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Recent Changes - Test & Collection Guide

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 Recent changes for Adrenocorticotrophic Hormone

Recent changes for Adrenocorticotrophic Hormone

Date
Field
Changed From
Changed To
2nd August 2022
Notes

Consultation with Endocrinologist suggested

Collect in the morning between 0800 and 1000h.

1 x 4mL EDTA tube on ice.

Send to laboratory for immediate centrifugation. Spin, separate and freeze plasma. Transport frozen.

 

 

 

Consultation with Endocrinologist suggested

Collect 1 x 4mL EDTA tube on ice.

Send to laboratory for immediate centrifugation. Spin, separate and freeze plasma. 

 

 

 


 Recent changes for Anti-diuretic hormone

Recent changes for Anti-diuretic hormone

Date
Field
Changed From
Changed To
7th October 2021
Alternative names

ADH

Request Group

ADH

Preferred Specimen Type

Plasma

Serum

Optional Specimen Type

Plasma

Preferred Container Type

EDTA

Serum tube with gel

Optional Container Type

EDTA
Lithium heparin no gel

Collection & Request Instructions

Samples should be fasting.

Processing Instructions

Spin, separate and freeze plasma.

Packed by certified personnel.

Sent frozen.

Specimen Reception, Level 6 (E Block), RPA, Missenden Road, Camperdown , NSW 2050

Tel: 02-95158279

Spin, separate and freeze serum/plasma

Packed by certified personnel.

Sent frozen.

Specimen Reception, Level 6 (E Block), RPA, Missenden Road, Camperdown , NSW 2050

Tel: 02-95158279


 Recent changes for Anti-Thrombin III

Recent changes for Anti-Thrombin III

Date
Field
Changed From
Changed To
19th January 2021
Collection & Request Instructions

Fill to label marker and mix by inversion.

DO NOT use 1 mL citrate tubes.

1 x 3.5 ml citrate tubes sufficient for Protein C, Protein S and Antithrombin 3.

Fill to label marker and mix by inversion.

DO NOT use 1 mL citrate tubes.

Samples must be transported to laboratory immediately. ( Within a maximun of 4 hours from collection time)

1 x 3.5 ml citrate tubes sufficient for Protein C, Protein S and Antithrombin 3.

10th December 2020
Collection & Request Instructions

Fill to label marker and mix by inversion.

DO NOT use 1 mL citrate tubes.

Fill to label marker and mix by inversion.

DO NOT use 1 mL citrate tubes.

1 x 3.5 ml citrate tubes sufficient for Protein C, Protein S and Antithrombin 3.


 Recent changes for Anti-Xa Assay

Recent changes for Anti-Xa Assay

Date
Field
Changed From
Changed To
18th May 2021
Request Group

10A: Clexane, 10AH:Plasma Heparin, APIX: Apixaban, RIVX: Rivaroxaban

10A: Clexane, 10AH:Plasma Heparin, APIX: Apixaban, RIVX: Rivaroxaban, FONX: Fondaparinux

18th May 2021
Request Group

10A

10A: Clexane, 10AH:Plasma Heparin, APIX: Apixaban, RIVX: Rivaroxaban


 Recent changes for Bile Acids

Recent changes for Bile Acids

Date
Field
Changed From
Changed To
23rd May 2022
Laboratory

Referred Test

Biochemistry

Preferred Container Type

Plain Tube with gel

Serum tube with gel

Collection & Request Instructions

Fasting or Non Fasting sample.

Note: This test is ONLY performed on pregnant women.

 

Fasting.

Note: This test is ONLY performed on pregnant women.

 

Processing Instructions

Spin, separate ASAP.

External Laboratory

Royal Childrens Hospital

Frequency

Weekly

Twice weekly

Method

Store and transport at 4 C.


 Recent changes for Blood Cultures - Adult

Recent changes for Blood Cultures - Adult

Date
Field
Changed From
Changed To
9th August 2021
Processing Instructions

Bottles should be loaded on to the Bactec analyser as soon as possible. If entry is delayed, store bottles at room temperature.

Bottles should be loaded on to the Bactec FX analyser as soon as possible. If entry is delayed, store bottles at room temperature.

9th August 2021
Processing Instructions

Bottles should be loaded on to the Virtuo analyser as soon as possible. If entry is delayed, store bottles at room temperature.

Bottles should be loaded on to the Bactec analyser as soon as possible. If entry is delayed, store bottles at room temperature.

25th May 2021
Volume (Adults)

10 mL

8 mL

Preferred Container Type

Blood Culture

Blood Culture (Adult)


 Recent changes for Blood Cultures - Children and Neonates

Recent changes for Blood Cultures - Children and Neonates

Date
Field
Changed From
Changed To
9th August 2021
External Transport Instructions

Load as soon as possible onto the Virtuo Analyzer. Store at room temperature until loaded

Load as soon as possible onto the Bactec FX Analyzer. Store at room temperature until loaded

25th May 2021
Volume (Adults)

4 mL

Minimum/Paediatric Volume

1 mL

Preferred Container Type

Blood Culture Paediatric

Blood Culture (Paediatric)

Collection & Request Instructions

Add 0.5 - 4mL of blood to a single aerobic paediatric bottle.

For patients with suspected bacterial endocarditis, 2 to 3 sets taken at different times should be sufficient to establish a diagnosis.

Collect blood cultures 20 minutes apart. If patient is too ill to wait 20 minutes, prior to administering antibiotics, collect the first set from one site and the second from a different site.

Please provide clinical details. Extended incubation is required for query endocarditis, brucellosis or fungal infection

Transport at room temperature

 

Add 1 - 3mL of blood to a single aerobic paediatric bottle.

For patients with suspected bacterial endocarditis, 2 to 3 sets taken at different times should be sufficient to establish a diagnosis.

If multiple sets required, collect blood cultures 20 minutes apart. If patient is too ill to wait 20 minutes, prior to administering antibiotics, collect the first set from one site and the second from a different site.

Please provide clinical details. Extended incubation is required for query endocarditis, brucellosis or fungal infection

Transport at room temperature

 


 Recent changes for Bone Marrow Examination

Recent changes for Bone Marrow Examination

Date
Field
Changed From
Changed To
25th March 2022
Laboratory

Unassigned

Haematology


 Recent changes for Bone Specific Alkaline Phosphatase

Recent changes for Bone Specific Alkaline Phosphatase

Date
Field
Changed From
Changed To
15th June 2021
Assay name

Bone Specific Alkiline Phosphatase

Bone Specific Alkaline Phosphatase

External Laboratory

Monash Pathology

Royal Melbourne Hospital


 Recent changes for BRCA1/BRCA2

Recent changes for BRCA1/BRCA2

Date
Field
Changed From
Changed To
17th June 2021
Request Group

CHROM

Collection & Request Instructions

Send to Peter Mac on next available courier. Stable at room temperature for a maximum of 2 weeks.

Processing Instructions

Send to Peter Mac on next available courier. Stable at room temperature for a maximum of 2 weeks.

External Transport Instructions

Room Temperature

Room Temperature

Send to Peter Mac on next available courier. Stable at room temperature for a maximum of 2 weeks.

Method

Send to Peter Mac on next available courier. Stable at room temperature for a maximum of 2 weeks.


 Recent changes for CASA

Recent changes for CASA

Date
Field
Changed From
Changed To
17th February 2022
External Laboratory

Royal Brisbane & Womens Hospital

External Transport Instructions

  • Transport frozen.
  • Packed by certified personnel.
  • Transport frozen.

    Packed by certified personnel.

    Notes

    In combination with the tumour marker CA-125, CASA is used for differentiating between benign gynaecological disease and ovarian carcinoma.  Please note that this test has changed referral laboratories as at 18/1/2011.

    Please contact Chemical Pathologist before requesting this test.

    In combination with the tumour marker CA-125, CASA is used for differentiating between benign gynaecological disease and ovarian carcinoma.  Please note that this test has changed referral laboratories as at 18/1/2011.  This test is no longer available in Australia

    Please contact Chemical Pathologist before requesting this test.

     


     Recent changes for Cerebrospinal Fluid - Biochemistry

    Recent changes for Cerebrospinal Fluid - Biochemistry

    Date
    Field
    Changed From
    Changed To
    27th April 2021
    Collection & Request Instructions

    Please note : to avoid sample deterioration, DO NOT send CSF specimens via the vacuum chute system.

    Depending on the tests requested, 3-4 separate tubes will need to be provided.  Note that the tubes are numbered so that order of collection can be identified – this order should be carefully followed, so that the optimal sample can be used for the requested tests.  It is also important to consider the tests that are required, as this will determine how much CSF is needed in each tube.  See the matrix below for details.

    TUBE 1 is used for Cytology.

    TUBE 2 is used for Glucose, Protein and Flow Cytometry.

    (centrifuged in the lab – the supernatant is used for Biochemistry analysis (glucose, protein) and the cellular pellet will be used for flow cytometry if required).

    TUBE 3 is for Microbiological analysis (cell count, Gram stain and culture) and any further infectious testing (e.g. viral PCR, mycobacterial PCR and culture, cryptococcal antigen).

    TUBE 4 will be required only for;

             (a) Creutzfeld-Jacob Disease (CJD) testing OR

    (b) Xanthochromia analysis (wrap the tube in foil to prevent    sample deterioration due to light).

     

    TESTS REQUIRED

    MINIMUM REQUIRED VOLUME (mL)

    TUBE 1

    TUBE 2

    TUBE 3

    TUBE 4

    CYTOLOGY. CHEMISTRY, MICRO/CULTURE

    1.0-2.0

    1.0

    1.0-2.0

    Nil

    ADD FLOW CYTOMETRY

     

    add 5.0-7.0

     

     

    ADD OTHER INFECTIOUS MARKERS

     

     

    add 2.0- 5.0-

     

    ADD MYCOBACTERIAL CULTURE

     

     

    Add 10.00

     

    ADD CJD

     

     

     

    1.0

    ADD XANTHOCHROMIA

     

     

     

    1.0 (foil)

     

    Note, the ability to add further tests to a previously collected sample is dependent upon the volume provided.  Also, many tests (flow cytometry, cytology) can only be performed on fresh specimens.

     

    Other specialised testing (e.g. oligoclonal bands) will be performed using the supplied samples.

     

    Xanthochromia and CJD analysis cannot be performed on a blood-stained sample.

    CSF Information - Laboratory Processing

    Depending on the tests requested, 3-4 separate tubes will need to be provided. Note that the tubes are numbered so that order of collection can be identified – this order should be carefully followed, so that the optimal sample can be used for the requested tests. It is also important to consider the tests that are required, as this will determine how much CSF is needed in each tube. See the matrix below for details.

    TUBE 1 is used for Biochemistry and Flow Cytometry. (Centrifuged in the lab – the supernatant is used for Biochemistry analysis (glucose, protein) and the cellular pellet will be used for flow cytometry if required). If tube 3 is bloodstained, take an aliquot for microbiology.

    TUBE 2 is used for Cytology

    TUBE 3 is for Microbiological analysis (cell count, Gram stain and culture) and any further infectious testing (e.g. viral PCR, mycobacterial PCR and culture, cryptococcal antigen).

    TUBE 4 will be required only for;

    (a) Creutzfeld-Jacob Disease (CJD) testing OR

    (b) Xanthochromia analysis (wrap the tube in foil to prevent sample deterioration due to light).

     

    TESTS REQUIRED

    MINIMUM REQUIRED VOLUME (mL)

    TUBE 1

    TUBE 2

    TUBE 3

    TUBE 4

    CHEMISTRY

    1.0

         

    ADD FLOW CYTOMETRY

    Minimum 5.0-7.0

         

    CYTOLOGY

     

    1.0-3.0

       

    MICROBIOLOGY

       

    1.0-2.0

     

    ADD OTHER INFECTIOUS MARKERS

       

    add 2.0- 5.0-

     

    ADD MYCOBACTERIAL CULTURE

       

    Add 6.0-10.00

     

    ADD CJD

         

    1.0

    ADD XANTHOCHROMIA

         

    1.0 (foil)

     

    Note, the ability to add further tests to a previously collected sample is dependent upon the volume provided. Also, many tests (flow cytometry, cytology) can only be performed on fresh specimens.

