entry is delayed, store bottles at room temperature.

Volume (Adults)

10 mL

8 mL

Preferred Container Type

Blood Culture

Blood Culture (Adult)

Date

Field

Changed From

Changed To

External Transport Instructions

Load as soon as possible onto the Virtuo Analyzer. Store at room temperature until loaded

Load as soon as possible onto the Bactec FX Analyzer. Store at room temperature until loaded

Volume (Adults)

4 mL

Minimum/Paediatric Volume

1 mL

Preferred Container Type

Blood Culture Paediatric

Blood Culture (Paediatric)

Collection & Request Instructions

Add 0.5 - 4mL of blood to a single aerobic paediatric bottle.

For patients with suspected bacterial endocarditis, 2 to 3 sets taken at different times should be sufficient to establish a diagnosis.

Collect blood cultures 20 minutes apart. If patient is too ill to wait 20 minutes, prior to administering antibiotics, collect the first set from one site and the second from a different site.

Please provide clinical details. Extended incubation is required for query endocarditis, brucellosis or fungal infection

Transport at room temperature

Add 1 - 3mL of blood to a single aerobic paediatric bottle.

For patients with suspected bacterial endocarditis, 2 to 3 sets taken at different times should be sufficient to establish a diagnosis.

If multiple sets required, collect blood cultures 20 minutes apart. If patient is too ill to wait 20 minutes, prior to administering antibiotics, collect the first set from one site and the second from a different site.

Please provide clinical details. Extended incubation is required for query endocarditis, brucellosis or fungal infection

Transport at room temperature

Date

Field

Changed From

Changed To

Laboratory

Unassigned

Haematology

Date

Field

Changed From

Changed To

Assay name

Bone Specific Alkiline Phosphatase

Bone Specific Alkaline Phosphatase

External Laboratory

Monash Pathology

Royal Melbourne Hospital

Date

Field

Changed From

Changed To

Request Group

CHROM

Collection & Request Instructions

Send to Peter Mac on next available courier. Stable at room temperature for a maximum of 2 weeks.

Processing Instructions

Send to Peter Mac on next available courier. Stable at room temperature for a maximum of 2 weeks.

External Transport Instructions

Room Temperature

Room Temperature

Send to Peter Mac on next available courier. Stable at room temperature for a maximum of 2 weeks.

Method

Send to Peter Mac on next available courier. Stable at room temperature for a maximum of 2 weeks.

Date

Field

Changed From

Changed To

Notes

Transport specimen at Room Temperature to lab
Sample haemolysis may make this test unreportable

Transport specimen at Room Temperature to lab
Sample haemolysis may make this test unreportable

Send as whole blood

Date

Field

Changed From

Changed To

External Laboratory

Austin Pathology

Date

Field

Changed From

Changed To

External Laboratory

Royal Brisbane & Womens Hospital

External Transport Instructions

Transport frozen.

Packed by certified personnel.

Notes

In combination with the tumour marker CA-125, CASA is used for differentiating between benign gynaecological disease and ovarian carcinoma.  Please note that this test has changed referral laboratories as at 18/1/2011.

Please contact Chemical Pathologist before requesting this test.

In combination with the tumour marker CA-125, CASA is used for differentiating between benign gynaecological disease and ovarian carcinoma.  Please note that this test has changed referral laboratories as at 18/1/2011.  This test is no longer available in Australia

Please contact Chemical Pathologist before requesting this test.

Date

Field

Changed From

Changed To

Collection & Request Instructions

Please note : to avoid sample deterioration, DO NOT send CSF specimens via the vacuum chute system.

Depending on the tests requested, 3-4 separate tubes will need to be provided.  Note that the tubes are numbered so that order of collection can be identified – this order should be carefully followed, so that the optimal sample can be used for the requested tests.  It is also important to consider the tests that are required, as this will determine how much CSF is needed in each tube.  See the matrix below for details.

TUBE 1 is used for Cytology.

TUBE 2 is used for Glucose, Protein and Flow Cytometry.

(centrifuged in the lab – the supernatant is used for Biochemistry analysis (glucose, protein) and the cellular pellet will be used for flow cytometry if required).

TUBE 3 is for Microbiological analysis (cell count, Gram stain and culture) and any further infectious testing (e.g. viral PCR, mycobacterial PCR and culture, cryptococcal antigen).

TUBE 4 will be required only for;

         (a) Creutzfeld-Jacob Disease (CJD) testing OR

(b) Xanthochromia analysis (wrap the tube in foil to prevent    sample deterioration due to light).

Note, the ability to add further tests to a previously collected sample is dependent upon the volume provided.  Also, many tests (flow cytometry, cytology) can only be performed on fresh specimens.

Other specialised testing (e.g. oligoclonal bands) will be performed using the supplied samples.

Xanthochromia and CJD analysis cannot be performed on a blood-stained sample.

CSF Information - Laboratory Processing

Depending on the tests requested, 3-4 separate tubes will need to be provided. Note that the tubes are numbered so that order of collection can be identified – this order should be carefully followed, so that the optimal sample can be used for the requested tests. It is also important to consider the tests that are required, as this will determine how much CSF is needed in each tube. See the matrix below for details.

TUBE 1 is used for Biochemistry and Flow Cytometry. (Centrifuged in the lab – the supernatant is used for Biochemistry analysis (glucose, protein) and the cellular pellet will be used for flow cytometry if required). If tube 3 is bloodstained, take an aliquot for microbiology.

TUBE 2 is used for Cytology

TUBE 3 is for Microbiological analysis (cell count, Gram stain and culture) and any further infectious testing (e.g. viral PCR, mycobacterial PCR and culture, cryptococcal antigen).

TUBE 4 will be required only for;

(a) Creutzfeld-Jacob Disease (CJD) testing OR

(b) Xanthochromia analysis (wrap the tube in foil to prevent sample deterioration due to light).

Note, the ability to add further tests to a previously collected sample is dependent upon the volume provided. Also, many tests (flow cytometry, cytology) can only be performed on fresh specimens.

Other specialised testing (e.g. oligoclonal bands) will be performed using the supplied samples.

Xanthochromia and CJD analysis cannot be performed on a blood-stained sample.

Date

Field

Changed From

Changed To

Collection & Request Instructions

The sample with the least amount of blood is prefered for Cytology.

CSF SPECIMEN INFORMATION – LABORATORY PROCESSING

Depending on the tests requested, 3-4 separate tubes will need to be provided.  Note that the tubes are numbered so that order of collection can be identified – this order should be carefully followed, so that the optimal sample can be used for the requested tests.  It is also important to consider the tests that are required, as this will determine how much CSF is needed in each tube.  See the matrix below for details.

TUBE 1 is used for Cytology.

TUBE 2 is used for Glucose, Protein and Flow Cytometry.

(centrifuged in the lab – the supernatant is used for Biochemistry analysis (glucose, protein) and the cellular pellet will be used for flow cytometry if required).

TUBE 3 is for Microbiological analysis (cell count, Gram stain and culture) and any further infectious testing (e.g. viral PCR, mycobacterial PCR and culture, cryptococcal antigen).

TUBE 4 will be required only for;

         (a) Creutzfeld-Jacob Disease (CJD) testing OR

(b) Xanthochromia analysis (wrap the tube in foil to prevent    sample deterioration due to light).

Note, the ability to add further tests to a previously collected sample is dependent upon the volume provided.  Also, many tests (flow cytometry, cytology) can only be performed on fresh specimens.

Other specialised testing (e.g. oligoclonal bands) will be performed using the supplied samples.

Xanthochromia and CJD analysis cannot be performed on a blood-stained sample.

The sample with the least amount of blood is prefered for Cytology.

CSF SPECIMEN INFORMATION – LABORATORY PROCESSING

Depending on the tests requested, 3-4 separate tubes will need to be provided.  Note that the tubes are numbered so that order of collection can be identified – this order should be carefully followed, so that the optimal sample can be used for the requested tests.  It is also important to consider the tests that are required, as this will determine how much CSF is needed in each tube.  See the matrix below for details.

TUBE 1 is used for Biochemistry and Flow Cytometry. (Centrifuged in the lab – the supernatant is used for Biochemistry analysis (glucose, protein) and the cellular pellet will be used for flow cytometry if required). If tube 3 is bloodstained, take an aliquot for microbiology.

TUBE 2 is used for Cytology

TUBE 3 is for Microbiological analysis (cell count, Gram stain and culture) and any further infectious testing (e.g. viral PCR, mycobacterial PCR and culture, cryptococcal antigen).

