New search...
Recent changes for all departments
Select department:
Recent changes for Adrenocorticotrophic Hormone
Recent changes for Adrenocorticotrophic Hormone |
Date | Field | Changed From | Changed To |
2nd August 2022 | Notes | Consultation with Endocrinologist suggested
Collect in the morning between 0800 and 1000h.
1 x 4mL EDTA tube on ice.
Send to laboratory for immediate centrifugation. Spin, separate and freeze plasma. Transport frozen.
| Consultation with Endocrinologist suggested
Collect 1 x 4mL EDTA tube on ice.
Send to laboratory for immediate centrifugation. Spin, separate and freeze plasma.
|
Recent changes for Alpha-1 Antitrypsin - Quantitation
Recent changes for Alpha-1 Antitrypsin - Quantitation |
Date | Field | Changed From | Changed To |
13th September 2022 | External Laboratory | St Vincents Hospital | |
Recent changes for Anti-diuretic hormone
Recent changes for Anti-diuretic hormone |
Date | Field | Changed From | Changed To |
7th October 2021 | Alternative names | ADH | |
| Request Group | ADH | |
| Preferred Specimen Type | Plasma | Serum |
| Optional Specimen Type | | Plasma |
| Preferred Container Type | EDTA | Serum tube with gel |
| Optional Container Type | | EDTA Lithium heparin no gel |
| Collection & Request Instructions | | Samples should be fasting. |
| Processing Instructions |
Spin, separate and freeze plasma.
Packed by certified personnel.
Sent frozen.
Specimen Reception, Level 6 (E Block), RPA, Missenden Road, Camperdown , NSW 2050
Tel: 02-95158279 | Spin, separate and freeze serum/plasma
Packed by certified personnel.
Sent frozen.
Specimen Reception, Level 6 (E Block), RPA, Missenden Road, Camperdown , NSW 2050
Tel: 02-95158279 |
Recent changes for Anti-Xa Assay
Recent changes for Anti-Xa Assay |
Date | Field | Changed From | Changed To |
18th May 2021 | Request Group | 10A: Clexane, 10AH:Plasma Heparin, APIX: Apixaban, RIVX: Rivaroxaban | 10A: Clexane, 10AH:Plasma Heparin, APIX: Apixaban, RIVX: Rivaroxaban, FONX: Fondaparinux |
18th May 2021 | Request Group | 10A | 10A: Clexane, 10AH:Plasma Heparin, APIX: Apixaban, RIVX: Rivaroxaban |
Recent changes for Azathioprine Metabolite Testing
Recent changes for Azathioprine Metabolite Testing |
Date | Field | Changed From | Changed To |
23rd August 2022 | Processing Instructions |
Place the samples and request slip in fridge (M43) in CSR. Do not enter in the computer.
Eastern Health Clinical School
Level 2, 5 Arnold Street,
Box Hill Vic 3128
Ph: 61 3 9091 8827
Fax: 61 3 9899 9137
E-mail: ehcs@monash.edu | |
Recent changes for Bile Acids
Recent changes for Bile Acids |
Date | Field | Changed From | Changed To |
23rd May 2022 | Laboratory | Referred Test | Biochemistry |
| Preferred Container Type | Plain Tube with gel | Serum tube with gel |
| Collection & Request Instructions | Fasting or Non Fasting sample.
Note: This test is ONLY performed on pregnant women.
| Fasting.
Note: This test is ONLY performed on pregnant women.
|
| Processing Instructions | Spin, separate ASAP. | |
| External Laboratory | Royal Childrens Hospital | |
| Frequency | Weekly | Twice weekly |
| Method | Store and transport at 4 C. | |
Recent changes for Blood Cultures - Adult
Recent changes for Blood Cultures - Adult |
Date | Field | Changed From | Changed To |
9th August 2021 | Processing Instructions | Bottles should be loaded on to the Bactec analyser as soon as possible. If entry is delayed, store bottles at room temperature. | Bottles should be loaded on to the Bactec FX analyser as soon as possible. If entry is delayed, store bottles at room temperature. |
9th August 2021 | Processing Instructions | Bottles should be loaded on to the Virtuo analyser as soon as possible. If entry is delayed, store bottles at room temperature. | Bottles should be loaded on to the Bactec analyser as soon as possible. If entry is delayed, store bottles at room temperature. |
25th May 2021 | Volume (Adults) | 10 mL | 8 mL |
| Preferred Container Type | Blood Culture | Blood Culture (Adult) |
Recent changes for Blood Cultures - Children and Neonates
Recent changes for Blood Cultures - Children and Neonates |
Date | Field | Changed From | Changed To |
9th August 2021 | External Transport Instructions | Load as soon as possible onto the Virtuo Analyzer. Store at room temperature until loaded | Load as soon as possible onto the Bactec FX Analyzer. Store at room temperature until loaded |
25th May 2021 | Volume (Adults) | 4 mL | |
| Minimum/Paediatric Volume | | 1 mL |
| Preferred Container Type | Blood Culture Paediatric | Blood Culture (Paediatric) |
| Collection & Request Instructions | Add 0.5 - 4mL of blood to a single aerobic paediatric bottle.
For patients with suspected bacterial endocarditis, 2 to 3 sets taken at different times should be sufficient to establish a diagnosis.
Collect blood cultures 20 minutes apart. If patient is too ill to wait 20 minutes, prior to administering antibiotics, collect the first set from one site and the second from a different site.
Please provide clinical details. Extended incubation is required for query endocarditis, brucellosis or fungal infection
Transport at room temperature
| Add 1 - 3mL of blood to a single aerobic paediatric bottle.
For patients with suspected bacterial endocarditis, 2 to 3 sets taken at different times should be sufficient to establish a diagnosis.
If multiple sets required, collect blood cultures 20 minutes apart. If patient is too ill to wait 20 minutes, prior to administering antibiotics, collect the first set from one site and the second from a different site.
Please provide clinical details. Extended incubation is required for query endocarditis, brucellosis or fungal infection
Transport at room temperature
|
Recent changes for Bone Marrow Examination
Recent changes for Bone Marrow Examination |
Date | Field | Changed From | Changed To |
25th March 2022 | Laboratory | Unassigned | Haematology |
Recent changes for Bone Specific Alkaline Phosphatase
Recent changes for Bone Specific Alkaline Phosphatase |
Date | Field | Changed From | Changed To |
15th June 2021 | Assay name | Bone Specific Alkiline Phosphatase | Bone Specific Alkaline Phosphatase |
| External Laboratory | Monash Pathology | Royal Melbourne Hospital |
Recent changes for BRCA1/BRCA2
Recent changes for BRCA1/BRCA2 |
Date | Field | Changed From | Changed To |
17th June 2021 | Request Group | | CHROM |
| Collection & Request Instructions | | Send to Peter Mac on next available courier. Stable at room temperature for a maximum of 2 weeks. |
| Processing Instructions | | Send to Peter Mac on next available courier. Stable at room temperature for a maximum of 2 weeks. |
| External Transport Instructions | Room Temperature | Room Temperature
Send to Peter Mac on next available courier. Stable at room temperature for a maximum of 2 weeks. |
| Method | | Send to Peter Mac on next available courier. Stable at room temperature for a maximum of 2 weeks. |
Recent changes for Cadmium
Recent changes for Cadmium |
Date | Field | Changed From | Changed To |
13th September 2022 | Notes | Transport specimen at Room Temperature to lab
Sample haemolysis may make this test unreportable
| Transport specimen at Room Temperature to lab
Sample haemolysis may make this test unreportable
Send as whole blood
|
Recent changes for Caeruloplasmin
Recent changes for Caeruloplasmin |
Date | Field | Changed From | Changed To |
13th September 2022 | External Laboratory | Austin Pathology | |
Recent changes for CASA
Recent changes for CASA |
Date | Field | Changed From | Changed To |
17th February 2022 | External Laboratory | Royal Brisbane & Womens Hospital | |
| External Transport Instructions | Transport frozen.
Packed by certified personnel. | Transport frozen.
Packed by certified personnel. |
| Notes | In combination with the tumour marker CA-125, CASA is used for differentiating between benign gynaecological disease and ovarian carcinoma. Please note that this test has changed referral laboratories as at 18/1/2011.
Please contact Chemical Pathologist before requesting this test. | In combination with the tumour marker CA-125, CASA is used for differentiating between benign gynaecological disease and ovarian carcinoma. Please note that this test has changed referral laboratories as at 18/1/2011. This test is no longer available in Australia
Please contact Chemical Pathologist before requesting this test.
|
Recent changes for Cerebrospinal Fluid - Biochemistry
Recent changes for Cerebrospinal Fluid - Biochemistry |
Date | Field | Changed From | Changed To |
27th April 2021 | Collection & Request Instructions | Please note : to avoid sample deterioration, DO NOT send CSF specimens via the vacuum chute system.
Depending on the tests requested, 3-4 separate tubes will need to be provided. Note that the tubes are numbered so that order of collection can be identified – this order should be carefully followed, so that the optimal sample can be used for the requested tests. It is also important to consider the tests that are required, as this will determine how much CSF is needed in each tube. See the matrix below for details.
TUBE 1 is used for Cytology.
TUBE 2 is used for Glucose, Protein and Flow Cytometry.
(centrifuged in the lab – the supernatant is used for Biochemistry analysis (glucose, protein) and the cellular pellet will be used for flow cytometry if required).
TUBE 3 is for Microbiological analysis (cell count, Gram stain and culture) and any further infectious testing (e.g. viral PCR, mycobacterial PCR and culture, cryptococcal antigen).
TUBE 4 will be required only for;
(a) Creutzfeld-Jacob Disease (CJD) testing OR
(b) Xanthochromia analysis (wrap the tube in foil to prevent sample deterioration due to light).
TESTS REQUIRED
|
MINIMUM REQUIRED VOLUME (mL)
|
TUBE 1
|
TUBE 2
|
TUBE 3
|
TUBE 4
|
CYTOLOGY. CHEMISTRY, MICRO/CULTURE
|
1.0-2.0
|
1.0
|
1.0-2.0
|
Nil
|
ADD FLOW CYTOMETRY
|
|
add 5.0-7.0
|
|
|
ADD OTHER INFECTIOUS MARKERS
|
|
|
add 2.0- 5.0-
|
|
ADD MYCOBACTERIAL CULTURE
|
|
|
Add 10.00
|
|
ADD CJD
|
|
|
|
1.0
|
ADD XANTHOCHROMIA
|
|
|
|
1.0 (foil)
|
Note, the ability to add further tests to a previously collected sample is dependent upon the volume provided. Also, many tests (flow cytometry, cytology) can only be performed on fresh specimens.
Other specialised testing (e.g. oligoclonal bands) will be performed using the supplied samples.
Xanthochromia and CJD analysis cannot be performed on a blood-stained sample.
CSF Information - Laboratory Processing | Depending on the tests requested, 3-4 separate tubes will need to be provided. Note that the tubes are numbered so that order of collection can be identified – this order should be carefully followed, so that the optimal sample can be used for the requested tests. It is also important to consider the tests that are required, as this will determine how much CSF is needed in each tube. See the matrix below for details.
TUBE 1 is used for Biochemistry and Flow Cytometry. (Centrifuged in the lab – the supernatant is used for Biochemistry analysis (glucose, protein) and the cellular pellet will be used for flow cytometry if required). If tube 3 is bloodstained, take an aliquot for microbiology.
TUBE 2 is used for Cytology
TUBE 3 is for Microbiological analysis (cell count, Gram stain and culture) and any further infectious testing (e.g. viral PCR, mycobacterial PCR and culture, cryptococcal antigen).
