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Recent Changes - Test & Collection Guide

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 Recent changes for Adrenocorticotrophic Hormone

Recent changes for Adrenocorticotrophic Hormone

Date
Field
Changed From
Changed To
2nd August 2022
Notes

Consultation with Endocrinologist suggested

Collect in the morning between 0800 and 1000h.

1 x 4mL EDTA tube on ice.

Send to laboratory for immediate centrifugation. Spin, separate and freeze plasma. Transport frozen.

 

 

 

Consultation with Endocrinologist suggested

Collect 1 x 4mL EDTA tube on ice.

Send to laboratory for immediate centrifugation. Spin, separate and freeze plasma. 

 

 

 


 Recent changes for Aldosterone - blood

Recent changes for Aldosterone - blood

Date
Field
Changed From
Changed To
23rd May 2023
Laboratory

Referred Test

Biochemistry

Collection & Request Instructions

 

Collection Requirements for both or either tests

Collect into 4 mL EDTA tubes at Room Temperature. Do not collect on ice

If both Renin and Aldosterone are requested together, they can be collected into the same EDTA tube

In the laboratory, spin and separate the plasma immediately. Plasma is stable at Room Temperature if analysis is performed on the same day

If the specimen arrives on the weekend or after hours, or if the specimen is referred from an external laboratory, freeze the plasma

EDTA plasma samples from external laboratories should be stored and transported frozen

Whole blood or plasma stored at 4 Deg C is not suitable

Add-on tests for Renin and Aldosterone should not be done on samples which have been previously collected or stored at 4 Deg C

 

Collection Requirements for both or either tests

Collect into 4 mL EDTA tubes at Room Temperature. Do not collect on ice

If both Renin and Aldosterone are requested together, they can be collected into the same EDTA tube

In the laboratory, spin and separate the plasma immediately. Plasma is stable at Room Temperature if analysis is performed on the same day

If the specimen arrives on the weekend or after hours freeze the plasma

EDTA plasma samples from external laboratories should be stored and transported frozen

Whole blood or plasma stored at 4 Deg C is not suitable

Add-on tests for Renin and Aldosterone should not be done on samples which have been previously collected or stored at 4 Deg C

External Laboratory

Austin Pathology


 Recent changes for Alpha-1 Antitrypsin - Quantitation

Recent changes for Alpha-1 Antitrypsin - Quantitation

Date
Field
Changed From
Changed To
13th September 2022
External Laboratory

St Vincents Hospital


 Recent changes for Anti-Heart Antibodies

Recent changes for Anti-Heart Antibodies

Date
Field
Changed From
Changed To
28th September 2023
External Laboratory

Pathwest Lab Med WA


 Recent changes for Anti-Myocardial Antibodies

Recent changes for Anti-Myocardial Antibodies

Date
Field
Changed From
Changed To
28th September 2023
External Laboratory

Pathwest Lab Med WA


 Recent changes for Aspergillus precipitins

Recent changes for Aspergillus precipitins

Date
Field
Changed From
Changed To
23rd November 2023
Frequency

Twice weekly

Weekly

23rd November 2023
Collection & Request Instructions

Immunodiffusion for Aspergillus fumigatus antibodies in allergic Aspergillosis.

Test for Aspergillus fumigatus antibodies.

23rd November 2023
External Laboratory

Monash Pathology

Method

Test performed by Royal North Shore Hospital, NSW

Sent with ICPMR courier


 Recent changes for Azathioprine Metabolite Testing

Recent changes for Azathioprine Metabolite Testing

Date
Field
Changed From
Changed To
23rd August 2022
Processing Instructions

Place the samples and request slip in fridge (M43) in CSR. Do not enter in the computer.

Eastern Health Clinical School
Level 2, 5 Arnold Street,
Box Hill  Vic  3128
Ph: 61 3 9091 8827
Fax: 61 3 9899 9137
E-mail:  ehcs@monash.edu


 Recent changes for Bile Acids

Recent changes for Bile Acids

Date
Field
Changed From
Changed To
23rd May 2022
Laboratory

Referred Test

Biochemistry

Preferred Container Type

Plain Tube with gel

Serum tube with gel

Collection & Request Instructions

Fasting or Non Fasting sample.

Note: This test is ONLY performed on pregnant women.

 

Fasting.

Note: This test is ONLY performed on pregnant women.

 

Processing Instructions

Spin, separate ASAP.

External Laboratory

Royal Childrens Hospital

Frequency

Weekly

Twice weekly

Method

Store and transport at 4 C.


 Recent changes for Bone Marrow Examination

Recent changes for Bone Marrow Examination

Date
Field
Changed From
Changed To
25th March 2022
Laboratory

Unassigned

Haematology


 Recent changes for Cadmium

Recent changes for Cadmium

Date
Field
Changed From
Changed To
13th September 2022
Notes

Transport specimen at Room Temperature to lab
Sample haemolysis may make this test unreportable

 

 

Transport specimen at Room Temperature to lab
Sample haemolysis may make this test unreportable

Send as whole blood

 

 


 Recent changes for Caeruloplasmin

Recent changes for Caeruloplasmin

Date
Field
Changed From
Changed To
13th September 2022
External Laboratory

Austin Pathology


 Recent changes for Cholesterol, Low Density Lipoprotein

Recent changes for Cholesterol, Low Density Lipoprotein

Date
Field
Changed From
Changed To
29th February 2024
Notes

 

LDL is not measured directly, but is calculated using the Friedwald formula:

(LDLC) = (total CHOL) - (HDLC) - (TG/2.2)

This formula cannot be used where TG > 4.5 mmol/L

LDL was previously calculated from triglyceride, cholesterol and HDL cholesterol using the Friedewald formula.


