Pathology

Date

Field

Changed From

Changed To

Notes

Consultation with Endocrinologist suggested

Collect in the morning between 0800 and 1000h.

1 x 4mL EDTA tube on ice.

Send to laboratory for immediate centrifugation. Spin, separate and freeze plasma. Transport frozen.

Consultation with Endocrinologist suggested

Collect 1 x 4mL EDTA tube on ice.

Send to laboratory for immediate centrifugation. Spin, separate and freeze plasma. 

Date

Field

Changed From

Changed To

Laboratory

Referred Test

Biochemistry

Collection & Request Instructions

Collection Requirements for both or either tests

Collect into 4 mL EDTA tubes at Room Temperature. Do not collect on ice

If both Renin and Aldosterone are requested together, they can be collected into the same EDTA tube

In the laboratory, spin and separate the plasma immediately. Plasma is stable at Room Temperature if analysis is performed on the same day

If the specimen arrives on the weekend or after hours, or if the specimen is referred from an external laboratory, freeze the plasma

EDTA plasma samples from external laboratories should be stored and transported frozen

Whole blood or plasma stored at 4 Deg C is not suitable

Add-on tests for Renin and Aldosterone should not be done on samples which have been previously collected or stored at 4 Deg C

Collection Requirements for both or either tests

Collect into 4 mL EDTA tubes at Room Temperature. Do not collect on ice

If both Renin and Aldosterone are requested together, they can be collected into the same EDTA tube

In the laboratory, spin and separate the plasma immediately. Plasma is stable at Room Temperature if analysis is performed on the same day

If the specimen arrives on the weekend or after hours freeze the plasma

EDTA plasma samples from external laboratories should be stored and transported frozen

Whole blood or plasma stored at 4 Deg C is not suitable

Add-on tests for Renin and Aldosterone should not be done on samples which have been previously collected or stored at 4 Deg C

External Laboratory

Austin Pathology

Date

Field

Changed From

Changed To

External Laboratory

St Vincents Hospital

Date

Field

Changed From

Changed To

External Laboratory

Pathwest Lab Med WA

Date

Field

Changed From

Changed To

External Laboratory

Pathwest Lab Med WA

Date

Field

Changed From

Changed To

Frequency

Twice weekly

Weekly

Collection & Request Instructions

Immunodiffusion for Aspergillus fumigatus antibodies in allergic Aspergillosis.

Test for Aspergillus fumigatus antibodies.

External Laboratory

Monash Pathology

Method

Test performed by Royal North Shore Hospital, NSW

Sent with ICPMR courier

Date

Field

Changed From

Changed To

Processing Instructions

Place the samples and request slip in fridge (M43) in CSR. Do not enter in the computer.

Eastern Health Clinical School
Level 2, 5 Arnold Street,
Box Hill  Vic  3128
Ph: 61 3 9091 8827
Fax: 61 3 9899 9137
E-mail:  [email protected]

Date

Field

Changed From

Changed To

Notes

Transport specimen at Room Temperature to lab
Sample haemolysis may make this test unreportable

Transport specimen at Room Temperature to lab
Sample haemolysis may make this test unreportable

Send as whole blood

Date

Field

Changed From

Changed To

External Laboratory

Austin Pathology

Date

Field

Changed From

Changed To

Notes

LDL was previously calculated from triglyceride, cholesterol and HDL cholesterol using the Friedewald formula.

As from 22nd of February 2024, LDL is calculated using the Sampson equation.
Friedewald knew that with increasing triglyceride levels, the calculated LDL-C was progressively falsely lower when compared with the gold standard technique and therefore Friedewald stated LDL-C should not be calculated when triglycerides are > 4.5 mmol/L. With much lower LDL-C levels seen in the statin era, an improved calculation was required, hence the Sampson equation.

Comparing both equations with the gold standard ultracentrifugation method, Sampson LDL-C offers similar or better accuracy than Friedewald LDL-C at any triglyceride level. Sampson LDL-C can also be calculated up to a triglyceride level of 9 mmol/L.

Friedewald: LDL-C = TC – HDL-C – Triglycerides/2.2

Sampson: LDL-C=TC/0.948−HDL-C/0.971−(TG/8.56+TG × non-HDL-C/2140 −TG2/16100)−9.44

The calculation for the Sampson equation is complicated but Friedewald LDL-C can be (easily) calculated from the available lipid results if required.
 

Date

Field

Changed From

Changed To

Notes

IgG and IgM, IgG Avidity Index performed

IgG and IgM, IgG Avidity Index performed if IgM is positive

Laboratory

Microbiology

Biochemistry

Date

Field

Changed From

Changed To

Collection & Request Instructions

Pre heated 6 mL EDTA tube. Tubes,needles and syringes must be pre-warmed at 37^oC and specimen placed into a hot box (obtained from the laboratory) and transported to the laboratory immediately. Cold specimens may give erroneous results.

Collect a Hot Box with 2x pre-heated, 6 mL EDTA tube from Pathology Specimen Reception. Sample collection must occur within 10min. Once samples are collected, transport to the laboratory immediately.

Cold specimens may give erroneous results.

Processing Instructions

Specimen must be allowed to settle in 37°C incubator (tubes must NOT be allowed to cool to RT prior to separation). Once seperated, can be stored according to laboratory protocols.

Specimens must be handed to Blood Bank Scientist immediately to place in their waterbath. Please do not leave in the rack.

External Transport Instructions

Once plasma is separated, transportation and storage can be at 4°C.

Angliss and Maroondah lab: Once plasma is separated, transportation and storage can be at 4°C.

Frequency

Daily

Mon-Fri

Notes

Investigation of cold agglutinin specificity, thermal range and titre by consultation with Haematologist

Tubes warmed to 37^oC are available from Blood Bank laboratory, Box Hill.

Test performed during business hours.

Test performed during business hours, Monday to Friday.

