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Recent changes for Adrenocorticotrophic Hormone
Recent changes for Adrenocorticotrophic Hormone |
Date | Field | Changed From | Changed To |
2nd August 2022 | Notes | Consultation with Endocrinologist suggested
Collect in the morning between 0800 and 1000h.
1 x 4mL EDTA tube on ice.
Send to laboratory for immediate centrifugation. Spin, separate and freeze plasma. Transport frozen.
| Consultation with Endocrinologist suggested
Collect 1 x 4mL EDTA tube on ice.
Send to laboratory for immediate centrifugation. Spin, separate and freeze plasma.
|
Recent changes for Anti-diuretic hormone
Recent changes for Anti-diuretic hormone |
Date | Field | Changed From | Changed To |
7th October 2021 | Alternative names | ADH | |
| Request Group | ADH | |
| Preferred Specimen Type | Plasma | Serum |
| Optional Specimen Type | | Plasma |
| Preferred Container Type | EDTA | Serum tube with gel |
| Optional Container Type | | EDTA Lithium heparin no gel |
| Collection & Request Instructions | | Samples should be fasting. |
| Processing Instructions |
Spin, separate and freeze plasma.
Packed by certified personnel.
Sent frozen.
Specimen Reception, Level 6 (E Block), RPA, Missenden Road, Camperdown , NSW 2050
Tel: 02-95158279 | Spin, separate and freeze serum/plasma
Packed by certified personnel.
Sent frozen.
Specimen Reception, Level 6 (E Block), RPA, Missenden Road, Camperdown , NSW 2050
Tel: 02-95158279 |
Recent changes for Anti-Thrombin III
Recent changes for Anti-Thrombin III |
Date | Field | Changed From | Changed To |
19th January 2021 | Collection & Request Instructions | Fill to label marker and mix by inversion.
DO NOT use 1 mL citrate tubes.
1 x 3.5 ml citrate tubes sufficient for Protein C, Protein S and Antithrombin 3. | Fill to label marker and mix by inversion.
DO NOT use 1 mL citrate tubes.
Samples must be transported to laboratory immediately. ( Within a maximun of 4 hours from collection time)
1 x 3.5 ml citrate tubes sufficient for Protein C, Protein S and Antithrombin 3. |
10th December 2020 | Collection & Request Instructions | Fill to label marker and mix by inversion.
DO NOT use 1 mL citrate tubes. | Fill to label marker and mix by inversion.
DO NOT use 1 mL citrate tubes.
1 x 3.5 ml citrate tubes sufficient for Protein C, Protein S and Antithrombin 3. |
Recent changes for Anti-Xa Assay
Recent changes for Anti-Xa Assay |
Date | Field | Changed From | Changed To |
18th May 2021 | Request Group | 10A: Clexane, 10AH:Plasma Heparin, APIX: Apixaban, RIVX: Rivaroxaban | 10A: Clexane, 10AH:Plasma Heparin, APIX: Apixaban, RIVX: Rivaroxaban, FONX: Fondaparinux |
18th May 2021 | Request Group | 10A | 10A: Clexane, 10AH:Plasma Heparin, APIX: Apixaban, RIVX: Rivaroxaban |
Recent changes for Bile Acids
Recent changes for Bile Acids |
Date | Field | Changed From | Changed To |
23rd May 2022 | Laboratory | Referred Test | Biochemistry |
| Preferred Container Type | Plain Tube with gel | Serum tube with gel |
| Collection & Request Instructions | Fasting or Non Fasting sample.
Note: This test is ONLY performed on pregnant women.
| Fasting.
Note: This test is ONLY performed on pregnant women.
|
| Processing Instructions | Spin, separate ASAP. | |
| External Laboratory | Royal Childrens Hospital | |
| Frequency | Weekly | Twice weekly |
| Method | Store and transport at 4 C. | |
Recent changes for Blood Cultures - Adult
Recent changes for Blood Cultures - Adult |
Date | Field | Changed From | Changed To |
9th August 2021 | Processing Instructions | Bottles should be loaded on to the Bactec analyser as soon as possible. If entry is delayed, store bottles at room temperature. | Bottles should be loaded on to the Bactec FX analyser as soon as possible. If entry is delayed, store bottles at room temperature. |
9th August 2021 | Processing Instructions | Bottles should be loaded on to the Virtuo analyser as soon as possible. If entry is delayed, store bottles at room temperature. | Bottles should be loaded on to the Bactec analyser as soon as possible. If entry is delayed, store bottles at room temperature. |
25th May 2021 | Volume (Adults) | 10 mL | 8 mL |
| Preferred Container Type | Blood Culture | Blood Culture (Adult) |
Recent changes for Blood Cultures - Children and Neonates
Recent changes for Blood Cultures - Children and Neonates |
Date | Field | Changed From | Changed To |
9th August 2021 | External Transport Instructions | Load as soon as possible onto the Virtuo Analyzer. Store at room temperature until loaded | Load as soon as possible onto the Bactec FX Analyzer. Store at room temperature until loaded |
25th May 2021 | Volume (Adults) | 4 mL | |
| Minimum/Paediatric Volume | | 1 mL |
| Preferred Container Type | Blood Culture Paediatric | Blood Culture (Paediatric) |
| Collection & Request Instructions | Add 0.5 - 4mL of blood to a single aerobic paediatric bottle.
For patients with suspected bacterial endocarditis, 2 to 3 sets taken at different times should be sufficient to establish a diagnosis.
Collect blood cultures 20 minutes apart. If patient is too ill to wait 20 minutes, prior to administering antibiotics, collect the first set from one site and the second from a different site.
Please provide clinical details. Extended incubation is required for query endocarditis, brucellosis or fungal infection
Transport at room temperature
| Add 1 - 3mL of blood to a single aerobic paediatric bottle.
For patients with suspected bacterial endocarditis, 2 to 3 sets taken at different times should be sufficient to establish a diagnosis.
If multiple sets required, collect blood cultures 20 minutes apart. If patient is too ill to wait 20 minutes, prior to administering antibiotics, collect the first set from one site and the second from a different site.
Please provide clinical details. Extended incubation is required for query endocarditis, brucellosis or fungal infection
Transport at room temperature
|
Recent changes for Bone Marrow Examination
Recent changes for Bone Marrow Examination |
Date | Field | Changed From | Changed To |
25th March 2022 | Laboratory | Unassigned | Haematology |
Recent changes for Bone Specific Alkaline Phosphatase
Recent changes for Bone Specific Alkaline Phosphatase |
Date | Field | Changed From | Changed To |
15th June 2021 | Assay name | Bone Specific Alkiline Phosphatase | Bone Specific Alkaline Phosphatase |
| External Laboratory | Monash Pathology | Royal Melbourne Hospital |
Recent changes for BRCA1/BRCA2
Recent changes for BRCA1/BRCA2 |
Date | Field | Changed From | Changed To |
17th June 2021 | Request Group | | CHROM |
| Collection & Request Instructions | | Send to Peter Mac on next available courier. Stable at room temperature for a maximum of 2 weeks. |
| Processing Instructions | | Send to Peter Mac on next available courier. Stable at room temperature for a maximum of 2 weeks. |
| External Transport Instructions | Room Temperature | Room Temperature
Send to Peter Mac on next available courier. Stable at room temperature for a maximum of 2 weeks. |
| Method | | Send to Peter Mac on next available courier. Stable at room temperature for a maximum of 2 weeks. |
Recent changes for CASA
Recent changes for CASA |
Date | Field | Changed From | Changed To |
17th February 2022 | External Laboratory | Royal Brisbane & Womens Hospital | |
| External Transport Instructions | Transport frozen.
Packed by certified personnel. | Transport frozen.
Packed by certified personnel. |
| Notes | In combination with the tumour marker CA-125, CASA is used for differentiating between benign gynaecological disease and ovarian carcinoma. Please note that this test has changed referral laboratories as at 18/1/2011.
Please contact Chemical Pathologist before requesting this test. | In combination with the tumour marker CA-125, CASA is used for differentiating between benign gynaecological disease and ovarian carcinoma. Please note that this test has changed referral laboratories as at 18/1/2011. This test is no longer available in Australia
Please contact Chemical Pathologist before requesting this test.
|
Recent changes for Cerebrospinal Fluid - Biochemistry
Recent changes for Cerebrospinal Fluid - Biochemistry |
Date | Field | Changed From | Changed To |
27th April 2021 | Collection & Request Instructions | Please note : to avoid sample deterioration, DO NOT send CSF specimens via the vacuum chute system.
Depending on the tests requested, 3-4 separate tubes will need to be provided. Note that the tubes are numbered so that order of collection can be identified – this order should be carefully followed, so that the optimal sample can be used for the requested tests. It is also important to consider the tests that are required, as this will determine how much CSF is needed in each tube. See the matrix below for details.
TUBE 1 is used for Cytology.
TUBE 2 is used for Glucose, Protein and Flow Cytometry.
(centrifuged in the lab – the supernatant is used for Biochemistry analysis (glucose, protein) and the cellular pellet will be used for flow cytometry if required).
TUBE 3 is for Microbiological analysis (cell count, Gram stain and culture) and any further infectious testing (e.g. viral PCR, mycobacterial PCR and culture, cryptococcal antigen).
TUBE 4 will be required only for;
(a) Creutzfeld-Jacob Disease (CJD) testing OR
(b) Xanthochromia analysis (wrap the tube in foil to prevent sample deterioration due to light).
TESTS REQUIRED
|
MINIMUM REQUIRED VOLUME (mL)
|
TUBE 1
|
TUBE 2
|
TUBE 3
|
TUBE 4
|
CYTOLOGY. CHEMISTRY, MICRO/CULTURE
|
1.0-2.0
|
1.0
|
1.0-2.0
|
Nil
|
ADD FLOW CYTOMETRY
|
|
add 5.0-7.0
|
|
|
ADD OTHER INFECTIOUS MARKERS
|
|
|
add 2.0- 5.0-
|
|
ADD MYCOBACTERIAL CULTURE
|
|
|
Add 10.00
|
|
ADD CJD
|
|
|
|
1.0
|
ADD XANTHOCHROMIA
|
|
|
|
1.0 (foil)
|
Note, the ability to add further tests to a previously collected sample is dependent upon the volume provided. Also, many tests (flow cytometry, cytology) can only be performed on fresh specimens.
Other specialised testing (e.g. oligoclonal bands) will be performed using the supplied samples.
Xanthochromia and CJD analysis cannot be performed on a blood-stained sample.
CSF Information - Laboratory Processing | Depending on the tests requested, 3-4 separate tubes will need to be provided. Note that the tubes are numbered so that order of collection can be identified – this order should be carefully followed, so that the optimal sample can be used for the requested tests. It is also important to consider the tests that are required, as this will determine how much CSF is needed in each tube. See the matrix below for details.
TUBE 1 is used for Biochemistry and Flow Cytometry. (Centrifuged in the lab – the supernatant is used for Biochemistry analysis (glucose, protein) and the cellular pellet will be used for flow cytometry if required). If tube 3 is bloodstained, take an aliquot for microbiology.
TUBE 2 is used for Cytology
TUBE 3 is for Microbiological analysis (cell count, Gram stain and culture) and any further infectious testing (e.g. viral PCR, mycobacterial PCR and culture, cryptococcal antigen).
TUBE 4 will be required only for;
(a) Creutzfeld-Jacob Disease (CJD) testing OR
(b) Xanthochromia analysis (wrap the tube in foil to prevent sample deterioration due to light).