TUBE 4 will be required only for;
(a) Creutzfeld-Jacob Disease (CJD) testing OR
(b) Xanthochromia analysis (wrap the tube in foil to prevent sample deterioration due to light).
TESTS REQUIRED
|
MINIMUM REQUIRED VOLUME (mL)
|
TUBE 1
|
TUBE 2
|
TUBE 3
|
TUBE 4
|
CHEMISTRY
|
1.0
|
|
|
|
ADD FLOW CYTOMETRY
|
Minimum 5.0-7.0
|
|
|
|
CYTOLOGY
|
|
1.0-3.0
|
|
|
MICROBIOLOGY
|
|
|
1.0-2.0
|
|
ADD OTHER INFECTIOUS MARKERS
|
|
|
add 2.0- 5.0-
|
|
ADD MYCOBACTERIAL CULTURE
|
|
|
Add 6.0-10.00
|
|
ADD CJD
|
|
|
|
1.0
|
ADD XANTHOCHROMIA
|
|
|
|
1.0 (foil)
|
Note, the ability to add further tests to a previously collected sample is dependent upon the volume provided. Also, many tests (flow cytometry, cytology) can only be performed on fresh specimens.
Other specialised testing (e.g. oligoclonal bands) will be performed using the supplied samples.
Xanthochromia and CJD analysis cannot be performed on a blood-stained sample.
|
Recent changes for Cerebrospinal Fluid - Cytology
Recent changes for Cerebrospinal Fluid - Cytology |
Date | Field | Changed From | Changed To |
27th April 2021 | Collection & Request Instructions | The sample with the least amount of blood is prefered for Cytology.
CSF SPECIMEN INFORMATION – LABORATORY PROCESSING
Depending on the tests requested, 3-4 separate tubes will need to be provided. Note that the tubes are numbered so that order of collection can be identified – this order should be carefully followed, so that the optimal sample can be used for the requested tests. It is also important to consider the tests that are required, as this will determine how much CSF is needed in each tube. See the matrix below for details.
TUBE 1 is used for Cytology.
TUBE 2 is used for Glucose, Protein and Flow Cytometry.
(centrifuged in the lab – the supernatant is used for Biochemistry analysis (glucose, protein) and the cellular pellet will be used for flow cytometry if required).
TUBE 3 is for Microbiological analysis (cell count, Gram stain and culture) and any further infectious testing (e.g. viral PCR, mycobacterial PCR and culture, cryptococcal antigen).
TUBE 4 will be required only for;
(a) Creutzfeld-Jacob Disease (CJD) testing OR
(b) Xanthochromia analysis (wrap the tube in foil to prevent sample deterioration due to light).
TESTS REQUIRED
|
MINIMUM REQUIRED VOLUME (mL)
|
TUBE 1
|
TUBE 2
|
TUBE 3
|
TUBE 4
|
CYTOLOGY. CHEMISTRY, MICRO/CULTURE
|
1.0-2.0
|
1.0
|
1.0-2.0
|
Nil
|
ADD FLOW CYTOMETRY
|
|
add 5.0-7.0
|
|
|
ADD OTHER INFECTIOUS MARKERS
|
|
|
add 2.0- 5.0-
|
|
ADD MYCOBACTERIAL CULTURE
|
|
|
Add 10.00
|
|
ADD CJD
|
|
|
|
1.0
|
ADD XANTHOCHROMIA
|
|
|
|
1.0 (foil)
|
Note, the ability to add further tests to a previously collected sample is dependent upon the volume provided. Also, many tests (flow cytometry, cytology) can only be performed on fresh specimens.
Other specialised testing (e.g. oligoclonal bands) will be performed using the supplied samples.
Xanthochromia and CJD analysis cannot be performed on a blood-stained sample. | The sample with the least amount of blood is prefered for Cytology.
CSF SPECIMEN INFORMATION – LABORATORY PROCESSING
Depending on the tests requested, 3-4 separate tubes will need to be provided. Note that the tubes are numbered so that order of collection can be identified – this order should be carefully followed, so that the optimal sample can be used for the requested tests. It is also important to consider the tests that are required, as this will determine how much CSF is needed in each tube. See the matrix below for details.
TUBE 1 is used for Biochemistry and Flow Cytometry. (Centrifuged in the lab – the supernatant is used for Biochemistry analysis (glucose, protein) and the cellular pellet will be used for flow cytometry if required). If tube 3 is bloodstained, take an aliquot for microbiology.
TUBE 2 is used for Cytology
TUBE 3 is for Microbiological analysis (cell count, Gram stain and culture) and any further infectious testing (e.g. viral PCR, mycobacterial PCR and culture, cryptococcal antigen).
TUBE 4 will be required only for;
(a) Creutzfeld-Jacob Disease (CJD) testing OR
(b) Xanthochromia analysis (wrap the tube in foil to prevent sample deterioration due to light).
TESTS REQUIRED
|
MINIMUM REQUIRED VOLUME (mL)
|
TUBE 1
|
TUBE 2
|
TUBE 3
|
TUBE 4
|
CHEMISTRY
|
1.0
|
|
|
|
ADD FLOW CYTOMETRY
|
Minimum 5.0-7.0
|
|
|
|
CYTOLOGY
|
|
1.0-3.0
|
|
|
MICROBIOLOGY
|
|
|
1.0-2.0
|
|
ADD OTHER INFECTIOUS MARKERS
|
|
|
add 2.0- 5.0-
|
|
ADD MYCOBACTERIAL CULTURE
|
|
|
Add 6.0-10.00
|
|
ADD CJD
|
|
|
|
1.0
|
ADD XANTHOCHROMIA
|
|
|
|
1.0 (foil)
|
Note, the ability to add further tests to a previously collected sample is dependent upon the volume provided. Also, many tests (flow cytometry, cytology) can only be performed on fresh specimens.
Other specialised testing (e.g. oligoclonal bands) will be performed using the supplied samples.
Xanthochromia and CJD analysis cannot be performed on a blood-stained sample.
|
| Method | CSF sample processing:
If Cytology/Flow Cytometry requested : Tube 1-Biochemistry, Tube 2- Cytology/Flow Cytometry, Tube 3-Microbiology
If Cytology/Flow Cytometry not requested: Tube 1 and 3-Microbiology, Tube 2-Biochemistry
Any confusion between the terms cell count and cytology could be cleared up when the cell count is rung through to the requesting doctor by the scientist. At this point it could be determined whether cytology is a genuine request or not (all to be documented in F8 notes).
| |
Recent changes for Cerebrospinal Fluid - microscopy and culture
Recent changes for Cerebrospinal Fluid - microscopy and culture |
Date | Field | Changed From | Changed To |
27th April 2021 | Notes | CSF SPECIMEN INFORMATION – LABORATORY PROCESSING
Depending on the tests requested, 3-4 separate tubes will need to be provided. Note that the tubes are numbered so that order of collection can be identified – this order should be carefully followed, so that the optimal sample can be used for the requested tests. It is also important to consider the tests that are required, as this will determine how much CSF is needed in each tube. See the matrix below for details.
TUBE 1 is used for Cytology.
TUBE 2 is used for Glucose, Protein and Flow Cytometry.
(centrifuged in the lab – the supernatant is used for Biochemistry analysis (glucose, protein) and the cellular pellet will be used for flow cytometry if required).
TUBE 3 is for Microbiological analysis (cell count, Gram stain and culture) and any further infectious testing (e.g. viral PCR, mycobacterial PCR and culture, cryptococcal antigen).
TUBE 4 will be required only for;
(a) Creutzfeld-Jacob Disease (CJD) testing OR
(b) Xanthochromia analysis (wrap the tube in foil to prevent sample deterioration due to light).
TESTS REQUIRED
|
MINIMUM REQUIRED VOLUME (mL)
|
TUBE 1
|
TUBE 2
|
TUBE 3
|
TUBE 4
|
CYTOLOGY. CHEMISTRY, MICRO/CULTURE
|
1.0-2.0
|
1.0
|
1.0-2.0
|
Nil
|
ADD FLOW CYTOMETRY
|
|
add 5.0-7.0
|
|
|
ADD OTHER INFECTIOUS MARKERS
|
|
|
add 2.0- 5.0-
|
|
ADD MYCOBACTERIAL CULTURE
|
|
|
Add 10.00
|
|
ADD CJD
|
|
|
|
1.0
|
ADD XANTHOCHROMIA
|
|
|
|
1.0 (foil)
|
Note, the ability to add further tests to a previously collected sample is dependent upon the volume provided. Also, many tests (flow cytometry, cytology) can only be performed on fresh specimens.
Other specialised testing (e.g. oligoclonal bands) will be performed using the supplied samples.
Xanthochromia and CJD analysis cannot be performed on a blood-stained sample.
Special tests available on request:
PCR for viruses
Acid-fast stain and/or culture for Mycobacteria
Cryptococcal antigen
Serological test for Syphilis (must be paired with 5mL serum sample or test will not be performed)
Viral Serology, if CSF is accompanied by serum sample
Flow Cytometry - requires a 4th tube
The turnaround time for CSF microscopy are 30-40 minutes.
The turnaround time for CSF culture are 3-5 days. | Depending on the tests requested, 3-4 separate tubes will need to be provided. Note that the tubes are numbered so that order of collection can be identified – this order should be carefully followed, so that the optimal sample can be used for the requested tests. It is also important to consider the tests that are required, as this will determine how much CSF is needed in each tube. See the matrix below for details.
TUBE 1 is used for Biochemistry and Flow Cytometry. (Centrifuged in the lab – the supernatant is used for Biochemistry analysis (glucose, protein) and the cellular pellet will be used for flow cytometry if required). If tube 3 is bloodstained, take an aliquot for microbiology.
TUBE 2 is used for Cytology
TUBE 3 is for Microbiological analysis (cell count, Gram stain and culture) and any further infectious testing (e.g. viral PCR, mycobacterial PCR and culture, cryptococcal antigen).
TUBE 4 will be required only for;
(a) Creutzfeld-Jacob Disease (CJD) testing OR
(b) Xanthochromia analysis (wrap the tube in foil to prevent sample deterioration due to light).
TESTS REQUIRED
|
MINIMUM REQUIRED VOLUME (mL)
|
TUBE 1
|
TUBE 2
|
TUBE 3
|
TUBE 4
|
CHEMISTRY
|
1.0
|
|
|
|
ADD FLOW CYTOMETRY
|
Minimum 5.0-7.0
|
|
|
|
CYTOLOGY
|
|
1.0-3.0
|
|
|
MICROBIOLOGY
|
|
|
1.0-2.0
|
|
ADD OTHER INFECTIOUS MARKERS
|
|
|
add 2.0- 5.0-
|
|
ADD MYCOBACTERIAL CULTURE
|
|
|
Add 6.0-10.00
|
|
ADD CJD
|
|
|
|
1.0
|
ADD XANTHOCHROMIA
|
|
|
|
1.0 (foil)
|
Note, the ability to add further tests to a previously collected sample is dependent upon the volume provided. Also, many tests (flow cytometry, cytology) can only be performed on fresh specimens.
Other specialised testing (e.g. oligoclonal bands) will be performed using the supplied samples.
Xanthochromia and CJD analysis cannot be performed on a blood-stained sample. |
Recent changes for Cerebrospinal Fluid Specimen Collection
Recent changes for Cerebrospinal Fluid Specimen Collection |
Date | Field | Changed From | Changed To |
27th April 2021 | Notes | Depending on the tests requested, 3-4 separate tubes will need to be provided. Note that the tubes are numbered so that order of collection can be identified – this order should be carefully followed, so that the optimal sample can be used for the requested tests. It is also important to consider the tests that are required, as this will determine how much CSF is needed in each tube. See the matrix below for details.
