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Recent Changes - Test & Collection Guide

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 Recent changes for Adrenocorticotrophic Hormone

Recent changes for Adrenocorticotrophic Hormone

Date
Field
Changed From
Changed To
2nd August 2022
Notes

Consultation with Endocrinologist suggested

Collect in the morning between 0800 and 1000h.

1 x 4mL EDTA tube on ice.

Send to laboratory for immediate centrifugation. Spin, separate and freeze plasma. Transport frozen.

 

 

 

Consultation with Endocrinologist suggested

Collect 1 x 4mL EDTA tube on ice.

Send to laboratory for immediate centrifugation. Spin, separate and freeze plasma. 

 

 

 


 Recent changes for Alpha-1 Antitrypsin - Quantitation

Recent changes for Alpha-1 Antitrypsin - Quantitation

Date
Field
Changed From
Changed To
13th September 2022
External Laboratory

St Vincents Hospital


 Recent changes for Anti-diuretic hormone

Recent changes for Anti-diuretic hormone

Date
Field
Changed From
Changed To
7th October 2021
Alternative names

ADH

Request Group

ADH

Preferred Specimen Type

Plasma

Serum

Optional Specimen Type

Plasma

Preferred Container Type

EDTA

Serum tube with gel

Optional Container Type

EDTA
Lithium heparin no gel

Collection & Request Instructions

Samples should be fasting.

Processing Instructions

Spin, separate and freeze plasma.

Packed by certified personnel.

Sent frozen.

Specimen Reception, Level 6 (E Block), RPA, Missenden Road, Camperdown , NSW 2050

Tel: 02-95158279

Spin, separate and freeze serum/plasma

Packed by certified personnel.

Sent frozen.

Specimen Reception, Level 6 (E Block), RPA, Missenden Road, Camperdown , NSW 2050

Tel: 02-95158279


 Recent changes for Anti-Xa Assay

Recent changes for Anti-Xa Assay

Date
Field
Changed From
Changed To
18th May 2021
Request Group

10A: Clexane, 10AH:Plasma Heparin, APIX: Apixaban, RIVX: Rivaroxaban

10A: Clexane, 10AH:Plasma Heparin, APIX: Apixaban, RIVX: Rivaroxaban, FONX: Fondaparinux

18th May 2021
Request Group

10A

10A: Clexane, 10AH:Plasma Heparin, APIX: Apixaban, RIVX: Rivaroxaban


 Recent changes for Azathioprine Metabolite Testing

Recent changes for Azathioprine Metabolite Testing

Date
Field
Changed From
Changed To
23rd August 2022
Processing Instructions

Place the samples and request slip in fridge (M43) in CSR. Do not enter in the computer.

Eastern Health Clinical School
Level 2, 5 Arnold Street,
Box Hill  Vic  3128
Ph: 61 3 9091 8827
Fax: 61 3 9899 9137
E-mail:  ehcs@monash.edu


 Recent changes for Bile Acids

Recent changes for Bile Acids

Date
Field
Changed From
Changed To
23rd May 2022
Laboratory

Referred Test

Biochemistry

Preferred Container Type

Plain Tube with gel

Serum tube with gel

Collection & Request Instructions

Fasting or Non Fasting sample.

Note: This test is ONLY performed on pregnant women.

 

Fasting.

Note: This test is ONLY performed on pregnant women.

 

Processing Instructions

Spin, separate ASAP.

External Laboratory

Royal Childrens Hospital

Frequency

Weekly

Twice weekly

Method

Store and transport at 4 C.


 Recent changes for Blood Cultures - Adult

Recent changes for Blood Cultures - Adult

Date
Field
Changed From
Changed To
9th August 2021
Processing Instructions

Bottles should be loaded on to the Bactec analyser as soon as possible. If entry is delayed, store bottles at room temperature.

Bottles should be loaded on to the Bactec FX analyser as soon as possible. If entry is delayed, store bottles at room temperature.

9th August 2021
Processing Instructions

Bottles should be loaded on to the Virtuo analyser as soon as possible. If entry is delayed, store bottles at room temperature.

Bottles should be loaded on to the Bactec analyser as soon as possible. If entry is delayed, store bottles at room temperature.

25th May 2021
Volume (Adults)

10 mL

8 mL

Preferred Container Type

Blood Culture

Blood Culture (Adult)


 Recent changes for Blood Cultures - Children and Neonates

Recent changes for Blood Cultures - Children and Neonates

Date
Field
Changed From
Changed To
9th August 2021
External Transport Instructions

Load as soon as possible onto the Virtuo Analyzer. Store at room temperature until loaded

Load as soon as possible onto the Bactec FX Analyzer. Store at room temperature until loaded

25th May 2021
Volume (Adults)

4 mL

Minimum/Paediatric Volume

1 mL

Preferred Container Type

Blood Culture Paediatric

Blood Culture (Paediatric)

Collection & Request Instructions

Add 0.5 - 4mL of blood to a single aerobic paediatric bottle.

For patients with suspected bacterial endocarditis, 2 to 3 sets taken at different times should be sufficient to establish a diagnosis.

Collect blood cultures 20 minutes apart. If patient is too ill to wait 20 minutes, prior to administering antibiotics, collect the first set from one site and the second from a different site.

Please provide clinical details. Extended incubation is required for query endocarditis, brucellosis or fungal infection

Transport at room temperature

 

Add 1 - 3mL of blood to a single aerobic paediatric bottle.

For patients with suspected bacterial endocarditis, 2 to 3 sets taken at different times should be sufficient to establish a diagnosis.

If multiple sets required, collect blood cultures 20 minutes apart. If patient is too ill to wait 20 minutes, prior to administering antibiotics, collect the first set from one site and the second from a different site.

Please provide clinical details. Extended incubation is required for query endocarditis, brucellosis or fungal infection

Transport at room temperature

 


 Recent changes for Bone Marrow Examination

Recent changes for Bone Marrow Examination

Date
Field
Changed From
Changed To
25th March 2022
Laboratory

Unassigned

Haematology


 Recent changes for Bone Specific Alkaline Phosphatase

Recent changes for Bone Specific Alkaline Phosphatase

Date
Field
Changed From
Changed To
15th June 2021
Assay name

Bone Specific Alkiline Phosphatase

Bone Specific Alkaline Phosphatase

External Laboratory

Monash Pathology

Royal Melbourne Hospital


 Recent changes for BRCA1/BRCA2

Recent changes for BRCA1/BRCA2

Date
Field
Changed From
Changed To
17th June 2021
Request Group

CHROM

Collection & Request Instructions

Send to Peter Mac on next available courier. Stable at room temperature for a maximum of 2 weeks.

Processing Instructions

Send to Peter Mac on next available courier. Stable at room temperature for a maximum of 2 weeks.

External Transport Instructions

Room Temperature

Room Temperature

Send to Peter Mac on next available courier. Stable at room temperature for a maximum of 2 weeks.

Method

Send to Peter Mac on next available courier. Stable at room temperature for a maximum of 2 weeks.


 Recent changes for Cadmium

Recent changes for Cadmium

Date
Field
Changed From
Changed To
13th September 2022
Notes

Transport specimen at Room Temperature to lab
Sample haemolysis may make this test unreportable

 

 

Transport specimen at Room Temperature to lab
Sample haemolysis may make this test unreportable

Send as whole blood

 

 


 Recent changes for Caeruloplasmin

Recent changes for Caeruloplasmin

Date
Field
Changed From
Changed To
13th September 2022
External Laboratory

Austin Pathology


 Recent changes for CASA

Recent changes for CASA

Date
Field
Changed From
Changed To
17th February 2022
External Laboratory

Royal Brisbane & Womens Hospital

External Transport Instructions

  • Transport frozen.
  • Packed by certified personnel.
  • Transport frozen.

    Packed by certified personnel.

    Notes

    In combination with the tumour marker CA-125, CASA is used for differentiating between benign gynaecological disease and ovarian carcinoma.  Please note that this test has changed referral laboratories as at 18/1/2011.

    Please contact Chemical Pathologist before requesting this test.

    In combination with the tumour marker CA-125, CASA is used for differentiating between benign gynaecological disease and ovarian carcinoma.  Please note that this test has changed referral laboratories as at 18/1/2011.  This test is no longer available in Australia

    Please contact Chemical Pathologist before requesting this test.

     


     Recent changes for Cerebrospinal Fluid - Biochemistry

    Recent changes for Cerebrospinal Fluid - Biochemistry

    Date
    Field
    Changed From
    Changed To
    27th April 2021
    Collection & Request Instructions

    Please note : to avoid sample deterioration, DO NOT send CSF specimens via the vacuum chute system.

    Depending on the tests requested, 3-4 separate tubes will need to be provided.  Note that the tubes are numbered so that order of collection can be identified – this order should be carefully followed, so that the optimal sample can be used for the requested tests.  It is also important to consider the tests that are required, as this will determine how much CSF is needed in each tube.  See the matrix below for details.

    TUBE 1 is used for Cytology.

    TUBE 2 is used for Glucose, Protein and Flow Cytometry.

    (centrifuged in the lab – the supernatant is used for Biochemistry analysis (glucose, protein) and the cellular pellet will be used for flow cytometry if required).

    TUBE 3 is for Microbiological analysis (cell count, Gram stain and culture) and any further infectious testing (e.g. viral PCR, mycobacterial PCR and culture, cryptococcal antigen).

    TUBE 4 will be required only for;

             (a) Creutzfeld-Jacob Disease (CJD) testing OR

    (b) Xanthochromia analysis (wrap the tube in foil to prevent    sample deterioration due to light).

     

    TESTS REQUIRED

    MINIMUM REQUIRED VOLUME (mL)

    TUBE 1

    TUBE 2

    TUBE 3

    TUBE 4

    CYTOLOGY. CHEMISTRY, MICRO/CULTURE

    1.0-2.0

    1.0

    1.0-2.0

    Nil

    ADD FLOW CYTOMETRY

     

    add 5.0-7.0

     

     

    ADD OTHER INFECTIOUS MARKERS

     

     

    add 2.0- 5.0-

     

    ADD MYCOBACTERIAL CULTURE

     

     

    Add 10.00

     

    ADD CJD

     

     

     

    1.0

    ADD XANTHOCHROMIA

     

     

     

    1.0 (foil)

     

    Note, the ability to add further tests to a previously collected sample is dependent upon the volume provided.  Also, many tests (flow cytometry, cytology) can only be performed on fresh specimens.

     

    Other specialised testing (e.g. oligoclonal bands) will be performed using the supplied samples.

     

    Xanthochromia and CJD analysis cannot be performed on a blood-stained sample.

    CSF Information - Laboratory Processing

    Depending on the tests requested, 3-4 separate tubes will need to be provided. Note that the tubes are numbered so that order of collection can be identified – this order should be carefully followed, so that the optimal sample can be used for the requested tests. It is also important to consider the tests that are required, as this will determine how much CSF is needed in each tube. See the matrix below for details.

    TUBE 1 is used for Biochemistry and Flow Cytometry. (Centrifuged in the lab – the supernatant is used for Biochemistry analysis (glucose, protein) and the cellular pellet will be used for flow cytometry if required). If tube 3 is bloodstained, take an aliquot for microbiology.

    TUBE 2 is used for Cytology

    TUBE 3 is for Microbiological analysis (cell count, Gram stain and culture) and any further infectious testing (e.g. viral PCR, mycobacterial PCR and culture, cryptococcal antigen).

    TUBE 4 will be required only for;

    (a) Creutzfeld-Jacob Disease (CJD) testing OR

    (b) Xanthochromia analysis (wrap the tube in foil to prevent sample deterioration due to light).