TESTS REQUIRED
|
MINIMUM REQUIRED VOLUME (mL)
|
TUBE 1
|
TUBE 2
|
TUBE 3
|
TUBE 4
|
CHEMISTRY
|
1.0
|
|
|
|
ADD FLOW CYTOMETRY
|
Minimum 5.0-7.0
|
|
|
|
CYTOLOGY
|
|
1.0-3.0
|
|
|
MICROBIOLOGY
|
|
|
1.0-2.0
|
|
ADD OTHER INFECTIOUS MARKERS
|
|
|
add 2.0- 5.0-
|
|
ADD MYCOBACTERIAL CULTURE
|
|
|
Add 6.0-10.00
|
|
ADD CJD
|
|
|
|
1.0
|
ADD XANTHOCHROMIA
|
|
|
|
1.0 (foil)
|
Note, the ability to add further tests to a previously collected sample is dependent upon the volume provided. Also, many tests (flow cytometry, cytology) can only be performed on fresh specimens.
Other specialised testing (e.g. oligoclonal bands) will be performed using the supplied samples.
Xanthochromia and CJD analysis cannot be performed on a blood-stained sample.
|
Recent changes for Cerebrospinal Fluid - Cytology
Recent changes for Cerebrospinal Fluid - Cytology |
Date | Field | Changed From | Changed To |
27th April 2021 | Collection & Request Instructions | The sample with the least amount of blood is prefered for Cytology.
CSF SPECIMEN INFORMATION – LABORATORY PROCESSING
Depending on the tests requested, 3-4 separate tubes will need to be provided. Note that the tubes are numbered so that order of collection can be identified – this order should be carefully followed, so that the optimal sample can be used for the requested tests. It is also important to consider the tests that are required, as this will determine how much CSF is needed in each tube. See the matrix below for details.
TUBE 1 is used for Cytology.
TUBE 2 is used for Glucose, Protein and Flow Cytometry.
(centrifuged in the lab – the supernatant is used for Biochemistry analysis (glucose, protein) and the cellular pellet will be used for flow cytometry if required).
TUBE 3 is for Microbiological analysis (cell count, Gram stain and culture) and any further infectious testing (e.g. viral PCR, mycobacterial PCR and culture, cryptococcal antigen).
TUBE 4 will be required only for;
(a) Creutzfeld-Jacob Disease (CJD) testing OR
(b) Xanthochromia analysis (wrap the tube in foil to prevent sample deterioration due to light).
TESTS REQUIRED
|
MINIMUM REQUIRED VOLUME (mL)
|
TUBE 1
|
TUBE 2
|
TUBE 3
|
TUBE 4
|
CYTOLOGY. CHEMISTRY, MICRO/CULTURE
|
1.0-2.0
|
1.0
|
1.0-2.0
|
Nil
|
ADD FLOW CYTOMETRY
|
|
add 5.0-7.0
|
|
|
ADD OTHER INFECTIOUS MARKERS
|
|
|
add 2.0- 5.0-
|
|
ADD MYCOBACTERIAL CULTURE
|
|
|
Add 10.00
|
|
ADD CJD
|
|
|
|
1.0
|
ADD XANTHOCHROMIA
|
|
|
|
1.0 (foil)
|
Note, the ability to add further tests to a previously collected sample is dependent upon the volume provided. Also, many tests (flow cytometry, cytology) can only be performed on fresh specimens.
Other specialised testing (e.g. oligoclonal bands) will be performed using the supplied samples.
Xanthochromia and CJD analysis cannot be performed on a blood-stained sample. | The sample with the least amount of blood is prefered for Cytology.
CSF SPECIMEN INFORMATION – LABORATORY PROCESSING
Depending on the tests requested, 3-4 separate tubes will need to be provided. Note that the tubes are numbered so that order of collection can be identified – this order should be carefully followed, so that the optimal sample can be used for the requested tests. It is also important to consider the tests that are required, as this will determine how much CSF is needed in each tube. See the matrix below for details.
TUBE 1 is used for Biochemistry and Flow Cytometry. (Centrifuged in the lab – the supernatant is used for Biochemistry analysis (glucose, protein) and the cellular pellet will be used for flow cytometry if required). If tube 3 is bloodstained, take an aliquot for microbiology.
TUBE 2 is used for Cytology
TUBE 3 is for Microbiological analysis (cell count, Gram stain and culture) and any further infectious testing (e.g. viral PCR, mycobacterial PCR and culture, cryptococcal antigen).
TUBE 4 will be required only for;
(a) Creutzfeld-Jacob Disease (CJD) testing OR
(b) Xanthochromia analysis (wrap the tube in foil to prevent sample deterioration due to light).
TESTS REQUIRED
|
MINIMUM REQUIRED VOLUME (mL)
|
TUBE 1
|
TUBE 2
|
TUBE 3
|
TUBE 4
|
CHEMISTRY
|
1.0
|
|
|
|
ADD FLOW CYTOMETRY
|
Minimum 5.0-7.0
|
|
|
|
CYTOLOGY
|
|
1.0-3.0
|
|
|
MICROBIOLOGY
|
|
|
1.0-2.0
|
|
ADD OTHER INFECTIOUS MARKERS
|
|
|
add 2.0- 5.0-
|
|
ADD MYCOBACTERIAL CULTURE
|
|
|
Add 6.0-10.00
|
|
ADD CJD
|
|
|
|
1.0
|
ADD XANTHOCHROMIA
|
|
|
|
1.0 (foil)
|
Note, the ability to add further tests to a previously collected sample is dependent upon the volume provided. Also, many tests (flow cytometry, cytology) can only be performed on fresh specimens.
Other specialised testing (e.g. oligoclonal bands) will be performed using the supplied samples.
Xanthochromia and CJD analysis cannot be performed on a blood-stained sample.
|
| Method | CSF sample processing:
If Cytology/Flow Cytometry requested : Tube 1-Biochemistry, Tube 2- Cytology/Flow Cytometry, Tube 3-Microbiology
If Cytology/Flow Cytometry not requested: Tube 1 and 3-Microbiology, Tube 2-Biochemistry
Any confusion between the terms cell count and cytology could be cleared up when the cell count is rung through to the requesting doctor by the scientist. At this point it could be determined whether cytology is a genuine request or not (all to be documented in F8 notes).
| |
Recent changes for Cerebrospinal Fluid - microscopy and culture
Recent changes for Cerebrospinal Fluid - microscopy and culture |
Date | Field | Changed From | Changed To |
27th April 2021 | Notes | CSF SPECIMEN INFORMATION – LABORATORY PROCESSING
Depending on the tests requested, 3-4 separate tubes will need to be provided. Note that the tubes are numbered so that order of collection can be identified – this order should be carefully followed, so that the optimal sample can be used for the requested tests. It is also important to consider the tests that are required, as this will determine how much CSF is needed in each tube. See the matrix below for details.
TUBE 1 is used for Cytology.
TUBE 2 is used for Glucose, Protein and Flow Cytometry.
(centrifuged in the lab – the supernatant is used for Biochemistry analysis (glucose, protein) and the cellular pellet will be used for flow cytometry if required).
TUBE 3 is for Microbiological analysis (cell count, Gram stain and culture) and any further infectious testing (e.g. viral PCR, mycobacterial PCR and culture, cryptococcal antigen).
TUBE 4 will be required only for;
(a) Creutzfeld-Jacob Disease (CJD) testing OR
(b) Xanthochromia analysis (wrap the tube in foil to prevent sample deterioration due to light).
TESTS REQUIRED
|
MINIMUM REQUIRED VOLUME (mL)
|
TUBE 1
|
TUBE 2
|
TUBE 3
|
TUBE 4
|
CYTOLOGY. CHEMISTRY, MICRO/CULTURE
|
1.0-2.0
|
1.0
|
1.0-2.0
|
Nil
|
ADD FLOW CYTOMETRY
|
|
add 5.0-7.0
|
|
|
ADD OTHER INFECTIOUS MARKERS
|
|
|
add 2.0- 5.0-
|
|
ADD MYCOBACTERIAL CULTURE
|
|
|
Add 10.00
|
|
ADD CJD
|
|
|
|
1.0
|
ADD XANTHOCHROMIA
|
|
|
|
1.0 (foil)
|
Note, the ability to add further tests to a previously collected sample is dependent upon the volume provided. Also, many tests (flow cytometry, cytology) can only be performed on fresh specimens.
Other specialised testing (e.g. oligoclonal bands) will be performed using the supplied samples.
Xanthochromia and CJD analysis cannot be performed on a blood-stained sample.
Special tests available on request:
PCR for viruses
Acid-fast stain and/or culture for Mycobacteria
Cryptococcal antigen
Serological test for Syphilis (must be paired with 5mL serum sample or test will not be performed)
Viral Serology, if CSF is accompanied by serum sample
Flow Cytometry - requires a 4th tube
The turnaround time for CSF microscopy are 30-40 minutes.
The turnaround time for CSF culture are 3-5 days. | Depending on the tests requested, 3-4 separate tubes will need to be provided. Note that the tubes are numbered so that order of collection can be identified – this order should be carefully followed, so that the optimal sample can be used for the requested tests. It is also important to consider the tests that are required, as this will determine how much CSF is needed in each tube. See the matrix below for details.
TUBE 1 is used for Biochemistry and Flow Cytometry. (Centrifuged in the lab – the supernatant is used for Biochemistry analysis (glucose, protein) and the cellular pellet will be used for flow cytometry if required). If tube 3 is bloodstained, take an aliquot for microbiology.
TUBE 2 is used for Cytology
TUBE 3 is for Microbiological analysis (cell count, Gram stain and culture) and any further infectious testing (e.g. viral PCR, mycobacterial PCR and culture, cryptococcal antigen).
TUBE 4 will be required only for;
(a) Creutzfeld-Jacob Disease (CJD) testing OR
(b) Xanthochromia analysis (wrap the tube in foil to prevent sample deterioration due to light).
TESTS REQUIRED
|
MINIMUM REQUIRED VOLUME (mL)
|
TUBE 1
|
TUBE 2
|
TUBE 3
|
TUBE 4
|
CHEMISTRY
|
1.0
|
|
|
|
ADD FLOW CYTOMETRY
|
Minimum 5.0-7.0
|
|
|
|
CYTOLOGY
|
|
1.0-3.0
|
|
|
MICROBIOLOGY
|
|
|
1.0-2.0
|
|
ADD OTHER INFECTIOUS MARKERS
|
|
|
add 2.0- 5.0-
|
|
ADD MYCOBACTERIAL CULTURE
|
|
|
Add 6.0-10.00
|
|
ADD CJD
|
|
|
|
1.0
|
ADD XANTHOCHROMIA
|
|
|
|
1.0 (foil)
|
Note, the ability to add further tests to a previously collected sample is dependent upon the volume provided. Also, many tests (flow cytometry, cytology) can only be performed on fresh specimens.
Other specialised testing (e.g. oligoclonal bands) will be performed using the supplied samples.
Xanthochromia and CJD analysis cannot be performed on a blood-stained sample. |
Recent changes for Cerebrospinal Fluid Specimen Collection
Recent changes for Cerebrospinal Fluid Specimen Collection |
Date | Field | Changed From | Changed To |
27th April 2021 | Notes | Depending on the tests requested, 3-4 separate tubes will need to be provided. Note that the tubes are numbered so that order of collection can be identified – this order should be carefully followed, so that the optimal sample can be used for the requested tests. It is also important to consider the tests that are required, as this will determine how much CSF is needed in each tube. See the matrix below for details.
TUBE 1 is used for Biochemistry and Flow Cytometry. (Centrifuged in the lab – the supernatant is used for Biochemistry analysis (glucose, protein) and the cellular pellet will be used for flow cytometry if required). If tube 3 is bloodstained, take an aliquot for microbiology.
TUBE 2 is used for Cytology
TUBE 3 is for Microbiological analysis (cell count, Gram stain and culture) and any further infectious testing (e.g. viral PCR, mycobacterial PCR and culture, cryptococcal antigen).