TUBE 1 is used for Biochemistry and Flow Cytometry. (Centrifuged in the lab – the supernatant is used for Biochemistry analysis (glucose, protein) and the cellular pellet will be used for flow cytometry if required). If tube 3 is bloodstained, take an aliquot for microbiology.
TUBE 2 is used for Cytology
TUBE 3 is for Microbiological analysis (cell count, Gram stain and culture) and any further infectious testing (e.g. viral PCR, mycobacterial PCR and culture, cryptococcal antigen).
TUBE 4 will be required only for;
(a) Creutzfeld-Jacob Disease (CJD) testing OR
(b) Xanthochromia analysis (wrap the tube in foil to prevent sample deterioration due to light).
TESTS REQUIRED
|
MINIMUM REQUIRED VOLUME (mL)
|
TUBE 1
|
TUBE 2
|
TUBE 3
|
TUBE 4
|
CHEMISTRY
|
1.0
|
|
|
|
ADD FLOW CYTOMETRY
|
Minimum 5.0-7.0
|
|
|
|
CYTOLOGY
|
|
1.0-3.0
|
|
|
MICROBIOLOGY
|
|
|
1.0-2.0
|
|
ADD OTHER INFECTIOUS MARKERS
|
|
|
add 2.0- 5.0-
|
|
ADD MYCOBACTERIAL CULTURE
|
|
|
Add 6.0-10.00
|
|
ADD CJD
|
|
|
|
1.0
|
ADD XANTHOCHROMIA
|
|
|
|
1.0 (foil)
|
Note, the ability to add further tests to a previously collected sample is dependent upon the volume provided. Also, many tests (flow cytometry, cytology) can only be performed on fresh specimens.
Other specialised testing (e.g. oligoclonal bands) will be performed using the supplied samples.
Xanthochromia and CJD analysis cannot be performed on a blood-stained sample.
| Depending on the tests requested, 3-4 separate tubes will need to be provided. Note that the tubes are numbered so that order of collection can be identified – this order should be carefully followed, so that the optimal sample can be used for the requested tests. It is also important to consider the tests that are required, as this will determine how much CSF is needed in each tube. See the matrix below for details.
TUBE 1 is used for Biochemistry and Flow Cytometry. (Centrifuged in the lab – the supernatant is used for Biochemistry analysis (glucose, protein) and the cellular pellet will be used for flow cytometry if required). If tube 3 is bloodstained, take an aliquot for microbiology.
TUBE 2 is used for Cytology
TUBE 3 is for Microbiological analysis (cell count, Gram stain and culture) and any further infectious testing (e.g. viral PCR, mycobacterial PCR and culture, cryptococcal antigen).
TUBE 4 will be required only for;
(a) Creutzfeld-Jacob Disease (CJD) testing OR
(b) Xanthochromia analysis (wrap the tube in foil to prevent sample deterioration due to light).
TESTS REQUIRED
|
MINIMUM REQUIRED VOLUME (mL)
|
TUBE 1
|
TUBE 2
|
TUBE 3
|
TUBE 4
|
CHEMISTRY
|
1.0
|
|
|
|
ADD FLOW CYTOMETRY
|
Minimum 5.0-7.0
|
|
|
|
CYTOLOGY
|
|
1.0-3.0
|
|
|
MICROBIOLOGY
|
|
|
1.0-2.0
|
|
ADD OTHER INFECTIOUS MARKERS
|
|
|
add 2.0- 5.0-
|
|
ADD MYCOBACTERIAL CULTURE
|
|
|
Add 6.0-10.00
|
|
ADD CJD
|
|
|
|
1.0
|
ADD XANTHOCHROMIA
|
|
|
|
1.0 (foil)
|
Note, the ability to add further tests to a previously collected sample is dependent upon the volume provided. Also, many tests (flow cytometry, cytology) can only be performed on fresh specimens.
Other specialised testing (e.g. oligoclonal bands) will be performed using the supplied samples.
Xanthochromia and CJD analysis cannot be performed on a blood-stained sample.
|
27th April 2021 | Notes | CSF SPECIMEN INFORMATION – LABORATORY PROCESSING
Depending on the tests requested, 3-4 separate tubes will need to be provided. Note that the tubes are numbered so that order of collection can be identified – this order should be carefully followed, so that the optimal sample can be used for the requested tests. It is also important to consider the tests that are required, as this will determine how much CSF is needed in each tube. See the matrix below for details.
TUBE 1 is used for Cytology.
TUBE 2 is used for Glucose, Protein and Flow Cytometry.
(centrifuged in the lab – the supernatant is used for Biochemistry analysis (glucose, protein) and the cellular pellet will be used for flow cytometry if required).
TUBE 3 is for Microbiological analysis (cell count, Gram stain and culture) and any further infectious testing (e.g. viral PCR, mycobacterial PCR and culture, cryptococcal antigen).
TUBE 4 will be required only for;
(a) Creutzfeld-Jacob Disease (CJD) testing OR
(b) Xanthochromia analysis (wrap the tube in foil to prevent sample deterioration due to light).
TESTS REQUIRED
|
MINIMUM REQUIRED VOLUME (mL)
|
TUBE 1
|
TUBE 2
|
TUBE 3
|
TUBE 4
|
CYTOLOGY. CHEMISTRY, MICRO/CULTURE
|
1.0-2.0
|
1.0
|
1.0-2.0
|
Nil
|
ADD FLOW CYTOMETRY
|
|
add 5.0-7.0
|
|
|
ADD OTHER INFECTIOUS MARKERS
|
|
|
add 2.0- 5.0-
|
|
ADD MYCOBACTERIAL CULTURE
|
|
|
Add 10.00
|
|
ADD CJD
|
|
|
|
1.0
|
ADD XANTHOCHROMIA
|
|
|
|
1.0 (foil)
|
Note, the ability to add further tests to a previously collected sample is dependent upon the volume provided. Also, many tests (flow cytometry, cytology) can only be performed on fresh specimens.
Other specialised testing (e.g. oligoclonal bands) will be performed using the supplied samples.
Xanthochromia and CJD analysis cannot be performed on a blood-stained sample. | Depending on the tests requested, 3-4 separate tubes will need to be provided. Note that the tubes are numbered so that order of collection can be identified – this order should be carefully followed, so that the optimal sample can be used for the requested tests. It is also important to consider the tests that are required, as this will determine how much CSF is needed in each tube. See the matrix below for details.
TUBE 1 is used for Biochemistry and Flow Cytometry. (Centrifuged in the lab – the supernatant is used for Biochemistry analysis (glucose, protein) and the cellular pellet will be used for flow cytometry if required). If tube 3 is bloodstained, take an aliquot for microbiology.
TUBE 2 is used for Cytology
TUBE 3 is for Microbiological analysis (cell count, Gram stain and culture) and any further infectious testing (e.g. viral PCR, mycobacterial PCR and culture, cryptococcal antigen).
TUBE 4 will be required only for;
(a) Creutzfeld-Jacob Disease (CJD) testing OR
(b) Xanthochromia analysis (wrap the tube in foil to prevent sample deterioration due to light).
TESTS REQUIRED
|
MINIMUM REQUIRED VOLUME (mL)
|
TUBE 1
|
TUBE 2
|
TUBE 3
|
TUBE 4
|
CHEMISTRY
|
1.0
|
|
|
|
ADD FLOW CYTOMETRY
|
Minimum 5.0-7.0
|
|
|
|
CYTOLOGY
|
|
1.0-3.0
|
|
|
MICROBIOLOGY
|
|
|
1.0-2.0
|
|
ADD OTHER INFECTIOUS MARKERS
|
|
|
add 2.0- 5.0-
|
|
ADD MYCOBACTERIAL CULTURE
|
|
|
Add 6.0-10.00
|
|
ADD CJD
|
|
|
|
1.0
|
ADD XANTHOCHROMIA
|
|
|
|
1.0 (foil)
|
Note, the ability to add further tests to a previously collected sample is dependent upon the volume provided. Also, many tests (flow cytometry, cytology) can only be performed on fresh specimens.
Other specialised testing (e.g. oligoclonal bands) will be performed using the supplied samples.
Xanthochromia and CJD analysis cannot be performed on a blood-stained sample.
|
Recent changes for CMV Serology
Recent changes for CMV Serology |
Date | Field | Changed From | Changed To |
18th January 2023 | Notes | IgG and IgM, IgG Avidity Index performed
| IgG and IgM, IgG Avidity Index performed if IgM is positive
|
18th January 2023 | Laboratory | Microbiology | Biochemistry |
Recent changes for Collagen Binding Assay
Recent changes for Collagen Binding Assay |
Date | Field | Changed From | Changed To |
11th June 2021 | Laboratory | Referred Test | Coagulation |
| Request Group | | CBA |
| Collection & Request Instructions |
Fill to label marker and mix by inversion.
Do not use 1 mL citrate tubes. | Fill to label marker and mix by inversion.
Do not use 1 mL citrate tubes. |
| Processing Instructions |
Specimens kept at room temperature, should be centrifuged ASAP and tested within 4 hours from time of specimen collection. If testing is not completed within 4 hours, plasma should be removed from cells and aliquoted (1mL) into Eppendorf tubes, 2 X 1 mL vials per CBA assay.
Plasma may be frozen @ -20 C for up to 2 weeks or -70 C for up to 6 months. Frost free freezers should not be used. | Specimens kept at room temperature, should be centrifuged ASAP and tested within 4 hours from time of specimen collection. If testing is not completed within 4 hours, plasma should be removed from cells and aliquoted (1mL) into Eppendorf tubes, 2 X 1 mL vials per CBA assay.
Plasma may be frozen @ -20 C for up to 2 weeks or -70 C for up to 6 months. Frost free freezers should not be used. |
| External Laboratory | Austin Pathology | |
| Frequency | | Fortnightly |
Recent changes for Copper-Liver
Recent changes for Copper-Liver |
Date | Field | Changed From | Changed To |
10th May 2021 | External Laboratory | Australian Clinical Labs (formerly Healthscope) | Pacific Laboratory Medicine Services |
Recent changes for EBV Serology
Recent changes for EBV Serology |
Date | Field | Changed From | Changed To |
18th January 2023 | Laboratory | Microbiology | Biochemistry |
Recent changes for Faecal Calprotectin
Recent changes for Faecal Calprotectin |
Date | Field | Changed From | Changed To |
24th August 2022 | Volume (Adults) | | 20 g |
| Minimum/Paediatric Volume | | 1 g |
| Collection & Request Instructions | Samples can be sent frozen. | |
| External Laboratory | | Monash Pathology |
| Frequency | | Weekly |
| Notes |
Contact
Monash University, Eastern Health
Department of Gastroenterology
IBD Gastroenterology Laboratory
Level 1, 5 Arnold St, Box Hill Vic 3128
Ph: 03 9895 4632
Email: ehcs.ibd@monash.edu
Link to test information and costs :
http://www.med.monash.edu.au/ehcs/services/metabolite/calprotectindrsinformation.html |
|
24th August 2022 | Request Group | | CALPRO |
Recent changes for Flavivirus Serology
Recent changes for Flavivirus Serology |
Date | Field | Changed From | Changed To |
1st February 2023 | Alternative names | Dengue fever serology, Kunjin serology, Murray Valley Encephalitis serology | Dengue fever serology, Kunjin serology, Murray Valley Encephalitis serology, Japanese B encephalitis |
| Request Group | SDENG, OPCR, (SMVE, SJBE) | SDENG, SMVE, SJBE |
31st January 2023 | Preferred Specimen Type | Blood | Blood Serum |
| Preferred Container Type | EDTA | EDTA Serum tube with gel |
31st January 2023 | Request Group | OPCR | SDENG, OPCR, (SMVE, SJBE) |
| Notes | Procedure detects flaviviruses including Murray Valley encephalitis, dengue and kunjin viruses | PCR testing detects flaviviruses including Murray Valley encephalitis, dengue and kunjin viruses. Dengue specific serology tests for IgG, IgM, and NS1 antigen |
Recent changes for Fluconazole
Recent changes for Fluconazole |
Date | Field | Changed From | Changed To |
14th October 2021 | Collection & Request Instructions |
|
|
| Processing Instructions |
Spin, separate Plasma and freeze.