     

    Other specialised testing (e.g. oligoclonal bands) will be performed using the supplied samples.

    Xanthochromia and CJD analysis cannot be performed on a blood-stained sample.

     


     Recent changes for Cerebrospinal Fluid - Cytology

    Recent changes for Cerebrospinal Fluid - Cytology

    Date
    Field
    Changed From
    Changed To
    27th April 2021
    Collection & Request Instructions

    The sample with the least amount of blood is prefered for Cytology.

    CSF SPECIMEN INFORMATION – LABORATORY PROCESSING

    Depending on the tests requested, 3-4 separate tubes will need to be provided.  Note that the tubes are numbered so that order of collection can be identified – this order should be carefully followed, so that the optimal sample can be used for the requested tests.  It is also important to consider the tests that are required, as this will determine how much CSF is needed in each tube.  See the matrix below for details.

    TUBE 1 is used for Cytology.

    TUBE 2 is used for Glucose, Protein and Flow Cytometry.

    (centrifuged in the lab – the supernatant is used for Biochemistry analysis (glucose, protein) and the cellular pellet will be used for flow cytometry if required).

    TUBE 3 is for Microbiological analysis (cell count, Gram stain and culture) and any further infectious testing (e.g. viral PCR, mycobacterial PCR and culture, cryptococcal antigen).

    TUBE 4 will be required only for;

             (a) Creutzfeld-Jacob Disease (CJD) testing OR

    (b) Xanthochromia analysis (wrap the tube in foil to prevent    sample deterioration due to light).

     

    TESTS REQUIRED

    MINIMUM REQUIRED VOLUME (mL)

    TUBE 1

    TUBE 2

    TUBE 3

    TUBE 4

    CYTOLOGY. CHEMISTRY, MICRO/CULTURE

    1.0-2.0

    1.0

    1.0-2.0

    Nil

    ADD FLOW CYTOMETRY

     

    add 5.0-7.0

     

     

    ADD OTHER INFECTIOUS MARKERS

     

     

    add 2.0- 5.0-

     

    ADD MYCOBACTERIAL CULTURE

     

     

    Add 10.00

     

    ADD CJD

     

     

     

    1.0

    ADD XANTHOCHROMIA

     

     

     

    1.0 (foil)

     

    Note, the ability to add further tests to a previously collected sample is dependent upon the volume provided.  Also, many tests (flow cytometry, cytology) can only be performed on fresh specimens.

     

    Other specialised testing (e.g. oligoclonal bands) will be performed using the supplied samples.

     

    Xanthochromia and CJD analysis cannot be performed on a blood-stained sample.

    The sample with the least amount of blood is prefered for Cytology.

    CSF SPECIMEN INFORMATION – LABORATORY PROCESSING

    Depending on the tests requested, 3-4 separate tubes will need to be provided.  Note that the tubes are numbered so that order of collection can be identified – this order should be carefully followed, so that the optimal sample can be used for the requested tests.  It is also important to consider the tests that are required, as this will determine how much CSF is needed in each tube.  See the matrix below for details.

    TUBE 1 is used for Biochemistry and Flow Cytometry. (Centrifuged in the lab – the supernatant is used for Biochemistry analysis (glucose, protein) and the cellular pellet will be used for flow cytometry if required). If tube 3 is bloodstained, take an aliquot for microbiology.

    TUBE 2 is used for Cytology

    TUBE 3 is for Microbiological analysis (cell count, Gram stain and culture) and any further infectious testing (e.g. viral PCR, mycobacterial PCR and culture, cryptococcal antigen).

    TUBE 4 will be required only for;

    (a) Creutzfeld-Jacob Disease (CJD) testing OR

    (b) Xanthochromia analysis (wrap the tube in foil to prevent sample deterioration due to light).

     

    TESTS REQUIRED

    MINIMUM REQUIRED VOLUME (mL)

    TUBE 1

    TUBE 2

    TUBE 3

    TUBE 4

    CHEMISTRY

    1.0

         

    ADD FLOW CYTOMETRY

    Minimum 5.0-7.0

         

    CYTOLOGY

     

    1.0-3.0

       

    MICROBIOLOGY

       

    1.0-2.0

     

    ADD OTHER INFECTIOUS MARKERS

       

    add 2.0- 5.0-

     

    ADD MYCOBACTERIAL CULTURE

       

    Add 6.0-10.00

     

    ADD CJD

         

    1.0

    ADD XANTHOCHROMIA

         

    1.0 (foil)

     

    Note, the ability to add further tests to a previously collected sample is dependent upon the volume provided. Also, many tests (flow cytometry, cytology) can only be performed on fresh specimens.

     

    Other specialised testing (e.g. oligoclonal bands) will be performed using the supplied samples.

    Xanthochromia and CJD analysis cannot be performed on a blood-stained sample.

     

     

    Method

    CSF sample processing:

    If Cytology/Flow Cytometry requested  : Tube 1-Biochemistry, Tube 2- Cytology/Flow Cytometry, Tube 3-Microbiology

    If Cytology/Flow Cytometry not requested: Tube 1 and 3-Microbiology, Tube 2-Biochemistry

    Any confusion between the terms cell count and cytology could be cleared up when the cell count is rung through to the requesting doctor by the scientist. At this point it could be determined whether cytology is a genuine request or not (all to be documented in F8 notes).

     

     


     Recent changes for Cerebrospinal Fluid - microscopy and culture

    Recent changes for Cerebrospinal Fluid - microscopy and culture

    Date
    Field
    Changed From
    Changed To
    27th April 2021
    Notes

    CSF SPECIMEN INFORMATION – LABORATORY PROCESSING

    Depending on the tests requested, 3-4 separate tubes will need to be provided.  Note that the tubes are numbered so that order of collection can be identified – this order should be carefully followed, so that the optimal sample can be used for the requested tests.  It is also important to consider the tests that are required, as this will determine how much CSF is needed in each tube.  See the matrix below for details.

    TUBE 1 is used for Cytology.

    TUBE 2 is used for Glucose, Protein and Flow Cytometry.

    (centrifuged in the lab – the supernatant is used for Biochemistry analysis (glucose, protein) and the cellular pellet will be used for flow cytometry if required).

    TUBE 3 is for Microbiological analysis (cell count, Gram stain and culture) and any further infectious testing (e.g. viral PCR, mycobacterial PCR and culture, cryptococcal antigen).

    TUBE 4 will be required only for;

             (a) Creutzfeld-Jacob Disease (CJD) testing OR

    (b) Xanthochromia analysis (wrap the tube in foil to prevent    sample deterioration due to light).

     

    TESTS REQUIRED

    MINIMUM REQUIRED VOLUME (mL)

    TUBE 1

    TUBE 2

    TUBE 3

    TUBE 4

    CYTOLOGY. CHEMISTRY, MICRO/CULTURE

    1.0-2.0

    1.0

    1.0-2.0

    Nil

    ADD FLOW CYTOMETRY

     

    add 5.0-7.0

     

     

    ADD OTHER INFECTIOUS MARKERS

     

     

    add 2.0- 5.0-

     

    ADD MYCOBACTERIAL CULTURE

     

     

    Add 10.00

     

    ADD CJD

     

     

     

    1.0

    ADD XANTHOCHROMIA

     

     

     

    1.0 (foil)

     

    Note, the ability to add further tests to a previously collected sample is dependent upon the volume provided.  Also, many tests (flow cytometry, cytology) can only be performed on fresh specimens.

     

    Other specialised testing (e.g. oligoclonal bands) will be performed using the supplied samples.

     

    Xanthochromia and CJD analysis cannot be performed on a blood-stained sample.

    Special tests available on request:

    PCR for viruses

    Acid-fast stain and/or culture for Mycobacteria

    Cryptococcal antigen

    Serological test for Syphilis (must be paired with 5mL serum sample or test will not be performed)

    Viral Serology, if CSF is accompanied by serum sample

    Flow Cytometry - requires a 4th tube

    The turnaround time for CSF microscopy are 30-40 minutes.

    The turnaround time for CSF culture are 3-5 days.

    Depending on the tests requested, 3-4 separate tubes will need to be provided. Note that the tubes are numbered so that order of collection can be identified – this order should be carefully followed, so that the optimal sample can be used for the requested tests. It is also important to consider the tests that are required, as this will determine how much CSF is needed in each tube. See the matrix below for details.

    TUBE 1 is used for Biochemistry and Flow Cytometry. (Centrifuged in the lab – the supernatant is used for Biochemistry analysis (glucose, protein) and the cellular pellet will be used for flow cytometry if required). If tube 3 is bloodstained, take an aliquot for microbiology.

    TUBE 2 is used for Cytology

    TUBE 3 is for Microbiological analysis (cell count, Gram stain and culture) and any further infectious testing (e.g. viral PCR, mycobacterial PCR and culture, cryptococcal antigen).

    TUBE 4 will be required only for;

    (a) Creutzfeld-Jacob Disease (CJD) testing OR

    (b) Xanthochromia analysis (wrap the tube in foil to prevent sample deterioration due to light).

     

    TESTS REQUIRED

    MINIMUM REQUIRED VOLUME (mL)

    TUBE 1

    TUBE 2

    TUBE 3

    TUBE 4

    CHEMISTRY

    1.0

         

    ADD FLOW CYTOMETRY

    Minimum 5.0-7.0

         

    CYTOLOGY

     

    1.0-3.0

       

    MICROBIOLOGY

       

    1.0-2.0

     

    ADD OTHER INFECTIOUS MARKERS

       

    add 2.0- 5.0-

     

    ADD MYCOBACTERIAL CULTURE

       

    Add 6.0-10.00

     

    ADD CJD

         

    1.0

    ADD XANTHOCHROMIA

         

    1.0 (foil)

     

    Note, the ability to add further tests to a previously collected sample is dependent upon the volume provided. Also, many tests (flow cytometry, cytology) can only be performed on fresh specimens.

     

    Other specialised testing (e.g. oligoclonal bands) will be performed using the supplied samples.

    Xanthochromia and CJD analysis cannot be performed on a blood-stained sample.


     Recent changes for Cerebrospinal Fluid Specimen Collection

    Recent changes for Cerebrospinal Fluid Specimen Collection

    Date
    Field
    Changed From
    Changed To
    27th April 2021
    Notes

    Depending on the tests requested, 3-4 separate tubes will need to be provided. Note that the tubes are numbered so that order of collection can be identified – this order should be carefully followed, so that the optimal sample can be used for the requested tests. It is also important to consider the tests that are required, as this will determine how much CSF is needed in each tube. See the matrix below for details.

    TUBE 1 is used for Biochemistry and Flow Cytometry. (Centrifuged in the lab – the supernatant is used for Biochemistry analysis (glucose, protein) and the cellular pellet will be used for flow cytometry if required). If tube 3 is bloodstained, take an aliquot for microbiology.

    TUBE 2 is used for Cytology

    TUBE 3 is for Microbiological analysis (cell count, Gram stain and culture) and any further infectious testing (e.g. viral PCR, mycobacterial PCR and culture, cryptococcal antigen).

    TUBE 4 will be required only for;

    (a) Creutzfeld-Jacob Disease (CJD) testing OR

    (b) Xanthochromia analysis (wrap the tube in foil to prevent sample deterioration due to light).

     

    TESTS REQUIRED

    MINIMUM REQUIRED VOLUME (mL)

    TUBE 1

    TUBE 2

    TUBE 3

    TUBE 4

    CHEMISTRY

    1.0

         

    ADD FLOW CYTOMETRY

    Minimum 5.0-7.0

         

    CYTOLOGY

     

    1.0-3.0

       

    MICROBIOLOGY

       

    1.0-2.0

     

    ADD OTHER INFECTIOUS MARKERS

       

    add 2.0- 5.0-

     

    ADD MYCOBACTERIAL CULTURE

       

    Add 6.0-10.00

     

    ADD CJD

         

    1.0

    ADD XANTHOCHROMIA

         

    1.0 (foil)

     

    Note, the ability to add further tests to a previously collected sample is dependent upon the volume provided. Also, many tests (flow cytometry, cytology) can only be performed on fresh specimens.

