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Recent changes for Aldosterone - urine
Recent changes for Aldosterone - urine |
Date | Field | Changed From | Changed To |
4th February 2019 | Preferred Container Type | 24 hour urine | 24-hour urine - ACID bottle |
| Collection & Request Instructions | 24-hour urine collection in plain bottle
Keep refrigerated during collection and send to laboratory immediately on completion
Patient Collection Instructions | 24-hour urine collection in acid bottle
Keep refrigerated during collection and send to laboratory immediately on completion
Patient Collection Instructions |
| Method | 24 hour urine, plain bottle. A 50 mL aliquot is sent with the recorded total 24 hrs volume.
An extra 50 mL aliquot needs to be stored. | 24 hour urine, acid bottle. A 50 mL aliquot is sent with the recorded total 24 hrs volume.
An extra 50 mL aliquot needs to be stored. |
Recent changes for Alpha Foetoprotein
Recent changes for Alpha Foetoprotein |
Date | Field | Changed From | Changed To |
17th January 2020 | Collection & Request Instructions | Clinical notes must be completed
If pregnant female, please state pregancy in weeks
AFP is useful in monitoring some testicular germ cell tumours and primary hepatocelluar carcnioma. After treatment, eg. orchidectomy, levels fall with a half-life of approximately 45 days. Values > 500 mg/L are suggestive of malignancy
However 50% of all malignancies have values < 500 mg/L. AFP can also be raised in a variety of non-malignant liver disorders, where levels are usually < 100 mg/L. | Clinical notes must be completed
If pregnant female, please state pregancy in weeks
AFP is useful in monitoring some testicular germ cell tumours and primary hepatocelluar carcnioma. After treatment, eg. orchidectomy, levels fall with a half-life of approximately 45 days. Values > 500 ug/L are suggestive of malignancy
However 50% of all malignancies have values < 500 ug/L. AFP can also be raised in a variety of non-malignant liver disorders, where levels are usually < 100 ug/L. |
Recent changes for Alpha-1 Antitrypsin - phenotyping
Recent changes for Alpha-1 Antitrypsin - phenotyping |
Date | Field | Changed From | Changed To |
18th June 2019 | Frequency | | Monthly |
Recent changes for Aluminium
Recent changes for Aluminium |
Date | Field | Changed From | Changed To |
12th February 2020 | Collection & Request Instructions |
LAB STAFF NOTE : The Reference Lab will only receive aliquots which are sent in a metal-free aliquot tube (see container picture above). Sample must be decanted by pour-off (do not use plastic pipettes). |
LAB STAFF NOTE : The Reference Lab will only receive aliquots which are sent in a metal-free aliquot tube (see container picture above). Sample must be decanted by pour-off (do not use plastic pipettes). |
7th February 2020 | Collection & Request Instructions | The Reference Lab will only receive aliquots which are sent in a metal-free aliquot tube (see container picture above). Sample must be decanted by pour-off (do not use plastic pipettes). |
LAB STAFF NOTE : The Reference Lab will only receive aliquots which are sent in a metal-free aliquot tube (see container picture above). Sample must be decanted by pour-off (do not use plastic pipettes). |
7th February 2020 | Preferred Container Type | Trace metal tube ( Dark blue ) | Trace Metal with Aliquot |
| Collection & Request Instructions | Do Not spin - Transport at 40C | The Reference Lab will only receive aliquots which are sent in a metal-free aliquot tube (see container picture above). Sample must be decanted by pour-off (do not use plastic pipettes). |
Recent changes for Amiodarone
Recent changes for Amiodarone |
Date | Field | Changed From | Changed To |
13th February 2019 | Collection & Request Instructions |
Pre-dose serum is required.
Trough level - collect at least 6-8 hours after last dose. | Trough level - collect at least 6-8 hours after last dose.
|
| Processing Instructions |
Desethylamiodarone is the major metabolite of Amiodarone | Spin, aliquot and freeze plasma. |
| External Laboratory | St Vincents Hospital | Royal Brisbane & Womens Hospital |
Recent changes for Angiotensin Converting Enzyme
Recent changes for Angiotensin Converting Enzyme |
Date | Field | Changed From | Changed To |
25th March 2019 | Collection & Request Instructions | | Transport ON ICE IMMEDIATELY to lab |
| Collection & Request Instructions |
| Preferable to receive a fasting sample.
Transport to laboratory on ice or transport immediately to laboratory and contact the laboratory staff to expect the sample.
|
Recent changes for Anti-Mitochondrial Antibodies
Recent changes for Anti-Mitochondrial Antibodies |
Date | Field | Changed From | Changed To |
22nd February 2019 | Alternative names | Mitochondrial Antibodies | Mitochondrial Antibodies, AMA |
Recent changes for Anti-Thrombin III
Recent changes for Anti-Thrombin III |
Date | Field | Changed From | Changed To |
19th January 2021 | Collection & Request Instructions | Fill to label marker and mix by inversion.
DO NOT use 1 mL citrate tubes.
1 x 3.5 ml citrate tubes sufficient for Protein C, Protein S and Antithrombin 3. | Fill to label marker and mix by inversion.
DO NOT use 1 mL citrate tubes.
Samples must be transported to laboratory immediately. ( Within a maximun of 4 hours from collection time)
1 x 3.5 ml citrate tubes sufficient for Protein C, Protein S and Antithrombin 3. |
10th December 2020 | Collection & Request Instructions | Fill to label marker and mix by inversion.
DO NOT use 1 mL citrate tubes. | Fill to label marker and mix by inversion.
DO NOT use 1 mL citrate tubes.
1 x 3.5 ml citrate tubes sufficient for Protein C, Protein S and Antithrombin 3. |
Recent changes for Beta - 2-Transferrin, Asialo - Fluid
Recent changes for Beta - 2-Transferrin, Asialo - Fluid |
Date | Field | Changed From | Changed To |
19th May 2020 | Volume (Adults) | | 1.0 mL |
| Minimum/Paediatric Volume | | 0.2 mL |
| Collection & Request Instructions | | Wicks and Gauze will not be accepted. |
| Notes | The presence of asialotransferrin in a fluid indicates that it is, or contains, CSF. | The presence of asialotransferrin in a fluid indicates that it is, or contains, CSF.
Pathologist approval required. |
Recent changes for Blood Cultures - Adult
Recent changes for Blood Cultures - Children and Neonates
Recent changes for C-Reactive Protein (high sensitive)
Recent changes for C-Reactive Protein (high sensitive) |
Date | Field | Changed From | Changed To |
12th July 2019 | Laboratory | Blood Bank | Referred Test |
| Processing Instructions | CRP-hs is run on a weekly basis in the Box Hill lab. Specimens should be frozen at -20 within 24 hours of collection. | |
| External Laboratory | | St Vincents Hospital |
| External Transport Instructions | If transporting within 24 hours of collection, 4 deg transport is acceptable. Otherwise, aliquot should be frozen. | |
| Frequency | Weekly | Daily |
Recent changes for CADASIL-Notch 3 gene mutation
Recent changes for CADASIL-Notch 3 gene mutation |
Date | Field | Changed From | Changed To |
7th November 2019 | External Laboratory | Neurogenetics Unit, Level 2, Royal Perth Hospital | Pathwest Lab Med WA |
| Frequency | | As required |
| Method |
Packed by certified personnel
Dr M Davis, Neurogenetics Unit, DPT of Anatomical Pathology, Level 2, North Block, RPA, Wellington Street, PERTH, WA 6000
(Ph) 08-92243136. | Packed by certified personnel
Dr M Davis, Diagnostic Genomics, Level 2
C/O Specimen Reception Ground Floor PP Building
QEII Medical Centre Hospital Avenue
Nedlands WA 6009 AUSTRALIA
(Ph) 08-63834243 |
| Notes |
Defects in NOTCH3 are the cause of cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL) . CADASIL causes a type of stroke and dementia of which key features include recurrent subcortical ischemic events and vascular dementia. The disorder affects relatively young adults of both sexes. Mutations affect highly conserved cysteine residues within epidermal growth factor (EGF)-like repeat domains in the extracellular part of the receptor. | Defects in NOTCH3 are the cause of cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL) . CADASIL causes a type of stroke and dementia of which key features include recurrent subcortical ischemic events and vascular dementia. The disorder affects relatively young adults of both sexes. Mutations affect highly conserved cysteine residues within epidermal growth factor (EGF)-like repeat domains in the extracellular part of the receptor. |
Recent changes for Cadmium
Recent changes for Cadmium |
Date | Field | Changed From | Changed To |
12th February 2020 | Preferred Container Type | Trace Metal with Aliquot | Trace metal tube ( Dark blue ) |
| Notes | Transport specimen at Room Temperature to lab
Sample haemolysis may make this test unreportable
LAB STAFF NOTE : The Reference Lab will only receive aliquots which are sent in a metal-free aliquot tube (see container picture above). Sample must be decanted by pour-off (do not use plastic pipettes). | Transport specimen at Room Temperature to lab
Sample haemolysis may make this test unreportable
|
Recent changes for Candida auris screen
Recent changes for Candida auris screen |
Date | Field | Changed From | Changed To |
15th November 2019 | Alternative names | Candida screen | Candida screen, Candida auris |
Recent changes for Cerebrospinal Fluid - Biochemistry
Recent changes for Cerebrospinal Fluid - Biochemistry |
Date | Field | Changed From | Changed To |
14th August 2020 | Collection & Request Instructions | Please note : to avoid sample deterioration, DO NOT send CSF specimens via the vacuum chute system.
Depending on the tests requested, 3-4 separate tubes will need to be provided. Note that the tubes are numbered so that order of collection can be identified – this order should be carefully followed, so that the optimal sample can be used for the requested tests. It is also important to consider the tests that are required, as this will determine how much CSF is needed in each tube. See the matrix below for details.
TUBE 1 is used for Cytology.
TUBE 2 is used for Glucose, Protein and Flow Cytometry.
(centrifuged in the lab – the supernatant is used for Biochemistry analysis (glucose, protein) and the cellular pellet will be used for flow cytometry if required).