TUBE 4 will be required only for;

(a) Creutzfeld-Jacob Disease (CJD) testing OR

(b) Xanthochromia analysis (wrap the tube in foil to prevent sample deterioration due to light).

Note, the ability to add further tests to a previously collected sample is dependent upon the volume provided. Also, many tests (flow cytometry, cytology) can only be performed on fresh specimens.

Other specialised testing (e.g. oligoclonal bands) will be performed using the supplied samples.

Xanthochromia and CJD analysis cannot be performed on a blood-stained sample.

Method

CSF sample processing:

If Cytology/Flow Cytometry requested  : Tube 1-Biochemistry, Tube 2- Cytology/Flow Cytometry, Tube 3-Microbiology

If Cytology/Flow Cytometry not requested: Tube 1 and 3-Microbiology, Tube 2-Biochemistry

Any confusion between the terms cell count and cytology could be cleared up when the cell count is rung through to the requesting doctor by the scientist. At this point it could be determined whether cytology is a genuine request or not (all to be documented in F8 notes).

Date

Field

Changed From

Changed To

Notes

CSF SPECIMEN INFORMATION – LABORATORY PROCESSING

Depending on the tests requested, 3-4 separate tubes will need to be provided.  Note that the tubes are numbered so that order of collection can be identified – this order should be carefully followed, so that the optimal sample can be used for the requested tests.  It is also important to consider the tests that are required, as this will determine how much CSF is needed in each tube.  See the matrix below for details.

TUBE 1 is used for Cytology.

TUBE 2 is used for Glucose, Protein and Flow Cytometry.

(centrifuged in the lab – the supernatant is used for Biochemistry analysis (glucose, protein) and the cellular pellet will be used for flow cytometry if required).

TUBE 3 is for Microbiological analysis (cell count, Gram stain and culture) and any further infectious testing (e.g. viral PCR, mycobacterial PCR and culture, cryptococcal antigen).

TUBE 4 will be required only for;

         (a) Creutzfeld-Jacob Disease (CJD) testing OR

(b) Xanthochromia analysis (wrap the tube in foil to prevent    sample deterioration due to light).

Note, the ability to add further tests to a previously collected sample is dependent upon the volume provided.  Also, many tests (flow cytometry, cytology) can only be performed on fresh specimens.

Other specialised testing (e.g. oligoclonal bands) will be performed using the supplied samples.

Xanthochromia and CJD analysis cannot be performed on a blood-stained sample.

Special tests available on request:

PCR for viruses

Acid-fast stain and/or culture for Mycobacteria

Cryptococcal antigen

Serological test for Syphilis (must be paired with 5mL serum sample or test will not be performed)

Viral Serology, if CSF is accompanied by serum sample

Flow Cytometry - requires a 4th tube

The turnaround time for CSF microscopy are 30-40 minutes.

The turnaround time for CSF culture are 3-5 days.

Depending on the tests requested, 3-4 separate tubes will need to be provided. Note that the tubes are numbered so that order of collection can be identified – this order should be carefully followed, so that the optimal sample can be used for the requested tests. It is also important to consider the tests that are required, as this will determine how much CSF is needed in each tube. See the matrix below for details.

TUBE 1 is used for Biochemistry and Flow Cytometry. (Centrifuged in the lab – the supernatant is used for Biochemistry analysis (glucose, protein) and the cellular pellet will be used for flow cytometry if required). If tube 3 is bloodstained, take an aliquot for microbiology.

TUBE 2 is used for Cytology

TUBE 3 is for Microbiological analysis (cell count, Gram stain and culture) and any further infectious testing (e.g. viral PCR, mycobacterial PCR and culture, cryptococcal antigen).

TUBE 4 will be required only for;

(a) Creutzfeld-Jacob Disease (CJD) testing OR

(b) Xanthochromia analysis (wrap the tube in foil to prevent sample deterioration due to light).

Note, the ability to add further tests to a previously collected sample is dependent upon the volume provided. Also, many tests (flow cytometry, cytology) can only be performed on fresh specimens.

Other specialised testing (e.g. oligoclonal bands) will be performed using the supplied samples.

Xanthochromia and CJD analysis cannot be performed on a blood-stained sample.

Date

Field

Changed From

Changed To

Notes

Depending on the tests requested, 3-4 separate tubes will need to be provided. Note that the tubes are numbered so that order of collection can be identified – this order should be carefully followed, so that the optimal sample can be used for the requested tests. It is also important to consider the tests that are required, as this will determine how much CSF is needed in each tube. See the matrix below for details.

TUBE 1 is used for Biochemistry and Flow Cytometry. (Centrifuged in the lab – the supernatant is used for Biochemistry analysis (glucose, protein) and the cellular pellet will be used for flow cytometry if required). If tube 3 is bloodstained, take an aliquot for microbiology.

TUBE 2 is used for Cytology

TUBE 3 is for Microbiological analysis (cell count, Gram stain and culture) and any further infectious testing (e.g. viral PCR, mycobacterial PCR and culture, cryptococcal antigen).

TUBE 4 will be required only for;

(a) Creutzfeld-Jacob Disease (CJD) testing OR

(b) Xanthochromia analysis (wrap the tube in foil to prevent sample deterioration due to light).

Note, the ability to add further tests to a previously collected sample is dependent upon the volume provided. Also, many tests (flow cytometry, cytology) can only be performed on fresh specimens.

Other specialised testing (e.g. oligoclonal bands) will be performed using the supplied samples.

Xanthochromia and CJD analysis cannot be performed on a blood-stained sample.

Depending on the tests requested, 3-4 separate tubes will need to be provided. Note that the tubes are numbered so that order of collection can be identified – this order should be carefully followed, so that the optimal sample can be used for the requested tests. It is also important to consider the tests that are required, as this will determine how much CSF is needed in each tube. See the matrix below for details.

TUBE 1 is used for Biochemistry and Flow Cytometry. (Centrifuged in the lab – the supernatant is used for Biochemistry analysis (glucose, protein) and the cellular pellet will be used for flow cytometry if required). If tube 3 is bloodstained, take an aliquot for microbiology.

TUBE 2 is used for Cytology

TUBE 3 is for Microbiological analysis (cell count, Gram stain and culture) and any further infectious testing (e.g. viral PCR, mycobacterial PCR and culture, cryptococcal antigen).

TUBE 4 will be required only for;

(a) Creutzfeld-Jacob Disease (CJD) testing OR

(b) Xanthochromia analysis (wrap the tube in foil to prevent sample deterioration due to light).

Note, the ability to add further tests to a previously collected sample is dependent upon the volume provided. Also, many tests (flow cytometry, cytology) can only be performed on fresh specimens.

Other specialised testing (e.g. oligoclonal bands) will be performed using the supplied samples.

Xanthochromia and CJD analysis cannot be performed on a blood-stained sample.

Notes

CSF SPECIMEN INFORMATION – LABORATORY PROCESSING

Depending on the tests requested, 3-4 separate tubes will need to be provided.  Note that the tubes are numbered so that order of collection can be identified – this order should be carefully followed, so that the optimal sample can be used for the requested tests.  It is also important to consider the tests that are required, as this will determine how much CSF is needed in each tube.  See the matrix below for details.

TUBE 1 is used for Cytology.

TUBE 2 is used for Glucose, Protein and Flow Cytometry.

(centrifuged in the lab – the supernatant is used for Biochemistry analysis (glucose, protein) and the cellular pellet will be used for flow cytometry if required).

TUBE 3 is for Microbiological analysis (cell count, Gram stain and culture) and any further infectious testing (e.g. viral PCR, mycobacterial PCR and culture, cryptococcal antigen).

TUBE 4 will be required only for;

         (a) Creutzfeld-Jacob Disease (CJD) testing OR

(b) Xanthochromia analysis (wrap the tube in foil to prevent    sample deterioration due to light).

Note, the ability to add further tests to a previously collected sample is dependent upon the volume provided.  Also, many tests (flow cytometry, cytology) can only be performed on fresh specimens.

Other specialised testing (e.g. oligoclonal bands) will be performed using the supplied samples.

Xanthochromia and CJD analysis cannot be performed on a blood-stained sample.

Depending on the tests requested, 3-4 separate tubes will need to be provided. Note that the tubes are numbered so that order of collection can be identified – this order should be carefully followed, so that the optimal sample can be used for the requested tests. It is also important to consider the tests that are required, as this will determine how much CSF is needed in each tube. See the matrix below for details.

TUBE 1 is used for Biochemistry and Flow Cytometry. (Centrifuged in the lab – the supernatant is used for Biochemistry analysis (glucose, protein) and the cellular pellet will be used for flow cytometry if required). If tube 3 is bloodstained, take an aliquot for microbiology.