     

    Other specialised testing (e.g. oligoclonal bands) will be performed using the supplied samples.

    Xanthochromia and CJD analysis cannot be performed on a blood-stained sample.

     

     

    Depending on the tests requested, 3-4 separate tubes will need to be provided. Note that the tubes are numbered so that order of collection can be identified – this order should be carefully followed, so that the optimal sample can be used for the requested tests. It is also important to consider the tests that are required, as this will determine how much CSF is needed in each tube. See the matrix below for details.

    TUBE 1 is used for Biochemistry and Flow Cytometry. (Centrifuged in the lab – the supernatant is used for Biochemistry analysis (glucose, protein) and the cellular pellet will be used for flow cytometry if required). If tube 3 is bloodstained, take an aliquot for microbiology.

    TUBE 2 is used for Cytology

    TUBE 3 is for Microbiological analysis (cell count, Gram stain and culture) and any further infectious testing (e.g. viral PCR, mycobacterial PCR and culture, cryptococcal antigen).

    TUBE 4 will be required only for;

    (a) Creutzfeld-Jacob Disease (CJD) testing OR

    (b) Xanthochromia analysis (wrap the tube in foil to prevent sample deterioration due to light).

     

    TESTS REQUIRED

    MINIMUM REQUIRED VOLUME (mL)

    TUBE 1

    TUBE 2

    TUBE 3

    TUBE 4

    CHEMISTRY

    1.0

         

    ADD FLOW CYTOMETRY

    Minimum 5.0-7.0

         

    CYTOLOGY

     

    1.0-3.0

       

    MICROBIOLOGY

       

    1.0-2.0

     

    ADD OTHER INFECTIOUS MARKERS

       

    add 2.0- 5.0-

     

    ADD MYCOBACTERIAL CULTURE

       

    Add 6.0-10.00

     

    ADD CJD

         

    1.0

    ADD XANTHOCHROMIA

         

    1.0 (foil)

     

    Note, the ability to add further tests to a previously collected sample is dependent upon the volume provided. Also, many tests (flow cytometry, cytology) can only be performed on fresh specimens.

     

    Other specialised testing (e.g. oligoclonal bands) will be performed using the supplied samples.

    Xanthochromia and CJD analysis cannot be performed on a blood-stained sample.

     

    27th April 2021
    Notes

    CSF SPECIMEN INFORMATION – LABORATORY PROCESSING

    Depending on the tests requested, 3-4 separate tubes will need to be provided.  Note that the tubes are numbered so that order of collection can be identified – this order should be carefully followed, so that the optimal sample can be used for the requested tests.  It is also important to consider the tests that are required, as this will determine how much CSF is needed in each tube.  See the matrix below for details.

    TUBE 1 is used for Cytology.

    TUBE 2 is used for Glucose, Protein and Flow Cytometry.

    (centrifuged in the lab – the supernatant is used for Biochemistry analysis (glucose, protein) and the cellular pellet will be used for flow cytometry if required).

    TUBE 3 is for Microbiological analysis (cell count, Gram stain and culture) and any further infectious testing (e.g. viral PCR, mycobacterial PCR and culture, cryptococcal antigen).

    TUBE 4 will be required only for;

             (a) Creutzfeld-Jacob Disease (CJD) testing OR

    (b) Xanthochromia analysis (wrap the tube in foil to prevent    sample deterioration due to light).

     

    TESTS REQUIRED

    MINIMUM REQUIRED VOLUME (mL)

    TUBE 1

    TUBE 2

    TUBE 3

    TUBE 4

    CYTOLOGY. CHEMISTRY, MICRO/CULTURE

    1.0-2.0

    1.0

    1.0-2.0

    Nil

    ADD FLOW CYTOMETRY

     

    add 5.0-7.0

     

     

    ADD OTHER INFECTIOUS MARKERS

     

     

    add 2.0- 5.0-

     

    ADD MYCOBACTERIAL CULTURE

     

     

    Add 10.00

     

    ADD CJD

     

     

     

    1.0

    ADD XANTHOCHROMIA

     

     

     

    1.0 (foil)

     

    Note, the ability to add further tests to a previously collected sample is dependent upon the volume provided.  Also, many tests (flow cytometry, cytology) can only be performed on fresh specimens.

     

    Other specialised testing (e.g. oligoclonal bands) will be performed using the supplied samples.

     

    Xanthochromia and CJD analysis cannot be performed on a blood-stained sample.

    Depending on the tests requested, 3-4 separate tubes will need to be provided. Note that the tubes are numbered so that order of collection can be identified – this order should be carefully followed, so that the optimal sample can be used for the requested tests. It is also important to consider the tests that are required, as this will determine how much CSF is needed in each tube. See the matrix below for details.

    TUBE 1 is used for Biochemistry and Flow Cytometry. (Centrifuged in the lab – the supernatant is used for Biochemistry analysis (glucose, protein) and the cellular pellet will be used for flow cytometry if required). If tube 3 is bloodstained, take an aliquot for microbiology.

    TUBE 2 is used for Cytology

    TUBE 3 is for Microbiological analysis (cell count, Gram stain and culture) and any further infectious testing (e.g. viral PCR, mycobacterial PCR and culture, cryptococcal antigen).

    TUBE 4 will be required only for;

    (a) Creutzfeld-Jacob Disease (CJD) testing OR

    (b) Xanthochromia analysis (wrap the tube in foil to prevent sample deterioration due to light).

     

    TESTS REQUIRED

    MINIMUM REQUIRED VOLUME (mL)

    TUBE 1

    TUBE 2

    TUBE 3

    TUBE 4

    CHEMISTRY

    1.0

         

    ADD FLOW CYTOMETRY

    Minimum 5.0-7.0

         

    CYTOLOGY

     

    1.0-3.0

       

    MICROBIOLOGY

       

    1.0-2.0

     

    ADD OTHER INFECTIOUS MARKERS

       

    add 2.0- 5.0-

     

    ADD MYCOBACTERIAL CULTURE

       

    Add 6.0-10.00

     

    ADD CJD

         

    1.0

    ADD XANTHOCHROMIA

         

    1.0 (foil)

     

    Note, the ability to add further tests to a previously collected sample is dependent upon the volume provided. Also, many tests (flow cytometry, cytology) can only be performed on fresh specimens.

     

    Other specialised testing (e.g. oligoclonal bands) will be performed using the supplied samples.

    Xanthochromia and CJD analysis cannot be performed on a blood-stained sample.

     

     


     Recent changes for Coagulation Factor Assays II, V, VII, VIII, IX, X, XI, XII

    Recent changes for Coagulation Factor Assays II, V, VII, VIII, IX, X, XI, XII

    Date
    Field
    Changed From
    Changed To
    10th December 2020
    Collection & Request Instructions

    Do not use 1.0 mL citrate tubes

    Collect 1 X 3.5 ml Sodium Citrate tube is required to run all Factor assay ( Excent Factor XIII which is a refered test).

    1 Tube is sufficient for performing these tests.
     

    Do not use 1.0 mL citrate tubes

    Collect 1 X 3.5 ml Sodium Citrate tube is sufficient to run all Factor assay ( Excent Factor XIII which is a refered test).

    1 Tube is sufficient for performing these tests.
     

    10th December 2020
    Collection & Request Instructions

    Do not use 1.0 mL citrate tubes

    Do not use 1.0 mL citrate tubes

    Collect 1 X 3.5 ml Sodium Citrate tube is required to run all Factor assay ( Excent Factor XIII which is a refered test).

    1 Tube is sufficient for performing these tests.
     


     Recent changes for Collagen Binding Assay

    Recent changes for Collagen Binding Assay

    Date
    Field
    Changed From
    Changed To
    11th June 2021
    Laboratory

    Referred Test

    Coagulation

    Request Group

    CBA

    Collection & Request Instructions

    Fill to label marker and mix by inversion.

    Do not use 1 mL citrate tubes.

    Fill to label marker and mix by inversion.

    Do not use 1 mL citrate tubes.

    Processing Instructions

    Specimens kept at room temperature, should be centrifuged ASAP and tested within 4 hours from time of specimen collection. If testing is not completed within 4 hours, plasma should be removed from cells and aliquoted (1mL) into Eppendorf tubes, 2 X 1 mL vials per CBA assay.

    Plasma may be frozen @ -20 C for up to 2 weeks or -70 C for up to 6 months. Frost free freezers should not be used.

    Specimens kept at room temperature, should be centrifuged ASAP and tested within 4 hours from time of specimen collection. If testing is not completed within 4 hours, plasma should be removed from cells and aliquoted (1mL) into Eppendorf tubes, 2 X 1 mL vials per CBA assay.

    Plasma may be frozen @ -20 C for up to 2 weeks or -70 C for up to 6 months. Frost free freezers should not be used.

    External Laboratory

    Austin Pathology

    Frequency

    Fortnightly


     Recent changes for Copper-Liver

    Recent changes for Copper-Liver

    Date
    Field
    Changed From
    Changed To
    10th May 2021
    External Laboratory

    Australian Clinical Labs (formerly Healthscope)

    Pacific Laboratory Medicine Services


     Recent changes for COVID-19 Antibody

    Recent changes for COVID-19 Antibody

    Date
    Field
    Changed From
    Changed To
    24th September 2020
    Assay name

    covab

    COVAB

    Alternative names

    SARS-CoV-2 Antibody COVID Antibody COVID Serology

    Laboratory

    Unassigned

    Biochemistry

    Request Group

    COVAB

    Collection & Request Instructions

    Transport specimen at Room Temperature to lab

    Frequency

    Daily


     Recent changes for COVID-19 PCR

    Recent changes for COVID-19 PCR

    Date
    Field
    Changed From
    Changed To
    16th September 2020
    External Laboratory

    Victorian Infectious Diseases Reference Laboratory (V.I.D.R.L)

    16th September 2020
    Collection & Request Instructions

    Nasopharyngeal swab in RED Copan viral transport medium

    Nasal/oropharyngeal swab in GREEN Kang Jian viral transport medium

    Nasal/oropharyngeal swab in GREEN Kang Jian viral transport medium

    Paediatric - Nasopharyngeal swab in RED Copan viral transport medium

    Notes

    For Rapid testing from Emergency Department - swab must be accompanied by signed Rapid Test Authorisation Form.

    DO NOT call the laboratory for results/enquiries. Inpatient tests will be prioritised and results available on EMR ASAP.

    Sputum samples are referred to an external laboratory - results will be delayed.

    Samples MUST be in a clear specimen bag within an outer GREEN specimen bag

    DO NOT send samples in the Pathology Chute

    For Rapid testing from Emergency Department - swab must be accompanied by signed Rapid Test Authorisation Form.

    DO NOT call the laboratory for results/enquiries. Inpatient tests will be prioritised and results available on EMR ASAP.

    Sputum samples are referred to an external laboratory - results will be delayed.

    16th September 2020
    Preferred Specimen Type

    Nasopharyngeal swab
    NTS - nose & throat swab

    Nasopharyngeal swab
    NTS - nose & throat swab
    Sputum

    Preferred Container Type

    Viral Transport Media - Nasopharyngeal
    respiratory PCR requirements
    See Instructions

    Viral Transport Media - Nasopharyngeal
    See Instructions
    Sterile Jar

    Notes

    For Rapid testing from Emergency Department - swab must be accompanied by signed Rapid Test Authorisation Form.

    DO NOT call the laboratory for results/enquiries. Inpatient tests will be prioritised and results available on EMR.

    For Rapid testing from Emergency Department - swab must be accompanied by signed Rapid Test Authorisation Form.

    DO NOT call the laboratory for results/enquiries. Inpatient tests will be prioritised and results available on EMR ASAP.

    Sputum samples are referred to an external laboratory - results will be delayed.


