Pathology

Test & Collection Guide

FISH for C-MYC, bcl-2 and bcl-6 on paraffin embedded tissue

Ordering information:

Cerner Orderable: Histology Specimen, free text FISH. Or paper request as an add on test to an existing specimen.

All referals for paraffin-embedded tissue FISH must be directly with the laboratory (03 9231-4154) prior to sending specimens.

Alternate names:

Tissue block FISH

Laboratory:

Referred Test

Test Code:

HFISH

Specimen types:

Tissue

Container types:

See Instructions

Collection Instructions:

A request slip indicating the clinical notes, prefered FISH probe if known and tissue type.

The follwing 2 micron sections of tissue on positively charged slides (as per immunohistochemistry), clearly labelled with patient name and section thickness must be sent:

a. 4 patient slides per probe. NOTE: For MYC testing a minimum of 6 slides are required. For >2 probes eg. "double hit" samples a minimum of 9 slides are required.

b. 1 H&E stained slide of the section intended for FISH with a marker indicating the optimal region for FISH - ie. the region with the most concentrated area of tumour.

See attached referal instruction sheet: https://pathology.easternhealth.org.au/handbook/downloads/anatomical-pathology/FISH_VCGS_instructions_2018.pdf

Notes:

Section thickness should be labelled on all slides. Sections greater than 2 microns will not be accepted for processing. Decalcified sections are not suitable for this FISH test.

Results will be available within 2-4 weeks of receipt of specimen. If a result is required urgently, please discuss directly with the lab.

Result Turnaround Time:

7-10 days

Laboratory Use Only

Transport Instructions:

Please deliver all specimes to:

Victorian Cancer Cytogenetics Service

St Vincent's Hospital Melbourne

Second Floor, Building A Destination A26

41 Victoria Parade, Fitzroy, VIC, 3065.

T: 9231 4154 F:9231 4155

[email protected] or [email protected]

External Laboratory:

St Vincents Hospital

External Laboratory details...