TUBE 2 is used for Cytology

TUBE 3 is for Microbiological analysis (cell count, Gram stain and culture) and any further infectious testing (e.g. viral PCR, mycobacterial PCR and culture, cryptococcal antigen).

TUBE 4 will be required only for;

(a) Creutzfeld-Jacob Disease (CJD) testing OR

(b) Xanthochromia analysis (wrap the tube in foil to prevent sample deterioration due to light).

Note, the ability to add further tests to a previously collected sample is dependent upon the volume provided. Also, many tests (flow cytometry, cytology) can only be performed on fresh specimens.

Other specialised testing (e.g. oligoclonal bands) will be performed using the supplied samples.

Xanthochromia and CJD analysis cannot be performed on a blood-stained sample.

Date

Field

Changed From

Changed To

Notes

IgG and IgM, IgG Avidity Index performed

IgG and IgM, IgG Avidity Index performed if IgM is positive

Laboratory

Microbiology

Biochemistry

Date

Field

Changed From

Changed To

Laboratory

Referred Test

Coagulation

Request Group

CBA

Collection & Request Instructions

Fill to label marker and mix by inversion.

Do not use 1 mL citrate tubes.

Fill to label marker and mix by inversion.

Do not use 1 mL citrate tubes.

Processing Instructions

Specimens kept at room temperature, should be centrifuged ASAP and tested within 4 hours from time of specimen collection. If testing is not completed within 4 hours, plasma should be removed from cells and aliquoted (1mL) into Eppendorf tubes, 2 X 1 mL vials per CBA assay.

Plasma may be frozen @ -20 C for up to 2 weeks or -70 C for up to 6 months. Frost free freezers should not be used.

Specimens kept at room temperature, should be centrifuged ASAP and tested within 4 hours from time of specimen collection. If testing is not completed within 4 hours, plasma should be removed from cells and aliquoted (1mL) into Eppendorf tubes, 2 X 1 mL vials per CBA assay.

Plasma may be frozen @ -20 C for up to 2 weeks or -70 C for up to 6 months. Frost free freezers should not be used.

External Laboratory

Austin Pathology

Frequency

Fortnightly

Date

Field

Changed From

Changed To

External Laboratory

Australian Clinical Labs (formerly Healthscope)

Pacific Laboratory Medicine Services

Date

Field

Changed From

Changed To

Laboratory

Microbiology

Biochemistry

Date

Field

Changed From

Changed To

Volume (Adults)

20 g

Minimum/Paediatric Volume

1 g

Collection & Request Instructions

Samples can be sent frozen.

External Laboratory

Monash Pathology

Frequency

Weekly

Notes

Contact

Monash University, Eastern Health
Department of Gastroenterology
IBD Gastroenterology Laboratory
Level 1, 5 Arnold St, Box Hill  Vic  3128
Ph: 03 9895 4632
Email: ehcs.ibd@monash.edu

Link to test information and costs :

http://www.med.monash.edu.au/ehcs/services/metabolite/calprotectindrsinformation.html

Request Group

CALPRO

Date

Field

Changed From

Changed To

Alternative names

Dengue fever serology, Kunjin serology, Murray Valley Encephalitis serology

Dengue fever serology, Kunjin serology, Murray Valley Encephalitis serology, Japanese B encephalitis

Request Group

SDENG, OPCR, (SMVE, SJBE)

SDENG, SMVE, SJBE

Preferred Specimen Type

Blood

Blood
Serum

Preferred Container Type

EDTA

EDTA
Serum tube with gel

Request Group

OPCR

SDENG, OPCR, (SMVE, SJBE)

Notes

Procedure detects flaviviruses including Murray Valley encephalitis, dengue and kunjin viruses

PCR testing detects flaviviruses including Murray Valley encephalitis, dengue and kunjin viruses. Dengue specific serology tests for IgG, IgM, and NS1 antigen

Date

Field

Changed From

Changed To

Collection & Request Instructions

Processing Instructions

Spin, separate Plasma and freeze.

Packed by certified personnel.

Results:02-83829100 or 02-82829190

Spin and separate Plasma.

External Laboratory

Sydpath Pathology, St Vincent's Hospital NSW

Alfred Hospital

Date

Field

Changed From

Changed To

External Laboratory

Melbourne Pathology

Method

Free PSA is now performed in house at Box Hill Hospital.

Chemical Pathologist approval is still required

Date

Field

Changed From

Changed To

Processing Instructions

Perfomed by Abbott Alinity M

Method

Performed by Abbott Alinity M

Assay name

Hepatitis B Quantitation

Hepatitis B Viral Load

Alternative names

Hepatitis B Viral Load

Hepatitis B Viral Load, Hep B

Laboratory

Referred Test

Microbiology

Request Group

HEPBVL

HBVL

Processing Instructions

Contact VIDRL 9342 9615

Perfomed by Abbott Alinity M

External Laboratory

Victorian Infectious Diseases Reference Laboratory (V.I.D.R.L)

Date

Field

Changed From

Changed To

External Laboratory

Austin Pathology

Date

Field

Changed From

Changed To

Preferred Specimen Type

Serum

Serum
Blood

Optional Specimen Type

Plasma

Preferred Container Type

Serum tube with gel

Serum tube with gel
EDTA
EDTA - Paediatric

Date

Field

Changed From

Changed To

Alternative names

Inflectra, Remicade

Volume (Adults)

2 mL

Processing Instructions

After centifugation the serum must be aliquoted into the special aliquot tube provided and freeze.

Store at 4 C.

External Laboratory

South Western Area Pathology,Liverpool Hospital

Dorevitch Pathology

External Transport Instructions

Samples to be batched and sent  to SSWAPs frozen

Send to Testing Laboratory at 4 C

Frequency

Monthly

Weekly

Date

Field

Changed From

Changed To

Collection & Request Instructions

Liver biopsy specimen to be placed directly into a clear, dry container and delivered to the laboraory as soon as possible. Minimum sample size for accurate analysis is 1-2 mg.

Liver biopsy specimen to be placed directly into a clear, dry container and delivered to the laboraory as soon as possible. Minimum sample size for accurate analysis is 1-2 mg.

External Laboratory

Australian Clinical Labs (formerly Healthscope)

Pacific Laboratory Medicine Services

Notes

Values > 2 indicate haemochromatosis

Values > 2 indicate haemochromatosis

Liver biopsy specimen to be placed directly into a clear, dry container and delivered to the laboraory as soon as possible. Minimum sample size for accurate analysis is 1-2 mg.

Date

Field

Changed From

Changed To

Volume (Adults)

2 mL

5 mL

Minimum/Paediatric Volume

1 mL

Preferred Container Type

Lithium heparin no gel

EDTA

Processing Instructions

Spin, separate and freeze plasma.

Spin and separate plasma.

External Laboratory

Women's & Children's Hospital (Adelaide)

Alfred Hospital

External Transport Instructions

Packed by certified personnal - transport frozen.

Results : 08-81617000, 08-81616732.

Frequency

Monthly

Twice weekly

Notes

Dose information should be provided and length of therapy must be >5 days.

Date

Field

Changed From

Changed To

Assay name

Cytogenetics / Karyotype - TISSUE

Karyotype/Microarray - TISSUE

Assay name

CytogeneticsKaryotype - TISSUE

Cytogenetics / Karyotype - TISSUE

Assay name

CYTOGENETICS - TISSUE

CytogeneticsKaryotype - TISSUE

Alternative names

KARYOTYPE CHROMOSOME STUDIES - TISSUE TISSUE CULTURE MOLECULAR KARYOTYPE MICROARRAY

KARYOTYPE CHROMOSOME STUDIES - TISSUE TISSUE CULTURE MOLECULAR KARYOTYPE MICROARRAY FIBROBLAST CULTURE

Assay name

CYTOGENETICS - TISSUE NON MALIGNANT (CONSTITUTIONAL)

CYTOGENETICS - TISSUE

Frequency

Weekly

Daily

Date

Field

Changed From

Changed To

Alternative names

Lamictal

Volume (Adults)

2 mL

3 mL

Minimum/Paediatric Volume

1 mL

3 mL

Processing Instructions

Spin aliquot plasma. (Send either plasma or whole blood)

Spin & aliquot plasma

External Laboratory

Australian Clinical Labs (formerly Healthscope)

Alfred Hospital

Date

Field

Changed From

Changed To

Notes

Samples must be marked 'URGENT', must reach the laboratory as soon as possible. 

All out-of-hours testing requires notification.

Time and date of collection must be recorded on request form.