Minimum/Paediatric Volume

N/A mL

Request Group

CAGS

CAGG

Laboratory

Referred Test

Blood Bank

Volume (Adults)

5 mL

Preferred Container Type

Serum No Gel & EDTA

Pink EDTA (6mL)

Collection & Request Instructions

Pre heated 4 mL EDTA tube and 10 mL plain tube. Tubes,needles and syringes must be pre-warmed at 37^oC and specimen placed into a thermos flask ( obtained from the laboratory) and transported to the laboratory immediately. Cold specimens may give erroneous results.

Pre heated 6 mL EDTA tube. Tubes,needles and syringes must be pre-warmed at 37^oC and specimen placed into a hot box (obtained from the laboratory) and transported to the laboratory immediately. Cold specimens may give erroneous results.

Processing Instructions

Specimen must be centrifuged and separated immediately upon receipt (tubes must NOT be allowed to cool to RT prior to separation)

Specimen must be allowed to settle in 37°C incubator (tubes must NOT be allowed to cool to RT prior to separation). Once seperated, can be stored according to laboratory protocols.

External Laboratory

Austin Pathology

External Transport Instructions

Once plasma is separated, transportation and storage can be at 4 degrees.

Once plasma is separated, transportation and storage can be at 4°C.

Frequency

As required

Daily

Notes

Investigation of cold agglutinin specificity, thermal range and titre by consultation with Haematologist

Tubes warmed to 37^oC are available from the Core laboratory

Investigation of cold agglutinin specificity, thermal range and titre by consultation with Haematologist

Tubes warmed to 37^oC are available from Blood Bank laboratory, Box Hill.

Test performed during business hours.

Date

Field

Changed From

Changed To

Collection & Request Instructions

Transport ON ICE IMMEDIATELY to lab

Transport specimen at Room Temperature to lab

Date

Field

Changed From

Changed To

Collection & Request Instructions

Requests for Alzheimer's disease - CSF must be collected in a seperate blue capped PP tube, available from Pathology Specimen Reception (CSR).

Processing Instructions

DO NOT CENTRIFUGE SPECIMEN

This test requires authorisation by the Neurology Registrar or Neurology Consultant.

CSF Specimens are unsuitable for testing if macroscopically haemorrhagic, xanthochromic, have red blood cell counts < 500/uL, have white cell counts < 10/uL

DO NOT CENTRIFUGE SPECIMEN

This test requires authorisation by the Neurology Registrar or Neurology Consultant.

CSF Specimens are unsuitable for testing if macroscopically haemorrhagic, xanthochromic. Cell count must have red blood cell counts < 500/uL, and white cell counts < 10/uL

Notes

This test requires authorisation by the Neurology Registrar or Neurology Consultant.

In addition, please refer to Eastern Health Infection Control CJD procedure and contact Infection Control prior to performing the Lumbar Puncture

Test is run weekly. Samples received by 4 p.m. Friday will have the result issued by the end of the following week.

CSF : Tests for 14-3-3 CSF protein

At least 1 mL of CSF is required in a separate (4th), unopened tube.

Double bag specimen. Must be frozen and transported on dry ice.

Blood : Test for PRNP (Prion protein gene)

5 mLs in EDTA tube

This test requires authorisation by the Neurology Registrar or Neurology Consultant.

In addition, please refer to Eastern Health Infection Control CJD procedure and contact Infection Control prior to performing the Lumbar Puncture

Test is run weekly. Samples received by 4 p.m. Friday will have the result issued by the end of the following week.

CSF : Tests for 14-3-3 CSF protein

At least 1 mL of CSF is required in a separate (4th), unopened tube.

Double bag specimen. Note: sample is no longer required to be sent frozen.

Blood : Test for PRNP (Prion protein gene)

5 mLs in EDTA tube

Date

Field

Changed From

Changed To

Collection & Request Instructions

This test required approval from the Clinical Microbiologist. Minimum 200uL/0.2mL of unspun CSF is required for this test.

This test required approval from the Clinical Microbiologist. Minimum 200uL/0.2mL of unspun CSF is required for this test.

CSF collected via lumbar punture only; bloodstained/xanthochromic samples are suitable

Notes

NOTE: Non-K1 E. coli serotypes may be present in a specimen and will not be detected by the BioFire ME Panel.
NOTE: Non-encapsulated strains of Neisseria meningitidis are not detected by the BioFire ME Panel.
NOTE: The BioFire ME Panel does not distinguish between latent and active CMV and HHV-6 infections. Detection of these viruses may indicate primary infection, secondary reactivation, or the presence of latent virus. Results should always be interpreted in conjunction with other clinical, laboratory, and epidemiological information.
NOTE: Patients with a suspicion of cryptococcal meningitis and a negative cryptococcal PCR result, such as by the BioFire ME Panel, should be tested for CrAG.

Date

Field

Changed From

Changed To

Alternative names

D Dimer, DDimer, Fibrin breakdown products

D Dimer, DDimer

Date

Field

Changed From

Changed To

Laboratory

Microbiology

Biochemistry

Date

Field

Changed From

Changed To

Volume (Adults)

1.5 mL

1 mL

Minimum/Paediatric Volume

0.5 mL

Preferred Container Type

ESR tube

EDTA
EDTA - Paediatric

Collection & Request Instructions

Using the ESR tube :
Transport specimen at Room Temperature to lab
Sample haemolysis may make this test unreportable

Transport specimen at Room Temperature to lab
Sample haemolysis may make this test unreportable
Samples found to have clots cannot be tested

Collection & Request Instructions

This tube consists of an inner vessel with an outer sleeve.  The outer sleeve MUST remain in place as this is where sample identification (label or handwritten) will be placed. 

Frequency

Daily

Notes

If the ESR test is requested in conjunction with a FBE test request, a single EDTA tube may be shared. In these instances;

Minimum Adult Volume: 1.5 mL

Minimum Paediatric Volume: 1 mL

Date

Field

Changed From

Changed To

Collection & Request Instructions

For non-rebatable tests the out of pocket cost is $60

Sample must be a dedicated collection and be frozen at -20 upon recipept at the laboratory. Sample is stable for 3 days at 2-8 degrees. 

