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Dynamic test requiring blood draws at 30 minute interals for 2 hours (5 samples total, for both growth hormone and glucose.
75g glucose (in 300mL solution) administered orally immediately after the first blood draw.
Timepoints:
- 5 min: First collection: Glucose, GH and IGF-1
0 min: 75g glucose adminsitered (as per usual OGTT)
+ 30 min: Second collection: GH and Glucose.
+ 60 min: Third collection: GH and Glucose.
+ 90 min: Fourth collection: GH and Glucose.
+ 120 min: Fifth collection: GH and Glucose.
Keep samples with patient until end of test, then send to lab together. Growth Hormone is stable for 8 hours at room temperature.
Send samples together at end of test.
2mL per Serum sample, 2mL per flox sample.
For diagnostic purposes, negative test if GH < 1.0 at any timepoint.
For monitoring purposes, neg test if GH < 0.5 at any time point.
Spine separate and freeze GH, Analyse glucose in house.
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