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Recent Changes - Test & Collection Guide

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 Recent changes for Alpha Foetoprotein

Recent changes for Alpha Foetoprotein

Date
Field
Changed From
Changed To
17th January 2020
Collection & Request Instructions

Clinical notes must be completed

If pregnant female, please state pregancy in weeks

AFP is useful in monitoring some testicular germ cell tumours and primary hepatocelluar carcnioma. After treatment, eg. orchidectomy, levels fall with a half-life of approximately 45 days. Values > 500 mg/L are suggestive of malignancy

However 50% of all malignancies have values < 500 mg/L. AFP can also be raised in a variety of non-malignant liver disorders, where levels are usually < 100 mg/L.

Clinical notes must be completed

If pregnant female, please state pregancy in weeks

AFP is useful in monitoring some testicular germ cell tumours and primary hepatocelluar carcnioma. After treatment, eg. orchidectomy, levels fall with a half-life of approximately 45 days. Values > 500 ug/L are suggestive of malignancy

However 50% of all malignancies have values < 500 ug/L. AFP can also be raised in a variety of non-malignant liver disorders, where levels are usually < 100 ug/L.


 Recent changes for Aluminium

Recent changes for Aluminium

Date
Field
Changed From
Changed To
12th February 2020
Collection & Request Instructions

 

LAB STAFF NOTE : The Reference Lab will only receive aliquots which are sent in a metal-free aliquot tube (see container picture above).  Sample must be decanted by pour-off (do not use plastic pipettes). 

 

LAB STAFF NOTE : The Reference Lab will only receive aliquots which are sent in a metal-free aliquot tube (see container picture above).  Sample must be decanted by pour-off (do not use plastic pipettes). 

7th February 2020
Collection & Request Instructions

The Reference Lab will only receive aliquots which are sent in a metal-free aliquot tube (see container picture above).  Sample must be decanted by pour-off (do not use plastic pipettes). 

 

LAB STAFF NOTE : The Reference Lab will only receive aliquots which are sent in a metal-free aliquot tube (see container picture above).  Sample must be decanted by pour-off (do not use plastic pipettes). 

7th February 2020
Preferred Container Type

Trace metal tube ( Dark blue )

Trace Metal with Aliquot

Collection & Request Instructions

Do Not spin - Transport at 40C

The Reference Lab will only receive aliquots which are sent in a metal-free aliquot tube (see container picture above).  Sample must be decanted by pour-off (do not use plastic pipettes). 


 Recent changes for Anti-diuretic hormone

Recent changes for Anti-diuretic hormone

Date
Field
Changed From
Changed To
7th October 2021
Alternative names

ADH

Request Group

ADH

Preferred Specimen Type

Plasma

Serum

Optional Specimen Type

Plasma

Preferred Container Type

EDTA

Serum tube with gel

Optional Container Type

EDTA
Lithium heparin no gel

Collection & Request Instructions

Samples should be fasting.

Processing Instructions

Spin, separate and freeze plasma.

Packed by certified personnel.

Sent frozen.

Specimen Reception, Level 6 (E Block), RPA, Missenden Road, Camperdown , NSW 2050

Tel: 02-95158279

Spin, separate and freeze serum/plasma

Packed by certified personnel.

Sent frozen.

Specimen Reception, Level 6 (E Block), RPA, Missenden Road, Camperdown , NSW 2050

Tel: 02-95158279


 Recent changes for Anti-Thrombin III

Recent changes for Anti-Thrombin III

Date
Field
Changed From
Changed To
19th January 2021
Collection & Request Instructions

Fill to label marker and mix by inversion.

DO NOT use 1 mL citrate tubes.

1 x 3.5 ml citrate tubes sufficient for Protein C, Protein S and Antithrombin 3.

Fill to label marker and mix by inversion.

DO NOT use 1 mL citrate tubes.

Samples must be transported to laboratory immediately. ( Within a maximun of 4 hours from collection time)

1 x 3.5 ml citrate tubes sufficient for Protein C, Protein S and Antithrombin 3.

10th December 2020
Collection & Request Instructions

Fill to label marker and mix by inversion.

DO NOT use 1 mL citrate tubes.

Fill to label marker and mix by inversion.

DO NOT use 1 mL citrate tubes.

1 x 3.5 ml citrate tubes sufficient for Protein C, Protein S and Antithrombin 3.


 Recent changes for Anti-Xa Assay

Recent changes for Anti-Xa Assay

Date
Field
Changed From
Changed To
18th May 2021
Request Group

10A: Clexane, 10AH:Plasma Heparin, APIX: Apixaban, RIVX: Rivaroxaban

10A: Clexane, 10AH:Plasma Heparin, APIX: Apixaban, RIVX: Rivaroxaban, FONX: Fondaparinux

18th May 2021
Request Group

10A

10A: Clexane, 10AH:Plasma Heparin, APIX: Apixaban, RIVX: Rivaroxaban


 Recent changes for Beta - 2-Transferrin, Asialo - Fluid

Recent changes for Beta - 2-Transferrin, Asialo - Fluid

Date
Field
Changed From
Changed To
19th May 2020
Volume (Adults)

1.0 mL

Minimum/Paediatric Volume

0.2 mL

Collection & Request Instructions

Wicks and Gauze will not be accepted.

Notes

The presence of asialotransferrin in a fluid indicates that it is, or contains, CSF.

The presence of asialotransferrin in a fluid indicates that it is, or contains, CSF.

Pathologist approval required.


 Recent changes for Blood Cultures - Adult

Recent changes for Blood Cultures - Adult

Date
Field
Changed From
Changed To
9th August 2021
Processing Instructions

Bottles should be loaded on to the Bactec analyser as soon as possible. If entry is delayed, store bottles at room temperature.

Bottles should be loaded on to the Bactec FX analyser as soon as possible. If entry is delayed, store bottles at room temperature.

9th August 2021
Processing Instructions

Bottles should be loaded on to the Virtuo analyser as soon as possible. If entry is delayed, store bottles at room temperature.

Bottles should be loaded on to the Bactec analyser as soon as possible. If entry is delayed, store bottles at room temperature.

25th May 2021
Volume (Adults)

10 mL

8 mL

Preferred Container Type

Blood Culture

Blood Culture (Adult)


 Recent changes for Blood Cultures - Children and Neonates

Recent changes for Blood Cultures - Children and Neonates

Date
Field
Changed From
Changed To
9th August 2021
External Transport Instructions

Load as soon as possible onto the Virtuo Analyzer. Store at room temperature until loaded

Load as soon as possible onto the Bactec FX Analyzer. Store at room temperature until loaded

25th May 2021
Volume (Adults)

4 mL

Minimum/Paediatric Volume

1 mL

Preferred Container Type

Blood Culture Paediatric

Blood Culture (Paediatric)

Collection & Request Instructions

Add 0.5 - 4mL of blood to a single aerobic paediatric bottle.

For patients with suspected bacterial endocarditis, 2 to 3 sets taken at different times should be sufficient to establish a diagnosis.

Collect blood cultures 20 minutes apart. If patient is too ill to wait 20 minutes, prior to administering antibiotics, collect the first set from one site and the second from a different site.

Please provide clinical details. Extended incubation is required for query endocarditis, brucellosis or fungal infection

Transport at room temperature

 

Add 1 - 3mL of blood to a single aerobic paediatric bottle.

For patients with suspected bacterial endocarditis, 2 to 3 sets taken at different times should be sufficient to establish a diagnosis.

If multiple sets required, collect blood cultures 20 minutes apart. If patient is too ill to wait 20 minutes, prior to administering antibiotics, collect the first set from one site and the second from a different site.

Please provide clinical details. Extended incubation is required for query endocarditis, brucellosis or fungal infection

Transport at room temperature

 


 Recent changes for Bone Specific Alkaline Phosphatase

Recent changes for Bone Specific Alkaline Phosphatase

Date
Field
Changed From
Changed To
15th June 2021
Assay name

Bone Specific Alkiline Phosphatase

Bone Specific Alkaline Phosphatase

External Laboratory

Monash Pathology

Royal Melbourne Hospital


 Recent changes for BRCA1/BRCA2

Recent changes for BRCA1/BRCA2

Date
Field
Changed From
Changed To
17th June 2021
Request Group

CHROM

Collection & Request Instructions

Send to Peter Mac on next available courier. Stable at room temperature for a maximum of 2 weeks.

Processing Instructions

Send to Peter Mac on next available courier. Stable at room temperature for a maximum of 2 weeks.

External Transport Instructions

Room Temperature

Room Temperature

Send to Peter Mac on next available courier. Stable at room temperature for a maximum of 2 weeks.

Method

Send to Peter Mac on next available courier. Stable at room temperature for a maximum of 2 weeks.


 Recent changes for CADASIL-Notch 3 gene mutation

Recent changes for CADASIL-Notch 3 gene mutation

Date
Field
Changed From
Changed To
7th November 2019
External Laboratory

Neurogenetics Unit, Level 2, Royal Perth Hospital

Pathwest Lab Med WA

Frequency

As required

Method

Packed by certified personnel

Dr M Davis, Neurogenetics Unit, DPT of Anatomical Pathology, Level 2, North Block, RPA, Wellington Street, PERTH, WA 6000

(Ph) 08-92243136.

Packed by certified personnel

Dr M Davis, Diagnostic Genomics, Level 2

C/O Specimen Reception Ground Floor PP Building

QEII Medical Centre Hospital Avenue

Nedlands WA 6009 AUSTRALIA

(Ph) 08-63834243

Notes

Defects in NOTCH3 are the cause of cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL) . CADASIL causes a type of stroke and dementia of which key features include recurrent subcortical ischemic events and vascular dementia. The disorder affects relatively young adults of both sexes. Mutations affect highly conserved cysteine residues within epidermal growth factor (EGF)-like repeat domains in the extracellular part of the receptor.

Defects in NOTCH3 are the cause of cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL) . CADASIL causes a type of stroke and dementia of which key features include recurrent subcortical ischemic events and vascular dementia. The disorder affects relatively young adults of both sexes. Mutations affect highly conserved cysteine residues within epidermal growth factor (EGF)-like repeat domains in the extracellular part of the receptor.


 Recent changes for Cadmium

Recent changes for Cadmium

Date
Field
Changed From
Changed To
12th February 2020
Preferred Container Type

Trace Metal with Aliquot

Trace metal tube ( Dark blue )

Notes

Transport specimen at Room Temperature to lab
Sample haemolysis may make this test unreportable

 

LAB STAFF NOTE : The Reference Lab will only receive aliquots which are sent in a metal-free aliquot tube (see container picture above).  Sample must be decanted by pour-off (do not use plastic pipettes). 

Transport specimen at Room Temperature to lab
Sample haemolysis may make this test unreportable

 

 


 Recent changes for Candida auris screen

Recent changes for Candida auris screen

Date
Field
Changed From
Changed To
15th November 2019
Alternative names

Candida screen

Candida screen, Candida auris


 Recent changes for Cerebrospinal Fluid - Biochemistry

Recent changes for Cerebrospinal Fluid - Biochemistry

Date
Field
Changed From
Changed To
27th April 2021
Collection & Request Instructions

Please note : to avoid sample deterioration, DO NOT send CSF specimens via the vacuum chute system.

Depending on the tests requested, 3-4 separate tubes will need to be provided.  Note that the tubes are numbered so that order of collection can be identified – this order should be carefully followed, so that the optimal sample can be used for the requested tests.  It is also important to consider the tests that are required, as this will determine how much CSF is needed in each tube.  See the matrix below for details.

TUBE 1 is used for Cytology.

TUBE 2 is used for Glucose, Protein and Flow Cytometry.

(centrifuged in the lab – the supernatant is used for Biochemistry analysis (glucose, protein) and the cellular pellet will be used for flow cytometry if required).

TUBE 3 is for Microbiological analysis (cell count, Gram stain and culture) and any further infectious testing (e.g. viral PCR, mycobacterial PCR and culture, cryptococcal antigen).

TUBE 4 will be required only for;

         (a) Creutzfeld-Jacob Disease (CJD) testing OR

(b) Xanthochromia analysis (wrap the tube in foil to prevent    sample deterioration due to light).

 

TESTS REQUIRED

MINIMUM REQUIRED VOLUME (mL)

TUBE 1

TUBE 2

TUBE 3

TUBE 4

CYTOLOGY. CHEMISTRY, MICRO/CULTURE

1.0-2.0

1.0

1.0-2.0

Nil

ADD FLOW CYTOMETRY

 

add 5.0-7.0

 

 

ADD OTHER INFECTIOUS MARKERS

 

 

add 2.0- 5.0-

 

ADD MYCOBACTERIAL CULTURE

 

 

Add 10.00

 

ADD CJD

 

 

 

1.0

ADD XANTHOCHROMIA

 

 

 

1.0 (foil)

 

Note, the ability to add further tests to a previously collected sample is dependent upon the volume provided.  Also, many tests (flow cytometry, cytology) can only be performed on fresh specimens.

 

Other specialised testing (e.g. oligoclonal bands) will be performed using the supplied samples.

 

Xanthochromia and CJD analysis cannot be performed on a blood-stained sample.

CSF Information - Laboratory Processing

Depending on the tests requested, 3-4 separate tubes will need to be provided. Note that the tubes are numbered so that order of collection can be identified – this order should be carefully followed, so that the optimal sample can be used for the requested tests. It is also important to consider the tests that are required, as this will determine how much CSF is needed in each tube. See the matrix below for details.

TUBE 1 is used for Biochemistry and Flow Cytometry. (Centrifuged in the lab – the supernatant is used for Biochemistry analysis (glucose, protein) and the cellular pellet will be used for flow cytometry if required). If tube 3 is bloodstained, take an aliquot for microbiology.

TUBE 2 is used for Cytology

TUBE 3 is for Microbiological analysis (cell count, Gram stain and culture) and any further infectious testing (e.g. viral PCR, mycobacterial PCR and culture, cryptococcal antigen).

TUBE 4 will be required only for;

(a) Creutzfeld-Jacob Disease (CJD) testing OR

(b) Xanthochromia analysis (wrap the tube in foil to prevent sample deterioration due to light).