As from 22nd of February 2024, LDL is calculated using the Sampson equation.
Friedewald knew that with increasing triglyceride levels, the calculated LDL-C was progressively falsely lower when compared with the gold standard technique and therefore Friedewald stated LDL-C should not be calculated when triglycerides are > 4.5 mmol/L. With much lower LDL-C levels seen in the statin era, an improved calculation was required, hence the Sampson equation.


Comparing both equations with the gold standard ultracentrifugation method, Sampson LDL-C offers similar or better accuracy than Friedewald LDL-C at any triglyceride level. Sampson LDL-C can also be calculated up to a triglyceride level of 9 mmol/L.


Friedewald: LDL-C = TC – HDL-C – Triglycerides/2.2

Sampson: LDL-C=TC/0.948−HDL-C/0.971−(TG/8.56+TG × non-HDL-C/2140 −TG2/16100)−9.44


The calculation for the Sampson equation is complicated but Friedewald LDL-C can be (easily) calculated from the available lipid results if required.
 

 


 Recent changes for CMV Serology

Recent changes for CMV Serology

Date
Field
Changed From
Changed To
18th January 2023
Notes

IgG and IgM, IgG Avidity Index performed

 

IgG and IgM, IgG Avidity Index performed if IgM is positive

 

18th January 2023
Laboratory

Microbiology

Biochemistry


 Recent changes for Creutzfeldt-Jakob Disease

Recent changes for Creutzfeldt-Jakob Disease

Date
Field
Changed From
Changed To
1st September 2023
Collection & Request Instructions

Requests for Alzheimer's disease - CSF must be collected in a seperate blue capped PP tube, available from Pathology Specimen Reception (CSR).

Processing Instructions

DO NOT CENTRIFUGE SPECIMEN

This test requires authorisation by the Neurology Registrar or Neurology Consultant.

CSF Specimens are unsuitable for testing if macroscopically haemorrhagic, xanthochromic, have red blood cell counts < 500/uL, have white cell counts < 10/uL

DO NOT CENTRIFUGE SPECIMEN

This test requires authorisation by the Neurology Registrar or Neurology Consultant.

CSF Specimens are unsuitable for testing if macroscopically haemorrhagic, xanthochromic. Cell count must have red blood cell counts < 500/uL, and white cell counts < 10/uL

Notes

This test requires authorisation by the Neurology Registrar or Neurology Consultant.

In addition, please refer to Eastern Health Infection Control CJD procedure and contact Infection Control prior to performing the Lumbar Puncture

Test is run weekly. Samples received by 4 p.m. Friday will have the result issued by the end of the following week.

 

CSF : Tests for 14-3-3 CSF protein

At least 1 mL of CSF is required in a separate (4th), unopened tube.

Double bag specimen. Must be frozen and transported on dry ice.

Blood : Test for PRNP (Prion protein gene)

5 mLs in EDTA tube

This test requires authorisation by the Neurology Registrar or Neurology Consultant.

In addition, please refer to Eastern Health Infection Control CJD procedure and contact Infection Control prior to performing the Lumbar Puncture

Test is run weekly. Samples received by 4 p.m. Friday will have the result issued by the end of the following week.

 

CSF : Tests for 14-3-3 CSF protein

At least 1 mL of CSF is required in a separate (4th), unopened tube.

Double bag specimen. Note: sample is no longer required to be sent frozen.

Blood : Test for PRNP (Prion protein gene)

5 mLs in EDTA tube


 Recent changes for CSF BioFire Meningitis/Encephalitis Panel

Recent changes for CSF BioFire Meningitis/Encephalitis Panel

Date
Field
Changed From
Changed To
11th September 2023
Collection & Request Instructions

This test required approval from the Clinical Microbiologist. Minimum 200uL/0.2mL of unspun CSF is required for this test.

This test required approval from the Clinical Microbiologist. Minimum 200uL/0.2mL of unspun CSF is required for this test.

CSF collected via lumbar punture only; bloodstained/xanthochromic samples are suitable

Notes

NOTE: Non-K1 E. coli serotypes may be present in a specimen and will not be detected by the BioFire ME Panel.
NOTE: Non-encapsulated strains of Neisseria meningitidis are not detected by the BioFire ME Panel.
NOTE: The BioFire ME Panel does not distinguish between latent and active CMV and HHV-6 infections. Detection of these viruses may indicate primary infection, secondary reactivation, or the presence of latent virus. Results should always be interpreted in conjunction with other clinical, laboratory, and epidemiological information.
NOTE: Patients with a suspicion of cryptococcal meningitis and a negative cryptococcal PCR result, such as by the BioFire ME Panel, should be tested for CrAG.


