Pathology

To order lymphocyte or T cell subsets in EMR type "FLOW CYTOMETRY" in the search window and select the relevant panel required based on the test required.

Testing may be indicated in immunodeficiency or as part of monitoring of drug therapy.

Clinical data is valuable for Flow Cytometry staff to assist interpretation.

T subsets include: CD3 count, CD4 count, CD8 count, CD4:CD8 ratio

Lymphocyte subsets include: CD3 count, CD4 count, CD8 count, CD4:CD8 ratio, CD19 count & CD56 count

PLEASE NOTE: Flow Cytometry is a specialised procedure that is performed only during normal business hours.  If there is an extremely urgent need to perform this test outside of these hours, the laboratory must be contacted before the sample is collected. 

It is also imperative that the reason(s) for requesting flow cytometry are clearly indicated on the request form, including contact details for the requesting doctor.

Sample store at ROOM TEMPERATURE. Samples are not stable for more than 24 hours. Hence they must be received by the Flow Cytometry lab at Box Hill within normal business hours Monday to Friday.

Cut-off for outpatient sample collection on Fridays is at 11.30 AM and MUST reach the laboratory before 2.00PM.

Flow cytometric analysis of cells stained by immunofluorescence with a range of antibodies against lymphoid and myeloid markers.

The panel of markers used will be appropriate to the panel selected and the clinical comments supplied, or as arranged by prior consultation.

Samples must be marked 'URGENT', must reach the laboratory as soon as possible. 

All out-of-hours testing requires notification.

Time and date of collection must be recorded on request form.

Whole blood - 1 x EDTA sample, do not spin. Store at room temperature. An FBE must also be requested, and needs a separate EDTA sample.

Prefer EDTA sample.

Do not spin, store at room temperature.