 

TESTS REQUIRED

MINIMUM REQUIRED VOLUME (mL)

TUBE 1

TUBE 2

TUBE 3

TUBE 4

CHEMISTRY

1.0

     

ADD FLOW CYTOMETRY

Minimum 5.0-7.0

     

CYTOLOGY

 

1.0-3.0

   

MICROBIOLOGY

   

1.0-2.0

 

ADD OTHER INFECTIOUS MARKERS

   

add 2.0- 5.0-

 

ADD MYCOBACTERIAL CULTURE

   

Add 6.0-10.00

 

ADD CJD

     

1.0

ADD XANTHOCHROMIA

     

1.0 (foil)

 

Note, the ability to add further tests to a previously collected sample is dependent upon the volume provided. Also, many tests (flow cytometry, cytology) can only be performed on fresh specimens.

 

Other specialised testing (e.g. oligoclonal bands) will be performed using the supplied samples.

Xanthochromia and CJD analysis cannot be performed on a blood-stained sample.

 

14th August 2020
Collection & Request Instructions

Please note : to avoid sample deterioration, DO NOT send CSF specimens via the vacuum chute system.

Depending on the tests requested, 3-4 separate tubes will need to be provided.  Note that the tubes are numbered so that order of collection can be identified – this order should be carefully followed, so that the optimal sample can be used for the requested tests.  It is also important to consider the tests that are required, as this will determine how much CSF is needed in each tube.  See the matrix below for details.

TUBE 1 is used for Cytology.

TUBE 2 is used for Glucose, Protein and Flow Cytometry.

(centrifuged in the lab – the supernatant is used for Biochemistry analysis (glucose, protein) and the cellular pellet will be used for flow cytometry if required).

TUBE 3 is for Microbiological analysis (cell count, Gram stain and culture) and any further infectious testing (e.g. viral PCR, mycobacterial PCR and culture, cryptococcal antigen).

TUBE 4 will be required only for;

         (a) Creutzfeld-Jacob Disease (CJD) testing OR

(b) Xanthochromia analysis (wrap the tube in foil to prevent    sample deterioration due to light).

 

TESTS REQUIRED

MINIMUM REQUIRED VOLUME (mL)

TUBE 1

TUBE 2

TUBE 3

TUBE 4

CYTOLOGY. CHEMISTRY, MICRO/CULTURE

1.0-2.0

1.0

1.0-2.0

Nil

ADD FLOW CYTOMETRY

 

add 5.0-7.0

 

 

ADD OTHER INFECTIOUS MARKERS

 

 

add 2.0- 5.0-

 

ADD MYCOBACTERIAL CULTURE

 

 

Add 10.00

 

ADD CJD

 

 

 

1.0

ADD XANTHOCHROMIA

 

 

 

1.0 (foil)

 

Note, the ability to add further tests to a previously collected sample is dependent upon the volume provided.  Also, many tests (flow cytometry, cytology) can only be performed on fresh specimens.

 

Other specialised testing (e.g. oligoclonal bands) will be performed using the supplied samples.

 

Xanthochromia and CJD analysis cannot be performed on a blood-stained sample.

Please note : to avoid sample deterioration, DO NOT send CSF specimens via the vacuum chute system.

Depending on the tests requested, 3-4 separate tubes will need to be provided.  Note that the tubes are numbered so that order of collection can be identified – this order should be carefully followed, so that the optimal sample can be used for the requested tests.  It is also important to consider the tests that are required, as this will determine how much CSF is needed in each tube.  See the matrix below for details.

TUBE 1 is used for Cytology.

TUBE 2 is used for Glucose, Protein and Flow Cytometry.

(centrifuged in the lab – the supernatant is used for Biochemistry analysis (glucose, protein) and the cellular pellet will be used for flow cytometry if required).

TUBE 3 is for Microbiological analysis (cell count, Gram stain and culture) and any further infectious testing (e.g. viral PCR, mycobacterial PCR and culture, cryptococcal antigen).

TUBE 4 will be required only for;

         (a) Creutzfeld-Jacob Disease (CJD) testing OR

(b) Xanthochromia analysis (wrap the tube in foil to prevent    sample deterioration due to light).

 

TESTS REQUIRED

MINIMUM REQUIRED VOLUME (mL)

TUBE 1

TUBE 2

TUBE 3

TUBE 4

CYTOLOGY. CHEMISTRY, MICRO/CULTURE

1.0-2.0

1.0

1.0-2.0

Nil

ADD FLOW CYTOMETRY

 

add 5.0-7.0

 

 

ADD OTHER INFECTIOUS MARKERS

 

 

add 2.0- 5.0-

 

ADD MYCOBACTERIAL CULTURE

 

 

Add 10.00

 

ADD CJD

 

 

 

1.0

ADD XANTHOCHROMIA

 

 

 

1.0 (foil)

 

Note, the ability to add further tests to a previously collected sample is dependent upon the volume provided.  Also, many tests (flow cytometry, cytology) can only be performed on fresh specimens.

 

Other specialised testing (e.g. oligoclonal bands) will be performed using the supplied samples.

 

Xanthochromia and CJD analysis cannot be performed on a blood-stained sample.

CSF Information - Laboratory Processing

14th August 2020
Collection & Request Instructions

Please note : to avoid sample deterioration, DO NOT send CSF specimens via the vacuum chute system.

If additional tests required eg. CSF oligoclonal bands, a minimum of 1.0 ml is required.

Collect CSF into three separate, sterile, screw-capped tubes provided (labelled 1,2 & 3)

Tube 1 is for Biochemistry (for Protein and Glucose) 0.5ml (10 drops) required 

Tube 2 for Cytology 1.0ml (20 drops)  and/or Flow Cytometry (6ml)

For Flow Cytometry a minimum of 6ml is required  in Tube 2

For Flow Cytometry and Cytology a minimum of 7ml is required in Tube 2     

Tube 3 for Microbiology upto 10ml (Full tube)

If querying CJD the laboratory requires a 4th unopened tube containing at least 1ml of non-bloodstained CSF.

Xanthochromia investigation requires a 4th tube containing 1ml of CSF .

Please note : to avoid sample deterioration, DO NOT send CSF specimens via the vacuum chute system.

Depending on the tests requested, 3-4 separate tubes will need to be provided.  Note that the tubes are numbered so that order of collection can be identified – this order should be carefully followed, so that the optimal sample can be used for the requested tests.  It is also important to consider the tests that are required, as this will determine how much CSF is needed in each tube.  See the matrix below for details.

TUBE 1 is used for Cytology.

TUBE 2 is used for Glucose, Protein and Flow Cytometry.

(centrifuged in the lab – the supernatant is used for Biochemistry analysis (glucose, protein) and the cellular pellet will be used for flow cytometry if required).

TUBE 3 is for Microbiological analysis (cell count, Gram stain and culture) and any further infectious testing (e.g. viral PCR, mycobacterial PCR and culture, cryptococcal antigen).

TUBE 4 will be required only for;

         (a) Creutzfeld-Jacob Disease (CJD) testing OR

(b) Xanthochromia analysis (wrap the tube in foil to prevent    sample deterioration due to light).

 

TESTS REQUIRED

MINIMUM REQUIRED VOLUME (mL)

TUBE 1

TUBE 2

TUBE 3

TUBE 4

CYTOLOGY. CHEMISTRY, MICRO/CULTURE

1.0-2.0

1.0

1.0-2.0

Nil

ADD FLOW CYTOMETRY

 

add 5.0-7.0

 

 

ADD OTHER INFECTIOUS MARKERS

 

 

add 2.0- 5.0-

 

ADD MYCOBACTERIAL CULTURE

 

 

Add 10.00

 

ADD CJD

 

 

 

1.0

ADD XANTHOCHROMIA

 

 

 

1.0 (foil)

 

Note, the ability to add further tests to a previously collected sample is dependent upon the volume provided.  Also, many tests (flow cytometry, cytology) can only be performed on fresh specimens.

 

Other specialised testing (e.g. oligoclonal bands) will be performed using the supplied samples.

 

Xanthochromia and CJD analysis cannot be performed on a blood-stained sample.


 Recent changes for Cerebrospinal Fluid - Cytology

Recent changes for Cerebrospinal Fluid - Cytology

Date
Field
Changed From
Changed To
27th April 2021
Collection & Request Instructions

The sample with the least amount of blood is prefered for Cytology.

CSF SPECIMEN INFORMATION – LABORATORY PROCESSING

Depending on the tests requested, 3-4 separate tubes will need to be provided.  Note that the tubes are numbered so that order of collection can be identified – this order should be carefully followed, so that the optimal sample can be used for the requested tests.  It is also important to consider the tests that are required, as this will determine how much CSF is needed in each tube.  See the matrix below for details.

TUBE 1 is used for Cytology.

TUBE 2 is used for Glucose, Protein and Flow Cytometry.

(centrifuged in the lab – the supernatant is used for Biochemistry analysis (glucose, protein) and the cellular pellet will be used for flow cytometry if required).

TUBE 3 is for Microbiological analysis (cell count, Gram stain and culture) and any further infectious testing (e.g. viral PCR, mycobacterial PCR and culture, cryptococcal antigen).

TUBE 4 will be required only for;

         (a) Creutzfeld-Jacob Disease (CJD) testing OR

(b) Xanthochromia analysis (wrap the tube in foil to prevent    sample deterioration due to light).

 

TESTS REQUIRED

MINIMUM REQUIRED VOLUME (mL)

TUBE 1

TUBE 2

TUBE 3

TUBE 4

CYTOLOGY. CHEMISTRY, MICRO/CULTURE

1.0-2.0

1.0

1.0-2.0

Nil

ADD FLOW CYTOMETRY

 

add 5.0-7.0

 

 

ADD OTHER INFECTIOUS MARKERS

 

 

add 2.0- 5.0-

 

ADD MYCOBACTERIAL CULTURE

 

 

Add 10.00

 

ADD CJD

 

 

 

1.0

ADD XANTHOCHROMIA

 

 

 

1.0 (foil)

 

Note, the ability to add further tests to a previously collected sample is dependent upon the volume provided.  Also, many tests (flow cytometry, cytology) can only be performed on fresh specimens.

 

Other specialised testing (e.g. oligoclonal bands) will be performed using the supplied samples.

 

Xanthochromia and CJD analysis cannot be performed on a blood-stained sample.

The sample with the least amount of blood is prefered for Cytology.

CSF SPECIMEN INFORMATION – LABORATORY PROCESSING

Depending on the tests requested, 3-4 separate tubes will need to be provided.  Note that the tubes are numbered so that order of collection can be identified – this order should be carefully followed, so that the optimal sample can be used for the requested tests.  It is also important to consider the tests that are required, as this will determine how much CSF is needed in each tube.  See the matrix below for details.

TUBE 1 is used for Biochemistry and Flow Cytometry. (Centrifuged in the lab – the supernatant is used for Biochemistry analysis (glucose, protein) and the cellular pellet will be used for flow cytometry if required). If tube 3 is bloodstained, take an aliquot for microbiology.

TUBE 2 is used for Cytology

TUBE 3 is for Microbiological analysis (cell count, Gram stain and culture) and any further infectious testing (e.g. viral PCR, mycobacterial PCR and culture, cryptococcal antigen).

TUBE 4 will be required only for;

(a) Creutzfeld-Jacob Disease (CJD) testing OR

(b) Xanthochromia analysis (wrap the tube in foil to prevent sample deterioration due to light).

 

TESTS REQUIRED

MINIMUM REQUIRED VOLUME (mL)

TUBE 1

TUBE 2

TUBE 3

TUBE 4

CHEMISTRY

1.0

     

ADD FLOW CYTOMETRY

Minimum 5.0-7.0

     

CYTOLOGY

 

1.0-3.0

   

MICROBIOLOGY

   

1.0-2.0

 

ADD OTHER INFECTIOUS MARKERS

   

add 2.0- 5.0-

 

ADD MYCOBACTERIAL CULTURE

   

Add 6.0-10.00

 

ADD CJD

     

1.0

ADD XANTHOCHROMIA

     

1.0 (foil)

 

Note, the ability to add further tests to a previously collected sample is dependent upon the volume provided. Also, many tests (flow cytometry, cytology) can only be performed on fresh specimens.

 

Other specialised testing (e.g. oligoclonal bands) will be performed using the supplied samples.

Xanthochromia and CJD analysis cannot be performed on a blood-stained sample.

 

 

Method

CSF sample processing:

If Cytology/Flow Cytometry requested  : Tube 1-Biochemistry, Tube 2- Cytology/Flow Cytometry, Tube 3-Microbiology

If Cytology/Flow Cytometry not requested: Tube 1 and 3-Microbiology, Tube 2-Biochemistry

Any confusion between the terms cell count and cytology could be cleared up when the cell count is rung through to the requesting doctor by the scientist. At this point it could be determined whether cytology is a genuine request or not (all to be documented in F8 notes).

 

 

14th August 2020
Collection & Request Instructions

The sample with the least amount of blood is prefered for Cytology.

Collect CSF into three separate, sterile, screw-capped tubes provided (labelled 1,2 & 3)

Tube 1 is for Biochemistry (for Protein and Glucose) 0.5ml (10 drops) required

Tube 2 for Cytology 1.0ml (20 drops)  and/or Flow Cytometry (6ml)

For Flow Cytometry a minimum of 6ml is required  in Tube 2

For Flow Cytometry and Cytology a minimum of 7ml is required in Tube 2    

Tube 3 for Microbiology upto 10ml (Full tube)

Xanthochromia investigation requires a 4th tube containing 1ml of CSF.

The sample with the least amount of blood is prefered for Cytology.

CSF SPECIMEN INFORMATION – LABORATORY PROCESSING

Depending on the tests requested, 3-4 separate tubes will need to be provided.  Note that the tubes are numbered so that order of collection can be identified – this order should be carefully followed, so that the optimal sample can be used for the requested tests.  It is also important to consider the tests that are required, as this will determine how much CSF is needed in each tube.  See the matrix below for details.

TUBE 1 is used for Cytology.

TUBE 2 is used for Glucose, Protein and Flow Cytometry.

(centrifuged in the lab – the supernatant is used for Biochemistry analysis (glucose, protein) and the cellular pellet will be used for flow cytometry if required).

TUBE 3 is for Microbiological analysis (cell count, Gram stain and culture) and any further infectious testing (e.g. viral PCR, mycobacterial PCR and culture, cryptococcal antigen).

TUBE 4 will be required only for;

         (a) Creutzfeld-Jacob Disease (CJD) testing OR

(b) Xanthochromia analysis (wrap the tube in foil to prevent    sample deterioration due to light).

