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Recent Changes - Test & Collection Guide

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 Recent changes for Aldosterone - urine

Recent changes for Aldosterone - urine

Date
Field
Changed From
Changed To
4th February 2019
Preferred Container Type

24 hour urine

24-hour urine - ACID bottle

Collection & Request Instructions

24-hour urine collection in plain bottle

Keep refrigerated during collection and send to laboratory immediately on completion

Patient Collection Instructions

24-hour urine collection in acid bottle

Keep refrigerated during collection and send to laboratory immediately on completion

Patient Collection Instructions

Method

24 hour urine, plain bottle. A 50 mL aliquot is sent with the recorded total 24 hrs volume.

An extra 50 mL aliquot needs to be stored.

24 hour urine, acid bottle. A 50 mL aliquot is sent with the recorded total 24 hrs volume.

An extra 50 mL aliquot needs to be stored.


 Recent changes for Alpha Foetoprotein

Recent changes for Alpha Foetoprotein

Date
Field
Changed From
Changed To
17th January 2020
Collection & Request Instructions

Clinical notes must be completed

If pregnant female, please state pregancy in weeks

AFP is useful in monitoring some testicular germ cell tumours and primary hepatocelluar carcnioma. After treatment, eg. orchidectomy, levels fall with a half-life of approximately 45 days. Values > 500 mg/L are suggestive of malignancy

However 50% of all malignancies have values < 500 mg/L. AFP can also be raised in a variety of non-malignant liver disorders, where levels are usually < 100 mg/L.

Clinical notes must be completed

If pregnant female, please state pregancy in weeks

AFP is useful in monitoring some testicular germ cell tumours and primary hepatocelluar carcnioma. After treatment, eg. orchidectomy, levels fall with a half-life of approximately 45 days. Values > 500 ug/L are suggestive of malignancy

However 50% of all malignancies have values < 500 ug/L. AFP can also be raised in a variety of non-malignant liver disorders, where levels are usually < 100 ug/L.


 Recent changes for Alpha-1 Antitrypsin - phenotyping

Recent changes for Alpha-1 Antitrypsin - phenotyping

Date
Field
Changed From
Changed To
18th June 2019
Frequency

Monthly


 Recent changes for Aluminium

Recent changes for Aluminium

Date
Field
Changed From
Changed To
12th February 2020
Collection & Request Instructions

 

LAB STAFF NOTE : The Reference Lab will only receive aliquots which are sent in a metal-free aliquot tube (see container picture above).  Sample must be decanted by pour-off (do not use plastic pipettes). 

 

LAB STAFF NOTE : The Reference Lab will only receive aliquots which are sent in a metal-free aliquot tube (see container picture above).  Sample must be decanted by pour-off (do not use plastic pipettes). 

7th February 2020
Collection & Request Instructions

The Reference Lab will only receive aliquots which are sent in a metal-free aliquot tube (see container picture above).  Sample must be decanted by pour-off (do not use plastic pipettes). 

 

LAB STAFF NOTE : The Reference Lab will only receive aliquots which are sent in a metal-free aliquot tube (see container picture above).  Sample must be decanted by pour-off (do not use plastic pipettes). 

7th February 2020
Preferred Container Type

Trace metal tube ( Dark blue )

Trace Metal with Aliquot

Collection & Request Instructions

Do Not spin - Transport at 40C

The Reference Lab will only receive aliquots which are sent in a metal-free aliquot tube (see container picture above).  Sample must be decanted by pour-off (do not use plastic pipettes). 


 Recent changes for Amiodarone

Recent changes for Amiodarone

Date
Field
Changed From
Changed To
13th February 2019
Collection & Request Instructions

Pre-dose serum is required.

Trough level - collect at least 6-8 hours after last dose.

Trough level - collect at least 6-8 hours after last dose.

 

Processing Instructions

Desethylamiodarone is the major metabolite of Amiodarone

Spin, aliquot and freeze plasma.

External Laboratory

St Vincents Hospital

Royal Brisbane & Womens Hospital


 Recent changes for Amyloid A

Recent changes for Amyloid A

Date
Field
Changed From
Changed To
17th September 2018
Processing Instructions

Spin, separate and freeze serum.

Transport frozen.

17th September 2018
Request Group

amla

AMLA

External Laboratory

The Children's Hospital at Westmead


 Recent changes for Angiotensin Converting Enzyme

Recent changes for Angiotensin Converting Enzyme

Date
Field
Changed From
Changed To
25th March 2019
Collection & Request Instructions

Transport ON ICE IMMEDIATELY to lab

Collection & Request Instructions

 

 

Preferable to receive a fasting sample.

Transport to laboratory on ice or transport immediately to laboratory and contact the laboratory staff to expect the sample.

 


 Recent changes for Anti-Mitochondrial Antibodies

Recent changes for Anti-Mitochondrial Antibodies

Date
Field
Changed From
Changed To
22nd February 2019
Alternative names

Mitochondrial Antibodies

Mitochondrial Antibodies, AMA


 Recent changes for Atypical Pneumonia Serology

Recent changes for Atypical Pneumonia Serology

Date
Field
Changed From
Changed To
21st August 2018
Method

Contact VIDRL 9342 2649/9342 2650

Use comment APS when acute sera received and store pending receipt of convalescent sample.

Contact VIDRL 9342 2649/9342 2650

Notes

May 1st to September 30th - Mycoplasma, Legionella, Chlamydia & Influenza serology performed and Q fever when requested.  Influenza serology not performed routinely from 1st October to 30th April.

Paired sera (acute & convalescent) required (collected 2-3 weeks apart). Specimens will not be sent until convalescent specimen received. The exception is Mycoplasma pneumoniae - if < 40 y.o. testing will be performd on initial specimen.

 

Paired sera (acute & convalescent) required (collected 2-3 weeks apart). Specimens will not be sent until convalescent specimen received.

 


 Recent changes for Beta - 2-Transferrin, Asialo - Fluid

Recent changes for Beta - 2-Transferrin, Asialo - Fluid

Date
Field
Changed From
Changed To
19th May 2020
Volume (Adults)

1.0 mL

Minimum/Paediatric Volume

0.2 mL

Collection & Request Instructions

Wicks and Gauze will not be accepted.

Notes

The presence of asialotransferrin in a fluid indicates that it is, or contains, CSF.

The presence of asialotransferrin in a fluid indicates that it is, or contains, CSF.

Pathologist approval required.


 Recent changes for Blood Cultures - Adult

Recent changes for Blood Cultures - Adult

Date
Field
Changed From
Changed To
7th October 2019
Notes

Refer to Objectify Poliy # 2086. Collection of Blood Cultures

 

 Refer to Objectify Poliy # 2086. Collection of Blood Cultures

 

7th October 2019
Notes

Blood Culture Collection Instructions

Blood Culture Clinical Information

Refer to Objectify Poliy # 2086. Collection of Blood Cultures

 


 Recent changes for Blood Cultures - Children and Neonates

Recent changes for Blood Cultures - Children and Neonates

Date
Field
Changed From
Changed To
7th October 2019
Notes

Blood Culture Collection Instructions

Blood Culture Clinical Information

 Refer to Objectify Policy # 2086 Collection of Blood Cultures


 Recent changes for Bone Marrow Examination

Recent changes for Bone Marrow Examination

Date
Field
Changed From
Changed To
23rd October 2018
Collection & Request Instructions

Bont marrow trephine requires at least 4 hours fixation prior to processing. Please consider this for an Urgent trephine result.

Bone Marrow Collection Instructions

Bont marrow trephine requires at least 4 hours fixation prior to processing. Please consider this for an Urgent trephine result.

 


 Recent changes for C-Reactive Protein (high sensitive)

Recent changes for C-Reactive Protein (high sensitive)

Date
Field
Changed From
Changed To
12th July 2019
Laboratory

Blood Bank

Referred Test

Processing Instructions

CRP-hs is run on a weekly basis in the Box Hill lab.  Specimens should be frozen at -20 within 24 hours of collection.

External Laboratory

St Vincents Hospital

External Transport Instructions

If transporting within 24 hours of collection, 4 deg transport is acceptable.  Otherwise, aliquot should be frozen.

Frequency

Weekly

Daily


 Recent changes for CADASIL-Notch 3 gene mutation

Recent changes for CADASIL-Notch 3 gene mutation

Date
Field
Changed From
Changed To
7th November 2019
External Laboratory

Neurogenetics Unit, Level 2, Royal Perth Hospital

Pathwest Lab Med WA

Frequency

As required

Method

Packed by certified personnel

Dr M Davis, Neurogenetics Unit, DPT of Anatomical Pathology, Level 2, North Block, RPA, Wellington Street, PERTH, WA 6000

(Ph) 08-92243136.