TUBE 3 is for Microbiological analysis (cell count, Gram stain and culture) and any further infectious testing (e.g. viral PCR, mycobacterial PCR and culture, cryptococcal antigen).
TUBE 4 will be required only for;
(a) Creutzfeld-Jacob Disease (CJD) testing OR
(b) Xanthochromia analysis (wrap the tube in foil to prevent sample deterioration due to light).
TESTS REQUIRED
|
MINIMUM REQUIRED VOLUME (mL)
|
TUBE 1
|
TUBE 2
|
TUBE 3
|
TUBE 4
|
CYTOLOGY. CHEMISTRY, MICRO/CULTURE
|
1.0-2.0
|
1.0
|
1.0-2.0
|
Nil
|
ADD FLOW CYTOMETRY
|
|
add 5.0-7.0
|
|
|
ADD OTHER INFECTIOUS MARKERS
|
|
|
add 2.0- 5.0-
|
|
ADD MYCOBACTERIAL CULTURE
|
|
|
Add 10.00
|
|
ADD CJD
|
|
|
|
1.0
|
ADD XANTHOCHROMIA
|
|
|
|
1.0 (foil)
|
Note, the ability to add further tests to a previously collected sample is dependent upon the volume provided. Also, many tests (flow cytometry, cytology) can only be performed on fresh specimens.
Other specialised testing (e.g. oligoclonal bands) will be performed using the supplied samples.
Xanthochromia and CJD analysis cannot be performed on a blood-stained sample. | Please note : to avoid sample deterioration, DO NOT send CSF specimens via the vacuum chute system.
Depending on the tests requested, 3-4 separate tubes will need to be provided. Note that the tubes are numbered so that order of collection can be identified – this order should be carefully followed, so that the optimal sample can be used for the requested tests. It is also important to consider the tests that are required, as this will determine how much CSF is needed in each tube. See the matrix below for details.
TUBE 1 is used for Cytology.
TUBE 2 is used for Glucose, Protein and Flow Cytometry.
(centrifuged in the lab – the supernatant is used for Biochemistry analysis (glucose, protein) and the cellular pellet will be used for flow cytometry if required).
TUBE 3 is for Microbiological analysis (cell count, Gram stain and culture) and any further infectious testing (e.g. viral PCR, mycobacterial PCR and culture, cryptococcal antigen).
TUBE 4 will be required only for;
(a) Creutzfeld-Jacob Disease (CJD) testing OR
(b) Xanthochromia analysis (wrap the tube in foil to prevent sample deterioration due to light).
TESTS REQUIRED
|
MINIMUM REQUIRED VOLUME (mL)
|
TUBE 1
|
TUBE 2
|
TUBE 3
|
TUBE 4
|
CYTOLOGY. CHEMISTRY, MICRO/CULTURE
|
1.0-2.0
|
1.0
|
1.0-2.0
|
Nil
|
ADD FLOW CYTOMETRY
|
|
add 5.0-7.0
|
|
|
ADD OTHER INFECTIOUS MARKERS
|
|
|
add 2.0- 5.0-
|
|
ADD MYCOBACTERIAL CULTURE
|
|
|
Add 10.00
|
|
ADD CJD
|
|
|
|
1.0
|
ADD XANTHOCHROMIA
|
|
|
|
1.0 (foil)
|
Note, the ability to add further tests to a previously collected sample is dependent upon the volume provided. Also, many tests (flow cytometry, cytology) can only be performed on fresh specimens.
Other specialised testing (e.g. oligoclonal bands) will be performed using the supplied samples.
Xanthochromia and CJD analysis cannot be performed on a blood-stained sample.
CSF Information - Laboratory Processing |
14th August 2020 | Collection & Request Instructions | Please note : to avoid sample deterioration, DO NOT send CSF specimens via the vacuum chute system.
If additional tests required eg. CSF oligoclonal bands, a minimum of 1.0 ml is required.
Collect CSF into three separate, sterile, screw-capped tubes provided (labelled 1,2 & 3)
Tube 1 is for Biochemistry (for Protein and Glucose) 0.5ml (10 drops) required
Tube 2 for Cytology 1.0ml (20 drops) and/or Flow Cytometry (6ml)
For Flow Cytometry a minimum of 6ml is required in Tube 2
For Flow Cytometry and Cytology a minimum of 7ml is required in Tube 2
Tube 3 for Microbiology upto 10ml (Full tube)
If querying CJD the laboratory requires a 4th unopened tube containing at least 1ml of non-bloodstained CSF.
Xanthochromia investigation requires a 4th tube containing 1ml of CSF . | Please note : to avoid sample deterioration, DO NOT send CSF specimens via the vacuum chute system.
Depending on the tests requested, 3-4 separate tubes will need to be provided. Note that the tubes are numbered so that order of collection can be identified – this order should be carefully followed, so that the optimal sample can be used for the requested tests. It is also important to consider the tests that are required, as this will determine how much CSF is needed in each tube. See the matrix below for details.
TUBE 1 is used for Cytology.
TUBE 2 is used for Glucose, Protein and Flow Cytometry.
(centrifuged in the lab – the supernatant is used for Biochemistry analysis (glucose, protein) and the cellular pellet will be used for flow cytometry if required).
TUBE 3 is for Microbiological analysis (cell count, Gram stain and culture) and any further infectious testing (e.g. viral PCR, mycobacterial PCR and culture, cryptococcal antigen).
TUBE 4 will be required only for;
(a) Creutzfeld-Jacob Disease (CJD) testing OR
(b) Xanthochromia analysis (wrap the tube in foil to prevent sample deterioration due to light).
TESTS REQUIRED
|
MINIMUM REQUIRED VOLUME (mL)
|
TUBE 1
|
TUBE 2
|
TUBE 3
|
TUBE 4
|
CYTOLOGY. CHEMISTRY, MICRO/CULTURE
|
1.0-2.0
|
1.0
|
1.0-2.0
|
Nil
|
ADD FLOW CYTOMETRY
|
|
add 5.0-7.0
|
|
|
ADD OTHER INFECTIOUS MARKERS
|
|
|
add 2.0- 5.0-
|
|
ADD MYCOBACTERIAL CULTURE
|
|
|
Add 10.00
|
|
ADD CJD
|
|
|
|
1.0
|
ADD XANTHOCHROMIA
|
|
|
|
1.0 (foil)
|
Note, the ability to add further tests to a previously collected sample is dependent upon the volume provided. Also, many tests (flow cytometry, cytology) can only be performed on fresh specimens.
Other specialised testing (e.g. oligoclonal bands) will be performed using the supplied samples.
Xanthochromia and CJD analysis cannot be performed on a blood-stained sample. |
Recent changes for Cerebrospinal Fluid - Cytology
Recent changes for Cerebrospinal Fluid - Cytology |
Date | Field | Changed From | Changed To |
14th August 2020 | Collection & Request Instructions | The sample with the least amount of blood is prefered for Cytology.
Collect CSF into three separate, sterile, screw-capped tubes provided (labelled 1,2 & 3)
Tube 1 is for Biochemistry (for Protein and Glucose) 0.5ml (10 drops) required
Tube 2 for Cytology 1.0ml (20 drops) and/or Flow Cytometry (6ml)
For Flow Cytometry a minimum of 6ml is required in Tube 2
For Flow Cytometry and Cytology a minimum of 7ml is required in Tube 2
Tube 3 for Microbiology upto 10ml (Full tube)
Xanthochromia investigation requires a 4th tube containing 1ml of CSF. | The sample with the least amount of blood is prefered for Cytology.
CSF SPECIMEN INFORMATION – LABORATORY PROCESSING
Depending on the tests requested, 3-4 separate tubes will need to be provided. Note that the tubes are numbered so that order of collection can be identified – this order should be carefully followed, so that the optimal sample can be used for the requested tests. It is also important to consider the tests that are required, as this will determine how much CSF is needed in each tube. See the matrix below for details.
TUBE 1 is used for Cytology.
TUBE 2 is used for Glucose, Protein and Flow Cytometry.
(centrifuged in the lab – the supernatant is used for Biochemistry analysis (glucose, protein) and the cellular pellet will be used for flow cytometry if required).
TUBE 3 is for Microbiological analysis (cell count, Gram stain and culture) and any further infectious testing (e.g. viral PCR, mycobacterial PCR and culture, cryptococcal antigen).
TUBE 4 will be required only for;
(a) Creutzfeld-Jacob Disease (CJD) testing OR
(b) Xanthochromia analysis (wrap the tube in foil to prevent sample deterioration due to light).
TESTS REQUIRED
|
MINIMUM REQUIRED VOLUME (mL)
|
TUBE 1
|
TUBE 2
|
TUBE 3
|
TUBE 4
|
CYTOLOGY. CHEMISTRY, MICRO/CULTURE
|
1.0-2.0
|
1.0
|
1.0-2.0
|
Nil
|
ADD FLOW CYTOMETRY
|
|
add 5.0-7.0
|
|
|
ADD OTHER INFECTIOUS MARKERS
|
|
|
add 2.0- 5.0-
|
|
ADD MYCOBACTERIAL CULTURE
|
|
|
Add 10.00
|
|
ADD CJD
|
|
|
|
1.0
|
ADD XANTHOCHROMIA
|
|
|
|
1.0 (foil)
|
Note, the ability to add further tests to a previously collected sample is dependent upon the volume provided. Also, many tests (flow cytometry, cytology) can only be performed on fresh specimens.
Other specialised testing (e.g. oligoclonal bands) will be performed using the supplied samples.
Xanthochromia and CJD analysis cannot be performed on a blood-stained sample. |
| Processing Instructions | Must be stored in the fridge.
| Must be stored in the fridge.
Out of hours CSF samples for routine testing can be stored in the AP refrigerator.
|
| Notes | Out of hours CSF samples for routine testing can be stored in the AP refrigerator.
|
|
31st May 2019 | Notes | out of hours CSF samples for cytology can be stored in the refrigerator for routine testing
Out of hours CSF samples for routine testing can be stored in the AP refrigerator.
| Out of hours CSF samples for routine testing can be stored in the AP refrigerator.
|
31st May 2019 | Collection & Request Instructions | CSF must be delivered to laboratory immediately; cellular degeneration occurs within 30 minutes.