 

TESTS REQUIRED

MINIMUM REQUIRED VOLUME (mL)

TUBE 1

TUBE 2

TUBE 3

TUBE 4

CYTOLOGY. CHEMISTRY, MICRO/CULTURE

1.0-2.0

1.0

1.0-2.0

Nil

ADD FLOW CYTOMETRY

 

add 5.0-7.0

 

 

ADD OTHER INFECTIOUS MARKERS

 

 

add 2.0- 5.0-

 

ADD MYCOBACTERIAL CULTURE

 

 

Add 10.00

 

ADD CJD

 

 

 

1.0

ADD XANTHOCHROMIA

 

 

 

1.0 (foil)

 

Note, the ability to add further tests to a previously collected sample is dependent upon the volume provided.  Also, many tests (flow cytometry, cytology) can only be performed on fresh specimens.

 

Other specialised testing (e.g. oligoclonal bands) will be performed using the supplied samples.

 

Xanthochromia and CJD analysis cannot be performed on a blood-stained sample.

Processing Instructions

Must be stored in the fridge.

 

Must be stored in the fridge.

Out of hours CSF samples for routine testing can be stored in the AP refrigerator.

 

Notes

Out of hours CSF samples for routine testing can be stored in the AP refrigerator.

 

 

 


 Recent changes for Cerebrospinal Fluid - microscopy and culture

Recent changes for Cerebrospinal Fluid - microscopy and culture

Date
Field
Changed From
Changed To
27th April 2021
Notes

CSF SPECIMEN INFORMATION – LABORATORY PROCESSING

Depending on the tests requested, 3-4 separate tubes will need to be provided.  Note that the tubes are numbered so that order of collection can be identified – this order should be carefully followed, so that the optimal sample can be used for the requested tests.  It is also important to consider the tests that are required, as this will determine how much CSF is needed in each tube.  See the matrix below for details.

TUBE 1 is used for Cytology.

TUBE 2 is used for Glucose, Protein and Flow Cytometry.

(centrifuged in the lab – the supernatant is used for Biochemistry analysis (glucose, protein) and the cellular pellet will be used for flow cytometry if required).

TUBE 3 is for Microbiological analysis (cell count, Gram stain and culture) and any further infectious testing (e.g. viral PCR, mycobacterial PCR and culture, cryptococcal antigen).

TUBE 4 will be required only for;

         (a) Creutzfeld-Jacob Disease (CJD) testing OR

(b) Xanthochromia analysis (wrap the tube in foil to prevent    sample deterioration due to light).

 

TESTS REQUIRED

MINIMUM REQUIRED VOLUME (mL)

TUBE 1

TUBE 2

TUBE 3

TUBE 4

CYTOLOGY. CHEMISTRY, MICRO/CULTURE

1.0-2.0

1.0

1.0-2.0

Nil

ADD FLOW CYTOMETRY

 

add 5.0-7.0

 

 

ADD OTHER INFECTIOUS MARKERS

 

 

add 2.0- 5.0-

 

ADD MYCOBACTERIAL CULTURE

 

 

Add 10.00

 

ADD CJD

 

 

 

1.0

ADD XANTHOCHROMIA

 

 

 

1.0 (foil)

 

Note, the ability to add further tests to a previously collected sample is dependent upon the volume provided.  Also, many tests (flow cytometry, cytology) can only be performed on fresh specimens.

 

Other specialised testing (e.g. oligoclonal bands) will be performed using the supplied samples.

 

Xanthochromia and CJD analysis cannot be performed on a blood-stained sample.

Special tests available on request:

PCR for viruses

Acid-fast stain and/or culture for Mycobacteria

Cryptococcal antigen

Serological test for Syphilis (must be paired with 5mL serum sample or test will not be performed)

Viral Serology, if CSF is accompanied by serum sample

Flow Cytometry - requires a 4th tube

The turnaround time for CSF microscopy are 30-40 minutes.

The turnaround time for CSF culture are 3-5 days.

Depending on the tests requested, 3-4 separate tubes will need to be provided. Note that the tubes are numbered so that order of collection can be identified – this order should be carefully followed, so that the optimal sample can be used for the requested tests. It is also important to consider the tests that are required, as this will determine how much CSF is needed in each tube. See the matrix below for details.

TUBE 1 is used for Biochemistry and Flow Cytometry. (Centrifuged in the lab – the supernatant is used for Biochemistry analysis (glucose, protein) and the cellular pellet will be used for flow cytometry if required). If tube 3 is bloodstained, take an aliquot for microbiology.

TUBE 2 is used for Cytology

TUBE 3 is for Microbiological analysis (cell count, Gram stain and culture) and any further infectious testing (e.g. viral PCR, mycobacterial PCR and culture, cryptococcal antigen).

TUBE 4 will be required only for;

(a) Creutzfeld-Jacob Disease (CJD) testing OR

(b) Xanthochromia analysis (wrap the tube in foil to prevent sample deterioration due to light).

 

TESTS REQUIRED

MINIMUM REQUIRED VOLUME (mL)

TUBE 1

TUBE 2

TUBE 3

TUBE 4

CHEMISTRY

1.0

     

ADD FLOW CYTOMETRY

Minimum 5.0-7.0

     

CYTOLOGY

 

1.0-3.0

   

MICROBIOLOGY

   

1.0-2.0

 

ADD OTHER INFECTIOUS MARKERS

   

add 2.0- 5.0-

 

ADD MYCOBACTERIAL CULTURE

   

Add 6.0-10.00

 

ADD CJD

     

1.0

ADD XANTHOCHROMIA

     

1.0 (foil)

 

Note, the ability to add further tests to a previously collected sample is dependent upon the volume provided. Also, many tests (flow cytometry, cytology) can only be performed on fresh specimens.

 

Other specialised testing (e.g. oligoclonal bands) will be performed using the supplied samples.

Xanthochromia and CJD analysis cannot be performed on a blood-stained sample.

14th August 2020
Notes

Special tests available on request:

PCR for viruses

Acid-fast stain and/or culture for Mycobacteria

Cryptococcal antigen

Serological test for Syphilis (must be paired with 5mL serum sample or test will not be performed)

Viral Serology, if CSF is accompanied by serum sample

Flow Cytometry - requires a 4th tube

The turnaround time for CSF microscopy are 30-40 minutes.

The turnaround time for CSF culture are 3-5 days.

CSF SPECIMEN INFORMATION – LABORATORY PROCESSING

Depending on the tests requested, 3-4 separate tubes will need to be provided.  Note that the tubes are numbered so that order of collection can be identified – this order should be carefully followed, so that the optimal sample can be used for the requested tests.  It is also important to consider the tests that are required, as this will determine how much CSF is needed in each tube.  See the matrix below for details.

TUBE 1 is used for Cytology.

TUBE 2 is used for Glucose, Protein and Flow Cytometry.

(centrifuged in the lab – the supernatant is used for Biochemistry analysis (glucose, protein) and the cellular pellet will be used for flow cytometry if required).

TUBE 3 is for Microbiological analysis (cell count, Gram stain and culture) and any further infectious testing (e.g. viral PCR, mycobacterial PCR and culture, cryptococcal antigen).

TUBE 4 will be required only for;

         (a) Creutzfeld-Jacob Disease (CJD) testing OR

(b) Xanthochromia analysis (wrap the tube in foil to prevent    sample deterioration due to light).

 

TESTS REQUIRED

MINIMUM REQUIRED VOLUME (mL)

TUBE 1

TUBE 2

TUBE 3

TUBE 4

CYTOLOGY. CHEMISTRY, MICRO/CULTURE

1.0-2.0

1.0

1.0-2.0

Nil

ADD FLOW CYTOMETRY

 

add 5.0-7.0

 

 

ADD OTHER INFECTIOUS MARKERS

 

 

add 2.0- 5.0-

 

ADD MYCOBACTERIAL CULTURE

 

 

Add 10.00

 

ADD CJD

 

 

 

1.0

ADD XANTHOCHROMIA

 

 

 

1.0 (foil)

 

Note, the ability to add further tests to a previously collected sample is dependent upon the volume provided.  Also, many tests (flow cytometry, cytology) can only be performed on fresh specimens.

 

Other specialised testing (e.g. oligoclonal bands) will be performed using the supplied samples.

 

Xanthochromia and CJD analysis cannot be performed on a blood-stained sample.

Special tests available on request:

PCR for viruses

Acid-fast stain and/or culture for Mycobacteria

Cryptococcal antigen

Serological test for Syphilis (must be paired with 5mL serum sample or test will not be performed)

Viral Serology, if CSF is accompanied by serum sample

Flow Cytometry - requires a 4th tube

The turnaround time for CSF microscopy are 30-40 minutes.

The turnaround time for CSF culture are 3-5 days.


 Recent changes for Cerebrospinal Fluid Specimen Collection

Recent changes for Cerebrospinal Fluid Specimen Collection

Date
Field
Changed From
Changed To
27th April 2021
Notes

Depending on the tests requested, 3-4 separate tubes will need to be provided. Note that the tubes are numbered so that order of collection can be identified – this order should be carefully followed, so that the optimal sample can be used for the requested tests. It is also important to consider the tests that are required, as this will determine how much CSF is needed in each tube. See the matrix below for details.

TUBE 1 is used for Biochemistry and Flow Cytometry. (Centrifuged in the lab – the supernatant is used for Biochemistry analysis (glucose, protein) and the cellular pellet will be used for flow cytometry if required). If tube 3 is bloodstained, take an aliquot for microbiology.

TUBE 2 is used for Cytology

TUBE 3 is for Microbiological analysis (cell count, Gram stain and culture) and any further infectious testing (e.g. viral PCR, mycobacterial PCR and culture, cryptococcal antigen).

TUBE 4 will be required only for;

(a) Creutzfeld-Jacob Disease (CJD) testing OR

(b) Xanthochromia analysis (wrap the tube in foil to prevent sample deterioration due to light).

 

TESTS REQUIRED

MINIMUM REQUIRED VOLUME (mL)

TUBE 1

TUBE 2

TUBE 3

TUBE 4

CHEMISTRY

1.0

     

ADD FLOW CYTOMETRY

Minimum 5.0-7.0

     

CYTOLOGY

 

1.0-3.0

   

MICROBIOLOGY

   

1.0-2.0

 

ADD OTHER INFECTIOUS MARKERS

   

add 2.0- 5.0-

 

ADD MYCOBACTERIAL CULTURE

   

Add 6.0-10.00

 

ADD CJD

     

1.0

ADD XANTHOCHROMIA

     

1.0 (foil)

 

Note, the ability to add further tests to a previously collected sample is dependent upon the volume provided. Also, many tests (flow cytometry, cytology) can only be performed on fresh specimens.

 

Other specialised testing (e.g. oligoclonal bands) will be performed using the supplied samples.

Xanthochromia and CJD analysis cannot be performed on a blood-stained sample.

 

 

Depending on the tests requested, 3-4 separate tubes will need to be provided. Note that the tubes are numbered so that order of collection can be identified – this order should be carefully followed, so that the optimal sample can be used for the requested tests. It is also important to consider the tests that are required, as this will determine how much CSF is needed in each tube. See the matrix below for details.

TUBE 1 is used for Biochemistry and Flow Cytometry. (Centrifuged in the lab – the supernatant is used for Biochemistry analysis (glucose, protein) and the cellular pellet will be used for flow cytometry if required). If tube 3 is bloodstained, take an aliquot for microbiology.

TUBE 2 is used for Cytology

TUBE 3 is for Microbiological analysis (cell count, Gram stain and culture) and any further infectious testing (e.g. viral PCR, mycobacterial PCR and culture, cryptococcal antigen).

TUBE 4 will be required only for;

(a) Creutzfeld-Jacob Disease (CJD) testing OR

(b) Xanthochromia analysis (wrap the tube in foil to prevent sample deterioration due to light).

 

TESTS REQUIRED

MINIMUM REQUIRED VOLUME (mL)

TUBE 1

TUBE 2

TUBE 3

TUBE 4

CHEMISTRY

1.0

     

ADD FLOW CYTOMETRY

Minimum 5.0-7.0

     

CYTOLOGY

 

1.0-3.0

   

MICROBIOLOGY

   

1.0-2.0

 

ADD OTHER INFECTIOUS MARKERS

   

add 2.0- 5.0-

 

ADD MYCOBACTERIAL CULTURE

   

Add 6.0-10.00

 

ADD CJD

     

1.0

ADD XANTHOCHROMIA

     

1.0 (foil)

 

Note, the ability to add further tests to a previously collected sample is dependent upon the volume provided. Also, many tests (flow cytometry, cytology) can only be performed on fresh specimens.

 

Other specialised testing (e.g. oligoclonal bands) will be performed using the supplied samples.

Xanthochromia and CJD analysis cannot be performed on a blood-stained sample.

 

27th April 2021
Notes

CSF SPECIMEN INFORMATION – LABORATORY PROCESSING

Depending on the tests requested, 3-4 separate tubes will need to be provided.  Note that the tubes are numbered so that order of collection can be identified – this order should be carefully followed, so that the optimal sample can be used for the requested tests.  It is also important to consider the tests that are required, as this will determine how much CSF is needed in each tube.  See the matrix below for details.

TUBE 1 is used for Cytology.

TUBE 2 is used for Glucose, Protein and Flow Cytometry.

(centrifuged in the lab – the supernatant is used for Biochemistry analysis (glucose, protein) and the cellular pellet will be used for flow cytometry if required).

TUBE 3 is for Microbiological analysis (cell count, Gram stain and culture) and any further infectious testing (e.g. viral PCR, mycobacterial PCR and culture, cryptococcal antigen).

TUBE 4 will be required only for;

         (a) Creutzfeld-Jacob Disease (CJD) testing OR

(b) Xanthochromia analysis (wrap the tube in foil to prevent    sample deterioration due to light).

 

TESTS REQUIRED

MINIMUM REQUIRED VOLUME (mL)

TUBE 1

TUBE 2

TUBE 3

TUBE 4

CYTOLOGY. CHEMISTRY, MICRO/CULTURE

1.0-2.0

1.0

1.0-2.0

Nil

ADD FLOW CYTOMETRY

 

add 5.0-7.0

 

 

ADD OTHER INFECTIOUS MARKERS

 

 

add 2.0- 5.0-

 

ADD MYCOBACTERIAL CULTURE

 

 

Add 10.00

 

ADD CJD

 

 

 

1.0

ADD XANTHOCHROMIA

 

 

 

1.0 (foil)

 

Note, the ability to add further tests to a previously collected sample is dependent upon the volume provided.  Also, many tests (flow cytometry, cytology) can only be performed on fresh specimens.