Packed by certified personnel

Dr M Davis, Diagnostic Genomics, Level 2

C/O Specimen Reception Ground Floor PP Building

QEII Medical Centre Hospital Avenue

Nedlands WA 6009 AUSTRALIA

(Ph) 08-63834243

Notes

Defects in NOTCH3 are the cause of cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL) . CADASIL causes a type of stroke and dementia of which key features include recurrent subcortical ischemic events and vascular dementia. The disorder affects relatively young adults of both sexes. Mutations affect highly conserved cysteine residues within epidermal growth factor (EGF)-like repeat domains in the extracellular part of the receptor.

Defects in NOTCH3 are the cause of cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL) . CADASIL causes a type of stroke and dementia of which key features include recurrent subcortical ischemic events and vascular dementia. The disorder affects relatively young adults of both sexes. Mutations affect highly conserved cysteine residues within epidermal growth factor (EGF)-like repeat domains in the extracellular part of the receptor.


 Recent changes for Cadmium

Recent changes for Cadmium

Date
Field
Changed From
Changed To
12th February 2020
Preferred Container Type

Trace Metal with Aliquot

Trace metal tube ( Dark blue )

Notes

Transport specimen at Room Temperature to lab
Sample haemolysis may make this test unreportable

 

LAB STAFF NOTE : The Reference Lab will only receive aliquots which are sent in a metal-free aliquot tube (see container picture above).  Sample must be decanted by pour-off (do not use plastic pipettes). 

Transport specimen at Room Temperature to lab
Sample haemolysis may make this test unreportable

 

 


 Recent changes for Candida auris screen

Recent changes for Candida auris screen

Date
Field
Changed From
Changed To
15th November 2019
Alternative names

Candida screen

Candida screen, Candida auris


 Recent changes for Cerebrospinal Fluid - Cytology

Recent changes for Cerebrospinal Fluid - Cytology

Date
Field
Changed From
Changed To
31st May 2019
Notes

out of hours CSF samples for cytology can be stored in the refrigerator for routine testing

Out of hours CSF samples for routine testing can be stored in the AP refrigerator.

 

Out of hours CSF samples for routine testing can be stored in the AP refrigerator.

 

31st May 2019
Collection & Request Instructions

CSF must be delivered to laboratory immediately; cellular degeneration occurs within 30 minutes.

The sample with the least amount of blood is prefered for Cytology.

Collect CSF into three separate, sterile, screw-capped tubes provided (labelled 1,2 & 3)

Tube 1 is for Biochemistry (for Protein and Glucose) 0.5ml (10 drops) required

Tube 2 for Cytology 1.0ml (20 drops)  and/or Flow Cytometry (6ml)

For Flow Cytometry a minimum of 6ml is required  in Tube 2

For Flow Cytometry and Cytology a minimum of 7ml is required in Tube 2    

Tube 3 for Microbiology upto 10ml (Full tube)

Xanthochromia investigation requires a 4th tube containing 1ml of CSF .

The sample with the least amount of blood is prefered for Cytology.

Collect CSF into three separate, sterile, screw-capped tubes provided (labelled 1,2 & 3)

Tube 1 is for Biochemistry (for Protein and Glucose) 0.5ml (10 drops) required

Tube 2 for Cytology 1.0ml (20 drops)  and/or Flow Cytometry (6ml)

For Flow Cytometry a minimum of 6ml is required  in Tube 2

For Flow Cytometry and Cytology a minimum of 7ml is required in Tube 2    

Tube 3 for Microbiology upto 10ml (Full tube)

Xanthochromia investigation requires a 4th tube containing 1ml of CSF.

External Transport Instructions

All out of hours specimens from Anglss and Maroondah should be sent to Box Hill ASAP for processing.

After hours cytology CSF can be prepared by microbiology staff. In the event microbiology staff are not in attendance and will not be called in, please call the Anatomical Pathology Scientist on call.

After hours cytology CSF can be prepared by microbiology staff. In the event microbiology staff are not in attendance please store in the AP fridge.

Method

CSF sample processing:

If Cytology/Flow Cytometry requested  : Tube 1-Biochemistry, Tube 2- Cytology/Flow Cytometry, Tube 3-Microbiology

If Cytology/Flow Cytometry not requested: Tube 1 and 3-Microbiology, Tube 2-Biochemistry

Any confusion between the terms cell count and cytology could be cleared up when the cell count is rung through to the requesting doctor by the scientist. At this point it could be determined whether cytology is a genuine request or not (all to be documented in F8 notes). The CSF can then be forwarded expediently to BHH for cytospin if required.

 

 

CSF sample processing:

If Cytology/Flow Cytometry requested  : Tube 1-Biochemistry, Tube 2- Cytology/Flow Cytometry, Tube 3-Microbiology

If Cytology/Flow Cytometry not requested: Tube 1 and 3-Microbiology, Tube 2-Biochemistry

Any confusion between the terms cell count and cytology could be cleared up when the cell count is rung through to the requesting doctor by the scientist. At this point it could be determined whether cytology is a genuine request or not (all to be documented in F8 notes).

 

 

Notes

out of hours CSF samples for cytology can be stored in the refrigerator for routine testing

Out of hours CSF samples for routine testing can be stored in the AP refrigerator.

 


 Recent changes for Chromium

Recent changes for Chromium

Date
Field
Changed From
Changed To
12th February 2020
Preferred Container Type

Trace metal tube ( Dark blue )

Trace Metal with Aliquot

Collection & Request Instructions

LAB STAFF NOTE : The Reference Lab will only receive aliquots which are sent in a metal-free aliquot tube (see container picture above).  Sample must be decanted by pour-off (do not use plastic pipettes). 


 Recent changes for Chromogranin A

Recent changes for Chromogranin A

Date
Field
Changed From
Changed To
12th September 2018
Request Group

CHRA/CHRMA

CHRA

External Laboratory

Sydney South West Pathology Service(RPA)

Peter MacCallum Cancer Institute

External Transport Instructions

Sample must be packed by IATA certified staff member.

Transport serum frozen on dry ice.

Samples will be sent to Peter MacCallum/Sydney South West Pathology (RPA).

Frequency

Monthly

Weekly


 Recent changes for Coagulation Studies

Recent changes for Coagulation Studies

Date
Field
Changed From
Changed To
10th April 2019
Notes

When blood is collected from an arterial line it is necessary to discard the first 20 mL of blood to clear any heparin from the line before collecting the blood for coagulation tests

When collection is by vacuum tube, the coagulation tube should be collected second to avoid artefactual shortening of coagulation assays due to trauma of venesection

Whe blood is collected for an APTT or Anti-Xa Assay for control of heparin or low molecular weight heparin therapy, it is essential that the citrate tube is sent immediately to the laboratory

Tests include : INR, APTT

Do not use 1 ml citrate tubes.

When blood is collected from an arterial line it is necessary to discard the first 20 mL of blood to clear any heparin from the line before collecting the blood for coagulation tests

When collection is by vacuum tube, the coagulation tube should be collected second to avoid artefactual shortening of coagulation assays due to trauma of venesection

Whe blood is collected for an APTT or Anti-Xa Assay for control of heparin or low molecular weight heparin therapy, it is essential that the citrate tube is sent immediately to the laboratory

 

Do not use 1 ml citrate tubes.

9th April 2019
Request Group

COAG

COAGP


 Recent changes for Coronavirus PCR

Recent changes for Coronavirus PCR

Date
Field
Changed From
Changed To
24th January 2020
Alternative names

extended panel PCR, MERS PCR, novel coronavirus PCR, Respiratory Virus NAD, SARS PCR

extended panel PCR, MERS PCR, novel coronavirus PCR, Respiratory Virus NAD, SARS PCR, novel Wuhan

24th January 2020
Alternative names

Respiratory Virus NAD, SARS PCR

Respiratory Virus NAD, SARS PCR, extended panel PCR, novel coronavirus PCR, MERS PCR

Request Group

RESPCR

RESPX


 Recent changes for CRE Screen

Recent changes for CRE Screen

Date
Field
Changed From
Changed To
1st August 2018
Collection & Request Instructions

Faeces is the preferred specimen. When not possible a rectal swab AND an inguinal swab should be taken.

Microbiology Specimen Collection Guide

Faeces is the preferred specimen. When not possible a rectal swab (with visible faeces on the swab)  AND an inguinal swab should be taken.

Microbiology Specimen Collection Guide


 Recent changes for Creutzfeldt-Jakob Disease

Recent changes for Creutzfeldt-Jakob Disease

Date
Field
Changed From
Changed To
11th October 2018
Method

NOTIFY REGISTRY THAT SAMPLE IS BEING SENT.

Transport on dry ice.

CSF : Test is run weekly. Samples received by 4 p.m. Friday will have the result issued by the end of the following week.