 Recent changes for EBV Serology

Recent changes for EBV Serology

Date
Field
Changed From
Changed To
18th January 2023
Laboratory

Microbiology

Biochemistry


 Recent changes for Erythrocyte Sedimentation Rate

Recent changes for Erythrocyte Sedimentation Rate

Date
Field
Changed From
Changed To
10th May 2023
Volume (Adults)

1.5 mL

1 mL

Minimum/Paediatric Volume

0.5 mL

Preferred Container Type

ESR tube

EDTA
EDTA - Paediatric

Collection & Request Instructions

Using the ESR tube :
Transport specimen at Room Temperature to lab
Sample haemolysis may make this test unreportable

Transport specimen at Room Temperature to lab
Sample haemolysis may make this test unreportable
Samples found to have clots cannot be tested

Collection & Request Instructions

This tube consists of an inner vessel with an outer sleeve.  The outer sleeve MUST remain in place as this is where sample identification (label or handwritten) will be placed. 

Frequency

Daily

Notes

If the ESR test is requested in conjunction with a FBE test request, a single EDTA tube may be shared. In these instances;

Minimum Adult Volume: 1.5 mL

Minimum Paediatric Volume: 1 mL


 Recent changes for Faecal Calprotectin

Recent changes for Faecal Calprotectin

Date
Field
Changed From
Changed To
1st December 2023
Collection & Request Instructions

For non-rebatable tests the out of pocket cost is $60

24th August 2022
Volume (Adults)

20 g

Minimum/Paediatric Volume

1 g

Collection & Request Instructions

Samples can be sent frozen.

External Laboratory

Monash Pathology

Frequency

Weekly

Notes

 

Contact

Monash University, Eastern Health
Department of Gastroenterology
IBD Gastroenterology Laboratory
Level 1, 5 Arnold St, Box Hill  Vic  3128
Ph: 03 9895 4632
Email: ehcs.ibd@monash.edu

Link to test information and costs :

http://www.med.monash.edu.au/ehcs/services/metabolite/calprotectindrsinformation.html

 

 

24th August 2022
Request Group

CALPRO


 Recent changes for Flavivirus Serology

Recent changes for Flavivirus Serology

Date
Field
Changed From
Changed To
1st February 2023
Alternative names

Dengue fever serology, Kunjin serology, Murray Valley Encephalitis serology

Dengue fever serology, Kunjin serology, Murray Valley Encephalitis serology, Japanese B encephalitis

Request Group

SDENG, OPCR, (SMVE, SJBE)

SDENG, SMVE, SJBE

31st January 2023
Preferred Specimen Type

Blood

Blood
Serum

Preferred Container Type

EDTA

EDTA
Serum tube with gel

31st January 2023
Request Group

OPCR

SDENG, OPCR, (SMVE, SJBE)

Notes

Procedure detects flaviviruses including Murray Valley encephalitis, dengue and kunjin viruses

PCR testing detects flaviviruses including Murray Valley encephalitis, dengue and kunjin viruses. Dengue specific serology tests for IgG, IgM, and NS1 antigen


 Recent changes for Helicobacter pylori Breath Test

Recent changes for Helicobacter pylori Breath Test

Date
Field
Changed From
Changed To
3rd January 2024
External Laboratory

Monash Pathology

3rd January 2024
Notes

Arrangements can be made through Microbiology, Monash Medical Centre


 Recent changes for Helicobacter pylori Serology

Recent changes for Helicobacter pylori Serology

Date
Field
Changed From
Changed To
27th October 2023
External Laboratory

Victorian Infectious Diseases Reference Laboratory (V.I.D.R.L)

Method

 Contact VIDRL 9342 2649/9342 2650.


 Recent changes for Hepatitis B Viral Load

Recent changes for Hepatitis B Viral Load

Date
Field
Changed From
Changed To
2nd February 2023
Processing Instructions

Perfomed by Abbott Alinity M

Method

Performed by Abbott Alinity M

2nd February 2023
Assay name

Hepatitis B Quantitation

Hepatitis B Viral Load

Alternative names

Hepatitis B Viral Load

Hepatitis B Viral Load, Hep B

Laboratory

Referred Test

Microbiology

Request Group

HEPBVL

HBVL

Processing Instructions

Contact VIDRL 9342 9615

Perfomed by Abbott Alinity M

External Laboratory

Victorian Infectious Diseases Reference Laboratory (V.I.D.R.L)


 Recent changes for Herpes Virus PCR

Recent changes for Herpes Virus PCR

Date
Field
Changed From
Changed To
7th September 2023
Volume (Adults)

0.3 mL

0.2 mL

Notes

Test performed is a multiplex PCR for the following viruses: Herpes simplex 1 virus (HSV-1); Herpes simplex 2 virus (HSV-2); Varicella zoster virus (VZV); Cytomegalovirus (CMV); Enterovirus (EV); human Adenovirus (hAdv). Swabs and CSF samples are performed in-house at Box Hill laboratory, other sample types are referred.

 

Test performed is a multiplex PCR for the following viruses: Herpes simplex 1 virus (HSV-1); Herpes simplex 2 virus (HSV-2); Varicella zoster virus (VZV); Cytomegalovirus (CMV); Enterovirus (EV); human Adenovirus (hAdv). Swabs and CSF samples are performed in-house at Box Hill laboratory, other sample types are referred.