TUBE 4 will be required only for;
(a) Creutzfeld-Jacob Disease (CJD) testing OR
(b) Xanthochromia analysis (wrap the tube in foil to prevent sample deterioration due to light).
TESTS REQUIRED
|
MINIMUM REQUIRED VOLUME (mL)
|
TUBE 1
|
TUBE 2
|
TUBE 3
|
TUBE 4
|
CHEMISTRY
|
1.0
|
|
|
|
ADD FLOW CYTOMETRY
|
Minimum 5.0-7.0
|
|
|
|
CYTOLOGY
|
|
1.0-3.0
|
|
|
MICROBIOLOGY
|
|
|
1.0-2.0
|
|
ADD OTHER INFECTIOUS MARKERS
|
|
|
add 2.0- 5.0-
|
|
ADD MYCOBACTERIAL CULTURE
|
|
|
Add 6.0-10.00
|
|
ADD CJD
|
|
|
|
1.0
|
ADD XANTHOCHROMIA
|
|
|
|
1.0 (foil)
|
Note, the ability to add further tests to a previously collected sample is dependent upon the volume provided. Also, many tests (flow cytometry, cytology) can only be performed on fresh specimens.
Other specialised testing (e.g. oligoclonal bands) will be performed using the supplied samples.
Xanthochromia and CJD analysis cannot be performed on a blood-stained sample.
| Depending on the tests requested, 3-4 separate tubes will need to be provided. Note that the tubes are numbered so that order of collection can be identified – this order should be carefully followed, so that the optimal sample can be used for the requested tests. It is also important to consider the tests that are required, as this will determine how much CSF is needed in each tube. See the matrix below for details.
TUBE 1 is used for Biochemistry and Flow Cytometry. (Centrifuged in the lab – the supernatant is used for Biochemistry analysis (glucose, protein) and the cellular pellet will be used for flow cytometry if required). If tube 3 is bloodstained, take an aliquot for microbiology.
TUBE 2 is used for Cytology
TUBE 3 is for Microbiological analysis (cell count, Gram stain and culture) and any further infectious testing (e.g. viral PCR, mycobacterial PCR and culture, cryptococcal antigen).
TUBE 4 will be required only for;
(a) Creutzfeld-Jacob Disease (CJD) testing OR
(b) Xanthochromia analysis (wrap the tube in foil to prevent sample deterioration due to light).
TESTS REQUIRED
|
MINIMUM REQUIRED VOLUME (mL)
|
TUBE 1
|
TUBE 2
|
TUBE 3
|
TUBE 4
|
CHEMISTRY
|
1.0
|
|
|
|
ADD FLOW CYTOMETRY
|
Minimum 5.0-7.0
|
|
|
|
CYTOLOGY
|
|
1.0-3.0
|
|
|
MICROBIOLOGY
|
|
|
1.0-2.0
|
|
ADD OTHER INFECTIOUS MARKERS
|
|
|
add 2.0- 5.0-
|
|
ADD MYCOBACTERIAL CULTURE
|
|
|
Add 6.0-10.00
|
|
ADD CJD
|
|
|
|
1.0
|
ADD XANTHOCHROMIA
|
|
|
|
1.0 (foil)
|
Note, the ability to add further tests to a previously collected sample is dependent upon the volume provided. Also, many tests (flow cytometry, cytology) can only be performed on fresh specimens.
Other specialised testing (e.g. oligoclonal bands) will be performed using the supplied samples.
Xanthochromia and CJD analysis cannot be performed on a blood-stained sample.
|
27th April 2021 | Notes | CSF SPECIMEN INFORMATION – LABORATORY PROCESSING
Depending on the tests requested, 3-4 separate tubes will need to be provided. Note that the tubes are numbered so that order of collection can be identified – this order should be carefully followed, so that the optimal sample can be used for the requested tests. It is also important to consider the tests that are required, as this will determine how much CSF is needed in each tube. See the matrix below for details.
TUBE 1 is used for Cytology.
TUBE 2 is used for Glucose, Protein and Flow Cytometry.
(centrifuged in the lab – the supernatant is used for Biochemistry analysis (glucose, protein) and the cellular pellet will be used for flow cytometry if required).
TUBE 3 is for Microbiological analysis (cell count, Gram stain and culture) and any further infectious testing (e.g. viral PCR, mycobacterial PCR and culture, cryptococcal antigen).
TUBE 4 will be required only for;
(a) Creutzfeld-Jacob Disease (CJD) testing OR
(b) Xanthochromia analysis (wrap the tube in foil to prevent sample deterioration due to light).
TESTS REQUIRED
|
MINIMUM REQUIRED VOLUME (mL)
|
TUBE 1
|
TUBE 2
|
TUBE 3
|
TUBE 4
|
CYTOLOGY. CHEMISTRY, MICRO/CULTURE
|
1.0-2.0
|
1.0
|
1.0-2.0
|
Nil
|
ADD FLOW CYTOMETRY
|
|
add 5.0-7.0
|
|
|
ADD OTHER INFECTIOUS MARKERS
|
|
|
add 2.0- 5.0-
|
|
ADD MYCOBACTERIAL CULTURE
|
|
|
Add 10.00
|
|
ADD CJD
|
|
|
|
1.0
|
ADD XANTHOCHROMIA
|
|
|
|
1.0 (foil)
|
Note, the ability to add further tests to a previously collected sample is dependent upon the volume provided. Also, many tests (flow cytometry, cytology) can only be performed on fresh specimens.
Other specialised testing (e.g. oligoclonal bands) will be performed using the supplied samples.
Xanthochromia and CJD analysis cannot be performed on a blood-stained sample. | Depending on the tests requested, 3-4 separate tubes will need to be provided. Note that the tubes are numbered so that order of collection can be identified – this order should be carefully followed, so that the optimal sample can be used for the requested tests. It is also important to consider the tests that are required, as this will determine how much CSF is needed in each tube. See the matrix below for details.
TUBE 1 is used for Biochemistry and Flow Cytometry. (Centrifuged in the lab – the supernatant is used for Biochemistry analysis (glucose, protein) and the cellular pellet will be used for flow cytometry if required). If tube 3 is bloodstained, take an aliquot for microbiology.
TUBE 2 is used for Cytology
TUBE 3 is for Microbiological analysis (cell count, Gram stain and culture) and any further infectious testing (e.g. viral PCR, mycobacterial PCR and culture, cryptococcal antigen).
TUBE 4 will be required only for;
(a) Creutzfeld-Jacob Disease (CJD) testing OR
(b) Xanthochromia analysis (wrap the tube in foil to prevent sample deterioration due to light).
TESTS REQUIRED
|
MINIMUM REQUIRED VOLUME (mL)
|
TUBE 1
|
TUBE 2
|
TUBE 3
|
TUBE 4
|
CHEMISTRY
|
1.0
|
|
|
|
ADD FLOW CYTOMETRY
|
Minimum 5.0-7.0
|
|
|
|
CYTOLOGY
|
|
1.0-3.0
|
|
|
MICROBIOLOGY
|
|
|
1.0-2.0
|
|
ADD OTHER INFECTIOUS MARKERS
|
|
|
add 2.0- 5.0-
|
|
ADD MYCOBACTERIAL CULTURE
|
|
|
Add 6.0-10.00
|
|
ADD CJD
|
|
|
|
1.0
|
ADD XANTHOCHROMIA
|
|
|
|
1.0 (foil)
|
Note, the ability to add further tests to a previously collected sample is dependent upon the volume provided. Also, many tests (flow cytometry, cytology) can only be performed on fresh specimens.
Other specialised testing (e.g. oligoclonal bands) will be performed using the supplied samples.
Xanthochromia and CJD analysis cannot be performed on a blood-stained sample.
|
Recent changes for Coagulation Factor Assays II, V, VII, VIII, IX, X, XI, XII
Recent changes for Coagulation Factor Assays II, V, VII, VIII, IX, X, XI, XII |
Date | Field | Changed From | Changed To |
10th December 2020 | Collection & Request Instructions | Do not use 1.0 mL citrate tubes
Collect 1 X 3.5 ml Sodium Citrate tube is required to run all Factor assay ( Excent Factor XIII which is a refered test).
1 Tube is sufficient for performing these tests.
| Do not use 1.0 mL citrate tubes
Collect 1 X 3.5 ml Sodium Citrate tube is sufficient to run all Factor assay ( Excent Factor XIII which is a refered test).
1 Tube is sufficient for performing these tests.
|
10th December 2020 | Collection & Request Instructions | Do not use 1.0 mL citrate tubes | Do not use 1.0 mL citrate tubes
Collect 1 X 3.5 ml Sodium Citrate tube is required to run all Factor assay ( Excent Factor XIII which is a refered test).
1 Tube is sufficient for performing these tests.
|
Recent changes for Collagen Binding Assay
Recent changes for Collagen Binding Assay |
Date | Field | Changed From | Changed To |
11th June 2021 | Laboratory | Referred Test | Coagulation |
| Request Group | | CBA |
| Collection & Request Instructions |
Fill to label marker and mix by inversion.
Do not use 1 mL citrate tubes. | Fill to label marker and mix by inversion.
Do not use 1 mL citrate tubes. |
| Processing Instructions |
Specimens kept at room temperature, should be centrifuged ASAP and tested within 4 hours from time of specimen collection. If testing is not completed within 4 hours, plasma should be removed from cells and aliquoted (1mL) into Eppendorf tubes, 2 X 1 mL vials per CBA assay.
Plasma may be frozen @ -20 C for up to 2 weeks or -70 C for up to 6 months. Frost free freezers should not be used. | Specimens kept at room temperature, should be centrifuged ASAP and tested within 4 hours from time of specimen collection. If testing is not completed within 4 hours, plasma should be removed from cells and aliquoted (1mL) into Eppendorf tubes, 2 X 1 mL vials per CBA assay.
Plasma may be frozen @ -20 C for up to 2 weeks or -70 C for up to 6 months. Frost free freezers should not be used. |
| External Laboratory | Austin Pathology | |
| Frequency | | Fortnightly |
Recent changes for Copper-Liver
Recent changes for Copper-Liver |
Date | Field | Changed From | Changed To |
10th May 2021 | External Laboratory | Australian Clinical Labs (formerly Healthscope) | Pacific Laboratory Medicine Services |
Recent changes for COVID-19 Antibody
Recent changes for COVID-19 Antibody |
Date | Field | Changed From | Changed To |
24th September 2020 | Assay name | covab | COVAB |
| Alternative names | | SARS-CoV-2 Antibody
COVID Antibody
COVID Serology |
| Laboratory | Unassigned | Biochemistry |
| Request Group | | COVAB |
| Collection & Request Instructions | | Transport specimen at Room Temperature to lab |
| Frequency | | Daily |
Recent changes for COVID-19 PCR
Recent changes for COVID-19 PCR |
Date | Field | Changed From | Changed To |
16th September 2020 | External Laboratory | | Victorian Infectious Diseases Reference Laboratory (V.I.D.R.L) |
16th September 2020 | Collection & Request Instructions | Nasopharyngeal swab in RED Copan viral transport medium
Nasal/oropharyngeal swab in GREEN Kang Jian viral transport medium | Nasal/oropharyngeal swab in GREEN Kang Jian viral transport medium
Paediatric - Nasopharyngeal swab in RED Copan viral transport medium |
| Notes | For Rapid testing from Emergency Department - swab must be accompanied by signed Rapid Test Authorisation Form.
DO NOT call the laboratory for results/enquiries. Inpatient tests will be prioritised and results available on EMR ASAP.
Sputum samples are referred to an external laboratory - results will be delayed. | Samples MUST be in a clear specimen bag within an outer GREEN specimen bag
DO NOT send samples in the Pathology Chute
For Rapid testing from Emergency Department - swab must be accompanied by signed Rapid Test Authorisation Form.
DO NOT call the laboratory for results/enquiries. Inpatient tests will be prioritised and results available on EMR ASAP.