For non-rebatable tests the out of pocket cost is $75

Sample must be a dedicated collection and be frozen at -20 upon recipept at the laboratory. Sample is stable for 3 days at 2-8 degrees. 

Laboratory

Referred Test

Biochemistry

Collection & Request Instructions

For non-rebatable tests the out of pocket cost is $60

For non-rebatable tests the out of pocket cost is $60

Sample must be a dedicated collection and be frozen at -20 upon recipept at the laboratory. Sample is stable for 3 days at 2-8 degrees. 

External Laboratory

Monash Pathology

Collection & Request Instructions

For non-rebatable tests the out of pocket cost is $60

Volume (Adults)

20 g

Minimum/Paediatric Volume

1 g

Collection & Request Instructions

Samples can be sent frozen.

External Laboratory

Monash Pathology

Frequency

Weekly

Notes

Contact

Monash University, Eastern Health
Department of Gastroenterology
IBD Gastroenterology Laboratory
Level 1, 5 Arnold St, Box Hill  Vic  3128
Ph: 03 9895 4632
Email: [email protected]

Link to test information and costs :

http://www.med.monash.edu.au/ehcs/services/metabolite/calprotectindrsinformation.html

Request Group

CALPRO

Date

Field

Changed From

Changed To

Alternative names

Dengue fever serology, Kunjin serology, Murray Valley Encephalitis serology

Dengue fever serology, Kunjin serology, Murray Valley Encephalitis serology, Japanese B encephalitis

Request Group

SDENG, OPCR, (SMVE, SJBE)

SDENG, SMVE, SJBE

Preferred Specimen Type

Blood

Blood
Serum

Preferred Container Type

EDTA

EDTA
Serum tube with gel

Request Group

OPCR

SDENG, OPCR, (SMVE, SJBE)

Notes

Procedure detects flaviviruses including Murray Valley encephalitis, dengue and kunjin viruses

PCR testing detects flaviviruses including Murray Valley encephalitis, dengue and kunjin viruses. Dengue specific serology tests for IgG, IgM, and NS1 antigen

Date

Field

Changed From

Changed To

External Laboratory

Sydney South West Pathology Service(RPA)

Dorevitch Pathology

External Laboratory

St Vincents Hospital

Sydney South West Pathology Service(RPA)

Date

Field

Changed From

Changed To

External Laboratory

Monash Pathology

Notes

Arrangements can be made through Microbiology, Monash Medical Centre

Date

Field

Changed From

Changed To

External Laboratory

Victorian Infectious Diseases Reference Laboratory (V.I.D.R.L)

Method

 Contact VIDRL 9342 2649/9342 2650.

Date

Field

Changed From

Changed To

Processing Instructions

Perfomed by Abbott Alinity M

Method

Performed by Abbott Alinity M

Assay name

Hepatitis B Quantitation

Hepatitis B Viral Load

Alternative names

Hepatitis B Viral Load

Hepatitis B Viral Load, Hep B

Laboratory

Referred Test

Microbiology

Request Group

HEPBVL

HBVL

Processing Instructions

Contact VIDRL 9342 9615

Perfomed by Abbott Alinity M

External Laboratory

Victorian Infectious Diseases Reference Laboratory (V.I.D.R.L)

Date

Field

Changed From

Changed To

Volume (Adults)

0.3 mL

0.2 mL

Notes

Test performed is a multiplex PCR for the following viruses: Herpes simplex 1 virus (HSV-1); Herpes simplex 2 virus (HSV-2); Varicella zoster virus (VZV); Cytomegalovirus (CMV); Enterovirus (EV); human Adenovirus (hAdv). Swabs and CSF samples are performed in-house at Box Hill laboratory, other sample types are referred.

Test performed is a multiplex PCR for the following viruses: Herpes simplex 1 virus (HSV-1); Herpes simplex 2 virus (HSV-2); Varicella zoster virus (VZV); Cytomegalovirus (CMV); Enterovirus (EV); human Adenovirus (hAdv). Swabs and CSF samples are performed in-house at Box Hill laboratory, other sample types are referred.

CSF minimum volume for testing: 200uL/0.2mL

Alternative names

CMV PCR, HSV, HSV I-II, HSV PCR, VZV and CMV NAD, VZV PCR

CMV PCR, HSV, HSV I-II, HSV PCR, VZV and CMV NAD, VZV PCR, Enterovirus PCR, Adenovirus PCR

Laboratory

Referred Test

Microbiology

Request Group

HPCR

HPCR, (ENTPCR, ADEPCR)

Preferred Specimen Type

Cerebrospinal Fluid
Fluid
Tissue
Nasopharyngeal swab
Blood

Swab
Cerebrospinal Fluid
Tissue
Nasopharyngeal swab
Respiratory samples

Optional Specimen Type

Respiratory samples

Blood

External Laboratory

Monash Pathology

External Transport Instructions

Tests included in Monash PCR - Herpes simplex 1 virus, Herpes simplex 2 virus, Varicella zoster virus, Cytomegalovirus, Enterovirus (swabs only), Adenovirus (not faeces)

Specimens tested at Monash-

Swabs (dry or in UTM), Sterile fluids, Respiratory samples, Urine.

Specimens anticoagulated with heparin are unsuitable as heparin inhibits PCR

For other specimens - refer to VIDRL. Tissue, CSF, saliva, serum, Plasma (ACD or EDTA),

If  CSF Herpes and Enterovirus PCR -send to VIDRL.

Refer to Referred Molecular Test Requests WORK-MI-90

Specimens tested at Box Hill - Swabs (dry or in UTM), CSF

Specimens from other sites are referred to VIDRL for testing.