The sample with the least amount of blood is prefered for Cytology.
Collect CSF into three separate, sterile, screw-capped tubes provided (labelled 1,2 & 3)
Tube 1 is for Biochemistry (for Protein and Glucose) 0.5ml (10 drops) required
Tube 2 for Cytology 1.0ml (20 drops) and/or Flow Cytometry (6ml)
For Flow Cytometry a minimum of 6ml is required in Tube 2
For Flow Cytometry and Cytology a minimum of 7ml is required in Tube 2
Tube 3 for Microbiology upto 10ml (Full tube)
Xanthochromia investigation requires a 4th tube containing 1ml of CSF . | The sample with the least amount of blood is prefered for Cytology.
Collect CSF into three separate, sterile, screw-capped tubes provided (labelled 1,2 & 3)
Tube 1 is for Biochemistry (for Protein and Glucose) 0.5ml (10 drops) required
Tube 2 for Cytology 1.0ml (20 drops) and/or Flow Cytometry (6ml)
For Flow Cytometry a minimum of 6ml is required in Tube 2
For Flow Cytometry and Cytology a minimum of 7ml is required in Tube 2
Tube 3 for Microbiology upto 10ml (Full tube)
Xanthochromia investigation requires a 4th tube containing 1ml of CSF. |
| External Transport Instructions | All out of hours specimens from Anglss and Maroondah should be sent to Box Hill ASAP for processing.
After hours cytology CSF can be prepared by microbiology staff. In the event microbiology staff are not in attendance and will not be called in, please call the Anatomical Pathology Scientist on call. | After hours cytology CSF can be prepared by microbiology staff. In the event microbiology staff are not in attendance please store in the AP fridge. |
| Method | CSF sample processing:
If Cytology/Flow Cytometry requested : Tube 1-Biochemistry, Tube 2- Cytology/Flow Cytometry, Tube 3-Microbiology
If Cytology/Flow Cytometry not requested: Tube 1 and 3-Microbiology, Tube 2-Biochemistry
Any confusion between the terms cell count and cytology could be cleared up when the cell count is rung through to the requesting doctor by the scientist. At this point it could be determined whether cytology is a genuine request or not (all to be documented in F8 notes). The CSF can then be forwarded expediently to BHH for cytospin if required.
| CSF sample processing:
If Cytology/Flow Cytometry requested : Tube 1-Biochemistry, Tube 2- Cytology/Flow Cytometry, Tube 3-Microbiology
If Cytology/Flow Cytometry not requested: Tube 1 and 3-Microbiology, Tube 2-Biochemistry
Any confusion between the terms cell count and cytology could be cleared up when the cell count is rung through to the requesting doctor by the scientist. At this point it could be determined whether cytology is a genuine request or not (all to be documented in F8 notes).
|
| Notes | | out of hours CSF samples for cytology can be stored in the refrigerator for routine testing
Out of hours CSF samples for routine testing can be stored in the AP refrigerator.
|
Recent changes for Cerebrospinal Fluid - microscopy and culture
Recent changes for Cerebrospinal Fluid - microscopy and culture |
Date | Field | Changed From | Changed To |
14th August 2020 | Notes | Special tests available on request:
PCR for viruses
Acid-fast stain and/or culture for Mycobacteria
Cryptococcal antigen
Serological test for Syphilis (must be paired with 5mL serum sample or test will not be performed)
Viral Serology, if CSF is accompanied by serum sample
Flow Cytometry - requires a 4th tube
The turnaround time for CSF microscopy are 30-40 minutes.
The turnaround time for CSF culture are 3-5 days. | CSF SPECIMEN INFORMATION – LABORATORY PROCESSING
Depending on the tests requested, 3-4 separate tubes will need to be provided. Note that the tubes are numbered so that order of collection can be identified – this order should be carefully followed, so that the optimal sample can be used for the requested tests. It is also important to consider the tests that are required, as this will determine how much CSF is needed in each tube. See the matrix below for details.
TUBE 1 is used for Cytology.
TUBE 2 is used for Glucose, Protein and Flow Cytometry.
(centrifuged in the lab – the supernatant is used for Biochemistry analysis (glucose, protein) and the cellular pellet will be used for flow cytometry if required).
TUBE 3 is for Microbiological analysis (cell count, Gram stain and culture) and any further infectious testing (e.g. viral PCR, mycobacterial PCR and culture, cryptococcal antigen).
TUBE 4 will be required only for;
(a) Creutzfeld-Jacob Disease (CJD) testing OR
(b) Xanthochromia analysis (wrap the tube in foil to prevent sample deterioration due to light).
TESTS REQUIRED
|
MINIMUM REQUIRED VOLUME (mL)
|
TUBE 1
|
TUBE 2
|
TUBE 3
|
TUBE 4
|
CYTOLOGY. CHEMISTRY, MICRO/CULTURE
|
1.0-2.0
|
1.0
|
1.0-2.0
|
Nil
|
ADD FLOW CYTOMETRY
|
|
add 5.0-7.0
|
|
|
ADD OTHER INFECTIOUS MARKERS
|
|
|
add 2.0- 5.0-
|
|
ADD MYCOBACTERIAL CULTURE
|
|
|
Add 10.00
|
|
ADD CJD
|
|
|
|
1.0
|
ADD XANTHOCHROMIA
|
|
|
|
1.0 (foil)
|
Note, the ability to add further tests to a previously collected sample is dependent upon the volume provided. Also, many tests (flow cytometry, cytology) can only be performed on fresh specimens.
Other specialised testing (e.g. oligoclonal bands) will be performed using the supplied samples.
Xanthochromia and CJD analysis cannot be performed on a blood-stained sample.
Special tests available on request:
PCR for viruses
Acid-fast stain and/or culture for Mycobacteria
Cryptococcal antigen
Serological test for Syphilis (must be paired with 5mL serum sample or test will not be performed)
Viral Serology, if CSF is accompanied by serum sample
Flow Cytometry - requires a 4th tube
The turnaround time for CSF microscopy are 30-40 minutes.
The turnaround time for CSF culture are 3-5 days. |
Recent changes for Cerebrospinal Fluid Specimen Collection
Recent changes for Cerebrospinal Fluid Specimen Collection |
Date | Field | Changed From | Changed To |
14th August 2020 | Notes | Collect CSF into three separate, sterile, screw-capped tubes provided (labelled 1,2 & 3)
Tube 1 is for Biochemistry (for Protein and Glucose) 0.5ml (10 drops) required
Tube 2 for Cytology 1.0ml (20 drops) and/or Flow Cytometry (6ml)
For Flow Cytometry a minimum of 6ml is required in Tube 2
For Flow Cytometry and Cytology a minimum of 7ml is required in Tube 2
Tube 3 for Microbiology upto 10ml (Full tube)
If querying CJD the laboratory requires a 4th unopened tube containing at least 1ml of non-bloodstained CSF.
Xanthochromia investigation requires a 4th tube containing 1ml of CSF .
Cell Reference Ranges
Adults:
0 - 5 lymphocytes (x10*6/L)
0 neutrophils
Neonates:
(£ 1 month)
0 – 20 white cells (x10*6/L, predominantly lymphocytes)
0 - several hundred erythrocytes
Leucocyte:erythrocyte (WBC:RBC) ratio is normally 1:500 (including traumatic tap).
| CSF SPECIMEN INFORMATION – LABORATORY PROCESSING
Depending on the tests requested, 3-4 separate tubes will need to be provided. Note that the tubes are numbered so that order of collection can be identified – this order should be carefully followed, so that the optimal sample can be used for the requested tests. It is also important to consider the tests that are required, as this will determine how much CSF is needed in each tube. See the matrix below for details.
TUBE 1 is used for Cytology.
TUBE 2 is used for Glucose, Protein and Flow Cytometry.
(centrifuged in the lab – the supernatant is used for Biochemistry analysis (glucose, protein) and the cellular pellet will be used for flow cytometry if required).
TUBE 3 is for Microbiological analysis (cell count, Gram stain and culture) and any further infectious testing (e.g. viral PCR, mycobacterial PCR and culture, cryptococcal antigen).
TUBE 4 will be required only for;
(a) Creutzfeld-Jacob Disease (CJD) testing OR
(b) Xanthochromia analysis (wrap the tube in foil to prevent sample deterioration due to light).
TESTS REQUIRED
|
MINIMUM REQUIRED VOLUME (mL)
|
TUBE 1
|
TUBE 2
|
TUBE 3
|
TUBE 4
|
CYTOLOGY. CHEMISTRY, MICRO/CULTURE
|
1.0-2.0
|
1.0
|
1.0-2.0
|
Nil
|
ADD FLOW CYTOMETRY
|
|
add 5.0-7.0
|
|
|
ADD OTHER INFECTIOUS MARKERS
|
|
|
add 2.0- 5.0-
|
|
ADD MYCOBACTERIAL CULTURE
|
|
|
Add 10.00
|
|
ADD CJD
|
|
|
|
1.0
|
ADD XANTHOCHROMIA
|
|
|
|
1.0 (foil)
|
Note, the ability to add further tests to a previously collected sample is dependent upon the volume provided. Also, many tests (flow cytometry, cytology) can only be performed on fresh specimens.
Other specialised testing (e.g. oligoclonal bands) will be performed using the supplied samples.