Sputum samples are referred to an external laboratory - results will be delayed. |
16th September 2020 | Preferred Specimen Type | Nasopharyngeal swab NTS - nose & throat swab | Nasopharyngeal swab NTS - nose & throat swab Sputum |
| Preferred Container Type | Viral Transport Media - Nasopharyngeal respiratory PCR requirements See Instructions | Viral Transport Media - Nasopharyngeal See Instructions Sterile Jar |
| Notes | For Rapid testing from Emergency Department - swab must be accompanied by signed Rapid Test Authorisation Form.
DO NOT call the laboratory for results/enquiries. Inpatient tests will be prioritised and results available on EMR. | For Rapid testing from Emergency Department - swab must be accompanied by signed Rapid Test Authorisation Form.
DO NOT call the laboratory for results/enquiries. Inpatient tests will be prioritised and results available on EMR ASAP.
Sputum samples are referred to an external laboratory - results will be delayed. |
Recent changes for DNA analysis for Thalassaemia (related to Hgb Electrophoresis)
Recent changes for DNA analysis for Thalassaemia (related to Hgb Electrophoresis) |
Date | Field | Changed From | Changed To |
10th December 2020 | Collection & Request Instructions | Send FBE, Iron studies and Hb electrophoresis results.
Send within 24 Hrs.
Results: 95943398 | Please collect 2x EDTA tube for this test.
Send FBE, Iron studies and Hb electrophoresis results.
Send within 24 Hrs.
Results: 95943398 |
Recent changes for Euglobulin Clot Lysis Time
Recent changes for Euglobulin Clot Lysis Time |
Date | Field | Changed From | Changed To |
10th December 2020 | Volume (Adults) | | |
| Method | | This test is no longer performed. Please contact Haematologist or Senior Scientist in Haematology if further information is required. |
Recent changes for Fluconazole
Recent changes for Fluconazole |
Date | Field | Changed From | Changed To |
14th October 2021 | Collection & Request Instructions |
|
|
| Processing Instructions |
Spin, separate Plasma and freeze.
Packed by certified personnel.
Results:02-83829100 or 02-82829190 | Spin and separate Plasma.
|
| External Laboratory | Sydpath Pathology, St Vincent's Hospital NSW | Alfred Hospital |
Recent changes for Free PSA
Recent changes for Free PSA |
Date | Field | Changed From | Changed To |
21st June 2021 | External Laboratory | Melbourne Pathology | |
| Method | | Free PSA is now performed in house at Box Hill Hospital.
Chemical Pathologist approval is still required |
Recent changes for Haemoglobin Analysis (including Electrophoresis)
Recent changes for Haemoglobin Analysis (including Electrophoresis) |
Date | Field | Changed From | Changed To |
10th December 2020 | Alternative names | Alkali Resistant Haemoglobin, Foetal Haemoglobin, Hb EPG, HbA2, HbF, HbH, HbS, Unstable Haemoglobin | Alkali Resistant Haemoglobin, Foetal Haemoglobin, Hb EPG, HbA2, HbF, HbH, HbS, Unstable Haemoglobin, Thalassaemia |
| Collection & Request Instructions | | Please collect 2 X EDTA Tube for this test. |
Recent changes for Herpes Virus PCR
Recent changes for Herpes Virus PCR |
Date | Field | Changed From | Changed To |
19th October 2020 | External Transport Instructions | Tests included in Monash PCR - Herpes simplex 1 virus, Herpes simplex 2 virus, Varicella zoster virus, Cytomegalovirus, Enterovirus (swabs only), Adenovirus (not faeces)
Specimens tested at Monash-
Swabs (dry or in UTM) serum, Plasma (ACD or EDTA), Sterile fluids, Respiratory samples, Urine.
Specimens anticoagulated with heparin are unsuitable as heparin inhibits PCR
For other specimens - refer to VIDRL. Tissue, CSF, saliva.
If CSF Herpes and Enterovirus PCR -send to VIDRL.
Refer to Referred Molecular Test Requests WORK-MI-90 | Tests included in Monash PCR - Herpes simplex 1 virus, Herpes simplex 2 virus, Varicella zoster virus, Cytomegalovirus, Enterovirus (swabs only), Adenovirus (not faeces)
Specimens tested at Monash-
Swabs (dry or in UTM), Sterile fluids, Respiratory samples, Urine.
Specimens anticoagulated with heparin are unsuitable as heparin inhibits PCR
For other specimens - refer to VIDRL. Tissue, CSF, saliva, serum, Plasma (ACD or EDTA),
If CSF Herpes and Enterovirus PCR -send to VIDRL.
Refer to Referred Molecular Test Requests WORK-MI-90 |
Recent changes for Infectious Mononucleosis Screen
Recent changes for Infectious Mononucleosis Screen |
Date | Field | Changed From | Changed To |
30th March 2022 | Preferred Specimen Type | Serum | Serum Blood |
| Optional Specimen Type | | Plasma |
| Preferred Container Type | Serum tube with gel | Serum tube with gel EDTA EDTA - Paediatric |
Recent changes for Infliximab
Recent changes for Infliximab |
Date | Field | Changed From | Changed To |
5th April 2022 | Alternative names | | Inflectra, Remicade |
| Volume (Adults) | | 2 mL |
| Processing Instructions | After centifugation the serum must be aliquoted into the special aliquot tube provided and freeze. | Store at 4 C.
|
| External Laboratory | South Western Area Pathology,Liverpool Hospital | Dorevitch Pathology |
| External Transport Instructions | Samples to be batched and sent to SSWAPs frozen | Send to Testing Laboratory at 4 C |
| Frequency | Monthly | Weekly |
Recent changes for Iron - Liver
Recent changes for Iron - Liver |
Date | Field | Changed From | Changed To |
10th May 2021 | Collection & Request Instructions | Liver biopsy specimen to be placed directly into a clear, dry container and delivered to the laboraory as soon as possible. Minimum sample size for accurate analysis is 1-2 mg. | Liver biopsy specimen to be placed directly into a clear, dry container and delivered to the laboraory as soon as possible. Minimum sample size for accurate analysis is 1-2 mg. |
| External Laboratory | Australian Clinical Labs (formerly Healthscope) | Pacific Laboratory Medicine Services |
| Notes |
Hepatic Iron Index |
Hepatic Iron Concentration
|
Age in years
|
Values > 2 indicate haemochromatosis
Liver biopsy specimen to be placed directly into a clear, dry container and delivered to the laboraory as soon as possible. Minimum sample size for accurate analysis is 1-2 mg. |
Hepatic Iron Index |
Hepatic Iron Concentration
|
Age in years
|
Values > 2 indicate haemochromatosis
Liver biopsy specimen to be placed directly into a clear, dry container and delivered to the laboraory as soon as possible. Minimum sample size for accurate analysis is 1-2 mg. |
Recent changes for Itraconazole
Recent changes for Itraconazole |
Date | Field | Changed From | Changed To |
14th July 2022 | Volume (Adults) | 2 mL | 5 mL |
| Minimum/Paediatric Volume | 1 mL | |
| Preferred Container Type | Lithium heparin no gel | EDTA |
| Processing Instructions | Spin, separate and freeze plasma. | Spin and separate plasma. |
| External Laboratory | Women's & Children's Hospital (Adelaide) | Alfred Hospital |
| External Transport Instructions | Packed by certified personnal - transport frozen.
Results : 08-81617000, 08-81616732. | |
| Frequency | Monthly | Twice weekly |
| Notes | | Dose information should be provided and length of therapy must be >5 days. |
Recent changes for Karyotype/Microarray - TISSUE
Recent changes for Karyotype/Microarray - TISSUE |
Date | Field | Changed From | Changed To |
6th March 2022 | Assay name | Cytogenetics / Karyotype - TISSUE | Karyotype/Microarray - TISSUE |
14th December 2021 | Assay name | CytogeneticsKaryotype - TISSUE | Cytogenetics / Karyotype - TISSUE |
14th December 2021 | Assay name | CYTOGENETICS - TISSUE | CytogeneticsKaryotype - TISSUE |
| Alternative names | KARYOTYPE
CHROMOSOME STUDIES - TISSUE
TISSUE CULTURE
MOLECULAR KARYOTYPE
MICROARRAY | KARYOTYPE
CHROMOSOME STUDIES - TISSUE
TISSUE CULTURE
MOLECULAR KARYOTYPE
MICROARRAY
FIBROBLAST CULTURE |
14th December 2021 | Assay name | CYTOGENETICS - TISSUE NON MALIGNANT (CONSTITUTIONAL) | CYTOGENETICS - TISSUE |
| Frequency | Weekly | Daily |
Recent changes for Lamotrigine
Recent changes for Lamotrigine |
Date | Field | Changed From | Changed To |
14th July 2022 | Alternative names | | Lamictal |
| Volume (Adults) | 2 mL | 3 mL |
| Minimum/Paediatric Volume | 1 mL | 3 mL |
| Processing Instructions | Spin aliquot plasma. (Send either plasma or whole blood) | Spin & aliquot plasma |
| External Laboratory | Australian Clinical Labs (formerly Healthscope) | Alfred Hospital |
Recent changes for Leucocyte Immunophenotyping
Recent changes for Leucocyte Immunophenotyping |
Date | Field | Changed From | Changed To |
15th October 2021 | Notes | Samples must be marked 'URGENT', must reach the laboratory as soon as possible.
All out-of-hours testing requires notification.
Time and date of collection must be recorded on request form.
Whole blood - 1 x EDTA sample, do not spin. Store at room temperature. An FBE must also be requested, and needs a separate EDTA sample.
Bone marrow aspirate - Sample into EDTA tube
Tissue - Fresh (if to be shared with other departments for other tests eg. Histopathology or Microbiology) or RPMI fluid (especially if specimen is taken out of hours). This fluid is obtainable from: BH Lab 9895 3884, MH Lab: 9871 3572 or AH Lab: 9764 6136.
CSF/other fluids
Depending on the tests requested, 3-4 separate tubes will need to be provided. Note that the tubes are numbered so that order of collection can be identified – this order should be carefully followed, so that the optimal sample can be used for the requested tests. It is also important to consider the tests that are required, as this will determine how much CSF is needed in each tube. See the matrix below for details.
TUBE 1 is used for Biochemistry and Flow Cytometry. (Centrifuged in the lab – the supernatant is used for Biochemistry analysis (glucose, protein) and the cellular pellet will be used for flow cytometry if required). If tube 3 is bloodstained, take an aliquot for microbiology.
TUBE 2 is used for Cytology
TUBE 3 is for Microbiological analysis (cell count, Gram stain and culture) and any further infectious testing (e.g. viral PCR, mycobacterial PCR and culture, cryptococcal antigen).
TUBE 4 will be required only for;
(a) Creutzfeld-Jacob Disease (CJD) testing OR
(b) Xanthochromia analysis (wrap the tube in foil to prevent sample deterioration due to light).
TESTS REQUIRED
|
MINIMUM REQUIRED VOLUME (mL)
|
TUBE 1
|
TUBE 2
|
TUBE 3
|
TUBE 4
|
CHEMISTRY
|
1.0
|
|
|
|
ADD FLOW CYTOMETRY
|
Minimum 5.0-7.0
|
|
|
|
CYTOLOGY
|
|
1.0-3.0
|
|
|
MICROBIOLOGY
|
|
|
1.0-2.0
|
|
ADD OTHER INFECTIOUS MARKERS
|
|
|
add 2.0- 5.0-
|
|
ADD MYCOBACTERIAL CULTURE
|
|
|
Add 6.0-10.00
|
|
ADD CJD
|
|
|
|
1.0
|
ADD XANTHOCHROMIA
|
|
|
|
1.0 (foil)
|
Note, the ability to add further tests to a previously collected sample is dependent upon the volume provided. Also, many tests (flow cytometry, cytology) can only be performed on fresh specimens.