Method

Contact Monash Pathology 9594 4532 or if no answer try Bacteriology 9594 4565 as there is someone rostered 24/7

Tested M, W, F

Routine runs are performed Monday and Thursday. Additional runs for urgent CSF samples are perfomed after discussion with the Clinical Microbiologist.

Notes

This test detects Herpes Simplex Virus 1(HSV-1), Herpes Simplex Virus 2 (HSV-2), Varicella zoster virus (VZV) and Cytomegalovirus (CMV).

Test performed is a multiplex PCR for the following viruses: Herpes simplex 1 virus (HSV-1); Herpes simplex 2 virus (HSV-2); Varicella zoster virus (VZV); Cytomegalovirus (CMV); Enterovirus (EV); human Adenovirus (hAdv). Swabs and CSF samples are performed in-house at Box Hill laboratory, other sample types are referred.

Date

Field

Changed From

Changed To

Processing Instructions

During Business Hours: all Fresh Tissue for histopathology must be brought to the attention of an Anatomical Pathologist in the first instance. After Hours: All Fresh Tissue specimens (with the exception of “products of conception” – including placenta)  the on-call Pathologist must be contacted for further instructions. Products of conception including placenta can be stored in the fridge until the next working day.

During Business Hours (0800-1630hours): ALL Fresh Tissue for histopathology must be brought to the attention of an Anatomical Pathologist in the first instance by the receipting/accepting laboratory. After Hours: All Fresh Tissue specimens (with the exception of “products of conception” – including placenta)  the on-call Pathologist must be contacted for further instructions. Products of conception including placenta can be stored in the fridge until the next working day.

Date

Field

Changed From

Changed To

External Laboratory

Austin Pathology

Date

Field

Changed From

Changed To

Alternative names

Kleihauer test

Kleihauer test, FMH (feto-maternal Haemorrhage)

Request Group

KL

FMH

Date

Field

Changed From

Changed To

Frequency

Daily

Mon-Fri

Date

Field

Changed From

Changed To

Alternative names

Thick and Thin films

Malaria parasite investigation Thick and Thin films

Processing Instructions

• Clinical history including details of overseas travel and prophylaxis required
• An FBE is performed at the same time
• ICT test (rapid whole blood immunochromatographic test) for the qualitative detection of Plasmodium falciparum and Plasmodium vivax antigens is performed in addition to microscopic examination.

Clinical history including details of overseas travel and prophylaxis required

An FBE is performed at the same time

ICT test (rapid whole blood immunochromatographic test) for the qualitative detection of Plasmodium falciparum and Plasmodium vivax antigens is performed in addition to microscopic examination.

Notes

Date

Field

Changed From

Changed To

Laboratory

Microbiology

Biochemistry

Request Group

SMEA

MEA

Frequency

Daily

Twice weekly

Date

Field

Changed From

Changed To

Laboratory

Referred Test

Biochemistry

Collection & Request Instructions

Send ASAP (within 24 hrs, 7 days a week). Contact reference laboratory prior to sending the sample.

Testing performed on an as needs basis. 

External Laboratory

Peter MacCallum Cancer Institute

Method

Hand deliover samples to Biochemistry DO NOT load onto the 8100

Date

Field

Changed From

Changed To

Laboratory

Microbiology

Biochemistry

Request Group

SMUM

MUM

Frequency

Daily

Twice weekly

Date

Field

Changed From

Changed To

Collection & Request Instructions

Note: This is an URGENT send out test.

Laboratory hours are 9am - 3pm, Monday - Friday.

Specimen must arrive at Alfred Pathology by 4pm.

Note: This is an URGENT send out test.

Laboratory hours are 9am - 5pm, Monday - Friday.

Specimens need to be delivered to Leve4 Specimen Reception by 3pm as the specimen must arrive at Alfred Pathology by 4pm.

Collection & Request Instructions

Note: This is an URGENT send out test.

Laboratory hours are 9am - 3pm, Monday - Friday.

Note: This is an URGENT send out test.

Laboratory hours are 9am - 3pm, Monday - Friday.

Specimen must arrive at Alfred Pathology by 4pm.

External Transport Instructions

Place the specimen in an esky with ice cubes and dispatch immediately.

Place the specimen in an esky with ice cubes and dispatch immediately. Must arrive at Alfred by 4pm.

Collection & Request Instructions

Note: This is an URGENT send out test.

Laboratory hours are 9am - 5pm, Monday - Friday.

Note: This is an URGENT send out test.

Laboratory hours are 9am - 3pm, Monday - Friday.

Date

Field

Changed From

Changed To

Alternative names

Neonatal Group & DAT Neonatal Group, Screen and DAT

Request Group

BGN

BGN, GPHN

Collection & Request Instructions

Cord blood or heelprick collection required

Cord blood or heelprick collection required

Must be accompanied by a Blood Bank request form with the declaration signed, timed and dated. The blood tube must also be signed, dated and timed; matching the request slip exactly.

Notes

Includes Neonatal ABO & Rh(D) Blood Group and Direct Coombs TestCord blood or heelprick collection required

Includes Neonatal ABO & Rh(D) Blood Group and Direct Coombs

Cord blood or heelprick collection required. 

Date

Field

Changed From

Changed To

Alternative names

ffDNA

Request Group

BBREF, RCAG

External Laboratory

Australian Red Cross Blood Service - Reference Red Cell Laboratory

Laboratory

Unassigned

Referred Test

Collection & Request Instructions

Due to the samples short time limit:

Sample MUST be collected Monday to Wednesday only.

Samples MUST reach BHH Lab by 10am on those days.

Any samples collected outside of these times may be rejected and a recollection asked for.

Processing Instructions

Keep at Room Temperature

External Transport Instructions

Must arrive in QLD within 36 hours on collection

Method

Samples must arrive in QLD within 36 hours of collection.