Whole blood - 1 x EDTA sample, do not spin. Store at room temperature. An FBE must also be requested,  and needs a separate EDTA sample.

Bone marrow aspirate - Sample into  EDTA tube

Tissue - Fresh (if to be shared with other departments for other tests eg. Histopathology or Microbiology) or RPMI fluid (especially if specimen is taken out of hours). This fluid is obtainable from: BH Lab 9895 3884, MH Lab: 9871 3572 or AH Lab: 9764 6136.

CSF/other fluids

Depending on the tests requested, 3-4 separate tubes will need to be provided. Note that the tubes are numbered so that order of collection can be identified – this order should be carefully followed, so that the optimal sample can be used for the requested tests. It is also important to consider the tests that are required, as this will determine how much CSF is needed in each tube. See the matrix below for details.

TUBE 1 is used for Biochemistry and Flow Cytometry. (Centrifuged in the lab – the supernatant is used for Biochemistry analysis (glucose, protein) and the cellular pellet will be used for flow cytometry if required). If tube 3 is bloodstained, take an aliquot for microbiology.

TUBE 2 is used for Cytology

TUBE 3 is for Microbiological analysis (cell count, Gram stain and culture) and any further infectious testing (e.g. viral PCR, mycobacterial PCR and culture, cryptococcal antigen).

TUBE 4 will be required only for;

(a) Creutzfeld-Jacob Disease (CJD) testing OR

(b) Xanthochromia analysis (wrap the tube in foil to prevent sample deterioration due to light).

Note, the ability to add further tests to a previously collected sample is dependent upon the volume provided. Also, many tests (flow cytometry, cytology) can only be performed on fresh specimens.

Other specialised testing (e.g. oligoclonal bands) will be performed using the supplied samples.

Xanthochromia and CJD analysis cannot be performed on a blood-stained sample

Samples must be marked 'URGENT', must reach the laboratory as soon as possible. 

All out-of-hours testing requires notification.

Time and date of collection must be recorded on request form.

Whole blood - 1 x EDTA sample, do not spin. Store at room temperature. An FBE and BLOOD FILM must also be requested,  and needs a separate EDTA sample.

Bone marrow aspirate - Sample into  EDTA tube

Tissue - Fresh (if to be shared with other departments for other tests eg. Histopathology or Microbiology) or RPMI fluid (especially if specimen is taken out of hours). This fluid is obtainable from: BH Lab 9895 3884, MH Lab: 9871 3572 or AH Lab: 9764 6136.

CSF/other fluids

Depending on the tests requested, 3-4 separate tubes will need to be provided. Note that the tubes are numbered so that order of collection can be identified – this order should be carefully followed, so that the optimal sample can be used for the requested tests. It is also important to consider the tests that are required, as this will determine how much CSF is needed in each tube. See the matrix below for details.

TUBE 1 is used for Biochemistry and Flow Cytometry. (Centrifuged in the lab – the supernatant is used for Biochemistry analysis (glucose, protein) and the cellular pellet will be used for flow cytometry if required). If tube 3 is bloodstained, take an aliquot for microbiology.

TUBE 2 is used for Cytology

TUBE 3 is for Microbiological analysis (cell count, Gram stain and culture) and any further infectious testing (e.g. viral PCR, mycobacterial PCR and culture, cryptococcal antigen).

TUBE 4 will be required only for;

(a) Creutzfeld-Jacob Disease (CJD) testing OR

(b) Xanthochromia analysis (wrap the tube in foil to prevent sample deterioration due to light).

Note, the ability to add further tests to a previously collected sample is dependent upon the volume provided. Also, many tests (flow cytometry, cytology) can only be performed on fresh specimens.

Other specialised testing (e.g. oligoclonal bands) will be performed using the supplied samples.

Xanthochromia and CJD analysis cannot be performed on a blood-stained sample

Notes

Samples must be marked 'URGENT', must reach the laboratory as soon as possible. 

All out-of-hours testing requires notification.

Time and date of collection must be recorded on request form.

Whole blood - 1 x EDTA sample, do not spin. Store at room temperature. An FBE must also be requested,  and needs a separate EDTA sample.

Bone marrow aspirate - Sample into  EDTA tube

Tissue - Fresh (if to be shared with other departments for other tests eg. Histopathology or Microbiology) or RPMI fluid (especially if specimen is taken out of hours). This fluid is obtainable from: BH Lab 9895 3884, MH Lab: 9871 3572 or AH Lab: 9764 6136.

CSF/other fluids - at least 2mL is preferred

Depending on the tests requested, 3-4 separate tubes will need to be provided. Note that the tubes are numbered so that order of collection can be identified – this order should be carefully followed, so that the optimal sample can be used for the requested tests. It is also important to consider the tests that are required, as this will determine how much CSF is needed in each tube. See the matrix below for details.

TUBE 1 is used for Biochemistry and Flow Cytometry. (Centrifuged in the lab – the supernatant is used for Biochemistry analysis (glucose, protein) and the cellular pellet will be used for flow cytometry if required). If tube 3 is bloodstained, take an aliquot for microbiology.

TUBE 2 is used for Cytology

TUBE 3 is for Microbiological analysis (cell count, Gram stain and culture) and any further infectious testing (e.g. viral PCR, mycobacterial PCR and culture, cryptococcal antigen).

TUBE 4 will be required only for;

(a) Creutzfeld-Jacob Disease (CJD) testing OR

(b) Xanthochromia analysis (wrap the tube in foil to prevent sample deterioration due to light).

Note, the ability to add further tests to a previously collected sample is dependent upon the volume provided. Also, many tests (flow cytometry, cytology) can only be performed on fresh specimens.

Other specialised testing (e.g. oligoclonal bands) will be performed using the supplied samples.

Xanthochromia and CJD analysis cannot be performed on a blood-stained sample

Samples must be marked 'URGENT', must reach the laboratory as soon as possible. 

All out-of-hours testing requires notification.

Time and date of collection must be recorded on request form.

Whole blood - 1 x EDTA sample, do not spin. Store at room temperature. An FBE must also be requested,  and needs a separate EDTA sample.

Bone marrow aspirate - Sample into  EDTA tube

Tissue - Fresh (if to be shared with other departments for other tests eg. Histopathology or Microbiology) or RPMI fluid (especially if specimen is taken out of hours). This fluid is obtainable from: BH Lab 9895 3884, MH Lab: 9871 3572 or AH Lab: 9764 6136.

CSF/other fluids

Depending on the tests requested, 3-4 separate tubes will need to be provided. Note that the tubes are numbered so that order of collection can be identified – this order should be carefully followed, so that the optimal sample can be used for the requested tests. It is also important to consider the tests that are required, as this will determine how much CSF is needed in each tube. See the matrix below for details.

TUBE 1 is used for Biochemistry and Flow Cytometry. (Centrifuged in the lab – the supernatant is used for Biochemistry analysis (glucose, protein) and the cellular pellet will be used for flow cytometry if required). If tube 3 is bloodstained, take an aliquot for microbiology.

TUBE 2 is used for Cytology

TUBE 3 is for Microbiological analysis (cell count, Gram stain and culture) and any further infectious testing (e.g. viral PCR, mycobacterial PCR and culture, cryptococcal antigen).

TUBE 4 will be required only for;

(a) Creutzfeld-Jacob Disease (CJD) testing OR

(b) Xanthochromia analysis (wrap the tube in foil to prevent sample deterioration due to light).

Note, the ability to add further tests to a previously collected sample is dependent upon the volume provided. Also, many tests (flow cytometry, cytology) can only be performed on fresh specimens.

Other specialised testing (e.g. oligoclonal bands) will be performed using the supplied samples.

Xanthochromia and CJD analysis cannot be performed on a blood-stained sample

Alternative names

CD4/CD8 Counts, Cell Surface Markers, FACS Analysis, Flow Cytometry

Cell Surface Markers, FACS Analysis, Flow Cytometry, Immunophenotyping

Request Group

FLOW

LYMP, LEUK

Collection & Request Instructions

Sample store at Room temperature. Samples are not stable for more than 24 hours. Hence they must be received by the Flow Cytometry lab at Box Hill within normal business hours Monday to Friday.

Cutoff for outpatient sample collection on Fridays is at 11.30 AM and MUST reach the laboratory before 4.00PM.

Flow cytometric analysis of cells stained by immunofluorescence with a range of antibodies against lymphoid and myeloid markers.

The panel of markers used will be appropriate to the clinical comments supplied , or as arranged by prior consultation.

Sample store at Room temperature. Samples are not stable for more than 24 hours. Hence they must be received by the Flow Cytometry lab at Box Hill within normal business hours Monday to Friday.