 

Other specialised testing (e.g. oligoclonal bands) will be performed using the supplied samples.

 

Xanthochromia and CJD analysis cannot be performed on a blood-stained sample.

Depending on the tests requested, 3-4 separate tubes will need to be provided. Note that the tubes are numbered so that order of collection can be identified – this order should be carefully followed, so that the optimal sample can be used for the requested tests. It is also important to consider the tests that are required, as this will determine how much CSF is needed in each tube. See the matrix below for details.

TUBE 1 is used for Biochemistry and Flow Cytometry. (Centrifuged in the lab – the supernatant is used for Biochemistry analysis (glucose, protein) and the cellular pellet will be used for flow cytometry if required). If tube 3 is bloodstained, take an aliquot for microbiology.

TUBE 2 is used for Cytology

TUBE 3 is for Microbiological analysis (cell count, Gram stain and culture) and any further infectious testing (e.g. viral PCR, mycobacterial PCR and culture, cryptococcal antigen).

TUBE 4 will be required only for;

(a) Creutzfeld-Jacob Disease (CJD) testing OR

(b) Xanthochromia analysis (wrap the tube in foil to prevent sample deterioration due to light).

 

TESTS REQUIRED

MINIMUM REQUIRED VOLUME (mL)

TUBE 1

TUBE 2

TUBE 3

TUBE 4

CHEMISTRY

1.0

     

ADD FLOW CYTOMETRY

Minimum 5.0-7.0

     

CYTOLOGY

 

1.0-3.0

   

MICROBIOLOGY

   

1.0-2.0

 

ADD OTHER INFECTIOUS MARKERS

   

add 2.0- 5.0-

 

ADD MYCOBACTERIAL CULTURE

   

Add 6.0-10.00

 

ADD CJD

     

1.0

ADD XANTHOCHROMIA

     

1.0 (foil)

 

Note, the ability to add further tests to a previously collected sample is dependent upon the volume provided. Also, many tests (flow cytometry, cytology) can only be performed on fresh specimens.

 

Other specialised testing (e.g. oligoclonal bands) will be performed using the supplied samples.

Xanthochromia and CJD analysis cannot be performed on a blood-stained sample.

 

 

14th August 2020
Notes

Collect CSF into three separate, sterile, screw-capped tubes provided (labelled 1,2 & 3)

Tube 1 is for Biochemistry (for Protein and Glucose) 0.5ml (10 drops) required 

Tube 2 for Cytology 1.0ml (20 drops)  and/or Flow Cytometry (6ml)

For Flow Cytometry a minimum of 6ml is required  in Tube 2

For Flow Cytometry and Cytology a minimum of 7ml is required in Tube 2     

Tube 3 for Microbiology upto 10ml (Full tube)

If querying CJD the laboratory requires a 4th unopened tube containing at least 1ml of non-bloodstained CSF.

Xanthochromia investigation requires a 4th tube containing 1ml of CSF .

Cell Reference Ranges

                  Adults:

                  0 - 5 lymphocytes (x10*6/L)

                  0  neutrophils

                   Neonates:

                  (£ 1 month)

                  0 – 20 white cells (x10*6/L, predominantly lymphocytes)

                  0 - several hundred erythrocytes


Leucocyte:erythrocyte (WBC:RBC) ratio is normally 1:500 (including traumatic tap).

 

 

CSF SPECIMEN INFORMATION – LABORATORY PROCESSING

Depending on the tests requested, 3-4 separate tubes will need to be provided.  Note that the tubes are numbered so that order of collection can be identified – this order should be carefully followed, so that the optimal sample can be used for the requested tests.  It is also important to consider the tests that are required, as this will determine how much CSF is needed in each tube.  See the matrix below for details.

TUBE 1 is used for Cytology.

TUBE 2 is used for Glucose, Protein and Flow Cytometry.

(centrifuged in the lab – the supernatant is used for Biochemistry analysis (glucose, protein) and the cellular pellet will be used for flow cytometry if required).

TUBE 3 is for Microbiological analysis (cell count, Gram stain and culture) and any further infectious testing (e.g. viral PCR, mycobacterial PCR and culture, cryptococcal antigen).

TUBE 4 will be required only for;

         (a) Creutzfeld-Jacob Disease (CJD) testing OR

(b) Xanthochromia analysis (wrap the tube in foil to prevent    sample deterioration due to light).

 

TESTS REQUIRED

MINIMUM REQUIRED VOLUME (mL)

TUBE 1

TUBE 2

TUBE 3

TUBE 4

CYTOLOGY. CHEMISTRY, MICRO/CULTURE

1.0-2.0

1.0

1.0-2.0

Nil

ADD FLOW CYTOMETRY

 

add 5.0-7.0

 

 

ADD OTHER INFECTIOUS MARKERS

 

 

add 2.0- 5.0-

 

ADD MYCOBACTERIAL CULTURE

 

 

Add 10.00

 

ADD CJD

 

 

 

1.0

ADD XANTHOCHROMIA

 

 

 

1.0 (foil)

 

Note, the ability to add further tests to a previously collected sample is dependent upon the volume provided.  Also, many tests (flow cytometry, cytology) can only be performed on fresh specimens.

 

Other specialised testing (e.g. oligoclonal bands) will be performed using the supplied samples.

 

Xanthochromia and CJD analysis cannot be performed on a blood-stained sample.


 Recent changes for Chromium

Recent changes for Chromium

Date
Field
Changed From
Changed To
12th February 2020
Preferred Container Type

Trace metal tube ( Dark blue )

Trace Metal with Aliquot

Collection & Request Instructions

LAB STAFF NOTE : The Reference Lab will only receive aliquots which are sent in a metal-free aliquot tube (see container picture above).  Sample must be decanted by pour-off (do not use plastic pipettes). 


 Recent changes for Coagulation Factor Assays II, V, VII, VIII, IX, X, XI, XII

Recent changes for Coagulation Factor Assays II, V, VII, VIII, IX, X, XI, XII

Date
Field
Changed From
Changed To
10th December 2020
Collection & Request Instructions

Do not use 1.0 mL citrate tubes

Collect 1 X 3.5 ml Sodium Citrate tube is required to run all Factor assay ( Excent Factor XIII which is a refered test).

1 Tube is sufficient for performing these tests.
 

Do not use 1.0 mL citrate tubes

Collect 1 X 3.5 ml Sodium Citrate tube is sufficient to run all Factor assay ( Excent Factor XIII which is a refered test).

1 Tube is sufficient for performing these tests.
 

10th December 2020
Collection & Request Instructions

Do not use 1.0 mL citrate tubes

Do not use 1.0 mL citrate tubes

Collect 1 X 3.5 ml Sodium Citrate tube is required to run all Factor assay ( Excent Factor XIII which is a refered test).

1 Tube is sufficient for performing these tests.
 


 Recent changes for Collagen Binding Assay

Recent changes for Collagen Binding Assay

Date
Field
Changed From
Changed To
11th June 2021
Laboratory

Referred Test

Coagulation

Request Group

CBA

Collection & Request Instructions

Fill to label marker and mix by inversion.

Do not use 1 mL citrate tubes.

Fill to label marker and mix by inversion.

Do not use 1 mL citrate tubes.

Processing Instructions

Specimens kept at room temperature, should be centrifuged ASAP and tested within 4 hours from time of specimen collection. If testing is not completed within 4 hours, plasma should be removed from cells and aliquoted (1mL) into Eppendorf tubes, 2 X 1 mL vials per CBA assay.

Plasma may be frozen @ -20 C for up to 2 weeks or -70 C for up to 6 months. Frost free freezers should not be used.

Specimens kept at room temperature, should be centrifuged ASAP and tested within 4 hours from time of specimen collection. If testing is not completed within 4 hours, plasma should be removed from cells and aliquoted (1mL) into Eppendorf tubes, 2 X 1 mL vials per CBA assay.

Plasma may be frozen @ -20 C for up to 2 weeks or -70 C for up to 6 months. Frost free freezers should not be used.

External Laboratory

Austin Pathology

Frequency

Fortnightly


 Recent changes for Copper-Liver

Recent changes for Copper-Liver

Date
Field
Changed From
Changed To
10th May 2021
External Laboratory

Australian Clinical Labs (formerly Healthscope)

Pacific Laboratory Medicine Services


 Recent changes for Coronavirus PCR

Recent changes for Coronavirus PCR

Date
Field
Changed From
Changed To
24th January 2020
Alternative names

extended panel PCR, MERS PCR, novel coronavirus PCR, Respiratory Virus NAD, SARS PCR

extended panel PCR, MERS PCR, novel coronavirus PCR, Respiratory Virus NAD, SARS PCR, novel Wuhan

24th January 2020
Alternative names

Respiratory Virus NAD, SARS PCR

Respiratory Virus NAD, SARS PCR, extended panel PCR, novel coronavirus PCR, MERS PCR

Request Group

RESPCR

RESPX


 Recent changes for COVID-19 Antibody

Recent changes for COVID-19 Antibody

Date
Field
Changed From
Changed To
24th September 2020
Assay name

covab

COVAB

Alternative names

SARS-CoV-2 Antibody COVID Antibody COVID Serology

Laboratory

Unassigned

Biochemistry

Request Group

COVAB

Collection & Request Instructions

Transport specimen at Room Temperature to lab

Frequency

Daily


 Recent changes for COVID-19 PCR

Recent changes for COVID-19 PCR

Date
Field
Changed From
Changed To
16th September 2020
External Laboratory

Victorian Infectious Diseases Reference Laboratory (V.I.D.R.L)

16th September 2020
Collection & Request Instructions

Nasopharyngeal swab in RED Copan viral transport medium

Nasal/oropharyngeal swab in GREEN Kang Jian viral transport medium

Nasal/oropharyngeal swab in GREEN Kang Jian viral transport medium

Paediatric - Nasopharyngeal swab in RED Copan viral transport medium

Notes

For Rapid testing from Emergency Department - swab must be accompanied by signed Rapid Test Authorisation Form.

DO NOT call the laboratory for results/enquiries. Inpatient tests will be prioritised and results available on EMR ASAP.

Sputum samples are referred to an external laboratory - results will be delayed.

Samples MUST be in a clear specimen bag within an outer GREEN specimen bag

DO NOT send samples in the Pathology Chute

For Rapid testing from Emergency Department - swab must be accompanied by signed Rapid Test Authorisation Form.

DO NOT call the laboratory for results/enquiries. Inpatient tests will be prioritised and results available on EMR ASAP.

Sputum samples are referred to an external laboratory - results will be delayed.

16th September 2020
Preferred Specimen Type

Nasopharyngeal swab
NTS - nose & throat swab

Nasopharyngeal swab
NTS - nose & throat swab
Sputum

Preferred Container Type

Viral Transport Media - Nasopharyngeal
respiratory PCR requirements
See Instructions

Viral Transport Media - Nasopharyngeal
See Instructions
Sterile Jar

Notes

For Rapid testing from Emergency Department - swab must be accompanied by signed Rapid Test Authorisation Form.

DO NOT call the laboratory for results/enquiries. Inpatient tests will be prioritised and results available on EMR.

For Rapid testing from Emergency Department - swab must be accompanied by signed Rapid Test Authorisation Form.

DO NOT call the laboratory for results/enquiries. Inpatient tests will be prioritised and results available on EMR ASAP.

Sputum samples are referred to an external laboratory - results will be delayed.


 Recent changes for Cyclosporin A

Recent changes for Cyclosporin A

Date
Field
Changed From
Changed To
6th May 2020
Method

PLEASE NOTE : CYCLOSPORIN REFERRALS MUST ONLY BE SENT TO THE AUSTIN - THEY MUSY NOT BE SENT TO THE ALFRED AS THEY USE A METHOD THAT DOES NOT CORRESPOND WITH THE EXPECTED REFEERNCE RANGES.

PLEASE NOTE : CYCLOSPORIN send to the Austin Biochemistry Department.

6th May 2020
Collection & Request Instructions

In post transplant patients a 2 hour post dose is the preferred specimen, please indicate dosing information on the request slip.

Specimen must arrive at the Box Hill laboratory before 1200 Mon-Fri.

Separate EDTA specimen required.

 

In post transplant patients a 2 hour post dose is the preferred specimen, please indicate dosing information on the request slip.

Specimen must arrive at the Box Hill laboratory before 1200 everyday.

Separate EDTA specimen required.

 


 Recent changes for DNA analysis for Thalassaemia (related to Hgb Electrophoresis)

Recent changes for DNA analysis for Thalassaemia (related to Hgb Electrophoresis)

Date
Field
Changed From
Changed To
10th December 2020
Collection & Request Instructions

Send FBE, Iron studies and Hb electrophoresis results.

Send within 24 Hrs.

Results: 95943398

Please collect 2x EDTA tube for this test.

Send FBE, Iron studies and Hb electrophoresis results.

Send within 24 Hrs.

Results: 95943398


 Recent changes for Euglobulin Clot Lysis Time

Recent changes for Euglobulin Clot Lysis Time

Date
Field
Changed From
Changed To
10th December 2020
Volume (Adults)

Method

This test is no longer performed. Please contact Haematologist or Senior Scientist in Haematology if further information is required.


 Recent changes for Fluconazole

Recent changes for Fluconazole

Date
Field
Changed From
Changed To
14th October 2021
Collection & Request Instructions

 

 

 

 

Processing Instructions

Spin, separate Plasma and freeze.

Packed by certified personnel.

Results:02-83829100 or 02-82829190

Spin and separate Plasma.

 

External Laboratory

Sydpath Pathology, St Vincent's Hospital NSW

Alfred Hospital


 Recent changes for Free PSA

Recent changes for Free PSA

Date
Field
Changed From
Changed To
21st June 2021
External Laboratory

Melbourne Pathology

Method

Free PSA is now performed in house at Box Hill Hospital.