     Recent changes for DNA analysis for Thalassaemia (related to Hgb Electrophoresis)

    Recent changes for DNA analysis for Thalassaemia (related to Hgb Electrophoresis)

    Date
    Field
    Changed From
    Changed To
    10th December 2020
    Collection & Request Instructions

    Send FBE, Iron studies and Hb electrophoresis results.

    Send within 24 Hrs.

    Results: 95943398

    Please collect 2x EDTA tube for this test.

    Send FBE, Iron studies and Hb electrophoresis results.

    Send within 24 Hrs.

    Results: 95943398


     Recent changes for Euglobulin Clot Lysis Time

    Recent changes for Euglobulin Clot Lysis Time

    Date
    Field
    Changed From
    Changed To
    10th December 2020
    Volume (Adults)

    Method

    This test is no longer performed. Please contact Haematologist or Senior Scientist in Haematology if further information is required.


     Recent changes for Fluconazole

    Recent changes for Fluconazole

    Date
    Field
    Changed From
    Changed To
    14th October 2021
    Collection & Request Instructions

     

     

     

     

    Processing Instructions

    Spin, separate Plasma and freeze.

    Packed by certified personnel.

    Results:02-83829100 or 02-82829190

    Spin and separate Plasma.

     

    External Laboratory

    Sydpath Pathology, St Vincent's Hospital NSW

    Alfred Hospital


     Recent changes for Free PSA

    Recent changes for Free PSA

    Date
    Field
    Changed From
    Changed To
    21st June 2021
    External Laboratory

    Melbourne Pathology

    Method

    Free PSA is now performed in house at Box Hill Hospital.

    Chemical Pathologist approval is still required


     Recent changes for Haemoglobin Analysis (including Electrophoresis)

    Recent changes for Haemoglobin Analysis (including Electrophoresis)

    Date
    Field
    Changed From
    Changed To
    10th December 2020
    Alternative names

    Alkali Resistant Haemoglobin, Foetal Haemoglobin, Hb EPG, HbA2, HbF, HbH, HbS, Unstable Haemoglobin

    Alkali Resistant Haemoglobin, Foetal Haemoglobin, Hb EPG, HbA2, HbF, HbH, HbS, Unstable Haemoglobin, Thalassaemia

    Collection & Request Instructions

    Please collect 2 X EDTA Tube for this test.


     Recent changes for Herpes Virus PCR

    Recent changes for Herpes Virus PCR

    Date
    Field
    Changed From
    Changed To
    19th October 2020
    External Transport Instructions

    Tests included in Monash PCR - Herpes simplex 1 virus, Herpes simplex 2 virus, Varicella zoster virus, Cytomegalovirus, Enterovirus (swabs only), Adenovirus (not faeces)

    Specimens tested at Monash-

    Swabs (dry or in UTM) serum, Plasma (ACD or EDTA), Sterile fluids, Respiratory samples, Urine.

    Specimens anticoagulated with heparin are unsuitable as heparin inhibits PCR

    For other specimens - refer to VIDRL. Tissue, CSF, saliva.

    If  CSF Herpes and Enterovirus PCR -send to VIDRL.

    Refer to Referred Molecular Test Requests WORK-MI-90

    Tests included in Monash PCR - Herpes simplex 1 virus, Herpes simplex 2 virus, Varicella zoster virus, Cytomegalovirus, Enterovirus (swabs only), Adenovirus (not faeces)

    Specimens tested at Monash-

    Swabs (dry or in UTM), Sterile fluids, Respiratory samples, Urine.

    Specimens anticoagulated with heparin are unsuitable as heparin inhibits PCR

    For other specimens - refer to VIDRL. Tissue, CSF, saliva, serum, Plasma (ACD or EDTA),

    If  CSF Herpes and Enterovirus PCR -send to VIDRL.

    Refer to Referred Molecular Test Requests WORK-MI-90


     Recent changes for Infectious Mononucleosis Screen

    Recent changes for Infectious Mononucleosis Screen

    Date
    Field
    Changed From
    Changed To
    30th March 2022
    Preferred Specimen Type

    Serum

    Serum
    Blood

    Optional Specimen Type

    Plasma

    Preferred Container Type

    Serum tube with gel

    Serum tube with gel
    EDTA
    EDTA - Paediatric


     Recent changes for Infliximab

    Recent changes for Infliximab

    Date
    Field
    Changed From
    Changed To
    5th April 2022
    Alternative names

    Inflectra, Remicade

    Volume (Adults)

    2 mL

    Processing Instructions

    After centifugation the serum must be aliquoted into the special aliquot tube provided and freeze.

    Store at 4 C.

     

    External Laboratory

    South Western Area Pathology,Liverpool Hospital

    Dorevitch Pathology

    External Transport Instructions

    Samples to be batched and sent  to SSWAPs frozen

    Send to Testing Laboratory at 4 C

    Frequency

    Monthly

    Weekly


     Recent changes for Iron - Liver

    Recent changes for Iron - Liver

    Date
    Field
    Changed From
    Changed To
    10th May 2021
    Collection & Request Instructions

    Liver biopsy specimen to be placed directly into a clear, dry container and delivered to the laboraory as soon as possible. Minimum sample size for accurate analysis is 1-2 mg.

    Liver biopsy specimen to be placed directly into a clear, dry container and delivered to the laboraory as soon as possible. Minimum sample size for accurate analysis is 1-2 mg.

    External Laboratory

    Australian Clinical Labs (formerly Healthscope)

    Pacific Laboratory Medicine Services

    Notes

    Hepatic Iron Index

    Hepatic Iron Concentration

     


     

    Age in years

    Values > 2 indicate haemochromatosis

    Liver biopsy specimen to be placed directly into a clear, dry container and delivered to the laboraory as soon as possible. Minimum sample size for accurate analysis is 1-2 mg.

     

    Hepatic Iron Index

    Hepatic Iron Concentration

     

     

    Age in years

    Values > 2 indicate haemochromatosis

    Liver biopsy specimen to be placed directly into a clear, dry container and delivered to the laboraory as soon as possible. Minimum sample size for accurate analysis is 1-2 mg.


     Recent changes for Itraconazole

    Recent changes for Itraconazole

    Date
    Field
    Changed From
    Changed To
    14th July 2022
    Volume (Adults)

    2 mL

    5 mL

    Minimum/Paediatric Volume

    1 mL

    Preferred Container Type

    Lithium heparin no gel

    EDTA

    Processing Instructions

    Spin, separate and freeze plasma.

    Spin and separate plasma.

    External Laboratory

    Women's & Children's Hospital (Adelaide)

    Alfred Hospital

    External Transport Instructions

    Packed by certified personnal - transport frozen.

    Results : 08-81617000, 08-81616732.

    Frequency

    Monthly

    Twice weekly

    Notes

    Dose information should be provided and length of therapy must be >5 days.


     Recent changes for Karyotype/Microarray - TISSUE

    Recent changes for Karyotype/Microarray - TISSUE

    Date
    Field
    Changed From
    Changed To
    6th March 2022
    Assay name

    Cytogenetics / Karyotype - TISSUE

    Karyotype/Microarray - TISSUE

    14th December 2021
    Assay name

    CytogeneticsKaryotype - TISSUE

    Cytogenetics / Karyotype - TISSUE

    14th December 2021
    Assay name

    CYTOGENETICS - TISSUE

    CytogeneticsKaryotype - TISSUE

    Alternative names

    KARYOTYPE CHROMOSOME STUDIES - TISSUE TISSUE CULTURE MOLECULAR KARYOTYPE MICROARRAY

    KARYOTYPE CHROMOSOME STUDIES - TISSUE TISSUE CULTURE MOLECULAR KARYOTYPE MICROARRAY FIBROBLAST CULTURE

    14th December 2021
    Assay name

    CYTOGENETICS - TISSUE NON MALIGNANT (CONSTITUTIONAL)

    CYTOGENETICS - TISSUE

    Frequency

    Weekly

    Daily


     Recent changes for Lamotrigine

    Recent changes for Lamotrigine

    Date
    Field
    Changed From
    Changed To
    14th July 2022
    Alternative names

    Lamictal

    Volume (Adults)

    2 mL

    3 mL

    Minimum/Paediatric Volume

    1 mL

    3 mL

    Processing Instructions

    Spin aliquot plasma. (Send either plasma or whole blood)

    Spin & aliquot plasma

    External Laboratory

    Australian Clinical Labs (formerly Healthscope)

    Alfred Hospital


     Recent changes for Leucocyte Immunophenotyping

    Recent changes for Leucocyte Immunophenotyping

    Date
    Field
    Changed From
    Changed To
    15th October 2021
    Notes

    Samples must be marked 'URGENT', must reach the laboratory as soon as possible. 

    All out-of-hours testing requires notification.

    Time and date of collection must be recorded on request form.

    Whole blood - 1 x EDTA sample, do not spin. Store at room temperature. An FBE must also be requested,  and needs a separate EDTA sample.

    Bone marrow aspirate - Sample into  EDTA tube

    TissueFresh (if to be shared with other departments for other tests eg. Histopathology or Microbiology) or RPMI fluid (especially if specimen is taken out of hours). This fluid is obtainable from: BH Lab 9895 3884, MH Lab: 9871 3572 or AH Lab: 9764 6136.

    CSF/other fluids

    Depending on the tests requested, 3-4 separate tubes will need to be provided. Note that the tubes are numbered so that order of collection can be identified – this order should be carefully followed, so that the optimal sample can be used for the requested tests. It is also important to consider the tests that are required, as this will determine how much CSF is needed in each tube. See the matrix below for details.

    TUBE 1 is used for Biochemistry and Flow Cytometry. (Centrifuged in the lab – the supernatant is used for Biochemistry analysis (glucose, protein) and the cellular pellet will be used for flow cytometry if required). If tube 3 is bloodstained, take an aliquot for microbiology.

    TUBE 2 is used for Cytology

    TUBE 3 is for Microbiological analysis (cell count, Gram stain and culture) and any further infectious testing (e.g. viral PCR, mycobacterial PCR and culture, cryptococcal antigen).

    TUBE 4 will be required only for;

    (a) Creutzfeld-Jacob Disease (CJD) testing OR

    (b) Xanthochromia analysis (wrap the tube in foil to prevent sample deterioration due to light).

     

    TESTS REQUIRED

    MINIMUM REQUIRED VOLUME (mL)

    TUBE 1

    TUBE 2

    TUBE 3

    TUBE 4

    CHEMISTRY

    1.0

     

     

     

    ADD FLOW CYTOMETRY

    Minimum 5.0-7.0

     

     

     

    CYTOLOGY

     

    1.0-3.0

     

     

    MICROBIOLOGY

     

     

    1.0-2.0

     

    ADD OTHER INFECTIOUS MARKERS

     

     

    add 2.0- 5.0-

     

    ADD MYCOBACTERIAL CULTURE

     

     

    Add 6.0-10.00

     

    ADD CJD

     

     

     

    1.0

    ADD XANTHOCHROMIA

     

     

     

    1.0 (foil)

     

    Note, the ability to add further tests to a previously collected sample is dependent upon the volume provided. Also, many tests (flow cytometry, cytology) can only be performed on fresh specimens.

     

    Other specialised testing (e.g. oligoclonal bands) will be performed using the supplied samples.

    Xanthochromia and CJD analysis cannot be performed on a blood-stained sample

    Samples must be marked 'URGENT', must reach the laboratory as soon as possible. 

    All out-of-hours testing requires notification.

    Time and date of collection must be recorded on request form.

    Whole blood - 1 x EDTA sample, do not spin. Store at room temperature. An FBE and BLOOD FILM must also be requested,  and needs a separate EDTA sample.

    Bone marrow aspirate - Sample into  EDTA tube

    TissueFresh (if to be shared with other departments for other tests eg. Histopathology or Microbiology) or RPMI fluid (especially if specimen is taken out of hours). This fluid is obtainable from: BH Lab 9895 3884, MH Lab: 9871 3572 or AH Lab: 9764 6136.