Xanthochromia and CJD analysis cannot be performed on a blood-stained sample. |
Recent changes for Chromium
Recent changes for Chromium |
Date | Field | Changed From | Changed To |
12th February 2020 | Preferred Container Type | Trace metal tube ( Dark blue ) | Trace Metal with Aliquot |
| Collection & Request Instructions | | LAB STAFF NOTE : The Reference Lab will only receive aliquots which are sent in a metal-free aliquot tube (see container picture above). Sample must be decanted by pour-off (do not use plastic pipettes). |
Recent changes for Coagulation Factor Assays II, V, VII, VIII, IX, X, XI, XII
Recent changes for Coagulation Factor Assays II, V, VII, VIII, IX, X, XI, XII |
Date | Field | Changed From | Changed To |
10th December 2020 | Collection & Request Instructions | Do not use 1.0 mL citrate tubes
Collect 1 X 3.5 ml Sodium Citrate tube is required to run all Factor assay ( Excent Factor XIII which is a refered test).
1 Tube is sufficient for performing these tests.
| Do not use 1.0 mL citrate tubes
Collect 1 X 3.5 ml Sodium Citrate tube is sufficient to run all Factor assay ( Excent Factor XIII which is a refered test).
1 Tube is sufficient for performing these tests.
|
10th December 2020 | Collection & Request Instructions | Do not use 1.0 mL citrate tubes | Do not use 1.0 mL citrate tubes
Collect 1 X 3.5 ml Sodium Citrate tube is required to run all Factor assay ( Excent Factor XIII which is a refered test).
1 Tube is sufficient for performing these tests.
|
Recent changes for Coagulation Studies
Recent changes for Coagulation Studies |
Date | Field | Changed From | Changed To |
10th April 2019 | Notes | When blood is collected from an arterial line it is necessary to discard the first 20 mL of blood to clear any heparin from the line before collecting the blood for coagulation tests
When collection is by vacuum tube, the coagulation tube should be collected second to avoid artefactual shortening of coagulation assays due to trauma of venesection
Whe blood is collected for an APTT or Anti-Xa Assay for control of heparin or low molecular weight heparin therapy, it is essential that the citrate tube is sent immediately to the laboratory
Tests include : INR, APTT
Do not use 1 ml citrate tubes. | When blood is collected from an arterial line it is necessary to discard the first 20 mL of blood to clear any heparin from the line before collecting the blood for coagulation tests
When collection is by vacuum tube, the coagulation tube should be collected second to avoid artefactual shortening of coagulation assays due to trauma of venesection
Whe blood is collected for an APTT or Anti-Xa Assay for control of heparin or low molecular weight heparin therapy, it is essential that the citrate tube is sent immediately to the laboratory
Do not use 1 ml citrate tubes. |
9th April 2019 | Request Group | COAG | COAGP |
Recent changes for Coronavirus PCR
Recent changes for Coronavirus PCR |
Date | Field | Changed From | Changed To |
24th January 2020 | Alternative names | extended panel PCR, MERS PCR, novel coronavirus PCR, Respiratory Virus NAD, SARS PCR | extended panel PCR, MERS PCR, novel coronavirus PCR, Respiratory Virus NAD, SARS PCR, novel Wuhan |
24th January 2020 | Alternative names | Respiratory Virus NAD, SARS PCR | Respiratory Virus NAD, SARS PCR, extended panel PCR, novel coronavirus PCR, MERS PCR |
| Request Group | RESPCR | RESPX |
Recent changes for COVID-19 Antibody
Recent changes for COVID-19 Antibody |
Date | Field | Changed From | Changed To |
24th September 2020 | Assay name | covab | COVAB |
| Alternative names | | SARS-CoV-2 Antibody
COVID Antibody
COVID Serology |
| Laboratory | Unassigned | Biochemistry |
| Request Group | | COVAB |
| Collection & Request Instructions | | Transport specimen at Room Temperature to lab |
| Frequency | | Daily |
Recent changes for COVID-19 PCR
Recent changes for COVID-19 PCR |
Date | Field | Changed From | Changed To |
16th September 2020 | External Laboratory | | Victorian Infectious Diseases Reference Laboratory (V.I.D.R.L) |
16th September 2020 | Collection & Request Instructions | Nasopharyngeal swab in RED Copan viral transport medium
Nasal/oropharyngeal swab in GREEN Kang Jian viral transport medium | Nasal/oropharyngeal swab in GREEN Kang Jian viral transport medium
Paediatric - Nasopharyngeal swab in RED Copan viral transport medium |
| Notes | For Rapid testing from Emergency Department - swab must be accompanied by signed Rapid Test Authorisation Form.
DO NOT call the laboratory for results/enquiries. Inpatient tests will be prioritised and results available on EMR ASAP.
Sputum samples are referred to an external laboratory - results will be delayed. | Samples MUST be in a clear specimen bag within an outer GREEN specimen bag
DO NOT send samples in the Pathology Chute
For Rapid testing from Emergency Department - swab must be accompanied by signed Rapid Test Authorisation Form.
DO NOT call the laboratory for results/enquiries. Inpatient tests will be prioritised and results available on EMR ASAP.
Sputum samples are referred to an external laboratory - results will be delayed. |
16th September 2020 | Preferred Specimen Type | Nasopharyngeal swab NTS - nose & throat swab | Nasopharyngeal swab NTS - nose & throat swab Sputum |
| Preferred Container Type | Viral Transport Media - Nasopharyngeal respiratory PCR requirements See Instructions | Viral Transport Media - Nasopharyngeal See Instructions Sterile Jar |
| Notes | For Rapid testing from Emergency Department - swab must be accompanied by signed Rapid Test Authorisation Form.
DO NOT call the laboratory for results/enquiries. Inpatient tests will be prioritised and results available on EMR. | For Rapid testing from Emergency Department - swab must be accompanied by signed Rapid Test Authorisation Form.
DO NOT call the laboratory for results/enquiries. Inpatient tests will be prioritised and results available on EMR ASAP.
Sputum samples are referred to an external laboratory - results will be delayed. |
Recent changes for Cyclosporin A
Recent changes for Cyclosporin A |
Date | Field | Changed From | Changed To |
6th May 2020 | Method | PLEASE NOTE : CYCLOSPORIN REFERRALS MUST ONLY BE SENT TO THE AUSTIN - THEY MUSY NOT BE SENT TO THE ALFRED AS THEY USE A METHOD THAT DOES NOT CORRESPOND WITH THE EXPECTED REFEERNCE RANGES. | PLEASE NOTE : CYCLOSPORIN send to the Austin Biochemistry Department. |
6th May 2020 | Collection & Request Instructions | In post transplant patients a 2 hour post dose is the preferred specimen, please indicate dosing information on the request slip.
Specimen must arrive at the Box Hill laboratory before 1200 Mon-Fri.
Separate EDTA specimen required.
| In post transplant patients a 2 hour post dose is the preferred specimen, please indicate dosing information on the request slip.
Specimen must arrive at the Box Hill laboratory before 1200 everyday.
Separate EDTA specimen required.
|
Recent changes for Disaccharidases, Maltase, Sucrase, Lactase and Isomaltase
Recent changes for Disaccharidases, Maltase, Sucrase, Lactase and Isomaltase |
Date | Field | Changed From | Changed To |
28th May 2019 | Processing Instructions | Place the biopsy specimen in the centre of a piece of Parafilm about 3cm square, and fold the Parafilm over the specimen so that it forms an envelope around the specimen. Then place this in a capped plastic tube with an appropriate label. Lab Notes Store and transport frozen. | THE TISSUE SAMPLE MUST BE FROZEN IMMEDIATELY AFTER COLLECTION, AS FOLLOWS.
1. WRAP (FOLD) THE TISSUE SAMPLE IN PARAFILM FIRST.
2. THEN WRAP THE SAMPLE IN ALFOIL.
3. FREEZE THE SPECIMEN ASAP.
4. SEND ON DRY ICE |
| External Laboratory | Royal Childrens Hospital | Sullivan Nicolaides Pathology ( Via Melb Path) |
Recent changes for DNA analysis for Thalassaemia (related to Hgb Electrophoresis)
Recent changes for DNA analysis for Thalassaemia (related to Hgb Electrophoresis) |
Date | Field | Changed From | Changed To |
10th December 2020 | Collection & Request Instructions | Send FBE, Iron studies and Hb electrophoresis results.
Send within 24 Hrs.
Results: 95943398 | Please collect 2x EDTA tube for this test.
Send FBE, Iron studies and Hb electrophoresis results.
Send within 24 Hrs.
Results: 95943398 |
Recent changes for Euglobulin Clot Lysis Time
Recent changes for Euglobulin Clot Lysis Time |
Date | Field | Changed From | Changed To |
10th December 2020 | Volume (Adults) | | |
| Method | | This test is no longer performed. Please contact Haematologist or Senior Scientist in Haematology if further information is required. |
Recent changes for Familial mediterranean genetic screen
Recent changes for Familial mediterranean genetic screen |
Date | Field | Changed From | Changed To |
11th October 2019 | Collection & Request Instructions |
Room temperature
This test is not covered by Medicare.
Cost for testing is $250
. | Room temperature
This test is not covered by Medicare.
Cost for testing is $300.
|
| Processing Instructions |
Interstate courier- Room Temperature
Department Of Molecular Genetics, Children's Hospital at Westmead, Loading Dock 5, Redbank Road, Northmead, NSW 2152.
For results Tel: 02-98453244 | Interstate courier- Room Temperature
Department Of Molecular Genetics, Children's Hospital at Westmead, Loading Dock 5, Redbank Road, Northmead, NSW 2152.