     

    TESTS REQUIRED

    MINIMUM REQUIRED VOLUME (mL)

    TUBE 1

    TUBE 2

    TUBE 3

    TUBE 4

    CHEMISTRY

    1.0

         

    ADD FLOW CYTOMETRY

    Minimum 5.0-7.0

         

    CYTOLOGY

     

    1.0-3.0

       

    MICROBIOLOGY

       

    1.0-2.0

     

    ADD OTHER INFECTIOUS MARKERS

       

    add 2.0- 5.0-

     

    ADD MYCOBACTERIAL CULTURE

       

    Add 6.0-10.00

     

    ADD CJD

         

    1.0

    ADD XANTHOCHROMIA

         

    1.0 (foil)

     

    Note, the ability to add further tests to a previously collected sample is dependent upon the volume provided. Also, many tests (flow cytometry, cytology) can only be performed on fresh specimens.

     

    Other specialised testing (e.g. oligoclonal bands) will be performed using the supplied samples.

    Xanthochromia and CJD analysis cannot be performed on a blood-stained sample.

     


     Recent changes for Cerebrospinal Fluid - Cytology

    Recent changes for Cerebrospinal Fluid - Cytology

    Date
    Field
    Changed From
    Changed To
    27th April 2021
    Collection & Request Instructions

    The sample with the least amount of blood is prefered for Cytology.

    CSF SPECIMEN INFORMATION – LABORATORY PROCESSING

    Depending on the tests requested, 3-4 separate tubes will need to be provided.  Note that the tubes are numbered so that order of collection can be identified – this order should be carefully followed, so that the optimal sample can be used for the requested tests.  It is also important to consider the tests that are required, as this will determine how much CSF is needed in each tube.  See the matrix below for details.

    TUBE 1 is used for Cytology.

    TUBE 2 is used for Glucose, Protein and Flow Cytometry.

    (centrifuged in the lab – the supernatant is used for Biochemistry analysis (glucose, protein) and the cellular pellet will be used for flow cytometry if required).

    TUBE 3 is for Microbiological analysis (cell count, Gram stain and culture) and any further infectious testing (e.g. viral PCR, mycobacterial PCR and culture, cryptococcal antigen).

    TUBE 4 will be required only for;

             (a) Creutzfeld-Jacob Disease (CJD) testing OR

    (b) Xanthochromia analysis (wrap the tube in foil to prevent    sample deterioration due to light).

     

    TESTS REQUIRED

    MINIMUM REQUIRED VOLUME (mL)

    TUBE 1

    TUBE 2

    TUBE 3

    TUBE 4

    CYTOLOGY. CHEMISTRY, MICRO/CULTURE

    1.0-2.0

    1.0

    1.0-2.0

    Nil

    ADD FLOW CYTOMETRY

     

    add 5.0-7.0

     

     

    ADD OTHER INFECTIOUS MARKERS

     

     

    add 2.0- 5.0-

     

    ADD MYCOBACTERIAL CULTURE

     

     

    Add 10.00

     

    ADD CJD

     

     

     

    1.0

    ADD XANTHOCHROMIA

     

     

     

    1.0 (foil)

     

    Note, the ability to add further tests to a previously collected sample is dependent upon the volume provided.  Also, many tests (flow cytometry, cytology) can only be performed on fresh specimens.

     

    Other specialised testing (e.g. oligoclonal bands) will be performed using the supplied samples.

     

    Xanthochromia and CJD analysis cannot be performed on a blood-stained sample.

    The sample with the least amount of blood is prefered for Cytology.

    CSF SPECIMEN INFORMATION – LABORATORY PROCESSING

    Depending on the tests requested, 3-4 separate tubes will need to be provided.  Note that the tubes are numbered so that order of collection can be identified – this order should be carefully followed, so that the optimal sample can be used for the requested tests.  It is also important to consider the tests that are required, as this will determine how much CSF is needed in each tube.  See the matrix below for details.

    TUBE 1 is used for Biochemistry and Flow Cytometry. (Centrifuged in the lab – the supernatant is used for Biochemistry analysis (glucose, protein) and the cellular pellet will be used for flow cytometry if required). If tube 3 is bloodstained, take an aliquot for microbiology.

    TUBE 2 is used for Cytology

    TUBE 3 is for Microbiological analysis (cell count, Gram stain and culture) and any further infectious testing (e.g. viral PCR, mycobacterial PCR and culture, cryptococcal antigen).

    TUBE 4 will be required only for;

    (a) Creutzfeld-Jacob Disease (CJD) testing OR

    (b) Xanthochromia analysis (wrap the tube in foil to prevent sample deterioration due to light).

     

    TESTS REQUIRED

    MINIMUM REQUIRED VOLUME (mL)

    TUBE 1

    TUBE 2

    TUBE 3

    TUBE 4

    CHEMISTRY

    1.0

         

    ADD FLOW CYTOMETRY

    Minimum 5.0-7.0

         

    CYTOLOGY

     

    1.0-3.0

       

    MICROBIOLOGY

       

    1.0-2.0

     

    ADD OTHER INFECTIOUS MARKERS

       

    add 2.0- 5.0-

     

    ADD MYCOBACTERIAL CULTURE

       

    Add 6.0-10.00

     

    ADD CJD

         

    1.0

    ADD XANTHOCHROMIA

         

    1.0 (foil)

     

    Note, the ability to add further tests to a previously collected sample is dependent upon the volume provided. Also, many tests (flow cytometry, cytology) can only be performed on fresh specimens.

     

    Other specialised testing (e.g. oligoclonal bands) will be performed using the supplied samples.

    Xanthochromia and CJD analysis cannot be performed on a blood-stained sample.

     

     

    Method

    CSF sample processing:

    If Cytology/Flow Cytometry requested  : Tube 1-Biochemistry, Tube 2- Cytology/Flow Cytometry, Tube 3-Microbiology

    If Cytology/Flow Cytometry not requested: Tube 1 and 3-Microbiology, Tube 2-Biochemistry

    Any confusion between the terms cell count and cytology could be cleared up when the cell count is rung through to the requesting doctor by the scientist. At this point it could be determined whether cytology is a genuine request or not (all to be documented in F8 notes).

     

     


     Recent changes for Cerebrospinal Fluid - microscopy and culture

    Recent changes for Cerebrospinal Fluid - microscopy and culture

    Date
    Field
    Changed From
    Changed To
    27th April 2021
    Notes

    CSF SPECIMEN INFORMATION – LABORATORY PROCESSING

    Depending on the tests requested, 3-4 separate tubes will need to be provided.  Note that the tubes are numbered so that order of collection can be identified – this order should be carefully followed, so that the optimal sample can be used for the requested tests.  It is also important to consider the tests that are required, as this will determine how much CSF is needed in each tube.  See the matrix below for details.

    TUBE 1 is used for Cytology.

    TUBE 2 is used for Glucose, Protein and Flow Cytometry.

    (centrifuged in the lab – the supernatant is used for Biochemistry analysis (glucose, protein) and the cellular pellet will be used for flow cytometry if required).

    TUBE 3 is for Microbiological analysis (cell count, Gram stain and culture) and any further infectious testing (e.g. viral PCR, mycobacterial PCR and culture, cryptococcal antigen).

    TUBE 4 will be required only for;

             (a) Creutzfeld-Jacob Disease (CJD) testing OR

    (b) Xanthochromia analysis (wrap the tube in foil to prevent    sample deterioration due to light).

     

    TESTS REQUIRED

    MINIMUM REQUIRED VOLUME (mL)

    TUBE 1

    TUBE 2

    TUBE 3

    TUBE 4

    CYTOLOGY. CHEMISTRY, MICRO/CULTURE

    1.0-2.0

    1.0

    1.0-2.0

    Nil

    ADD FLOW CYTOMETRY

     

    add 5.0-7.0

     

     

    ADD OTHER INFECTIOUS MARKERS

     

     

    add 2.0- 5.0-

     

    ADD MYCOBACTERIAL CULTURE

     

     

    Add 10.00

     

    ADD CJD

     

     

     

    1.0

    ADD XANTHOCHROMIA

     

     

     

    1.0 (foil)

     

    Note, the ability to add further tests to a previously collected sample is dependent upon the volume provided.  Also, many tests (flow cytometry, cytology) can only be performed on fresh specimens.

     

    Other specialised testing (e.g. oligoclonal bands) will be performed using the supplied samples.

     

    Xanthochromia and CJD analysis cannot be performed on a blood-stained sample.

    Special tests available on request:

    PCR for viruses

    Acid-fast stain and/or culture for Mycobacteria

    Cryptococcal antigen

    Serological test for Syphilis (must be paired with 5mL serum sample or test will not be performed)

    Viral Serology, if CSF is accompanied by serum sample

    Flow Cytometry - requires a 4th tube

    The turnaround time for CSF microscopy are 30-40 minutes.

    The turnaround time for CSF culture are 3-5 days.

    Depending on the tests requested, 3-4 separate tubes will need to be provided. Note that the tubes are numbered so that order of collection can be identified – this order should be carefully followed, so that the optimal sample can be used for the requested tests. It is also important to consider the tests that are required, as this will determine how much CSF is needed in each tube. See the matrix below for details.

    TUBE 1 is used for Biochemistry and Flow Cytometry. (Centrifuged in the lab – the supernatant is used for Biochemistry analysis (glucose, protein) and the cellular pellet will be used for flow cytometry if required). If tube 3 is bloodstained, take an aliquot for microbiology.

    TUBE 2 is used for Cytology

    TUBE 3 is for Microbiological analysis (cell count, Gram stain and culture) and any further infectious testing (e.g. viral PCR, mycobacterial PCR and culture, cryptococcal antigen).

    TUBE 4 will be required only for;

    (a) Creutzfeld-Jacob Disease (CJD) testing OR

    (b) Xanthochromia analysis (wrap the tube in foil to prevent sample deterioration due to light).

     

    TESTS REQUIRED

    MINIMUM REQUIRED VOLUME (mL)

    TUBE 1

    TUBE 2

    TUBE 3

    TUBE 4

    CHEMISTRY

    1.0

         

    ADD FLOW CYTOMETRY

    Minimum 5.0-7.0

         

    CYTOLOGY

     

    1.0-3.0

       

    MICROBIOLOGY

       

    1.0-2.0

     

    ADD OTHER INFECTIOUS MARKERS

       

    add 2.0- 5.0-

     

    ADD MYCOBACTERIAL CULTURE

       

    Add 6.0-10.00

     

    ADD CJD

         

    1.0

    ADD XANTHOCHROMIA

         

    1.0 (foil)

     

    Note, the ability to add further tests to a previously collected sample is dependent upon the volume provided. Also, many tests (flow cytometry, cytology) can only be performed on fresh specimens.

     

    Other specialised testing (e.g. oligoclonal bands) will be performed using the supplied samples.

    Xanthochromia and CJD analysis cannot be performed on a blood-stained sample.


     Recent changes for Cerebrospinal Fluid Specimen Collection

    Recent changes for Cerebrospinal Fluid Specimen Collection

    Date
    Field
    Changed From
    Changed To
    27th April 2021
    Notes

    Depending on the tests requested, 3-4 separate tubes will need to be provided. Note that the tubes are numbered so that order of collection can be identified – this order should be carefully followed, so that the optimal sample can be used for the requested tests. It is also important to consider the tests that are required, as this will determine how much CSF is needed in each tube. See the matrix below for details.

    TUBE 1 is used for Biochemistry and Flow Cytometry. (Centrifuged in the lab – the supernatant is used for Biochemistry analysis (glucose, protein) and the cellular pellet will be used for flow cytometry if required). If tube 3 is bloodstained, take an aliquot for microbiology.

    TUBE 2 is used for Cytology

    TUBE 3 is for Microbiological analysis (cell count, Gram stain and culture) and any further infectious testing (e.g. viral PCR, mycobacterial PCR and culture, cryptococcal antigen).