 Blood : Test for PRNP (Prion protein gene)

5 mLs in EDTA tube

Send to

The Australian National CJD Registry

Dept. of Pathology, Faculty of Medicine

The University of Melbourne

Melbourne Brain Centre (Kenneth Myer Building)

Cnr Genetics Lane & Royal Pde

Gate 11, Rear Loading Dock

Parkville , Victoria 3052

Phone 8344 1949

Fax 8349 5105

 

Link to request form Updated 14.6.18

https://www.florey.edu.au/uploads/content/CJD-FO-008_CSF_Specimen_Data_Sheet_with_14-3-3_and_Tau.pdf

NOTIFY REGISTRY THAT SAMPLE IS BEING SENT.

Transport on dry ice.

CSF : Test is run weekly. Samples received by 4 p.m. Friday will have the result issued by the end of the following week.

 Blood : Test for PRNP (Prion protein gene)

5 mLs in EDTA tube

Send to

The Australian National CJD Registry

Dept. of Pathology, Faculty of Medicine

The University of Melbourne

Melbourne Brain Centre (Kenneth Myer Building)

Cnr Genetics Lane & Royal Pde

Gate 11, Rear Loading Dock

Parkville , Victoria 3052

Phone 8344 1949

Fax 8349 5105

 


 Recent changes for Cyclosporin A

Recent changes for Cyclosporin A

Date
Field
Changed From
Changed To
6th May 2020
Method

PLEASE NOTE : CYCLOSPORIN REFERRALS MUST ONLY BE SENT TO THE AUSTIN - THEY MUSY NOT BE SENT TO THE ALFRED AS THEY USE A METHOD THAT DOES NOT CORRESPOND WITH THE EXPECTED REFEERNCE RANGES.

PLEASE NOTE : CYCLOSPORIN send to the Austin Biochemistry Department.

6th May 2020
Collection & Request Instructions

In post transplant patients a 2 hour post dose is the preferred specimen, please indicate dosing information on the request slip.

Specimen must arrive at the Box Hill laboratory before 1200 Mon-Fri.

Separate EDTA specimen required.

 

In post transplant patients a 2 hour post dose is the preferred specimen, please indicate dosing information on the request slip.

Specimen must arrive at the Box Hill laboratory before 1200 everyday.

Separate EDTA specimen required.

 


 Recent changes for Cystic Fibrosis - Genetic Testing

Recent changes for Cystic Fibrosis - Genetic Testing

Date
Field
Changed From
Changed To
19th October 2018
Collection & Request Instructions

Newborn/Infants: 1-2 ml EDTA

Children: 2-5 ml EDTA

Adults: 5-10 ml EDTA

Newborn/Infants: 1-2 ml EDTA

Children: 2-5 ml EDTA

Adults: 5-10 ml EDTA

Frequency

As required


 Recent changes for Cytogenetics - Tumour Tissue Block (Adult)

Recent changes for Cytogenetics - Tumour Tissue Block (Adult)

Date
Field
Changed From
Changed To
4th November 2018
Assay name

Cytogenetics - Tumour (Adult)

Cytogenetics - Tumour Tissue Block (Adult)


 Recent changes for Cytogenetics/Karyotyping

Recent changes for Cytogenetics/Karyotyping

Date
Field
Changed From
Changed To
4th November 2018
Assay name

SKIN BIOPSY - KARYOTYPE

Cytogenetics Tissue Non-Malignant (SKIN BIOPSY - KARYOTYPE)

Preferred Specimen Type

Biopsy

Tissue

Optional Specimen Type

Biopsy

Collection & Request Instructions

Refrigerate, send to lab upon completion

Collection & Request Instructions

https://handbook.monashpathology.org/index?testid=1507

https://handbook.monashpathology.org/index?testid=1507

Collect aseptically. Collect x2 skin punches from extant patients.

Collect approx 5mm square (with 2-3mm depth) skin biopsy from deceased patients.

Use RPMI (obtain from Pathology) for transport. Do not allow the sample to dry out.

External Transport Instructions

Send to Anatomical Pathology for send out to MMC.

Notes

Send to Pathology for send out to MMC.


 Recent changes for Disaccharidases, Maltase, Sucrase, Lactase and Isomaltase

Recent changes for Disaccharidases, Maltase, Sucrase, Lactase and Isomaltase

Date
Field
Changed From
Changed To
28th May 2019
Processing Instructions

Place the biopsy specimen in the centre of a piece of Parafilm about 3cm square, and fold the Parafilm over the specimen so that it forms an envelope around the specimen. Then place this in a capped plastic tube with an appropriate label. Lab Notes Store and transport frozen.

THE TISSUE SAMPLE MUST BE FROZEN IMMEDIATELY AFTER COLLECTION, AS FOLLOWS.
1. WRAP (FOLD) THE TISSUE SAMPLE IN PARAFILM FIRST.
2. THEN WRAP THE SAMPLE IN ALFOIL.
3. FREEZE THE SPECIMEN ASAP.
4. SEND ON DRY ICE

External Laboratory

Royal Childrens Hospital

Sullivan Nicolaides Pathology ( Via Melb Path)


 Recent changes for Familial mediterranean genetic screen

Recent changes for Familial mediterranean genetic screen

Date
Field
Changed From
Changed To
11th October 2019
Collection & Request Instructions

Room temperature

This test is not covered by Medicare.

Cost for testing is $250

.

Room temperature

This test is not covered by Medicare.

Cost for testing is $300.

 

Processing Instructions

Interstate courier- Room Temperature

Department Of Molecular Genetics, Children's Hospital at Westmead, Loading Dock 5, Redbank Road, Northmead, NSW 2152.

For results Tel: 02-98453244

Interstate courier- Room Temperature

Department Of Molecular Genetics, Children's Hospital at Westmead, Loading Dock 5, Redbank Road, Northmead, NSW 2152.

For results Tel: 02-98453244


 Recent changes for FISH for C-MYC, bcl-2 and bcl-6 on paraffin embedded tissue

Recent changes for FISH for C-MYC, bcl-2 and bcl-6 on paraffin embedded tissue

Date
Field
Changed From
Changed To
7th November 2018
Preferred Container Type

Slide & carrier

See Instructions

Collection & Request Instructions

A request slip indicating the clinical notes, prefered FISH probe if know and tissue type.

The follwing 2 micron sections of tissue on positively charged slides (as per immunohistochemistry), clearly labelled with patient name and section thickness must be sent:

a. 4 patient slides per probe. NOTE: For MYC testing a minimum of 6 slides are required. For >2 probes eg. "double hit" samples a minimum of 9 slides are required.

b. 1 H&E stained slide of the section intended for FISH with a marker indicating the optimal region for FISH - ie. the region wiht the most concentrated area of tumour.

A request slip indicating the clinical notes, prefered FISH probe if known and tissue type.

The follwing 2 micron sections of tissue on positively charged slides (as per immunohistochemistry), clearly labelled with patient name and section thickness must be sent:

a. 4 patient slides per probe. NOTE: For MYC testing a minimum of 6 slides are required. For >2 probes eg. "double hit" samples a minimum of 9 slides are required.

b. 1 H&E stained slide of the section intended for FISH with a marker indicating the optimal region for FISH - ie. the region with the most concentrated area of tumour.

See attached referal instruction sheet: http://pathology.easternhealth.org.au/handbook/downloads/anatomical-pathology/FISH_VCGS_instructions_2018.pdf

External Transport Instructions

Please deliver all specimes to:

Victorian Cancer Cytogenetics Service

St Vincent's Hospital Melbourne

Second Floor, Inpatient Services Building

41 Victoria Parade, Fitzroy, VIC, 3065.

T: 9288-4154   F:9288-4155

lynda.campbell@svhm.org.au or fran.omalley@svhm.org.au

Please deliver all specimes to:

Victorian Cancer Cytogenetics Service

St Vincent's Hospital Melbourne

Second Floor, Building A Destination A26

41 Victoria Parade, Fitzroy, VIC, 3065.

T: 9231 4154   F:9231 4155

meaghan.wall@svha.org.au or fran.omalley@svhm.org.au


 Recent changes for Free PSA

Recent changes for Free PSA

Date
Field
Changed From
Changed To
27th August 2019
Processing Instructions

Frozen

Frozen

External Transport Instructions

The Sample must be separated and Frozen. fPSA is only stable for 24 hrs Max. The total PSA must be performed before the chemical pathologist/senior scientist can determine if the request is appropriate.

The Sample must be separated and Frozen. fPSA is only stable for 24 hrs Max. The total PSA must be performed before the chemical pathologist/senior scientist can determine if the request is appropriate.


 Recent changes for GBS Screen

Recent changes for GBS Screen

Date
Field
Changed From
Changed To
1st August 2018
Collection & Request Instructions

The optimal specimen is a low vaginal & ano-rectal swab.

Maternal Group B Streptococcus in Pregnancy: Screening and Management Guideline

http://ehpolicies.eh.local:90/index.aspx?itemDetails=2398

GBS Collection Guide

http://pathology.easternhealth.org.au/handbook/downloads/microbiology/GBS_Information_for_Pregnant_Women.doc

Microbiology Collection Guide

 

The optimal specimen is a low vaginal & ano-rectal swab.