Packed by certified personnel.
Results:02-83829100 or 02-82829190 | Spin and separate Plasma.
|
| External Laboratory | Sydpath Pathology, St Vincent's Hospital NSW | Alfred Hospital |
Recent changes for Free PSA
Recent changes for Free PSA |
Date | Field | Changed From | Changed To |
21st June 2021 | External Laboratory | Melbourne Pathology | |
| Method | | Free PSA is now performed in house at Box Hill Hospital.
Chemical Pathologist approval is still required |
Recent changes for Hepatitis B Viral Load
Recent changes for Hepatitis B Viral Load |
Date | Field | Changed From | Changed To |
2nd February 2023 | Processing Instructions | Perfomed by Abbott Alinity M | |
| Method | | Performed by Abbott Alinity M |
2nd February 2023 | Assay name | Hepatitis B Quantitation | Hepatitis B Viral Load |
| Alternative names | Hepatitis B Viral Load | Hepatitis B Viral Load, Hep B |
| Laboratory | Referred Test | Microbiology |
| Request Group | HEPBVL | HBVL |
| Processing Instructions | Contact VIDRL 9342 9615 | Perfomed by Abbott Alinity M |
| External Laboratory | Victorian Infectious Diseases Reference Laboratory (V.I.D.R.L) | |
Recent changes for IgG Subclasses
Recent changes for IgG Subclasses |
Date | Field | Changed From | Changed To |
13th September 2022 | External Laboratory | Austin Pathology | |
Recent changes for Infectious Mononucleosis Screen
Recent changes for Infectious Mononucleosis Screen |
Date | Field | Changed From | Changed To |
30th March 2022 | Preferred Specimen Type | Serum | Serum Blood |
| Optional Specimen Type | | Plasma |
| Preferred Container Type | Serum tube with gel | Serum tube with gel EDTA EDTA - Paediatric |
Recent changes for Infliximab
Recent changes for Infliximab |
Date | Field | Changed From | Changed To |
5th April 2022 | Alternative names | | Inflectra, Remicade |
| Volume (Adults) | | 2 mL |
| Processing Instructions | After centifugation the serum must be aliquoted into the special aliquot tube provided and freeze. | Store at 4 C.
|
| External Laboratory | South Western Area Pathology,Liverpool Hospital | Dorevitch Pathology |
| External Transport Instructions | Samples to be batched and sent to SSWAPs frozen | Send to Testing Laboratory at 4 C |
| Frequency | Monthly | Weekly |
Recent changes for Iron - Liver
Recent changes for Iron - Liver |
Date | Field | Changed From | Changed To |
10th May 2021 | Collection & Request Instructions | Liver biopsy specimen to be placed directly into a clear, dry container and delivered to the laboraory as soon as possible. Minimum sample size for accurate analysis is 1-2 mg. | Liver biopsy specimen to be placed directly into a clear, dry container and delivered to the laboraory as soon as possible. Minimum sample size for accurate analysis is 1-2 mg. |
| External Laboratory | Australian Clinical Labs (formerly Healthscope) | Pacific Laboratory Medicine Services |
| Notes |
Hepatic Iron Index |
Hepatic Iron Concentration
|
Age in years
|
Values > 2 indicate haemochromatosis
Liver biopsy specimen to be placed directly into a clear, dry container and delivered to the laboraory as soon as possible. Minimum sample size for accurate analysis is 1-2 mg. |
Hepatic Iron Index |
Hepatic Iron Concentration
|
Age in years
|
Values > 2 indicate haemochromatosis
Liver biopsy specimen to be placed directly into a clear, dry container and delivered to the laboraory as soon as possible. Minimum sample size for accurate analysis is 1-2 mg. |
Recent changes for Itraconazole
Recent changes for Itraconazole |
Date | Field | Changed From | Changed To |
14th July 2022 | Volume (Adults) | 2 mL | 5 mL |
| Minimum/Paediatric Volume | 1 mL | |
| Preferred Container Type | Lithium heparin no gel | EDTA |
| Processing Instructions | Spin, separate and freeze plasma. | Spin and separate plasma. |
| External Laboratory | Women's & Children's Hospital (Adelaide) | Alfred Hospital |
| External Transport Instructions | Packed by certified personnal - transport frozen.
Results : 08-81617000, 08-81616732. | |
| Frequency | Monthly | Twice weekly |
| Notes | | Dose information should be provided and length of therapy must be >5 days. |
Recent changes for Karyotype/Microarray - TISSUE
Recent changes for Karyotype/Microarray - TISSUE |
Date | Field | Changed From | Changed To |
6th March 2022 | Assay name | Cytogenetics / Karyotype - TISSUE | Karyotype/Microarray - TISSUE |
14th December 2021 | Assay name | CytogeneticsKaryotype - TISSUE | Cytogenetics / Karyotype - TISSUE |
14th December 2021 | Assay name | CYTOGENETICS - TISSUE | CytogeneticsKaryotype - TISSUE |
| Alternative names | KARYOTYPE
CHROMOSOME STUDIES - TISSUE
TISSUE CULTURE
MOLECULAR KARYOTYPE
MICROARRAY | KARYOTYPE
CHROMOSOME STUDIES - TISSUE
TISSUE CULTURE
MOLECULAR KARYOTYPE
MICROARRAY
FIBROBLAST CULTURE |
14th December 2021 | Assay name | CYTOGENETICS - TISSUE NON MALIGNANT (CONSTITUTIONAL) | CYTOGENETICS - TISSUE |
| Frequency | Weekly | Daily |
Recent changes for Lamotrigine
Recent changes for Lamotrigine |
Date | Field | Changed From | Changed To |
14th July 2022 | Alternative names | | Lamictal |
| Volume (Adults) | 2 mL | 3 mL |
| Minimum/Paediatric Volume | 1 mL | 3 mL |
| Processing Instructions | Spin aliquot plasma. (Send either plasma or whole blood) | Spin & aliquot plasma |
| External Laboratory | Australian Clinical Labs (formerly Healthscope) | Alfred Hospital |
Recent changes for Leucocyte Immunophenotyping
Recent changes for Leucocyte Immunophenotyping |
Date | Field | Changed From | Changed To |
15th October 2021 | Notes | Samples must be marked 'URGENT', must reach the laboratory as soon as possible.
All out-of-hours testing requires notification.
Time and date of collection must be recorded on request form.
Whole blood - 1 x EDTA sample, do not spin. Store at room temperature. An FBE must also be requested, and needs a separate EDTA sample.
Bone marrow aspirate - Sample into EDTA tube
Tissue - Fresh (if to be shared with other departments for other tests eg. Histopathology or Microbiology) or RPMI fluid (especially if specimen is taken out of hours). This fluid is obtainable from: BH Lab 9895 3884, MH Lab: 9871 3572 or AH Lab: 9764 6136.
CSF/other fluids
Depending on the tests requested, 3-4 separate tubes will need to be provided. Note that the tubes are numbered so that order of collection can be identified – this order should be carefully followed, so that the optimal sample can be used for the requested tests. It is also important to consider the tests that are required, as this will determine how much CSF is needed in each tube. See the matrix below for details.
TUBE 1 is used for Biochemistry and Flow Cytometry. (Centrifuged in the lab – the supernatant is used for Biochemistry analysis (glucose, protein) and the cellular pellet will be used for flow cytometry if required). If tube 3 is bloodstained, take an aliquot for microbiology.
TUBE 2 is used for Cytology
TUBE 3 is for Microbiological analysis (cell count, Gram stain and culture) and any further infectious testing (e.g. viral PCR, mycobacterial PCR and culture, cryptococcal antigen).
TUBE 4 will be required only for;
(a) Creutzfeld-Jacob Disease (CJD) testing OR
(b) Xanthochromia analysis (wrap the tube in foil to prevent sample deterioration due to light).
TESTS REQUIRED
|
MINIMUM REQUIRED VOLUME (mL)
|
TUBE 1
|
TUBE 2
|
TUBE 3
|
TUBE 4
|
CHEMISTRY
|
1.0
|
|
|
|
ADD FLOW CYTOMETRY
|
Minimum 5.0-7.0
|
|
|
|
CYTOLOGY
|
|
1.0-3.0
|
|
|
MICROBIOLOGY
|
|
|
1.0-2.0
|
|
ADD OTHER INFECTIOUS MARKERS
|
|
|
add 2.0- 5.0-
|
|
ADD MYCOBACTERIAL CULTURE
|
|
|
Add 6.0-10.00
|
|
ADD CJD
|
|
|
|
1.0
|
ADD XANTHOCHROMIA
|
|
|
|
1.0 (foil)
|
Note, the ability to add further tests to a previously collected sample is dependent upon the volume provided. Also, many tests (flow cytometry, cytology) can only be performed on fresh specimens.
Other specialised testing (e.g. oligoclonal bands) will be performed using the supplied samples.
Xanthochromia and CJD analysis cannot be performed on a blood-stained sample | Samples must be marked 'URGENT', must reach the laboratory as soon as possible.
All out-of-hours testing requires notification.
Time and date of collection must be recorded on request form.
Whole blood - 1 x EDTA sample, do not spin. Store at room temperature. An FBE and BLOOD FILM must also be requested, and needs a separate EDTA sample.
Bone marrow aspirate - Sample into EDTA tube
Tissue - Fresh (if to be shared with other departments for other tests eg. Histopathology or Microbiology) or RPMI fluid (especially if specimen is taken out of hours). This fluid is obtainable from: BH Lab 9895 3884, MH Lab: 9871 3572 or AH Lab: 9764 6136.
CSF/other fluids
Depending on the tests requested, 3-4 separate tubes will need to be provided. Note that the tubes are numbered so that order of collection can be identified – this order should be carefully followed, so that the optimal sample can be used for the requested tests. It is also important to consider the tests that are required, as this will determine how much CSF is needed in each tube. See the matrix below for details.
TUBE 1 is used for Biochemistry and Flow Cytometry. (Centrifuged in the lab – the supernatant is used for Biochemistry analysis (glucose, protein) and the cellular pellet will be used for flow cytometry if required). If tube 3 is bloodstained, take an aliquot for microbiology.
TUBE 2 is used for Cytology
TUBE 3 is for Microbiological analysis (cell count, Gram stain and culture) and any further infectious testing (e.g. viral PCR, mycobacterial PCR and culture, cryptococcal antigen).
TUBE 4 will be required only for;
(a) Creutzfeld-Jacob Disease (CJD) testing OR
(b) Xanthochromia analysis (wrap the tube in foil to prevent sample deterioration due to light).