    TUBE 4 will be required only for;

    (a) Creutzfeld-Jacob Disease (CJD) testing OR

    (b) Xanthochromia analysis (wrap the tube in foil to prevent sample deterioration due to light).

     

    TESTS REQUIRED

    MINIMUM REQUIRED VOLUME (mL)

    TUBE 1

    TUBE 2

    TUBE 3

    TUBE 4

    CHEMISTRY

    1.0

         

    ADD FLOW CYTOMETRY

    Minimum 5.0-7.0

         

    CYTOLOGY

     

    1.0-3.0

       

    MICROBIOLOGY

       

    1.0-2.0

     

    ADD OTHER INFECTIOUS MARKERS

       

    add 2.0- 5.0-

     

    ADD MYCOBACTERIAL CULTURE

       

    Add 6.0-10.00

     

    ADD CJD

         

    1.0

    ADD XANTHOCHROMIA

         

    1.0 (foil)

     

    Note, the ability to add further tests to a previously collected sample is dependent upon the volume provided. Also, many tests (flow cytometry, cytology) can only be performed on fresh specimens.

     

    Other specialised testing (e.g. oligoclonal bands) will be performed using the supplied samples.

    Xanthochromia and CJD analysis cannot be performed on a blood-stained sample.

     

     

    Depending on the tests requested, 3-4 separate tubes will need to be provided. Note that the tubes are numbered so that order of collection can be identified – this order should be carefully followed, so that the optimal sample can be used for the requested tests. It is also important to consider the tests that are required, as this will determine how much CSF is needed in each tube. See the matrix below for details.

    TUBE 1 is used for Biochemistry and Flow Cytometry. (Centrifuged in the lab – the supernatant is used for Biochemistry analysis (glucose, protein) and the cellular pellet will be used for flow cytometry if required). If tube 3 is bloodstained, take an aliquot for microbiology.

    TUBE 2 is used for Cytology

    TUBE 3 is for Microbiological analysis (cell count, Gram stain and culture) and any further infectious testing (e.g. viral PCR, mycobacterial PCR and culture, cryptococcal antigen).

    TUBE 4 will be required only for;

    (a) Creutzfeld-Jacob Disease (CJD) testing OR

    (b) Xanthochromia analysis (wrap the tube in foil to prevent sample deterioration due to light).

     

    TESTS REQUIRED

    MINIMUM REQUIRED VOLUME (mL)

    TUBE 1

    TUBE 2

    TUBE 3

    TUBE 4

    CHEMISTRY

    1.0

         

    ADD FLOW CYTOMETRY

    Minimum 5.0-7.0

         

    CYTOLOGY

     

    1.0-3.0

       

    MICROBIOLOGY

       

    1.0-2.0

     

    ADD OTHER INFECTIOUS MARKERS

       

    add 2.0- 5.0-

     

    ADD MYCOBACTERIAL CULTURE

       

    Add 6.0-10.00

     

    ADD CJD

         

    1.0

    ADD XANTHOCHROMIA

         

    1.0 (foil)

     

    Note, the ability to add further tests to a previously collected sample is dependent upon the volume provided. Also, many tests (flow cytometry, cytology) can only be performed on fresh specimens.

     

    Other specialised testing (e.g. oligoclonal bands) will be performed using the supplied samples.

    Xanthochromia and CJD analysis cannot be performed on a blood-stained sample.

     

    27th April 2021
    Notes

    CSF SPECIMEN INFORMATION – LABORATORY PROCESSING

    Depending on the tests requested, 3-4 separate tubes will need to be provided.  Note that the tubes are numbered so that order of collection can be identified – this order should be carefully followed, so that the optimal sample can be used for the requested tests.  It is also important to consider the tests that are required, as this will determine how much CSF is needed in each tube.  See the matrix below for details.

    TUBE 1 is used for Cytology.

    TUBE 2 is used for Glucose, Protein and Flow Cytometry.

    (centrifuged in the lab – the supernatant is used for Biochemistry analysis (glucose, protein) and the cellular pellet will be used for flow cytometry if required).

    TUBE 3 is for Microbiological analysis (cell count, Gram stain and culture) and any further infectious testing (e.g. viral PCR, mycobacterial PCR and culture, cryptococcal antigen).

    TUBE 4 will be required only for;

             (a) Creutzfeld-Jacob Disease (CJD) testing OR

    (b) Xanthochromia analysis (wrap the tube in foil to prevent    sample deterioration due to light).

     

    TESTS REQUIRED

    MINIMUM REQUIRED VOLUME (mL)

    TUBE 1

    TUBE 2

    TUBE 3

    TUBE 4

    CYTOLOGY. CHEMISTRY, MICRO/CULTURE

    1.0-2.0

    1.0

    1.0-2.0

    Nil

    ADD FLOW CYTOMETRY

     

    add 5.0-7.0

     

     

    ADD OTHER INFECTIOUS MARKERS

     

     

    add 2.0- 5.0-

     

    ADD MYCOBACTERIAL CULTURE

     

     

    Add 10.00

     

    ADD CJD

     

     

     

    1.0

    ADD XANTHOCHROMIA

     

     

     

    1.0 (foil)

     

    Note, the ability to add further tests to a previously collected sample is dependent upon the volume provided.  Also, many tests (flow cytometry, cytology) can only be performed on fresh specimens.

     

    Other specialised testing (e.g. oligoclonal bands) will be performed using the supplied samples.

     

    Xanthochromia and CJD analysis cannot be performed on a blood-stained sample.

    Depending on the tests requested, 3-4 separate tubes will need to be provided. Note that the tubes are numbered so that order of collection can be identified – this order should be carefully followed, so that the optimal sample can be used for the requested tests. It is also important to consider the tests that are required, as this will determine how much CSF is needed in each tube. See the matrix below for details.

    TUBE 1 is used for Biochemistry and Flow Cytometry. (Centrifuged in the lab – the supernatant is used for Biochemistry analysis (glucose, protein) and the cellular pellet will be used for flow cytometry if required). If tube 3 is bloodstained, take an aliquot for microbiology.

    TUBE 2 is used for Cytology

    TUBE 3 is for Microbiological analysis (cell count, Gram stain and culture) and any further infectious testing (e.g. viral PCR, mycobacterial PCR and culture, cryptococcal antigen).

    TUBE 4 will be required only for;

    (a) Creutzfeld-Jacob Disease (CJD) testing OR

    (b) Xanthochromia analysis (wrap the tube in foil to prevent sample deterioration due to light).

     

    TESTS REQUIRED

    MINIMUM REQUIRED VOLUME (mL)

    TUBE 1

    TUBE 2

    TUBE 3

    TUBE 4

    CHEMISTRY

    1.0

         

    ADD FLOW CYTOMETRY

    Minimum 5.0-7.0

         

    CYTOLOGY

     

    1.0-3.0

       

    MICROBIOLOGY

       

    1.0-2.0

     

    ADD OTHER INFECTIOUS MARKERS

       

    add 2.0- 5.0-

     

    ADD MYCOBACTERIAL CULTURE

       

    Add 6.0-10.00

     

    ADD CJD

         

    1.0

    ADD XANTHOCHROMIA

         

    1.0 (foil)

     

    Note, the ability to add further tests to a previously collected sample is dependent upon the volume provided. Also, many tests (flow cytometry, cytology) can only be performed on fresh specimens.

     

    Other specialised testing (e.g. oligoclonal bands) will be performed using the supplied samples.

    Xanthochromia and CJD analysis cannot be performed on a blood-stained sample.

     

     


     Recent changes for CMV Serology

    Recent changes for CMV Serology

    Date
    Field
    Changed From
    Changed To
    18th January 2023
    Notes

    IgG and IgM, IgG Avidity Index performed

     

    IgG and IgM, IgG Avidity Index performed if IgM is positive

     

    18th January 2023
    Laboratory

    Microbiology

    Biochemistry


     Recent changes for Collagen Binding Assay

    Recent changes for Collagen Binding Assay

    Date
    Field
    Changed From
    Changed To
    11th June 2021
    Laboratory

    Referred Test

    Coagulation

    Request Group

    CBA

    Collection & Request Instructions

    Fill to label marker and mix by inversion.

    Do not use 1 mL citrate tubes.

    Fill to label marker and mix by inversion.

    Do not use 1 mL citrate tubes.

    Processing Instructions

    Specimens kept at room temperature, should be centrifuged ASAP and tested within 4 hours from time of specimen collection. If testing is not completed within 4 hours, plasma should be removed from cells and aliquoted (1mL) into Eppendorf tubes, 2 X 1 mL vials per CBA assay.

    Plasma may be frozen @ -20 C for up to 2 weeks or -70 C for up to 6 months. Frost free freezers should not be used.

    Specimens kept at room temperature, should be centrifuged ASAP and tested within 4 hours from time of specimen collection. If testing is not completed within 4 hours, plasma should be removed from cells and aliquoted (1mL) into Eppendorf tubes, 2 X 1 mL vials per CBA assay.

    Plasma may be frozen @ -20 C for up to 2 weeks or -70 C for up to 6 months. Frost free freezers should not be used.

    External Laboratory

    Austin Pathology

    Frequency

    Fortnightly


     Recent changes for Copper-Liver

    Recent changes for Copper-Liver

    Date
    Field
    Changed From
    Changed To
    10th May 2021
    External Laboratory

    Australian Clinical Labs (formerly Healthscope)

    Pacific Laboratory Medicine Services


     Recent changes for EBV Serology

    Recent changes for EBV Serology

    Date
    Field
    Changed From
    Changed To
    18th January 2023
    Laboratory

    Microbiology

    Biochemistry


     Recent changes for Faecal Calprotectin

    Recent changes for Faecal Calprotectin

    Date
    Field
    Changed From
    Changed To
    24th August 2022
    Volume (Adults)

    20 g

    Minimum/Paediatric Volume

    1 g

    Collection & Request Instructions

    Samples can be sent frozen.

    External Laboratory

    Monash Pathology

    Frequency

    Weekly

    Notes

     

    Contact

    Monash University, Eastern Health
    Department of Gastroenterology
    IBD Gastroenterology Laboratory
    Level 1, 5 Arnold St, Box Hill  Vic  3128
    Ph: 03 9895 4632
    Email: ehcs.ibd@monash.edu

    Link to test information and costs :

    http://www.med.monash.edu.au/ehcs/services/metabolite/calprotectindrsinformation.html

     

     

    24th August 2022
    Request Group

    CALPRO


     Recent changes for Flavivirus Serology

    Recent changes for Flavivirus Serology

    Date
    Field
    Changed From
    Changed To
    1st February 2023
    Alternative names

    Dengue fever serology, Kunjin serology, Murray Valley Encephalitis serology

    Dengue fever serology, Kunjin serology, Murray Valley Encephalitis serology, Japanese B encephalitis

    Request Group

    SDENG, OPCR, (SMVE, SJBE)

    SDENG, SMVE, SJBE

    31st January 2023
    Preferred Specimen Type

    Blood

    Blood
    Serum

    Preferred Container Type

    EDTA

    EDTA
    Serum tube with gel

    31st January 2023
    Request Group

    OPCR

    SDENG, OPCR, (SMVE, SJBE)

    Notes

    Procedure detects flaviviruses including Murray Valley encephalitis, dengue and kunjin viruses

    PCR testing detects flaviviruses including Murray Valley encephalitis, dengue and kunjin viruses. Dengue specific serology tests for IgG, IgM, and NS1 antigen


     Recent changes for Fluconazole

    Recent changes for Fluconazole

    Date
    Field
    Changed From
    Changed To
    14th October 2021
    Collection & Request Instructions

     

     

     

     

    Processing Instructions

    Spin, separate Plasma and freeze.

    Packed by certified personnel.

    Results:02-83829100 or 02-82829190

    Spin and separate Plasma.