Maternal Group B Streptococcus in Pregnancy: Screening and Management Guideline

http://ehpolicies.eh.local:90/index.aspx?itemDetails=2398

GBS Collection Guide

http://pathology.easternhealth.org.au/handbook/downloads/microbiology/GBS_Information_for_Pregnant_Women.doc

Microbiology Collection Guide

 


 Recent changes for Gonococcal PCR

Recent changes for Gonococcal PCR

Date
Field
Changed From
Changed To
21st August 2018
Method

From the 15/1/2018 Chlamydia trachomatis PCR will be performed in Microbiology at BHH on male and female first stream urines and female enodocervial/vaginal swabs.

Tests are performed Monday, Wednesday and Friday


If confirmatory testing required specimens will be sent to VIDRL .

Refer to WORK-MI-88 for instructions on how to transfer urines and swabs to collection tubes.

20/08/2018 if a gel swab is received send sample to Microbiology (do not add a KIMMS) who will decide if testing can be performed.

From the 15/1/2018 Chlamydia trachomatis PCR will be performed in Microbiology at BHH on male and female first stream urines and female enodocervial/vaginal swabs.

Tests are performed Monday, Wednesday and Friday

If confirmatory testing required specimens will be sent to VIDRL .

Refer to WORK-MI-88 for instructions on how to transfer urines and swabs to collection tubes.


 Recent changes for Haemoglobin A1c

Recent changes for Haemoglobin A1c

Date
Field
Changed From
Changed To
5th October 2018
Request Group

A1CS

HBA1CP, A1CP


 Recent changes for Heinz Bodies

Recent changes for Heinz Bodies

Date
Field
Changed From
Changed To
8th October 2018
Frequency

Daily

As required

Notes

  • An FBE is usually performed concurrently
  • The presence of Heinz bodies indicates oxidant drug haemolysis, asplenia or unstable haemoglobin

An FBE is usually performed concurrently

The presence of Heinz bodies indicates oxidant drug haemolysis, asplenia or unstable haemoglobin


 Recent changes for Heparin-Induced Thrombocytopenia Syndrome Screen

Recent changes for Heparin-Induced Thrombocytopenia Syndrome Screen

Date
Field
Changed From
Changed To
5th September 2019
Laboratory

Blood Bank

Referred Test

Collection & Request Instructions

HITScreen/ELISA testing is only available after consultation with the Consultant Haematologist or Haematology Registrar.

HITScreen/ELISA testing (HITE) is only available after consultation with the Consultant Haematologist or Haematology Registrar.

21st January 2019
Collection & Request Instructions

Transport specimen at Room Temperature to lab

21st January 2019
Collection & Request Instructions

The DiaMed Hits screen will detect approx. 85%of HITS positive patients.

If clinical indicators are strong but the DiaMed test negative, a serum sample can be forwarded for ELISA testing (HITE) at Monash Medical Centre

ELISA testing is only available after consultation with the Consultant Haematologist or Haematology Registrar.

ELISA testing is only available after consultation with the Consultant Haematologist or Haematology Registrar.

Processing Instructions

Laboratory staff at Monash Medical Centre must be informed if the ELISA test is to be done urgently.

transport - serum frozen to MMC unless test is to be done same day.

(MMC Coagulation: 9594 3489)

Laboratory staff at Monash Medical Centre must be informed if the ELISA test is to be done urgently.

Transport - serum frozen to MMC.

(MMC Special Haematology: 9594 3490)

External Laboratory

Monash Pathology

Notes

Plain tube with gel

Keep at room temperature and send to laboratory immediately.   DO NOT FREEZE

Plain tube with gel


 Recent changes for Hepatitis D Serology

Recent changes for Hepatitis D Serology

Date
Field
Changed From
Changed To
21st August 2019
Request Group

HEPDAB & HEPDAG

HEPDAB


 Recent changes for Hereditary Amyloidosis

Recent changes for Hereditary Amyloidosis

Date
Field
Changed From
Changed To
22nd August 2019
Alternative names

TTR, FGA, APOA1, LYZ or Amyloidosis Gene

Volume (Adults)

Minimum/Paediatric Volume


 Recent changes for HMG-CoA Reductase

Recent changes for HMG-CoA Reductase

Date
Field
Changed From
Changed To
7th February 2020
Alternative names

3-Hydroxy-3-Methylglutaryl-coenzyme A Reductase), Anti-HMGCR, Autoantibodies to HMGCR

3-Hydroxy-3-Methylglutaryl-coenzyme A Reductase, Anti-HMGCR, Autoantibodies to HMGCR


 Recent changes for Holotranscobalamin

Recent changes for Holotranscobalamin

Date
Field
Changed From
Changed To
24th February 2020
Processing Instructions

HoloTC is only stable for 3 days at 2-8 C, hence if it is being stored at an Eastern Health Lab over the weekend, freeze and transport frozen.

External Transport Instructions

Please send sample at 4 degrees C. for weekend storage please freeze.

Frequency

Daily

12th February 2020
Laboratory

Referred Test

Biochemistry

External Laboratory

Alfred Hospital


 Recent changes for IgE, Total

Recent changes for IgE, Total

Date
Field
Changed From
Changed To
3rd December 2019
Laboratory

Referred Test

Biochemistry

External Laboratory

St Vincents Hospital


 Recent changes for Imatinib Level

Recent changes for Imatinib Level

Date
Field
Changed From
Changed To
4th April 2019
Request Group

IMAT

14th February 2019
Processing Instructions

Spin,aliquot plasma and freeze.

External Transport Instructions

Frozen sample to be packed by IATA trained personal.


 Recent changes for Isohaemagglutinin titre

Recent changes for Isohaemagglutinin titre

Date
Field
Changed From
Changed To
22nd May 2019
Request Group

ISOHGT

Processing Instructions

A Blood Group and Antibody screen should be performed by Eastern Health Pathology in conjunction with this request ( in order to check for other antibodies and allow reporting of isohaemagglutinin titre ). Blood samples should be held and transported at 4 deg C. Samples can be held ( at 4 deg C ) for up to 3 days before dispatch.

A Blood Group and Antibody screen should be performed by Eastern Health Pathology in conjunction with this request ( in order to check for other antibodies and allow reporting of isohaemagglutinin titre ). Blood samples should be held and transported at 4 deg C. Samples can be held ( at 4 deg C ) for up to 3 days before dispatch.

Method

Isohaemagglutinin titre may be requested in organ transplant patients as a base line test and at different times post transplant. The test refers to the titre of any of anti A and anti B in serum. Each individual produces different quantities of these antibodies. In Renal transplant patients ( given non ABO matched organs ) a rise from baseline isohaemagglutinin titre may prompt clinical intervention.

Isohaemagglutinin titre may be requested in organ transplant patients as a base line test and at different times post transplant. The test refers to the titre of any of anti A and anti B in serum. Each individual produces different quantities of these antibodies. In Renal transplant patients ( given non ABO matched organs ) a rise from baseline isohaemagglutinin titre may prompt clinical intervention.


 Recent changes for JAK2 Mutation Analysis

Recent changes for JAK2 Mutation Analysis

Date
Field
Changed From
Changed To
7th October 2019
Collection & Request Instructions

Sample is stable at Room Temperature for at least 24 Hours.

JAK2 is for differentiation of reactive VS clonal myeloproliferative disorders.

Collect a separate sample for this test.

This test is not Medicare rebatable. (Cost $44.00 incl GST)

 

Sample is stable at Room Temperature for at least 24 Hours.

JAK2 is for differentiation of reactive VS clonal myeloproliferative disorders.

Collect a separate sample for this test.

 

 


 Recent changes for Lamotrigine

Recent changes for Lamotrigine

Date
Field
Changed From
Changed To
31st December 2018
Preferred Specimen Type

Serum

Plasma

Preferred Container Type

Plain tube no gel

Lithium heparin no gel


 Recent changes for Leucocyte Lysomal Enzyme Analysis

Recent changes for Leucocyte Lysomal Enzyme Analysis

Date
Field
Changed From
Changed To
21st January 2020
Notes

Room temperature.

Please note this test id not covered by Medicare Rebate - see 'Ordering' above.

Room temperature.

Please note this test is not covered by Medicare Rebate - see 'Ordering' above.

21st January 2020
Notes

Room temperature.

This test is not covered by Medicare, cost will be $432.00 as of March 2016.

Room temperature.

Please note this test id not covered by Medicare Rebate - see 'Ordering' above.

21st January 2020
Assay name

White Cell Enzymes

Leucocyte Lysomal Enzyme Analysis

Alternative names

Lysosomal Enzymes

White Cell Enzymes

Volume (Adults)

4 mL

8 mL

Minimum/Paediatric Volume

6 mL

Notes

Room temperature.