For results Tel: 02-98453244 |
Recent changes for Free PSA
Recent changes for Free PSA |
Date | Field | Changed From | Changed To |
27th August 2019 | Processing Instructions |
Frozen | Frozen |
| External Transport Instructions |
The Sample must be separated and Frozen. fPSA is only stable for 24 hrs Max. The total PSA must be performed before the chemical pathologist/senior scientist can determine if the request is appropriate. | The Sample must be separated and Frozen. fPSA is only stable for 24 hrs Max. The total PSA must be performed before the chemical pathologist/senior scientist can determine if the request is appropriate. |
Recent changes for Haemoglobin Analysis (including Electrophoresis)
Recent changes for Haemoglobin Analysis (including Electrophoresis) |
Date | Field | Changed From | Changed To |
10th December 2020 | Alternative names | Alkali Resistant Haemoglobin, Foetal Haemoglobin, Hb EPG, HbA2, HbF, HbH, HbS, Unstable Haemoglobin | Alkali Resistant Haemoglobin, Foetal Haemoglobin, Hb EPG, HbA2, HbF, HbH, HbS, Unstable Haemoglobin, Thalassaemia |
| Collection & Request Instructions | | Please collect 2 X EDTA Tube for this test. |
Recent changes for Heparin-Induced Thrombocytopenia Syndrome Screen
Recent changes for Heparin-Induced Thrombocytopenia Syndrome Screen |
Date | Field | Changed From | Changed To |
5th September 2019 | Laboratory | Blood Bank | Referred Test |
| Collection & Request Instructions | HITScreen/ELISA testing is only available after consultation with the Consultant Haematologist or Haematology Registrar. | HITScreen/ELISA testing (HITE) is only available after consultation with the Consultant Haematologist or Haematology Registrar. |
Recent changes for Hepatitis D Serology
Recent changes for Hepatitis D Serology |
Date | Field | Changed From | Changed To |
21st August 2019 | Request Group | HEPDAB & HEPDAG | HEPDAB |
Recent changes for Hereditary Amyloidosis
Recent changes for Hereditary Amyloidosis |
Date | Field | Changed From | Changed To |
22nd August 2019 | Alternative names | | TTR, FGA, APOA1, LYZ or Amyloidosis Gene |
| Volume (Adults) | | |
| Minimum/Paediatric Volume | | |
Recent changes for Herpes Virus PCR
Recent changes for Herpes Virus PCR |
Date | Field | Changed From | Changed To |
19th October 2020 | External Transport Instructions | Tests included in Monash PCR - Herpes simplex 1 virus, Herpes simplex 2 virus, Varicella zoster virus, Cytomegalovirus, Enterovirus (swabs only), Adenovirus (not faeces)
Specimens tested at Monash-
Swabs (dry or in UTM) serum, Plasma (ACD or EDTA), Sterile fluids, Respiratory samples, Urine.
Specimens anticoagulated with heparin are unsuitable as heparin inhibits PCR
For other specimens - refer to VIDRL. Tissue, CSF, saliva.
If CSF Herpes and Enterovirus PCR -send to VIDRL.
Refer to Referred Molecular Test Requests WORK-MI-90 | Tests included in Monash PCR - Herpes simplex 1 virus, Herpes simplex 2 virus, Varicella zoster virus, Cytomegalovirus, Enterovirus (swabs only), Adenovirus (not faeces)
Specimens tested at Monash-
Swabs (dry or in UTM), Sterile fluids, Respiratory samples, Urine.
Specimens anticoagulated with heparin are unsuitable as heparin inhibits PCR
For other specimens - refer to VIDRL. Tissue, CSF, saliva, serum, Plasma (ACD or EDTA),
If CSF Herpes and Enterovirus PCR -send to VIDRL.
Refer to Referred Molecular Test Requests WORK-MI-90 |
Recent changes for HMG-CoA Reductase
Recent changes for HMG-CoA Reductase |
Date | Field | Changed From | Changed To |
7th February 2020 | Alternative names | 3-Hydroxy-3-Methylglutaryl-coenzyme A Reductase), Anti-HMGCR, Autoantibodies to HMGCR | 3-Hydroxy-3-Methylglutaryl-coenzyme A Reductase, Anti-HMGCR, Autoantibodies to HMGCR |
Recent changes for Holotranscobalamin
Recent changes for Holotranscobalamin |
Date | Field | Changed From | Changed To |
24th February 2020 | Processing Instructions | HoloTC is only stable for 3 days at 2-8 C, hence if it is being stored at an Eastern Health Lab over the weekend, freeze and transport frozen. | |
| External Transport Instructions | Please send sample at 4 degrees C. for weekend storage please freeze. | |
| Frequency | | Daily |
12th February 2020 | Laboratory | Referred Test | Biochemistry |
| External Laboratory | Alfred Hospital | |
Recent changes for IgE, Total
Recent changes for IgE, Total |
Date | Field | Changed From | Changed To |
3rd December 2019 | Laboratory | Referred Test | Biochemistry |
| External Laboratory | St Vincents Hospital | |
Recent changes for Imatinib Level
Recent changes for Imatinib Level |
Date | Field | Changed From | Changed To |
4th April 2019 | Request Group | | IMAT |
14th February 2019 | Processing Instructions | | Spin,aliquot plasma and freeze. |
| External Transport Instructions | | Frozen sample to be packed by IATA trained personal. |
Recent changes for Isohaemagglutinin titre
Recent changes for Isohaemagglutinin titre |
Date | Field | Changed From | Changed To |
22nd May 2019 | Request Group | | ISOHGT |
| Processing Instructions |
A Blood Group and Antibody screen should be performed by Eastern Health Pathology in conjunction with this request ( in order to check for other antibodies and allow reporting of isohaemagglutinin titre ). Blood samples should be held and transported at 4 deg C. Samples can be held ( at 4 deg C ) for up to 3 days before dispatch. | A Blood Group and Antibody screen should be performed by Eastern Health Pathology in conjunction with this request ( in order to check for other antibodies and allow reporting of isohaemagglutinin titre ). Blood samples should be held and transported at 4 deg C. Samples can be held ( at 4 deg C ) for up to 3 days before dispatch. |
| Method |
Isohaemagglutinin titre may be requested in organ transplant patients as a base line test and at different times post transplant. The test refers to the titre of any of anti A and anti B in serum. Each individual produces different quantities of these antibodies. In Renal transplant patients ( given non ABO matched organs ) a rise from baseline isohaemagglutinin titre may prompt clinical intervention. | Isohaemagglutinin titre may be requested in organ transplant patients as a base line test and at different times post transplant. The test refers to the titre of any of anti A and anti B in serum. Each individual produces different quantities of these antibodies. In Renal transplant patients ( given non ABO matched organs ) a rise from baseline isohaemagglutinin titre may prompt clinical intervention. |
Recent changes for JAK2 Mutation Analysis
Recent changes for JAK2 Mutation Analysis |
Date | Field | Changed From | Changed To |
7th October 2019 | Collection & Request Instructions | Sample is stable at Room Temperature for at least 24 Hours.
JAK2 is for differentiation of reactive VS clonal myeloproliferative disorders.
Collect a separate sample for this test.
This test is not Medicare rebatable. (Cost $44.00 incl GST)
| Sample is stable at Room Temperature for at least 24 Hours.
JAK2 is for differentiation of reactive VS clonal myeloproliferative disorders.
Collect a separate sample for this test.
|
Recent changes for Leucocyte Lysomal Enzyme Analysis
Recent changes for Leucocyte Lysomal Enzyme Analysis |
Date | Field | Changed From | Changed To |
21st January 2020 | Notes | Room temperature.
Please note this test id not covered by Medicare Rebate - see 'Ordering' above. | Room temperature.
Please note this test is not covered by Medicare Rebate - see 'Ordering' above. |
21st January 2020 | Notes | Room temperature.
This test is not covered by Medicare, cost will be $432.00 as of March 2016. | Room temperature.
Please note this test id not covered by Medicare Rebate - see 'Ordering' above. |
21st January 2020 | Assay name | White Cell Enzymes | Leucocyte Lysomal Enzyme Analysis |
| Alternative names | Lysosomal Enzymes | White Cell Enzymes |
| Volume (Adults) | 4 mL | 8 mL |
| Minimum/Paediatric Volume | | 6 mL |
| Notes | Room temperature.
This test is not covered by Medicare, cost will be $474.00 as of March 2016. | Room temperature.
This test is not covered by Medicare, cost will be $432.00 as of March 2016. |
Recent changes for Lignocaine
Recent changes for Lignocaine |
Date | Field | Changed From | Changed To |
8th January 2020 | External Laboratory | Pacific Laboratory Medicine Services | Royal Brisbane & Womens Hospital |
| Frequency | Daily | As required |
Recent changes for Lupus Anticoagulant Screen
Recent changes for Lupus Anticoagulant Screen |
Date | Field | Changed From | Changed To |
19th January 2021 | Method | Centrifuge the two 3.5 mL citrate tubes and transfer the plasma to a labeled 4 mL plastic tube and re-centrifuge at room temperature (do not use the refrigerated centrifuge).
The double centrifuged plasma is transferred to two 1 mL eppendorf tubes and freeze.
Sample must be centrifuged within 4 hours of collection. | **Sample must be centrifuged within 4 hours of collection**
Centrifuge the two 3.5 mL citrate tubes (and transfer the plasma to a labeled 4 mL plastic tube and re-centrifuge at room temperature (do not use the refrigerated centrifuge). One sample is acceptable but 2 samples are preferable for Lupus testing.
The double centrifuged plasma is transferred into a new labelled tubes.
Double spun samples are then frozen in the -20'C freezer. |
19th January 2021 | Collection & Request Instructions | Notification of anticoagulant therapy is essential
One (1x) Sodium citrate tubes is required. Do not use 1 ml citrate tubes.
Filled to label marker and mixed by inversion
The patient must not be receiving heparin
Transport to laboratory immediately.
Tests performed:
INR, APTT, APTT Mix, Lupus sensitive APTT and Dilute Russell's Viper venom time (LA screen/Confirm).
| Notification of anticoagulant therapy is essential
One (1x) Sodium citrate tubes is required. Do not use 1 ml citrate tubes.
Filled to label marker and mixed by inversion
The patient must not be receiving heparin
Transport to laboratory immediately. (maximum of 4 hours from time of collection).
Tests performed:
INR, APTT, APTT Mix, Silica Clotting Time and Dilute Russell's Viper venom time (LA screen/Confirm).
|
| Method | Centrifuge the two 3.5 mL citrate tubes and transfer the plasma to a labeled 4 mL plastic tube and re-centrifuge at room temperature (do not use the refrigerated centrifuge).
The double centrifuged plasma is transferred to two 1 mL eppendorf tubes and freeze. | Centrifuge the two 3.5 mL citrate tubes and transfer the plasma to a labeled 4 mL plastic tube and re-centrifuge at room temperature (do not use the refrigerated centrifuge).