     

    External Laboratory

    Sydpath Pathology, St Vincent's Hospital NSW

    Alfred Hospital


     Recent changes for Free PSA

    Recent changes for Free PSA

    Date
    Field
    Changed From
    Changed To
    21st June 2021
    External Laboratory

    Melbourne Pathology

    Method

    Free PSA is now performed in house at Box Hill Hospital.

    Chemical Pathologist approval is still required


     Recent changes for Hepatitis B Viral Load

    Recent changes for Hepatitis B Viral Load

    Date
    Field
    Changed From
    Changed To
    2nd February 2023
    Processing Instructions

    Perfomed by Abbott Alinity M

    Method

    Performed by Abbott Alinity M

    2nd February 2023
    Assay name

    Hepatitis B Quantitation

    Hepatitis B Viral Load

    Alternative names

    Hepatitis B Viral Load

    Hepatitis B Viral Load, Hep B

    Laboratory

    Referred Test

    Microbiology

    Request Group

    HEPBVL

    HBVL

    Processing Instructions

    Contact VIDRL 9342 9615

    Perfomed by Abbott Alinity M

    External Laboratory

    Victorian Infectious Diseases Reference Laboratory (V.I.D.R.L)


     Recent changes for IgG Subclasses

    Recent changes for IgG Subclasses

    Date
    Field
    Changed From
    Changed To
    13th September 2022
    External Laboratory

    Austin Pathology


     Recent changes for Infectious Mononucleosis Screen

    Recent changes for Infectious Mononucleosis Screen

    Date
    Field
    Changed From
    Changed To
    30th March 2022
    Preferred Specimen Type

    Serum

    Serum
    Blood

    Optional Specimen Type

    Plasma

    Preferred Container Type

    Serum tube with gel

    Serum tube with gel
    EDTA
    EDTA - Paediatric


     Recent changes for Infliximab

    Recent changes for Infliximab

    Date
    Field
    Changed From
    Changed To
    5th April 2022
    Alternative names

    Inflectra, Remicade

    Volume (Adults)

    2 mL

    Processing Instructions

    After centifugation the serum must be aliquoted into the special aliquot tube provided and freeze.

    Store at 4 C.

     

    External Laboratory

    South Western Area Pathology,Liverpool Hospital

    Dorevitch Pathology

    External Transport Instructions

    Samples to be batched and sent  to SSWAPs frozen

    Send to Testing Laboratory at 4 C

    Frequency

    Monthly

    Weekly


     Recent changes for Iron - Liver

    Recent changes for Iron - Liver

    Date
    Field
    Changed From
    Changed To
    10th May 2021
    Collection & Request Instructions

    Liver biopsy specimen to be placed directly into a clear, dry container and delivered to the laboraory as soon as possible. Minimum sample size for accurate analysis is 1-2 mg.

    Liver biopsy specimen to be placed directly into a clear, dry container and delivered to the laboraory as soon as possible. Minimum sample size for accurate analysis is 1-2 mg.

    External Laboratory

    Australian Clinical Labs (formerly Healthscope)

    Pacific Laboratory Medicine Services

    Notes

    Hepatic Iron Index

    Hepatic Iron Concentration

     


     

    Age in years

    Values > 2 indicate haemochromatosis

    Liver biopsy specimen to be placed directly into a clear, dry container and delivered to the laboraory as soon as possible. Minimum sample size for accurate analysis is 1-2 mg.

     

    Hepatic Iron Index

    Hepatic Iron Concentration

     

     

    Age in years

    Values > 2 indicate haemochromatosis

    Liver biopsy specimen to be placed directly into a clear, dry container and delivered to the laboraory as soon as possible. Minimum sample size for accurate analysis is 1-2 mg.


     Recent changes for Itraconazole

    Recent changes for Itraconazole

    Date
    Field
    Changed From
    Changed To
    14th July 2022
    Volume (Adults)

    2 mL

    5 mL

    Minimum/Paediatric Volume

    1 mL

    Preferred Container Type

    Lithium heparin no gel

    EDTA

    Processing Instructions

    Spin, separate and freeze plasma.

    Spin and separate plasma.

    External Laboratory

    Women's & Children's Hospital (Adelaide)

    Alfred Hospital

    External Transport Instructions

    Packed by certified personnal - transport frozen.

    Results : 08-81617000, 08-81616732.

    Frequency

    Monthly

    Twice weekly

    Notes

    Dose information should be provided and length of therapy must be >5 days.


     Recent changes for Karyotype/Microarray - TISSUE

    Recent changes for Karyotype/Microarray - TISSUE

    Date
    Field
    Changed From
    Changed To
    6th March 2022
    Assay name

    Cytogenetics / Karyotype - TISSUE

    Karyotype/Microarray - TISSUE

    14th December 2021
    Assay name

    CytogeneticsKaryotype - TISSUE

    Cytogenetics / Karyotype - TISSUE

    14th December 2021
    Assay name

    CYTOGENETICS - TISSUE

    CytogeneticsKaryotype - TISSUE

    Alternative names

    KARYOTYPE CHROMOSOME STUDIES - TISSUE TISSUE CULTURE MOLECULAR KARYOTYPE MICROARRAY

    KARYOTYPE CHROMOSOME STUDIES - TISSUE TISSUE CULTURE MOLECULAR KARYOTYPE MICROARRAY FIBROBLAST CULTURE

    14th December 2021
    Assay name

    CYTOGENETICS - TISSUE NON MALIGNANT (CONSTITUTIONAL)

    CYTOGENETICS - TISSUE

    Frequency

    Weekly

    Daily


     Recent changes for Lamotrigine

    Recent changes for Lamotrigine

    Date
    Field
    Changed From
    Changed To
    14th July 2022
    Alternative names

    Lamictal

    Volume (Adults)

    2 mL

    3 mL

    Minimum/Paediatric Volume

    1 mL

    3 mL

    Processing Instructions

    Spin aliquot plasma. (Send either plasma or whole blood)

    Spin & aliquot plasma

    External Laboratory

    Australian Clinical Labs (formerly Healthscope)

    Alfred Hospital


     Recent changes for Leucocyte Immunophenotyping

    Recent changes for Leucocyte Immunophenotyping

    Date
    Field
    Changed From
    Changed To
    15th October 2021
    Notes

    Samples must be marked 'URGENT', must reach the laboratory as soon as possible. 

    All out-of-hours testing requires notification.

    Time and date of collection must be recorded on request form.

    Whole blood - 1 x EDTA sample, do not spin. Store at room temperature. An FBE must also be requested,  and needs a separate EDTA sample.

    Bone marrow aspirate - Sample into  EDTA tube

    TissueFresh (if to be shared with other departments for other tests eg. Histopathology or Microbiology) or RPMI fluid (especially if specimen is taken out of hours). This fluid is obtainable from: BH Lab 9895 3884, MH Lab: 9871 3572 or AH Lab: 9764 6136.

    CSF/other fluids

    Depending on the tests requested, 3-4 separate tubes will need to be provided. Note that the tubes are numbered so that order of collection can be identified – this order should be carefully followed, so that the optimal sample can be used for the requested tests. It is also important to consider the tests that are required, as this will determine how much CSF is needed in each tube. See the matrix below for details.

    TUBE 1 is used for Biochemistry and Flow Cytometry. (Centrifuged in the lab – the supernatant is used for Biochemistry analysis (glucose, protein) and the cellular pellet will be used for flow cytometry if required). If tube 3 is bloodstained, take an aliquot for microbiology.

    TUBE 2 is used for Cytology

    TUBE 3 is for Microbiological analysis (cell count, Gram stain and culture) and any further infectious testing (e.g. viral PCR, mycobacterial PCR and culture, cryptococcal antigen).

    TUBE 4 will be required only for;

    (a) Creutzfeld-Jacob Disease (CJD) testing OR

    (b) Xanthochromia analysis (wrap the tube in foil to prevent sample deterioration due to light).

     

    TESTS REQUIRED

    MINIMUM REQUIRED VOLUME (mL)

    TUBE 1

    TUBE 2

    TUBE 3

    TUBE 4

    CHEMISTRY

    1.0

     

     

     

    ADD FLOW CYTOMETRY

    Minimum 5.0-7.0

     

     

     

    CYTOLOGY

     

    1.0-3.0

     

     

    MICROBIOLOGY

     

     

    1.0-2.0

     

    ADD OTHER INFECTIOUS MARKERS

     

     

    add 2.0- 5.0-

     

    ADD MYCOBACTERIAL CULTURE

     

     

    Add 6.0-10.00

     

    ADD CJD

     

     

     

    1.0

    ADD XANTHOCHROMIA

     

     

     

    1.0 (foil)

     

    Note, the ability to add further tests to a previously collected sample is dependent upon the volume provided. Also, many tests (flow cytometry, cytology) can only be performed on fresh specimens.

     

    Other specialised testing (e.g. oligoclonal bands) will be performed using the supplied samples.

    Xanthochromia and CJD analysis cannot be performed on a blood-stained sample

    Samples must be marked 'URGENT', must reach the laboratory as soon as possible. 

    All out-of-hours testing requires notification.

    Time and date of collection must be recorded on request form.

    Whole blood - 1 x EDTA sample, do not spin. Store at room temperature. An FBE and BLOOD FILM must also be requested,  and needs a separate EDTA sample.

    Bone marrow aspirate - Sample into  EDTA tube

    TissueFresh (if to be shared with other departments for other tests eg. Histopathology or Microbiology) or RPMI fluid (especially if specimen is taken out of hours). This fluid is obtainable from: BH Lab 9895 3884, MH Lab: 9871 3572 or AH Lab: 9764 6136.

    CSF/other fluids

    Depending on the tests requested, 3-4 separate tubes will need to be provided. Note that the tubes are numbered so that order of collection can be identified – this order should be carefully followed, so that the optimal sample can be used for the requested tests. It is also important to consider the tests that are required, as this will determine how much CSF is needed in each tube. See the matrix below for details.

    TUBE 1 is used for Biochemistry and Flow Cytometry. (Centrifuged in the lab – the supernatant is used for Biochemistry analysis (glucose, protein) and the cellular pellet will be used for flow cytometry if required). If tube 3 is bloodstained, take an aliquot for microbiology.

    TUBE 2 is used for Cytology

    TUBE 3 is for Microbiological analysis (cell count, Gram stain and culture) and any further infectious testing (e.g. viral PCR, mycobacterial PCR and culture, cryptococcal antigen).

    TUBE 4 will be required only for;

    (a) Creutzfeld-Jacob Disease (CJD) testing OR

    (b) Xanthochromia analysis (wrap the tube in foil to prevent sample deterioration due to light).

     

    TESTS REQUIRED

    MINIMUM REQUIRED VOLUME (mL)

    TUBE 1

    TUBE 2

    TUBE 3

    TUBE 4

    CHEMISTRY

    1.0

     

     

     

    ADD FLOW CYTOMETRY

    Minimum 5.0-7.0

     

     

     

    CYTOLOGY

     

    1.0-3.0

     

     

    MICROBIOLOGY

     

     

    1.0-2.0

     

    ADD OTHER INFECTIOUS MARKERS

     

     

    add 2.0- 5.0-

     

    ADD MYCOBACTERIAL CULTURE

     

     

    Add 6.0-10.00

     

    ADD CJD

     

     

     

    1.0

    ADD XANTHOCHROMIA

     

     

     

    1.0 (foil)

     

    Note, the ability to add further tests to a previously collected sample is dependent upon the volume provided. Also, many tests (flow cytometry, cytology) can only be performed on fresh specimens.

     

    Other specialised testing (e.g. oligoclonal bands) will be performed using the supplied samples.

    Xanthochromia and CJD analysis cannot be performed on a blood-stained sample

    14th October 2021
    Notes

     

     

    Samples must be marked 'URGENT', must reach the laboratory as soon as possible. 