This test is not covered by Medicare, cost will be $474.00 as of March 2016.

Room temperature.

This test is not covered by Medicare, cost will be $432.00 as of March 2016.


 Recent changes for Lignocaine

Recent changes for Lignocaine

Date
Field
Changed From
Changed To
8th January 2020
External Laboratory

Pacific Laboratory Medicine Services

Royal Brisbane & Womens Hospital

Frequency

Daily

As required


 Recent changes for MAG Antibody

Recent changes for MAG Antibody

Date
Field
Changed From
Changed To
31st January 2019
Alternative names

Anti-myelin associated glycoprotein antibodies

Request Group

MAGIFA

MAGAB

Frequency

Weekly

Method

Email Melb path before sending at  sendaways.department@mps.com.au to advise the samples are coming.

Email Melb path before sending at  sendaways.department@mps.com.au to advise the samples are coming.


 Recent changes for Mercury Urine

Recent changes for Mercury Urine

Date
Field
Changed From
Changed To
5th September 2019
Request Group

UHG24

UHG24/UHG

Collection & Request Instructions

Urine mercury concentration can be done on spot urine sample.

Patient Collection Instructions

Urine mercury concentration can be done on spot urine sample.(UHG)

Patient Collection Instructions

30th August 2019
Request Group

HGCRU

UHG24

Volume (Adults)

50 mL

External Laboratory

Sydney South West Pathology Service(RPA)

Alfred Hospital

Frequency

Daily


 Recent changes for Molecular Gene Mutation Pathology - EGFR, BRAF, KRAS, TCR, FISH

Recent changes for Molecular Gene Mutation Pathology - EGFR, BRAF, KRAS, TCR, FISH

Date
Field
Changed From
Changed To
26th August 2018
Assay name

Molecular Gene Mutation Pathology - EGFR, BRAF, KRAS, TCR

Molecular Gene Mutation Pathology - EGFR, BRAF, KRAS, TCR, FISH

Alternative names

BRAF, EGFR1, Epidermal Growth Factor Receptor, erbB1, HER1, KRAS

BRAF, EGFR1, Epidermal Growth Factor Receptor, erbB1, HER1, KRAS, FISH

26th August 2018
External Laboratory

Peter MacCallum Cancer Institute

St Vincents Hospital

External Transport Instructions

Paraffin blocks or paraffin embedded tissue sections on uncoated slides.

You can either fax us (FaxNo: +61 3 9656 1460 ) the request form along with a copy of the histology report and we will request the block/slides on your behalf

OR

You can send the completed form with along with:

1. A copy of the pathology report

2. 10 x 5μm unstained tumour sections (and 4 x 3μm on POS CHARGED slides for IHC if required) OR paraffin block.

 

 

 

Paraffin blocks or paraffin embedded tissue sections on uncoated slides.

 

 

 

 

Method

Send out test.

MOLECULAR PATHOLOGY (Level 1)

Peter MacCallum Cancer Centre (APA)

7 St Andrews Place

East Melbourne, VIC 3002

FAX: +61 3 9656 1460 Telephone: +61 3 9656

Send out test.

THE DEPARTMENT OF ANATOMICAL PATHOLOGY

LEVEL 2 MAIN BUILDING,

ST VINCENTS HOSPITAL

41 VICTORIA PDE,

FITZROY VIC 3065 Ph.: 03 9231 1049 Fax: 03 9231 4580

 


 Recent changes for MRSA Screen

Recent changes for MRSA Screen

Date
Field
Changed From
Changed To
1st August 2018
Collection & Request Instructions

Microbiology Specimen Collection Guide

 

31st July 2018
Preferred Container Type

Swab - red top, plain swab, wooden shaft

Swab - blue top (Amies transport media)

Notes

Use dry swab (not in transport media)

The transit of specimen from Angliss or Maroondah lab and out of working hour collection may cause the prolonged turnaround time

The transit of specimen from Angliss or Maroondah lab and out of working hour collection may cause the prolonged turnaround time


 Recent changes for Mumps Virus PCR

Recent changes for Mumps Virus PCR

Date
Field
Changed From
Changed To
3rd January 2020
Preferred Container Type

Swab - red top, plain swab, wooden shaft
Sterile Jar

Swab - red top, plain swab, wooden shaft
Swab - blue top (Amies transport media)
Swab - white top, plain swab, plastic shaft
UTM/Sterile pot


 Recent changes for Muscle Biopsy

Recent changes for Muscle Biopsy

Date
Field
Changed From
Changed To
14th May 2019
Preferred Container Type

Not required

Sterile Jar

Collection & Request Instructions

Please contact the laboratory in advance to ensure a muscle biopsy kit is readily available for collection.

Collection is as per kit instructions.

Note: This is an URGENT send out test.

Laboratory hours are 9am - 5pm, Monday - Friday.

 

Note: This is an URGENT send out test.

Laboratory hours are 9am - 5pm, Monday - Friday.

 

Processing Instructions

Call the Victorian Neuromuscular Laboratory for kits.

Contact: 9076 5448

Kits are to be stored in the fridge.

Prior to giving the kit to theatre staff, add 2mls of concentrated glutaraldehyde into 18mls of cacodylate buffer (red top container).

 

Notify the Victorian Neuromuscular Laboratory.

Contact: 9076 5448

 

External Transport Instructions

This is an URGENT send out test.

 

Place the specimen in an esky with ice cubes and dispatch immediately.

Method

Call taxi from the lab. Fill out cab voucher logbook.

Send to: Victorian neuromuscular Laboratory Service (VNLS), Alfred Pathology Service, Ground Floor, main Ward Block, Commercial Road, Melbourne VIC 3004.

Contact: 9076 3150

 

Send to: Victorian Neuromuscular Laboratory Service (VNLS), Alfred Pathology Service, Ground Floor, Main Ward Block, Commercial Road, Melbourne VIC 3004. Contact: 9076 3150

 


 Recent changes for Mycobacterium ulcerans PCR

Recent changes for Mycobacterium ulcerans PCR

Date
Field
Changed From
Changed To
23rd April 2019
Preferred Container Type

Sterile Jar
Swab - white top, plain swab, plastic shaft

Sterile Jar
Swab - white top, plain swab, plastic shaft
Swab - blue top (Amies transport media)


 Recent changes for Notifiable Infectious Diseases

Recent changes for Notifiable Infectious Diseases

Date
Field
Changed From
Changed To
4th September 2018
Collection & Request Instructions

Quick guide to notifying conditions in Victoria

https://www2.health.vic.gov.au/Api/downloadmedia/%7BF4C3B4BC-79C7-4BBF-8122-09093D98E2DC%7D

Go to this link for all other information on notifications and forms.

http://ideas.health.vic.gov.au/notifying.asp

Fax form to Dept.of Health Fax no. 1300 651 170

Guide to notifying conditions in Victoria

https://www2.health.vic.gov.au/public-health/infectious-diseases/notification-procedures


 Recent changes for Parathyroid Hormone Related Protein

Recent changes for Parathyroid Hormone Related Protein

Date
Field
Changed From
Changed To
2nd January 2019
External Laboratory

Pacific Laboratory Medicine Services

External Transport Instructions

Sample must be transported on Dry Ice and packed accoring to IATA regulations by certified personnel.

Lab Contact: 

PaLMS Endocrinology
RNSH

Cameron Wood ph 02 9926 4161

Notes

This test is not Medicare rebateable and must be

transported interstate frozen. The patient must not have

been taking calcium supplements or Vitamin D and must

have hypercalacemia with a suppressed PTH. Test must

be discussed with Chemical Pathologist or Senior Scientist. 

 

 


 Recent changes for Platelet Antigen Testing, Parental (for Neonatal Immune Thrombocytopenia)

Recent changes for Platelet Antigen Testing, Parental (for Neonatal Immune Thrombocytopenia)

Date
Field
Changed From
Changed To
31st July 2018
Collection & Request Instructions

This test is performed on the parents of the affected child.

Samples required are:

Mother : 10 mL in plain tube (no gel), 20 mL in ACD  

Father : 20 mL in ACD, 20 mL in EDTA

Please contact the laboratory before collecting these specimens

This test is performed on the parents of the affected child.

Samples required are:

Mother : 10 mL in plain tube (no gel), 20 mL in ACD  and 2x EDTA

Father : 20 mL in ACD, 2x EDTA

Please contact the laboratory before collecting these specimens, keep samples at room temperature.

Frequency

As required


 Recent changes for Platelet Function Analysis

Recent changes for Platelet Function Analysis

Date
Field
Changed From
Changed To
22nd August 2019
Volume (Adults)

3.5 mL

Minimum/Paediatric Volume

3.5 mL

Collection & Request Instructions

 PLEASE CONTACT THE LABORATORY BEFORE COLLECTING SPECIMENS FOR THIS TEST.  Samples must be analysed within 3-4 hours of collection.