    CSF/other fluids

    Depending on the tests requested, 3-4 separate tubes will need to be provided. Note that the tubes are numbered so that order of collection can be identified – this order should be carefully followed, so that the optimal sample can be used for the requested tests. It is also important to consider the tests that are required, as this will determine how much CSF is needed in each tube. See the matrix below for details.

    TUBE 1 is used for Biochemistry and Flow Cytometry. (Centrifuged in the lab – the supernatant is used for Biochemistry analysis (glucose, protein) and the cellular pellet will be used for flow cytometry if required). If tube 3 is bloodstained, take an aliquot for microbiology.

    TUBE 2 is used for Cytology

    TUBE 3 is for Microbiological analysis (cell count, Gram stain and culture) and any further infectious testing (e.g. viral PCR, mycobacterial PCR and culture, cryptococcal antigen).

    TUBE 4 will be required only for;

    (a) Creutzfeld-Jacob Disease (CJD) testing OR

    (b) Xanthochromia analysis (wrap the tube in foil to prevent sample deterioration due to light).

     

    TESTS REQUIRED

    MINIMUM REQUIRED VOLUME (mL)

    TUBE 1

    TUBE 2

    TUBE 3

    TUBE 4

    CHEMISTRY

    1.0

     

     

     

    ADD FLOW CYTOMETRY

    Minimum 5.0-7.0

     

     

     

    CYTOLOGY

     

    1.0-3.0

     

     

    MICROBIOLOGY

     

     

    1.0-2.0

     

    ADD OTHER INFECTIOUS MARKERS

     

     

    add 2.0- 5.0-

     

    ADD MYCOBACTERIAL CULTURE

     

     

    Add 6.0-10.00

     

    ADD CJD

     

     

     

    1.0

    ADD XANTHOCHROMIA

     

     

     

    1.0 (foil)

     

    Note, the ability to add further tests to a previously collected sample is dependent upon the volume provided. Also, many tests (flow cytometry, cytology) can only be performed on fresh specimens.

     

    Other specialised testing (e.g. oligoclonal bands) will be performed using the supplied samples.

    Xanthochromia and CJD analysis cannot be performed on a blood-stained sample

    14th October 2021
    Notes

     

     

    Samples must be marked 'URGENT', must reach the laboratory as soon as possible. 

    All out-of-hours testing requires notification.

    Time and date of collection must be recorded on request form.

    Whole blood - 1 x EDTA sample, do not spin. Store at room temperature. An FBE must also be requested,  and needs a separate EDTA sample.

    Bone marrow aspirate - Sample into  EDTA tube

    TissueFresh (if to be shared with other departments for other tests eg. Histopathology or Microbiology) or RPMI fluid (especially if specimen is taken out of hours). This fluid is obtainable from: BH Lab 9895 3884, MH Lab: 9871 3572 or AH Lab: 9764 6136.

    CSF/other fluids - at least 2mL is preferred

    Depending on the tests requested, 3-4 separate tubes will need to be provided. Note that the tubes are numbered so that order of collection can be identified – this order should be carefully followed, so that the optimal sample can be used for the requested tests. It is also important to consider the tests that are required, as this will determine how much CSF is needed in each tube. See the matrix below for details.

    TUBE 1 is used for Biochemistry and Flow Cytometry. (Centrifuged in the lab – the supernatant is used for Biochemistry analysis (glucose, protein) and the cellular pellet will be used for flow cytometry if required). If tube 3 is bloodstained, take an aliquot for microbiology.

    TUBE 2 is used for Cytology

    TUBE 3 is for Microbiological analysis (cell count, Gram stain and culture) and any further infectious testing (e.g. viral PCR, mycobacterial PCR and culture, cryptococcal antigen).

    TUBE 4 will be required only for;

    (a) Creutzfeld-Jacob Disease (CJD) testing OR

    (b) Xanthochromia analysis (wrap the tube in foil to prevent sample deterioration due to light).

     

    TESTS REQUIRED

    MINIMUM REQUIRED VOLUME (mL)

    TUBE 1

    TUBE 2

    TUBE 3

    TUBE 4

    CHEMISTRY

    1.0

     

     

     

    ADD FLOW CYTOMETRY

    Minimum 5.0-7.0

     

     

     

    CYTOLOGY

     

    1.0-3.0

     

     

    MICROBIOLOGY

     

     

    1.0-2.0

     

    ADD OTHER INFECTIOUS MARKERS

     

     

    add 2.0- 5.0-

     

    ADD MYCOBACTERIAL CULTURE

     

     

    Add 6.0-10.00

     

    ADD CJD

     

     

     

    1.0

    ADD XANTHOCHROMIA

     

     

     

    1.0 (foil)

     

    Note, the ability to add further tests to a previously collected sample is dependent upon the volume provided. Also, many tests (flow cytometry, cytology) can only be performed on fresh specimens.

     

    Other specialised testing (e.g. oligoclonal bands) will be performed using the supplied samples.

    Xanthochromia and CJD analysis cannot be performed on a blood-stained sample

     

     

    Samples must be marked 'URGENT', must reach the laboratory as soon as possible. 

    All out-of-hours testing requires notification.

    Time and date of collection must be recorded on request form.

    Whole blood - 1 x EDTA sample, do not spin. Store at room temperature. An FBE must also be requested,  and needs a separate EDTA sample.

    Bone marrow aspirate - Sample into  EDTA tube

    TissueFresh (if to be shared with other departments for other tests eg. Histopathology or Microbiology) or RPMI fluid (especially if specimen is taken out of hours). This fluid is obtainable from: BH Lab 9895 3884, MH Lab: 9871 3572 or AH Lab: 9764 6136.

    CSF/other fluids

    Depending on the tests requested, 3-4 separate tubes will need to be provided. Note that the tubes are numbered so that order of collection can be identified – this order should be carefully followed, so that the optimal sample can be used for the requested tests. It is also important to consider the tests that are required, as this will determine how much CSF is needed in each tube. See the matrix below for details.

    TUBE 1 is used for Biochemistry and Flow Cytometry. (Centrifuged in the lab – the supernatant is used for Biochemistry analysis (glucose, protein) and the cellular pellet will be used for flow cytometry if required). If tube 3 is bloodstained, take an aliquot for microbiology.

    TUBE 2 is used for Cytology

    TUBE 3 is for Microbiological analysis (cell count, Gram stain and culture) and any further infectious testing (e.g. viral PCR, mycobacterial PCR and culture, cryptococcal antigen).

    TUBE 4 will be required only for;

    (a) Creutzfeld-Jacob Disease (CJD) testing OR

    (b) Xanthochromia analysis (wrap the tube in foil to prevent sample deterioration due to light).

     

    TESTS REQUIRED

    MINIMUM REQUIRED VOLUME (mL)

    TUBE 1

    TUBE 2

    TUBE 3

    TUBE 4

    CHEMISTRY

    1.0

     

     

     

    ADD FLOW CYTOMETRY

    Minimum 5.0-7.0

     

     

     

    CYTOLOGY

     

    1.0-3.0

     

     

    MICROBIOLOGY

     

     

    1.0-2.0

     

    ADD OTHER INFECTIOUS MARKERS

     

     

    add 2.0- 5.0-

     

    ADD MYCOBACTERIAL CULTURE

     

     

    Add 6.0-10.00

     

    ADD CJD

     

     

     

    1.0

    ADD XANTHOCHROMIA

     

     

     

    1.0 (foil)

     

    Note, the ability to add further tests to a previously collected sample is dependent upon the volume provided. Also, many tests (flow cytometry, cytology) can only be performed on fresh specimens.

     

    Other specialised testing (e.g. oligoclonal bands) will be performed using the supplied samples.

    Xanthochromia and CJD analysis cannot be performed on a blood-stained sample

    14th October 2021
    Alternative names

    CD4/CD8 Counts, Cell Surface Markers, FACS Analysis, Flow Cytometry

    Cell Surface Markers, FACS Analysis, Flow Cytometry, Immunophenotyping

    Request Group

    FLOW

    LYMP, LEUK

    Collection & Request Instructions

    Sample store at Room temperature. Samples are not stable for more than 24 hours. Hence they must be received by the Flow Cytometry lab at Box Hill within normal business hours Monday to Friday.

    Cutoff for outpatient sample collection on Fridays is at 11.30 AM and MUST reach the laboratory before 4.00PM.

    Flow cytometric analysis of cells stained by immunofluorescence with a range of antibodies against lymphoid and myeloid markers.

    The panel of markers used will be appropriate to the clinical comments supplied , or as arranged by prior consultation.

    Sample store at Room temperature. Samples are not stable for more than 24 hours. Hence they must be received by the Flow Cytometry lab at Box Hill within normal business hours Monday to Friday.

    Cut-off for outpatient sample collection on Fridays is at 11.30 AM and MUST reach the laboratory before 4.00PM.

    Flow cytometric analysis of cells stained by immunofluorescence with a range of antibodies against lymphoid and myeloid markers.

    The panel of markers used will be appropriate to the panel selected and the clinical comments supplied , or as arranged by prior consultation

    Notes

     

     

    Samples must be marked 'URGENT', must reach the laboratory as soon as possible. 

    All out-of-hours testing requires notification.

    Time and date of collection must be recorded on request form.

    Whole blood - 1 x EDTA sample, do not spin. Store at room temperature. An FBE must also be requested, needs a separate EDTA sample.

    Bone marrow aspirate - Sample into  EDTA tube

    TissueFresh (if to be shared with other departments for other tests eg. Histopathology or Microbiology) or RPMI fluid (especially if specimen is taken out of hours). This fluid is obtainable from: BH Lab 9895 3884, MH Lab: 9871 3572 or AH Lab: 9764 6136.

    CSF/other fluids - at least 2mL is preferred

    Collect CSF into three separate, sterile, screw-capped tubes provided (labelled 1,2 & 3)

    Tube 1 is for Biochemistry (for Protein and Glucose) 0.5ml (10 drops) required 

    Tube 2 for Cytology 1.0ml (20 drops)  and/or Flow Cytometry (6ml)

    For Flow Cytometry a minimum of 6ml is required  in Tube 2

    For Flow Cytometry and Cytology a minimum of 7ml is required in Tube 2     

    Tube 3 for Microbiology upto 10ml (Full tube)

    If querying CJD the laboratory requires a 4th unopened tube containing at least 1ml of non-bloodstained CSF.

    Xanthochromia investigation requires a 4th tube containing 1ml of CSF .

     

     

    Samples must be marked 'URGENT', must reach the laboratory as soon as possible. 

    All out-of-hours testing requires notification.

    Time and date of collection must be recorded on request form.

    Whole blood - 1 x EDTA sample, do not spin. Store at room temperature. An FBE must also be requested,  and needs a separate EDTA sample.

    Bone marrow aspirate - Sample into  EDTA tube

    TissueFresh (if to be shared with other departments for other tests eg. Histopathology or Microbiology) or RPMI fluid (especially if specimen is taken out of hours). This fluid is obtainable from: BH Lab 9895 3884, MH Lab: 9871 3572 or AH Lab: 9764 6136.

    CSF/other fluids - at least 2mL is preferred

    Depending on the tests requested, 3-4 separate tubes will need to be provided. Note that the tubes are numbered so that order of collection can be identified – this order should be carefully followed, so that the optimal sample can be used for the requested tests. It is also important to consider the tests that are required, as this will determine how much CSF is needed in each tube. See the matrix below for details.

    TUBE 1 is used for Biochemistry and Flow Cytometry. (Centrifuged in the lab – the supernatant is used for Biochemistry analysis (glucose, protein) and the cellular pellet will be used for flow cytometry if required). If tube 3 is bloodstained, take an aliquot for microbiology.

    TUBE 2 is used for Cytology

    TUBE 3 is for Microbiological analysis (cell count, Gram stain and culture) and any further infectious testing (e.g. viral PCR, mycobacterial PCR and culture, cryptococcal antigen).

    TUBE 4 will be required only for;

    (a) Creutzfeld-Jacob Disease (CJD) testing OR

    (b) Xanthochromia analysis (wrap the tube in foil to prevent sample deterioration due to light).