    All out-of-hours testing requires notification.

    Time and date of collection must be recorded on request form.

    Whole blood - 1 x EDTA sample, do not spin. Store at room temperature. An FBE must also be requested,  and needs a separate EDTA sample.

    Bone marrow aspirate - Sample into  EDTA tube

    TissueFresh (if to be shared with other departments for other tests eg. Histopathology or Microbiology) or RPMI fluid (especially if specimen is taken out of hours). This fluid is obtainable from: BH Lab 9895 3884, MH Lab: 9871 3572 or AH Lab: 9764 6136.

    CSF/other fluids - at least 2mL is preferred

    Depending on the tests requested, 3-4 separate tubes will need to be provided. Note that the tubes are numbered so that order of collection can be identified – this order should be carefully followed, so that the optimal sample can be used for the requested tests. It is also important to consider the tests that are required, as this will determine how much CSF is needed in each tube. See the matrix below for details.

    TUBE 1 is used for Biochemistry and Flow Cytometry. (Centrifuged in the lab – the supernatant is used for Biochemistry analysis (glucose, protein) and the cellular pellet will be used for flow cytometry if required). If tube 3 is bloodstained, take an aliquot for microbiology.

    TUBE 2 is used for Cytology

    TUBE 3 is for Microbiological analysis (cell count, Gram stain and culture) and any further infectious testing (e.g. viral PCR, mycobacterial PCR and culture, cryptococcal antigen).

    TUBE 4 will be required only for;

    (a) Creutzfeld-Jacob Disease (CJD) testing OR

    (b) Xanthochromia analysis (wrap the tube in foil to prevent sample deterioration due to light).

     

    TESTS REQUIRED

    MINIMUM REQUIRED VOLUME (mL)

    TUBE 1

    TUBE 2

    TUBE 3

    TUBE 4

    CHEMISTRY

    1.0

     

     

     

    ADD FLOW CYTOMETRY

    Minimum 5.0-7.0

     

     

     

    CYTOLOGY

     

    1.0-3.0

     

     

    MICROBIOLOGY

     

     

    1.0-2.0

     

    ADD OTHER INFECTIOUS MARKERS

     

     

    add 2.0- 5.0-

     

    ADD MYCOBACTERIAL CULTURE

     

     

    Add 6.0-10.00

     

    ADD CJD

     

     

     

    1.0

    ADD XANTHOCHROMIA

     

     

     

    1.0 (foil)

     

    Note, the ability to add further tests to a previously collected sample is dependent upon the volume provided. Also, many tests (flow cytometry, cytology) can only be performed on fresh specimens.

     

    Other specialised testing (e.g. oligoclonal bands) will be performed using the supplied samples.

    Xanthochromia and CJD analysis cannot be performed on a blood-stained sample

     

     

    Samples must be marked 'URGENT', must reach the laboratory as soon as possible. 

    All out-of-hours testing requires notification.

    Time and date of collection must be recorded on request form.

    Whole blood - 1 x EDTA sample, do not spin. Store at room temperature. An FBE must also be requested,  and needs a separate EDTA sample.

    Bone marrow aspirate - Sample into  EDTA tube

    TissueFresh (if to be shared with other departments for other tests eg. Histopathology or Microbiology) or RPMI fluid (especially if specimen is taken out of hours). This fluid is obtainable from: BH Lab 9895 3884, MH Lab: 9871 3572 or AH Lab: 9764 6136.

    CSF/other fluids

    Depending on the tests requested, 3-4 separate tubes will need to be provided. Note that the tubes are numbered so that order of collection can be identified – this order should be carefully followed, so that the optimal sample can be used for the requested tests. It is also important to consider the tests that are required, as this will determine how much CSF is needed in each tube. See the matrix below for details.

    TUBE 1 is used for Biochemistry and Flow Cytometry. (Centrifuged in the lab – the supernatant is used for Biochemistry analysis (glucose, protein) and the cellular pellet will be used for flow cytometry if required). If tube 3 is bloodstained, take an aliquot for microbiology.

    TUBE 2 is used for Cytology

    TUBE 3 is for Microbiological analysis (cell count, Gram stain and culture) and any further infectious testing (e.g. viral PCR, mycobacterial PCR and culture, cryptococcal antigen).

    TUBE 4 will be required only for;

    (a) Creutzfeld-Jacob Disease (CJD) testing OR

    (b) Xanthochromia analysis (wrap the tube in foil to prevent sample deterioration due to light).

     

    TESTS REQUIRED

    MINIMUM REQUIRED VOLUME (mL)

    TUBE 1

    TUBE 2

    TUBE 3

    TUBE 4

    CHEMISTRY

    1.0

     

     

     

    ADD FLOW CYTOMETRY

    Minimum 5.0-7.0

     

     

     

    CYTOLOGY

     

    1.0-3.0

     

     

    MICROBIOLOGY

     

     

    1.0-2.0

     

    ADD OTHER INFECTIOUS MARKERS

     

     

    add 2.0- 5.0-

     

    ADD MYCOBACTERIAL CULTURE

     

     

    Add 6.0-10.00

     

    ADD CJD

     

     

     

    1.0

    ADD XANTHOCHROMIA

     

     

     

    1.0 (foil)

     

    Note, the ability to add further tests to a previously collected sample is dependent upon the volume provided. Also, many tests (flow cytometry, cytology) can only be performed on fresh specimens.

     

    Other specialised testing (e.g. oligoclonal bands) will be performed using the supplied samples.

    Xanthochromia and CJD analysis cannot be performed on a blood-stained sample

    14th October 2021
    Alternative names

    CD4/CD8 Counts, Cell Surface Markers, FACS Analysis, Flow Cytometry

    Cell Surface Markers, FACS Analysis, Flow Cytometry, Immunophenotyping

    Request Group

    FLOW

    LYMP, LEUK

    Collection & Request Instructions

    Sample store at Room temperature. Samples are not stable for more than 24 hours. Hence they must be received by the Flow Cytometry lab at Box Hill within normal business hours Monday to Friday.

    Cutoff for outpatient sample collection on Fridays is at 11.30 AM and MUST reach the laboratory before 4.00PM.

    Flow cytometric analysis of cells stained by immunofluorescence with a range of antibodies against lymphoid and myeloid markers.

    The panel of markers used will be appropriate to the clinical comments supplied , or as arranged by prior consultation.

    Sample store at Room temperature. Samples are not stable for more than 24 hours. Hence they must be received by the Flow Cytometry lab at Box Hill within normal business hours Monday to Friday.

    Cut-off for outpatient sample collection on Fridays is at 11.30 AM and MUST reach the laboratory before 4.00PM.

    Flow cytometric analysis of cells stained by immunofluorescence with a range of antibodies against lymphoid and myeloid markers.

    The panel of markers used will be appropriate to the panel selected and the clinical comments supplied , or as arranged by prior consultation

    Notes

     

     

    Samples must be marked 'URGENT', must reach the laboratory as soon as possible. 

    All out-of-hours testing requires notification.

    Time and date of collection must be recorded on request form.

    Whole blood - 1 x EDTA sample, do not spin. Store at room temperature. An FBE must also be requested, needs a separate EDTA sample.

    Bone marrow aspirate - Sample into  EDTA tube

    TissueFresh (if to be shared with other departments for other tests eg. Histopathology or Microbiology) or RPMI fluid (especially if specimen is taken out of hours). This fluid is obtainable from: BH Lab 9895 3884, MH Lab: 9871 3572 or AH Lab: 9764 6136.

    CSF/other fluids - at least 2mL is preferred

    Collect CSF into three separate, sterile, screw-capped tubes provided (labelled 1,2 & 3)

    Tube 1 is for Biochemistry (for Protein and Glucose) 0.5ml (10 drops) required 

    Tube 2 for Cytology 1.0ml (20 drops)  and/or Flow Cytometry (6ml)

    For Flow Cytometry a minimum of 6ml is required  in Tube 2

    For Flow Cytometry and Cytology a minimum of 7ml is required in Tube 2     

    Tube 3 for Microbiology upto 10ml (Full tube)

    If querying CJD the laboratory requires a 4th unopened tube containing at least 1ml of non-bloodstained CSF.

    Xanthochromia investigation requires a 4th tube containing 1ml of CSF .

     

     

    Samples must be marked 'URGENT', must reach the laboratory as soon as possible. 

    All out-of-hours testing requires notification.

    Time and date of collection must be recorded on request form.

    Whole blood - 1 x EDTA sample, do not spin. Store at room temperature. An FBE must also be requested,  and needs a separate EDTA sample.

    Bone marrow aspirate - Sample into  EDTA tube

    TissueFresh (if to be shared with other departments for other tests eg. Histopathology or Microbiology) or RPMI fluid (especially if specimen is taken out of hours). This fluid is obtainable from: BH Lab 9895 3884, MH Lab: 9871 3572 or AH Lab: 9764 6136.

    CSF/other fluids - at least 2mL is preferred

    Depending on the tests requested, 3-4 separate tubes will need to be provided. Note that the tubes are numbered so that order of collection can be identified – this order should be carefully followed, so that the optimal sample can be used for the requested tests. It is also important to consider the tests that are required, as this will determine how much CSF is needed in each tube. See the matrix below for details.

    TUBE 1 is used for Biochemistry and Flow Cytometry. (Centrifuged in the lab – the supernatant is used for Biochemistry analysis (glucose, protein) and the cellular pellet will be used for flow cytometry if required). If tube 3 is bloodstained, take an aliquot for microbiology.

    TUBE 2 is used for Cytology

    TUBE 3 is for Microbiological analysis (cell count, Gram stain and culture) and any further infectious testing (e.g. viral PCR, mycobacterial PCR and culture, cryptococcal antigen).

    TUBE 4 will be required only for;

    (a) Creutzfeld-Jacob Disease (CJD) testing OR

    (b) Xanthochromia analysis (wrap the tube in foil to prevent sample deterioration due to light).

     

    TESTS REQUIRED

    MINIMUM REQUIRED VOLUME (mL)

    TUBE 1

    TUBE 2

    TUBE 3

    TUBE 4

    CHEMISTRY

    1.0

     

     

     

    ADD FLOW CYTOMETRY

    Minimum 5.0-7.0

     

     

     

    CYTOLOGY

     

    1.0-3.0

     

     

    MICROBIOLOGY

     

     

    1.0-2.0

     

    ADD OTHER INFECTIOUS MARKERS

     

     

    add 2.0- 5.0-

     

    ADD MYCOBACTERIAL CULTURE

     

     

    Add 6.0-10.00

     

    ADD CJD

     

     

     

    1.0

    ADD XANTHOCHROMIA

     

     

     

    1.0 (foil)

     

    Note, the ability to add further tests to a previously collected sample is dependent upon the volume provided. Also, many tests (flow cytometry, cytology) can only be performed on fresh specimens.

     

    Other specialised testing (e.g. oligoclonal bands) will be performed using the supplied samples.

    Xanthochromia and CJD analysis cannot be performed on a blood-stained sample

    20th July 2021
    Assay name

    Leucocyte Immunophenotyping

    T Cell Subsets

    Alternative names

    CD4/CD8 Counts, Cell Surface Markers, FACS Analysis, Flow Cytometry

    CD2, CD3, CD4, CD8

    Preferred Container Type

    EDTA
    Sterile Jar

    EDTA
    Lithium heparin no gel

    Collection & Request Instructions

    Sample store at Room temperature. Samples are not stable for more than 24 hours. Hence they must be received by the Flow Cytometry lab at Box Hill within normal business hours Monday to Friday.

    Cutoff for outpatient sample collection on Fridays is at 11.30 AM and MUST reach the laboratory before 4.00PM.

    Flow cytometric analysis of cells stained by immunofluorescence with a range of antibodies against lymphoid and myeloid markers.