The double centrifuged plasma is transferred to two 1 mL eppendorf tubes and freeze.
Sample must be centrifuged within 4 hours of collection. |
10th December 2020 | Collection & Request Instructions | Notification of anticoagulant therapy is essential
Two (2x) Sodium citrate tubes required. Do not use 1 ml citrate tubes.
Filled to label marker and mixed by inversion
The patient must not be receiving heparin
Transport to laboratory immediately.
Tests performed:
INR, APTT, APTT Mix, Lupus sensitive APTT and Dilute Russell's Viper venom time (LA screen/Confirm).
| Notification of anticoagulant therapy is essential
One (1x) Sodium citrate tubes is required. Do not use 1 ml citrate tubes.
Filled to label marker and mixed by inversion
The patient must not be receiving heparin
Transport to laboratory immediately.
Tests performed:
INR, APTT, APTT Mix, Lupus sensitive APTT and Dilute Russell's Viper venom time (LA screen/Confirm).
|
Recent changes for MAG Antibody
Recent changes for MAG Antibody |
Date | Field | Changed From | Changed To |
31st January 2019 | Alternative names | | Anti-myelin associated glycoprotein antibodies |
| Request Group | MAGIFA | MAGAB |
| Frequency | | Weekly |
| Method |
Email Melb path before sending at sendaways.department@mps.com.au to advise the samples are coming. | Email Melb path before sending at sendaways.department@mps.com.au to advise the samples are coming. |
Recent changes for Mercury Urine
Recent changes for Mercury Urine |
Date | Field | Changed From | Changed To |
5th September 2019 | Request Group | UHG24 | UHG24/UHG |
| Collection & Request Instructions | Urine mercury concentration can be done on spot urine sample.
Patient Collection Instructions | Urine mercury concentration can be done on spot urine sample.(UHG)
Patient Collection Instructions |
30th August 2019 | Request Group | HGCRU | UHG24 |
| Volume (Adults) | | 50 mL |
| External Laboratory | Sydney South West Pathology Service(RPA) | Alfred Hospital |
| Frequency | | Daily |
Recent changes for Mumps Virus PCR
Recent changes for Mumps Virus PCR |
Date | Field | Changed From | Changed To |
3rd January 2020 | Preferred Container Type | Swab - red top, plain swab, wooden shaft Sterile Jar | Swab - red top, plain swab, wooden shaft Swab - blue top (Amies transport media) Swab - white top, plain swab, plastic shaft UTM/Sterile pot |
Recent changes for Muscle Biopsy
Recent changes for Muscle Biopsy |
Date | Field | Changed From | Changed To |
14th May 2019 | Preferred Container Type | Not required | Sterile Jar |
| Collection & Request Instructions | Please contact the laboratory in advance to ensure a muscle biopsy kit is readily available for collection.
Collection is as per kit instructions.
Note: This is an URGENT send out test.
Laboratory hours are 9am - 5pm, Monday - Friday.
| Note: This is an URGENT send out test.
Laboratory hours are 9am - 5pm, Monday - Friday.
|
| Processing Instructions | Call the Victorian Neuromuscular Laboratory for kits.
Contact: 9076 5448
Kits are to be stored in the fridge.
Prior to giving the kit to theatre staff, add 2mls of concentrated glutaraldehyde into 18mls of cacodylate buffer (red top container).
| Notify the Victorian Neuromuscular Laboratory.
Contact: 9076 5448
|
| External Transport Instructions | This is an URGENT send out test.
| Place the specimen in an esky with ice cubes and dispatch immediately. |
| Method | Call taxi from the lab. Fill out cab voucher logbook.
Send to: Victorian neuromuscular Laboratory Service (VNLS), Alfred Pathology Service, Ground Floor, main Ward Block, Commercial Road, Melbourne VIC 3004.
Contact: 9076 3150
| Send to: Victorian Neuromuscular Laboratory Service (VNLS), Alfred Pathology Service, Ground Floor, Main Ward Block, Commercial Road, Melbourne VIC 3004. Contact: 9076 3150
|
Recent changes for Mycobacterium ulcerans PCR
Recent changes for Mycobacterium ulcerans PCR |
Date | Field | Changed From | Changed To |
23rd April 2019 | Preferred Container Type | Sterile Jar Swab - white top, plain swab, plastic shaft | Sterile Jar Swab - white top, plain swab, plastic shaft Swab - blue top (Amies transport media) |
Recent changes for Plasminogen
Recent changes for Plasminogen |
Date | Field | Changed From | Changed To |
21st December 2020 | Volume (Adults) | 4 mL | 3.5 mL |
17th December 2020 | External Laboratory | Austin Pathology | Monash Pathology |
Recent changes for Platelet Function Analysis
Recent changes for Platelet Function Analysis |
Date | Field | Changed From | Changed To |
22nd August 2019 | Volume (Adults) | | 3.5 mL |
| Minimum/Paediatric Volume | | 3.5 mL |
| Collection & Request Instructions | PLEASE CONTACT THE LABORATORY BEFORE COLLECTING SPECIMENS FOR THIS TEST. Samples must be analysed within 3-4 hours of collection.
Do not use 2 mL citrate tubes.
Transport to laboratory immediately. DO NOT transport in chute.
Non - BHH Collection: Must be transported at room temperature to the Box Hill Hospital laboratory within 2 hours. Notify laboratory before collection and record medication (including all painkillers.) | PLEASE CONTACT THE LABORATORY BEFORE COLLECTING SPECIMENS FOR THIS TEST. Samples must be analysed within 3-4 hours of collection.
Do not use 2 mL citrate tubes. Required 2x citrate tubes.
Transport to laboratory immediately. DO NOT transport in chute.
Non - BHH Collection: Must be transported at room temperature to the Box Hill Hospital laboratory within 2 hours. Notify laboratory before collection and record medication (including all painkillers.) |
| Processing Instructions | DO NOT centrifuge . | DO NOT centrifuge . |
| Notes |
- Used in screening for von Willebrand's disease and aspirin therapy.
| Used in screening for von Willebrand's disease and aspirin therapy. |
Recent changes for Protein C
Recent changes for Protein C |
Date | Field | Changed From | Changed To |
19th January 2021 | Collection & Request Instructions | Transport specimen at Room Temperature to lab | |
| Collection & Request Instructions | Fill to label marker and mix by inversion.
Do not use 1 mL citrate tubes.
1 x 3.5 ml citrate tubes sufficient for Protein C, Protein S and Antithrombin 3. | Fill to label marker and mix by inversion.
Do not use 1 mL citrate tubes.
Samples must be transported to laboratory immediately. ( Within a maximun of 4 hours from collection time)
1 x 3.5 ml citrate tubes sufficient for Protein C, Protein S and Antithrombin 3. |
10th December 2020 | Volume (Adults) | | 3.5 mL |
10th December 2020 | Collection & Request Instructions | Fill to label marker and mix by inversion.
Do not use 1 mL citrate tubes.
2 x 3.5 ml citrate tubes sufficient for Protein C, Protein S and Antithrombin 3. | Fill to label marker and mix by inversion.
Do not use 1 mL citrate tubes.
1 x 3.5 ml citrate tubes sufficient for Protein C, Protein S and Antithrombin 3. |
| Notes | Results not valid if patient receiving warfarin therapy or with acute thrombotic event.
Warfarin falsely lowers Protein C levels.
Fill to label marker and mix by inversion.
Do not use 1 mL citrate tubes.
2 x 3.5 ml citrate sufficient for Protein C, Protein S and Antithrombin 3. | Results not valid if patient receiving warfarin therapy or with acute thrombotic event.
Warfarin falsely lowers Protein C levels.
Fill to label marker and mix by inversion.
Do not use 1 mL citrate tubes.
1 x 3.5 ml citrate sufficient for Protein C, Protein S and Antithrombin 3. |
Recent changes for Protein S
Recent changes for Protein S |
Date | Field | Changed From | Changed To |
19th January 2021 | Collection & Request Instructions | Fill to label marker and mix by inversion
Do not use 1 ml citrate tubes.
1 x 3.5 mL citrate tubes sufficient for Protein C, Protein S and Antithrombin 3. | Fill to label marker and mix by inversion
Do not use 1 ml citrate tubes.
Samples must be transported to laboratory immediately. ( Within a maximun of 4 hours from collection time)
1 x 3.5 mL citrate tubes sufficient for Protein C, Protein S and Antithrombin 3. |
10th December 2020 | Volume (Adults) | 7 mL | 3.5 mL |
10th December 2020 | Collection & Request Instructions | Fill to label marker and mix by inversion
Do not use 1 ml citrate tubes.
2 x 3.5 mL citrate tubes sufficient for Protein C, Protein S and Antithrombin 3. | Fill to label marker and mix by inversion
Do not use 1 ml citrate tubes.
1 x 3.5 mL citrate tubes sufficient for Protein C, Protein S and Antithrombin 3. |
| Notes | Results not valid if patient receiving warfarin therapy or with acute thrombotic event
Warfarin falsely lowers Protein S level.
Fill to label marker and mix by inversion
Do not use 1 ml citrate tubes.
2 x 3.5 mL citrate tubes sufficient for Protein C, Protein S and Antithrombin 3. | Results not valid if patient receiving warfarin therapy or with acute thrombotic event
Warfarin falsely lowers Protein S level.
Fill to label marker and mix by inversion
Do not use 1 ml citrate tubes.
1 x 3.5 mL citrate tubes sufficient for Protein C, Protein S and Antithrombin 3. |
Recent changes for Pseudocholinesterase
Recent changes for Pseudocholinesterase |
Date | Field | Changed From | Changed To |
7th February 2020 | Assay name | Cholinesterase | Pseudocholinesterase |
| Collection & Request Instructions | Please request serum BCHE activity with the Genetic test.
Genetic testing sample : EDTA whole blood 5mL minimum
| Please request serum BCHE activity with the Genetic test.