Other specialised testing (e.g. oligoclonal bands) will be performed using the supplied samples.
Xanthochromia and CJD analysis cannot be performed on a blood-stained sample | Samples must be marked 'URGENT', must reach the laboratory as soon as possible.
All out-of-hours testing requires notification.
Time and date of collection must be recorded on request form.
Whole blood - 1 x EDTA sample, do not spin. Store at room temperature. An FBE and BLOOD FILM must also be requested, and needs a separate EDTA sample.
Bone marrow aspirate - Sample into EDTA tube
Tissue - Fresh (if to be shared with other departments for other tests eg. Histopathology or Microbiology) or RPMI fluid (especially if specimen is taken out of hours). This fluid is obtainable from: BH Lab 9895 3884, MH Lab: 9871 3572 or AH Lab: 9764 6136.
CSF/other fluids
Depending on the tests requested, 3-4 separate tubes will need to be provided. Note that the tubes are numbered so that order of collection can be identified – this order should be carefully followed, so that the optimal sample can be used for the requested tests. It is also important to consider the tests that are required, as this will determine how much CSF is needed in each tube. See the matrix below for details.
TUBE 1 is used for Biochemistry and Flow Cytometry. (Centrifuged in the lab – the supernatant is used for Biochemistry analysis (glucose, protein) and the cellular pellet will be used for flow cytometry if required). If tube 3 is bloodstained, take an aliquot for microbiology.
TUBE 2 is used for Cytology
TUBE 3 is for Microbiological analysis (cell count, Gram stain and culture) and any further infectious testing (e.g. viral PCR, mycobacterial PCR and culture, cryptococcal antigen).
TUBE 4 will be required only for;
(a) Creutzfeld-Jacob Disease (CJD) testing OR
(b) Xanthochromia analysis (wrap the tube in foil to prevent sample deterioration due to light).
TESTS REQUIRED
|
MINIMUM REQUIRED VOLUME (mL)
|
TUBE 1
|
TUBE 2
|
TUBE 3
|
TUBE 4
|
CHEMISTRY
|
1.0
|
|
|
|
ADD FLOW CYTOMETRY
|
Minimum 5.0-7.0
|
|
|
|
CYTOLOGY
|
|
1.0-3.0
|
|
|
MICROBIOLOGY
|
|
|
1.0-2.0
|
|
ADD OTHER INFECTIOUS MARKERS
|
|
|
add 2.0- 5.0-
|
|
ADD MYCOBACTERIAL CULTURE
|
|
|
Add 6.0-10.00
|
|
ADD CJD
|
|
|
|
1.0
|
ADD XANTHOCHROMIA
|
|
|
|
1.0 (foil)
|
Note, the ability to add further tests to a previously collected sample is dependent upon the volume provided. Also, many tests (flow cytometry, cytology) can only be performed on fresh specimens.
Other specialised testing (e.g. oligoclonal bands) will be performed using the supplied samples.
Xanthochromia and CJD analysis cannot be performed on a blood-stained sample |
14th October 2021 | Notes |
Samples must be marked 'URGENT', must reach the laboratory as soon as possible.
All out-of-hours testing requires notification.
Time and date of collection must be recorded on request form.
Whole blood - 1 x EDTA sample, do not spin. Store at room temperature. An FBE must also be requested, and needs a separate EDTA sample.
Bone marrow aspirate - Sample into EDTA tube
Tissue - Fresh (if to be shared with other departments for other tests eg. Histopathology or Microbiology) or RPMI fluid (especially if specimen is taken out of hours). This fluid is obtainable from: BH Lab 9895 3884, MH Lab: 9871 3572 or AH Lab: 9764 6136.
CSF/other fluids - at least 2mL is preferred
Depending on the tests requested, 3-4 separate tubes will need to be provided. Note that the tubes are numbered so that order of collection can be identified – this order should be carefully followed, so that the optimal sample can be used for the requested tests. It is also important to consider the tests that are required, as this will determine how much CSF is needed in each tube. See the matrix below for details.
TUBE 1 is used for Biochemistry and Flow Cytometry. (Centrifuged in the lab – the supernatant is used for Biochemistry analysis (glucose, protein) and the cellular pellet will be used for flow cytometry if required). If tube 3 is bloodstained, take an aliquot for microbiology.
TUBE 2 is used for Cytology
TUBE 3 is for Microbiological analysis (cell count, Gram stain and culture) and any further infectious testing (e.g. viral PCR, mycobacterial PCR and culture, cryptococcal antigen).
TUBE 4 will be required only for;
(a) Creutzfeld-Jacob Disease (CJD) testing OR
(b) Xanthochromia analysis (wrap the tube in foil to prevent sample deterioration due to light).
TESTS REQUIRED
|
MINIMUM REQUIRED VOLUME (mL)
|
TUBE 1
|
TUBE 2
|
TUBE 3
|
TUBE 4
|
CHEMISTRY
|
1.0
|
|
|
|
ADD FLOW CYTOMETRY
|
Minimum 5.0-7.0
|
|
|
|
CYTOLOGY
|
|
1.0-3.0
|
|
|
MICROBIOLOGY
|
|
|
1.0-2.0
|
|
ADD OTHER INFECTIOUS MARKERS
|
|
|
add 2.0- 5.0-
|
|
ADD MYCOBACTERIAL CULTURE
|
|
|
Add 6.0-10.00
|
|
ADD CJD
|
|
|
|
1.0
|
ADD XANTHOCHROMIA
|
|
|
|
1.0 (foil)
|
Note, the ability to add further tests to a previously collected sample is dependent upon the volume provided. Also, many tests (flow cytometry, cytology) can only be performed on fresh specimens.
Other specialised testing (e.g. oligoclonal bands) will be performed using the supplied samples.
Xanthochromia and CJD analysis cannot be performed on a blood-stained sample |
Samples must be marked 'URGENT', must reach the laboratory as soon as possible.
All out-of-hours testing requires notification.
Time and date of collection must be recorded on request form.
Whole blood - 1 x EDTA sample, do not spin. Store at room temperature. An FBE must also be requested, and needs a separate EDTA sample.
Bone marrow aspirate - Sample into EDTA tube
Tissue - Fresh (if to be shared with other departments for other tests eg. Histopathology or Microbiology) or RPMI fluid (especially if specimen is taken out of hours). This fluid is obtainable from: BH Lab 9895 3884, MH Lab: 9871 3572 or AH Lab: 9764 6136.
CSF/other fluids
Depending on the tests requested, 3-4 separate tubes will need to be provided. Note that the tubes are numbered so that order of collection can be identified – this order should be carefully followed, so that the optimal sample can be used for the requested tests. It is also important to consider the tests that are required, as this will determine how much CSF is needed in each tube. See the matrix below for details.
TUBE 1 is used for Biochemistry and Flow Cytometry. (Centrifuged in the lab – the supernatant is used for Biochemistry analysis (glucose, protein) and the cellular pellet will be used for flow cytometry if required). If tube 3 is bloodstained, take an aliquot for microbiology.
TUBE 2 is used for Cytology
TUBE 3 is for Microbiological analysis (cell count, Gram stain and culture) and any further infectious testing (e.g. viral PCR, mycobacterial PCR and culture, cryptococcal antigen).
TUBE 4 will be required only for;
(a) Creutzfeld-Jacob Disease (CJD) testing OR
(b) Xanthochromia analysis (wrap the tube in foil to prevent sample deterioration due to light).
TESTS REQUIRED
|
MINIMUM REQUIRED VOLUME (mL)
|
TUBE 1
|
TUBE 2
|
TUBE 3
|
TUBE 4
|
CHEMISTRY
|
1.0
|
|
|
|
ADD FLOW CYTOMETRY
|
Minimum 5.0-7.0
|
|
|
|
CYTOLOGY
|
|
1.0-3.0
|
|
|
MICROBIOLOGY
|
|
|
1.0-2.0
|
|
ADD OTHER INFECTIOUS MARKERS
|
|
|
add 2.0- 5.0-
|
|
ADD MYCOBACTERIAL CULTURE
|
|
|
Add 6.0-10.00
|
|
ADD CJD
|
|
|
|
1.0
|
ADD XANTHOCHROMIA
|
|
|
|
1.0 (foil)
|
Note, the ability to add further tests to a previously collected sample is dependent upon the volume provided. Also, many tests (flow cytometry, cytology) can only be performed on fresh specimens.
Other specialised testing (e.g. oligoclonal bands) will be performed using the supplied samples.
Xanthochromia and CJD analysis cannot be performed on a blood-stained sample |
14th October 2021 | Alternative names | CD4/CD8 Counts, Cell Surface Markers, FACS Analysis, Flow Cytometry | Cell Surface Markers, FACS Analysis, Flow Cytometry, Immunophenotyping |
| Request Group | FLOW | LYMP, LEUK |
| Collection & Request Instructions | Sample store at Room temperature. Samples are not stable for more than 24 hours. Hence they must be received by the Flow Cytometry lab at Box Hill within normal business hours Monday to Friday.
Cutoff for outpatient sample collection on Fridays is at 11.30 AM and MUST reach the laboratory before 4.00PM.
Flow cytometric analysis of cells stained by immunofluorescence with a range of antibodies against lymphoid and myeloid markers.
The panel of markers used will be appropriate to the clinical comments supplied , or as arranged by prior consultation. | Sample store at Room temperature. Samples are not stable for more than 24 hours. Hence they must be received by the Flow Cytometry lab at Box Hill within normal business hours Monday to Friday.
Cut-off for outpatient sample collection on Fridays is at 11.30 AM and MUST reach the laboratory before 4.00PM.
Flow cytometric analysis of cells stained by immunofluorescence with a range of antibodies against lymphoid and myeloid markers.
The panel of markers used will be appropriate to the panel selected and the clinical comments supplied , or as arranged by prior consultation |
| Notes |
Samples must be marked 'URGENT', must reach the laboratory as soon as possible.
All out-of-hours testing requires notification.
Time and date of collection must be recorded on request form.
Whole blood - 1 x EDTA sample, do not spin. Store at room temperature. An FBE must also be requested, needs a separate EDTA sample.
Bone marrow aspirate - Sample into EDTA tube
Tissue - Fresh (if to be shared with other departments for other tests eg. Histopathology or Microbiology) or RPMI fluid (especially if specimen is taken out of hours). This fluid is obtainable from: BH Lab 9895 3884, MH Lab: 9871 3572 or AH Lab: 9764 6136.
CSF/other fluids - at least 2mL is preferred
Collect CSF into three separate, sterile, screw-capped tubes provided (labelled 1,2 & 3)
Tube 1 is for Biochemistry (for Protein and Glucose) 0.5ml (10 drops) required
Tube 2 for Cytology 1.0ml (20 drops) and/or Flow Cytometry (6ml)
For Flow Cytometry a minimum of 6ml is required in Tube 2
For Flow Cytometry and Cytology a minimum of 7ml is required in Tube 2
Tube 3 for Microbiology upto 10ml (Full tube)
If querying CJD the laboratory requires a 4th unopened tube containing at least 1ml of non-bloodstained CSF.
Xanthochromia investigation requires a 4th tube containing 1ml of CSF . |
Samples must be marked 'URGENT', must reach the laboratory as soon as possible.
All out-of-hours testing requires notification.
Time and date of collection must be recorded on request form.
Whole blood - 1 x EDTA sample, do not spin. Store at room temperature. An FBE must also be requested, and needs a separate EDTA sample.
Bone marrow aspirate - Sample into EDTA tube
Tissue - Fresh (if to be shared with other departments for other tests eg. Histopathology or Microbiology) or RPMI fluid (especially if specimen is taken out of hours). This fluid is obtainable from: BH Lab 9895 3884, MH Lab: 9871 3572 or AH Lab: 9764 6136.