Chemical Pathologist approval is still required


 Recent changes for Haemoglobin Analysis (including Electrophoresis)

Recent changes for Haemoglobin Analysis (including Electrophoresis)

Date
Field
Changed From
Changed To
10th December 2020
Alternative names

Alkali Resistant Haemoglobin, Foetal Haemoglobin, Hb EPG, HbA2, HbF, HbH, HbS, Unstable Haemoglobin

Alkali Resistant Haemoglobin, Foetal Haemoglobin, Hb EPG, HbA2, HbF, HbH, HbS, Unstable Haemoglobin, Thalassaemia

Collection & Request Instructions

Please collect 2 X EDTA Tube for this test.


 Recent changes for Herpes Virus PCR

Recent changes for Herpes Virus PCR

Date
Field
Changed From
Changed To
19th October 2020
External Transport Instructions

Tests included in Monash PCR - Herpes simplex 1 virus, Herpes simplex 2 virus, Varicella zoster virus, Cytomegalovirus, Enterovirus (swabs only), Adenovirus (not faeces)

Specimens tested at Monash-

Swabs (dry or in UTM) serum, Plasma (ACD or EDTA), Sterile fluids, Respiratory samples, Urine.

Specimens anticoagulated with heparin are unsuitable as heparin inhibits PCR

For other specimens - refer to VIDRL. Tissue, CSF, saliva.

If  CSF Herpes and Enterovirus PCR -send to VIDRL.

Refer to Referred Molecular Test Requests WORK-MI-90

Tests included in Monash PCR - Herpes simplex 1 virus, Herpes simplex 2 virus, Varicella zoster virus, Cytomegalovirus, Enterovirus (swabs only), Adenovirus (not faeces)

Specimens tested at Monash-

Swabs (dry or in UTM), Sterile fluids, Respiratory samples, Urine.

Specimens anticoagulated with heparin are unsuitable as heparin inhibits PCR

For other specimens - refer to VIDRL. Tissue, CSF, saliva, serum, Plasma (ACD or EDTA),

If  CSF Herpes and Enterovirus PCR -send to VIDRL.

Refer to Referred Molecular Test Requests WORK-MI-90


 Recent changes for HMG-CoA Reductase

Recent changes for HMG-CoA Reductase

Date
Field
Changed From
Changed To
7th February 2020
Alternative names

3-Hydroxy-3-Methylglutaryl-coenzyme A Reductase), Anti-HMGCR, Autoantibodies to HMGCR

3-Hydroxy-3-Methylglutaryl-coenzyme A Reductase, Anti-HMGCR, Autoantibodies to HMGCR


 Recent changes for Holotranscobalamin

Recent changes for Holotranscobalamin

Date
Field
Changed From
Changed To
24th February 2020
Processing Instructions

HoloTC is only stable for 3 days at 2-8 C, hence if it is being stored at an Eastern Health Lab over the weekend, freeze and transport frozen.

External Transport Instructions

Please send sample at 4 degrees C. for weekend storage please freeze.

Frequency

Daily

12th February 2020
Laboratory

Referred Test

Biochemistry

External Laboratory

Alfred Hospital


 Recent changes for IgE, Total

Recent changes for IgE, Total

Date
Field
Changed From
Changed To
3rd December 2019
Laboratory

Referred Test

Biochemistry

External Laboratory

St Vincents Hospital


 Recent changes for Iron - Liver

Recent changes for Iron - Liver

Date
Field
Changed From
Changed To
10th May 2021
Collection & Request Instructions

Liver biopsy specimen to be placed directly into a clear, dry container and delivered to the laboraory as soon as possible. Minimum sample size for accurate analysis is 1-2 mg.

Liver biopsy specimen to be placed directly into a clear, dry container and delivered to the laboraory as soon as possible. Minimum sample size for accurate analysis is 1-2 mg.

External Laboratory

Australian Clinical Labs (formerly Healthscope)

Pacific Laboratory Medicine Services

Notes

Hepatic Iron Index

Hepatic Iron Concentration

 


 

Age in years

Values > 2 indicate haemochromatosis

Liver biopsy specimen to be placed directly into a clear, dry container and delivered to the laboraory as soon as possible. Minimum sample size for accurate analysis is 1-2 mg.

 

Hepatic Iron Index

Hepatic Iron Concentration

 

 

Age in years

Values > 2 indicate haemochromatosis

Liver biopsy specimen to be placed directly into a clear, dry container and delivered to the laboraory as soon as possible. Minimum sample size for accurate analysis is 1-2 mg.


 Recent changes for Leucocyte Immunophenotyping

Recent changes for Leucocyte Immunophenotyping

Date
Field
Changed From
Changed To
15th October 2021
Notes

Samples must be marked 'URGENT', must reach the laboratory as soon as possible. 

All out-of-hours testing requires notification.

Time and date of collection must be recorded on request form.

Whole blood - 1 x EDTA sample, do not spin. Store at room temperature. An FBE must also be requested,  and needs a separate EDTA sample.

Bone marrow aspirate - Sample into  EDTA tube

TissueFresh (if to be shared with other departments for other tests eg. Histopathology or Microbiology) or RPMI fluid (especially if specimen is taken out of hours). This fluid is obtainable from: BH Lab 9895 3884, MH Lab: 9871 3572 or AH Lab: 9764 6136.

CSF/other fluids

Depending on the tests requested, 3-4 separate tubes will need to be provided. Note that the tubes are numbered so that order of collection can be identified – this order should be carefully followed, so that the optimal sample can be used for the requested tests. It is also important to consider the tests that are required, as this will determine how much CSF is needed in each tube. See the matrix below for details.

TUBE 1 is used for Biochemistry and Flow Cytometry. (Centrifuged in the lab – the supernatant is used for Biochemistry analysis (glucose, protein) and the cellular pellet will be used for flow cytometry if required). If tube 3 is bloodstained, take an aliquot for microbiology.

TUBE 2 is used for Cytology

TUBE 3 is for Microbiological analysis (cell count, Gram stain and culture) and any further infectious testing (e.g. viral PCR, mycobacterial PCR and culture, cryptococcal antigen).

TUBE 4 will be required only for;

(a) Creutzfeld-Jacob Disease (CJD) testing OR

(b) Xanthochromia analysis (wrap the tube in foil to prevent sample deterioration due to light).

 

TESTS REQUIRED

MINIMUM REQUIRED VOLUME (mL)

TUBE 1

TUBE 2

TUBE 3

TUBE 4

CHEMISTRY

1.0

 

 

 

ADD FLOW CYTOMETRY

Minimum 5.0-7.0

 

 

 

CYTOLOGY

 

1.0-3.0

 

 

MICROBIOLOGY

 

 

1.0-2.0

 

ADD OTHER INFECTIOUS MARKERS

 

 

add 2.0- 5.0-

 

ADD MYCOBACTERIAL CULTURE

 

 

Add 6.0-10.00

 

ADD CJD

 

 

 

1.0

ADD XANTHOCHROMIA

 

 

 

1.0 (foil)

 

Note, the ability to add further tests to a previously collected sample is dependent upon the volume provided. Also, many tests (flow cytometry, cytology) can only be performed on fresh specimens.

 

Other specialised testing (e.g. oligoclonal bands) will be performed using the supplied samples.

Xanthochromia and CJD analysis cannot be performed on a blood-stained sample

Samples must be marked 'URGENT', must reach the laboratory as soon as possible. 

All out-of-hours testing requires notification.

Time and date of collection must be recorded on request form.

Whole blood - 1 x EDTA sample, do not spin. Store at room temperature. An FBE and BLOOD FILM must also be requested,  and needs a separate EDTA sample.

Bone marrow aspirate - Sample into  EDTA tube

TissueFresh (if to be shared with other departments for other tests eg. Histopathology or Microbiology) or RPMI fluid (especially if specimen is taken out of hours). This fluid is obtainable from: BH Lab 9895 3884, MH Lab: 9871 3572 or AH Lab: 9764 6136.

CSF/other fluids

Depending on the tests requested, 3-4 separate tubes will need to be provided. Note that the tubes are numbered so that order of collection can be identified – this order should be carefully followed, so that the optimal sample can be used for the requested tests. It is also important to consider the tests that are required, as this will determine how much CSF is needed in each tube. See the matrix below for details.

TUBE 1 is used for Biochemistry and Flow Cytometry. (Centrifuged in the lab – the supernatant is used for Biochemistry analysis (glucose, protein) and the cellular pellet will be used for flow cytometry if required). If tube 3 is bloodstained, take an aliquot for microbiology.

TUBE 2 is used for Cytology

TUBE 3 is for Microbiological analysis (cell count, Gram stain and culture) and any further infectious testing (e.g. viral PCR, mycobacterial PCR and culture, cryptococcal antigen).

TUBE 4 will be required only for;

(a) Creutzfeld-Jacob Disease (CJD) testing OR

(b) Xanthochromia analysis (wrap the tube in foil to prevent sample deterioration due to light).

 

TESTS REQUIRED

MINIMUM REQUIRED VOLUME (mL)

TUBE 1

TUBE 2

TUBE 3

TUBE 4

CHEMISTRY

1.0

 

 

 

ADD FLOW CYTOMETRY

Minimum 5.0-7.0

 

 

 

CYTOLOGY

 

1.0-3.0

 

 

MICROBIOLOGY

 

 

1.0-2.0

 

ADD OTHER INFECTIOUS MARKERS

 

 

add 2.0- 5.0-

 

ADD MYCOBACTERIAL CULTURE

 

 

Add 6.0-10.00

 

ADD CJD

 

 

 

1.0

ADD XANTHOCHROMIA

 

 

 

1.0 (foil)

 

Note, the ability to add further tests to a previously collected sample is dependent upon the volume provided. Also, many tests (flow cytometry, cytology) can only be performed on fresh specimens.

 

Other specialised testing (e.g. oligoclonal bands) will be performed using the supplied samples.

Xanthochromia and CJD analysis cannot be performed on a blood-stained sample

14th October 2021
Notes

 

 

Samples must be marked 'URGENT', must reach the laboratory as soon as possible. 

All out-of-hours testing requires notification.

Time and date of collection must be recorded on request form.

Whole blood - 1 x EDTA sample, do not spin. Store at room temperature. An FBE must also be requested,  and needs a separate EDTA sample.

Bone marrow aspirate - Sample into  EDTA tube

TissueFresh (if to be shared with other departments for other tests eg. Histopathology or Microbiology) or RPMI fluid (especially if specimen is taken out of hours). This fluid is obtainable from: BH Lab 9895 3884, MH Lab: 9871 3572 or AH Lab: 9764 6136.

CSF/other fluids - at least 2mL is preferred

Depending on the tests requested, 3-4 separate tubes will need to be provided. Note that the tubes are numbered so that order of collection can be identified – this order should be carefully followed, so that the optimal sample can be used for the requested tests. It is also important to consider the tests that are required, as this will determine how much CSF is needed in each tube. See the matrix below for details.

TUBE 1 is used for Biochemistry and Flow Cytometry. (Centrifuged in the lab – the supernatant is used for Biochemistry analysis (glucose, protein) and the cellular pellet will be used for flow cytometry if required). If tube 3 is bloodstained, take an aliquot for microbiology.

TUBE 2 is used for Cytology

TUBE 3 is for Microbiological analysis (cell count, Gram stain and culture) and any further infectious testing (e.g. viral PCR, mycobacterial PCR and culture, cryptococcal antigen).

TUBE 4 will be required only for;

(a) Creutzfeld-Jacob Disease (CJD) testing OR

(b) Xanthochromia analysis (wrap the tube in foil to prevent sample deterioration due to light).

 

TESTS REQUIRED

MINIMUM REQUIRED VOLUME (mL)

TUBE 1

TUBE 2

TUBE 3

TUBE 4

CHEMISTRY

1.0

 

 

 

ADD FLOW CYTOMETRY

Minimum 5.0-7.0

 

 

 

CYTOLOGY

 

1.0-3.0

 

 

MICROBIOLOGY

 

 

1.0-2.0

 

ADD OTHER INFECTIOUS MARKERS

 

 

add 2.0- 5.0-

 

ADD MYCOBACTERIAL CULTURE

 

 

Add 6.0-10.00

 

ADD CJD

 

 

 

1.0

ADD XANTHOCHROMIA

 

 

 

1.0 (foil)

 

Note, the ability to add further tests to a previously collected sample is dependent upon the volume provided. Also, many tests (flow cytometry, cytology) can only be performed on fresh specimens.

 

Other specialised testing (e.g. oligoclonal bands) will be performed using the supplied samples.

Xanthochromia and CJD analysis cannot be performed on a blood-stained sample

 

 

Samples must be marked 'URGENT', must reach the laboratory as soon as possible. 

All out-of-hours testing requires notification.

Time and date of collection must be recorded on request form.

Whole blood - 1 x EDTA sample, do not spin. Store at room temperature. An FBE must also be requested,  and needs a separate EDTA sample.

Bone marrow aspirate - Sample into  EDTA tube

TissueFresh (if to be shared with other departments for other tests eg. Histopathology or Microbiology) or RPMI fluid (especially if specimen is taken out of hours). This fluid is obtainable from: BH Lab 9895 3884, MH Lab: 9871 3572 or AH Lab: 9764 6136.

CSF/other fluids

Depending on the tests requested, 3-4 separate tubes will need to be provided. Note that the tubes are numbered so that order of collection can be identified – this order should be carefully followed, so that the optimal sample can be used for the requested tests. It is also important to consider the tests that are required, as this will determine how much CSF is needed in each tube. See the matrix below for details.

TUBE 1 is used for Biochemistry and Flow Cytometry. (Centrifuged in the lab – the supernatant is used for Biochemistry analysis (glucose, protein) and the cellular pellet will be used for flow cytometry if required). If tube 3 is bloodstained, take an aliquot for microbiology.

TUBE 2 is used for Cytology

TUBE 3 is for Microbiological analysis (cell count, Gram stain and culture) and any further infectious testing (e.g. viral PCR, mycobacterial PCR and culture, cryptococcal antigen).

TUBE 4 will be required only for;

(a) Creutzfeld-Jacob Disease (CJD) testing OR

(b) Xanthochromia analysis (wrap the tube in foil to prevent sample deterioration due to light).