Samples sent to:  Australian Red Cross Lifeblood QLD

                           44 Musk Ave

                            Kelvin Grove QLD 4059

Samples to be packed as Ambient by an IATA certified person  

Notes

Samples must arrive in QLD within 36 hours of collection.

Samples sent to:  Australian Red Cross Lifeblood QLD

                           44 Musk Ave

                            Kelvin Grove QLD 4059

Date

Field

Changed From

Changed To

Frequency

Daily

Mon-Fri

Notes

Samples must be marked 'URGENT', must reach the laboratory as soon as possible. 

All out-of-hours testing requires notification.

Time and date of collection must be recorded on request form.

Whole blood - 1 x EDTA sample, do not spin. Store at room temperature. An FBE and BLOOD FILM must also be requested,  and needs a separate EDTA sample.

Bone marrow aspirate - Sample into  EDTA tube

Tissue - Fresh (if to be shared with other departments for other tests eg. Histopathology or Microbiology) or RPMI fluid (especially if specimen is taken out of hours). This fluid is obtainable from: BH Lab 9895 3884, MH Lab: 9871 3572 or AH Lab: 9764 6136.

CSF/other fluids

Depending on the tests requested, 3-4 separate tubes will need to be provided. Note that the tubes are numbered so that order of collection can be identified – this order should be carefully followed, so that the optimal sample can be used for the requested tests. It is also important to consider the tests that are required, as this will determine how much CSF is needed in each tube. See the matrix below for details.

TUBE 1 is used for Biochemistry and Flow Cytometry. (Centrifuged in the lab – the supernatant is used for Biochemistry analysis (glucose, protein) and the cellular pellet will be used for flow cytometry if required). If tube 3 is bloodstained, take an aliquot for microbiology.

TUBE 2 is used for Cytology

TUBE 3 is for Microbiological analysis (cell count, Gram stain and culture) and any further infectious testing (e.g. viral PCR, mycobacterial PCR and culture, cryptococcal antigen).

TUBE 4 will be required only for;

(a) Creutzfeld-Jacob Disease (CJD) testing OR

(b) Xanthochromia analysis (wrap the tube in foil to prevent sample deterioration due to light).

Note, the ability to add further tests to a previously collected sample is dependent upon the volume provided. Also, many tests (flow cytometry, cytology) can only be performed on fresh specimens.

Samples must be marked 'URGENT', must reach the laboratory as soon as possible. 

All out-of-hours testing requires notification.

Time and date of collection must be recorded on request form.

Whole blood - 1 x EDTA sample, do not spin. Store at room temperature. An FBE and BLOOD FILM must also be requested,  and needs a separate EDTA sample.

Bone marrow aspirate - Sample into  EDTA tube

Tissue - Fresh (if to be shared with other departments for other tests eg. Histopathology or Microbiology) or RPMI fluid (especially if specimen is taken out of hours). This fluid is obtainable from: BH Lab 9895 3884, MH Lab: 9871 3572 or AH Lab: 9764 6136.

CSF/other fluids

Depending on the tests requested, 3-4 separate tubes will need to be provided. Note that the tubes are numbered so that order of collection can be identified – this order should be carefully followed, so that the optimal sample can be used for the requested tests. It is also important to consider the tests that are required, as this will determine how much CSF is needed in each tube. See the matrix below for details.

TUBE 1 is used for Biochemistry and Flow Cytometry. (Centrifuged in the lab – the supernatant is used for Biochemistry analysis (glucose, protein) and the cellular pellet will be used for flow cytometry if required). If tube 3 is bloodstained, take an aliquot for microbiology.

TUBE 2 is used for Cytology

TUBE 3 is for Microbiological analysis (cell count, Gram stain and culture) and any further infectious testing (e.g. viral PCR, mycobacterial PCR and culture, cryptococcal antigen).

TUBE 4 will be required only for;

(a) Creutzfeld-Jacob Disease (CJD) testing OR

(b) Xanthochromia analysis (wrap the tube in foil to prevent sample deterioration due to light).

Note, the ability to add further tests to a previously collected sample is dependent upon the volume provided. Also, many tests (flow cytometry, cytology) can only be performed on fresh specimens.

Date

Field

Changed From

Changed To

Assay name

Leucocyte Immunophenotyping

Plasma Cell Flow Cytometry Panel

Request Group

LYMP, LEUK

Flow

Volume (Adults)

1 mL

Preferred Container Type

EDTA
Sterile Jar

EDTA
Sterile Jar
CSF

Collection & Request Instructions

Sample store at Room temperature. Samples are not stable for more than 24 hours. Hence they must be received by the Flow Cytometry lab at Box Hill within normal business hours Monday to Friday.

Cut-off for outpatient sample collection on Fridays is at 11.30 AM and MUST reach the laboratory before 4.00PM.

Flow cytometric analysis of cells stained by immunofluorescence with a range of antibodies against lymphoid and myeloid markers.

The panel of markers used will be appropriate to the panel selected and the clinical comments supplied , or as arranged by prior consultation

Sample store at Room temperature. Samples are not stable for more than 24 hours. Hence they must be received by the Flow Cytometry lab at Box Hill within normal business hours Monday to Friday.

Cut-off for outpatient sample collection on Fridays is at 11.30 AM and MUST reach the laboratory before 2.00PM.

Flow cytometric analysis of cells stained by immunofluorescence with a range of antibodies against lymphoid and myeloid markers.

The panel of markers used will be appropriate to the panel selected and the clinical comments supplied , or as arranged by prior consultation

Frequency

Daily

Notes

Samples must be marked 'URGENT', must reach the laboratory as soon as possible. 

All out-of-hours testing requires notification.

Time and date of collection must be recorded on request form.

Whole blood - 1 x EDTA sample, do not spin. Store at room temperature. An FBE and BLOOD FILM must also be requested,  and needs a separate EDTA sample.