TESTS REQUIRED
|
MINIMUM REQUIRED VOLUME (mL)
|
TUBE 1
|
TUBE 2
|
TUBE 3
|
TUBE 4
|
CHEMISTRY
|
1.0
|
|
|
|
ADD FLOW CYTOMETRY
|
Minimum 5.0-7.0
|
|
|
|
CYTOLOGY
|
|
1.0-3.0
|
|
|
MICROBIOLOGY
|
|
|
1.0-2.0
|
|
ADD OTHER INFECTIOUS MARKERS
|
|
|
add 2.0- 5.0-
|
|
ADD MYCOBACTERIAL CULTURE
|
|
|
Add 6.0-10.00
|
|
ADD CJD
|
|
|
|
1.0
|
ADD XANTHOCHROMIA
|
|
|
|
1.0 (foil)
|
Note, the ability to add further tests to a previously collected sample is dependent upon the volume provided. Also, many tests (flow cytometry, cytology) can only be performed on fresh specimens.
Other specialised testing (e.g. oligoclonal bands) will be performed using the supplied samples.
Xanthochromia and CJD analysis cannot be performed on a blood-stained sample |
14th October 2021 | Notes |
Samples must be marked 'URGENT', must reach the laboratory as soon as possible.
All out-of-hours testing requires notification.
Time and date of collection must be recorded on request form.
Whole blood - 1 x EDTA sample, do not spin. Store at room temperature. An FBE must also be requested, and needs a separate EDTA sample.
Bone marrow aspirate - Sample into EDTA tube
Tissue - Fresh (if to be shared with other departments for other tests eg. Histopathology or Microbiology) or RPMI fluid (especially if specimen is taken out of hours). This fluid is obtainable from: BH Lab 9895 3884, MH Lab: 9871 3572 or AH Lab: 9764 6136.
CSF/other fluids - at least 2mL is preferred
Depending on the tests requested, 3-4 separate tubes will need to be provided. Note that the tubes are numbered so that order of collection can be identified – this order should be carefully followed, so that the optimal sample can be used for the requested tests. It is also important to consider the tests that are required, as this will determine how much CSF is needed in each tube. See the matrix below for details.
TUBE 1 is used for Biochemistry and Flow Cytometry. (Centrifuged in the lab – the supernatant is used for Biochemistry analysis (glucose, protein) and the cellular pellet will be used for flow cytometry if required). If tube 3 is bloodstained, take an aliquot for microbiology.
TUBE 2 is used for Cytology
TUBE 3 is for Microbiological analysis (cell count, Gram stain and culture) and any further infectious testing (e.g. viral PCR, mycobacterial PCR and culture, cryptococcal antigen).
TUBE 4 will be required only for;
(a) Creutzfeld-Jacob Disease (CJD) testing OR
(b) Xanthochromia analysis (wrap the tube in foil to prevent sample deterioration due to light).
TESTS REQUIRED
|
MINIMUM REQUIRED VOLUME (mL)
|
TUBE 1
|
TUBE 2
|
TUBE 3
|
TUBE 4
|
CHEMISTRY
|
1.0
|
|
|
|
ADD FLOW CYTOMETRY
|
Minimum 5.0-7.0
|
|
|
|
CYTOLOGY
|
|
1.0-3.0
|
|
|
MICROBIOLOGY
|
|
|
1.0-2.0
|
|
ADD OTHER INFECTIOUS MARKERS
|
|
|
add 2.0- 5.0-
|
|
ADD MYCOBACTERIAL CULTURE
|
|
|
Add 6.0-10.00
|
|
ADD CJD
|
|
|
|
1.0
|
ADD XANTHOCHROMIA
|
|
|
|
1.0 (foil)
|
Note, the ability to add further tests to a previously collected sample is dependent upon the volume provided. Also, many tests (flow cytometry, cytology) can only be performed on fresh specimens.
Other specialised testing (e.g. oligoclonal bands) will be performed using the supplied samples.
Xanthochromia and CJD analysis cannot be performed on a blood-stained sample |
Samples must be marked 'URGENT', must reach the laboratory as soon as possible.
All out-of-hours testing requires notification.
Time and date of collection must be recorded on request form.
Whole blood - 1 x EDTA sample, do not spin. Store at room temperature. An FBE must also be requested, and needs a separate EDTA sample.
Bone marrow aspirate - Sample into EDTA tube
Tissue - Fresh (if to be shared with other departments for other tests eg. Histopathology or Microbiology) or RPMI fluid (especially if specimen is taken out of hours). This fluid is obtainable from: BH Lab 9895 3884, MH Lab: 9871 3572 or AH Lab: 9764 6136.
CSF/other fluids
Depending on the tests requested, 3-4 separate tubes will need to be provided. Note that the tubes are numbered so that order of collection can be identified – this order should be carefully followed, so that the optimal sample can be used for the requested tests. It is also important to consider the tests that are required, as this will determine how much CSF is needed in each tube. See the matrix below for details.
TUBE 1 is used for Biochemistry and Flow Cytometry. (Centrifuged in the lab – the supernatant is used for Biochemistry analysis (glucose, protein) and the cellular pellet will be used for flow cytometry if required). If tube 3 is bloodstained, take an aliquot for microbiology.
TUBE 2 is used for Cytology
TUBE 3 is for Microbiological analysis (cell count, Gram stain and culture) and any further infectious testing (e.g. viral PCR, mycobacterial PCR and culture, cryptococcal antigen).
TUBE 4 will be required only for;
(a) Creutzfeld-Jacob Disease (CJD) testing OR
(b) Xanthochromia analysis (wrap the tube in foil to prevent sample deterioration due to light).
TESTS REQUIRED
|
MINIMUM REQUIRED VOLUME (mL)
|
TUBE 1
|
TUBE 2
|
TUBE 3
|
TUBE 4
|
CHEMISTRY
|
1.0
|
|
|
|
ADD FLOW CYTOMETRY
|
Minimum 5.0-7.0
|
|
|
|
CYTOLOGY
|
|
1.0-3.0
|
|
|
MICROBIOLOGY
|
|
|
1.0-2.0
|
|
ADD OTHER INFECTIOUS MARKERS
|
|
|
add 2.0- 5.0-
|
|
ADD MYCOBACTERIAL CULTURE
|
|
|
Add 6.0-10.00
|
|
ADD CJD
|
|
|
|
1.0
|
ADD XANTHOCHROMIA
|
|
|
|
1.0 (foil)
|
Note, the ability to add further tests to a previously collected sample is dependent upon the volume provided. Also, many tests (flow cytometry, cytology) can only be performed on fresh specimens.
Other specialised testing (e.g. oligoclonal bands) will be performed using the supplied samples.
Xanthochromia and CJD analysis cannot be performed on a blood-stained sample |
14th October 2021 | Alternative names | CD4/CD8 Counts, Cell Surface Markers, FACS Analysis, Flow Cytometry | Cell Surface Markers, FACS Analysis, Flow Cytometry, Immunophenotyping |
| Request Group | FLOW | LYMP, LEUK |
| Collection & Request Instructions | Sample store at Room temperature. Samples are not stable for more than 24 hours. Hence they must be received by the Flow Cytometry lab at Box Hill within normal business hours Monday to Friday.
Cutoff for outpatient sample collection on Fridays is at 11.30 AM and MUST reach the laboratory before 4.00PM.
Flow cytometric analysis of cells stained by immunofluorescence with a range of antibodies against lymphoid and myeloid markers.
The panel of markers used will be appropriate to the clinical comments supplied , or as arranged by prior consultation. | Sample store at Room temperature. Samples are not stable for more than 24 hours. Hence they must be received by the Flow Cytometry lab at Box Hill within normal business hours Monday to Friday.
Cut-off for outpatient sample collection on Fridays is at 11.30 AM and MUST reach the laboratory before 4.00PM.
Flow cytometric analysis of cells stained by immunofluorescence with a range of antibodies against lymphoid and myeloid markers.
The panel of markers used will be appropriate to the panel selected and the clinical comments supplied , or as arranged by prior consultation |
| Notes |
Samples must be marked 'URGENT', must reach the laboratory as soon as possible.
All out-of-hours testing requires notification.
Time and date of collection must be recorded on request form.
Whole blood - 1 x EDTA sample, do not spin. Store at room temperature. An FBE must also be requested, needs a separate EDTA sample.
Bone marrow aspirate - Sample into EDTA tube
Tissue - Fresh (if to be shared with other departments for other tests eg. Histopathology or Microbiology) or RPMI fluid (especially if specimen is taken out of hours). This fluid is obtainable from: BH Lab 9895 3884, MH Lab: 9871 3572 or AH Lab: 9764 6136.
CSF/other fluids - at least 2mL is preferred
Collect CSF into three separate, sterile, screw-capped tubes provided (labelled 1,2 & 3)
Tube 1 is for Biochemistry (for Protein and Glucose) 0.5ml (10 drops) required
Tube 2 for Cytology 1.0ml (20 drops) and/or Flow Cytometry (6ml)
For Flow Cytometry a minimum of 6ml is required in Tube 2
For Flow Cytometry and Cytology a minimum of 7ml is required in Tube 2
Tube 3 for Microbiology upto 10ml (Full tube)
If querying CJD the laboratory requires a 4th unopened tube containing at least 1ml of non-bloodstained CSF.
Xanthochromia investigation requires a 4th tube containing 1ml of CSF . |
Samples must be marked 'URGENT', must reach the laboratory as soon as possible.
All out-of-hours testing requires notification.
Time and date of collection must be recorded on request form.
Whole blood - 1 x EDTA sample, do not spin. Store at room temperature. An FBE must also be requested, and needs a separate EDTA sample.
Bone marrow aspirate - Sample into EDTA tube
Tissue - Fresh (if to be shared with other departments for other tests eg. Histopathology or Microbiology) or RPMI fluid (especially if specimen is taken out of hours). This fluid is obtainable from: BH Lab 9895 3884, MH Lab: 9871 3572 or AH Lab: 9764 6136.
CSF/other fluids - at least 2mL is preferred
Depending on the tests requested, 3-4 separate tubes will need to be provided. Note that the tubes are numbered so that order of collection can be identified – this order should be carefully followed, so that the optimal sample can be used for the requested tests. It is also important to consider the tests that are required, as this will determine how much CSF is needed in each tube. See the matrix below for details.
TUBE 1 is used for Biochemistry and Flow Cytometry. (Centrifuged in the lab – the supernatant is used for Biochemistry analysis (glucose, protein) and the cellular pellet will be used for flow cytometry if required). If tube 3 is bloodstained, take an aliquot for microbiology.
TUBE 2 is used for Cytology
TUBE 3 is for Microbiological analysis (cell count, Gram stain and culture) and any further infectious testing (e.g. viral PCR, mycobacterial PCR and culture, cryptococcal antigen).
TUBE 4 will be required only for;
(a) Creutzfeld-Jacob Disease (CJD) testing OR
(b) Xanthochromia analysis (wrap the tube in foil to prevent sample deterioration due to light).
TESTS REQUIRED
|
MINIMUM REQUIRED VOLUME (mL)
|
TUBE 1
|
TUBE 2
|
TUBE 3
|
TUBE 4
|
CHEMISTRY
|
1.0
|
|
|
|
ADD FLOW CYTOMETRY
|
Minimum 5.0-7.0
|
|
|
|
CYTOLOGY
|
|
1.0-3.0
|
|
|
MICROBIOLOGY
|
|
|
1.0-2.0
|
|
ADD OTHER INFECTIOUS MARKERS
|
|
|
add 2.0- 5.0-
|
|
ADD MYCOBACTERIAL CULTURE
|
|
|
Add 6.0-10.00
|
|
ADD CJD
|
|
|
|
1.0
|
ADD XANTHOCHROMIA
|
|
|
|
1.0 (foil)
|
Note, the ability to add further tests to a previously collected sample is dependent upon the volume provided. Also, many tests (flow cytometry, cytology) can only be performed on fresh specimens.