    The panel of markers used will be appropriate to the clinical comments supplied , or as arranged by prior consultation.

    The samples are not stable for more than 24 hours. Hence all samples must be recieved in the laboratory at Box Hill  Monday to Friday within business hours.

    Cutoff for outpatient sample collection on Fridays is at 11.30 AM and MUST reach the laboratory before 4.00PM.

    Processing Instructions

    Prefer EDTA sample.

    Do not spin, store at room temperature.

    Notes

     

     

    Samples must be marked 'URGENT', must reach the laboratory as soon as possible. 

    All out-of-hours testing requires notification.

    Time and date of collection must be recorded on request form.

    Whole blood - 1 x EDTA sample, do not spin. Store at room temperature. An FBE must also be requested, needs a separate EDTA sample.

    Bone marrow aspirate - Sample into  EDTA tube

    TissueFresh (if to be shared with other departments for other tests eg. Histopathology or Microbiology) or RPMI fluid (especially if specimen is taken out of hours). This fluid is obtainable from: BH Lab 9895 3884, MH Lab: 9871 3572 or AH Lab: 9764 6136.

    CSF/other fluids - at least 2mL is preferred

    Collect CSF into three separate, sterile, screw-capped tubes provided (labelled 1,2 & 3)

    Tube 1 is for Biochemistry (for Protein and Glucose) 0.5ml (10 drops) required 

    Tube 2 for Cytology 1.0ml (20 drops)  and/or Flow Cytometry (6ml)

    For Flow Cytometry a minimum of 6ml is required  in Tube 2

    For Flow Cytometry and Cytology a minimum of 7ml is required in Tube 2     

    Tube 3 for Microbiology upto 10ml (Full tube)

    If querying CJD the laboratory requires a 4th unopened tube containing at least 1ml of non-bloodstained CSF.

    Xanthochromia investigation requires a 4th tube containing 1ml of CSF .

    Used to monitor T-cell numbers

    Preferred EDTA sample. Do not spin, store at room temperature.

    19th July 2021
    Collection & Request Instructions

    Sample store at Room temperature. Samples are not stable for more than 24 hours. Hence they must be recieved by the Flow Cytometry lab at Box Hill within normal business hours Monday to Friday.

    Cutoff for outpatirnt sample collection on Fridays is at 12:00 PM and MUST reach the laboratory by 4.00PM.

    Flow cytometric analysis of cells stained by immunofluorescence with a range of antibodies against lymphoid and myeloid markers.

    The panel of markers used will be appropriate to the clinical comments supplied , or as arranged by prior consultation.

    Sample store at Room temperature. Samples are not stable for more than 24 hours. Hence they must be received by the Flow Cytometry lab at Box Hill within normal business hours Monday to Friday.

    Cutoff for outpatient sample collection on Fridays is at 11.30 AM and MUST reach the laboratory before 4.00PM.

    Flow cytometric analysis of cells stained by immunofluorescence with a range of antibodies against lymphoid and myeloid markers.

    The panel of markers used will be appropriate to the clinical comments supplied , or as arranged by prior consultation.

    19th July 2021
    Collection & Request Instructions

    Sample store at Room temperature. Samples are not stable for more than 24 hours. Hence they must be recieved by the Flow Cytometry lab at Box Hill within normal business hours Monday to Friday

    Flow cytometric analysis of cells stained by immunofluorescence with a range of antibodies against lymphoid and myeloid markers.

    The panel of markers used will be appropriate to the clinical comments supplied , or as arranged by prior consultation.

    Sample store at Room temperature. Samples are not stable for more than 24 hours. Hence they must be recieved by the Flow Cytometry lab at Box Hill within normal business hours Monday to Friday.

    Cutoff for outpatirnt sample collection on Fridays is at 12:00 PM and MUST reach the laboratory by 4.00PM.

    Flow cytometric analysis of cells stained by immunofluorescence with a range of antibodies against lymphoid and myeloid markers.

    The panel of markers used will be appropriate to the clinical comments supplied , or as arranged by prior consultation.

    Notes

     

     

    • Samples must be marked 'URGENT', must reach the laboratory as soon as possible. 
    • All out-of-hours testing requires notification.
    • Time and date of collection must be recorded on request form.

      Whole blood - 1 x EDTA sample, do not spin. Store at room temperature. An FBE must also be requested, needs a separate EDTA sample.

      Bone marrow aspirate - Sample into  EDTA tube

      TissueFresh (if to be shared with other departments for other tests eg. Histopathology or Microbiology) or RPMI fluid (especially if specimen is taken out of hours). This fluid is obtainable from: BH Lab 9895 3884, MH Lab: 9871 3572 or AH Lab: 9764 6136.

    • CSF/other fluids - at least 2mL is preferred

    • Collect CSF into three separate, sterile, screw-capped tubes provided (labelled 1,2 & 3)

    • Tube 1 is for Biochemistry (for Protein and Glucose) 0.5ml (10 drops) required 
    • Tube 2 for Cytology 1.0ml (20 drops)  and/or Flow Cytometry (6ml)
      • For Flow Cytometry a minimum of 6ml is required  in Tube 2
      • For Flow Cytometry and Cytology a minimum of 7ml is required in Tube 2     
    • Tube 3 for Microbiology upto 10ml (Full tube)

      If querying CJD the laboratory requires a 4th unopened tube containing at least 1ml of non-bloodstained CSF.

      Xanthochromia investigation requires a 4th tube containing 1ml of CSF .

     

     

    Samples must be marked 'URGENT', must reach the laboratory as soon as possible. 

    All out-of-hours testing requires notification.

    Time and date of collection must be recorded on request form.

    Whole blood - 1 x EDTA sample, do not spin. Store at room temperature. An FBE must also be requested, needs a separate EDTA sample.

    Bone marrow aspirate - Sample into  EDTA tube

    TissueFresh (if to be shared with other departments for other tests eg. Histopathology or Microbiology) or RPMI fluid (especially if specimen is taken out of hours). This fluid is obtainable from: BH Lab 9895 3884, MH Lab: 9871 3572 or AH Lab: 9764 6136.

    CSF/other fluids - at least 2mL is preferred

    Collect CSF into three separate, sterile, screw-capped tubes provided (labelled 1,2 & 3)

    Tube 1 is for Biochemistry (for Protein and Glucose) 0.5ml (10 drops) required 

    Tube 2 for Cytology 1.0ml (20 drops)  and/or Flow Cytometry (6ml)

    For Flow Cytometry a minimum of 6ml is required  in Tube 2

    For Flow Cytometry and Cytology a minimum of 7ml is required in Tube 2     

    Tube 3 for Microbiology upto 10ml (Full tube)

    If querying CJD the laboratory requires a 4th unopened tube containing at least 1ml of non-bloodstained CSF.

    Xanthochromia investigation requires a 4th tube containing 1ml of CSF .


     Recent changes for Linezolid

    Recent changes for Linezolid

    Date
    Field
    Changed From
    Changed To
    14th July 2022
    Volume (Adults)

    2 mL

    3 mL

    Minimum/Paediatric Volume

    3 mL

    Optional Container Type

    Lithium heparin no gel

    Collection & Request Instructions

    Deliver to laboratory ASAP.

    A separate unopened specimen is required for this test.

    Processing Instructions

    Spin, separate and freeze plasma.

    External Laboratory

    Sydpath Pathology, St Vincent's Hospital NSW

    Alfred Hospital

    External Transport Instructions

    Send at 4 C.

    Frequency

    As required

    Weekly

    Method

    Results: Clinical Pharmacology on 02 8382 9184.


     Recent changes for Lupus Anticoagulant Screen

    Recent changes for Lupus Anticoagulant Screen

    Date
    Field
    Changed From
    Changed To
    3rd May 2021
    External Transport Instructions

    Samples can be transported frozen to the laboratory if the facilities are available to double spin and aliquot these sample. This has to be done within 4 hours.

    Unspun sample is stable for up to 4 hours and must be transport to laboratory immediately. (Sample must be double spun, aliquoted and frozen within 4 hours from time of collection).

    Samples can be transported frozen to the laboratory if the facilities are available to double spin and aliquot these samples. This has to be performed within 4 hours of collection.

    Unspun sample is stable for up to 4 hours and must be transport to laboratory immediately. (Sample must be double spun, aliquoted and frozen within 4 hours from time of collection).

    3rd May 2021
    Collection & Request Instructions

    Notification of anticoagulant therapy is essential

    One (1x) Sodium citrate tubes is required. Do not use 1 ml citrate tubes.

    Filled to label marker and mixed by inversion

    The patient must not be receiving heparin

    Transport to laboratory immediately. (Sample must be double spun and frozen within 4 hours from time of collection).

    Tests performed:

    INR, APTT, APTT Mix, Silica Clotting Time and Dilute Russell's Viper venom time (LA screen/Confirm).

     

    Notification of anticoagulant therapy is essential

    One (1x) Sodium citrate tubes is required. Do not use 1 ml citrate tubes.

    Filled to label marker and mixed by inversion

    The patient must not be receiving heparin

    Unspun sample is stable for up to 4 hours and must be transport to laboratory immediately. (Sample must be double spun, aliquoted and frozen within 4 hours from time of collection).

     

    Tests performed:

    INR, APTT, APTT Mix, Silica Clotting Time and Dilute Russell's Viper venom time (LA screen/Confirm).

     

    Processing Instructions

    Transport sample to laboratory within 4 hours of collection.

    The sample MUST be double spun and frozen within the 4 hour time limit.

    Unspun sample is stable for up to 4 hours and must be transport to laboratory immediately. (Sample must be double spun, aliquoted and frozen within 4 hours from time of collection).

    External Transport Instructions

    Samples can be transported frozen to the laboratory if the facilities are available to double spin and aliquot these sample. This has to be done within 4 hours.

    Unspun sample is stable for up to 4 hours and must be transport to laboratory immediately. (Sample must be double spun, aliquoted and frozen within 4 hours from time of collection).

    3rd May 2021
    Collection & Request Instructions

    Notification of anticoagulant therapy is essential

    One (1x) Sodium citrate tubes is required. Do not use 1 ml citrate tubes.

    Filled to label marker and mixed by inversion

    The patient must not be receiving heparin

    Transport to laboratory immediately. (maximum of 4 hours from time of collection).

    Tests performed:

    INR, APTT, APTT Mix, Silica Clotting Time and Dilute Russell's Viper venom time (LA screen/Confirm).

     

    Notification of anticoagulant therapy is essential

    One (1x) Sodium citrate tubes is required. Do not use 1 ml citrate tubes.

    Filled to label marker and mixed by inversion

    The patient must not be receiving heparin

    Transport to laboratory immediately. (Sample must be double spun and frozen within 4 hours from time of collection).

    Tests performed:

    INR, APTT, APTT Mix, Silica Clotting Time and Dilute Russell's Viper venom time (LA screen/Confirm).

     

    Processing Instructions

    Transport sample to laboratory within 4 hours of collection.

    The sample MUST be double spun and frozen within the 4 hour time limit.


     Recent changes for Lymphocyte subsets

    Recent changes for Lymphocyte subsets

    Date
    Field
    Changed From
    Changed To
    14th October 2021
    Alternative names

    CD3 count, CD4 count, CD8 count, CD4:CD8 ratio CD19 count, CD56 count, flow cytometry

    14th October 2021
    Alternative names

    CD19 count & CD56 count, CD4 count, CD4 count, CD4:CD8 ratio Lymphocyte subsets include: CD3 count, CD4:CD8 ratio, CD8 count, CD8 count, T subsets include: CD3 count

    T subsets include: CD3 count, CD4 count, CD8 count, CD4:CD8 ratio Lymphocyte subsets include: CD3 count, CD4 count, CD8 count, CD4:CD8 ratio, CD19 count & CD56 count Flow Cytometry

    Collection & Request Instructions

    Sample store at Room temperature. Samples are not stable for more than 24 hours. Hence they must be received by the Flow Cytometry lab at Box Hill within normal business hours Monday to Friday.