Bone marrow aspirate - Sample into  EDTA tube

Tissue - Fresh (if to be shared with other departments for other tests eg. Histopathology or Microbiology) or RPMI fluid (especially if specimen is taken out of hours). This fluid is obtainable from: BH Lab 9895 3884, MH Lab: 9871 3572 or AH Lab: 9764 6136.

CSF/other fluids

Depending on the tests requested, 3-4 separate tubes will need to be provided. Note that the tubes are numbered so that order of collection can be identified – this order should be carefully followed, so that the optimal sample can be used for the requested tests. It is also important to consider the tests that are required, as this will determine how much CSF is needed in each tube. See the matrix below for details.

TUBE 1 is used for Biochemistry and Flow Cytometry. (Centrifuged in the lab – the supernatant is used for Biochemistry analysis (glucose, protein) and the cellular pellet will be used for flow cytometry if required). If tube 3 is bloodstained, take an aliquot for microbiology.

TUBE 2 is used for Cytology

TUBE 3 is for Microbiological analysis (cell count, Gram stain and culture) and any further infectious testing (e.g. viral PCR, mycobacterial PCR and culture, cryptococcal antigen).

TUBE 4 will be required only for;

(a) Creutzfeld-Jacob Disease (CJD) testing OR

(b) Xanthochromia analysis (wrap the tube in foil to prevent sample deterioration due to light).

Note, the ability to add further tests to a previously collected sample is dependent upon the volume provided. Also, many tests (flow cytometry, cytology) can only be performed on fresh specimens.

Other specialised testing (e.g. oligoclonal bands) will be performed using the supplied samples.

Xanthochromia and CJD analysis cannot be performed on a blood-stained sample

Samples must be marked 'URGENT', must reach the laboratory as soon as possible. 

All out-of-hours testing requires notification.

Time and date of collection must be recorded on request form.

Whole blood - 1 x EDTA sample, do not spin. Store at room temperature. An FBE and BLOOD FILM must also be requested,  and needs a separate EDTA sample.

Bone marrow aspirate - Sample into  EDTA tube

Tissue - Fresh (if to be shared with other departments for other tests eg. Histopathology or Microbiology) or RPMI fluid (especially if specimen is taken out of hours). This fluid is obtainable from: BH Lab 9895 3884, MH Lab: 9871 3572 or AH Lab: 9764 6136.

CSF/other fluids

Depending on the tests requested, 3-4 separate tubes will need to be provided. Note that the tubes are numbered so that order of collection can be identified – this order should be carefully followed, so that the optimal sample can be used for the requested tests. It is also important to consider the tests that are required, as this will determine how much CSF is needed in each tube. See the matrix below for details.

TUBE 1 is used for Biochemistry and Flow Cytometry. (Centrifuged in the lab – the supernatant is used for Biochemistry analysis (glucose, protein) and the cellular pellet will be used for flow cytometry if required). If tube 3 is bloodstained, take an aliquot for microbiology.

TUBE 2 is used for Cytology

TUBE 3 is for Microbiological analysis (cell count, Gram stain and culture) and any further infectious testing (e.g. viral PCR, mycobacterial PCR and culture, cryptococcal antigen).

TUBE 4 will be required only for;

(a) Creutzfeld-Jacob Disease (CJD) testing OR

(b) Xanthochromia analysis (wrap the tube in foil to prevent sample deterioration due to light).

Note, the ability to add further tests to a previously collected sample is dependent upon the volume provided. Also, many tests (flow cytometry, cytology) can only be performed on fresh specimens.

Other specialised testing (e.g. oligoclonal bands) will be performed using the supplied samples.

Xanthochromia and CJD analysis cannot be performed on a blood-stained sample

Date

Field

Changed From

Changed To

Assay name

Plasma Free Heamoglobin

Plasma Free Haemoglobin

Collection & Request Instructions

Samples must be hand delivered to the lab to avoid shaking, which will falsely raise the plasma free-haemoglobin. Normal operating plasma free-haemoglobin level is < 0.10 g/dL. This is performed 24-hourly whilst on ECMO (routinely taken with the daily blood samples) according to clinical need/ECMO clinician direction.

Samples are taken by passively filling a 10mL syringe carefully from the intra-arterial line access port. Do not connect to a negative pressure “vacutainer” for sampling. The plasma free-haemoglobin collection tube is filled from the syringe after removing the lid, gently injecting blood from the syringe and then replacing the lid. No needles are required.

Preferred Specimen Type

Plasma

Serum

Preferred Container Type

Serum tube with gel

Lithium heparin no gel

Date

Field

Changed From

Changed To

Assay name

PML:RARA, RUNX1,CBFB:MYH11 Screening

PML:RARA, RUNX1,CBFB:MYH11 MRD/Screening

Collection & Request Instructions

Samples to be kept at room temperature.

Do not spin.

Do not freeze.

Paediatric EDTA tubes cannot be used for these tests.

Samples to be kept at room temperature.

Do not spin.

Do not freeze.

Date

Field

Changed From

Changed To

Method

All PCT requests must be discussed with the Chemical Pathologist upon receipt in lab, regardless of time of day or day of week. If urgent/Taxi requiring is determined, result must be phoned to the clinician upon receipt - High Risk Result.

Method

All PCT requests must be discussed with the Chemical Pathologist upon receipt in lab, regardless of time of day or day of week. If urgent/Taxi requiring is determined, result must be phoned to the clinician upon receipt - High Risk Result.

Date

Field

Changed From

Changed To

Laboratory

Referred Test

Biochemistry

External Laboratory

Peter MacCallum Cancer Institute

Notes

Routinely, a qualitative, interpretive report of a capillary electrophoresis is made. It is important to provide adequate clinical information with the request for these interpretations to be meaningful.

comprehensive clincial notes are essential. 

As of 14/06/2024 Serum Protein Electrophoresis performed by Sebia gel electrophoresis at Eastern Health. 