Genetic testing sample : EDTA whole blood 5mL minimum and serum for BCHE activity.
|
30th January 2020 | Alternative names | Pseudocholinesterase | Pseudocholinesterase, Butyrylcholinesterase |
| Preferred Container Type | Pink EDTA (6mL) | Pink EDTA (6mL) Serum tube with gel |
| Collection & Request Instructions | Genetic testing sample : EDTA whole blood 5mL minimum
| Please request serum BCHE activity with the Genetic test.
Genetic testing sample : EDTA whole blood 5mL minimum
|
| Processing Instructions | Genetic testing on unspun whole blood is performed by:
Molecular Genetics Department, Pathwest
QE II Medical centre,
Hospital Ave, Nedlands WA. 6009
Phone Lab Results 08 6383 4223 Spec Recepn. 08 6383 4956
Enzyme testing (serum) : RPA (Sydney) Samples can be frozen, Batched & sent weekly | Genetic testing and enzyme testing: please send samples together packed with ice pack.
Please send patient financial consent form. Cost of the test: $150. |
| External Laboratory | Pathwest Lab Med WA | Sydney South West Pathology Service(RPA) |
14th February 2019 | Request Group | | PCHOLS |
Recent changes for Quantiferon
Recent changes for Quantiferon |
Date | Field | Changed From | Changed To |
17th July 2019 | Collection & Request Instructions | PLEASE NOTE : Quantiferon specimens must now be collected using a dedicated Quantiferon Collection Pack containing four tubes (see image above)
Specimen must be tested within 10 hours of collection.
DO NOT refrigerate specimen.
Collect Monday - Friday before 10:00am.
Used to detect latent infection with M.tuberculosis, not for the diagnosis and management of active tuberculosis.
Note: Heparinised blood required, minimum 5 mL
| PLEASE NOTE : Quantiferon specimens must now be collected using a dedicated Quantiferon Collection Pack containing four tubes (see image above)
Specimen must be received at Box Hill by 16:00hrs Monday-Friday
DO NOT refrigerate specimen.
Used to detect latent infection with M.tuberculosis, not for the diagnosis and management of active tuberculosis.
Collection Centre
|
Latest Collection time
|
Latest courier time for transport
to BHH Microbiology
(must be received by 1600hrs)
|
Box Hill
|
1400 hrs
|
N/A
|
Maroondah
|
1400 hrs
|
1450 hrs
|
Angliss
|
1400 hrs
|
1415 hrs
|
Wantirna Health
|
1400 hrs
|
1515 hrs
|
Yarra Ranges
|
1100 hrs
|
1115 hrs
|
Peter James Centre
|
1100 hrs
|
1205 hrs
1020 hrs
|
Healesville/Yarra Valley
|
1200 hrs
|
1240 hrs
|
|
| Processing Instructions | If the specimen is collected at Maroondah or Angliss Hospitals, please contact the BH Microbiology Dept (9895 3475) before sending the samples.
Do not centrifuge, refrigerate or freeze specimen. | If the specimen is collected outside of Box Hill Hospital, please contact the BH Microbiology Dept (9895 3475) before sending the samples.
Do not centrifuge, refrigerate or freeze specimen. |
| External Transport Instructions | When packing for transport, the specimens MUST be packed in a separate transport esky at room temperature.
DO NOT put in an esky with cold packs.
FOR MICROBIOLOGY STAFF
QUANTIFERON GOLD PROCESSING
Quantiferon Gold specimens must be processed as below before sending to VIDRL
Note: Tubes must be incubated within 16 hours of collection.
At 12.00-12.30 mix the Quantiferon Gold tubes by inverting 10 times.
Incubate the tubes UPRIGHT in incubator M120.
Write time of incubation in F8 notes.
The next day at 8 am centrifuge the tubes for 15 mins at 3000g
(Tubes must be incubated for 16 to 24 hours.)
Check tubes for complete separation, respin if necessary.
The tubes are then ready to be packed and sent to VIDRL
Write the incubation time (from F8 notes) on the cover sheet.
NOTE : There is no change to collection times. Store & transport specimens at room temperature. | When packing for transport, the specimens MUST be packed in a separate transport esky at room temperature.
DO NOT put in an esky with cold packs.
FOR MICROBIOLOGY STAFF
Quantiferon Gold specimens must be processed as below before sending to VIDRL
Note:
Tubes must be incubated within 16 hours of collection (keep at room temperature until incubation);
Incubation at 37°C must be for 16-24 hours;
After incubation, tubes must be centrifuged within 3 days (keep at room temperature until centrifugation).
QUANTIFERON GOLD PROCESSING
Processing: to be performed by Microbiology staff
Remix all tubes immediately before incubation by inverting 10 times
Incubate at 37°C, O2 (M138 incubator). There is a metal rack in the incubator to stand the tubes upright during incubation
For specimens received between 0800 hrs and 1600 hrs, incubate overnight.
For specimens received after 1600 hrs, hold at room temperature and incubate at 0800 hrs the next day (for 24 hours) and sent to VIDRL on the following day (i.e. two days later)
At 0800hr each day, remove specimens from incubator and centrifuge for 15 minutes at 3000 RCF (g). The laboratory assistant will do this on weekdays, Blood Culture Bench Scientist will do this on Saturday and Sunday
After centrifugation, send specimens to VIDRL with the 1230 hrs courier
Specimens that are received in the laboratory, greater than 10 hours after collection will not be tested (KIMMS). |
Recent changes for RAST
Recent changes for RAST |
Date | Field | Changed From | Changed To |
1st April 2019 | Collection & Request Instructions | White top tube is the preferred sample.
0.2ml of blood required for each allergen.
Blood collection: still can be done after skin allergy testing. | White top tube is the preferred sample.0.2ml of blood required for each allergen.
Blood collection: still can be done after skin allergy testing.
Omega-5-Gliadin: serum send to Melbourne Pathology
IgG 96 Food Allergy test is not a RAST test It is performed at Australian Clinical Labs.This test is NOT rebatable and costs approx $260. |
| Processing Instructions | IgG 96 Food Allergy test is not a RAST test It is performed at Australian Clinical Labs.This test is NOT rebatable and costs approx $260.
| Omega-5-Gliadin: send to Melborne Pathology
IgG 96 Food Allergy test is not a RAST test It is performed at Australian Clinical Labs.This test is NOT rebatable and costs approx $260.
|
Recent changes for Renin - plasma
Recent changes for Renin - plasma |
Date | Field | Changed From | Changed To |
3rd December 2019 | Collection & Request Instructions |
Collection Requirements for both or either tests
Collect into 4 mL EDTA tubes at Room Temperature. Do not collect on ice
If both Renin and Aldosterone are requested together, they can be collected into the same EDTA tube
In the laboratory, spin and separate the plasma immediately. Plasma is stable at Room Temperature if analysis is performed on the same day.
Renin must be frozen at -80 C. Transport plasma at room temperature until it can be place in -80 C Freezer.(Must be done within 3-4).
Add-on tests for Renin and Aldosterone should not be done on samples which have been previously collected or stored at 4 C.
Renin will not be collected at Healesville Hospital and Yarra Ranges Health Collection Rooms. |
Collection Requirements for both or either tests
Collect into 4 mL EDTA tubes at Room Temperature. Do not collect on ice
If both Renin and Aldosterone are requested together, they can be collected into the same EDTA tube
In the laboratory, spin and separate the plasma immediately. Plasma is stable at Room Temperature if analysis is performed on the same day.
Renin must be frozen at -80 C. Transport plasma at room temperature (Maroondah and Angliss) until it can be place in -80 C Freezer.(Must be done within 3-4 hors).
Add-on tests for Renin and Aldosterone should not be done on samples which have been previously collected or stored at 4 C.
Renin will not be collected at Healesville Hospital and Yarra Ranges Health Collection Rooms. |
3rd December 2019 | Collection & Request Instructions |
Collection Requirements for both or either tests
-
Collect into 4 mL EDTA tubes at Room Temperature. Do not collect on ice
-
If both Renin and Aldosterone are requested together, they can be collected into the same EDTA tube
-
In the laboratory, spin and separate the plasma immediately. Plasma is stable at Room Temperature if analysis is performed on the same day
-
If the specimen arrives on the weekend or after hours, or if the specimen is referred from an external laboratory, freeze the plasma
-
EDTA plasma samples from external laboratories should be stored and transported frozen
-
Whole blood or plasma stored at 4 Deg C is not suitable
-
Add-on tests for Renin and Aldosterone should not be done on samples which have been previously collected or stored at 4 Deg C
|
Collection Requirements for both or either tests
Collect into 4 mL EDTA tubes at Room Temperature. Do not collect on ice
If both Renin and Aldosterone are requested together, they can be collected into the same EDTA tube
In the laboratory, spin and separate the plasma immediately. Plasma is stable at Room Temperature if analysis is performed on the same day.
Renin must be frozen at -80 C. Transport plasma at room temperature until it can be place in -80 C Freezer.(Must be done within 3-4).
Add-on tests for Renin and Aldosterone should not be done on samples which have been previously collected or stored at 4 C.
Renin will not be collected at Healesville Hospital and Yarra Ranges Health Collection Rooms. |
| Processing Instructions |
Spin,seperate and freeze, transport frozen.
Minimum plasma volume: 500 ul (1mL if aldosterone is also requested) | Spin,seperate and freeze (-80 C), transport frozen.
Minimum plasma volume: 500 ul (1mL if aldosterone is also requested) |
| Notes |
Prior to testing, patients should be withdrawn from diuretics, ACE inhibitors, angiotensin II receptor antagonists, beta blockers and calcium channel blockers for 3 weeks. Patients should also be well hydrated, have had 7g/day of salt for one week and have normal plasma potassium and bicarbonate levels.
-
Deliver to laboratory ASAP at room temperature
| Prior to testing, patients should be withdrawn from diuretics, ACE inhibitors, angiotensin II receptor antagonists, beta blockers and calcium channel blockers for 3 weeks. Patients should also be well hydrated, have had 7g/day of salt for one week and have normal plasma potassium and bicarbonate levels.