Other specialised testing (e.g. oligoclonal bands) will be performed using the supplied samples.
Xanthochromia and CJD analysis cannot be performed on a blood-stained sample |
20th July 2021 | Assay name | Leucocyte Immunophenotyping | T Cell Subsets |
| Alternative names | CD4/CD8 Counts, Cell Surface Markers, FACS Analysis, Flow Cytometry | CD2, CD3, CD4, CD8 |
| Preferred Container Type | EDTA Sterile Jar | EDTA Lithium heparin no gel |
| Collection & Request Instructions | Sample store at Room temperature. Samples are not stable for more than 24 hours. Hence they must be received by the Flow Cytometry lab at Box Hill within normal business hours Monday to Friday.
Cutoff for outpatient sample collection on Fridays is at 11.30 AM and MUST reach the laboratory before 4.00PM.
Flow cytometric analysis of cells stained by immunofluorescence with a range of antibodies against lymphoid and myeloid markers.
The panel of markers used will be appropriate to the clinical comments supplied , or as arranged by prior consultation. | The samples are not stable for more than 24 hours. Hence all samples must be recieved in the laboratory at Box Hill Monday to Friday within business hours.
Cutoff for outpatient sample collection on Fridays is at 11.30 AM and MUST reach the laboratory before 4.00PM. |
| Processing Instructions | | Prefer EDTA sample.
Do not spin, store at room temperature. |
| Notes |
Samples must be marked 'URGENT', must reach the laboratory as soon as possible.
All out-of-hours testing requires notification.
Time and date of collection must be recorded on request form.
Whole blood - 1 x EDTA sample, do not spin. Store at room temperature. An FBE must also be requested, needs a separate EDTA sample.
Bone marrow aspirate - Sample into EDTA tube
Tissue - Fresh (if to be shared with other departments for other tests eg. Histopathology or Microbiology) or RPMI fluid (especially if specimen is taken out of hours). This fluid is obtainable from: BH Lab 9895 3884, MH Lab: 9871 3572 or AH Lab: 9764 6136.
CSF/other fluids - at least 2mL is preferred
Collect CSF into three separate, sterile, screw-capped tubes provided (labelled 1,2 & 3)
Tube 1 is for Biochemistry (for Protein and Glucose) 0.5ml (10 drops) required
Tube 2 for Cytology 1.0ml (20 drops) and/or Flow Cytometry (6ml)
For Flow Cytometry a minimum of 6ml is required in Tube 2
For Flow Cytometry and Cytology a minimum of 7ml is required in Tube 2
Tube 3 for Microbiology upto 10ml (Full tube)
If querying CJD the laboratory requires a 4th unopened tube containing at least 1ml of non-bloodstained CSF.
Xanthochromia investigation requires a 4th tube containing 1ml of CSF . | Used to monitor T-cell numbers
Preferred EDTA sample. Do not spin, store at room temperature. |
19th July 2021 | Collection & Request Instructions | Sample store at Room temperature. Samples are not stable for more than 24 hours. Hence they must be recieved by the Flow Cytometry lab at Box Hill within normal business hours Monday to Friday.
Cutoff for outpatirnt sample collection on Fridays is at 12:00 PM and MUST reach the laboratory by 4.00PM.
Flow cytometric analysis of cells stained by immunofluorescence with a range of antibodies against lymphoid and myeloid markers.
The panel of markers used will be appropriate to the clinical comments supplied , or as arranged by prior consultation. | Sample store at Room temperature. Samples are not stable for more than 24 hours. Hence they must be received by the Flow Cytometry lab at Box Hill within normal business hours Monday to Friday.
Cutoff for outpatient sample collection on Fridays is at 11.30 AM and MUST reach the laboratory before 4.00PM.
Flow cytometric analysis of cells stained by immunofluorescence with a range of antibodies against lymphoid and myeloid markers.
The panel of markers used will be appropriate to the clinical comments supplied , or as arranged by prior consultation. |
19th July 2021 | Collection & Request Instructions |
Sample store at Room temperature. Samples are not stable for more than 24 hours. Hence they must be recieved by the Flow Cytometry lab at Box Hill within normal business hours Monday to Friday
Flow cytometric analysis of cells stained by immunofluorescence with a range of antibodies against lymphoid and myeloid markers.
The panel of markers used will be appropriate to the clinical comments supplied , or as arranged by prior consultation. | Sample store at Room temperature. Samples are not stable for more than 24 hours. Hence they must be recieved by the Flow Cytometry lab at Box Hill within normal business hours Monday to Friday.
Cutoff for outpatirnt sample collection on Fridays is at 12:00 PM and MUST reach the laboratory by 4.00PM.
Flow cytometric analysis of cells stained by immunofluorescence with a range of antibodies against lymphoid and myeloid markers.
The panel of markers used will be appropriate to the clinical comments supplied , or as arranged by prior consultation. |
| Notes |
-
Samples must be marked 'URGENT', must reach the laboratory as soon as possible.
-
All out-of-hours testing requires notification.
-
Time and date of collection must be recorded on request form.
Whole blood - 1 x EDTA sample, do not spin. Store at room temperature. An FBE must also be requested, needs a separate EDTA sample.
Bone marrow aspirate - Sample into EDTA tube
Tissue - Fresh (if to be shared with other departments for other tests eg. Histopathology or Microbiology) or RPMI fluid (especially if specimen is taken out of hours). This fluid is obtainable from: BH Lab 9895 3884, MH Lab: 9871 3572 or AH Lab: 9764 6136.
-
CSF/other fluids - at least 2mL is preferred
-
Collect CSF into three separate, sterile, screw-capped tubes provided (labelled 1,2 & 3)
-
Tube 1 is for Biochemistry (for Protein and Glucose) 0.5ml (10 drops) required
-
Tube 2 for Cytology 1.0ml (20 drops) and/or Flow Cytometry (6ml)
-
For Flow Cytometry a minimum of 6ml is required in Tube 2
-
For Flow Cytometry and Cytology a minimum of 7ml is required in Tube 2
-
Tube 3 for Microbiology upto 10ml (Full tube)
If querying CJD the laboratory requires a 4th unopened tube containing at least 1ml of non-bloodstained CSF.
Xanthochromia investigation requires a 4th tube containing 1ml of CSF .
|
Samples must be marked 'URGENT', must reach the laboratory as soon as possible.
All out-of-hours testing requires notification.
Time and date of collection must be recorded on request form.
Whole blood - 1 x EDTA sample, do not spin. Store at room temperature. An FBE must also be requested, needs a separate EDTA sample.
Bone marrow aspirate - Sample into EDTA tube
Tissue - Fresh (if to be shared with other departments for other tests eg. Histopathology or Microbiology) or RPMI fluid (especially if specimen is taken out of hours). This fluid is obtainable from: BH Lab 9895 3884, MH Lab: 9871 3572 or AH Lab: 9764 6136.
CSF/other fluids - at least 2mL is preferred
Collect CSF into three separate, sterile, screw-capped tubes provided (labelled 1,2 & 3)
Tube 1 is for Biochemistry (for Protein and Glucose) 0.5ml (10 drops) required
Tube 2 for Cytology 1.0ml (20 drops) and/or Flow Cytometry (6ml)
For Flow Cytometry a minimum of 6ml is required in Tube 2
For Flow Cytometry and Cytology a minimum of 7ml is required in Tube 2
Tube 3 for Microbiology upto 10ml (Full tube)
If querying CJD the laboratory requires a 4th unopened tube containing at least 1ml of non-bloodstained CSF.
Xanthochromia investigation requires a 4th tube containing 1ml of CSF . |
Recent changes for Linezolid
Recent changes for Linezolid |
Date | Field | Changed From | Changed To |
14th July 2022 | Volume (Adults) | 2 mL | 3 mL |
| Minimum/Paediatric Volume | | 3 mL |
| Optional Container Type | | Lithium heparin no gel |
| Collection & Request Instructions | Deliver to laboratory ASAP. | A separate unopened specimen is required for this test. |
| Processing Instructions | Spin, separate and freeze plasma. | |
| External Laboratory | Sydpath Pathology, St Vincent's Hospital NSW | Alfred Hospital |
| External Transport Instructions | | Send at 4 C. |
| Frequency | As required | Weekly |
| Method | Results: Clinical Pharmacology on 02 8382 9184. | |
Recent changes for Lupus Anticoagulant Screen
Recent changes for Lupus Anticoagulant Screen |
Date | Field | Changed From | Changed To |
3rd May 2021 | External Transport Instructions | Samples can be transported frozen to the laboratory if the facilities are available to double spin and aliquot these sample. This has to be done within 4 hours.
Unspun sample is stable for up to 4 hours and must be transport to laboratory immediately. (Sample must be double spun, aliquoted and frozen within 4 hours from time of collection). | Samples can be transported frozen to the laboratory if the facilities are available to double spin and aliquot these samples. This has to be performed within 4 hours of collection.
Unspun sample is stable for up to 4 hours and must be transport to laboratory immediately. (Sample must be double spun, aliquoted and frozen within 4 hours from time of collection). |
3rd May 2021 | Collection & Request Instructions | Notification of anticoagulant therapy is essential
One (1x) Sodium citrate tubes is required. Do not use 1 ml citrate tubes.
Filled to label marker and mixed by inversion
The patient must not be receiving heparin
Transport to laboratory immediately. (Sample must be double spun and frozen within 4 hours from time of collection).
Tests performed:
INR, APTT, APTT Mix, Silica Clotting Time and Dilute Russell's Viper venom time (LA screen/Confirm).
| Notification of anticoagulant therapy is essential
One (1x) Sodium citrate tubes is required. Do not use 1 ml citrate tubes.
Filled to label marker and mixed by inversion
The patient must not be receiving heparin
Unspun sample is stable for up to 4 hours and must be transport to laboratory immediately. (Sample must be double spun, aliquoted and frozen within 4 hours from time of collection).
Tests performed:
INR, APTT, APTT Mix, Silica Clotting Time and Dilute Russell's Viper venom time (LA screen/Confirm).
|
| Processing Instructions | Transport sample to laboratory within 4 hours of collection.
The sample MUST be double spun and frozen within the 4 hour time limit. | Unspun sample is stable for up to 4 hours and must be transport to laboratory immediately. (Sample must be double spun, aliquoted and frozen within 4 hours from time of collection). |
| External Transport Instructions | | Samples can be transported frozen to the laboratory if the facilities are available to double spin and aliquot these sample. This has to be done within 4 hours.
Unspun sample is stable for up to 4 hours and must be transport to laboratory immediately. (Sample must be double spun, aliquoted and frozen within 4 hours from time of collection). |
3rd May 2021 | Collection & Request Instructions | Notification of anticoagulant therapy is essential
One (1x) Sodium citrate tubes is required. Do not use 1 ml citrate tubes.
Filled to label marker and mixed by inversion
The patient must not be receiving heparin
Transport to laboratory immediately. (maximum of 4 hours from time of collection).
Tests performed:
INR, APTT, APTT Mix, Silica Clotting Time and Dilute Russell's Viper venom time (LA screen/Confirm).
| Notification of anticoagulant therapy is essential
One (1x) Sodium citrate tubes is required. Do not use 1 ml citrate tubes.