     

    TESTS REQUIRED

    MINIMUM REQUIRED VOLUME (mL)

    TUBE 1

    TUBE 2

    TUBE 3

    TUBE 4

    CHEMISTRY

    1.0

     

     

     

    ADD FLOW CYTOMETRY

    Minimum 5.0-7.0

     

     

     

    CYTOLOGY

     

    1.0-3.0

     

     

    MICROBIOLOGY

     

     

    1.0-2.0

     

    ADD OTHER INFECTIOUS MARKERS

     

     

    add 2.0- 5.0-

     

    ADD MYCOBACTERIAL CULTURE

     

     

    Add 6.0-10.00

     

    ADD CJD

     

     

     

    1.0

    ADD XANTHOCHROMIA

     

     

     

    1.0 (foil)

     

    Note, the ability to add further tests to a previously collected sample is dependent upon the volume provided. Also, many tests (flow cytometry, cytology) can only be performed on fresh specimens.

     

    Other specialised testing (e.g. oligoclonal bands) will be performed using the supplied samples.

    Xanthochromia and CJD analysis cannot be performed on a blood-stained sample

    20th July 2021
    Assay name

    Leucocyte Immunophenotyping

    T Cell Subsets

    Alternative names

    CD4/CD8 Counts, Cell Surface Markers, FACS Analysis, Flow Cytometry

    CD2, CD3, CD4, CD8

    Preferred Container Type

    EDTA
    Sterile Jar

    EDTA
    Lithium heparin no gel

    Collection & Request Instructions

    Sample store at Room temperature. Samples are not stable for more than 24 hours. Hence they must be received by the Flow Cytometry lab at Box Hill within normal business hours Monday to Friday.

    Cutoff for outpatient sample collection on Fridays is at 11.30 AM and MUST reach the laboratory before 4.00PM.

    Flow cytometric analysis of cells stained by immunofluorescence with a range of antibodies against lymphoid and myeloid markers.

    The panel of markers used will be appropriate to the clinical comments supplied , or as arranged by prior consultation.

    The samples are not stable for more than 24 hours. Hence all samples must be recieved in the laboratory at Box Hill  Monday to Friday within business hours.

    Cutoff for outpatient sample collection on Fridays is at 11.30 AM and MUST reach the laboratory before 4.00PM.

    Processing Instructions

    Prefer EDTA sample.

    Do not spin, store at room temperature.

    Notes

     

     

    Samples must be marked 'URGENT', must reach the laboratory as soon as possible. 

    All out-of-hours testing requires notification.

    Time and date of collection must be recorded on request form.

    Whole blood - 1 x EDTA sample, do not spin. Store at room temperature. An FBE must also be requested, needs a separate EDTA sample.

    Bone marrow aspirate - Sample into  EDTA tube

    TissueFresh (if to be shared with other departments for other tests eg. Histopathology or Microbiology) or RPMI fluid (especially if specimen is taken out of hours). This fluid is obtainable from: BH Lab 9895 3884, MH Lab: 9871 3572 or AH Lab: 9764 6136.

    CSF/other fluids - at least 2mL is preferred

    Collect CSF into three separate, sterile, screw-capped tubes provided (labelled 1,2 & 3)

    Tube 1 is for Biochemistry (for Protein and Glucose) 0.5ml (10 drops) required 

    Tube 2 for Cytology 1.0ml (20 drops)  and/or Flow Cytometry (6ml)

    For Flow Cytometry a minimum of 6ml is required  in Tube 2

    For Flow Cytometry and Cytology a minimum of 7ml is required in Tube 2     

    Tube 3 for Microbiology upto 10ml (Full tube)

    If querying CJD the laboratory requires a 4th unopened tube containing at least 1ml of non-bloodstained CSF.

    Xanthochromia investigation requires a 4th tube containing 1ml of CSF .

    Used to monitor T-cell numbers

    Preferred EDTA sample. Do not spin, store at room temperature.

    19th July 2021
    Collection & Request Instructions

    Sample store at Room temperature. Samples are not stable for more than 24 hours. Hence they must be recieved by the Flow Cytometry lab at Box Hill within normal business hours Monday to Friday.

    Cutoff for outpatirnt sample collection on Fridays is at 12:00 PM and MUST reach the laboratory by 4.00PM.

    Flow cytometric analysis of cells stained by immunofluorescence with a range of antibodies against lymphoid and myeloid markers.

    The panel of markers used will be appropriate to the clinical comments supplied , or as arranged by prior consultation.

    Sample store at Room temperature. Samples are not stable for more than 24 hours. Hence they must be received by the Flow Cytometry lab at Box Hill within normal business hours Monday to Friday.

    Cutoff for outpatient sample collection on Fridays is at 11.30 AM and MUST reach the laboratory before 4.00PM.

    Flow cytometric analysis of cells stained by immunofluorescence with a range of antibodies against lymphoid and myeloid markers.

    The panel of markers used will be appropriate to the clinical comments supplied , or as arranged by prior consultation.

    19th July 2021
    Collection & Request Instructions

    Sample store at Room temperature. Samples are not stable for more than 24 hours. Hence they must be recieved by the Flow Cytometry lab at Box Hill within normal business hours Monday to Friday

    Flow cytometric analysis of cells stained by immunofluorescence with a range of antibodies against lymphoid and myeloid markers.

    The panel of markers used will be appropriate to the clinical comments supplied , or as arranged by prior consultation.

    Sample store at Room temperature. Samples are not stable for more than 24 hours. Hence they must be recieved by the Flow Cytometry lab at Box Hill within normal business hours Monday to Friday.

    Cutoff for outpatirnt sample collection on Fridays is at 12:00 PM and MUST reach the laboratory by 4.00PM.

    Flow cytometric analysis of cells stained by immunofluorescence with a range of antibodies against lymphoid and myeloid markers.

    The panel of markers used will be appropriate to the clinical comments supplied , or as arranged by prior consultation.

    Notes

     

     

    • Samples must be marked 'URGENT', must reach the laboratory as soon as possible. 
    • All out-of-hours testing requires notification.
    • Time and date of collection must be recorded on request form.

      Whole blood - 1 x EDTA sample, do not spin. Store at room temperature. An FBE must also be requested, needs a separate EDTA sample.

      Bone marrow aspirate - Sample into  EDTA tube

      TissueFresh (if to be shared with other departments for other tests eg. Histopathology or Microbiology) or RPMI fluid (especially if specimen is taken out of hours). This fluid is obtainable from: BH Lab 9895 3884, MH Lab: 9871 3572 or AH Lab: 9764 6136.

    • CSF/other fluids - at least 2mL is preferred

    • Collect CSF into three separate, sterile, screw-capped tubes provided (labelled 1,2 & 3)

    • Tube 1 is for Biochemistry (for Protein and Glucose) 0.5ml (10 drops) required 
    • Tube 2 for Cytology 1.0ml (20 drops)  and/or Flow Cytometry (6ml)
      • For Flow Cytometry a minimum of 6ml is required  in Tube 2
      • For Flow Cytometry and Cytology a minimum of 7ml is required in Tube 2     
    • Tube 3 for Microbiology upto 10ml (Full tube)

      If querying CJD the laboratory requires a 4th unopened tube containing at least 1ml of non-bloodstained CSF.

      Xanthochromia investigation requires a 4th tube containing 1ml of CSF .

     

     

    Samples must be marked 'URGENT', must reach the laboratory as soon as possible. 

    All out-of-hours testing requires notification.

    Time and date of collection must be recorded on request form.

    Whole blood - 1 x EDTA sample, do not spin. Store at room temperature. An FBE must also be requested, needs a separate EDTA sample.

    Bone marrow aspirate - Sample into  EDTA tube

    TissueFresh (if to be shared with other departments for other tests eg. Histopathology or Microbiology) or RPMI fluid (especially if specimen is taken out of hours). This fluid is obtainable from: BH Lab 9895 3884, MH Lab: 9871 3572 or AH Lab: 9764 6136.

    CSF/other fluids - at least 2mL is preferred

    Collect CSF into three separate, sterile, screw-capped tubes provided (labelled 1,2 & 3)

    Tube 1 is for Biochemistry (for Protein and Glucose) 0.5ml (10 drops) required 

    Tube 2 for Cytology 1.0ml (20 drops)  and/or Flow Cytometry (6ml)

    For Flow Cytometry a minimum of 6ml is required  in Tube 2

    For Flow Cytometry and Cytology a minimum of 7ml is required in Tube 2     

    Tube 3 for Microbiology upto 10ml (Full tube)

    If querying CJD the laboratory requires a 4th unopened tube containing at least 1ml of non-bloodstained CSF.

    Xanthochromia investigation requires a 4th tube containing 1ml of CSF .


     Recent changes for Linezolid

    Recent changes for Linezolid

    Date
    Field
    Changed From
    Changed To
    14th July 2022
    Volume (Adults)

    2 mL

    3 mL

    Minimum/Paediatric Volume

    3 mL

    Optional Container Type

    Lithium heparin no gel

    Collection & Request Instructions

    Deliver to laboratory ASAP.

    A separate unopened specimen is required for this test.

    Processing Instructions

    Spin, separate and freeze plasma.

    External Laboratory

    Sydpath Pathology, St Vincent's Hospital NSW

    Alfred Hospital

    External Transport Instructions

    Send at 4 C.

    Frequency

    As required

    Weekly

    Method

    Results: Clinical Pharmacology on 02 8382 9184.


     Recent changes for Lupus Anticoagulant Screen

    Recent changes for Lupus Anticoagulant Screen

    Date
    Field
    Changed From
    Changed To
    3rd May 2021
    External Transport Instructions

    Samples can be transported frozen to the laboratory if the facilities are available to double spin and aliquot these sample. This has to be done within 4 hours.

    Unspun sample is stable for up to 4 hours and must be transport to laboratory immediately. (Sample must be double spun, aliquoted and frozen within 4 hours from time of collection).

    Samples can be transported frozen to the laboratory if the facilities are available to double spin and aliquot these samples. This has to be performed within 4 hours of collection.

    Unspun sample is stable for up to 4 hours and must be transport to laboratory immediately. (Sample must be double spun, aliquoted and frozen within 4 hours from time of collection).

    3rd May 2021
    Collection & Request Instructions

    Notification of anticoagulant therapy is essential

    One (1x) Sodium citrate tubes is required. Do not use 1 ml citrate tubes.

    Filled to label marker and mixed by inversion

    The patient must not be receiving heparin

    Transport to laboratory immediately. (Sample must be double spun and frozen within 4 hours from time of collection).

    Tests performed:

    INR, APTT, APTT Mix, Silica Clotting Time and Dilute Russell's Viper venom time (LA screen/Confirm).

     

    Notification of anticoagulant therapy is essential

    One (1x) Sodium citrate tubes is required. Do not use 1 ml citrate tubes.

    Filled to label marker and mixed by inversion

    The patient must not be receiving heparin

    Unspun sample is stable for up to 4 hours and must be transport to laboratory immediately. (Sample must be double spun, aliquoted and frozen within 4 hours from time of collection).

     

    Tests performed:

    INR, APTT, APTT Mix, Silica Clotting Time and Dilute Russell's Viper venom time (LA screen/Confirm).

     

    Processing Instructions

    Transport sample to laboratory within 4 hours of collection.

    The sample MUST be double spun and frozen within the 4 hour time limit.

    Unspun sample is stable for up to 4 hours and must be transport to laboratory immediately. (Sample must be double spun, aliquoted and frozen within 4 hours from time of collection).

    External Transport Instructions

    Samples can be transported frozen to the laboratory if the facilities are available to double spin and aliquot these sample. This has to be done within 4 hours.

    Unspun sample is stable for up to 4 hours and must be transport to laboratory immediately. (Sample must be double spun, aliquoted and frozen within 4 hours from time of collection).

    3rd May 2021
    Collection & Request Instructions

    Notification of anticoagulant therapy is essential

    One (1x) Sodium citrate tubes is required. Do not use 1 ml citrate tubes.

    Filled to label marker and mixed by inversion

    The patient must not be receiving heparin

    Transport to laboratory immediately. (maximum of 4 hours from time of collection).