CSF/other fluids - at least 2mL is preferred
Depending on the tests requested, 3-4 separate tubes will need to be provided. Note that the tubes are numbered so that order of collection can be identified – this order should be carefully followed, so that the optimal sample can be used for the requested tests. It is also important to consider the tests that are required, as this will determine how much CSF is needed in each tube. See the matrix below for details.
TUBE 1 is used for Biochemistry and Flow Cytometry. (Centrifuged in the lab – the supernatant is used for Biochemistry analysis (glucose, protein) and the cellular pellet will be used for flow cytometry if required). If tube 3 is bloodstained, take an aliquot for microbiology.
TUBE 2 is used for Cytology
TUBE 3 is for Microbiological analysis (cell count, Gram stain and culture) and any further infectious testing (e.g. viral PCR, mycobacterial PCR and culture, cryptococcal antigen).
TUBE 4 will be required only for;
(a) Creutzfeld-Jacob Disease (CJD) testing OR
(b) Xanthochromia analysis (wrap the tube in foil to prevent sample deterioration due to light).
TESTS REQUIRED
|
MINIMUM REQUIRED VOLUME (mL)
|
TUBE 1
|
TUBE 2
|
TUBE 3
|
TUBE 4
|
CHEMISTRY
|
1.0
|
|
|
|
ADD FLOW CYTOMETRY
|
Minimum 5.0-7.0
|
|
|
|
CYTOLOGY
|
|
1.0-3.0
|
|
|
MICROBIOLOGY
|
|
|
1.0-2.0
|
|
ADD OTHER INFECTIOUS MARKERS
|
|
|
add 2.0- 5.0-
|
|
ADD MYCOBACTERIAL CULTURE
|
|
|
Add 6.0-10.00
|
|
ADD CJD
|
|
|
|
1.0
|
ADD XANTHOCHROMIA
|
|
|
|
1.0 (foil)
|
Note, the ability to add further tests to a previously collected sample is dependent upon the volume provided. Also, many tests (flow cytometry, cytology) can only be performed on fresh specimens.
Other specialised testing (e.g. oligoclonal bands) will be performed using the supplied samples.
Xanthochromia and CJD analysis cannot be performed on a blood-stained sample |
20th July 2021 | Assay name | Leucocyte Immunophenotyping | T Cell Subsets |
| Alternative names | CD4/CD8 Counts, Cell Surface Markers, FACS Analysis, Flow Cytometry | CD2, CD3, CD4, CD8 |
| Preferred Container Type | EDTA Sterile Jar | EDTA Lithium heparin no gel |
| Collection & Request Instructions | Sample store at Room temperature. Samples are not stable for more than 24 hours. Hence they must be received by the Flow Cytometry lab at Box Hill within normal business hours Monday to Friday.
Cutoff for outpatient sample collection on Fridays is at 11.30 AM and MUST reach the laboratory before 4.00PM.
Flow cytometric analysis of cells stained by immunofluorescence with a range of antibodies against lymphoid and myeloid markers.
The panel of markers used will be appropriate to the clinical comments supplied , or as arranged by prior consultation. | The samples are not stable for more than 24 hours. Hence all samples must be recieved in the laboratory at Box Hill Monday to Friday within business hours.
Cutoff for outpatient sample collection on Fridays is at 11.30 AM and MUST reach the laboratory before 4.00PM. |
| Processing Instructions | | Prefer EDTA sample.
Do not spin, store at room temperature. |
| Notes |
Samples must be marked 'URGENT', must reach the laboratory as soon as possible.
All out-of-hours testing requires notification.
Time and date of collection must be recorded on request form.
Whole blood - 1 x EDTA sample, do not spin. Store at room temperature. An FBE must also be requested, needs a separate EDTA sample.
Bone marrow aspirate - Sample into EDTA tube
Tissue - Fresh (if to be shared with other departments for other tests eg. Histopathology or Microbiology) or RPMI fluid (especially if specimen is taken out of hours). This fluid is obtainable from: BH Lab 9895 3884, MH Lab: 9871 3572 or AH Lab: 9764 6136.
CSF/other fluids - at least 2mL is preferred
Collect CSF into three separate, sterile, screw-capped tubes provided (labelled 1,2 & 3)
Tube 1 is for Biochemistry (for Protein and Glucose) 0.5ml (10 drops) required
Tube 2 for Cytology 1.0ml (20 drops) and/or Flow Cytometry (6ml)
For Flow Cytometry a minimum of 6ml is required in Tube 2
For Flow Cytometry and Cytology a minimum of 7ml is required in Tube 2
Tube 3 for Microbiology upto 10ml (Full tube)
If querying CJD the laboratory requires a 4th unopened tube containing at least 1ml of non-bloodstained CSF.
Xanthochromia investigation requires a 4th tube containing 1ml of CSF . | Used to monitor T-cell numbers
Preferred EDTA sample. Do not spin, store at room temperature. |
19th July 2021 | Collection & Request Instructions | Sample store at Room temperature. Samples are not stable for more than 24 hours. Hence they must be recieved by the Flow Cytometry lab at Box Hill within normal business hours Monday to Friday.
Cutoff for outpatirnt sample collection on Fridays is at 12:00 PM and MUST reach the laboratory by 4.00PM.
Flow cytometric analysis of cells stained by immunofluorescence with a range of antibodies against lymphoid and myeloid markers.
The panel of markers used will be appropriate to the clinical comments supplied , or as arranged by prior consultation. | Sample store at Room temperature. Samples are not stable for more than 24 hours. Hence they must be received by the Flow Cytometry lab at Box Hill within normal business hours Monday to Friday.
Cutoff for outpatient sample collection on Fridays is at 11.30 AM and MUST reach the laboratory before 4.00PM.
Flow cytometric analysis of cells stained by immunofluorescence with a range of antibodies against lymphoid and myeloid markers.
The panel of markers used will be appropriate to the clinical comments supplied , or as arranged by prior consultation. |
19th July 2021 | Collection & Request Instructions |
Sample store at Room temperature. Samples are not stable for more than 24 hours. Hence they must be recieved by the Flow Cytometry lab at Box Hill within normal business hours Monday to Friday
Flow cytometric analysis of cells stained by immunofluorescence with a range of antibodies against lymphoid and myeloid markers.
The panel of markers used will be appropriate to the clinical comments supplied , or as arranged by prior consultation. | Sample store at Room temperature. Samples are not stable for more than 24 hours. Hence they must be recieved by the Flow Cytometry lab at Box Hill within normal business hours Monday to Friday.
Cutoff for outpatirnt sample collection on Fridays is at 12:00 PM and MUST reach the laboratory by 4.00PM.
Flow cytometric analysis of cells stained by immunofluorescence with a range of antibodies against lymphoid and myeloid markers.
The panel of markers used will be appropriate to the clinical comments supplied , or as arranged by prior consultation. |
| Notes |
-
Samples must be marked 'URGENT', must reach the laboratory as soon as possible.
-
All out-of-hours testing requires notification.
-
Time and date of collection must be recorded on request form.
Whole blood - 1 x EDTA sample, do not spin. Store at room temperature. An FBE must also be requested, needs a separate EDTA sample.
Bone marrow aspirate - Sample into EDTA tube
Tissue - Fresh (if to be shared with other departments for other tests eg. Histopathology or Microbiology) or RPMI fluid (especially if specimen is taken out of hours). This fluid is obtainable from: BH Lab 9895 3884, MH Lab: 9871 3572 or AH Lab: 9764 6136.
-
CSF/other fluids - at least 2mL is preferred
-
Collect CSF into three separate, sterile, screw-capped tubes provided (labelled 1,2 & 3)
-
Tube 1 is for Biochemistry (for Protein and Glucose) 0.5ml (10 drops) required
-
Tube 2 for Cytology 1.0ml (20 drops) and/or Flow Cytometry (6ml)
-
For Flow Cytometry a minimum of 6ml is required in Tube 2
-
For Flow Cytometry and Cytology a minimum of 7ml is required in Tube 2
-
Tube 3 for Microbiology upto 10ml (Full tube)
If querying CJD the laboratory requires a 4th unopened tube containing at least 1ml of non-bloodstained CSF.
Xanthochromia investigation requires a 4th tube containing 1ml of CSF .
|
Samples must be marked 'URGENT', must reach the laboratory as soon as possible.
All out-of-hours testing requires notification.
Time and date of collection must be recorded on request form.
Whole blood - 1 x EDTA sample, do not spin. Store at room temperature. An FBE must also be requested, needs a separate EDTA sample.
Bone marrow aspirate - Sample into EDTA tube
Tissue - Fresh (if to be shared with other departments for other tests eg. Histopathology or Microbiology) or RPMI fluid (especially if specimen is taken out of hours). This fluid is obtainable from: BH Lab 9895 3884, MH Lab: 9871 3572 or AH Lab: 9764 6136.
CSF/other fluids - at least 2mL is preferred
Collect CSF into three separate, sterile, screw-capped tubes provided (labelled 1,2 & 3)
Tube 1 is for Biochemistry (for Protein and Glucose) 0.5ml (10 drops) required
Tube 2 for Cytology 1.0ml (20 drops) and/or Flow Cytometry (6ml)
For Flow Cytometry a minimum of 6ml is required in Tube 2
For Flow Cytometry and Cytology a minimum of 7ml is required in Tube 2
Tube 3 for Microbiology upto 10ml (Full tube)
If querying CJD the laboratory requires a 4th unopened tube containing at least 1ml of non-bloodstained CSF.
Xanthochromia investigation requires a 4th tube containing 1ml of CSF . |
Recent changes for Linezolid
Recent changes for Linezolid |
Date | Field | Changed From | Changed To |
14th July 2022 | Volume (Adults) | 2 mL | 3 mL |
| Minimum/Paediatric Volume | | 3 mL |
| Optional Container Type | | Lithium heparin no gel |
| Collection & Request Instructions | Deliver to laboratory ASAP. | A separate unopened specimen is required for this test. |
| Processing Instructions | Spin, separate and freeze plasma. | |
| External Laboratory | Sydpath Pathology, St Vincent's Hospital NSW | Alfred Hospital |
| External Transport Instructions | | Send at 4 C. |
| Frequency | As required | Weekly |
| Method | Results: Clinical Pharmacology on 02 8382 9184. | |
Recent changes for Lupus Anticoagulant Screen
Recent changes for Lupus Anticoagulant Screen |
Date | Field | Changed From | Changed To |
3rd May 2021 | External Transport Instructions | Samples can be transported frozen to the laboratory if the facilities are available to double spin and aliquot these sample. This has to be done within 4 hours.
Unspun sample is stable for up to 4 hours and must be transport to laboratory immediately. (Sample must be double spun, aliquoted and frozen within 4 hours from time of collection). | Samples can be transported frozen to the laboratory if the facilities are available to double spin and aliquot these samples. This has to be performed within 4 hours of collection.
Unspun sample is stable for up to 4 hours and must be transport to laboratory immediately. (Sample must be double spun, aliquoted and frozen within 4 hours from time of collection). |
3rd May 2021 | Collection & Request Instructions | Notification of anticoagulant therapy is essential
One (1x) Sodium citrate tubes is required. Do not use 1 ml citrate tubes.
Filled to label marker and mixed by inversion
The patient must not be receiving heparin
Transport to laboratory immediately. (Sample must be double spun and frozen within 4 hours from time of collection).
Tests performed:
INR, APTT, APTT Mix, Silica Clotting Time and Dilute Russell's Viper venom time (LA screen/Confirm).
| Notification of anticoagulant therapy is essential
One (1x) Sodium citrate tubes is required. Do not use 1 ml citrate tubes.
Filled to label marker and mixed by inversion
The patient must not be receiving heparin
Unspun sample is stable for up to 4 hours and must be transport to laboratory immediately. (Sample must be double spun, aliquoted and frozen within 4 hours from time of collection).