CSF minimum volume for testing: 200uL/0.2mL

7th September 2023
Alternative names

CMV PCR, HSV, HSV I-II, HSV PCR, VZV and CMV NAD, VZV PCR

CMV PCR, HSV, HSV I-II, HSV PCR, VZV and CMV NAD, VZV PCR, Enterovirus PCR, Adenovirus PCR

Laboratory

Referred Test

Microbiology

Request Group

HPCR

HPCR, (ENTPCR, ADEPCR)

Preferred Specimen Type

Cerebrospinal Fluid
Fluid
Tissue
Nasopharyngeal swab
Blood

Swab
Cerebrospinal Fluid
Tissue
Nasopharyngeal swab
Respiratory samples

Optional Specimen Type

Respiratory samples

Blood

External Laboratory

Monash Pathology

External Transport Instructions

Tests included in Monash PCR - Herpes simplex 1 virus, Herpes simplex 2 virus, Varicella zoster virus, Cytomegalovirus, Enterovirus (swabs only), Adenovirus (not faeces)

Specimens tested at Monash-

Swabs (dry or in UTM), Sterile fluids, Respiratory samples, Urine.

Specimens anticoagulated with heparin are unsuitable as heparin inhibits PCR

For other specimens - refer to VIDRL. Tissue, CSF, saliva, serum, Plasma (ACD or EDTA),

If  CSF Herpes and Enterovirus PCR -send to VIDRL.

Refer to Referred Molecular Test Requests WORK-MI-90

Specimens tested at Box Hill - Swabs (dry or in UTM), CSF

Specimens from other sites are referred to VIDRL for testing.

 

 

 

Method

Contact Monash Pathology 9594 4532 or if no answer try Bacteriology 9594 4565 as there is someone rostered 24/7

Tested M, W, F

Routine runs are performed Monday and Thursday. Additional runs for urgent CSF samples are perfomed after discussion with the Clinical Microbiologist.

Notes

This test detects Herpes Simplex Virus 1(HSV-1), Herpes Simplex Virus 2 (HSV-2), Varicella zoster virus (VZV) and Cytomegalovirus (CMV).

 

Test performed is a multiplex PCR for the following viruses: Herpes simplex 1 virus (HSV-1); Herpes simplex 2 virus (HSV-2); Varicella zoster virus (VZV); Cytomegalovirus (CMV); Enterovirus (EV); human Adenovirus (hAdv). Swabs and CSF samples are performed in-house at Box Hill laboratory, other sample types are referred.

 


 Recent changes for Histopathology

Recent changes for Histopathology

Date
Field
Changed From
Changed To
20th October 2023
Processing Instructions

During Business Hours: all Fresh Tissue for histopathology must be brought to the attention of an Anatomical Pathologist in the first instance. After Hours: All Fresh Tissue specimens (with the exception of “products of conception” – including placenta)  the on-call Pathologist must be contacted for further instructions. Products of conception including placenta can be stored in the fridge until the next working day.

During Business Hours (0800-1630hours): ALL Fresh Tissue for histopathology must be brought to the attention of an Anatomical Pathologist in the first instance by the receipting/accepting laboratory. After Hours: All Fresh Tissue specimens (with the exception of “products of conception” – including placenta)  the on-call Pathologist must be contacted for further instructions. Products of conception including placenta can be stored in the fridge until the next working day.


 Recent changes for IgG Subclasses

Recent changes for IgG Subclasses

Date
Field
Changed From
Changed To
13th September 2022
External Laboratory

Austin Pathology


 Recent changes for Infectious Mononucleosis Screen

Recent changes for Infectious Mononucleosis Screen

Date
Field
Changed From
Changed To
30th March 2022
Preferred Specimen Type

Serum

Serum
Blood

Optional Specimen Type

Plasma

Preferred Container Type

Serum tube with gel

Serum tube with gel
EDTA
EDTA - Paediatric


 Recent changes for Infliximab

Recent changes for Infliximab

Date
Field
Changed From
Changed To
5th April 2022
Alternative names

Inflectra, Remicade

Volume (Adults)

2 mL

Processing Instructions

After centifugation the serum must be aliquoted into the special aliquot tube provided and freeze.

Store at 4 C.

 

External Laboratory

South Western Area Pathology,Liverpool Hospital

Dorevitch Pathology

External Transport Instructions

Samples to be batched and sent  to SSWAPs frozen

Send to Testing Laboratory at 4 C

Frequency

Monthly

Weekly


 Recent changes for Itraconazole

Recent changes for Itraconazole

Date
Field
Changed From
Changed To
14th July 2022
Volume (Adults)

2 mL

5 mL

Minimum/Paediatric Volume

1 mL

Preferred Container Type

Lithium heparin no gel

EDTA

Processing Instructions

Spin, separate and freeze plasma.

Spin and separate plasma.

External Laboratory

Women's & Children's Hospital (Adelaide)

Alfred Hospital

External Transport Instructions

Packed by certified personnal - transport frozen.

Results : 08-81617000, 08-81616732.

Frequency

Monthly

Twice weekly

Notes

Dose information should be provided and length of therapy must be >5 days.