 

TESTS REQUIRED

MINIMUM REQUIRED VOLUME (mL)

TUBE 1

TUBE 2

TUBE 3

TUBE 4

CHEMISTRY

1.0

 

 

 

ADD FLOW CYTOMETRY

Minimum 5.0-7.0

 

 

 

CYTOLOGY

 

1.0-3.0

 

 

MICROBIOLOGY

 

 

1.0-2.0

 

ADD OTHER INFECTIOUS MARKERS

 

 

add 2.0- 5.0-

 

ADD MYCOBACTERIAL CULTURE

 

 

Add 6.0-10.00

 

ADD CJD

 

 

 

1.0

ADD XANTHOCHROMIA

 

 

 

1.0 (foil)

 

Note, the ability to add further tests to a previously collected sample is dependent upon the volume provided. Also, many tests (flow cytometry, cytology) can only be performed on fresh specimens.

 

Other specialised testing (e.g. oligoclonal bands) will be performed using the supplied samples.

Xanthochromia and CJD analysis cannot be performed on a blood-stained sample

14th October 2021
Alternative names

CD4/CD8 Counts, Cell Surface Markers, FACS Analysis, Flow Cytometry

Cell Surface Markers, FACS Analysis, Flow Cytometry, Immunophenotyping

Request Group

FLOW

LYMP, LEUK

Collection & Request Instructions

Sample store at Room temperature. Samples are not stable for more than 24 hours. Hence they must be received by the Flow Cytometry lab at Box Hill within normal business hours Monday to Friday.

Cutoff for outpatient sample collection on Fridays is at 11.30 AM and MUST reach the laboratory before 4.00PM.

Flow cytometric analysis of cells stained by immunofluorescence with a range of antibodies against lymphoid and myeloid markers.

The panel of markers used will be appropriate to the clinical comments supplied , or as arranged by prior consultation.

Sample store at Room temperature. Samples are not stable for more than 24 hours. Hence they must be received by the Flow Cytometry lab at Box Hill within normal business hours Monday to Friday.

Cut-off for outpatient sample collection on Fridays is at 11.30 AM and MUST reach the laboratory before 4.00PM.

Flow cytometric analysis of cells stained by immunofluorescence with a range of antibodies against lymphoid and myeloid markers.

The panel of markers used will be appropriate to the panel selected and the clinical comments supplied , or as arranged by prior consultation

Notes

 

 

Samples must be marked 'URGENT', must reach the laboratory as soon as possible. 

All out-of-hours testing requires notification.

Time and date of collection must be recorded on request form.

Whole blood - 1 x EDTA sample, do not spin. Store at room temperature. An FBE must also be requested, needs a separate EDTA sample.

Bone marrow aspirate - Sample into  EDTA tube

TissueFresh (if to be shared with other departments for other tests eg. Histopathology or Microbiology) or RPMI fluid (especially if specimen is taken out of hours). This fluid is obtainable from: BH Lab 9895 3884, MH Lab: 9871 3572 or AH Lab: 9764 6136.

CSF/other fluids - at least 2mL is preferred

Collect CSF into three separate, sterile, screw-capped tubes provided (labelled 1,2 & 3)

Tube 1 is for Biochemistry (for Protein and Glucose) 0.5ml (10 drops) required 

Tube 2 for Cytology 1.0ml (20 drops)  and/or Flow Cytometry (6ml)

For Flow Cytometry a minimum of 6ml is required  in Tube 2

For Flow Cytometry and Cytology a minimum of 7ml is required in Tube 2     

Tube 3 for Microbiology upto 10ml (Full tube)

If querying CJD the laboratory requires a 4th unopened tube containing at least 1ml of non-bloodstained CSF.

Xanthochromia investigation requires a 4th tube containing 1ml of CSF .

 

 

Samples must be marked 'URGENT', must reach the laboratory as soon as possible. 

All out-of-hours testing requires notification.

Time and date of collection must be recorded on request form.

Whole blood - 1 x EDTA sample, do not spin. Store at room temperature. An FBE must also be requested,  and needs a separate EDTA sample.

Bone marrow aspirate - Sample into  EDTA tube

TissueFresh (if to be shared with other departments for other tests eg. Histopathology or Microbiology) or RPMI fluid (especially if specimen is taken out of hours). This fluid is obtainable from: BH Lab 9895 3884, MH Lab: 9871 3572 or AH Lab: 9764 6136.

CSF/other fluids - at least 2mL is preferred

Depending on the tests requested, 3-4 separate tubes will need to be provided. Note that the tubes are numbered so that order of collection can be identified – this order should be carefully followed, so that the optimal sample can be used for the requested tests. It is also important to consider the tests that are required, as this will determine how much CSF is needed in each tube. See the matrix below for details.

TUBE 1 is used for Biochemistry and Flow Cytometry. (Centrifuged in the lab – the supernatant is used for Biochemistry analysis (glucose, protein) and the cellular pellet will be used for flow cytometry if required). If tube 3 is bloodstained, take an aliquot for microbiology.

TUBE 2 is used for Cytology

TUBE 3 is for Microbiological analysis (cell count, Gram stain and culture) and any further infectious testing (e.g. viral PCR, mycobacterial PCR and culture, cryptococcal antigen).

TUBE 4 will be required only for;

(a) Creutzfeld-Jacob Disease (CJD) testing OR

(b) Xanthochromia analysis (wrap the tube in foil to prevent sample deterioration due to light).

 

TESTS REQUIRED

MINIMUM REQUIRED VOLUME (mL)

TUBE 1

TUBE 2

TUBE 3

TUBE 4

CHEMISTRY

1.0

 

 

 

ADD FLOW CYTOMETRY

Minimum 5.0-7.0

 

 

 

CYTOLOGY

 

1.0-3.0

 

 

MICROBIOLOGY

 

 

1.0-2.0

 

ADD OTHER INFECTIOUS MARKERS

 

 

add 2.0- 5.0-

 

ADD MYCOBACTERIAL CULTURE

 

 

Add 6.0-10.00

 

ADD CJD

 

 

 

1.0

ADD XANTHOCHROMIA

 

 

 

1.0 (foil)

 

Note, the ability to add further tests to a previously collected sample is dependent upon the volume provided. Also, many tests (flow cytometry, cytology) can only be performed on fresh specimens.

 

Other specialised testing (e.g. oligoclonal bands) will be performed using the supplied samples.

Xanthochromia and CJD analysis cannot be performed on a blood-stained sample

20th July 2021
Assay name

Leucocyte Immunophenotyping

T Cell Subsets

Alternative names

CD4/CD8 Counts, Cell Surface Markers, FACS Analysis, Flow Cytometry

CD2, CD3, CD4, CD8

Preferred Container Type

EDTA
Sterile Jar

EDTA
Lithium heparin no gel

Collection & Request Instructions

Sample store at Room temperature. Samples are not stable for more than 24 hours. Hence they must be received by the Flow Cytometry lab at Box Hill within normal business hours Monday to Friday.

Cutoff for outpatient sample collection on Fridays is at 11.30 AM and MUST reach the laboratory before 4.00PM.

Flow cytometric analysis of cells stained by immunofluorescence with a range of antibodies against lymphoid and myeloid markers.

The panel of markers used will be appropriate to the clinical comments supplied , or as arranged by prior consultation.

The samples are not stable for more than 24 hours. Hence all samples must be recieved in the laboratory at Box Hill  Monday to Friday within business hours.

Cutoff for outpatient sample collection on Fridays is at 11.30 AM and MUST reach the laboratory before 4.00PM.

Processing Instructions

Prefer EDTA sample.

Do not spin, store at room temperature.

Notes

 

 

Samples must be marked 'URGENT', must reach the laboratory as soon as possible. 

All out-of-hours testing requires notification.

Time and date of collection must be recorded on request form.

Whole blood - 1 x EDTA sample, do not spin. Store at room temperature. An FBE must also be requested, needs a separate EDTA sample.

Bone marrow aspirate - Sample into  EDTA tube

TissueFresh (if to be shared with other departments for other tests eg. Histopathology or Microbiology) or RPMI fluid (especially if specimen is taken out of hours). This fluid is obtainable from: BH Lab 9895 3884, MH Lab: 9871 3572 or AH Lab: 9764 6136.

CSF/other fluids - at least 2mL is preferred

Collect CSF into three separate, sterile, screw-capped tubes provided (labelled 1,2 & 3)

Tube 1 is for Biochemistry (for Protein and Glucose) 0.5ml (10 drops) required 

Tube 2 for Cytology 1.0ml (20 drops)  and/or Flow Cytometry (6ml)

For Flow Cytometry a minimum of 6ml is required  in Tube 2

For Flow Cytometry and Cytology a minimum of 7ml is required in Tube 2     

Tube 3 for Microbiology upto 10ml (Full tube)

If querying CJD the laboratory requires a 4th unopened tube containing at least 1ml of non-bloodstained CSF.

Xanthochromia investigation requires a 4th tube containing 1ml of CSF .

Used to monitor T-cell numbers

Preferred EDTA sample. Do not spin, store at room temperature.

19th July 2021
Collection & Request Instructions

Sample store at Room temperature. Samples are not stable for more than 24 hours. Hence they must be recieved by the Flow Cytometry lab at Box Hill within normal business hours Monday to Friday.

Cutoff for outpatirnt sample collection on Fridays is at 12:00 PM and MUST reach the laboratory by 4.00PM.

Flow cytometric analysis of cells stained by immunofluorescence with a range of antibodies against lymphoid and myeloid markers.

The panel of markers used will be appropriate to the clinical comments supplied , or as arranged by prior consultation.

Sample store at Room temperature. Samples are not stable for more than 24 hours. Hence they must be received by the Flow Cytometry lab at Box Hill within normal business hours Monday to Friday.

Cutoff for outpatient sample collection on Fridays is at 11.30 AM and MUST reach the laboratory before 4.00PM.

Flow cytometric analysis of cells stained by immunofluorescence with a range of antibodies against lymphoid and myeloid markers.

The panel of markers used will be appropriate to the clinical comments supplied , or as arranged by prior consultation.

19th July 2021
Collection & Request Instructions

Sample store at Room temperature. Samples are not stable for more than 24 hours. Hence they must be recieved by the Flow Cytometry lab at Box Hill within normal business hours Monday to Friday

Flow cytometric analysis of cells stained by immunofluorescence with a range of antibodies against lymphoid and myeloid markers.

The panel of markers used will be appropriate to the clinical comments supplied , or as arranged by prior consultation.

Sample store at Room temperature. Samples are not stable for more than 24 hours. Hence they must be recieved by the Flow Cytometry lab at Box Hill within normal business hours Monday to Friday.

Cutoff for outpatirnt sample collection on Fridays is at 12:00 PM and MUST reach the laboratory by 4.00PM.

Flow cytometric analysis of cells stained by immunofluorescence with a range of antibodies against lymphoid and myeloid markers.

The panel of markers used will be appropriate to the clinical comments supplied , or as arranged by prior consultation.

Notes

 

 

  • Samples must be marked 'URGENT', must reach the laboratory as soon as possible. 
  • All out-of-hours testing requires notification.
  • Time and date of collection must be recorded on request form.

    Whole blood - 1 x EDTA sample, do not spin. Store at room temperature. An FBE must also be requested, needs a separate EDTA sample.

    Bone marrow aspirate - Sample into  EDTA tube

    TissueFresh (if to be shared with other departments for other tests eg. Histopathology or Microbiology) or RPMI fluid (especially if specimen is taken out of hours). This fluid is obtainable from: BH Lab 9895 3884, MH Lab: 9871 3572 or AH Lab: 9764 6136.

  • CSF/other fluids - at least 2mL is preferred

  • Collect CSF into three separate, sterile, screw-capped tubes provided (labelled 1,2 & 3)

  • Tube 1 is for Biochemistry (for Protein and Glucose) 0.5ml (10 drops) required 
  • Tube 2 for Cytology 1.0ml (20 drops)  and/or Flow Cytometry (6ml)
    • For Flow Cytometry a minimum of 6ml is required  in Tube 2
    • For Flow Cytometry and Cytology a minimum of 7ml is required in Tube 2     
  • Tube 3 for Microbiology upto 10ml (Full tube)

    If querying CJD the laboratory requires a 4th unopened tube containing at least 1ml of non-bloodstained CSF.

    Xanthochromia investigation requires a 4th tube containing 1ml of CSF .

 

 

Samples must be marked 'URGENT', must reach the laboratory as soon as possible. 

All out-of-hours testing requires notification.

Time and date of collection must be recorded on request form.

Whole blood - 1 x EDTA sample, do not spin. Store at room temperature. An FBE must also be requested, needs a separate EDTA sample.

Bone marrow aspirate - Sample into  EDTA tube

TissueFresh (if to be shared with other departments for other tests eg. Histopathology or Microbiology) or RPMI fluid (especially if specimen is taken out of hours). This fluid is obtainable from: BH Lab 9895 3884, MH Lab: 9871 3572 or AH Lab: 9764 6136.

CSF/other fluids - at least 2mL is preferred

Collect CSF into three separate, sterile, screw-capped tubes provided (labelled 1,2 & 3)

Tube 1 is for Biochemistry (for Protein and Glucose) 0.5ml (10 drops) required 

Tube 2 for Cytology 1.0ml (20 drops)  and/or Flow Cytometry (6ml)

For Flow Cytometry a minimum of 6ml is required  in Tube 2

For Flow Cytometry and Cytology a minimum of 7ml is required in Tube 2     

Tube 3 for Microbiology upto 10ml (Full tube)

If querying CJD the laboratory requires a 4th unopened tube containing at least 1ml of non-bloodstained CSF.

Xanthochromia investigation requires a 4th tube containing 1ml of CSF .


 Recent changes for Leucocyte Lysomal Enzyme Analysis

Recent changes for Leucocyte Lysomal Enzyme Analysis

Date
Field
Changed From
Changed To
21st January 2020
Notes

Room temperature.

Please note this test id not covered by Medicare Rebate - see 'Ordering' above.

Room temperature.

Please note this test is not covered by Medicare Rebate - see 'Ordering' above.

21st January 2020
Notes

Room temperature.

This test is not covered by Medicare, cost will be $432.00 as of March 2016.

Room temperature.

Please note this test id not covered by Medicare Rebate - see 'Ordering' above.

21st January 2020
Assay name

White Cell Enzymes

Leucocyte Lysomal Enzyme Analysis

Alternative names

Lysosomal Enzymes

White Cell Enzymes

Volume (Adults)

4 mL

8 mL

Minimum/Paediatric Volume

6 mL

Notes

Room temperature.

This test is not covered by Medicare, cost will be $474.00 as of March 2016.

Room temperature.

This test is not covered by Medicare, cost will be $432.00 as of March 2016.