Cut-off for outpatient sample collection on Fridays is at 11.30 AM and MUST reach the laboratory before 4.00PM.

Flow cytometric analysis of cells stained by immunofluorescence with a range of antibodies against lymphoid and myeloid markers.

The panel of markers used will be appropriate to the panel selected and the clinical comments supplied , or as arranged by prior consultation

Notes

Samples must be marked 'URGENT', must reach the laboratory as soon as possible. 

All out-of-hours testing requires notification.

Time and date of collection must be recorded on request form.

Whole blood - 1 x EDTA sample, do not spin. Store at room temperature. An FBE must also be requested, needs a separate EDTA sample.

Bone marrow aspirate - Sample into  EDTA tube

Tissue - Fresh (if to be shared with other departments for other tests eg. Histopathology or Microbiology) or RPMI fluid (especially if specimen is taken out of hours). This fluid is obtainable from: BH Lab 9895 3884, MH Lab: 9871 3572 or AH Lab: 9764 6136.

CSF/other fluids - at least 2mL is preferred

Collect CSF into three separate, sterile, screw-capped tubes provided (labelled 1,2 & 3)

Tube 1 is for Biochemistry (for Protein and Glucose) 0.5ml (10 drops) required 

Tube 2 for Cytology 1.0ml (20 drops)  and/or Flow Cytometry (6ml)

For Flow Cytometry a minimum of 6ml is required  in Tube 2

For Flow Cytometry and Cytology a minimum of 7ml is required in Tube 2     

Tube 3 for Microbiology upto 10ml (Full tube)

If querying CJD the laboratory requires a 4th unopened tube containing at least 1ml of non-bloodstained CSF.

Xanthochromia investigation requires a 4th tube containing 1ml of CSF .

Samples must be marked 'URGENT', must reach the laboratory as soon as possible. 

All out-of-hours testing requires notification.

Time and date of collection must be recorded on request form.

Whole blood - 1 x EDTA sample, do not spin. Store at room temperature. An FBE must also be requested,  and needs a separate EDTA sample.

Bone marrow aspirate - Sample into  EDTA tube

Tissue - Fresh (if to be shared with other departments for other tests eg. Histopathology or Microbiology) or RPMI fluid (especially if specimen is taken out of hours). This fluid is obtainable from: BH Lab 9895 3884, MH Lab: 9871 3572 or AH Lab: 9764 6136.

CSF/other fluids - at least 2mL is preferred

Depending on the tests requested, 3-4 separate tubes will need to be provided. Note that the tubes are numbered so that order of collection can be identified – this order should be carefully followed, so that the optimal sample can be used for the requested tests. It is also important to consider the tests that are required, as this will determine how much CSF is needed in each tube. See the matrix below for details.

TUBE 1 is used for Biochemistry and Flow Cytometry. (Centrifuged in the lab – the supernatant is used for Biochemistry analysis (glucose, protein) and the cellular pellet will be used for flow cytometry if required). If tube 3 is bloodstained, take an aliquot for microbiology.

TUBE 2 is used for Cytology

TUBE 3 is for Microbiological analysis (cell count, Gram stain and culture) and any further infectious testing (e.g. viral PCR, mycobacterial PCR and culture, cryptococcal antigen).

TUBE 4 will be required only for;

(a) Creutzfeld-Jacob Disease (CJD) testing OR

(b) Xanthochromia analysis (wrap the tube in foil to prevent sample deterioration due to light).

Note, the ability to add further tests to a previously collected sample is dependent upon the volume provided. Also, many tests (flow cytometry, cytology) can only be performed on fresh specimens.

Other specialised testing (e.g. oligoclonal bands) will be performed using the supplied samples.

Xanthochromia and CJD analysis cannot be performed on a blood-stained sample

Assay name

Leucocyte Immunophenotyping

T Cell Subsets

Alternative names

CD4/CD8 Counts, Cell Surface Markers, FACS Analysis, Flow Cytometry

CD2, CD3, CD4, CD8

Preferred Container Type

EDTA
Sterile Jar

EDTA
Lithium heparin no gel

Collection & Request Instructions

Sample store at Room temperature. Samples are not stable for more than 24 hours. Hence they must be received by the Flow Cytometry lab at Box Hill within normal business hours Monday to Friday.

Cutoff for outpatient sample collection on Fridays is at 11.30 AM and MUST reach the laboratory before 4.00PM.

Flow cytometric analysis of cells stained by immunofluorescence with a range of antibodies against lymphoid and myeloid markers.

The panel of markers used will be appropriate to the clinical comments supplied , or as arranged by prior consultation.

The samples are not stable for more than 24 hours. Hence all samples must be recieved in the laboratory at Box Hill  Monday to Friday within business hours.

Cutoff for outpatient sample collection on Fridays is at 11.30 AM and MUST reach the laboratory before 4.00PM.

Processing Instructions

Prefer EDTA sample.

Do not spin, store at room temperature.

Notes

Samples must be marked 'URGENT', must reach the laboratory as soon as possible. 

All out-of-hours testing requires notification.

Time and date of collection must be recorded on request form.

Whole blood - 1 x EDTA sample, do not spin. Store at room temperature. An FBE must also be requested, needs a separate EDTA sample.

Bone marrow aspirate - Sample into  EDTA tube

Tissue - Fresh (if to be shared with other departments for other tests eg. Histopathology or Microbiology) or RPMI fluid (especially if specimen is taken out of hours). This fluid is obtainable from: BH Lab 9895 3884, MH Lab: 9871 3572 or AH Lab: 9764 6136.

CSF/other fluids - at least 2mL is preferred

Collect CSF into three separate, sterile, screw-capped tubes provided (labelled 1,2 & 3)

Tube 1 is for Biochemistry (for Protein and Glucose) 0.5ml (10 drops) required 

Tube 2 for Cytology 1.0ml (20 drops)  and/or Flow Cytometry (6ml)

For Flow Cytometry a minimum of 6ml is required  in Tube 2

For Flow Cytometry and Cytology a minimum of 7ml is required in Tube 2     

Tube 3 for Microbiology upto 10ml (Full tube)

If querying CJD the laboratory requires a 4th unopened tube containing at least 1ml of non-bloodstained CSF.

Xanthochromia investigation requires a 4th tube containing 1ml of CSF .

Used to monitor T-cell numbers

Preferred EDTA sample. Do not spin, store at room temperature.

Collection & Request Instructions

Sample store at Room temperature. Samples are not stable for more than 24 hours. Hence they must be recieved by the Flow Cytometry lab at Box Hill within normal business hours Monday to Friday.

Cutoff for outpatirnt sample collection on Fridays is at 12:00 PM and MUST reach the laboratory by 4.00PM.

Flow cytometric analysis of cells stained by immunofluorescence with a range of antibodies against lymphoid and myeloid markers.

The panel of markers used will be appropriate to the clinical comments supplied , or as arranged by prior consultation.

Sample store at Room temperature. Samples are not stable for more than 24 hours. Hence they must be received by the Flow Cytometry lab at Box Hill within normal business hours Monday to Friday.

Cutoff for outpatient sample collection on Fridays is at 11.30 AM and MUST reach the laboratory before 4.00PM.

Flow cytometric analysis of cells stained by immunofluorescence with a range of antibodies against lymphoid and myeloid markers.

The panel of markers used will be appropriate to the clinical comments supplied , or as arranged by prior consultation.

Collection & Request Instructions

Sample store at Room temperature. Samples are not stable for more than 24 hours. Hence they must be recieved by the Flow Cytometry lab at Box Hill within normal business hours Monday to Friday

Flow cytometric analysis of cells stained by immunofluorescence with a range of antibodies against lymphoid and myeloid markers.

The panel of markers used will be appropriate to the clinical comments supplied , or as arranged by prior consultation.

Sample store at Room temperature. Samples are not stable for more than 24 hours. Hence they must be recieved by the Flow Cytometry lab at Box Hill within normal business hours Monday to Friday.

Cutoff for outpatirnt sample collection on Fridays is at 12:00 PM and MUST reach the laboratory by 4.00PM.

Flow cytometric analysis of cells stained by immunofluorescence with a range of antibodies against lymphoid and myeloid markers.

The panel of markers used will be appropriate to the clinical comments supplied , or as arranged by prior consultation.

Notes

• Samples must be marked 'URGENT', must reach the laboratory as soon as possible. 
• All out-of-hours testing requires notification.
• Time and date of collection must be recorded on request form.

  Whole blood - 1 x EDTA sample, do not spin. Store at room temperature. An FBE must also be requested, needs a separate EDTA sample.