Deliver to laboratory ASAP at room temperature |
Recent changes for Respiratory Virus PCR
Recent changes for Respiratory Virus PCR |
Date | Field | Changed From | Changed To |
3rd July 2019 | Notes | This PCR will detect and identify: Influenza A virus, Influenza B virus and Respiratory syncytial virus (RSV).
Transport at room temperature to the Laboratory
Testing is performed at Box Hill, Angliss, and Maroondah.
Testing Lab
|
Testing hours
|
TAT (from receipt in lab)
|
All sites
|
Available 24/7
|
1 hr
|
Samples received after hours may have a longer TAT due to other urgent critical pathology testing occuring. | This PCR will detect and identify: Influenza A virus, Influenza B virus and Respiratory syncytial virus (RSV).
Transport at room temperature to the Laboratory
Testing is performed at Box Hill, Angliss, and Maroondah.
Testing Lab
|
Testing hours
|
TAT (from receipt in lab)
|
All sites
|
Available 24/7
|
1 hr
|
Samples received after hours may have a longer TAT due to other urgent critical pathology testing occurring. |
3rd July 2019 | Notes | This PCR will detect and identify: Influenza A virus, Influenza B virus and Respiratory syncytial virus (RSV).
Transport at room temperature to the Laboratory
Testing is performed at Box Hill, Angliss, and Maroondah.
Testing Lab
|
Testing hours
|
TAT (from receipt in lab)
|
All sites
|
Available 24/7
|
1 hr
|
Samples received after hours may have a longer TAT due to other urgent critical pathology testing occuring. | This PCR will detect and identify: Influenza A virus, Influenza B virus and Respiratory syncytial virus (RSV).
Transport at room temperature to the Laboratory
Testing is performed at Box Hill, Angliss, and Maroondah.
Testing Lab
|
Testing hours
|
TAT (from receipt in lab)
|
All sites
|
Available 24/7
|
1 hr
|
Samples received after hours may have a longer TAT due to other urgent critical pathology testing occuring. |
3rd July 2019 | Notes | This PCR will detect and identify: Influenza A virus, Influenza B virus and Respiratory syncytial virus (RSV).
Transport at room temperature to the Laboratory
Testing is performed at Box Hill, Angliss, and Maroondah.
Testing Lab
|
Testing hours
|
TAT (from receipt in lab)
|
All sites
|
Available 24/7
|
1 hr
|
TAT is a guide only. Minimal staffing levels after hours may mean a longer TAT due to other urgent pathology testing occuring (ie urgent Troponin, urgent transfusions etc). | This PCR will detect and identify: Influenza A virus, Influenza B virus and Respiratory syncytial virus (RSV).
Transport at room temperature to the Laboratory
Testing is performed at Box Hill, Angliss, and Maroondah.
Testing Lab
|
Testing hours
|
TAT (from receipt in lab)
|
All sites
|
Available 24/7
|
1 hr
|
Samples received after hours may have a longer TAT due to other urgent critical pathology testing occuring. |
3rd July 2019 | Method | All tests for respiratory virus PCR are considered urgent and must be delivered to the laboratory ASAP. Overnight specimens collected at BHH must be delivered to Microbiology by 7 a.m. Monday-Sunday.
If ICU/Oncology patients tested Flu A/B and RSV negative on GeneXpert will be referred to Monash for respiratory virus panel. This includes Influenza A virus, Influenza B virus, Respiratory Syncitial Virus, Picornavirus (RV/EV), Parainfluenza viruses 1,2 and 3, Adenovirus, Human Metapneumovirus, Bordetella pertussis, Bordetella parapertussis, Mycoplasma pneumoniae, Legionella species, Chlamydiaceae.
The following specimens will be referred for testing-
Sputum, Bronchial Washings, Bronchoalveolar Lavages
Specimens for Respiratory Multiplex PCR will be referred to Monash Molecular Laboratory
Contact Monash Pathology 9594 4532 or if no answer try Bacteriology 9594 4565 as there is someone rostered 24/7
| All tests for respiratory virus PCR are considered urgent and must be delivered to the laboratory ASAP.
ICU/Oncology patients testing negative for Flu A/B and RSV will be referred to Monash for respiratory virus panel. This includes Influenza A virus, Influenza B virus, Respiratory Syncitial Virus, Picornavirus (RV/EV), Parainfluenza viruses 1,2 and 3, Adenovirus, Human Metapneumovirus, Bordetella pertussis, Bordetella parapertussis, Mycoplasma pneumoniae, Legionella species, Chlamydiaceae.
The following specimens will be referred for testing-
Sputum, Bronchial Washings, Bronchoalveolar Lavages
Specimens for Respiratory Multiplex PCR will be referred to Monash Molecular Laboratory
Contact Monash Pathology 9594 4532 or if no answer try Bacteriology 9594 4565 as there is someone rostered 24/7
|
| Notes | This PCR will detect and identify: Influenza A virus, Influenza B virus and Respiratory syncytial virus (RSV).
Transport at room temperature to the Laboratory
Testing is performed at BHH and MH.
Testing Lab
|
Testing hours
|
TAT (from receipt in lab)
|
BHH
– all sites except MH
|
Mon-Fri 0700-2000 hrs
Sat & Sun 0700-1630 hrs
|
1 hr
|
MH
|
1 June – 31 August 2018 24/7
Other times as for BHH
|
I hr
|
| This PCR will detect and identify: Influenza A virus, Influenza B virus and Respiratory syncytial virus (RSV).
Transport at room temperature to the Laboratory
Testing is performed at Box Hill, Angliss, and Maroondah.
Testing Lab
|
Testing hours
|
TAT (from receipt in lab)
|
All sites
|
Available 24/7
|
1 hr
|
TAT is a guide only. Minimal staffing levels after hours may mean a longer TAT due to other urgent pathology testing occuring (ie urgent Troponin, urgent transfusions etc). |
Recent changes for Risperidone
Recent changes for Risperidone |
Date | Field | Changed From | Changed To |
18th December 2020 | External Laboratory | Pathwest Lab Med WA | Alfred Hospital |
Recent changes for Test
Recent changes for Test |
Date | Field | Changed From | Changed To |
3rd December 2020 | Method | Laboratory notes testing | Laboratory notes - email test |
Recent changes for Testosterone
Recent changes for Testosterone |
Date | Field | Changed From | Changed To |
15th February 2019 | Processing Instructions | | Free Testosterone/Calculated free testosterone requested: test code CFTEST
Also add Testosterone/SHBG/Albumin if free testosterone requested. |
Recent changes for Thiopurine Metabolites
Recent changes for Thiopurine Metabolites |
Date | Field | Changed From | Changed To |
3rd September 2019 | Collection & Request Instructions |
Collect 2x4ml EDTA blood. DO NOT SPIN
Keep at 4 deg C, do not freeze.
The metabolites are stable for upto 4 days at 4 degress. | Collect 2x4ml EDTA blood. DO NOT SPIN
Keep at 4 deg C, do not freeze.
The metabolites are stable for upto 4 days at 4 degress. |
| Notes |
Imuran is a trade name for azathioprine
The Thiopurine metabolites (6-TGN & 6-MMP) are used as a means of estimating the toxicity and efficacy of azathioprine treatment.
This test is performed by the Gastroenerology department. ( Phone No.9895 4632 )
The cost for the test is not covered by Medicare. Contact the testing lab for cost details. | Imuran is a trade name for azathioprine
The Thiopurine metabolites (6-TGN & 6-MMP) are used as a means of estimating the toxicity and efficacy of azathioprine treatment.
This test is performed by the Gastroenerology department. ( Phone No.9895 4632 )
The cost for the test is not covered by Medicare.
Eastern Health patients:6TGN 6MMP Thiopurine Metabolites Testing $75
All other patients: 6TGN 6MMP Thiopurine Metabolites Testing $145 |
Recent changes for Thrombophilia Screen
Recent changes for Thrombophilia Screen |
Date | Field | Changed From | Changed To |
10th December 2020 | Collection & Request Instructions | For full thrombophilia screen including INR, APTT, Protein C, Protein S, AT3, LA screen, APCR, Homocystine, Factor V Leiden, PG20210A and ACA:-
5 x 3.5 ml citrate tubes plus 2 EDTA tubes and 1 x serum tube will be required. | For full thrombophilia screen including INR, APTT, Protein C, Protein S, AT3, LA screen, , Homocystine, Factor V Leiden, PG20210A and ACA/ACL antibodies:-
3 x 3.5 ml citrate tubes plus 2 EDTA tubes and 1 x serum tube will be required. |
| Notes | May include Protein C functional, Protein S free, Antithrombin III, APCR ratio, Factor V Leiden, Homocystine, Anti-cardiolipin antibodies, Lupus Anticoagulant, Prothrombin mutation.
Which tests are most appropriate depends on clinical history
Tests need to be listed individually to comply with HIC guidelines
Consult Haematology Registrar or Haematologist.
For full thrombophilia screen including INR, APTT, Protein C, Protein S, AT3, LA screen, APCR, Homocystine, Factor V Leiden, PG20210A and ACA:- 5 x 3.5 ml citrate tubes plus 2 EDTA tubes and 1 x serum tube will be required. | May include Protein C functional, Protein S free, Antithrombin III, Factor V Leiden, Homocystine, Anti-cardiolipin antibodies, Lupus Anticoagulant, Prothrombin Gene Mutation.
Which tests are most appropriate depends on clinical history
Tests need to be listed individually to comply with HIC guidelines
Consult Haematology Registrar or Haematologist.
For full thrombophilia screen including INR, APTT, Protein C, Protein S, AT3, LA screen, Homocystine, Factor V Leiden, PG20210A and ACA:- 3 x 3.5 ml citrate tubes plus 2 EDTA tubes and 1 x serum tube will be required. |
Recent changes for Urine Nickel
Recent changes for Urine Nickel |
Date | Field | Changed From | Changed To |
17th January 2020 | External Laboratory | Sydney South West Pathology Service(RPA) | Alfred Hospital |
| Frequency | | As required |
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