Time points for collection: 7 days after peg-asparaginase and just prior to the next dose after E. Coli asparaginase.
Ensure that the request slip mentions the type of asparaginase preparation and time of sample collection in relation to the last dose.
One tube, each EDTA and serum samples
Fill tube completely, minimum 2 ml of blood.
Invert the tube not less than five times.
Centrifuge within two hours at 2200 rpm × g for 10 min.
Use pipette to transfer all of the plasma/serum into a cryovial.
Freeze immediately at –20?C or –80?C until shipment/testing.
Send to Heather Aumann in Immunology at the Alfred
Alfred perform the test weekly (Thursday) | 