  Bone marrow aspirate - Sample into  EDTA tube

  Tissue - Fresh (if to be shared with other departments for other tests eg. Histopathology or Microbiology) or RPMI fluid (especially if specimen is taken out of hours). This fluid is obtainable from: BH Lab 9895 3884, MH Lab: 9871 3572 or AH Lab: 9764 6136.

• CSF/other fluids - at least 2mL is preferred

• Collect CSF into three separate, sterile, screw-capped tubes provided (labelled 1,2 & 3)

• Tube 1 is for Biochemistry (for Protein and Glucose) 0.5ml (10 drops) required 
• Tube 2 for Cytology 1.0ml (20 drops)  and/or Flow Cytometry (6ml)
  • For Flow Cytometry a minimum of 6ml is required  in Tube 2
  • For Flow Cytometry and Cytology a minimum of 7ml is required in Tube 2     
• Tube 3 for Microbiology upto 10ml (Full tube)

  If querying CJD the laboratory requires a 4th unopened tube containing at least 1ml of non-bloodstained CSF.

  Xanthochromia investigation requires a 4th tube containing 1ml of CSF .

Samples must be marked 'URGENT', must reach the laboratory as soon as possible. 

All out-of-hours testing requires notification.

Time and date of collection must be recorded on request form.

Whole blood - 1 x EDTA sample, do not spin. Store at room temperature. An FBE must also be requested, needs a separate EDTA sample.

Bone marrow aspirate - Sample into  EDTA tube

Tissue - Fresh (if to be shared with other departments for other tests eg. Histopathology or Microbiology) or RPMI fluid (especially if specimen is taken out of hours). This fluid is obtainable from: BH Lab 9895 3884, MH Lab: 9871 3572 or AH Lab: 9764 6136.

CSF/other fluids - at least 2mL is preferred

Collect CSF into three separate, sterile, screw-capped tubes provided (labelled 1,2 & 3)

Tube 1 is for Biochemistry (for Protein and Glucose) 0.5ml (10 drops) required 

Tube 2 for Cytology 1.0ml (20 drops)  and/or Flow Cytometry (6ml)

For Flow Cytometry a minimum of 6ml is required  in Tube 2

For Flow Cytometry and Cytology a minimum of 7ml is required in Tube 2     

Tube 3 for Microbiology upto 10ml (Full tube)

If querying CJD the laboratory requires a 4th unopened tube containing at least 1ml of non-bloodstained CSF.

Xanthochromia investigation requires a 4th tube containing 1ml of CSF .

Date

Field

Changed From

Changed To

Volume (Adults)

2 mL

3 mL

Minimum/Paediatric Volume

3 mL

Optional Container Type

Lithium heparin no gel

Collection & Request Instructions

Deliver to laboratory ASAP.

A separate unopened specimen is required for this test.

Processing Instructions

Spin, separate and freeze plasma.

External Laboratory

Sydpath Pathology, St Vincent's Hospital NSW

Alfred Hospital

External Transport Instructions

Send at 4 C.

Frequency

As required

Weekly

Method

Results: Clinical Pharmacology on 02 8382 9184.

Date

Field

Changed From

Changed To

External Transport Instructions

Samples can be transported frozen to the laboratory if the facilities are available to double spin and aliquot these sample. This has to be done within 4 hours.

Unspun sample is stable for up to 4 hours and must be transport to laboratory immediately. (Sample must be double spun, aliquoted and frozen within 4 hours from time of collection).

Samples can be transported frozen to the laboratory if the facilities are available to double spin and aliquot these samples. This has to be performed within 4 hours of collection.

Unspun sample is stable for up to 4 hours and must be transport to laboratory immediately. (Sample must be double spun, aliquoted and frozen within 4 hours from time of collection).

Collection & Request Instructions

Notification of anticoagulant therapy is essential

One (1x) Sodium citrate tubes is required. Do not use 1 ml citrate tubes.

Filled to label marker and mixed by inversion

The patient must not be receiving heparin

Transport to laboratory immediately. (Sample must be double spun and frozen within 4 hours from time of collection).

Tests performed:

INR, APTT, APTT Mix, Silica Clotting Time and Dilute Russell's Viper venom time (LA screen/Confirm).

Notification of anticoagulant therapy is essential

One (1x) Sodium citrate tubes is required. Do not use 1 ml citrate tubes.

Filled to label marker and mixed by inversion

The patient must not be receiving heparin

Unspun sample is stable for up to 4 hours and must be transport to laboratory immediately. (Sample must be double spun, aliquoted and frozen within 4 hours from time of collection).

Tests performed:

INR, APTT, APTT Mix, Silica Clotting Time and Dilute Russell's Viper venom time (LA screen/Confirm).

Processing Instructions

Transport sample to laboratory within 4 hours of collection.

The sample MUST be double spun and frozen within the 4 hour time limit.

Unspun sample is stable for up to 4 hours and must be transport to laboratory immediately. (Sample must be double spun, aliquoted and frozen within 4 hours from time of collection).

External Transport Instructions

Samples can be transported frozen to the laboratory if the facilities are available to double spin and aliquot these sample. This has to be done within 4 hours.

Unspun sample is stable for up to 4 hours and must be transport to laboratory immediately. (Sample must be double spun, aliquoted and frozen within 4 hours from time of collection).

Collection & Request Instructions

Notification of anticoagulant therapy is essential

One (1x) Sodium citrate tubes is required. Do not use 1 ml citrate tubes.

Filled to label marker and mixed by inversion

The patient must not be receiving heparin

Transport to laboratory immediately. (maximum of 4 hours from time of collection).

Tests performed:

INR, APTT, APTT Mix, Silica Clotting Time and Dilute Russell's Viper venom time (LA screen/Confirm).

Notification of anticoagulant therapy is essential

One (1x) Sodium citrate tubes is required. Do not use 1 ml citrate tubes.

Filled to label marker and mixed by inversion

The patient must not be receiving heparin

Transport to laboratory immediately. (Sample must be double spun and frozen within 4 hours from time of collection).

Tests performed:

INR, APTT, APTT Mix, Silica Clotting Time and Dilute Russell's Viper venom time (LA screen/Confirm).

Processing Instructions

Transport sample to laboratory within 4 hours of collection.

The sample MUST be double spun and frozen within the 4 hour time limit.

Date

Field

Changed From

Changed To

Alternative names

CD3 count, CD4 count, CD8 count, CD4:CD8 ratio CD19 count, CD56 count, flow cytometry

Alternative names

CD19 count & CD56 count, CD4 count, CD4 count, CD4:CD8 ratio Lymphocyte subsets include: CD3 count, CD4:CD8 ratio, CD8 count, CD8 count, T subsets include: CD3 count

T subsets include: CD3 count, CD4 count, CD8 count, CD4:CD8 ratio Lymphocyte subsets include: CD3 count, CD4 count, CD8 count, CD4:CD8 ratio, CD19 count & CD56 count Flow Cytometry

Collection & Request Instructions

Sample store at Room temperature. Samples are not stable for more than 24 hours. Hence they must be received by the Flow Cytometry lab at Box Hill within normal business hours Monday to Friday.

Cut-off for outpatient sample collection on Fridays is at 11.30 AM and MUST reach the laboratory before 4.00PM.

Flow cytometric analysis of cells stained by immunofluorescence with a range of antibodies against lymphoid and myeloid markers.

The panel of markers used will be appropriate to the panel selected and the clinical comments supplied, or as arranged by prior consultation.

Sample store at ROOM TEMPERATURE. Samples are not stable for more than 24 hours. Hence they must be received by the Flow Cytometry lab at Box Hill within normal business hours Monday to Friday.

Cut-off for outpatient sample collection on Fridays is at 11.30 AM and MUST reach the laboratory before 4.00PM.

Flow cytometric analysis of cells stained by immunofluorescence with a range of antibodies against lymphoid and myeloid markers.

The panel of markers used will be appropriate to the panel selected and the clinical comments supplied, or as arranged by prior consultation.

Collection & Request Instructions

The samples are not stable for more than 24 hours. Hence all samples must be recieved in the laboratory at Box Hill  Monday to Friday within business hours.

Cutoff for outpatient sample collection on Fridays is at 11.30 AM and MUST reach the laboratory before 4.00PM.

Sample store at Room temperature. Samples are not stable for more than 24 hours. Hence they must be received by the Flow Cytometry lab at Box Hill within normal business hours Monday to Friday.

Cut-off for outpatient sample collection on Fridays is at 11.30 AM and MUST reach the laboratory before 4.00PM.

