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Test & Collection Guide

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PLEASE NOTE: Recent changes have been made to this Test


 Recent changes for Creutzfeldt-Jakob Disease

Recent changes for Creutzfeldt-Jakob Disease

Date
Field
Changed From
Changed To
1st September 2023
Collection & Request Instructions

Requests for Alzheimer's disease - CSF must be collected in a seperate blue capped PP tube, available from Pathology Specimen Reception (CSR).

Processing Instructions

DO NOT CENTRIFUGE SPECIMEN

This test requires authorisation by the Neurology Registrar or Neurology Consultant.

CSF Specimens are unsuitable for testing if macroscopically haemorrhagic, xanthochromic, have red blood cell counts < 500/uL, have white cell counts < 10/uL

DO NOT CENTRIFUGE SPECIMEN

This test requires authorisation by the Neurology Registrar or Neurology Consultant.

CSF Specimens are unsuitable for testing if macroscopically haemorrhagic, xanthochromic. Cell count must have red blood cell counts < 500/uL, and white cell counts < 10/uL

Notes

This test requires authorisation by the Neurology Registrar or Neurology Consultant.

In addition, please refer to Eastern Health Infection Control CJD procedure and contact Infection Control prior to performing the Lumbar Puncture

Test is run weekly. Samples received by 4 p.m. Friday will have the result issued by the end of the following week.

 

CSF : Tests for 14-3-3 CSF protein

At least 1 mL of CSF is required in a separate (4th), unopened tube.

Double bag specimen. Must be frozen and transported on dry ice.

Blood : Test for PRNP (Prion protein gene)

5 mLs in EDTA tube

This test requires authorisation by the Neurology Registrar or Neurology Consultant.

In addition, please refer to Eastern Health Infection Control CJD procedure and contact Infection Control prior to performing the Lumbar Puncture

Test is run weekly. Samples received by 4 p.m. Friday will have the result issued by the end of the following week.

 

CSF : Tests for 14-3-3 CSF protein

At least 1 mL of CSF is required in a separate (4th), unopened tube.

Double bag specimen. Note: sample is no longer required to be sent frozen.

Blood : Test for PRNP (Prion protein gene)

5 mLs in EDTA tube

Creutzfeldt-Jakob Disease

Ordering information:

Domestic samples incur a non-rebatable fee of $ AUD150.00

Alternate names:
14-3-3 CJD, 14-3-3 CSF protein, CJD, CSF - CJD, Protein 14-3-3
Laboratory:
Referred Test
Test Code:
CJD
Specimen types:
Cerebrospinal Fluid
Blood
Container types:
CSF
EDTA
CSF
Collection Instructions:

Requests for Alzheimer's disease - CSF must be collected in a seperate blue capped PP tube, available from Pathology Specimen Reception (CSR).

Minimum Adult Volume:
1 mL
Notes:

This test requires authorisation by the Neurology Registrar or Neurology Consultant.

In addition, please refer to Eastern Health Infection Control CJD procedure and contact Infection Control prior to performing the Lumbar Puncture

Test is run weekly. Samples received by 4 p.m. Friday will have the result issued by the end of the following week.

 

CSF : Tests for 14-3-3 CSF protein

At least 1 mL of CSF is required in a separate (4th), unopened tube.

Double bag specimen. Note: sample is no longer required to be sent frozen.

Blood : Test for PRNP (Prion protein gene)

5 mLs in EDTA tube

Frequency:
Weekly
Result Turnaround Time:
7 days
Laboratory Use Only
Storage Instructions:

DO NOT CENTRIFUGE SPECIMEN

This test requires authorisation by the Neurology Registrar or Neurology Consultant.

CSF Specimens are unsuitable for testing if macroscopically haemorrhagic, xanthochromic. Cell count must have red blood cell counts < 500/uL, and white cell counts < 10/uL

Laboratory Notes:

Link to request form Updated 14.6.18

https://www.florey.edu.au/uploads/content/CJD-FO-008_CSF_Specimen_Data_Sheet_with_14-3-3_and_Tau.pdf

NOTIFY REGISTRY THAT SAMPLE IS BEING SENT.

Transport on dry ice.

CSF : Test is run weekly. Samples received by 4 p.m. Friday will have the result issued by the end of the following week.

 Blood : Test for PRNP (Prion protein gene)

5 mLs in EDTA tube

Send to

The Australian National CJD Registry

Dept. of Pathology, Faculty of Medicine

The University of Melbourne

Melbourne Brain Centre (Kenneth Myer Building)

Cnr Genetics Lane & Royal Pde

Gate 11, Rear Loading Dock

Parkville , Victoria 3052

Phone 8344 1949

Fax 8349 5105

 

External Laboratory:
Australian National CJD Registry
 External Laboratory details...

Pathology Handbook

Test & Collection Guide

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