Do not use 2 mL citrate tubes.

Transport to laboratory immediately. DO NOT transport in chute.

Non - BHH Collection: Must be transported at room temperature to the Box Hill Hospital laboratory within 2 hours.  Notify  laboratory before collection and record medication (including all painkillers.)

 PLEASE CONTACT THE LABORATORY BEFORE COLLECTING SPECIMENS FOR THIS TEST.  Samples must be analysed within 3-4 hours of collection.

Do not use 2 mL citrate tubes. Required 2x citrate tubes.

Transport to laboratory immediately. DO NOT transport in chute.

Non - BHH Collection: Must be transported at room temperature to the Box Hill Hospital laboratory within 2 hours.  Notify  laboratory before collection and record medication (including all painkillers.)

Processing Instructions

DO NOT centrifuge .

DO NOT centrifuge .

Notes

  • Used in screening for von Willebrand's disease and aspirin therapy.

Used in screening for von Willebrand's disease and aspirin therapy.


 Recent changes for Prothrombin Fragment 1 & 2

Recent changes for Prothrombin Fragment 1 & 2

Date
Field
Changed From
Changed To
23rd October 2018
External Laboratory

Austin Pathology

Monash Pathology


 Recent changes for Pseudocholinesterase

Recent changes for Pseudocholinesterase

Date
Field
Changed From
Changed To
7th February 2020
Assay name

Cholinesterase

Pseudocholinesterase

Collection & Request Instructions

Please request serum BCHE activity with the Genetic test.

Genetic testing sample : EDTA  whole blood 5mL minimum

 

 

 

Please request serum BCHE activity with the Genetic test.

Genetic testing sample : EDTA  whole blood 5mL minimum  and serum for BCHE activity.

 

 

 

30th January 2020
Alternative names

Pseudocholinesterase

Pseudocholinesterase, Butyrylcholinesterase

Preferred Container Type

Pink EDTA (6mL)

Pink EDTA (6mL)
Serum tube with gel

Collection & Request Instructions

Genetic testing sample : EDTA  whole blood 5mL minimum

 

Please request serum BCHE activity with the Genetic test.

Genetic testing sample : EDTA  whole blood 5mL minimum

 

 

 

Processing Instructions

Genetic testing on unspun whole blood is performed by:

Molecular Genetics Department, Pathwest

QE II Medical centre,

Hospital Ave, Nedlands WA.  6009

Phone Lab Results 08 6383 4223  Spec Recepn. 08 6383 4956

Enzyme testing (serum) : RPA (Sydney) Samples can be frozen, Batched & sent weekly

Genetic testing and enzyme testing: please send samples together packed with ice pack.

Please send patient financial consent form. Cost of the test: $150.

External Laboratory

Pathwest Lab Med WA

Sydney South West Pathology Service(RPA)

14th February 2019
Request Group

PCHOLS

6th August 2018
Processing Instructions

Genetic testing on unspun whole blood is performed by:

Molecular Genetics Department, Pathwest

QE II Medical centre,

Hospital Ave, Nedlands WA.  6009

Phone Lab Results 08 93462903  Spec Recepn. 08 6383 4956

Enzyme testing (serum) : RPA (Sydney) Samples can be frozen, Batched & sent weekly

Genetic testing on unspun whole blood is performed by:

Molecular Genetics Department, Pathwest

QE II Medical centre,

Hospital Ave, Nedlands WA.  6009

Phone Lab Results 08 6383 4223  Spec Recepn. 08 6383 4956

Enzyme testing (serum) : RPA (Sydney) Samples can be frozen, Batched & sent weekly

Frequency

Fortnightly


 Recent changes for Pyruvate

Recent changes for Pyruvate

Date
Field
Changed From
Changed To
2nd January 2019
Collection & Request Instructions

Pyruvate is very unstable and requires a special tube which is avalable from  the VCGS - Metabolic Laboratory Ph; 8341 6355. A fresh tube must be arranged for each request.

When the specimen is recieved the complete sample must be frozen immediately. Before sending the sample to VCGS, phone the laboatory to inform them it is coming.

 

Test not available.

For further information please contact chemical pathologist.

 

 

 

Processing Instructions

Write all the necessary collection details on the tube, do not add any other labels as the tube has been pre weighed. Place the pyruvate tube in a cup of ice water to cool for at least 5 mins before collection.

Draw 1 mL  ( 0.7 to 1.3 mL is acceptable ) of blood using a syringe and add this to the tube, mix well, the blood will turn chocolate brown.  TAKE CARE as the preservative in the tube is a corrosive acid. Ensure you are wearing protective gear and use safe practices. If the contents spill, wash the area well with water.

Place the specimen back into the ice water and transport immediately to the laboratory. Ensure a specimen for lactate is taken around the same time.

External Laboratory

Victorian Clinical Genetics Sevices (V.C.G.S) - Royal Childrens Hospital


 Recent changes for Quantiferon

Recent changes for Quantiferon

Date
Field
Changed From
Changed To
17th July 2019
Collection & Request Instructions

 PLEASE NOTE : Quantiferon specimens must now be collected using a dedicated Quantiferon Collection Pack containing four tubes (see image above)

Specimen must be tested within 10 hours of collection.

DO NOT refrigerate specimen.

Collect Monday - Friday before 10:00am.

Used to detect latent infection with M.tuberculosis, not for the diagnosis and management of active tuberculosis.

Note: Heparinised blood required, minimum 5 mL

 

 PLEASE NOTE : Quantiferon specimens must now be collected using a dedicated Quantiferon Collection Pack containing four tubes (see image above)

Specimen must be received at Box Hill by 16:00hrs Monday-Friday

DO NOT refrigerate specimen.

Used to detect latent infection with M.tuberculosis, not for the diagnosis and management of active tuberculosis.

Collection Centre

Latest Collection time

Latest courier time for transport

to BHH Microbiology

(must be received by 1600hrs)

 

Box Hill

1400 hrs

N/A

 

Maroondah

1400 hrs

1450 hrs

 

Angliss

1400 hrs

1415 hrs

 

Wantirna Health

1400 hrs

1515 hrs

 

Yarra Ranges

1100 hrs

1115 hrs

 

Peter James Centre

1100 hrs

 

1205 hrs

1020 hrs

Healesville/Yarra Valley

1200 hrs

1240 hrs

 

 

Processing Instructions

If the specimen is collected at Maroondah or Angliss Hospitals, please contact the BH Microbiology Dept (9895 3475) before sending the samples.

Do not centrifuge, refrigerate or freeze specimen.

If the specimen is collected outside of Box Hill Hospital, please contact the BH Microbiology Dept (9895 3475) before sending the samples.

Do not centrifuge, refrigerate or freeze specimen.

External Transport Instructions

When packing for transport, the specimens MUST be packed in a separate transport esky at room temperature.

DO NOT put in an esky with cold packs.

FOR MICROBIOLOGY STAFF

QUANTIFERON GOLD PROCESSING

Quantiferon Gold specimens must be processed as below before sending to VIDRL

Note: Tubes must be incubated within 16 hours of collection.

At 12.00-12.30 mix the Quantiferon Gold tubes by inverting 10 times.

Incubate the tubes UPRIGHT in incubator M120.

Write time of incubation in F8 notes.

The next day at 8 am centrifuge the tubes for 15 mins at 3000g

(Tubes must be incubated for 16 to 24 hours.)

Check tubes for complete separation, respin if necessary.

The tubes are then ready to be packed and sent to VIDRL

Write the incubation time (from F8 notes) on the cover sheet.

NOTE : There is no change to collection times. Store & transport specimens at room temperature.

When packing for transport, the specimens MUST be packed in a separate transport esky at room temperature.

DO NOT put in an esky with cold packs.

FOR MICROBIOLOGY STAFF

Quantiferon Gold specimens must be processed as below before sending to VIDRL

Note:

Tubes must be incubated within 16 hours of collection (keep at room temperature until incubation);

Incubation at 37°C must be for 16-24 hours;

After incubation, tubes must be centrifuged within 3 days (keep at room temperature until centrifugation).

QUANTIFERON GOLD PROCESSING

Processing: to be performed by Microbiology staff

Remix all tubes immediately before incubation by inverting 10 times

Incubate at 37°C, O2 (M138 incubator). There is a metal rack in the incubator to stand the tubes upright during incubation

For specimens received between 0800 hrs and 1600 hrs, incubate overnight.

For specimens received after 1600 hrs, hold at room temperature and incubate at 0800 hrs the next day (for 24 hours) and sent to VIDRL on the following day (i.e. two days later)

At 0800hr each day, remove specimens from incubator and centrifuge for 15 minutes at 3000 RCF (g). The laboratory assistant will do this on weekdays, Blood Culture Bench Scientist will do this on Saturday and Sunday

After centrifugation, send specimens to VIDRL with the 1230 hrs courier

Specimens that are received in the laboratory, greater than 10 hours after collection will not be tested (KIMMS).