Filled to label marker and mixed by inversion
The patient must not be receiving heparin
Transport to laboratory immediately. (Sample must be double spun and frozen within 4 hours from time of collection).
Tests performed:
INR, APTT, APTT Mix, Silica Clotting Time and Dilute Russell's Viper venom time (LA screen/Confirm).
|
| Processing Instructions | | Transport sample to laboratory within 4 hours of collection.
The sample MUST be double spun and frozen within the 4 hour time limit. |
Recent changes for Lymphocyte subsets
Recent changes for Lymphocyte subsets |
Date | Field | Changed From | Changed To |
14th October 2021 | Alternative names | | CD3 count, CD4 count, CD8 count, CD4:CD8 ratio
CD19 count, CD56 count, flow cytometry |
14th October 2021 | Alternative names | CD19 count & CD56 count, CD4 count, CD4 count, CD4:CD8 ratio
Lymphocyte subsets include: CD3 count, CD4:CD8 ratio, CD8 count, CD8 count, T subsets include: CD3 count | T subsets include: CD3 count, CD4 count, CD8 count, CD4:CD8 ratio
Lymphocyte subsets include: CD3 count, CD4 count, CD8 count, CD4:CD8 ratio, CD19 count & CD56 count
Flow Cytometry |
| Collection & Request Instructions | Sample store at Room temperature. Samples are not stable for more than 24 hours. Hence they must be received by the Flow Cytometry lab at Box Hill within normal business hours Monday to Friday.
Cut-off for outpatient sample collection on Fridays is at 11.30 AM and MUST reach the laboratory before 4.00PM.
Flow cytometric analysis of cells stained by immunofluorescence with a range of antibodies against lymphoid and myeloid markers.
The panel of markers used will be appropriate to the panel selected and the clinical comments supplied, or as arranged by prior consultation. | Sample store at ROOM TEMPERATURE. Samples are not stable for more than 24 hours. Hence they must be received by the Flow Cytometry lab at Box Hill within normal business hours Monday to Friday.
Cut-off for outpatient sample collection on Fridays is at 11.30 AM and MUST reach the laboratory before 4.00PM.
Flow cytometric analysis of cells stained by immunofluorescence with a range of antibodies against lymphoid and myeloid markers.
The panel of markers used will be appropriate to the panel selected and the clinical comments supplied, or as arranged by prior consultation. |
14th October 2021 | Collection & Request Instructions | The samples are not stable for more than 24 hours. Hence all samples must be recieved in the laboratory at Box Hill Monday to Friday within business hours.
Cutoff for outpatient sample collection on Fridays is at 11.30 AM and MUST reach the laboratory before 4.00PM.
| Sample store at Room temperature. Samples are not stable for more than 24 hours. Hence they must be received by the Flow Cytometry lab at Box Hill within normal business hours Monday to Friday.
Cut-off for outpatient sample collection on Fridays is at 11.30 AM and MUST reach the laboratory before 4.00PM.
Flow cytometric analysis of cells stained by immunofluorescence with a range of antibodies against lymphoid and myeloid markers.
The panel of markers used will be appropriate to the panel selected and the clinical comments supplied, or as arranged by prior consultation. |
| Frequency | | Daily |
| Notes | Used to monitor T-cell numbers
Preferred EDTA sample. Do not spin, store at room temperature. | Samples must be marked 'URGENT', must reach the laboratory as soon as possible.
All out-of-hours testing requires notification.
Time and date of collection must be recorded on request form.
Whole blood - 1 x EDTA sample, do not spin. Store at room temperature. An FBE must also be requested, and needs a separate EDTA sample. |
14th October 2021 | Assay name | T Cell Subsets | Lymphocyte subsets |
| Alternative names | CD2, CD3, CD4, CD8 | T subsets include: CD3 count, CD4 count, CD8 count, CD4:CD8 ratio
Lymphocyte subsets include: CD3 count, CD4 count, CD8 count, CD4:CD8 ratio, CD19 count & CD56 count |
| Request Group | FLOW | TSUB & LSUB |
20th July 2021 | Processing Instructions | Prefer EDTA sample.
Do not spin, store at room temperature. | Prefer EDTA sample.
Do not spin, store at room temperature. |
20th July 2021 | Notes | Used to monitor T-cell numbers
Preferred EDTA sample. Do not spin, store at room temperature. | Used to monitor T-cell numbers
Preferred EDTA sample. Do not spin, store at room temperature. |
20th July 2021 | Collection & Request Instructions |
The samples are not stable for more than 24 hours. Hence all samples must be recieved in the laboratory at Box Hill Monday to Friday within business hours. | The samples are not stable for more than 24 hours. Hence all samples must be recieved in the laboratory at Box Hill Monday to Friday within business hours.
Cutoff for outpatient sample collection on Fridays is at 11.30 AM and MUST reach the laboratory before 4.00PM.
|
| Processing Instructions |
Prefer EDTA sample.
Do not spin, store at room temperature. | Prefer EDTA sample.
Do not spin, store at room temperature. |
| Notes |
Used to monitor T-cell numbers
Preferred EDTA sample. Do not spin, store at room temperature. | Used to monitor T-cell numbers
Preferred EDTA sample. Do not spin, store at room temperature. |
Recent changes for Measles Serology
Recent changes for Measles Serology |
Date | Field | Changed From | Changed To |
18th January 2023 | Laboratory | Microbiology | Biochemistry |
| Request Group | SMEA | MEA |
| Frequency | Daily | Twice weekly |
Recent changes for Methotrexate
Recent changes for Methotrexate |
Date | Field | Changed From | Changed To |
1st February 2023 | Laboratory | Referred Test | Biochemistry |
| Collection & Request Instructions | Send ASAP (within 24 hrs, 7 days a week). Contact reference laboratory prior to sending the sample. | Testing performed on an as needs basis. |
| External Laboratory | Peter MacCallum Cancer Institute | |
| Method | | Hand deliover samples to Biochemistry DO NOT load onto the 8100 |
Recent changes for Mumps Serology
Recent changes for Mumps Serology |
Date | Field | Changed From | Changed To |
18th January 2023 | Laboratory | Microbiology | Biochemistry |
| Request Group | SMUM | MUM |
| Frequency | Daily | Twice weekly |
Recent changes for Muscle Biopsy
Recent changes for Muscle Biopsy |
Date | Field | Changed From | Changed To |
30th December 2022 | Collection & Request Instructions | Note: This is an URGENT send out test.
Laboratory hours are 9am - 3pm, Monday - Friday.
| Note: This is an URGENT send out test.
Laboratory hours are 9am - 3pm, Monday - Friday.
Specimen must arrive at Alfred Pathology by 4pm.
|
| External Transport Instructions | Place the specimen in an esky with ice cubes and dispatch immediately. | Place the specimen in an esky with ice cubes and dispatch immediately. Must arrive at Alfred by 4pm. |
30th December 2022 | Collection & Request Instructions | Note: This is an URGENT send out test.
Laboratory hours are 9am - 5pm, Monday - Friday.
| Note: This is an URGENT send out test.
Laboratory hours are 9am - 3pm, Monday - Friday.
|
Recent changes for Paroxysmal Nocturnal Haemoglobinuria
Recent changes for Paroxysmal Nocturnal Haemoglobinuria |
Date | Field | Changed From | Changed To |
15th October 2021 | Collection & Request Instructions | Separate specimen required.
Cutoff for outpatient sample collection on Fridays is at 11.30 AM and MUST reach the laboratory before 4.00PM. | Whole blood - 1 x EDTA sample, do not spin. Store at room temperature. An FBE and BLOOD FILM must also be requested, and needs a separate EDTA sample.
Cutoff for outpatient sample collection on Fridays is at 11.30 AM and MUST reach the laboratory before 4.00PM. |
12th October 2021 | Alternative names | CD 59, PNH flow cytometry, PNH screen | CD59, PNH screen flow cytometry, FLEAR |
12th October 2021 | Alternative names | CD 59, PNH screen | CD 59, PNH screen, PNH flow cytometry |
| Request Group | FLOW | PNHS |
20th July 2021 | Collection & Request Instructions |
Separate specimen required. | Separate specimen required.
Cutoff for outpatient sample collection on Fridays is at 11.30 AM and MUST reach the laboratory before 4.00PM. |
Recent changes for Procalcitonin
Recent changes for Procalcitonin |
Date | Field | Changed From | Changed To |
17th November 2022 | Method | All PCT requests must be discussed with the Chemical Pathologist upon receipt in lab, regardless of time of day or day of week. If urgent/Taxi requiring is determined, result must be phoned to the clinician upon receipt - High Risk Result. | |
17th August 2022 | Method | | All PCT requests must be discussed with the Chemical Pathologist upon receipt in lab, regardless of time of day or day of week. If urgent/Taxi requiring is determined, result must be phoned to the clinician upon receipt - High Risk Result. |
27th July 2022 | Laboratory | Biochemistry | Referred Test |
| Volume (Adults) | | 0.5 mL |
| External Laboratory | | Royal Childrens Hospital |
Recent changes for Prothrombin Time
Recent changes for Q Fever Serology
Recent changes for Q Fever Serology |
Date | Field | Changed From | Changed To |
8th February 2023 | External Laboratory | Victorian Infectious Diseases Reference Laboratory (V.I.D.R.L) | |
Recent changes for Red Cell Antigen Genotyping
Recent changes for Red Cell Antigen Genotyping |
Date | Field | Changed From | Changed To |
9th May 2022 | Collection & Request Instructions |
Minimum volume of 4 ml required - this specimen cannot be used for any other purpose.
A completed "ARCBS Red Cell Reference Form" must accompany each sample sent for analysis.
This form, which can be completed online and subsequently printed to be sent with the sample can be found by clicking here | Minimum volume of 4 ml required - this specimen cannot be used for any other purpose.
A completed "ARCBS Red Cell Reference Form" must accompany each sample sent for analysis.
This form, which can be completed online and subsequently printed to be sent with the sample can be found by clicking here |
| Processing Instructions |
Specimen will arrive in department at RT. Please keep at 4 degrees prior to transport. | Specimen will arrive in department at RT. Please keep at 4 degrees prior to transport. |
| External Transport Instructions |
Transport at 4 degrees | Transport at 4 degrees |
Recent changes for Respiratory Virus PCR
Recent changes for Respiratory Virus PCR |
Date | Field | Changed From | Changed To |
14th February 2023 | Method | The following specimens will be referred for testing-
Sputum, Bronchial Washings, Bronchoalveolar Lavages - VIDRL
Swab specimens for Respiratory Multiplex PCR will be referred to Monash Molecular Laboratory
| The following specimens will be referred for testing-
Sputum, Bronchial Washings, Bronchoalveolar Lavages - VIDRL
As of Feb 2023 swab specimens for Respiratory Multiplex PCR will be tested in-house.
|
31st August 2022 | Preferred Specimen Type | Nasopharyngeal swab Nasopharyngeal Aspirate | Nasopharyngeal swab NTS - nose & throat swab Nasopharyngeal Aspirate |
| Optional Specimen Type | | Sputum Bronchial Washings Broncho-alveolar Lavage |
| Preferred Container Type | Viral Transport Media- Nasopharyngeal Respiratory aspiration pot | Viral Transport Media- Nasopharyngeal Respiratory aspiration pot respiratory PCR requirements |
| Collection & Request Instructions | Please note these swabs are available from stores.
Search for: VIRAL TRANSPORT MEDIA - NASOPHARYNGEAL
Pathology do not keep a stock of these swabs. Emergency departments and wards should manage a supply of these swabs according to demand.
| Please note these swabs are available from stores.