    Tests performed:

    INR, APTT, APTT Mix, Silica Clotting Time and Dilute Russell's Viper venom time (LA screen/Confirm).

     

    Notification of anticoagulant therapy is essential

    One (1x) Sodium citrate tubes is required. Do not use 1 ml citrate tubes.

    Filled to label marker and mixed by inversion

    The patient must not be receiving heparin

    Transport to laboratory immediately. (Sample must be double spun and frozen within 4 hours from time of collection).

    Tests performed:

    INR, APTT, APTT Mix, Silica Clotting Time and Dilute Russell's Viper venom time (LA screen/Confirm).

     

    Processing Instructions

    Transport sample to laboratory within 4 hours of collection.

    The sample MUST be double spun and frozen within the 4 hour time limit.

    19th January 2021
    Method

    Centrifuge the two 3.5 mL citrate tubes and transfer the plasma to a labeled 4 mL plastic tube and re-centrifuge at room temperature (do not use the refrigerated centrifuge).

    The double centrifuged plasma is transferred to two 1 mL eppendorf tubes and freeze.

    Sample must be centrifuged within 4 hours of collection.

    **Sample must be centrifuged within 4 hours of collection**

    Centrifuge the two 3.5 mL citrate tubes (and transfer the plasma to a labeled 4 mL plastic tube and re-centrifuge at room temperature (do not use the refrigerated centrifuge).  One sample is acceptable but 2 samples are preferable for Lupus testing.

    The double centrifuged plasma is transferred into a new labelled tubes. 

    Double spun samples are then frozen in the -20'C freezer.

    19th January 2021
    Collection & Request Instructions

    Notification of anticoagulant therapy is essential

    One (1x) Sodium citrate tubes is required. Do not use 1 ml citrate tubes.

    Filled to label marker and mixed by inversion

    The patient must not be receiving heparin

    Transport to laboratory immediately.

    Tests performed:

    INR, APTT, APTT Mix, Lupus sensitive APTT and Dilute Russell's Viper venom time (LA screen/Confirm).

     

    Notification of anticoagulant therapy is essential

    One (1x) Sodium citrate tubes is required. Do not use 1 ml citrate tubes.

    Filled to label marker and mixed by inversion

    The patient must not be receiving heparin

    Transport to laboratory immediately. (maximum of 4 hours from time of collection).

    Tests performed:

    INR, APTT, APTT Mix, Silica Clotting Time and Dilute Russell's Viper venom time (LA screen/Confirm).

     

    Method

    Centrifuge the two 3.5 mL citrate tubes and transfer the plasma to a labeled 4 mL plastic tube and re-centrifuge at room temperature (do not use the refrigerated centrifuge).

    The double centrifuged plasma is transferred to two 1 mL eppendorf tubes and freeze.

    Centrifuge the two 3.5 mL citrate tubes and transfer the plasma to a labeled 4 mL plastic tube and re-centrifuge at room temperature (do not use the refrigerated centrifuge).

    The double centrifuged plasma is transferred to two 1 mL eppendorf tubes and freeze.

    Sample must be centrifuged within 4 hours of collection.

    10th December 2020
    Collection & Request Instructions

    Notification of anticoagulant therapy is essential

    Two (2x) Sodium citrate tubes required. Do not use 1 ml citrate tubes.

    Filled to label marker and mixed by inversion

    The patient must not be receiving heparin

    Transport to laboratory immediately.

    Tests performed:

    INR, APTT, APTT Mix, Lupus sensitive APTT and Dilute Russell's Viper venom time (LA screen/Confirm).

     

    Notification of anticoagulant therapy is essential

    One (1x) Sodium citrate tubes is required. Do not use 1 ml citrate tubes.

    Filled to label marker and mixed by inversion

    The patient must not be receiving heparin

    Transport to laboratory immediately.

    Tests performed:

    INR, APTT, APTT Mix, Lupus sensitive APTT and Dilute Russell's Viper venom time (LA screen/Confirm).

     


     Recent changes for Lymphocyte subsets

    Recent changes for Lymphocyte subsets

    Date
    Field
    Changed From
    Changed To
    14th October 2021
    Alternative names

    CD3 count, CD4 count, CD8 count, CD4:CD8 ratio CD19 count, CD56 count, flow cytometry

    14th October 2021
    Alternative names

    CD19 count & CD56 count, CD4 count, CD4 count, CD4:CD8 ratio Lymphocyte subsets include: CD3 count, CD4:CD8 ratio, CD8 count, CD8 count, T subsets include: CD3 count

    T subsets include: CD3 count, CD4 count, CD8 count, CD4:CD8 ratio Lymphocyte subsets include: CD3 count, CD4 count, CD8 count, CD4:CD8 ratio, CD19 count & CD56 count Flow Cytometry

    Collection & Request Instructions

    Sample store at Room temperature. Samples are not stable for more than 24 hours. Hence they must be received by the Flow Cytometry lab at Box Hill within normal business hours Monday to Friday.

    Cut-off for outpatient sample collection on Fridays is at 11.30 AM and MUST reach the laboratory before 4.00PM.

    Flow cytometric analysis of cells stained by immunofluorescence with a range of antibodies against lymphoid and myeloid markers.

    The panel of markers used will be appropriate to the panel selected and the clinical comments supplied, or as arranged by prior consultation.

    Sample store at ROOM TEMPERATURE. Samples are not stable for more than 24 hours. Hence they must be received by the Flow Cytometry lab at Box Hill within normal business hours Monday to Friday.

    Cut-off for outpatient sample collection on Fridays is at 11.30 AM and MUST reach the laboratory before 4.00PM.

    Flow cytometric analysis of cells stained by immunofluorescence with a range of antibodies against lymphoid and myeloid markers.

    The panel of markers used will be appropriate to the panel selected and the clinical comments supplied, or as arranged by prior consultation.

    14th October 2021
    Collection & Request Instructions

    The samples are not stable for more than 24 hours. Hence all samples must be recieved in the laboratory at Box Hill  Monday to Friday within business hours.

    Cutoff for outpatient sample collection on Fridays is at 11.30 AM and MUST reach the laboratory before 4.00PM.

     

    Sample store at Room temperature. Samples are not stable for more than 24 hours. Hence they must be received by the Flow Cytometry lab at Box Hill within normal business hours Monday to Friday.

    Cut-off for outpatient sample collection on Fridays is at 11.30 AM and MUST reach the laboratory before 4.00PM.

    Flow cytometric analysis of cells stained by immunofluorescence with a range of antibodies against lymphoid and myeloid markers.

    The panel of markers used will be appropriate to the panel selected and the clinical comments supplied, or as arranged by prior consultation.

    Frequency

    Daily

    Notes

    Used to monitor T-cell numbers

    Preferred EDTA sample. Do not spin, store at room temperature.

    Samples must be marked 'URGENT', must reach the laboratory as soon as possible. 

    All out-of-hours testing requires notification.

    Time and date of collection must be recorded on request form.

    Whole blood - 1 x EDTA sample, do not spin. Store at room temperature. An FBE must also be requested, and needs a separate EDTA sample.

    14th October 2021
    Assay name

    T Cell Subsets

    Lymphocyte subsets

    Alternative names

    CD2, CD3, CD4, CD8

    T subsets include: CD3 count, CD4 count, CD8 count, CD4:CD8 ratio Lymphocyte subsets include: CD3 count, CD4 count, CD8 count, CD4:CD8 ratio, CD19 count & CD56 count

    Request Group

    FLOW

    TSUB & LSUB

    20th July 2021
    Processing Instructions

    Prefer EDTA sample.

    Do not spin, store at room temperature.

    Prefer EDTA sample.

    Do not spin, store at room temperature.

    20th July 2021
    Notes

    Used to monitor T-cell numbers

    Preferred EDTA sample. Do not spin, store at room temperature.

    Used to monitor T-cell numbers

    Preferred EDTA sample. Do not spin, store at room temperature.

    20th July 2021
    Collection & Request Instructions

    The samples are not stable for more than 24 hours. Hence all samples must be recieved in the laboratory at Box Hill  Monday to Friday within business hours.

    The samples are not stable for more than 24 hours. Hence all samples must be recieved in the laboratory at Box Hill  Monday to Friday within business hours.

    Cutoff for outpatient sample collection on Fridays is at 11.30 AM and MUST reach the laboratory before 4.00PM.

     

    Processing Instructions

    Prefer EDTA sample.

    Do not spin, store at room temperature.

    Prefer EDTA sample.

    Do not spin, store at room temperature.

    Notes

    Used to monitor T-cell numbers

    Preferred EDTA sample. Do not spin, store at room temperature.

    Used to monitor T-cell numbers

    Preferred EDTA sample. Do not spin, store at room temperature.


     Recent changes for Mycobacterium ulcerans PCR

    Recent changes for Mycobacterium ulcerans PCR

    Date
    Field
    Changed From
    Changed To
    3rd March 2021
    Alternative names

    Mycobacterium ulcerans NAD

    Mycobacterium ulcerans NAD, Buruli ulcer, Bairnsdale ulcer

    Collection & Request Instructions

     

    Specimen Required: Fresh tissue biopsy, dry swabs and paraffin-embedded fixed tissue sections (at least 6 sections, each 10-20 mm thick)

    Specimen Required: Fresh tissue biopsy, dry swabs (collect x2 - one for Mycobacterial culture and one for M.ulcerans PCR) and paraffin-embedded fixed tissue sections (at least 6 sections, each 10-20 mm thick). A gel swab may be used if unable to obtain a dry swab.


     Recent changes for Paroxysmal Nocturnal Haemoglobinuria

    Recent changes for Paroxysmal Nocturnal Haemoglobinuria

    Date
    Field
    Changed From
    Changed To
    15th October 2021
    Collection & Request Instructions

    Separate specimen required.

    Cutoff for outpatient sample collection on Fridays is at 11.30 AM and MUST reach the laboratory before 4.00PM.

    Whole blood - 1 x EDTA sample, do not spin. Store at room temperature. An FBE and BLOOD FILM must also be requested,  and needs a separate EDTA sample.

    Cutoff for outpatient sample collection on Fridays is at 11.30 AM and MUST reach the laboratory before 4.00PM.

    12th October 2021
    Alternative names

    CD 59, PNH flow cytometry, PNH screen

    CD59, PNH screen flow cytometry, FLEAR

    12th October 2021
    Alternative names

    CD 59, PNH screen

    CD 59, PNH screen, PNH flow cytometry

    Request Group

    FLOW

    PNHS

    20th July 2021
    Collection & Request Instructions

    Separate specimen required.

    Separate specimen required.

    Cutoff for outpatient sample collection on Fridays is at 11.30 AM and MUST reach the laboratory before 4.00PM.


     Recent changes for Plasminogen

    Recent changes for Plasminogen

    Date
    Field
    Changed From
    Changed To
    21st December 2020
    Volume (Adults)

    4 mL

    3.5 mL

    17th December 2020
    External Laboratory

    Austin Pathology

    Monash Pathology


     Recent changes for Procalcitonin

    Recent changes for Procalcitonin

    Date
    Field
    Changed From
    Changed To
    17th August 2022
    Method

    All PCT requests must be discussed with the Chemical Pathologist upon receipt in lab, regardless of time of day or day of week. If urgent/Taxi requiring is determined, result must be phoned to the clinician upon receipt - High Risk Result.

    27th July 2022
    Laboratory

    Biochemistry

    Referred Test

    Volume (Adults)

    0.5 mL

    External Laboratory

    Royal Childrens Hospital


     Recent changes for Protein C

    Recent changes for Protein C

    Date
    Field
    Changed From
    Changed To
    19th January 2021
    Collection & Request Instructions

    Transport specimen at Room Temperature to lab

    Collection & Request Instructions

    Fill to label marker and mix by inversion.

    Do not use 1 mL citrate tubes.

    1 x 3.5 ml citrate tubes sufficient for Protein C, Protein S and Antithrombin 3.

    Fill to label marker and mix by inversion.