Flow cytometric analysis of cells stained by immunofluorescence with a range of antibodies against lymphoid and myeloid markers.

The panel of markers used will be appropriate to the panel selected and the clinical comments supplied, or as arranged by prior consultation.

Frequency

Daily

Notes

Used to monitor T-cell numbers

Preferred EDTA sample. Do not spin, store at room temperature.

Samples must be marked 'URGENT', must reach the laboratory as soon as possible. 

All out-of-hours testing requires notification.

Time and date of collection must be recorded on request form.

Whole blood - 1 x EDTA sample, do not spin. Store at room temperature. An FBE must also be requested, and needs a separate EDTA sample.

Assay name

T Cell Subsets

Lymphocyte subsets

Alternative names

CD2, CD3, CD4, CD8

T subsets include: CD3 count, CD4 count, CD8 count, CD4:CD8 ratio Lymphocyte subsets include: CD3 count, CD4 count, CD8 count, CD4:CD8 ratio, CD19 count & CD56 count

Request Group

FLOW

TSUB & LSUB

Processing Instructions

Prefer EDTA sample.

Do not spin, store at room temperature.

Prefer EDTA sample.

Do not spin, store at room temperature.

Notes

Used to monitor T-cell numbers

Preferred EDTA sample. Do not spin, store at room temperature.

Used to monitor T-cell numbers

Preferred EDTA sample. Do not spin, store at room temperature.

Collection & Request Instructions

The samples are not stable for more than 24 hours. Hence all samples must be recieved in the laboratory at Box Hill  Monday to Friday within business hours.

The samples are not stable for more than 24 hours. Hence all samples must be recieved in the laboratory at Box Hill  Monday to Friday within business hours.

Cutoff for outpatient sample collection on Fridays is at 11.30 AM and MUST reach the laboratory before 4.00PM.

Processing Instructions

Prefer EDTA sample.

Do not spin, store at room temperature.

Prefer EDTA sample.

Do not spin, store at room temperature.

Notes

Used to monitor T-cell numbers

Preferred EDTA sample. Do not spin, store at room temperature.

Used to monitor T-cell numbers

Preferred EDTA sample. Do not spin, store at room temperature.

Date

Field

Changed From

Changed To

Laboratory

Microbiology

Biochemistry

Request Group

SMEA

MEA

Frequency

Daily

Twice weekly

Date

Field

Changed From

Changed To

Laboratory

Referred Test

Biochemistry

Collection & Request Instructions

Send ASAP (within 24 hrs, 7 days a week). Contact reference laboratory prior to sending the sample.

Testing performed on an as needs basis. 

External Laboratory

Peter MacCallum Cancer Institute

Method

Hand deliover samples to Biochemistry DO NOT load onto the 8100

Date

Field

Changed From

Changed To

Laboratory

Microbiology

Biochemistry

Request Group

SMUM

MUM

Frequency

Daily

Twice weekly

Date

Field

Changed From

Changed To

Collection & Request Instructions

Note: This is an URGENT send out test.

Laboratory hours are 9am - 3pm, Monday - Friday.

Note: This is an URGENT send out test.

Laboratory hours are 9am - 3pm, Monday - Friday.

Specimen must arrive at Alfred Pathology by 4pm.

External Transport Instructions

Place the specimen in an esky with ice cubes and dispatch immediately.

Place the specimen in an esky with ice cubes and dispatch immediately. Must arrive at Alfred by 4pm.

Collection & Request Instructions

Note: This is an URGENT send out test.

Laboratory hours are 9am - 5pm, Monday - Friday.

Note: This is an URGENT send out test.

Laboratory hours are 9am - 3pm, Monday - Friday.

Date

Field

Changed From

Changed To

Collection & Request Instructions

Separate specimen required.

Cutoff for outpatient sample collection on Fridays is at 11.30 AM and MUST reach the laboratory before 4.00PM.

Whole blood - 1 x EDTA sample, do not spin. Store at room temperature. An FBE and BLOOD FILM must also be requested,  and needs a separate EDTA sample.

Cutoff for outpatient sample collection on Fridays is at 11.30 AM and MUST reach the laboratory before 4.00PM.

Alternative names

CD 59, PNH flow cytometry, PNH screen

CD59, PNH screen flow cytometry, FLEAR

Alternative names

CD 59, PNH screen

CD 59, PNH screen, PNH flow cytometry

Request Group

FLOW

PNHS

Collection & Request Instructions

Separate specimen required.

Separate specimen required.

Cutoff for outpatient sample collection on Fridays is at 11.30 AM and MUST reach the laboratory before 4.00PM.

Date

Field

Changed From

Changed To

Method

All PCT requests must be discussed with the Chemical Pathologist upon receipt in lab, regardless of time of day or day of week. If urgent/Taxi requiring is determined, result must be phoned to the clinician upon receipt - High Risk Result.

Method

All PCT requests must be discussed with the Chemical Pathologist upon receipt in lab, regardless of time of day or day of week. If urgent/Taxi requiring is determined, result must be phoned to the clinician upon receipt - High Risk Result.

Laboratory

Biochemistry

Referred Test

Volume (Adults)

0.5 mL

External Laboratory

Royal Childrens Hospital

Date

Field

Changed From

Changed To

Collection & Request Instructions

Filled to label marker and mixed by inversion.

Patient Information - Warfarin Management

Filled to label marker and mixed by inversion.

Patient Information - Warfarin Management-English
Patient Information - Warfarin Management-Greek
Patient Information - Warfarin Management-Simplified Chinese

Date

Field

Changed From

Changed To

External Laboratory

Victorian Infectious Diseases Reference Laboratory (V.I.D.R.L)

Date

Field

Changed From

Changed To

Collection & Request Instructions

Minimum volume of 4 ml required - this specimen cannot be used for any other purpose.

A completed "ARCBS Red Cell Reference Form" must accompany each sample sent for analysis.

This form, which can be completed online and subsequently printed to be sent with the sample can be found by clicking here 

Minimum volume of 4 ml required - this specimen cannot be used for any other purpose.

A completed "ARCBS Red Cell Reference Form" must accompany each sample sent for analysis.

This form, which can be completed online and subsequently printed to be sent with the sample can be found by clicking here 

Processing Instructions

Specimen will arrive in department at RT.  Please keep at 4 degrees prior to transport.

Specimen will arrive in department at RT.  Please keep at 4 degrees prior to transport.

External Transport Instructions

Transport at 4 degrees

Transport at 4 degrees

Date

Field

Changed From

Changed To

Method

The following specimens will be referred for testing-

Sputum, Bronchial Washings, Bronchoalveolar Lavages - VIDRL

Swab specimens for Respiratory Multiplex PCR will be referred to Monash Molecular Laboratory

The following specimens will be referred for testing-

Sputum, Bronchial Washings, Bronchoalveolar Lavages - VIDRL

As of Feb 2023 swab specimens for Respiratory Multiplex PCR will be tested in-house.

Preferred Specimen Type

Nasopharyngeal swab
Nasopharyngeal Aspirate

Nasopharyngeal swab
NTS - nose & throat swab
Nasopharyngeal Aspirate

Optional Specimen Type

Sputum
Bronchial Washings
Broncho-alveolar Lavage

Preferred Container Type

Viral Transport Media- Nasopharyngeal
Respiratory aspiration pot

Viral Transport Media- Nasopharyngeal
Respiratory aspiration pot
respiratory PCR requirements

Collection & Request Instructions

Please note these swabs are available from stores.

Search for: VIRAL TRANSPORT MEDIA - NASOPHARYNGEAL

Pathology do not keep a stock of these swabs. Emergency departments and wards should manage a supply of these swabs according to demand.

Please note these swabs are available from stores.

Pathology do not keep a stock of these swabs. Emergency departments and wards should manage a supply of these swabs according to demand.

Method

All tests for respiratory virus PCR are considered urgent and must be delivered to the laboratory ASAP.

ICU/Oncology patients testing negative for Flu A/B and RSV  will be referred to Monash for respiratory virus panel. This includes Influenza A virus, Influenza B virus, Respiratory Syncitial Virus, Picornavirus (RV/EV), Parainfluenza viruses 1,2 and 3, Adenovirus, Human Metapneumovirus, Bordetella pertussis, Bordetella parapertussis, Mycoplasma pneumoniae, Legionella species, Chlamydiaceae.