 Recent changes for Karyotype/Microarray - TISSUE

Recent changes for Karyotype/Microarray - TISSUE

Date
Field
Changed From
Changed To
6th March 2022
Assay name

Cytogenetics / Karyotype - TISSUE

Karyotype/Microarray - TISSUE


 Recent changes for Kleihauer stain

Recent changes for Kleihauer stain

Date
Field
Changed From
Changed To
8th August 2023
Alternative names

Kleihauer test

Kleihauer test, FMH (feto-maternal Haemorrhage)

Request Group

KL

FMH


 Recent changes for Lamotrigine

Recent changes for Lamotrigine

Date
Field
Changed From
Changed To
14th July 2022
Alternative names

Lamictal

Volume (Adults)

2 mL

3 mL

Minimum/Paediatric Volume

1 mL

3 mL

Processing Instructions

Spin aliquot plasma. (Send either plasma or whole blood)

Spin & aliquot plasma

External Laboratory

Australian Clinical Labs (formerly Healthscope)

Alfred Hospital


 Recent changes for Linezolid

Recent changes for Linezolid

Date
Field
Changed From
Changed To
14th July 2022
Volume (Adults)

2 mL

3 mL

Minimum/Paediatric Volume

3 mL

Optional Container Type

Lithium heparin no gel

Collection & Request Instructions

Deliver to laboratory ASAP.

A separate unopened specimen is required for this test.

Processing Instructions

Spin, separate and freeze plasma.

External Laboratory

Sydpath Pathology, St Vincent's Hospital NSW

Alfred Hospital

External Transport Instructions

Send at 4 C.

Frequency

As required

Weekly

Method

Results: Clinical Pharmacology on 02 8382 9184.


 Recent changes for Measles Serology

Recent changes for Measles Serology

Date
Field
Changed From
Changed To
18th January 2023
Laboratory

Microbiology

Biochemistry

Request Group

SMEA

MEA

Frequency

Daily

Twice weekly


 Recent changes for Methotrexate

Recent changes for Methotrexate

Date
Field
Changed From
Changed To
1st February 2023
Laboratory

Referred Test

Biochemistry

Collection & Request Instructions

Send ASAP (within 24 hrs, 7 days a week). Contact reference laboratory prior to sending the sample.

Testing performed on an as needs basis. 

External Laboratory

Peter MacCallum Cancer Institute

Method

Hand deliover samples to Biochemistry DO NOT load onto the 8100


 Recent changes for Mumps Serology

Recent changes for Mumps Serology

Date
Field
Changed From
Changed To
18th January 2023
Laboratory

Microbiology

Biochemistry

Request Group

SMUM

MUM

Frequency

Daily

Twice weekly


 Recent changes for Muscle Biopsy

Recent changes for Muscle Biopsy

Date
Field
Changed From
Changed To
2nd February 2024
Collection & Request Instructions

Note: This is an URGENT send out test.

Laboratory hours are 9am - 3pm, Monday - Friday.

Specimen must arrive at Alfred Pathology by 4pm.

 

Note: This is an URGENT send out test.

Laboratory hours are 9am - 5pm, Monday - Friday.

Specimens need to be delivered to Leve4 Specimen Reception by 3pm as the specimen must arrive at Alfred Pathology by 4pm.

 

30th December 2022
Collection & Request Instructions

Note: This is an URGENT send out test.

Laboratory hours are 9am - 3pm, Monday - Friday.

 

Note: This is an URGENT send out test.

Laboratory hours are 9am - 3pm, Monday - Friday.

Specimen must arrive at Alfred Pathology by 4pm.

 

External Transport Instructions

Place the specimen in an esky with ice cubes and dispatch immediately.

Place the specimen in an esky with ice cubes and dispatch immediately. Must arrive at Alfred by 4pm.

30th December 2022
Collection & Request Instructions

Note: This is an URGENT send out test.

Laboratory hours are 9am - 5pm, Monday - Friday.

 

Note: This is an URGENT send out test.

Laboratory hours are 9am - 3pm, Monday - Friday.

 


 Recent changes for Plasma Free Haemoglobin

Recent changes for Plasma Free Haemoglobin

Date
Field
Changed From
Changed To
27th June 2023
Assay name

Plasma Free Heamoglobin

Plasma Free Haemoglobin

Collection & Request Instructions

Samples must be hand delivered to the lab to avoid shaking, which will falsely raise the plasma free-haemoglobin. Normal operating plasma free-haemoglobin level is < 0.10 g/dL. This is performed 24-hourly whilst on ECMO (routinely taken with the daily blood samples) according to clinical need/ECMO clinician direction.

Samples are taken by passively filling a 10mL syringe carefully from the intra-arterial line access port. Do not connect to a negative pressure “vacutainer” for sampling. The plasma free-haemoglobin collection tube is filled from the syringe after removing the lid, gently injecting blood from the syringe and then replacing the lid. No needles are required.

27th June 2023
Preferred Specimen Type

Plasma

Serum

Preferred Container Type

Serum tube with gel

Lithium heparin no gel


 Recent changes for Procalcitonin

Recent changes for Procalcitonin

Date
Field
Changed From
Changed To
17th November 2022
Method

All PCT requests must be discussed with the Chemical Pathologist upon receipt in lab, regardless of time of day or day of week. If urgent/Taxi requiring is determined, result must be phoned to the clinician upon receipt - High Risk Result.

17th August 2022
Method

All PCT requests must be discussed with the Chemical Pathologist upon receipt in lab, regardless of time of day or day of week. If urgent/Taxi requiring is determined, result must be phoned to the clinician upon receipt - High Risk Result.

27th July 2022
Laboratory

Biochemistry

Referred Test

Volume (Adults)

0.5 mL

External Laboratory

Royal Childrens Hospital


 Recent changes for Prothrombin Time

Recent changes for Prothrombin Time

Date
Field
Changed From
Changed To
25th March 2022
Collection & Request Instructions

Filled to label marker and mixed by inversion.