Tests performed:
INR, APTT, APTT Mix, Silica Clotting Time and Dilute Russell's Viper venom time (LA screen/Confirm).
|
| Processing Instructions | Transport sample to laboratory within 4 hours of collection.
The sample MUST be double spun and frozen within the 4 hour time limit. | Unspun sample is stable for up to 4 hours and must be transport to laboratory immediately. (Sample must be double spun, aliquoted and frozen within 4 hours from time of collection). |
| External Transport Instructions | | Samples can be transported frozen to the laboratory if the facilities are available to double spin and aliquot these sample. This has to be done within 4 hours.
Unspun sample is stable for up to 4 hours and must be transport to laboratory immediately. (Sample must be double spun, aliquoted and frozen within 4 hours from time of collection). |
3rd May 2021 | Collection & Request Instructions | Notification of anticoagulant therapy is essential
One (1x) Sodium citrate tubes is required. Do not use 1 ml citrate tubes.
Filled to label marker and mixed by inversion
The patient must not be receiving heparin
Transport to laboratory immediately. (maximum of 4 hours from time of collection).
Tests performed:
INR, APTT, APTT Mix, Silica Clotting Time and Dilute Russell's Viper venom time (LA screen/Confirm).
| Notification of anticoagulant therapy is essential
One (1x) Sodium citrate tubes is required. Do not use 1 ml citrate tubes.
Filled to label marker and mixed by inversion
The patient must not be receiving heparin
Transport to laboratory immediately. (Sample must be double spun and frozen within 4 hours from time of collection).
Tests performed:
INR, APTT, APTT Mix, Silica Clotting Time and Dilute Russell's Viper venom time (LA screen/Confirm).
|
| Processing Instructions | | Transport sample to laboratory within 4 hours of collection.
The sample MUST be double spun and frozen within the 4 hour time limit. |
19th January 2021 | Method | Centrifuge the two 3.5 mL citrate tubes and transfer the plasma to a labeled 4 mL plastic tube and re-centrifuge at room temperature (do not use the refrigerated centrifuge).
The double centrifuged plasma is transferred to two 1 mL eppendorf tubes and freeze.
Sample must be centrifuged within 4 hours of collection. | **Sample must be centrifuged within 4 hours of collection**
Centrifuge the two 3.5 mL citrate tubes (and transfer the plasma to a labeled 4 mL plastic tube and re-centrifuge at room temperature (do not use the refrigerated centrifuge). One sample is acceptable but 2 samples are preferable for Lupus testing.
The double centrifuged plasma is transferred into a new labelled tubes.
Double spun samples are then frozen in the -20'C freezer. |
19th January 2021 | Collection & Request Instructions | Notification of anticoagulant therapy is essential
One (1x) Sodium citrate tubes is required. Do not use 1 ml citrate tubes.
Filled to label marker and mixed by inversion
The patient must not be receiving heparin
Transport to laboratory immediately.
Tests performed:
INR, APTT, APTT Mix, Lupus sensitive APTT and Dilute Russell's Viper venom time (LA screen/Confirm).
| Notification of anticoagulant therapy is essential
One (1x) Sodium citrate tubes is required. Do not use 1 ml citrate tubes.
Filled to label marker and mixed by inversion
The patient must not be receiving heparin
Transport to laboratory immediately. (maximum of 4 hours from time of collection).
Tests performed:
INR, APTT, APTT Mix, Silica Clotting Time and Dilute Russell's Viper venom time (LA screen/Confirm).
|
| Method | Centrifuge the two 3.5 mL citrate tubes and transfer the plasma to a labeled 4 mL plastic tube and re-centrifuge at room temperature (do not use the refrigerated centrifuge).
The double centrifuged plasma is transferred to two 1 mL eppendorf tubes and freeze. | Centrifuge the two 3.5 mL citrate tubes and transfer the plasma to a labeled 4 mL plastic tube and re-centrifuge at room temperature (do not use the refrigerated centrifuge).
The double centrifuged plasma is transferred to two 1 mL eppendorf tubes and freeze.
Sample must be centrifuged within 4 hours of collection. |
10th December 2020 | Collection & Request Instructions | Notification of anticoagulant therapy is essential
Two (2x) Sodium citrate tubes required. Do not use 1 ml citrate tubes.
Filled to label marker and mixed by inversion
The patient must not be receiving heparin
Transport to laboratory immediately.
Tests performed:
INR, APTT, APTT Mix, Lupus sensitive APTT and Dilute Russell's Viper venom time (LA screen/Confirm).
| Notification of anticoagulant therapy is essential
One (1x) Sodium citrate tubes is required. Do not use 1 ml citrate tubes.
Filled to label marker and mixed by inversion
The patient must not be receiving heparin
Transport to laboratory immediately.
Tests performed:
INR, APTT, APTT Mix, Lupus sensitive APTT and Dilute Russell's Viper venom time (LA screen/Confirm).
|
Recent changes for Lymphocyte subsets
Recent changes for Lymphocyte subsets |
Date | Field | Changed From | Changed To |
14th October 2021 | Alternative names | | CD3 count, CD4 count, CD8 count, CD4:CD8 ratio
CD19 count, CD56 count, flow cytometry |
14th October 2021 | Alternative names | CD19 count & CD56 count, CD4 count, CD4 count, CD4:CD8 ratio
Lymphocyte subsets include: CD3 count, CD4:CD8 ratio, CD8 count, CD8 count, T subsets include: CD3 count | T subsets include: CD3 count, CD4 count, CD8 count, CD4:CD8 ratio
Lymphocyte subsets include: CD3 count, CD4 count, CD8 count, CD4:CD8 ratio, CD19 count & CD56 count
Flow Cytometry |
| Collection & Request Instructions | Sample store at Room temperature. Samples are not stable for more than 24 hours. Hence they must be received by the Flow Cytometry lab at Box Hill within normal business hours Monday to Friday.
Cut-off for outpatient sample collection on Fridays is at 11.30 AM and MUST reach the laboratory before 4.00PM.
Flow cytometric analysis of cells stained by immunofluorescence with a range of antibodies against lymphoid and myeloid markers.
The panel of markers used will be appropriate to the panel selected and the clinical comments supplied, or as arranged by prior consultation. | Sample store at ROOM TEMPERATURE. Samples are not stable for more than 24 hours. Hence they must be received by the Flow Cytometry lab at Box Hill within normal business hours Monday to Friday.
Cut-off for outpatient sample collection on Fridays is at 11.30 AM and MUST reach the laboratory before 4.00PM.
Flow cytometric analysis of cells stained by immunofluorescence with a range of antibodies against lymphoid and myeloid markers.
The panel of markers used will be appropriate to the panel selected and the clinical comments supplied, or as arranged by prior consultation. |
14th October 2021 | Collection & Request Instructions | The samples are not stable for more than 24 hours. Hence all samples must be recieved in the laboratory at Box Hill Monday to Friday within business hours.
Cutoff for outpatient sample collection on Fridays is at 11.30 AM and MUST reach the laboratory before 4.00PM.
| Sample store at Room temperature. Samples are not stable for more than 24 hours. Hence they must be received by the Flow Cytometry lab at Box Hill within normal business hours Monday to Friday.
Cut-off for outpatient sample collection on Fridays is at 11.30 AM and MUST reach the laboratory before 4.00PM.
Flow cytometric analysis of cells stained by immunofluorescence with a range of antibodies against lymphoid and myeloid markers.
The panel of markers used will be appropriate to the panel selected and the clinical comments supplied, or as arranged by prior consultation. |
| Frequency | | Daily |
| Notes | Used to monitor T-cell numbers
Preferred EDTA sample. Do not spin, store at room temperature. | Samples must be marked 'URGENT', must reach the laboratory as soon as possible.
All out-of-hours testing requires notification.
Time and date of collection must be recorded on request form.
Whole blood - 1 x EDTA sample, do not spin. Store at room temperature. An FBE must also be requested, and needs a separate EDTA sample. |
14th October 2021 | Assay name | T Cell Subsets | Lymphocyte subsets |
| Alternative names | CD2, CD3, CD4, CD8 | T subsets include: CD3 count, CD4 count, CD8 count, CD4:CD8 ratio
Lymphocyte subsets include: CD3 count, CD4 count, CD8 count, CD4:CD8 ratio, CD19 count & CD56 count |
| Request Group | FLOW | TSUB & LSUB |
20th July 2021 | Processing Instructions | Prefer EDTA sample.
Do not spin, store at room temperature. | Prefer EDTA sample.
Do not spin, store at room temperature. |
20th July 2021 | Notes | Used to monitor T-cell numbers
Preferred EDTA sample. Do not spin, store at room temperature. | Used to monitor T-cell numbers
Preferred EDTA sample. Do not spin, store at room temperature. |
20th July 2021 | Collection & Request Instructions |
The samples are not stable for more than 24 hours. Hence all samples must be recieved in the laboratory at Box Hill Monday to Friday within business hours. | The samples are not stable for more than 24 hours. Hence all samples must be recieved in the laboratory at Box Hill Monday to Friday within business hours.
Cutoff for outpatient sample collection on Fridays is at 11.30 AM and MUST reach the laboratory before 4.00PM.
|
| Processing Instructions |
Prefer EDTA sample.
Do not spin, store at room temperature. | Prefer EDTA sample.
Do not spin, store at room temperature. |
| Notes |
Used to monitor T-cell numbers
Preferred EDTA sample. Do not spin, store at room temperature. | Used to monitor T-cell numbers
Preferred EDTA sample. Do not spin, store at room temperature. |
Recent changes for Mycobacterium ulcerans PCR
Recent changes for Mycobacterium ulcerans PCR |
Date | Field | Changed From | Changed To |
3rd March 2021 | Alternative names | Mycobacterium ulcerans NAD | Mycobacterium ulcerans NAD, Buruli ulcer, Bairnsdale ulcer |
| Collection & Request Instructions |
Specimen Required: Fresh tissue biopsy, dry swabs and paraffin-embedded fixed tissue sections (at least 6 sections, each 10-20 mm thick) | Specimen Required: Fresh tissue biopsy, dry swabs (collect x2 - one for Mycobacterial culture and one for M.ulcerans PCR) and paraffin-embedded fixed tissue sections (at least 6 sections, each 10-20 mm thick). A gel swab may be used if unable to obtain a dry swab. |
Recent changes for Paroxysmal Nocturnal Haemoglobinuria
Recent changes for Paroxysmal Nocturnal Haemoglobinuria |
Date | Field | Changed From | Changed To |
15th October 2021 | Collection & Request Instructions | Separate specimen required.
Cutoff for outpatient sample collection on Fridays is at 11.30 AM and MUST reach the laboratory before 4.00PM. | Whole blood - 1 x EDTA sample, do not spin. Store at room temperature. An FBE and BLOOD FILM must also be requested, and needs a separate EDTA sample.
Cutoff for outpatient sample collection on Fridays is at 11.30 AM and MUST reach the laboratory before 4.00PM. |
12th October 2021 | Alternative names | CD 59, PNH flow cytometry, PNH screen | CD59, PNH screen flow cytometry, FLEAR |
12th October 2021 | Alternative names | CD 59, PNH screen | CD 59, PNH screen, PNH flow cytometry |
| Request Group | FLOW | PNHS |
20th July 2021 | Collection & Request Instructions |
Separate specimen required. | Separate specimen required.