    Cut-off for outpatient sample collection on Fridays is at 11.30 AM and MUST reach the laboratory before 4.00PM.

    Flow cytometric analysis of cells stained by immunofluorescence with a range of antibodies against lymphoid and myeloid markers.

    The panel of markers used will be appropriate to the panel selected and the clinical comments supplied, or as arranged by prior consultation.

    Sample store at ROOM TEMPERATURE. Samples are not stable for more than 24 hours. Hence they must be received by the Flow Cytometry lab at Box Hill within normal business hours Monday to Friday.

    Cut-off for outpatient sample collection on Fridays is at 11.30 AM and MUST reach the laboratory before 4.00PM.

    Flow cytometric analysis of cells stained by immunofluorescence with a range of antibodies against lymphoid and myeloid markers.

    The panel of markers used will be appropriate to the panel selected and the clinical comments supplied, or as arranged by prior consultation.

    14th October 2021
    Collection & Request Instructions

    The samples are not stable for more than 24 hours. Hence all samples must be recieved in the laboratory at Box Hill  Monday to Friday within business hours.

    Cutoff for outpatient sample collection on Fridays is at 11.30 AM and MUST reach the laboratory before 4.00PM.

     

    Sample store at Room temperature. Samples are not stable for more than 24 hours. Hence they must be received by the Flow Cytometry lab at Box Hill within normal business hours Monday to Friday.

    Cut-off for outpatient sample collection on Fridays is at 11.30 AM and MUST reach the laboratory before 4.00PM.

    Flow cytometric analysis of cells stained by immunofluorescence with a range of antibodies against lymphoid and myeloid markers.

    The panel of markers used will be appropriate to the panel selected and the clinical comments supplied, or as arranged by prior consultation.

    Frequency

    Daily

    Notes

    Used to monitor T-cell numbers

    Preferred EDTA sample. Do not spin, store at room temperature.

    Samples must be marked 'URGENT', must reach the laboratory as soon as possible. 

    All out-of-hours testing requires notification.

    Time and date of collection must be recorded on request form.

    Whole blood - 1 x EDTA sample, do not spin. Store at room temperature. An FBE must also be requested, and needs a separate EDTA sample.

    14th October 2021
    Assay name

    T Cell Subsets

    Lymphocyte subsets

    Alternative names

    CD2, CD3, CD4, CD8

    T subsets include: CD3 count, CD4 count, CD8 count, CD4:CD8 ratio Lymphocyte subsets include: CD3 count, CD4 count, CD8 count, CD4:CD8 ratio, CD19 count & CD56 count

    Request Group

    FLOW

    TSUB & LSUB

    20th July 2021
    Processing Instructions

    Prefer EDTA sample.

    Do not spin, store at room temperature.

    Prefer EDTA sample.

    Do not spin, store at room temperature.

    20th July 2021
    Notes

    Used to monitor T-cell numbers

    Preferred EDTA sample. Do not spin, store at room temperature.

    Used to monitor T-cell numbers

    Preferred EDTA sample. Do not spin, store at room temperature.

    20th July 2021
    Collection & Request Instructions

    The samples are not stable for more than 24 hours. Hence all samples must be recieved in the laboratory at Box Hill  Monday to Friday within business hours.

    The samples are not stable for more than 24 hours. Hence all samples must be recieved in the laboratory at Box Hill  Monday to Friday within business hours.

    Cutoff for outpatient sample collection on Fridays is at 11.30 AM and MUST reach the laboratory before 4.00PM.

     

    Processing Instructions

    Prefer EDTA sample.

    Do not spin, store at room temperature.

    Prefer EDTA sample.

    Do not spin, store at room temperature.

    Notes

    Used to monitor T-cell numbers

    Preferred EDTA sample. Do not spin, store at room temperature.

    Used to monitor T-cell numbers

    Preferred EDTA sample. Do not spin, store at room temperature.


     Recent changes for Measles Serology

    Recent changes for Measles Serology

    Date
    Field
    Changed From
    Changed To
    18th January 2023
    Laboratory

    Microbiology

    Biochemistry

    Request Group

    SMEA

    MEA

    Frequency

    Daily

    Twice weekly


     Recent changes for Methotrexate

    Recent changes for Methotrexate

    Date
    Field
    Changed From
    Changed To
    1st February 2023
    Laboratory

    Referred Test

    Biochemistry

    Collection & Request Instructions

    Send ASAP (within 24 hrs, 7 days a week). Contact reference laboratory prior to sending the sample.

    Testing performed on an as needs basis. 

    External Laboratory

    Peter MacCallum Cancer Institute

    Method

    Hand deliover samples to Biochemistry DO NOT load onto the 8100


     Recent changes for Mumps Serology

    Recent changes for Mumps Serology

    Date
    Field
    Changed From
    Changed To
    18th January 2023
    Laboratory

    Microbiology

    Biochemistry

    Request Group

    SMUM

    MUM

    Frequency

    Daily

    Twice weekly


     Recent changes for Muscle Biopsy

    Recent changes for Muscle Biopsy

    Date
    Field
    Changed From
    Changed To
    30th December 2022
    Collection & Request Instructions

    Note: This is an URGENT send out test.

    Laboratory hours are 9am - 3pm, Monday - Friday.

     

    Note: This is an URGENT send out test.

    Laboratory hours are 9am - 3pm, Monday - Friday.

    Specimen must arrive at Alfred Pathology by 4pm.

     

    External Transport Instructions

    Place the specimen in an esky with ice cubes and dispatch immediately.

    Place the specimen in an esky with ice cubes and dispatch immediately. Must arrive at Alfred by 4pm.

    30th December 2022
    Collection & Request Instructions

    Note: This is an URGENT send out test.

    Laboratory hours are 9am - 5pm, Monday - Friday.

     

    Note: This is an URGENT send out test.

    Laboratory hours are 9am - 3pm, Monday - Friday.

     


     Recent changes for Paroxysmal Nocturnal Haemoglobinuria

    Recent changes for Paroxysmal Nocturnal Haemoglobinuria

    Date
    Field
    Changed From
    Changed To
    15th October 2021
    Collection & Request Instructions

    Separate specimen required.

    Cutoff for outpatient sample collection on Fridays is at 11.30 AM and MUST reach the laboratory before 4.00PM.

    Whole blood - 1 x EDTA sample, do not spin. Store at room temperature. An FBE and BLOOD FILM must also be requested,  and needs a separate EDTA sample.

    Cutoff for outpatient sample collection on Fridays is at 11.30 AM and MUST reach the laboratory before 4.00PM.

    12th October 2021
    Alternative names

    CD 59, PNH flow cytometry, PNH screen

    CD59, PNH screen flow cytometry, FLEAR

    12th October 2021
    Alternative names

    CD 59, PNH screen

    CD 59, PNH screen, PNH flow cytometry

    Request Group

    FLOW

    PNHS

    20th July 2021
    Collection & Request Instructions

    Separate specimen required.

    Separate specimen required.

    Cutoff for outpatient sample collection on Fridays is at 11.30 AM and MUST reach the laboratory before 4.00PM.


     Recent changes for Procalcitonin

    Recent changes for Procalcitonin

    Date
    Field
    Changed From
    Changed To
    17th November 2022
    Method

    All PCT requests must be discussed with the Chemical Pathologist upon receipt in lab, regardless of time of day or day of week. If urgent/Taxi requiring is determined, result must be phoned to the clinician upon receipt - High Risk Result.

    17th August 2022
    Method

    All PCT requests must be discussed with the Chemical Pathologist upon receipt in lab, regardless of time of day or day of week. If urgent/Taxi requiring is determined, result must be phoned to the clinician upon receipt - High Risk Result.

    27th July 2022
    Laboratory

    Biochemistry

    Referred Test

    Volume (Adults)

    0.5 mL

    External Laboratory

    Royal Childrens Hospital


     Recent changes for Prothrombin Time

    Recent changes for Prothrombin Time

    Date
    Field
    Changed From
    Changed To
    25th March 2022
    Collection & Request Instructions

    Filled to label marker and mixed by inversion.

    Patient Information - Warfarin Management

     

    Filled to label marker and mixed by inversion.

    Patient Information - Warfarin Management-English
    Patient Information - Warfarin Management-Greek
    Patient Information - Warfarin Management-Simplified Chinese

     

     


     Recent changes for Q Fever Serology

    Recent changes for Q Fever Serology

    Date
    Field
    Changed From
    Changed To
    8th February 2023
    External Laboratory

    Victorian Infectious Diseases Reference Laboratory (V.I.D.R.L)


     Recent changes for Red Cell Antigen Genotyping

    Recent changes for Red Cell Antigen Genotyping

    Date
    Field
    Changed From
    Changed To
    9th May 2022
    Collection & Request Instructions

    Minimum volume of 4 ml required - this specimen cannot be used for any other purpose.

    A completed "ARCBS Red Cell Reference Form" must accompany each sample sent for analysis.

    This form, which can be completed online and subsequently printed to be sent with the sample can be found by clicking here 

    Minimum volume of 4 ml required - this specimen cannot be used for any other purpose.

    A completed "ARCBS Red Cell Reference Form" must accompany each sample sent for analysis.

    This form, which can be completed online and subsequently printed to be sent with the sample can be found by clicking here 

    Processing Instructions

    Specimen will arrive in department at RT.  Please keep at 4 degrees prior to transport.

    Specimen will arrive in department at RT.  Please keep at 4 degrees prior to transport.

    External Transport Instructions

    Transport at 4 degrees

    Transport at 4 degrees


     Recent changes for Respiratory Virus PCR

    Recent changes for Respiratory Virus PCR

    Date
    Field
    Changed From
    Changed To
    14th February 2023
    Method

    The following specimens will be referred for testing-

    Sputum, Bronchial Washings, Bronchoalveolar Lavages - VIDRL

    Swab specimens for Respiratory Multiplex PCR will be referred to Monash Molecular Laboratory

     

    The following specimens will be referred for testing-

    Sputum, Bronchial Washings, Bronchoalveolar Lavages - VIDRL

    As of Feb 2023 swab specimens for Respiratory Multiplex PCR will be tested in-house.

     

    31st August 2022
    Preferred Specimen Type

    Nasopharyngeal swab
    Nasopharyngeal Aspirate

    Nasopharyngeal swab
    NTS - nose & throat swab
    Nasopharyngeal Aspirate

    Optional Specimen Type

    Sputum
    Bronchial Washings
    Broncho-alveolar Lavage

    Preferred Container Type

    Viral Transport Media- Nasopharyngeal
    Respiratory aspiration pot

    Viral Transport Media- Nasopharyngeal
    Respiratory aspiration pot
    respiratory PCR requirements

    Collection & Request Instructions

    Please note these swabs are available from stores.

    Search for: VIRAL TRANSPORT MEDIA - NASOPHARYNGEAL

    Pathology do not keep a stock of these swabs. Emergency departments and wards should manage a supply of these swabs according to demand.

     

     

    Please note these swabs are available from stores.

    Pathology do not keep a stock of these swabs. Emergency departments and wards should manage a supply of these swabs according to demand.

     

     

    Method

    All tests for respiratory virus PCR are considered urgent and must be delivered to the laboratory ASAP.

    ICU/Oncology patients testing negative for Flu A/B and RSV  will be referred to Monash for respiratory virus panel. This includes Influenza A virus, Influenza B virus, Respiratory Syncitial Virus, Picornavirus (RV/EV), Parainfluenza viruses 1,2 and 3, Adenovirus, Human Metapneumovirus, Bordetella pertussis, Bordetella parapertussis, Mycoplasma pneumoniae, Legionella species, Chlamydiaceae.