 Recent changes for Lignocaine

Recent changes for Lignocaine

Date
Field
Changed From
Changed To
8th January 2020
External Laboratory

Pacific Laboratory Medicine Services

Royal Brisbane & Womens Hospital

Frequency

Daily

As required


 Recent changes for Lupus Anticoagulant Screen

Recent changes for Lupus Anticoagulant Screen

Date
Field
Changed From
Changed To
3rd May 2021
External Transport Instructions

Samples can be transported frozen to the laboratory if the facilities are available to double spin and aliquot these sample. This has to be done within 4 hours.

Unspun sample is stable for up to 4 hours and must be transport to laboratory immediately. (Sample must be double spun, aliquoted and frozen within 4 hours from time of collection).

Samples can be transported frozen to the laboratory if the facilities are available to double spin and aliquot these samples. This has to be performed within 4 hours of collection.

Unspun sample is stable for up to 4 hours and must be transport to laboratory immediately. (Sample must be double spun, aliquoted and frozen within 4 hours from time of collection).

3rd May 2021
Collection & Request Instructions

Notification of anticoagulant therapy is essential

One (1x) Sodium citrate tubes is required. Do not use 1 ml citrate tubes.

Filled to label marker and mixed by inversion

The patient must not be receiving heparin

Transport to laboratory immediately. (Sample must be double spun and frozen within 4 hours from time of collection).

Tests performed:

INR, APTT, APTT Mix, Silica Clotting Time and Dilute Russell's Viper venom time (LA screen/Confirm).

 

Notification of anticoagulant therapy is essential

One (1x) Sodium citrate tubes is required. Do not use 1 ml citrate tubes.

Filled to label marker and mixed by inversion

The patient must not be receiving heparin

Unspun sample is stable for up to 4 hours and must be transport to laboratory immediately. (Sample must be double spun, aliquoted and frozen within 4 hours from time of collection).

 

Tests performed:

INR, APTT, APTT Mix, Silica Clotting Time and Dilute Russell's Viper venom time (LA screen/Confirm).

 

Processing Instructions

Transport sample to laboratory within 4 hours of collection.

The sample MUST be double spun and frozen within the 4 hour time limit.

Unspun sample is stable for up to 4 hours and must be transport to laboratory immediately. (Sample must be double spun, aliquoted and frozen within 4 hours from time of collection).

External Transport Instructions

Samples can be transported frozen to the laboratory if the facilities are available to double spin and aliquot these sample. This has to be done within 4 hours.

Unspun sample is stable for up to 4 hours and must be transport to laboratory immediately. (Sample must be double spun, aliquoted and frozen within 4 hours from time of collection).

3rd May 2021
Collection & Request Instructions

Notification of anticoagulant therapy is essential

One (1x) Sodium citrate tubes is required. Do not use 1 ml citrate tubes.

Filled to label marker and mixed by inversion

The patient must not be receiving heparin

Transport to laboratory immediately. (maximum of 4 hours from time of collection).

Tests performed:

INR, APTT, APTT Mix, Silica Clotting Time and Dilute Russell's Viper venom time (LA screen/Confirm).

 

Notification of anticoagulant therapy is essential

One (1x) Sodium citrate tubes is required. Do not use 1 ml citrate tubes.

Filled to label marker and mixed by inversion

The patient must not be receiving heparin

Transport to laboratory immediately. (Sample must be double spun and frozen within 4 hours from time of collection).

Tests performed:

INR, APTT, APTT Mix, Silica Clotting Time and Dilute Russell's Viper venom time (LA screen/Confirm).

 

Processing Instructions

Transport sample to laboratory within 4 hours of collection.

The sample MUST be double spun and frozen within the 4 hour time limit.

19th January 2021
Method

Centrifuge the two 3.5 mL citrate tubes and transfer the plasma to a labeled 4 mL plastic tube and re-centrifuge at room temperature (do not use the refrigerated centrifuge).

The double centrifuged plasma is transferred to two 1 mL eppendorf tubes and freeze.

Sample must be centrifuged within 4 hours of collection.

**Sample must be centrifuged within 4 hours of collection**

Centrifuge the two 3.5 mL citrate tubes (and transfer the plasma to a labeled 4 mL plastic tube and re-centrifuge at room temperature (do not use the refrigerated centrifuge).  One sample is acceptable but 2 samples are preferable for Lupus testing.

The double centrifuged plasma is transferred into a new labelled tubes. 

Double spun samples are then frozen in the -20'C freezer.

19th January 2021
Collection & Request Instructions

Notification of anticoagulant therapy is essential

One (1x) Sodium citrate tubes is required. Do not use 1 ml citrate tubes.

Filled to label marker and mixed by inversion

The patient must not be receiving heparin

Transport to laboratory immediately.

Tests performed:

INR, APTT, APTT Mix, Lupus sensitive APTT and Dilute Russell's Viper venom time (LA screen/Confirm).

 

Notification of anticoagulant therapy is essential

One (1x) Sodium citrate tubes is required. Do not use 1 ml citrate tubes.

Filled to label marker and mixed by inversion

The patient must not be receiving heparin

Transport to laboratory immediately. (maximum of 4 hours from time of collection).

Tests performed:

INR, APTT, APTT Mix, Silica Clotting Time and Dilute Russell's Viper venom time (LA screen/Confirm).

 

Method

Centrifuge the two 3.5 mL citrate tubes and transfer the plasma to a labeled 4 mL plastic tube and re-centrifuge at room temperature (do not use the refrigerated centrifuge).

The double centrifuged plasma is transferred to two 1 mL eppendorf tubes and freeze.

Centrifuge the two 3.5 mL citrate tubes and transfer the plasma to a labeled 4 mL plastic tube and re-centrifuge at room temperature (do not use the refrigerated centrifuge).

The double centrifuged plasma is transferred to two 1 mL eppendorf tubes and freeze.

Sample must be centrifuged within 4 hours of collection.

10th December 2020
Collection & Request Instructions

Notification of anticoagulant therapy is essential

Two (2x) Sodium citrate tubes required. Do not use 1 ml citrate tubes.

Filled to label marker and mixed by inversion

The patient must not be receiving heparin

Transport to laboratory immediately.

Tests performed:

INR, APTT, APTT Mix, Lupus sensitive APTT and Dilute Russell's Viper venom time (LA screen/Confirm).

 

Notification of anticoagulant therapy is essential

One (1x) Sodium citrate tubes is required. Do not use 1 ml citrate tubes.

Filled to label marker and mixed by inversion

The patient must not be receiving heparin

Transport to laboratory immediately.

Tests performed:

INR, APTT, APTT Mix, Lupus sensitive APTT and Dilute Russell's Viper venom time (LA screen/Confirm).

 


 Recent changes for Lymphocyte subsets

Recent changes for Lymphocyte subsets

Date
Field
Changed From
Changed To
14th October 2021
Alternative names

CD3 count, CD4 count, CD8 count, CD4:CD8 ratio CD19 count, CD56 count, flow cytometry

14th October 2021
Alternative names

CD19 count & CD56 count, CD4 count, CD4 count, CD4:CD8 ratio Lymphocyte subsets include: CD3 count, CD4:CD8 ratio, CD8 count, CD8 count, T subsets include: CD3 count

T subsets include: CD3 count, CD4 count, CD8 count, CD4:CD8 ratio Lymphocyte subsets include: CD3 count, CD4 count, CD8 count, CD4:CD8 ratio, CD19 count & CD56 count Flow Cytometry

Collection & Request Instructions

Sample store at Room temperature. Samples are not stable for more than 24 hours. Hence they must be received by the Flow Cytometry lab at Box Hill within normal business hours Monday to Friday.

Cut-off for outpatient sample collection on Fridays is at 11.30 AM and MUST reach the laboratory before 4.00PM.

Flow cytometric analysis of cells stained by immunofluorescence with a range of antibodies against lymphoid and myeloid markers.

The panel of markers used will be appropriate to the panel selected and the clinical comments supplied, or as arranged by prior consultation.

Sample store at ROOM TEMPERATURE. Samples are not stable for more than 24 hours. Hence they must be received by the Flow Cytometry lab at Box Hill within normal business hours Monday to Friday.

Cut-off for outpatient sample collection on Fridays is at 11.30 AM and MUST reach the laboratory before 4.00PM.

Flow cytometric analysis of cells stained by immunofluorescence with a range of antibodies against lymphoid and myeloid markers.

The panel of markers used will be appropriate to the panel selected and the clinical comments supplied, or as arranged by prior consultation.

14th October 2021
Collection & Request Instructions

The samples are not stable for more than 24 hours. Hence all samples must be recieved in the laboratory at Box Hill  Monday to Friday within business hours.

Cutoff for outpatient sample collection on Fridays is at 11.30 AM and MUST reach the laboratory before 4.00PM.

 

Sample store at Room temperature. Samples are not stable for more than 24 hours. Hence they must be received by the Flow Cytometry lab at Box Hill within normal business hours Monday to Friday.

Cut-off for outpatient sample collection on Fridays is at 11.30 AM and MUST reach the laboratory before 4.00PM.

Flow cytometric analysis of cells stained by immunofluorescence with a range of antibodies against lymphoid and myeloid markers.

The panel of markers used will be appropriate to the panel selected and the clinical comments supplied, or as arranged by prior consultation.

Frequency

Daily

Notes

Used to monitor T-cell numbers

Preferred EDTA sample. Do not spin, store at room temperature.

Samples must be marked 'URGENT', must reach the laboratory as soon as possible. 

All out-of-hours testing requires notification.

Time and date of collection must be recorded on request form.

Whole blood - 1 x EDTA sample, do not spin. Store at room temperature. An FBE must also be requested, and needs a separate EDTA sample.

14th October 2021
Assay name

T Cell Subsets

Lymphocyte subsets

Alternative names

CD2, CD3, CD4, CD8

T subsets include: CD3 count, CD4 count, CD8 count, CD4:CD8 ratio Lymphocyte subsets include: CD3 count, CD4 count, CD8 count, CD4:CD8 ratio, CD19 count & CD56 count

Request Group

FLOW

TSUB & LSUB

20th July 2021
Processing Instructions

Prefer EDTA sample.

Do not spin, store at room temperature.

Prefer EDTA sample.

Do not spin, store at room temperature.

20th July 2021
Notes

Used to monitor T-cell numbers

Preferred EDTA sample. Do not spin, store at room temperature.

Used to monitor T-cell numbers

Preferred EDTA sample. Do not spin, store at room temperature.

20th July 2021
Collection & Request Instructions

The samples are not stable for more than 24 hours. Hence all samples must be recieved in the laboratory at Box Hill  Monday to Friday within business hours.

The samples are not stable for more than 24 hours. Hence all samples must be recieved in the laboratory at Box Hill  Monday to Friday within business hours.

Cutoff for outpatient sample collection on Fridays is at 11.30 AM and MUST reach the laboratory before 4.00PM.

 

Processing Instructions

Prefer EDTA sample.

Do not spin, store at room temperature.

Prefer EDTA sample.

Do not spin, store at room temperature.

Notes

Used to monitor T-cell numbers

Preferred EDTA sample. Do not spin, store at room temperature.

Used to monitor T-cell numbers

Preferred EDTA sample. Do not spin, store at room temperature.


 Recent changes for Mumps Virus PCR

Recent changes for Mumps Virus PCR

Date
Field
Changed From
Changed To
3rd January 2020
Preferred Container Type

Swab - red top, plain swab, wooden shaft
Sterile Jar

Swab - red top, plain swab, wooden shaft
Swab - blue top (Amies transport media)
Swab - white top, plain swab, plastic shaft
UTM/Sterile pot


 Recent changes for Mycobacterium ulcerans PCR

Recent changes for Mycobacterium ulcerans PCR

Date
Field
Changed From
Changed To
3rd March 2021
Alternative names

Mycobacterium ulcerans NAD

Mycobacterium ulcerans NAD, Buruli ulcer, Bairnsdale ulcer

Collection & Request Instructions

 

Specimen Required: Fresh tissue biopsy, dry swabs and paraffin-embedded fixed tissue sections (at least 6 sections, each 10-20 mm thick)

Specimen Required: Fresh tissue biopsy, dry swabs (collect x2 - one for Mycobacterial culture and one for M.ulcerans PCR) and paraffin-embedded fixed tissue sections (at least 6 sections, each 10-20 mm thick). A gel swab may be used if unable to obtain a dry swab.


 Recent changes for Paroxysmal Nocturnal Haemoglobinuria

Recent changes for Paroxysmal Nocturnal Haemoglobinuria

Date
Field
Changed From
Changed To
15th October 2021
Collection & Request Instructions

Separate specimen required.

Cutoff for outpatient sample collection on Fridays is at 11.30 AM and MUST reach the laboratory before 4.00PM.

Whole blood - 1 x EDTA sample, do not spin. Store at room temperature. An FBE and BLOOD FILM must also be requested,  and needs a separate EDTA sample.

Cutoff for outpatient sample collection on Fridays is at 11.30 AM and MUST reach the laboratory before 4.00PM.

12th October 2021
Alternative names

CD 59, PNH flow cytometry, PNH screen

CD59, PNH screen flow cytometry, FLEAR

12th October 2021
Alternative names

CD 59, PNH screen

CD 59, PNH screen, PNH flow cytometry

Request Group

FLOW

PNHS

20th July 2021
Collection & Request Instructions

Separate specimen required.

Separate specimen required.

Cutoff for outpatient sample collection on Fridays is at 11.30 AM and MUST reach the laboratory before 4.00PM.


 Recent changes for Plasminogen

Recent changes for Plasminogen

Date
Field
Changed From
Changed To
21st December 2020
Volume (Adults)

4 mL

3.5 mL

17th December 2020
External Laboratory

Austin Pathology

Monash Pathology


 Recent changes for Protein C

Recent changes for Protein C

Date
Field
Changed From
Changed To
19th January 2021
Collection & Request Instructions

Transport specimen at Room Temperature to lab

Collection & Request Instructions

Fill to label marker and mix by inversion.

Do not use 1 mL citrate tubes.

1 x 3.5 ml citrate tubes sufficient for Protein C, Protein S and Antithrombin 3.

Fill to label marker and mix by inversion.

Do not use 1 mL citrate tubes.

Samples must be transported to laboratory immediately. ( Within a maximun of 4 hours from collection time)

1 x 3.5 ml citrate tubes sufficient for Protein C, Protein S and Antithrombin 3.