Patient Information - Warfarin Management

 

Filled to label marker and mixed by inversion.

Patient Information - Warfarin Management-English
Patient Information - Warfarin Management-Greek
Patient Information - Warfarin Management-Simplified Chinese

 

 


 Recent changes for Q Fever Serology

Recent changes for Q Fever Serology

Date
Field
Changed From
Changed To
23rd November 2023
External Laboratory

ICPMR (Institute of Clinical Pathology and Medical Research)

Method

 

Contact VIDRL 9342 2649 / 9342 2650

Test performed by Institute of Clinical Pathology and Medical Research (ICPMR), NSW

8th February 2023
External Laboratory

Victorian Infectious Diseases Reference Laboratory (V.I.D.R.L)


 Recent changes for Quantiferon TB-Gold

Recent changes for Quantiferon TB-Gold

Date
Field
Changed From
Changed To
13th September 2023
Alternative names

QTFN, Quantiferon TB Gold

Quantiferon, TB Gold, QTFN

Frequency

Twice weekly

Weekly


 Recent changes for RAST

Recent changes for RAST

Date
Field
Changed From
Changed To
23rd May 2023
Collection & Request Instructions

White top tube is the preferred sample.0.2ml of blood required for each allergen.

Blood collection: still can be done after skin allergy testing.

Omega-5-Gliadin, Alpha Gal and Cefaclor : serum send to Melbourne Pathology

IgG 96 Food Allergy test is not a RAST test  It is performed at  Australian Clinical Labs.This test is NOT rebatable and costs approx  $260.

White top tube is the preferred sample.0.2ml of blood required for each allergen.

Blood collection: still can be done after skin allergy testing.

RAST for Omega-5-Gliadin to be sent to Alfred Pathology, Alpha Gal to be sent to Melbourne Pathology.                               Cefaclor and ARAh2 to be sent to Dorevitch Pathology 

IgG 96 Food Allergy test is not a RAST test  It is performed at  Australian Clinical Labs.This test is NOT rebatable and costs approx  $260.

Processing Instructions

Omega-5-Gliadin: send to Melborne Pathology

IgG 96 Food Allergy test is not a RAST test  It is performed at  Australian Clinical Labs.This test is NOT rebatable and costs approx  $260.

 

IgG 96 Food Allergy test is not a RAST test  It is performed at  Australian Clinical Labs.This test is NOT rebatable and costs approx  $260.

 

Method

RAST for Omega-5-Gliadin to be sent to Alfred Pathology, Alpha Gal to be sent to Melbourne Pathology.                               Cefaclor and ARAh2 to be sent to Dorevitch Pathology 


 Recent changes for Red Cell Antigen Genotyping

Recent changes for Red Cell Antigen Genotyping

Date
Field
Changed From
Changed To
9th May 2022
Collection & Request Instructions

Minimum volume of 4 ml required - this specimen cannot be used for any other purpose.

A completed "ARCBS Red Cell Reference Form" must accompany each sample sent for analysis.

This form, which can be completed online and subsequently printed to be sent with the sample can be found by clicking here 

Minimum volume of 4 ml required - this specimen cannot be used for any other purpose.

A completed "ARCBS Red Cell Reference Form" must accompany each sample sent for analysis.

This form, which can be completed online and subsequently printed to be sent with the sample can be found by clicking here 

Processing Instructions

Specimen will arrive in department at RT.  Please keep at 4 degrees prior to transport.

Specimen will arrive in department at RT.  Please keep at 4 degrees prior to transport.

External Transport Instructions

Transport at 4 degrees

Transport at 4 degrees


 Recent changes for Renin - plasma

Recent changes for Renin - plasma

Date
Field
Changed From
Changed To
22nd February 2024
Collection & Request Instructions

 

Collection Requirements for both or either tests

Collect into 4 mL EDTA tubes at Room Temperature. Do not collect on ice

If both Renin and Aldosterone are requested together, they can be collected into the same EDTA tube

In the laboratory, spin and separate the plasma immediately. Plasma is stable at Room Temperature if analysis is performed on the same day.

Renin must be frozen at -80 C. Transport plasma at room temperature (Maroondah and Angliss) until it can be placed in -80 C Freezer.(Must be done within 3-4 hors).

Add-on tests for Renin and Aldosterone should not be done on samples which have been previously collected or stored at 4 C.

Renin will not be collected at Healesville Hospital and Yarra Ranges Health Collection Rooms.

 

Collection Requirements for both or either tests

Collect into 4 mL EDTA tubes at Room Temperature. Do not collect on ice

If both Renin and Aldosterone are requested together, they can be collected into the same EDTA tube

In the laboratory, spin and separate the plasma immediately. Plasma is stable at Room Temperature if analysis is performed on the same day.

Renin must be frozen at -80 C. Transport plasma at room temperature (Maroondah and Angliss) until it can be placed in -80 C Freezer.(Must be done within 3-4 hours).

Add-on tests for Renin and Aldosterone should not be done on samples which have been previously collected or stored at 4 C.

Renin will not be collected at Healesville Hospital and Yarra Ranges Health Collection Rooms.

Samples from Angliss and Maroondah must be collected within business hours to allow transport to Box Hill via courier.