Date

Field

Changed From

Changed To

External Laboratory

ICPMR (Institute of Clinical Pathology and Medical Research)

Method

Contact VIDRL 9342 2649 / 9342 2650

Test performed by Institute of Clinical Pathology and Medical Research (ICPMR), NSW

External Laboratory

Victorian Infectious Diseases Reference Laboratory (V.I.D.R.L)

Date

Field

Changed From

Changed To

Alternative names

QTFN, Quantiferon TB Gold

Quantiferon, TB Gold, QTFN

Frequency

Twice weekly

Weekly

Date

Field

Changed From

Changed To

Collection & Request Instructions

White top tube is the preferred sample.0.2ml of blood required for each allergen.

Blood collection: still can be done after skin allergy testing.

Omega-5-Gliadin, Alpha Gal and Cefaclor : serum send to Melbourne Pathology

IgG 96 Food Allergy test is not a RAST test  It is performed at  Australian Clinical Labs.This test is NOT rebatable and costs approx  $260.

White top tube is the preferred sample.0.2ml of blood required for each allergen.

Blood collection: still can be done after skin allergy testing.

RAST for Omega-5-Gliadin to be sent to Alfred Pathology, Alpha Gal to be sent to Melbourne Pathology.                               Cefaclor and ARAh2 to be sent to Dorevitch Pathology 

IgG 96 Food Allergy test is not a RAST test  It is performed at  Australian Clinical Labs.This test is NOT rebatable and costs approx  $260.

Processing Instructions

Omega-5-Gliadin: send to Melborne Pathology

IgG 96 Food Allergy test is not a RAST test  It is performed at  Australian Clinical Labs.This test is NOT rebatable and costs approx  $260.

IgG 96 Food Allergy test is not a RAST test  It is performed at  Australian Clinical Labs.This test is NOT rebatable and costs approx  $260.

Method

RAST for Omega-5-Gliadin to be sent to Alfred Pathology, Alpha Gal to be sent to Melbourne Pathology.                               Cefaclor and ARAh2 to be sent to Dorevitch Pathology 

Date

Field

Changed From

Changed To

Collection & Request Instructions

Collection Requirements for both or either tests

Collect into 4 mL EDTA tubes at Room Temperature. Do not collect on ice

If both Renin and Aldosterone are requested together, they can be collected into the same EDTA tube

In the laboratory, spin and separate the plasma immediately. Plasma is stable at Room Temperature if analysis is performed on the same day.

Renin must be frozen at -80 C. Transport plasma at room temperature (Maroondah and Angliss) until it can be placed in -80 C Freezer.(Must be done within 3-4 hors).

Add-on tests for Renin and Aldosterone should not be done on samples which have been previously collected or stored at 4 C.

Renin will not be collected at Healesville Hospital and Yarra Ranges Health Collection Rooms.

Collection Requirements for both or either tests

Collect into 4 mL EDTA tubes at Room Temperature. Do not collect on ice

If both Renin and Aldosterone are requested together, they can be collected into the same EDTA tube

In the laboratory, spin and separate the plasma immediately. Plasma is stable at Room Temperature if analysis is performed on the same day.

Renin must be frozen at -80 C. Transport plasma at room temperature (Maroondah and Angliss) until it can be placed in -80 C Freezer.(Must be done within 3-4 hours).

Add-on tests for Renin and Aldosterone should not be done on samples which have been previously collected or stored at 4 C.

Renin will not be collected at Healesville Hospital and Yarra Ranges Health Collection Rooms.

Samples from Angliss and Maroondah must be collected within business hours to allow transport to Box Hill via courier.

Laboratory

Referred Test

Biochemistry

External Laboratory

Austin Pathology

Date

Field

Changed From

Changed To

Method

The following specimens will be referred for testing-

Sputum, Bronchial Washings, Bronchoalveolar Lavages - VIDRL

Swab specimens for Respiratory Multiplex PCR will be referred to Monash Molecular Laboratory

The following specimens will be referred for testing-

Sputum, Bronchial Washings, Bronchoalveolar Lavages - VIDRL

As of Feb 2023 swab specimens for Respiratory Multiplex PCR will be tested in-house.

Preferred Specimen Type

Nasopharyngeal swab
Nasopharyngeal Aspirate

Nasopharyngeal swab
NTS - nose & throat swab
Nasopharyngeal Aspirate

Optional Specimen Type

Sputum
Bronchial Washings
Broncho-alveolar Lavage

Preferred Container Type

Viral Transport Media- Nasopharyngeal
Respiratory aspiration pot

Viral Transport Media- Nasopharyngeal
Respiratory aspiration pot
respiratory PCR requirements

Collection & Request Instructions

Please note these swabs are available from stores.

Search for: VIRAL TRANSPORT MEDIA - NASOPHARYNGEAL

Pathology do not keep a stock of these swabs. Emergency departments and wards should manage a supply of these swabs according to demand.

Please note these swabs are available from stores.

Pathology do not keep a stock of these swabs. Emergency departments and wards should manage a supply of these swabs according to demand.

Method

All tests for respiratory virus PCR are considered urgent and must be delivered to the laboratory ASAP.

ICU/Oncology patients testing negative for Flu A/B and RSV  will be referred to Monash for respiratory virus panel. This includes Influenza A virus, Influenza B virus, Respiratory Syncitial Virus, Picornavirus (RV/EV), Parainfluenza viruses 1,2 and 3, Adenovirus, Human Metapneumovirus, Bordetella pertussis, Bordetella parapertussis, Mycoplasma pneumoniae, Legionella species, Chlamydiaceae.

The following specimens will be referred for testing-

Sputum, Bronchial Washings, Bronchoalveolar Lavages

Specimens for Respiratory Multiplex PCR will be referred to Monash Molecular Laboratory

Contact Monash Pathology 9594 4532 or if no answer try Bacteriology 9594 4565 as there is someone rostered 24/7

The following specimens will be referred for testing-

Sputum, Bronchial Washings, Bronchoalveolar Lavages - VIDRL

Swab specimens for Respiratory Multiplex PCR will be referred to Monash Molecular Laboratory

Notes

This PCR will detect and identify: Influenza A virus, Influenza B virus and Respiratory syncytial virus (RSV).