 Recent changes for RAST

Recent changes for RAST

Date
Field
Changed From
Changed To
1st April 2019
Collection & Request Instructions

White top tube is the preferred sample.

0.2ml of blood required for each allergen.

Blood collection: still can be done after skin allergy testing.

White top tube is the preferred sample.0.2ml of blood required for each allergen.

Blood collection: still can be done after skin allergy testing.

Omega-5-Gliadin: serum send to Melbourne Pathology

IgG 96 Food Allergy test is not a RAST test  It is performed at  Australian Clinical Labs.This test is NOT rebatable and costs approx  $260.

Processing Instructions

IgG 96 Food Allergy test is not a RAST test  It is performed at  Australian Clinical Labs.This test is NOT rebatable and costs approx  $260.

 

Omega-5-Gliadin: send to Melborne Pathology

IgG 96 Food Allergy test is not a RAST test  It is performed at  Australian Clinical Labs.This test is NOT rebatable and costs approx  $260.

 

29th November 2018
Alternative names

Allergy IgE Antibodies, Allergy testing, Specific IgE Testing For Allergens

Allergy IgE Antibodies, Allergy testing, Specific IgE Testing For Allergens. SIgE

28th August 2018
Alternative names

Allergen Specific IgE

Specific IgE Testing For Allergens, Allergy IgE Antibodies, Allergy testing

Collection & Request Instructions

White top tube is the preferred sample.

0.2ml of blood required for each allergen.

White top tube is the preferred sample.

0.2ml of blood required for each allergen.

Blood collection: still can be done after skin allergy testing.

Processing Instructions

IgG 96 Food Allergy test is not a RAST test  It is performed at Pathlab ( Dorovitch Pathology - intergrative Medicine)  Phone No 88313000 the test is NOT rebatable and costs approx  $270.

Eastern Health Pathology is NOT to act as courier for this test the patient is to be referred to PathLab.

IgG 96 Food Allergy test is not a RAST test  It is performed at  Australian Clinical Labs.This test is NOT rebatable and costs approx  $260.

 

Frequency

Twice weekly

Notes

  • Specify each allergen required.

Specify each allergen required.


 Recent changes for Renin - plasma

Recent changes for Renin - plasma

Date
Field
Changed From
Changed To
3rd December 2019
Collection & Request Instructions

 

Collection Requirements for both or either tests

Collect into 4 mL EDTA tubes at Room Temperature. Do not collect on ice

If both Renin and Aldosterone are requested together, they can be collected into the same EDTA tube

In the laboratory, spin and separate the plasma immediately. Plasma is stable at Room Temperature if analysis is performed on the same day.

Renin must be frozen at -80 C. Transport plasma at room temperature until it can be place in -80 C Freezer.(Must be done within 3-4).

Add-on tests for Renin and Aldosterone should not be done on samples which have been previously collected or stored at 4 C.

Renin will not be collected at Healesville Hospital and Yarra Ranges Health Collection Rooms.

 

Collection Requirements for both or either tests

Collect into 4 mL EDTA tubes at Room Temperature. Do not collect on ice

If both Renin and Aldosterone are requested together, they can be collected into the same EDTA tube

In the laboratory, spin and separate the plasma immediately. Plasma is stable at Room Temperature if analysis is performed on the same day.

Renin must be frozen at -80 C. Transport plasma at room temperature (Maroondah and Angliss) until it can be place in -80 C Freezer.(Must be done within 3-4 hors).

Add-on tests for Renin and Aldosterone should not be done on samples which have been previously collected or stored at 4 C.

Renin will not be collected at Healesville Hospital and Yarra Ranges Health Collection Rooms.

3rd December 2019
Collection & Request Instructions

 

Collection Requirements for both or either tests

  • Collect into 4 mL EDTA tubes at Room Temperature. Do not collect on ice
  • If both Renin and Aldosterone are requested together, they can be collected into the same EDTA tube
  • In the laboratory, spin and separate the plasma immediately. Plasma is stable at Room Temperature if analysis is performed on the same day
  • If the specimen arrives on the weekend or after hours, or if the specimen is referred from an external laboratory, freeze the plasma
  • EDTA plasma samples from external laboratories should be stored and transported frozen
  • Whole blood or plasma stored at 4 Deg C is not suitable
  • Add-on tests for Renin and Aldosterone should not be done on samples which have been previously collected or stored at 4 Deg C

 

Collection Requirements for both or either tests

Collect into 4 mL EDTA tubes at Room Temperature. Do not collect on ice

If both Renin and Aldosterone are requested together, they can be collected into the same EDTA tube

In the laboratory, spin and separate the plasma immediately. Plasma is stable at Room Temperature if analysis is performed on the same day.

Renin must be frozen at -80 C. Transport plasma at room temperature until it can be place in -80 C Freezer.(Must be done within 3-4).

Add-on tests for Renin and Aldosterone should not be done on samples which have been previously collected or stored at 4 C.

Renin will not be collected at Healesville Hospital and Yarra Ranges Health Collection Rooms.

Processing Instructions

Spin,seperate and freeze, transport frozen.

Minimum plasma volume: 500 ul (1mL if aldosterone is also requested)

Spin,seperate and freeze (-80 C), transport frozen.

Minimum plasma volume: 500 ul (1mL if aldosterone is also requested)

Notes

Prior to testing, patients should be withdrawn from diuretics, ACE inhibitors, angiotensin II receptor antagonists, beta blockers and calcium channel blockers for 3 weeks. Patients should also be well hydrated, have had 7g/day of salt for one week and have normal plasma potassium and bicarbonate levels.

  •  Deliver to laboratory ASAP at room temperature

Prior to testing, patients should be withdrawn from diuretics, ACE inhibitors, angiotensin II receptor antagonists, beta blockers and calcium channel blockers for 3 weeks. Patients should also be well hydrated, have had 7g/day of salt for one week and have normal plasma potassium and bicarbonate levels.

 Deliver to laboratory ASAP at room temperature


 Recent changes for Respiratory Virus PCR

Recent changes for Respiratory Virus PCR

Date
Field
Changed From
Changed To
3rd July 2019
Notes

This PCR will detect and identify: Influenza A virus, Influenza B virus and Respiratory syncytial virus (RSV).

Transport at room temperature to the Laboratory

Testing is performed at Box Hill, Angliss, and Maroondah.

Testing  Lab

Testing hours

TAT (from receipt in lab)

All sites

 

Available 24/7 

1 hr

Samples received after hours may have a longer TAT due to other urgent critical pathology testing occuring.

This PCR will detect and identify: Influenza A virus, Influenza B virus and Respiratory syncytial virus (RSV).

Transport at room temperature to the Laboratory

Testing is performed at Box Hill, Angliss, and Maroondah.

Testing  Lab

Testing hours

TAT (from receipt in lab)

All sites

 

Available 24/7 

1 hr

Samples received after hours may have a longer TAT due to other urgent critical pathology testing occurring.

3rd July 2019
Notes

This PCR will detect and identify: Influenza A virus, Influenza B virus and Respiratory syncytial virus (RSV).

Transport at room temperature to the Laboratory

Testing is performed at Box Hill, Angliss, and Maroondah.

Testing  Lab

Testing hours

TAT (from receipt in lab)

 

All sites

 

Available 24/7 

1 hr

Samples received after hours may have a longer TAT due to other urgent critical pathology testing occuring.

This PCR will detect and identify: Influenza A virus, Influenza B virus and Respiratory syncytial virus (RSV).

Transport at room temperature to the Laboratory

Testing is performed at Box Hill, Angliss, and Maroondah.

Testing  Lab

Testing hours

TAT (from receipt in lab)

All sites

 

Available 24/7 

1 hr

Samples received after hours may have a longer TAT due to other urgent critical pathology testing occuring.

3rd July 2019
Notes

This PCR will detect and identify: Influenza A virus, Influenza B virus and Respiratory syncytial virus (RSV).

Transport at room temperature to the Laboratory

Testing is performed at Box Hill, Angliss, and Maroondah.

Testing  Lab

Testing hours

TAT (from receipt in lab)

 

All sites

 

Available 24/7 

1 hr

TAT is a guide only. Minimal staffing levels after hours may mean a longer TAT due to other urgent pathology testing occuring (ie urgent Troponin, urgent transfusions etc).

This PCR will detect and identify: Influenza A virus, Influenza B virus and Respiratory syncytial virus (RSV).

Transport at room temperature to the Laboratory

Testing is performed at Box Hill, Angliss, and Maroondah.

Testing  Lab

Testing hours

TAT (from receipt in lab)

 

All sites

 

Available 24/7 

1 hr

Samples received after hours may have a longer TAT due to other urgent critical pathology testing occuring.

3rd July 2019
Method

All tests for respiratory virus PCR are considered urgent and must be delivered to the laboratory ASAP. Overnight specimens collected at BHH must be delivered to Microbiology by 7 a.m. Monday-Sunday.