Pathology do not keep a stock of these swabs. Emergency departments and wards should manage a supply of these swabs according to demand.
|
| Method | All tests for respiratory virus PCR are considered urgent and must be delivered to the laboratory ASAP.
ICU/Oncology patients testing negative for Flu A/B and RSV will be referred to Monash for respiratory virus panel. This includes Influenza A virus, Influenza B virus, Respiratory Syncitial Virus, Picornavirus (RV/EV), Parainfluenza viruses 1,2 and 3, Adenovirus, Human Metapneumovirus, Bordetella pertussis, Bordetella parapertussis, Mycoplasma pneumoniae, Legionella species, Chlamydiaceae.
The following specimens will be referred for testing-
Sputum, Bronchial Washings, Bronchoalveolar Lavages
Specimens for Respiratory Multiplex PCR will be referred to Monash Molecular Laboratory
Contact Monash Pathology 9594 4532 or if no answer try Bacteriology 9594 4565 as there is someone rostered 24/7
| The following specimens will be referred for testing-
Sputum, Bronchial Washings, Bronchoalveolar Lavages - VIDRL
Swab specimens for Respiratory Multiplex PCR will be referred to Monash Molecular Laboratory
|
| Notes | This PCR will detect and identify: Influenza A virus, Influenza B virus and Respiratory syncytial virus (RSV).
Transport at room temperature to the Laboratory
Testing is performed at Box Hill, Angliss, and Maroondah.
Testing Lab
|
Testing hours
|
TAT (from receipt in lab)
|
All sites
|
Available 24/7
|
1 hr
|
Samples received after hours may have a longer TAT due to other urgent critical pathology testing occurring. | This PCR will detect and identify: Influenza A virus, Influenza B virus and Respiratory syncytial virus (RSV).
Transport at room temperature to the Laboratory
Testing is performed at Box Hill, Angliss, and Maroondah.
Testing Lab
|
Testing hours
|
TAT (from receipt in lab)
|
All sites
|
Available 24/7
|
6-12 hrs routine
2 hours (rapid approval form required)
|
DO NOT send samples in the Pathology Chute
For Rapid testing from Emergency Department - swab must be accompanied by signed Rapid Test Authorisation Form.
DO NOT call the laboratory for results/enquiries. Inpatient tests will be prioritised and results available on EMR ASAP.
Sputum samples are referred to an external laboratory - results will be delayed. |
Recent changes for Respiratory Virus PCR - Extended
Recent changes for Respiratory Virus PCR - Extended |
Date | Field | Changed From | Changed To |
14th February 2023 | Preferred Container Type | Viral Transport Media - Nasopharyngeal | Viral Transport Media - Nasopharyngeal respiratory PCR requirements Viral Transport Media- Nasopharyngeal |
14th February 2023 | Preferred Specimen Type | NTS - nose & throat swab Swab | NTS - nose & throat swab Respiratory samples |
Recent changes for Rickettsia spp. Screen Serology
Recent changes for Rickettsia spp. Screen Serology |
Date | Field | Changed From | Changed To |
8th February 2023 | External Laboratory | Victorian Infectious Diseases Reference Laboratory (V.I.D.R.L) | |
Recent changes for Ristocetin Co-Factor assay
Recent changes for Ristocetin Co-Factor assay |
Date | Field | Changed From | Changed To |
11th June 2021 | Alternative names | RICOF | RICOF, vWF:Activity, vWF:Act |
| Laboratory | Referred Test | Coagulation |
| External Laboratory | Austin Pathology | |
Recent changes for Scabies investigation
Recent changes for Scabies investigation |
Date | Field | Changed From | Changed To |
8th June 2021 | Collection & Request Instructions | Scabies collection instructions
Preparation
Wash the area of skin to be scraped with an alcohol swab.
Remove any ointment or residual applications using soap and water.
Disinfect the area with a skin cleansing alcohol wipe and allow it to dry.
Abrade the infested area with a sterile scalpel to the depth of pinpoint bleeding.
Transfer skin scrapings to a sterile yellow top pot for transport to the laboratory. Do NOT include the scalpel – dispose of in sharps bin
Label pot with patient name, UR No and DOB, date of collection, site of collection | Preparation
Wash the area of skin to be scraped with an alcohol swab.
Remove any ointment or residual applications using soap and water.
Disinfect the area with a skin cleansing alcohol wipe and allow it to dry.
Abrade the infested area with a sterile scalpel to the depth of pinpoint bleeding.
Transfer skin scrapings to a sterile yellow top pot for transport to the laboratory. Do NOT include the scalpel – dispose of in sharps bin
Label pot with patient name, UR No and DOB, date of collection, site of collection |
Recent changes for Thiopurine Metabolites
Recent changes for Thiopurine Metabolites |
Date | Field | Changed From | Changed To |
3rd March 2023 | Preferred Container Type | Lithium heparin no gel EDTA | See Instructions |
25th August 2022 | Collection & Request Instructions | Collect 1 x 4 ml Lithium Heparin tube and
1 x 4ml EDTA tube.
FBE must be requested with this test as a RCC is required for calculation of results
DO NOT SPIN.
Keep at Room Temp.
| Collect 1 x 4 ml Lithium Heparin tube and
2 x 4ml EDTA tube.
FBE must be requested with this test as a RCC is required for calculation of results
DO NOT SPIN.
Keep at Room Temp.
|
23rd August 2022 | Collection & Request Instructions | Collect 1 x 4 ml Lithium Heparin tube and 1 x 4ml EDTA tube.
FBE must be requested with this test as a RCC is required for calculation of results
DO NOT SPIN.
Keep at Room Temp.
| Collect 1 x 4 ml Lithium Heparin tube and
1 x 4ml EDTA tube.
FBE must be requested with this test as a RCC is required for calculation of results
DO NOT SPIN.
Keep at Room Temp.
|
23rd August 2022 | Request Group | | TMETAB |
| Preferred Container Type | EDTA | Lithium heparin no gel EDTA |
| Collection & Request Instructions | | Transport specimen at Room Temperature to lab |
| Collection & Request Instructions | Collect 2x4ml EDTA blood. DO NOT SPIN
Keep at 4 deg C, do not freeze.
The metabolites are stable for upto 4 days at 4 degress. | Collect 1 x 4 ml Lithium Heparin tube and 1 x 4ml EDTA tube.
FBE must be requested with this test as a RCC is required for calculation of results
DO NOT SPIN.
Keep at Room Temp.
|
| Processing Instructions | | Keep samples at Room Temperature.
Do Not Spin. Whole Blood required |
| External Laboratory | | St Vincents Hospital |
| Frequency | | Weekly |
| Notes | Imuran is a trade name for azathioprine
The Thiopurine metabolites (6-TGN & 6-MMP) are used as a means of estimating the toxicity and efficacy of azathioprine treatment.
This test is performed by the Gastroenerology department. ( Phone No.9895 4632 )
The cost for the test is not covered by Medicare.
Eastern Health patients:6TGN 6MMP Thiopurine Metabolites Testing $75
All other patients: 6TGN 6MMP Thiopurine Metabolites Testing $145 | Imuran is a trade name for azathioprine
The Thiopurine metabolites (6-TGN & 6-MMP) are used as a means of estimating the toxicity and efficacy of azathioprine treatment.
|
Recent changes for Tissue release from Anatomical Pathology
Recent changes for Toxoplasma serology
Recent changes for Toxoplasma serology |
Date | Field | Changed From | Changed To |
18th January 2023 | Request Group | STOX | TOX |
Recent changes for Transthyretin
Recent changes for Transthyretin |
Date | Field | Changed From | Changed To |
13th September 2022 | Processing Instructions |
The serum must be aliquoted and frozen. Transthyretin is only stable for 3 days at 4-8C . | The serum must be aliquoted and frozen. Transthyretin is only stable for 3 days at 4-8C . |
| External Laboratory | Monash Pathology | |
Recent changes for Typhoid
Recent changes for Typhoid |
Date | Field | Changed From | Changed To |
31st January 2023 | Alternative names | enteric fever, Typhoid fever | enteric fever, typhoid fever |
31st January 2023 | Preferred Container Type | Blood Culture | Blood Culture (Adult) Blood Culture (Paediatric) |
Recent changes for Vitamin B1
Recent changes for Vitamin B1 |
Date | Field | Changed From | Changed To |
18th November 2022 | External Laboratory | Australian Clinical Labs (formerly Healthscope) | St Vincents Hospital |
Recent changes for Vitamin B6
Recent changes for Vitamin B6 |
Date | Field | Changed From | Changed To |
18th November 2022 | External Laboratory | Australian Clinical Labs (formerly Healthscope) | St Vincents Hospital |
Recent changes for Vitamin C
Recent changes for Vitamin C |
Date | Field | Changed From | Changed To |
31st March 2022 | Volume (Adults) | 2 mL | 1 mL |
| Minimum/Paediatric Volume | | 1 mL |
| Processing Instructions | Spin, separate, wrap in foil and freeze ASAP.
Transport frozen. Contact Gribbles to arrange courier. | Spin, separate, wrap in foil and freeze ASAP.
Transport frozen. |
| External Laboratory | Australian Clinical Labs (formerly Healthscope) | Alfred Hospital |
| Frequency | Weekly | Fortnightly |
Recent changes for Xanthochromia
Recent changes for Xanthochromia |
Date | Field | Changed From | Changed To |
14th July 2021 | Laboratory | Referred Test | Biochemistry |
| Collection & Request Instructions | Please note : to avoid sample deterioration, DO NOT send CSF specimens via the vacuum chute system.
Please quickly check that the tube is labelled underneath the foil before referring.
THERE MUST BE A MINIMUM OF 0.6 mL OF CSF OR THE TEST CANNOT BE PERFORMED
| Please note : to avoid sample deterioration, DO NOT send CSF specimens via the vacuum chute system.
THERE MUST BE A MINIMUM OF 0.6 mL OF CSF OR THE TEST CANNOT BE PERFORMED
|
| Processing Instructions | CSF xanthochromia checklist
Sample collected >12hrs from onset symptoms Preferable
4th CSF sample Essential
Sample light protected immediately Essential
Sample transported manually to lab Essential
Serum sample for bilirubin & albumin Preferable
Slip with clinical notes and contact details Essential
| CSF xanthochromia checklist
Sample collected >12hrs from onset symptoms Preferable
4th CSF sample Essential
Sample light protected immediately Essential
Sample transported manually to lab Essential
Serum sample for bilirubin & protein- essemtial
Slip with clinical notes and contact details Essential
|
| External Laboratory | Austin Pathology | |
| External Transport Instructions | Send via a Taxi ASAP. Ring Austin Pathology on 94963100. | MH and AH send via taxi ASAP and notify BH laboraotry. |
| Frequency | Daily | As required |
| Method | | Please quickly check that the tube is labelled underneath the foil before passing to Scientist.
The specimen designated for spectrophotometry should be centrifuged at .2000 rpm for 5 min as soon as possible after receipt in the laboratory. The supernatant must be stored in the dark at 40 C until analysis. |
| Notes | This test is specialised and will only be performed on CSF's when:
CT scan is negative
Four tubes must be collected and the container of the 4th tube must be wrapped in foil (please Label specimen before wrapping in foil) | This test is specialised and will only be performed on CSF's when:
CT scan is negative
Four tubes must be collected and the container of the 4th tube must be wrapped in foil (please Label specimen before wrapping in foil)
A white top tube must be collected for simulataneous measurement of serum Bilirubin and serum Protein (or add on to existing bloods within 6 hours of CSF collection). |
Get Connected