    The following specimens will be referred for testing-

    Sputum, Bronchial Washings, Bronchoalveolar Lavages

    Specimens for Respiratory Multiplex PCR will be referred to Monash Molecular Laboratory

    Contact Monash Pathology 9594 4532 or if no answer try Bacteriology 9594 4565 as there is someone rostered 24/7

     

    The following specimens will be referred for testing-

    Sputum, Bronchial Washings, Bronchoalveolar Lavages - VIDRL

    Swab specimens for Respiratory Multiplex PCR will be referred to Monash Molecular Laboratory

     

    Notes

    This PCR will detect and identify: Influenza A virus, Influenza B virus and Respiratory syncytial virus (RSV).

    Transport at room temperature to the Laboratory

    Testing is performed at Box Hill, Angliss, and Maroondah.

    Testing  Lab

    Testing hours

    TAT (from receipt in lab)

    All sites

     

    Available 24/7 

    1 hr

    Samples received after hours may have a longer TAT due to other urgent critical pathology testing occurring.

    This PCR will detect and identify: Influenza A virus, Influenza B virus and Respiratory syncytial virus (RSV).

    Transport at room temperature to the Laboratory

    Testing is performed at Box Hill, Angliss, and Maroondah.

    Testing  Lab

    Testing hours

    TAT (from receipt in lab)

    All sites

     

    Available 24/7 

    6-12 hrs routine

    2 hours (rapid approval form required)

    DO NOT send samples in the Pathology Chute

    For Rapid testing from Emergency Department - swab must be accompanied by signed Rapid Test Authorisation Form.

    DO NOT call the laboratory for results/enquiries. Inpatient tests will be prioritised and results available on EMR ASAP.

    Sputum samples are referred to an external laboratory - results will be delayed.


     Recent changes for Respiratory Virus PCR - Extended

    Recent changes for Respiratory Virus PCR - Extended

    Date
    Field
    Changed From
    Changed To
    14th February 2023
    Preferred Container Type

    Viral Transport Media - Nasopharyngeal

    Viral Transport Media - Nasopharyngeal
    respiratory PCR requirements
    Viral Transport Media- Nasopharyngeal

    14th February 2023
    Preferred Specimen Type

    NTS - nose & throat swab
    Swab

    NTS - nose & throat swab
    Respiratory samples


     Recent changes for Rickettsia spp. Screen Serology

    Recent changes for Rickettsia spp. Screen Serology

    Date
    Field
    Changed From
    Changed To
    8th February 2023
    External Laboratory

    Victorian Infectious Diseases Reference Laboratory (V.I.D.R.L)


     Recent changes for Ristocetin Co-Factor assay

    Recent changes for Ristocetin Co-Factor assay

    Date
    Field
    Changed From
    Changed To
    11th June 2021
    Alternative names

    RICOF

    RICOF, vWF:Activity, vWF:Act

    Laboratory

    Referred Test

    Coagulation

    External Laboratory

    Austin Pathology


     Recent changes for Scabies investigation

    Recent changes for Scabies investigation

    Date
    Field
    Changed From
    Changed To
    8th June 2021
    Collection & Request Instructions

    Scabies collection instructions

    Preparation

    Wash the area of skin to be scraped with an alcohol swab.

    Remove any ointment or residual applications using soap and water.

    Disinfect the area with a skin cleansing alcohol wipe and allow it to dry.

    Abrade the infested area with a sterile scalpel to the depth of pinpoint bleeding.

    Transfer skin scrapings to a sterile yellow top pot for transport to the laboratory. Do NOT include the scalpel – dispose of in sharps bin

    Label pot with patient name, UR No and DOB, date of collection, site of collection

    Preparation

    Wash the area of skin to be scraped with an alcohol swab.

    Remove any ointment or residual applications using soap and water.

    Disinfect the area with a skin cleansing alcohol wipe and allow it to dry.

    Abrade the infested area with a sterile scalpel to the depth of pinpoint bleeding.

    Transfer skin scrapings to a sterile yellow top pot for transport to the laboratory. Do NOT include the scalpel – dispose of in sharps bin

    Label pot with patient name, UR No and DOB, date of collection, site of collection


     Recent changes for Thiopurine Metabolites

    Recent changes for Thiopurine Metabolites

    Date
    Field
    Changed From
    Changed To
    3rd March 2023
    Preferred Container Type

    Lithium heparin no gel
    EDTA

    See Instructions

    25th August 2022
    Collection & Request Instructions

    Collect 1 x 4 ml Lithium Heparin tube and

    1 x 4ml EDTA tube.

    FBE must be requested with this test as a RCC is required for calculation of results

    DO NOT SPIN.

    Keep at Room Temp.

     

    Collect 1 x 4 ml Lithium Heparin tube and

    2 x 4ml EDTA tube.

    FBE must be requested with this test as a RCC is required for calculation of results

    DO NOT SPIN.

    Keep at Room Temp.

     

    23rd August 2022
    Collection & Request Instructions

    Collect 1 x 4 ml Lithium Heparin tube and 1 x 4ml EDTA tube.

    FBE must be requested with this test as a RCC is required for calculation of results

    DO NOT SPIN.

    Keep at Room Temp.

     

    Collect 1 x 4 ml Lithium Heparin tube and

    1 x 4ml EDTA tube.

    FBE must be requested with this test as a RCC is required for calculation of results

    DO NOT SPIN.

    Keep at Room Temp.

     

    23rd August 2022
    Request Group

    TMETAB

    Preferred Container Type

    EDTA

    Lithium heparin no gel
    EDTA

    Collection & Request Instructions

    Transport specimen at Room Temperature to lab

    Collection & Request Instructions

    Collect 2x4ml EDTA blood. DO NOT SPIN

    Keep at 4 deg C, do not freeze.

    The metabolites are stable for upto 4 days at 4 degress.

    Collect 1 x 4 ml Lithium Heparin tube and 1 x 4ml EDTA tube.

    FBE must be requested with this test as a RCC is required for calculation of results

    DO NOT SPIN.

    Keep at Room Temp.

     

    Processing Instructions

    Keep samples at Room Temperature.

    Do Not Spin. Whole Blood required

    External Laboratory

    St Vincents Hospital

    Frequency

    Weekly

    Notes

    Imuran is a trade name for azathioprine

    The Thiopurine metabolites (6-TGN & 6-MMP) are used as a means of estimating the toxicity and efficacy of azathioprine treatment.

    This test is performed by the Gastroenerology department. ( Phone No.9895 4632 )

    The cost for the test is not covered by Medicare

    Eastern Health patients:6TGN 6MMP Thiopurine Metabolites Testing $75

    All other patients: 6TGN 6MMP Thiopurine Metabolites Testing $145

    Imuran is a trade name for azathioprine

    The Thiopurine metabolites (6-TGN & 6-MMP) are used as a means of estimating the toxicity and efficacy of azathioprine treatment.

     

     


     Recent changes for Tissue release from Anatomical Pathology

    Recent changes for Tissue release from Anatomical Pathology

    Date
    Field
    Changed From
    Changed To
    6th March 2022
    Laboratory

    Unassigned

    Anatomical Pathology

    6th March 2022
    External Transport Instructions

    https://pathology.easternhealth.org.au/handbook/downloads/anatomical-pathology/Tissue%20Release%20Consent%20Form.doc


     Recent changes for Toxoplasma serology

    Recent changes for Toxoplasma serology

    Date
    Field
    Changed From
    Changed To
    18th January 2023
    Request Group

    STOX

    TOX


     Recent changes for Transthyretin

    Recent changes for Transthyretin

    Date
    Field
    Changed From
    Changed To
    13th September 2022
    Processing Instructions

    The serum must be aliquoted and frozen. Transthyretin is only stable for 3 days at 4-8C .

    The serum must be aliquoted and frozen. Transthyretin is only stable for 3 days at 4-8C .

    External Laboratory

    Monash Pathology


     Recent changes for Typhoid

    Recent changes for Typhoid

    Date
    Field
    Changed From
    Changed To
    31st January 2023
    Alternative names

    enteric fever, Typhoid fever

    enteric fever, typhoid fever

    31st January 2023
    Preferred Container Type

    Blood Culture

    Blood Culture (Adult)
    Blood Culture (Paediatric)


     Recent changes for Vitamin B1

    Recent changes for Vitamin B1

    Date
    Field
    Changed From
    Changed To
    18th November 2022
    External Laboratory

    Australian Clinical Labs (formerly Healthscope)

    St Vincents Hospital


     Recent changes for Vitamin B6

    Recent changes for Vitamin B6

    Date
    Field
    Changed From
    Changed To
    18th November 2022
    External Laboratory

    Australian Clinical Labs (formerly Healthscope)

    St Vincents Hospital


     Recent changes for Vitamin C

    Recent changes for Vitamin C

    Date
    Field
    Changed From
    Changed To
    31st March 2022
    Volume (Adults)

    2 mL

    1 mL

    Minimum/Paediatric Volume

    1 mL

    Processing Instructions

    Spin, separate, wrap in foil and freeze ASAP.

    Transport frozen. Contact Gribbles to arrange courier.

    Spin, separate, wrap in foil and freeze ASAP.

    Transport frozen. 

    External Laboratory

    Australian Clinical Labs (formerly Healthscope)

    Alfred Hospital

    Frequency

    Weekly

    Fortnightly


     Recent changes for Xanthochromia

    Recent changes for Xanthochromia

    Date
    Field
    Changed From
    Changed To
    14th July 2021
    Laboratory

    Referred Test

    Biochemistry

    Collection & Request Instructions

    Please note : to avoid sample deterioration, DO NOT send CSF specimens via the vacuum chute system.

    Please quickly check that the tube is labelled underneath the foil before referring.

    THERE MUST BE A MINIMUM OF 0.6 mL OF CSF OR THE TEST CANNOT BE PERFORMED

     

    Please note : to avoid sample deterioration, DO NOT send CSF specimens via the vacuum chute system.

     

    THERE MUST BE A MINIMUM OF 0.6 mL OF CSF OR THE TEST CANNOT BE PERFORMED

     

    Processing Instructions

    CSF xanthochromia checklist

    Sample collected >12hrs from onset symptoms   Preferable

     

          4th CSF sample    Essential

     

    Sample light protected immediately   Essential

     

    Sample transported manually to lab   Essential

     

    Serum sample for bilirubin & albumin   Preferable

     

    Slip with clinical notes and contact details   Essential

     

    CSF xanthochromia checklist

    Sample collected >12hrs from onset symptoms   Preferable

     

          4th CSF sample    Essential

     

    Sample light protected immediately   Essential

     

    Sample transported manually to lab   Essential

     

    Serum sample for bilirubin & protein- essemtial

     

    Slip with clinical notes and contact details   Essential

     

    External Laboratory

    Austin Pathology

    External Transport Instructions

    Send via a Taxi ASAP. Ring Austin Pathology on 94963100.

    MH and AH send via taxi ASAP and notify BH laboraotry.

    Frequency

    Daily

    As required

    Method

    Please quickly check that the tube is labelled underneath the foil before passing to Scientist.

    The specimen designated for spectrophotometry should be centrifuged at .2000 rpm for 5 min as soon as possible after receipt in the laboratory. The supernatant must be stored in the dark at 40 C until analysis.

    Notes

    This test is specialised and will only be performed on CSF's when:

    CT scan is negative

     

    Four tubes must be collected and the container of the 4th tube must be wrapped in foil (please Label specimen before wrapping in foil)

    This test is specialised and will only be performed on CSF's when:

    CT scan is negative

    Four tubes must be collected and the container of the 4th tube must be wrapped in foil (please Label specimen before wrapping in foil)

    A white top tube must be collected for simulataneous measurement of serum Bilirubin and serum Protein (or add on to existing bloods within 6 hours of CSF collection).