    Do not use 1 mL citrate tubes.

    Samples must be transported to laboratory immediately. ( Within a maximun of 4 hours from collection time)

    1 x 3.5 ml citrate tubes sufficient for Protein C, Protein S and Antithrombin 3.

    10th December 2020
    Volume (Adults)

    3.5 mL

    10th December 2020
    Collection & Request Instructions

    Fill to label marker and mix by inversion.

    Do not use 1 mL citrate tubes.

    2 x 3.5 ml citrate tubes sufficient for Protein C, Protein S and Antithrombin 3.

    Fill to label marker and mix by inversion.

    Do not use 1 mL citrate tubes.

    1 x 3.5 ml citrate tubes sufficient for Protein C, Protein S and Antithrombin 3.

    Notes

    Results not valid if patient receiving warfarin therapy or with acute thrombotic event.

    Warfarin falsely lowers Protein C levels.

    Fill to label marker and mix by inversion.

    Do not use 1 mL citrate tubes.

    2 x 3.5 ml citrate sufficient for Protein C, Protein S and Antithrombin 3.

    Results not valid if patient receiving warfarin therapy or with acute thrombotic event.

    Warfarin falsely lowers Protein C levels.

    Fill to label marker and mix by inversion.

    Do not use 1 mL citrate tubes.

    1 x 3.5 ml citrate sufficient for Protein C, Protein S and Antithrombin 3.


     Recent changes for Protein S

    Recent changes for Protein S

    Date
    Field
    Changed From
    Changed To
    19th January 2021
    Collection & Request Instructions

    Fill to label marker and mix by inversion

    Do not use 1 ml citrate tubes.

    1 x 3.5 mL citrate tubes sufficient for Protein C, Protein S and Antithrombin 3.

    Fill to label marker and mix by inversion

    Do not use 1 ml citrate tubes.

    Samples must be transported to laboratory immediately. ( Within a maximun of 4 hours from collection time)

    1 x 3.5 mL citrate tubes sufficient for Protein C, Protein S and Antithrombin 3.

    10th December 2020
    Volume (Adults)

    7 mL

    3.5 mL

    10th December 2020
    Collection & Request Instructions

    Fill to label marker and mix by inversion

    Do not use 1 ml citrate tubes.

    2 x 3.5 mL citrate tubes sufficient for Protein C, Protein S and Antithrombin 3.

    Fill to label marker and mix by inversion

    Do not use 1 ml citrate tubes.

    1 x 3.5 mL citrate tubes sufficient for Protein C, Protein S and Antithrombin 3.

    Notes

    Results not valid if patient receiving warfarin therapy or with acute thrombotic event

    Warfarin falsely lowers Protein S level.

    Fill to label marker and mix by inversion

    Do not use 1 ml citrate tubes.

    2 x 3.5 mL citrate tubes sufficient for Protein C, Protein S and Antithrombin 3.

    Results not valid if patient receiving warfarin therapy or with acute thrombotic event

    Warfarin falsely lowers Protein S level.

    Fill to label marker and mix by inversion

    Do not use 1 ml citrate tubes.

    1 x 3.5 mL citrate tubes sufficient for Protein C, Protein S and Antithrombin 3.


     Recent changes for Prothrombin Time

    Recent changes for Prothrombin Time

    Date
    Field
    Changed From
    Changed To
    25th March 2022
    Collection & Request Instructions

    Filled to label marker and mixed by inversion.

    Patient Information - Warfarin Management

     

    Filled to label marker and mixed by inversion.

    Patient Information - Warfarin Management-English
    Patient Information - Warfarin Management-Greek
    Patient Information - Warfarin Management-Simplified Chinese

     

     


     Recent changes for Red Cell Antigen Genotyping

    Recent changes for Red Cell Antigen Genotyping

    Date
    Field
    Changed From
    Changed To
    9th May 2022
    Collection & Request Instructions

    Minimum volume of 4 ml required - this specimen cannot be used for any other purpose.

    A completed "ARCBS Red Cell Reference Form" must accompany each sample sent for analysis.

    This form, which can be completed online and subsequently printed to be sent with the sample can be found by clicking here 

    Minimum volume of 4 ml required - this specimen cannot be used for any other purpose.

    A completed "ARCBS Red Cell Reference Form" must accompany each sample sent for analysis.

    This form, which can be completed online and subsequently printed to be sent with the sample can be found by clicking here 

    Processing Instructions

    Specimen will arrive in department at RT.  Please keep at 4 degrees prior to transport.

    Specimen will arrive in department at RT.  Please keep at 4 degrees prior to transport.

    External Transport Instructions

    Transport at 4 degrees

    Transport at 4 degrees


     Recent changes for Risperidone

    Recent changes for Risperidone

    Date
    Field
    Changed From
    Changed To
    18th December 2020
    External Laboratory

    Pathwest Lab Med WA

    Alfred Hospital


     Recent changes for Ristocetin Co-Factor assay

    Recent changes for Ristocetin Co-Factor assay

    Date
    Field
    Changed From
    Changed To
    11th June 2021
    Alternative names

    RICOF

    RICOF, vWF:Activity, vWF:Act

    Laboratory

    Referred Test

    Coagulation

    External Laboratory

    Austin Pathology


     Recent changes for Scabies investigation

    Recent changes for Scabies investigation

    Date
    Field
    Changed From
    Changed To
    8th June 2021
    Collection & Request Instructions

    Scabies collection instructions

    Preparation

    Wash the area of skin to be scraped with an alcohol swab.

    Remove any ointment or residual applications using soap and water.

    Disinfect the area with a skin cleansing alcohol wipe and allow it to dry.

    Abrade the infested area with a sterile scalpel to the depth of pinpoint bleeding.

    Transfer skin scrapings to a sterile yellow top pot for transport to the laboratory. Do NOT include the scalpel – dispose of in sharps bin

    Label pot with patient name, UR No and DOB, date of collection, site of collection

    Preparation

    Wash the area of skin to be scraped with an alcohol swab.

    Remove any ointment or residual applications using soap and water.

    Disinfect the area with a skin cleansing alcohol wipe and allow it to dry.

    Abrade the infested area with a sterile scalpel to the depth of pinpoint bleeding.

    Transfer skin scrapings to a sterile yellow top pot for transport to the laboratory. Do NOT include the scalpel – dispose of in sharps bin

    Label pot with patient name, UR No and DOB, date of collection, site of collection


     Recent changes for Test

    Recent changes for Test

    Date
    Field
    Changed From
    Changed To
    3rd December 2020
    Method

    Laboratory notes testing

    Laboratory notes - email test


     Recent changes for Thrombophilia Screen

    Recent changes for Thrombophilia Screen

    Date
    Field
    Changed From
    Changed To
    10th December 2020
    Collection & Request Instructions

    For full thrombophilia screen including INR, APTT, Protein C, Protein S, AT3, LA screen, APCR, Homocystine, Factor V Leiden, PG20210A and ACA:-

    5 x 3.5 ml citrate tubes plus 2 EDTA tubes and 1 x serum tube will be required.

    For full thrombophilia screen including INR, APTT, Protein C, Protein S, AT3, LA screen, , Homocystine, Factor V Leiden, PG20210A and ACA/ACL antibodies:-

    3 x 3.5 ml citrate tubes plus 2 EDTA tubes and 1 x serum tube will be required.

    Notes

    May include Protein C functional, Protein S free, Antithrombin III, APCR ratio, Factor V Leiden, Homocystine, Anti-cardiolipin antibodies, Lupus Anticoagulant, Prothrombin mutation.

    Which tests are most appropriate depends on clinical history

    Tests need to be listed individually to comply with HIC guidelines

    Consult Haematology Registrar or Haematologist.

    For full thrombophilia screen including INR, APTT, Protein C, Protein S, AT3, LA screen, APCR, Homocystine, Factor V Leiden, PG20210A and ACA:- 5 x 3.5 ml citrate tubes plus 2 EDTA tubes and 1 x serum tube will be required.

    May include Protein C functional, Protein S free, Antithrombin III,  Factor V Leiden, Homocystine, Anti-cardiolipin antibodies, Lupus Anticoagulant, Prothrombin Gene Mutation.

    Which tests are most appropriate depends on clinical history

    Tests need to be listed individually to comply with HIC guidelines

    Consult Haematology Registrar or Haematologist.

    For full thrombophilia screen including INR, APTT, Protein C, Protein S, AT3, LA screen, Homocystine, Factor V Leiden, PG20210A and ACA:- 3 x 3.5 ml citrate tubes plus 2 EDTA tubes and 1 x serum tube will be required.


     Recent changes for Tissue release from Anatomical Pathology

    Recent changes for Tissue release from Anatomical Pathology

    Date
    Field
    Changed From
    Changed To
    6th March 2022
    Laboratory

    Unassigned

    Anatomical Pathology

    6th March 2022
    External Transport Instructions

    https://pathology.easternhealth.org.au/handbook/downloads/anatomical-pathology/Tissue%20Release%20Consent%20Form.doc


     Recent changes for Vitamin C

    Recent changes for Vitamin C

    Date
    Field
    Changed From
    Changed To
    31st March 2022
    Volume (Adults)

    2 mL

    1 mL

    Minimum/Paediatric Volume

    1 mL

    Processing Instructions

    Spin, separate, wrap in foil and freeze ASAP.

    Transport frozen. Contact Gribbles to arrange courier.

    Spin, separate, wrap in foil and freeze ASAP.

    Transport frozen. 

    External Laboratory

    Australian Clinical Labs (formerly Healthscope)

    Alfred Hospital

    Frequency

    Weekly

    Fortnightly


     Recent changes for Xanthochromia

    Recent changes for Xanthochromia

    Date
    Field
    Changed From
    Changed To
    14th July 2021
    Laboratory

    Referred Test

    Biochemistry

    Collection & Request Instructions

    Please note : to avoid sample deterioration, DO NOT send CSF specimens via the vacuum chute system.

    Please quickly check that the tube is labelled underneath the foil before referring.

    THERE MUST BE A MINIMUM OF 0.6 mL OF CSF OR THE TEST CANNOT BE PERFORMED

     

    Please note : to avoid sample deterioration, DO NOT send CSF specimens via the vacuum chute system.

     

    THERE MUST BE A MINIMUM OF 0.6 mL OF CSF OR THE TEST CANNOT BE PERFORMED

     

    Processing Instructions

    CSF xanthochromia checklist

    Sample collected >12hrs from onset symptoms   Preferable

     

          4th CSF sample    Essential

     

    Sample light protected immediately   Essential

     

    Sample transported manually to lab   Essential

     

    Serum sample for bilirubin & albumin   Preferable

     

    Slip with clinical notes and contact details   Essential

     

    CSF xanthochromia checklist

    Sample collected >12hrs from onset symptoms   Preferable

     

          4th CSF sample    Essential

     

    Sample light protected immediately   Essential

     

    Sample transported manually to lab   Essential

     

    Serum sample for bilirubin & protein- essemtial

     

    Slip with clinical notes and contact details   Essential

     

    External Laboratory

    Austin Pathology

    External Transport Instructions

    Send via a Taxi ASAP. Ring Austin Pathology on 94963100.

    MH and AH send via taxi ASAP and notify BH laboraotry.

    Frequency

    Daily

    As required

    Method

    Please quickly check that the tube is labelled underneath the foil before passing to Scientist.

    The specimen designated for spectrophotometry should be centrifuged at .2000 rpm for 5 min as soon as possible after receipt in the laboratory. The supernatant must be stored in the dark at 40 C until analysis.

    Notes

    This test is specialised and will only be performed on CSF's when:

    CT scan is negative

     

    Four tubes must be collected and the container of the 4th tube must be wrapped in foil (please Label specimen before wrapping in foil)

    This test is specialised and will only be performed on CSF's when:

    CT scan is negative

    Four tubes must be collected and the container of the 4th tube must be wrapped in foil (please Label specimen before wrapping in foil)

    A white top tube must be collected for simulataneous measurement of serum Bilirubin and serum Protein (or add on to existing bloods within 6 hours of CSF collection).