The following specimens will be referred for testing-

Sputum, Bronchial Washings, Bronchoalveolar Lavages

Specimens for Respiratory Multiplex PCR will be referred to Monash Molecular Laboratory

Contact Monash Pathology 9594 4532 or if no answer try Bacteriology 9594 4565 as there is someone rostered 24/7

The following specimens will be referred for testing-

Sputum, Bronchial Washings, Bronchoalveolar Lavages - VIDRL

Swab specimens for Respiratory Multiplex PCR will be referred to Monash Molecular Laboratory

Notes

This PCR will detect and identify: Influenza A virus, Influenza B virus and Respiratory syncytial virus (RSV).

Transport at room temperature to the Laboratory

Testing is performed at Box Hill, Angliss, and Maroondah.

Samples received after hours may have a longer TAT due to other urgent critical pathology testing occurring.

This PCR will detect and identify: Influenza A virus, Influenza B virus and Respiratory syncytial virus (RSV).

Transport at room temperature to the Laboratory

Testing is performed at Box Hill, Angliss, and Maroondah.

DO NOT send samples in the Pathology Chute

For Rapid testing from Emergency Department - swab must be accompanied by signed Rapid Test Authorisation Form.

DO NOT call the laboratory for results/enquiries. Inpatient tests will be prioritised and results available on EMR ASAP.

Sputum samples are referred to an external laboratory - results will be delayed.

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Preferred Container Type

Viral Transport Media - Nasopharyngeal

Viral Transport Media - Nasopharyngeal
respiratory PCR requirements
Viral Transport Media- Nasopharyngeal

Preferred Specimen Type

NTS - nose & throat swab
Swab

NTS - nose & throat swab
Respiratory samples

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External Laboratory

Victorian Infectious Diseases Reference Laboratory (V.I.D.R.L)

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Alternative names

RICOF

RICOF, vWF:Activity, vWF:Act

Laboratory

Referred Test

Coagulation

External Laboratory

Austin Pathology

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Collection & Request Instructions

Scabies collection instructions

Preparation

Wash the area of skin to be scraped with an alcohol swab.

Remove any ointment or residual applications using soap and water.

Disinfect the area with a skin cleansing alcohol wipe and allow it to dry.

Abrade the infested area with a sterile scalpel to the depth of pinpoint bleeding.

Transfer skin scrapings to a sterile yellow top pot for transport to the laboratory. Do NOT include the scalpel – dispose of in sharps bin

Label pot with patient name, UR No and DOB, date of collection, site of collection

Preparation

Wash the area of skin to be scraped with an alcohol swab.

Remove any ointment or residual applications using soap and water.

Disinfect the area with a skin cleansing alcohol wipe and allow it to dry.

Abrade the infested area with a sterile scalpel to the depth of pinpoint bleeding.

Transfer skin scrapings to a sterile yellow top pot for transport to the laboratory. Do NOT include the scalpel – dispose of in sharps bin

Label pot with patient name, UR No and DOB, date of collection, site of collection

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Preferred Container Type

Lithium heparin no gel
EDTA

See Instructions

Collection & Request Instructions

Collect 1 x 4 ml Lithium Heparin tube and

1 x 4ml EDTA tube.

FBE must be requested with this test as a RCC is required for calculation of results

DO NOT SPIN.

Keep at Room Temp.

Collect 1 x 4 ml Lithium Heparin tube and

2 x 4ml EDTA tube.

FBE must be requested with this test as a RCC is required for calculation of results

DO NOT SPIN.

Keep at Room Temp.

Collection & Request Instructions

Collect 1 x 4 ml Lithium Heparin tube and 1 x 4ml EDTA tube.

FBE must be requested with this test as a RCC is required for calculation of results

DO NOT SPIN.

Keep at Room Temp.

Collect 1 x 4 ml Lithium Heparin tube and

1 x 4ml EDTA tube.

FBE must be requested with this test as a RCC is required for calculation of results

DO NOT SPIN.

Keep at Room Temp.

Request Group

TMETAB

Preferred Container Type

EDTA

Lithium heparin no gel
EDTA

Collection & Request Instructions

Transport specimen at Room Temperature to lab

Collection & Request Instructions

Collect 2x4ml EDTA blood. DO NOT SPIN

Keep at 4 deg C, do not freeze.

The metabolites are stable for upto 4 days at 4 degress.

Collect 1 x 4 ml Lithium Heparin tube and 1 x 4ml EDTA tube.

FBE must be requested with this test as a RCC is required for calculation of results

DO NOT SPIN.

Keep at Room Temp.

Processing Instructions

Keep samples at Room Temperature.

Do Not Spin. Whole Blood required

External Laboratory

St Vincents Hospital

Frequency

Weekly

Notes

Imuran is a trade name for azathioprine

The Thiopurine metabolites (6-TGN & 6-MMP) are used as a means of estimating the toxicity and efficacy of azathioprine treatment.

This test is performed by the Gastroenerology department. ( Phone No.9895 4632 )

The cost for the test is not covered by Medicare. 

Eastern Health patients:6TGN 6MMP Thiopurine Metabolites Testing $75

All other patients: 6TGN 6MMP Thiopurine Metabolites Testing $145

Imuran is a trade name for azathioprine

The Thiopurine metabolites (6-TGN & 6-MMP) are used as a means of estimating the toxicity and efficacy of azathioprine treatment.

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Laboratory

Unassigned

Anatomical Pathology

External Transport Instructions

https://pathology.easternhealth.org.au/handbook/downloads/anatomical-pathology/Tissue%20Release%20Consent%20Form.doc

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Request Group

STOX

TOX

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Processing Instructions

The serum must be aliquoted and frozen. Transthyretin is only stable for 3 days at 4-8C .

The serum must be aliquoted and frozen. Transthyretin is only stable for 3 days at 4-8C .

External Laboratory

Monash Pathology

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Alternative names

enteric fever, Typhoid fever

enteric fever, typhoid fever

Preferred Container Type

Blood Culture

Blood Culture (Adult)
Blood Culture (Paediatric)

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External Laboratory

Australian Clinical Labs (formerly Healthscope)

St Vincents Hospital

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External Laboratory

Australian Clinical Labs (formerly Healthscope)

St Vincents Hospital

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Volume (Adults)

2 mL

1 mL

Minimum/Paediatric Volume

1 mL

Processing Instructions

Spin, separate, wrap in foil and freeze ASAP.

Transport frozen. Contact Gribbles to arrange courier.

Spin, separate, wrap in foil and freeze ASAP.

Transport frozen. 

External Laboratory

Australian Clinical Labs (formerly Healthscope)

Alfred Hospital

Frequency

Weekly

Fortnightly

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Laboratory

Referred Test

Biochemistry

Collection & Request Instructions

Please note : to avoid sample deterioration, DO NOT send CSF specimens via the vacuum chute system.

Please quickly check that the tube is labelled underneath the foil before referring.

THERE MUST BE A MINIMUM OF 0.6 mL OF CSF OR THE TEST CANNOT BE PERFORMED

Please note : to avoid sample deterioration, DO NOT send CSF specimens via the vacuum chute system.

THERE MUST BE A MINIMUM OF 0.6 mL OF CSF OR THE TEST CANNOT BE PERFORMED

Processing Instructions

CSF xanthochromia checklist

Sample collected >12hrs from onset symptoms   Preferable

      4^th CSF sample    Essential

Sample light protected immediately   Essential

Sample transported manually to lab   Essential

Serum sample for bilirubin & albumin   Preferable

Slip with clinical notes and contact details   Essential

CSF xanthochromia checklist

Sample collected >12hrs from onset symptoms   Preferable

      4^th CSF sample    Essential

Sample light protected immediately   Essential

Sample transported manually to lab   Essential

Serum sample for bilirubin & protein- essemtial

Slip with clinical notes and contact details   Essential

External Laboratory

Austin Pathology

External Transport Instructions

Send via a Taxi ASAP. Ring Austin Pathology on 94963100.

MH and AH send via taxi ASAP and notify BH laboraotry.

Frequency

Daily

As required

Method

Please quickly check that the tube is labelled underneath the foil before passing to Scientist.

The specimen designated for spectrophotometry should be centrifuged at .2000 rpm for 5 min as soon as possible after receipt in the laboratory. The supernatant must be stored in the dark at 4⁰ C until analysis.

Notes

This test is specialised and will only be performed on CSF's when:

CT scan is negative

Four tubes must be collected and the container of the 4th tube must be wrapped in foil (please Label specimen before wrapping in foil)

This test is specialised and will only be performed on CSF's when:

CT scan is negative

Four tubes must be collected and the container of the 4th tube must be wrapped in foil (please Label specimen before wrapping in foil)

A white top tube must be collected for simulataneous measurement of serum Bilirubin and serum Protein (or add on to existing bloods within 6 hours of CSF collection).