If ICU/Oncology patients tested  Flu A/B and RSV negative on GeneXpert will be referred to Monash for respiratory virus panel. This includes Influenza A virus, Influenza B virus, Respiratory Syncitial Virus, Picornavirus (RV/EV), Parainfluenza viruses 1,2 and 3, Adenovirus, Human Metapneumovirus, Bordetella pertussis, Bordetella parapertussis, Mycoplasma pneumoniae, Legionella species, Chlamydiaceae.

The following specimens will be referred for testing-

Sputum, Bronchial Washings, Bronchoalveolar Lavages

Specimens for Respiratory Multiplex PCR will be referred to Monash Molecular Laboratory

Contact Monash Pathology 9594 4532 or if no answer try Bacteriology 9594 4565 as there is someone rostered 24/7

 

All tests for respiratory virus PCR are considered urgent and must be delivered to the laboratory ASAP.

ICU/Oncology patients testing negative for Flu A/B and RSV  will be referred to Monash for respiratory virus panel. This includes Influenza A virus, Influenza B virus, Respiratory Syncitial Virus, Picornavirus (RV/EV), Parainfluenza viruses 1,2 and 3, Adenovirus, Human Metapneumovirus, Bordetella pertussis, Bordetella parapertussis, Mycoplasma pneumoniae, Legionella species, Chlamydiaceae.

The following specimens will be referred for testing-

Sputum, Bronchial Washings, Bronchoalveolar Lavages

Specimens for Respiratory Multiplex PCR will be referred to Monash Molecular Laboratory

Contact Monash Pathology 9594 4532 or if no answer try Bacteriology 9594 4565 as there is someone rostered 24/7

 

Notes

This PCR will detect and identify: Influenza A virus, Influenza B virus and Respiratory syncytial virus (RSV).

Transport at room temperature to the Laboratory

Testing is performed at BHH and MH.

Testing  Lab

Testing hours

TAT (from receipt in lab)

BHH

 – all sites except MH

Mon-Fri 0700-2000 hrs

Sat & Sun 0700-1630 hrs

1 hr

MH

1 June – 31 August 2018 24/7

Other times as for BHH

I hr

 

This PCR will detect and identify: Influenza A virus, Influenza B virus and Respiratory syncytial virus (RSV).

Transport at room temperature to the Laboratory

Testing is performed at Box Hill, Angliss, and Maroondah.

Testing  Lab

Testing hours

TAT (from receipt in lab)

 

All sites

 

Available 24/7 

1 hr

TAT is a guide only. Minimal staffing levels after hours may mean a longer TAT due to other urgent pathology testing occuring (ie urgent Troponin, urgent transfusions etc).


 Recent changes for Sexually Transmitted Infection Microscopy & Culture

Recent changes for Sexually Transmitted Infection Microscopy & Culture

Date
Field
Changed From
Changed To
3rd August 2018
Preferred Container Type

Genital swab kit

Genital swab kit + sterile pot

3rd August 2018
Preferred Container Type

Genital swab kit + sterile pot

Genital swab kit

3rd August 2018
Preferred Specimen Type

Genital swab

Genital swab
First stream urine

1st August 2018
Collection & Request Instructions

http://pathology.easternhealth.org.au/handbook/downloads/microbiology/Microbiology_Specimen_Collection_Guide.doc

 

Microbiology Collection Guide

http://pathology.easternhealth.org.au/handbook/downloads/microbiology/Microbiology_Specimen_Collection_Guide.doc

 

31st July 2018
Preferred Container Type

Microbiology collection kit

Genital swab kit + sterile pot


 Recent changes for Swabs for microscopy and culture

Recent changes for Swabs for microscopy and culture

Date
Field
Changed From
Changed To
1st August 2018
Preferred Container Type

Wound swab kit

Swab - blue top (Amies transport media)

Collection & Request Instructions

Specimen Collection

•Label the transport swab with the patient’s full name, date of birth, UR number  (if applicable), date, time and site of collection.

•Label the slide and slide carrier with the patient’s full name, and UR number (if applicable). For open wounds, clean with a swab moistened with normal saline until red granulation is visible.

•For closed wounds clean away excess debris and purulent material from the opening.

 

Use for wounds, exudates, eyes, ears, throat, skin and mucosal surfaces.

Transport at room temperature to the Laboratory

 

Specimen Collection

•Label the transport swab with the patient’s full name, date of birth,

•For closed wounds clean away excess debris and purulent material from the opening.

Microbiology Specimen Collection Guide

Use for wounds, exudates, eyes, ears, throat, skin and mucosal surfaces.

Transport at room temperature to the Laboratory

 

Notes

Wound  Swab Collection Instructions 

Wound cultures Clinical Information

Throat Swab Collection Instructions

Throat cultures Clinical Information

Nasal Swab Collection Instructions

The transit of specimen from Angliss or Maroondah lab and out of working hour collection may cause the prolonged turnaround time

Microbiology Specimen Collection Guide

Wound cultures Clinical Information

Throat Swab Collection Instructions

Throat cultures Clinical Information

Nasal Swab Collection Instructions

The transit of specimen from Angliss or Maroondah lab and out of working hour collection may cause the prolonged turnaround time


 Recent changes for Testosterone

Recent changes for Testosterone

Date
Field
Changed From
Changed To
15th February 2019
Processing Instructions

Free Testosterone/Calculated free testosterone requested: test code CFTEST

Also add Testosterone/SHBG/Albumin if free testosterone requested.


 Recent changes for Thiopurine Metabolites

Recent changes for Thiopurine Metabolites

Date
Field
Changed From
Changed To
3rd September 2019
Collection & Request Instructions

Collect 2x4ml EDTA blood. DO NOT SPIN

Keep at 4 deg C, do not freeze.

The metabolites are stable for upto 4 days at 4 degress.

Collect 2x4ml EDTA blood. DO NOT SPIN

Keep at 4 deg C, do not freeze.

The metabolites are stable for upto 4 days at 4 degress.

Notes

Imuran is a trade name for azathioprine

The Thiopurine metabolites (6-TGN & 6-MMP) are used as a means of estimating the toxicity and efficacy of azathioprine treatment.

This test is performed by the Gastroenerology department. ( Phone No.9895 4632 )

The cost for the test is not covered by Medicare. Contact the testing lab for cost details.

Imuran is a trade name for azathioprine

The Thiopurine metabolites (6-TGN & 6-MMP) are used as a means of estimating the toxicity and efficacy of azathioprine treatment.

This test is performed by the Gastroenerology department. ( Phone No.9895 4632 )

The cost for the test is not covered by Medicare

Eastern Health patients:6TGN 6MMP Thiopurine Metabolites Testing $75

All other patients: 6TGN 6MMP Thiopurine Metabolites Testing $145


 Recent changes for Trichomonas PCR

Recent changes for Trichomonas PCR

Date
Field
Changed From
Changed To
10th January 2019
Collection & Request Instructions

Transport specimen at Room Temperature to lab

10th January 2019
Assay name

Trichomonas

Trichomonas PCR

Alternative names

Trich, Trichomonas

Request Group

GMC

OPCR

Preferred Specimen Type

Swab

Vaginal swab
Endocervical swab
Urine

Preferred Container Type

Wound swab kit

Genital swab kit + sterile pot
Genital swab kit

Collection & Request Instructions

Urine

15 - 20 mL of first-stream urine specimen collected at least one hour since the patient last voided. Refrigerate immediately

Cervical  & Vaginal swabs

Use plastic swab in plain tube without transport medium (wire swab not suitable)

ALL Specimens must reach the Pathology laboratory within 2 hours of collection.

http://pathology.easternhealth.org.au/handbook/downloads/microbiology/Microbiology_Specimen_Collection_Guide.doc

Frequency

Daily

Twice weekly

Notes

http://pathology.easternhealth.org.au/handbook/downloads/microbiology/Microbiology_Specimen_Collection_Guide.doc


 Recent changes for Urine Nickel

Recent changes for Urine Nickel

Date
Field
Changed From
Changed To
17th January 2020
External Laboratory

Sydney South West Pathology Service(RPA)

Alfred Hospital

Frequency

As required


 Recent changes for VRE Screen

Recent changes for VRE Screen

Date
Field
Changed From
Changed To
1st August 2018
Collection & Request Instructions

A rectal swab is required.

 

A rectal swab is required.

Microbiology Specimen Collection Guide.

http://pathology.easternhealth.org.au/handbook/downloads/microbiology/Microbiology_Specimen_Collection_Guide.doc

31st July 2018
Collection & Request Instructions

A rectal swab is required.

Not in transport medium.

A rectal swab is required.

 

31st July 2018
Alternative names

VRE

Preferred Container Type

Swab - red top, plain swab, wooden shaft

Swab - blue top (Amies transport media)