23rd May 2023
Laboratory

Referred Test

Biochemistry

External Laboratory

Austin Pathology


 Recent changes for Respiratory Virus PCR

Recent changes for Respiratory Virus PCR

Date
Field
Changed From
Changed To
14th February 2023
Method

The following specimens will be referred for testing-

Sputum, Bronchial Washings, Bronchoalveolar Lavages - VIDRL

Swab specimens for Respiratory Multiplex PCR will be referred to Monash Molecular Laboratory

 

The following specimens will be referred for testing-

Sputum, Bronchial Washings, Bronchoalveolar Lavages - VIDRL

As of Feb 2023 swab specimens for Respiratory Multiplex PCR will be tested in-house.

 

31st August 2022
Preferred Specimen Type

Nasopharyngeal swab
Nasopharyngeal Aspirate

Nasopharyngeal swab
NTS - nose & throat swab
Nasopharyngeal Aspirate

Optional Specimen Type

Sputum
Bronchial Washings
Broncho-alveolar Lavage

Preferred Container Type

Viral Transport Media- Nasopharyngeal
Respiratory aspiration pot

Viral Transport Media- Nasopharyngeal
Respiratory aspiration pot
respiratory PCR requirements

Collection & Request Instructions

Please note these swabs are available from stores.

Search for: VIRAL TRANSPORT MEDIA - NASOPHARYNGEAL

Pathology do not keep a stock of these swabs. Emergency departments and wards should manage a supply of these swabs according to demand.

 

 

Please note these swabs are available from stores.

Pathology do not keep a stock of these swabs. Emergency departments and wards should manage a supply of these swabs according to demand.

 

 

Method

All tests for respiratory virus PCR are considered urgent and must be delivered to the laboratory ASAP.

ICU/Oncology patients testing negative for Flu A/B and RSV  will be referred to Monash for respiratory virus panel. This includes Influenza A virus, Influenza B virus, Respiratory Syncitial Virus, Picornavirus (RV/EV), Parainfluenza viruses 1,2 and 3, Adenovirus, Human Metapneumovirus, Bordetella pertussis, Bordetella parapertussis, Mycoplasma pneumoniae, Legionella species, Chlamydiaceae.

The following specimens will be referred for testing-

Sputum, Bronchial Washings, Bronchoalveolar Lavages

Specimens for Respiratory Multiplex PCR will be referred to Monash Molecular Laboratory

Contact Monash Pathology 9594 4532 or if no answer try Bacteriology 9594 4565 as there is someone rostered 24/7

 

The following specimens will be referred for testing-

Sputum, Bronchial Washings, Bronchoalveolar Lavages - VIDRL

Swab specimens for Respiratory Multiplex PCR will be referred to Monash Molecular Laboratory

 

Notes

This PCR will detect and identify: Influenza A virus, Influenza B virus and Respiratory syncytial virus (RSV).

Transport at room temperature to the Laboratory

Testing is performed at Box Hill, Angliss, and Maroondah.

Testing  Lab

Testing hours

TAT (from receipt in lab)

All sites

 

Available 24/7 

1 hr

Samples received after hours may have a longer TAT due to other urgent critical pathology testing occurring.

This PCR will detect and identify: Influenza A virus, Influenza B virus and Respiratory syncytial virus (RSV).

Transport at room temperature to the Laboratory

Testing is performed at Box Hill, Angliss, and Maroondah.

Testing  Lab

Testing hours

TAT (from receipt in lab)

All sites

 

Available 24/7 

6-12 hrs routine

2 hours (rapid approval form required)

DO NOT send samples in the Pathology Chute

For Rapid testing from Emergency Department - swab must be accompanied by signed Rapid Test Authorisation Form.

DO NOT call the laboratory for results/enquiries. Inpatient tests will be prioritised and results available on EMR ASAP.

Sputum samples are referred to an external laboratory - results will be delayed.


 Recent changes for Respiratory Virus PCR - Extended

Recent changes for Respiratory Virus PCR - Extended

Date
Field
Changed From
Changed To
14th February 2023
Preferred Container Type

Viral Transport Media - Nasopharyngeal

Viral Transport Media - Nasopharyngeal
respiratory PCR requirements
Viral Transport Media- Nasopharyngeal

14th February 2023
Preferred Specimen Type

NTS - nose & throat swab
Swab

NTS - nose & throat swab
Respiratory samples


 Recent changes for Rickettsia spp. Screen Serology

Recent changes for Rickettsia spp. Screen Serology

Date
Field
Changed From
Changed To
23rd November 2023
Frequency

Weekly

As required

Method

Test performed by Institute of Clinical Pathology and Medical Research (ICPMR), NSW

Notes

 

Tests for Total Ab, IgM  response to Spotted fever, Typhus and Scrub Typhus groups.

 

Tests for serology response to Spotted fever, Typhus and Scrub Typhus groups.