10th December 2020
Volume (Adults)

3.5 mL

10th December 2020
Collection & Request Instructions

Fill to label marker and mix by inversion.

Do not use 1 mL citrate tubes.

2 x 3.5 ml citrate tubes sufficient for Protein C, Protein S and Antithrombin 3.

Fill to label marker and mix by inversion.

Do not use 1 mL citrate tubes.

1 x 3.5 ml citrate tubes sufficient for Protein C, Protein S and Antithrombin 3.

Notes

Results not valid if patient receiving warfarin therapy or with acute thrombotic event.

Warfarin falsely lowers Protein C levels.

Fill to label marker and mix by inversion.

Do not use 1 mL citrate tubes.

2 x 3.5 ml citrate sufficient for Protein C, Protein S and Antithrombin 3.

Results not valid if patient receiving warfarin therapy or with acute thrombotic event.

Warfarin falsely lowers Protein C levels.

Fill to label marker and mix by inversion.

Do not use 1 mL citrate tubes.

1 x 3.5 ml citrate sufficient for Protein C, Protein S and Antithrombin 3.


 Recent changes for Protein S

Recent changes for Protein S

Date
Field
Changed From
Changed To
19th January 2021
Collection & Request Instructions

Fill to label marker and mix by inversion

Do not use 1 ml citrate tubes.

1 x 3.5 mL citrate tubes sufficient for Protein C, Protein S and Antithrombin 3.

Fill to label marker and mix by inversion

Do not use 1 ml citrate tubes.

Samples must be transported to laboratory immediately. ( Within a maximun of 4 hours from collection time)

1 x 3.5 mL citrate tubes sufficient for Protein C, Protein S and Antithrombin 3.

10th December 2020
Volume (Adults)

7 mL

3.5 mL

10th December 2020
Collection & Request Instructions

Fill to label marker and mix by inversion

Do not use 1 ml citrate tubes.

2 x 3.5 mL citrate tubes sufficient for Protein C, Protein S and Antithrombin 3.

Fill to label marker and mix by inversion

Do not use 1 ml citrate tubes.

1 x 3.5 mL citrate tubes sufficient for Protein C, Protein S and Antithrombin 3.

Notes

Results not valid if patient receiving warfarin therapy or with acute thrombotic event

Warfarin falsely lowers Protein S level.

Fill to label marker and mix by inversion

Do not use 1 ml citrate tubes.

2 x 3.5 mL citrate tubes sufficient for Protein C, Protein S and Antithrombin 3.

Results not valid if patient receiving warfarin therapy or with acute thrombotic event

Warfarin falsely lowers Protein S level.

Fill to label marker and mix by inversion

Do not use 1 ml citrate tubes.

1 x 3.5 mL citrate tubes sufficient for Protein C, Protein S and Antithrombin 3.


 Recent changes for Pseudocholinesterase

Recent changes for Pseudocholinesterase

Date
Field
Changed From
Changed To
7th February 2020
Assay name

Cholinesterase

Pseudocholinesterase

Collection & Request Instructions

Please request serum BCHE activity with the Genetic test.

Genetic testing sample : EDTA  whole blood 5mL minimum

 

 

 

Please request serum BCHE activity with the Genetic test.

Genetic testing sample : EDTA  whole blood 5mL minimum  and serum for BCHE activity.

 

 

 

30th January 2020
Alternative names

Pseudocholinesterase

Pseudocholinesterase, Butyrylcholinesterase

Preferred Container Type

Pink EDTA (6mL)

Pink EDTA (6mL)
Serum tube with gel

Collection & Request Instructions

Genetic testing sample : EDTA  whole blood 5mL minimum

 

Please request serum BCHE activity with the Genetic test.

Genetic testing sample : EDTA  whole blood 5mL minimum

 

 

 

Processing Instructions

Genetic testing on unspun whole blood is performed by:

Molecular Genetics Department, Pathwest

QE II Medical centre,

Hospital Ave, Nedlands WA.  6009

Phone Lab Results 08 6383 4223  Spec Recepn. 08 6383 4956

Enzyme testing (serum) : RPA (Sydney) Samples can be frozen, Batched & sent weekly

Genetic testing and enzyme testing: please send samples together packed with ice pack.

Please send patient financial consent form. Cost of the test: $150.

External Laboratory

Pathwest Lab Med WA

Sydney South West Pathology Service(RPA)


 Recent changes for Renin - plasma

Recent changes for Renin - plasma

Date
Field
Changed From
Changed To
3rd December 2019
Collection & Request Instructions

 

Collection Requirements for both or either tests

Collect into 4 mL EDTA tubes at Room Temperature. Do not collect on ice

If both Renin and Aldosterone are requested together, they can be collected into the same EDTA tube

In the laboratory, spin and separate the plasma immediately. Plasma is stable at Room Temperature if analysis is performed on the same day.

Renin must be frozen at -80 C. Transport plasma at room temperature until it can be place in -80 C Freezer.(Must be done within 3-4).

Add-on tests for Renin and Aldosterone should not be done on samples which have been previously collected or stored at 4 C.

Renin will not be collected at Healesville Hospital and Yarra Ranges Health Collection Rooms.

 

Collection Requirements for both or either tests

Collect into 4 mL EDTA tubes at Room Temperature. Do not collect on ice

If both Renin and Aldosterone are requested together, they can be collected into the same EDTA tube

In the laboratory, spin and separate the plasma immediately. Plasma is stable at Room Temperature if analysis is performed on the same day.

Renin must be frozen at -80 C. Transport plasma at room temperature (Maroondah and Angliss) until it can be place in -80 C Freezer.(Must be done within 3-4 hors).

Add-on tests for Renin and Aldosterone should not be done on samples which have been previously collected or stored at 4 C.

Renin will not be collected at Healesville Hospital and Yarra Ranges Health Collection Rooms.

3rd December 2019
Collection & Request Instructions

 

Collection Requirements for both or either tests

  • Collect into 4 mL EDTA tubes at Room Temperature. Do not collect on ice
  • If both Renin and Aldosterone are requested together, they can be collected into the same EDTA tube
  • In the laboratory, spin and separate the plasma immediately. Plasma is stable at Room Temperature if analysis is performed on the same day
  • If the specimen arrives on the weekend or after hours, or if the specimen is referred from an external laboratory, freeze the plasma
  • EDTA plasma samples from external laboratories should be stored and transported frozen
  • Whole blood or plasma stored at 4 Deg C is not suitable
  • Add-on tests for Renin and Aldosterone should not be done on samples which have been previously collected or stored at 4 Deg C

 

Collection Requirements for both or either tests

Collect into 4 mL EDTA tubes at Room Temperature. Do not collect on ice

If both Renin and Aldosterone are requested together, they can be collected into the same EDTA tube

In the laboratory, spin and separate the plasma immediately. Plasma is stable at Room Temperature if analysis is performed on the same day.

Renin must be frozen at -80 C. Transport plasma at room temperature until it can be place in -80 C Freezer.(Must be done within 3-4).

Add-on tests for Renin and Aldosterone should not be done on samples which have been previously collected or stored at 4 C.

Renin will not be collected at Healesville Hospital and Yarra Ranges Health Collection Rooms.

Processing Instructions

Spin,seperate and freeze, transport frozen.

Minimum plasma volume: 500 ul (1mL if aldosterone is also requested)

Spin,seperate and freeze (-80 C), transport frozen.

Minimum plasma volume: 500 ul (1mL if aldosterone is also requested)

Notes

Prior to testing, patients should be withdrawn from diuretics, ACE inhibitors, angiotensin II receptor antagonists, beta blockers and calcium channel blockers for 3 weeks. Patients should also be well hydrated, have had 7g/day of salt for one week and have normal plasma potassium and bicarbonate levels.

  •  Deliver to laboratory ASAP at room temperature

Prior to testing, patients should be withdrawn from diuretics, ACE inhibitors, angiotensin II receptor antagonists, beta blockers and calcium channel blockers for 3 weeks. Patients should also be well hydrated, have had 7g/day of salt for one week and have normal plasma potassium and bicarbonate levels.

 Deliver to laboratory ASAP at room temperature


 Recent changes for Risperidone

Recent changes for Risperidone

Date
Field
Changed From
Changed To
18th December 2020
External Laboratory

Pathwest Lab Med WA

Alfred Hospital


 Recent changes for Ristocetin Co-Factor assay

Recent changes for Ristocetin Co-Factor assay

Date
Field
Changed From
Changed To
11th June 2021
Alternative names

RICOF

RICOF, vWF:Activity, vWF:Act

Laboratory

Referred Test

Coagulation

External Laboratory

Austin Pathology


 Recent changes for Scabies investigation

Recent changes for Scabies investigation

Date
Field
Changed From
Changed To
8th June 2021
Collection & Request Instructions

Scabies collection instructions

Preparation

Wash the area of skin to be scraped with an alcohol swab.

Remove any ointment or residual applications using soap and water.

Disinfect the area with a skin cleansing alcohol wipe and allow it to dry.

Abrade the infested area with a sterile scalpel to the depth of pinpoint bleeding.

Transfer skin scrapings to a sterile yellow top pot for transport to the laboratory. Do NOT include the scalpel – dispose of in sharps bin

Label pot with patient name, UR No and DOB, date of collection, site of collection

Preparation

Wash the area of skin to be scraped with an alcohol swab.

Remove any ointment or residual applications using soap and water.

Disinfect the area with a skin cleansing alcohol wipe and allow it to dry.

Abrade the infested area with a sterile scalpel to the depth of pinpoint bleeding.

Transfer skin scrapings to a sterile yellow top pot for transport to the laboratory. Do NOT include the scalpel – dispose of in sharps bin

Label pot with patient name, UR No and DOB, date of collection, site of collection


 Recent changes for Test

Recent changes for Test

Date
Field
Changed From
Changed To
3rd December 2020
Method

Laboratory notes testing

Laboratory notes - email test


 Recent changes for Thrombophilia Screen

Recent changes for Thrombophilia Screen

Date
Field
Changed From
Changed To
10th December 2020
Collection & Request Instructions

For full thrombophilia screen including INR, APTT, Protein C, Protein S, AT3, LA screen, APCR, Homocystine, Factor V Leiden, PG20210A and ACA:-

5 x 3.5 ml citrate tubes plus 2 EDTA tubes and 1 x serum tube will be required.

For full thrombophilia screen including INR, APTT, Protein C, Protein S, AT3, LA screen, , Homocystine, Factor V Leiden, PG20210A and ACA/ACL antibodies:-

3 x 3.5 ml citrate tubes plus 2 EDTA tubes and 1 x serum tube will be required.

Notes

May include Protein C functional, Protein S free, Antithrombin III, APCR ratio, Factor V Leiden, Homocystine, Anti-cardiolipin antibodies, Lupus Anticoagulant, Prothrombin mutation.

Which tests are most appropriate depends on clinical history

Tests need to be listed individually to comply with HIC guidelines

Consult Haematology Registrar or Haematologist.

For full thrombophilia screen including INR, APTT, Protein C, Protein S, AT3, LA screen, APCR, Homocystine, Factor V Leiden, PG20210A and ACA:- 5 x 3.5 ml citrate tubes plus 2 EDTA tubes and 1 x serum tube will be required.

May include Protein C functional, Protein S free, Antithrombin III,  Factor V Leiden, Homocystine, Anti-cardiolipin antibodies, Lupus Anticoagulant, Prothrombin Gene Mutation.

Which tests are most appropriate depends on clinical history

Tests need to be listed individually to comply with HIC guidelines

Consult Haematology Registrar or Haematologist.

For full thrombophilia screen including INR, APTT, Protein C, Protein S, AT3, LA screen, Homocystine, Factor V Leiden, PG20210A and ACA:- 3 x 3.5 ml citrate tubes plus 2 EDTA tubes and 1 x serum tube will be required.


 Recent changes for Urine Nickel

Recent changes for Urine Nickel

Date
Field
Changed From
Changed To
17th January 2020
External Laboratory

Sydney South West Pathology Service(RPA)

Alfred Hospital

Frequency

As required


 Recent changes for Xanthochromia

Recent changes for Xanthochromia

Date
Field
Changed From
Changed To
14th July 2021
Laboratory

Referred Test

Biochemistry

Collection & Request Instructions

Please note : to avoid sample deterioration, DO NOT send CSF specimens via the vacuum chute system.

Please quickly check that the tube is labelled underneath the foil before referring.

THERE MUST BE A MINIMUM OF 0.6 mL OF CSF OR THE TEST CANNOT BE PERFORMED

 

Please note : to avoid sample deterioration, DO NOT send CSF specimens via the vacuum chute system.

 

THERE MUST BE A MINIMUM OF 0.6 mL OF CSF OR THE TEST CANNOT BE PERFORMED

 

Processing Instructions

CSF xanthochromia checklist

Sample collected >12hrs from onset symptoms   Preferable

 

      4th CSF sample    Essential

 

Sample light protected immediately   Essential

 

Sample transported manually to lab   Essential

 

Serum sample for bilirubin & albumin   Preferable

 

Slip with clinical notes and contact details   Essential

 

CSF xanthochromia checklist

Sample collected >12hrs from onset symptoms   Preferable

 

      4th CSF sample    Essential

 

Sample light protected immediately   Essential

 

Sample transported manually to lab   Essential

 

Serum sample for bilirubin & protein- essemtial

 

Slip with clinical notes and contact details   Essential

 

External Laboratory

Austin Pathology

External Transport Instructions

Send via a Taxi ASAP. Ring Austin Pathology on 94963100.

MH and AH send via taxi ASAP and notify BH laboraotry.

Frequency

Daily

As required

Method

Please quickly check that the tube is labelled underneath the foil before passing to Scientist.

The specimen designated for spectrophotometry should be centrifuged at .2000 rpm for 5 min as soon as possible after receipt in the laboratory. The supernatant must be stored in the dark at 40 C until analysis.

Notes

This test is specialised and will only be performed on CSF's when:

CT scan is negative

 

Four tubes must be collected and the container of the 4th tube must be wrapped in foil (please Label specimen before wrapping in foil)

This test is specialised and will only be performed on CSF's when:

CT scan is negative

Four tubes must be collected and the container of the 4th tube must be wrapped in foil (please Label specimen before wrapping in foil)

A white top tube must be collected for simulataneous measurement of serum Bilirubin and serum Protein (or add on to existing bloods within 6 hours of CSF collection).