Cutoff for outpatient sample collection on Fridays is at 11.30 AM and MUST reach the laboratory before 4.00PM. |
Recent changes for Plasminogen
Recent changes for Plasminogen |
Date | Field | Changed From | Changed To |
21st December 2020 | Volume (Adults) | 4 mL | 3.5 mL |
17th December 2020 | External Laboratory | Austin Pathology | Monash Pathology |
Recent changes for Procalcitonin
Recent changes for Procalcitonin |
Date | Field | Changed From | Changed To |
17th August 2022 | Method | | All PCT requests must be discussed with the Chemical Pathologist upon receipt in lab, regardless of time of day or day of week. If urgent/Taxi requiring is determined, result must be phoned to the clinician upon receipt - High Risk Result. |
27th July 2022 | Laboratory | Biochemistry | Referred Test |
| Volume (Adults) | | 0.5 mL |
| External Laboratory | | Royal Childrens Hospital |
Recent changes for Protein C
Recent changes for Protein C |
Date | Field | Changed From | Changed To |
19th January 2021 | Collection & Request Instructions | Transport specimen at Room Temperature to lab | |
| Collection & Request Instructions | Fill to label marker and mix by inversion.
Do not use 1 mL citrate tubes.
1 x 3.5 ml citrate tubes sufficient for Protein C, Protein S and Antithrombin 3. | Fill to label marker and mix by inversion.
Do not use 1 mL citrate tubes.
Samples must be transported to laboratory immediately. ( Within a maximun of 4 hours from collection time)
1 x 3.5 ml citrate tubes sufficient for Protein C, Protein S and Antithrombin 3. |
10th December 2020 | Volume (Adults) | | 3.5 mL |
10th December 2020 | Collection & Request Instructions | Fill to label marker and mix by inversion.
Do not use 1 mL citrate tubes.
2 x 3.5 ml citrate tubes sufficient for Protein C, Protein S and Antithrombin 3. | Fill to label marker and mix by inversion.
Do not use 1 mL citrate tubes.
1 x 3.5 ml citrate tubes sufficient for Protein C, Protein S and Antithrombin 3. |
| Notes | Results not valid if patient receiving warfarin therapy or with acute thrombotic event.
Warfarin falsely lowers Protein C levels.
Fill to label marker and mix by inversion.
Do not use 1 mL citrate tubes.
2 x 3.5 ml citrate sufficient for Protein C, Protein S and Antithrombin 3. | Results not valid if patient receiving warfarin therapy or with acute thrombotic event.
Warfarin falsely lowers Protein C levels.
Fill to label marker and mix by inversion.
Do not use 1 mL citrate tubes.
1 x 3.5 ml citrate sufficient for Protein C, Protein S and Antithrombin 3. |
Recent changes for Protein S
Recent changes for Protein S |
Date | Field | Changed From | Changed To |
19th January 2021 | Collection & Request Instructions | Fill to label marker and mix by inversion
Do not use 1 ml citrate tubes.
1 x 3.5 mL citrate tubes sufficient for Protein C, Protein S and Antithrombin 3. | Fill to label marker and mix by inversion
Do not use 1 ml citrate tubes.
Samples must be transported to laboratory immediately. ( Within a maximun of 4 hours from collection time)
1 x 3.5 mL citrate tubes sufficient for Protein C, Protein S and Antithrombin 3. |
10th December 2020 | Volume (Adults) | 7 mL | 3.5 mL |
10th December 2020 | Collection & Request Instructions | Fill to label marker and mix by inversion
Do not use 1 ml citrate tubes.
2 x 3.5 mL citrate tubes sufficient for Protein C, Protein S and Antithrombin 3. | Fill to label marker and mix by inversion
Do not use 1 ml citrate tubes.
1 x 3.5 mL citrate tubes sufficient for Protein C, Protein S and Antithrombin 3. |
| Notes | Results not valid if patient receiving warfarin therapy or with acute thrombotic event
Warfarin falsely lowers Protein S level.
Fill to label marker and mix by inversion
Do not use 1 ml citrate tubes.
2 x 3.5 mL citrate tubes sufficient for Protein C, Protein S and Antithrombin 3. | Results not valid if patient receiving warfarin therapy or with acute thrombotic event
Warfarin falsely lowers Protein S level.
Fill to label marker and mix by inversion
Do not use 1 ml citrate tubes.
1 x 3.5 mL citrate tubes sufficient for Protein C, Protein S and Antithrombin 3. |
Recent changes for Prothrombin Time
Recent changes for Red Cell Antigen Genotyping
Recent changes for Red Cell Antigen Genotyping |
Date | Field | Changed From | Changed To |
9th May 2022 | Collection & Request Instructions |
Minimum volume of 4 ml required - this specimen cannot be used for any other purpose.
A completed "ARCBS Red Cell Reference Form" must accompany each sample sent for analysis.
This form, which can be completed online and subsequently printed to be sent with the sample can be found by clicking here | Minimum volume of 4 ml required - this specimen cannot be used for any other purpose.
A completed "ARCBS Red Cell Reference Form" must accompany each sample sent for analysis.
This form, which can be completed online and subsequently printed to be sent with the sample can be found by clicking here |
| Processing Instructions |
Specimen will arrive in department at RT. Please keep at 4 degrees prior to transport. | Specimen will arrive in department at RT. Please keep at 4 degrees prior to transport. |
| External Transport Instructions |
Transport at 4 degrees | Transport at 4 degrees |
Recent changes for Risperidone
Recent changes for Risperidone |
Date | Field | Changed From | Changed To |
18th December 2020 | External Laboratory | Pathwest Lab Med WA | Alfred Hospital |
Recent changes for Ristocetin Co-Factor assay
Recent changes for Ristocetin Co-Factor assay |
Date | Field | Changed From | Changed To |
11th June 2021 | Alternative names | RICOF | RICOF, vWF:Activity, vWF:Act |
| Laboratory | Referred Test | Coagulation |
| External Laboratory | Austin Pathology | |
Recent changes for Scabies investigation
Recent changes for Scabies investigation |
Date | Field | Changed From | Changed To |
8th June 2021 | Collection & Request Instructions | Scabies collection instructions
Preparation
Wash the area of skin to be scraped with an alcohol swab.
Remove any ointment or residual applications using soap and water.
Disinfect the area with a skin cleansing alcohol wipe and allow it to dry.
Abrade the infested area with a sterile scalpel to the depth of pinpoint bleeding.
Transfer skin scrapings to a sterile yellow top pot for transport to the laboratory. Do NOT include the scalpel – dispose of in sharps bin
Label pot with patient name, UR No and DOB, date of collection, site of collection | Preparation
Wash the area of skin to be scraped with an alcohol swab.
Remove any ointment or residual applications using soap and water.
Disinfect the area with a skin cleansing alcohol wipe and allow it to dry.
Abrade the infested area with a sterile scalpel to the depth of pinpoint bleeding.
Transfer skin scrapings to a sterile yellow top pot for transport to the laboratory. Do NOT include the scalpel – dispose of in sharps bin
Label pot with patient name, UR No and DOB, date of collection, site of collection |
Recent changes for Test
Recent changes for Test |
Date | Field | Changed From | Changed To |
3rd December 2020 | Method | Laboratory notes testing | Laboratory notes - email test |
Recent changes for Thrombophilia Screen
Recent changes for Thrombophilia Screen |
Date | Field | Changed From | Changed To |
10th December 2020 | Collection & Request Instructions | For full thrombophilia screen including INR, APTT, Protein C, Protein S, AT3, LA screen, APCR, Homocystine, Factor V Leiden, PG20210A and ACA:-
5 x 3.5 ml citrate tubes plus 2 EDTA tubes and 1 x serum tube will be required. | For full thrombophilia screen including INR, APTT, Protein C, Protein S, AT3, LA screen, , Homocystine, Factor V Leiden, PG20210A and ACA/ACL antibodies:-
3 x 3.5 ml citrate tubes plus 2 EDTA tubes and 1 x serum tube will be required. |
| Notes | May include Protein C functional, Protein S free, Antithrombin III, APCR ratio, Factor V Leiden, Homocystine, Anti-cardiolipin antibodies, Lupus Anticoagulant, Prothrombin mutation.
Which tests are most appropriate depends on clinical history
Tests need to be listed individually to comply with HIC guidelines
Consult Haematology Registrar or Haematologist.
For full thrombophilia screen including INR, APTT, Protein C, Protein S, AT3, LA screen, APCR, Homocystine, Factor V Leiden, PG20210A and ACA:- 5 x 3.5 ml citrate tubes plus 2 EDTA tubes and 1 x serum tube will be required. | May include Protein C functional, Protein S free, Antithrombin III, Factor V Leiden, Homocystine, Anti-cardiolipin antibodies, Lupus Anticoagulant, Prothrombin Gene Mutation.
Which tests are most appropriate depends on clinical history
Tests need to be listed individually to comply with HIC guidelines
Consult Haematology Registrar or Haematologist.
For full thrombophilia screen including INR, APTT, Protein C, Protein S, AT3, LA screen, Homocystine, Factor V Leiden, PG20210A and ACA:- 3 x 3.5 ml citrate tubes plus 2 EDTA tubes and 1 x serum tube will be required. |
Recent changes for Tissue release from Anatomical Pathology
Recent changes for Vitamin C
Recent changes for Vitamin C |
Date | Field | Changed From | Changed To |
31st March 2022 | Volume (Adults) | 2 mL | 1 mL |
| Minimum/Paediatric Volume | | 1 mL |
| Processing Instructions | Spin, separate, wrap in foil and freeze ASAP.
Transport frozen. Contact Gribbles to arrange courier. | Spin, separate, wrap in foil and freeze ASAP.
Transport frozen. |
| External Laboratory | Australian Clinical Labs (formerly Healthscope) | Alfred Hospital |
| Frequency | Weekly | Fortnightly |
Recent changes for Xanthochromia
Recent changes for Xanthochromia |
Date | Field | Changed From | Changed To |
14th July 2021 | Laboratory | Referred Test | Biochemistry |
| Collection & Request Instructions | Please note : to avoid sample deterioration, DO NOT send CSF specimens via the vacuum chute system.
Please quickly check that the tube is labelled underneath the foil before referring.
THERE MUST BE A MINIMUM OF 0.6 mL OF CSF OR THE TEST CANNOT BE PERFORMED
| Please note : to avoid sample deterioration, DO NOT send CSF specimens via the vacuum chute system.
THERE MUST BE A MINIMUM OF 0.6 mL OF CSF OR THE TEST CANNOT BE PERFORMED
|
| Processing Instructions | CSF xanthochromia checklist
Sample collected >12hrs from onset symptoms Preferable
4th CSF sample Essential
Sample light protected immediately Essential
Sample transported manually to lab Essential
Serum sample for bilirubin & albumin Preferable
Slip with clinical notes and contact details Essential
| CSF xanthochromia checklist
Sample collected >12hrs from onset symptoms Preferable
4th CSF sample Essential
Sample light protected immediately Essential
Sample transported manually to lab Essential
Serum sample for bilirubin & protein- essemtial
Slip with clinical notes and contact details Essential
|
| External Laboratory | Austin Pathology | |
| External Transport Instructions | Send via a Taxi ASAP. Ring Austin Pathology on 94963100. | MH and AH send via taxi ASAP and notify BH laboraotry. |
| Frequency | Daily | As required |
| Method | | Please quickly check that the tube is labelled underneath the foil before passing to Scientist.
The specimen designated for spectrophotometry should be centrifuged at .2000 rpm for 5 min as soon as possible after receipt in the laboratory. The supernatant must be stored in the dark at 40 C until analysis. |
| Notes | This test is specialised and will only be performed on CSF's when:
CT scan is negative
Four tubes must be collected and the container of the 4th tube must be wrapped in foil (please Label specimen before wrapping in foil) | This test is specialised and will only be performed on CSF's when:
CT scan is negative
Four tubes must be collected and the container of the 4th tube must be wrapped in foil (please Label specimen before wrapping in foil)
A white top tube must be collected for simulataneous measurement of serum Bilirubin and serum Protein (or add on to existing bloods within 6 hours of CSF collection). |
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