 

8th February 2023
External Laboratory

Victorian Infectious Diseases Reference Laboratory (V.I.D.R.L)


 Recent changes for Soluscopes for sterility testing

Recent changes for Soluscopes for sterility testing

Date
Field
Changed From
Changed To
23rd November 2023
Collection & Request Instructions

Soluscope should be sent to laboratory in sterile container

23rd November 2023
Preferred Specimen Type

Culture

not required

23rd November 2023
Preferred Specimen Type

not required

Culture

Preferred Container Type

Not required

Sterile Jar

23rd November 2023
Frequency

As required


 Recent changes for Stratify JCV

Recent changes for Stratify JCV

Date
Field
Changed From
Changed To
23rd May 2023
Processing Instructions

Samples are be aliquoted and frozen in the barcoded tube supplied in the JCV kit

Notes

Samples are be aliquoted and frozen in the barcoded tube supplied in the JCV kit


 Recent changes for Thiopurine Metabolites

Recent changes for Thiopurine Metabolites

Date
Field
Changed From
Changed To
3rd March 2023
Preferred Container Type

Lithium heparin no gel
EDTA

See Instructions

25th August 2022
Collection & Request Instructions

Collect 1 x 4 ml Lithium Heparin tube and

1 x 4ml EDTA tube.

FBE must be requested with this test as a RCC is required for calculation of results

DO NOT SPIN.

Keep at Room Temp.

 

Collect 1 x 4 ml Lithium Heparin tube and

2 x 4ml EDTA tube.

FBE must be requested with this test as a RCC is required for calculation of results

DO NOT SPIN.

Keep at Room Temp.

 

23rd August 2022
Collection & Request Instructions

Collect 1 x 4 ml Lithium Heparin tube and 1 x 4ml EDTA tube.

FBE must be requested with this test as a RCC is required for calculation of results

DO NOT SPIN.

Keep at Room Temp.

 

Collect 1 x 4 ml Lithium Heparin tube and

1 x 4ml EDTA tube.

FBE must be requested with this test as a RCC is required for calculation of results

DO NOT SPIN.

Keep at Room Temp.

 

23rd August 2022
Request Group

TMETAB

Preferred Container Type

EDTA

Lithium heparin no gel
EDTA

Collection & Request Instructions

Transport specimen at Room Temperature to lab

Collection & Request Instructions

Collect 2x4ml EDTA blood. DO NOT SPIN

Keep at 4 deg C, do not freeze.

The metabolites are stable for upto 4 days at 4 degress.

Collect 1 x 4 ml Lithium Heparin tube and 1 x 4ml EDTA tube.

FBE must be requested with this test as a RCC is required for calculation of results

DO NOT SPIN.

Keep at Room Temp.

 

Processing Instructions

Keep samples at Room Temperature.

Do Not Spin. Whole Blood required

External Laboratory

St Vincents Hospital

Frequency

Weekly

Notes

Imuran is a trade name for azathioprine

The Thiopurine metabolites (6-TGN & 6-MMP) are used as a means of estimating the toxicity and efficacy of azathioprine treatment.

This test is performed by the Gastroenerology department. ( Phone No.9895 4632 )

The cost for the test is not covered by Medicare

Eastern Health patients:6TGN 6MMP Thiopurine Metabolites Testing $75

All other patients: 6TGN 6MMP Thiopurine Metabolites Testing $145

Imuran is a trade name for azathioprine

The Thiopurine metabolites (6-TGN & 6-MMP) are used as a means of estimating the toxicity and efficacy of azathioprine treatment.

 

 


 Recent changes for Tissue release from Anatomical Pathology

Recent changes for Tissue release from Anatomical Pathology

Date
Field
Changed From
Changed To
6th March 2022
Laboratory

Unassigned

Anatomical Pathology

6th March 2022
External Transport Instructions

https://pathology.easternhealth.org.au/handbook/downloads/anatomical-pathology/Tissue%20Release%20Consent%20Form.doc


 Recent changes for Toxoplasma serology

Recent changes for Toxoplasma serology

Date
Field
Changed From
Changed To
18th January 2023
Request Group

STOX

TOX


 Recent changes for Transthyretin

Recent changes for Transthyretin

Date
Field
Changed From
Changed To
13th September 2022
Processing Instructions

The serum must be aliquoted and frozen. Transthyretin is only stable for 3 days at 4-8C .

The serum must be aliquoted and frozen. Transthyretin is only stable for 3 days at 4-8C .

External Laboratory

Monash Pathology


 Recent changes for Typhoid

Recent changes for Typhoid

Date
Field
Changed From
Changed To
31st January 2023
Alternative names

enteric fever, Typhoid fever

enteric fever, typhoid fever

31st January 2023
Preferred Container Type

Blood Culture

Blood Culture (Adult)
Blood Culture (Paediatric)


 Recent changes for Vitamin B1

Recent changes for Vitamin B1

Date
Field
Changed From
Changed To
18th November 2022
External Laboratory

Australian Clinical Labs (formerly Healthscope)

St Vincents Hospital


 Recent changes for Vitamin B6

Recent changes for Vitamin B6

Date
Field
Changed From
Changed To
18th November 2022
External Laboratory

Australian Clinical Labs (formerly Healthscope)

St Vincents Hospital


 Recent changes for Vitamin C

Recent changes for Vitamin C

Date
Field
Changed From
Changed To
31st March 2022
Volume (Adults)

2 mL

1 mL

Minimum/Paediatric Volume

1 mL

Processing Instructions

Spin, separate, wrap in foil and freeze ASAP.

Transport frozen. Contact Gribbles to arrange courier.

Spin, separate, wrap in foil and freeze ASAP.

Transport frozen. 

External Laboratory

Australian Clinical Labs (formerly Healthscope)

Alfred Hospital

Frequency

Weekly

Fortnightly

Pathology Handbook

Test & Collection Guide

Feedback

Eastern Health Pathology always welcomes the opportunity to improve the service we provide you.