Transport at room temperature to the Laboratory

Testing is performed at Box Hill, Angliss, and Maroondah.

Samples received after hours may have a longer TAT due to other urgent critical pathology testing occurring.

This PCR will detect and identify: Influenza A virus, Influenza B virus and Respiratory syncytial virus (RSV).

Transport at room temperature to the Laboratory

Testing is performed at Box Hill, Angliss, and Maroondah.

DO NOT send samples in the Pathology Chute

For Rapid testing from Emergency Department - swab must be accompanied by signed Rapid Test Authorisation Form.

DO NOT call the laboratory for results/enquiries. Inpatient tests will be prioritised and results available on EMR ASAP.

Sputum samples are referred to an external laboratory - results will be delayed.

Date

Field

Changed From

Changed To

Preferred Container Type

Viral Transport Media - Nasopharyngeal

Viral Transport Media - Nasopharyngeal
respiratory PCR requirements
Viral Transport Media- Nasopharyngeal

Preferred Specimen Type

NTS - nose & throat swab
Swab

NTS - nose & throat swab
Respiratory samples

Date

Field

Changed From

Changed To

Frequency

Weekly

As required

Method

Test performed by Institute of Clinical Pathology and Medical Research (ICPMR), NSW

Notes

Tests for Total Ab, IgM  response to Spotted fever, Typhus and Scrub Typhus groups.

Tests for serology response to Spotted fever, Typhus and Scrub Typhus groups.

External Laboratory

Victorian Infectious Diseases Reference Laboratory (V.I.D.R.L)

Date

Field

Changed From

Changed To

Frequency

Weekly

Fortnightly

Date

Field

Changed From

Changed To

Collection & Request Instructions

Soluscope should be sent to laboratory in sterile container

Preferred Specimen Type

Culture

not required

Preferred Specimen Type

not required

Culture

Preferred Container Type

Not required

Sterile Jar

Frequency

As required

Date

Field

Changed From

Changed To

Processing Instructions

Samples are be aliquoted and frozen in the barcoded tube supplied in the JCV kit

Notes

Samples are be aliquoted and frozen in the barcoded tube supplied in the JCV kit

Date

Field

Changed From

Changed To

Preferred Container Type

Lithium heparin no gel
EDTA

See Instructions

Collection & Request Instructions

Collect 1 x 4 ml Lithium Heparin tube and

1 x 4ml EDTA tube.

FBE must be requested with this test as a RCC is required for calculation of results

DO NOT SPIN.

Keep at Room Temp.

Collect 1 x 4 ml Lithium Heparin tube and

2 x 4ml EDTA tube.

FBE must be requested with this test as a RCC is required for calculation of results

DO NOT SPIN.

Keep at Room Temp.

Collection & Request Instructions

Collect 1 x 4 ml Lithium Heparin tube and 1 x 4ml EDTA tube.

FBE must be requested with this test as a RCC is required for calculation of results

DO NOT SPIN.

Keep at Room Temp.

Collect 1 x 4 ml Lithium Heparin tube and

1 x 4ml EDTA tube.

FBE must be requested with this test as a RCC is required for calculation of results

DO NOT SPIN.

Keep at Room Temp.

Request Group

TMETAB

Preferred Container Type

EDTA

Lithium heparin no gel
EDTA

Collection & Request Instructions

Transport specimen at Room Temperature to lab

Collection & Request Instructions

Collect 2x4ml EDTA blood. DO NOT SPIN

Keep at 4 deg C, do not freeze.

The metabolites are stable for upto 4 days at 4 degress.

Collect 1 x 4 ml Lithium Heparin tube and 1 x 4ml EDTA tube.

FBE must be requested with this test as a RCC is required for calculation of results

DO NOT SPIN.

Keep at Room Temp.

Processing Instructions

Keep samples at Room Temperature.

Do Not Spin. Whole Blood required

External Laboratory

St Vincents Hospital

Frequency

Weekly

Notes

Imuran is a trade name for azathioprine

The Thiopurine metabolites (6-TGN & 6-MMP) are used as a means of estimating the toxicity and efficacy of azathioprine treatment.

This test is performed by the Gastroenerology department. ( Phone No.9895 4632 )

The cost for the test is not covered by Medicare. 

Eastern Health patients:6TGN 6MMP Thiopurine Metabolites Testing $75

All other patients: 6TGN 6MMP Thiopurine Metabolites Testing $145

Imuran is a trade name for azathioprine

The Thiopurine metabolites (6-TGN & 6-MMP) are used as a means of estimating the toxicity and efficacy of azathioprine treatment.

Date

Field

Changed From

Changed To

Request Group

STOX

TOX

Date

Field

Changed From

Changed To

Processing Instructions

The serum must be aliquoted and frozen. Transthyretin is only stable for 3 days at 4-8C .

The serum must be aliquoted and frozen. Transthyretin is only stable for 3 days at 4-8C .

External Laboratory

Monash Pathology

Date

Field

Changed From

Changed To

Alternative names

enteric fever, Typhoid fever

enteric fever, typhoid fever

Preferred Container Type

Blood Culture

Blood Culture (Adult)
Blood Culture (Paediatric)

Date

Field

Changed From

Changed To

External Laboratory

Australian Clinical Labs (formerly Healthscope)

St Vincents Hospital

Date

Field

Changed From

Changed To

External Laboratory

Australian Clinical Labs (formerly Healthscope)

St Vincents Hospital

Date

Field

Changed From

Changed To

Laboratory

Unassigned

Haematology

Frequency

Weekly

Fortnightly

Date

Field

Changed From

Changed To

Laboratory

Referred Test

Haematology

External Laboratory

Austin Pathology

Frequency

Weekly

Fortnightly