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PLEASE NOTE: Recent changes have been made to this Test


 Recent changes for Cerebrospinal Fluid - Biochemistry

Recent changes for Cerebrospinal Fluid - Biochemistry

Date
Field
Changed From
Changed To
14th August 2020
Collection & Request Instructions

Please note : to avoid sample deterioration, DO NOT send CSF specimens via the vacuum chute system.

Depending on the tests requested, 3-4 separate tubes will need to be provided.  Note that the tubes are numbered so that order of collection can be identified – this order should be carefully followed, so that the optimal sample can be used for the requested tests.  It is also important to consider the tests that are required, as this will determine how much CSF is needed in each tube.  See the matrix below for details.

TUBE 1 is used for Cytology.

TUBE 2 is used for Glucose, Protein and Flow Cytometry.

(centrifuged in the lab – the supernatant is used for Biochemistry analysis (glucose, protein) and the cellular pellet will be used for flow cytometry if required).

TUBE 3 is for Microbiological analysis (cell count, Gram stain and culture) and any further infectious testing (e.g. viral PCR, mycobacterial PCR and culture, cryptococcal antigen).

TUBE 4 will be required only for;

         (a) Creutzfeld-Jacob Disease (CJD) testing OR

(b) Xanthochromia analysis (wrap the tube in foil to prevent    sample deterioration due to light).

 

TESTS REQUIRED

MINIMUM REQUIRED VOLUME (mL)

TUBE 1

TUBE 2

TUBE 3

TUBE 4

CYTOLOGY. CHEMISTRY, MICRO/CULTURE

1.0-2.0

1.0

1.0-2.0

Nil

ADD FLOW CYTOMETRY

 

add 5.0-7.0

 

 

ADD OTHER INFECTIOUS MARKERS

 

 

add 2.0- 5.0-

 

ADD MYCOBACTERIAL CULTURE

 

 

Add 10.00

 

ADD CJD

 

 

 

1.0

ADD XANTHOCHROMIA

 

 

 

1.0 (foil)

 

Note, the ability to add further tests to a previously collected sample is dependent upon the volume provided.  Also, many tests (flow cytometry, cytology) can only be performed on fresh specimens.

 

Other specialised testing (e.g. oligoclonal bands) will be performed using the supplied samples.

 

Xanthochromia and CJD analysis cannot be performed on a blood-stained sample.

Please note : to avoid sample deterioration, DO NOT send CSF specimens via the vacuum chute system.

Depending on the tests requested, 3-4 separate tubes will need to be provided.  Note that the tubes are numbered so that order of collection can be identified – this order should be carefully followed, so that the optimal sample can be used for the requested tests.  It is also important to consider the tests that are required, as this will determine how much CSF is needed in each tube.  See the matrix below for details.

TUBE 1 is used for Cytology.

TUBE 2 is used for Glucose, Protein and Flow Cytometry.

(centrifuged in the lab – the supernatant is used for Biochemistry analysis (glucose, protein) and the cellular pellet will be used for flow cytometry if required).

TUBE 3 is for Microbiological analysis (cell count, Gram stain and culture) and any further infectious testing (e.g. viral PCR, mycobacterial PCR and culture, cryptococcal antigen).

TUBE 4 will be required only for;

         (a) Creutzfeld-Jacob Disease (CJD) testing OR

(b) Xanthochromia analysis (wrap the tube in foil to prevent    sample deterioration due to light).

 

TESTS REQUIRED

MINIMUM REQUIRED VOLUME (mL)

TUBE 1

TUBE 2

TUBE 3

TUBE 4

CYTOLOGY. CHEMISTRY, MICRO/CULTURE

1.0-2.0

1.0

1.0-2.0

Nil

ADD FLOW CYTOMETRY

 

add 5.0-7.0

 

 

ADD OTHER INFECTIOUS MARKERS

 

 

add 2.0- 5.0-

 

ADD MYCOBACTERIAL CULTURE

 

 

Add 10.00

 

ADD CJD

 

 

 

1.0

ADD XANTHOCHROMIA

 

 

 

1.0 (foil)

 

Note, the ability to add further tests to a previously collected sample is dependent upon the volume provided.  Also, many tests (flow cytometry, cytology) can only be performed on fresh specimens.

 

Other specialised testing (e.g. oligoclonal bands) will be performed using the supplied samples.

 

Xanthochromia and CJD analysis cannot be performed on a blood-stained sample.

CSF Information - Laboratory Processing

14th August 2020
Collection & Request Instructions

Please note : to avoid sample deterioration, DO NOT send CSF specimens via the vacuum chute system.

If additional tests required eg. CSF oligoclonal bands, a minimum of 1.0 ml is required.

Collect CSF into three separate, sterile, screw-capped tubes provided (labelled 1,2 & 3)

Tube 1 is for Biochemistry (for Protein and Glucose) 0.5ml (10 drops) required 

Tube 2 for Cytology 1.0ml (20 drops)  and/or Flow Cytometry (6ml)

For Flow Cytometry a minimum of 6ml is required  in Tube 2

For Flow Cytometry and Cytology a minimum of 7ml is required in Tube 2     

Tube 3 for Microbiology upto 10ml (Full tube)

If querying CJD the laboratory requires a 4th unopened tube containing at least 1ml of non-bloodstained CSF.

Xanthochromia investigation requires a 4th tube containing 1ml of CSF .

Please note : to avoid sample deterioration, DO NOT send CSF specimens via the vacuum chute system.

Depending on the tests requested, 3-4 separate tubes will need to be provided.  Note that the tubes are numbered so that order of collection can be identified – this order should be carefully followed, so that the optimal sample can be used for the requested tests.  It is also important to consider the tests that are required, as this will determine how much CSF is needed in each tube.  See the matrix below for details.

TUBE 1 is used for Cytology.

TUBE 2 is used for Glucose, Protein and Flow Cytometry.

(centrifuged in the lab – the supernatant is used for Biochemistry analysis (glucose, protein) and the cellular pellet will be used for flow cytometry if required).

TUBE 3 is for Microbiological analysis (cell count, Gram stain and culture) and any further infectious testing (e.g. viral PCR, mycobacterial PCR and culture, cryptococcal antigen).

TUBE 4 will be required only for;

         (a) Creutzfeld-Jacob Disease (CJD) testing OR

(b) Xanthochromia analysis (wrap the tube in foil to prevent    sample deterioration due to light).

 

TESTS REQUIRED

MINIMUM REQUIRED VOLUME (mL)

TUBE 1

TUBE 2

TUBE 3

TUBE 4

CYTOLOGY. CHEMISTRY, MICRO/CULTURE

1.0-2.0

1.0

1.0-2.0

Nil

ADD FLOW CYTOMETRY

 

add 5.0-7.0

 

 

ADD OTHER INFECTIOUS MARKERS

 

 

add 2.0- 5.0-

 

ADD MYCOBACTERIAL CULTURE

 

 

Add 10.00

 

ADD CJD

 

 

 

1.0

ADD XANTHOCHROMIA

 

 

 

1.0 (foil)

 

Note, the ability to add further tests to a previously collected sample is dependent upon the volume provided.  Also, many tests (flow cytometry, cytology) can only be performed on fresh specimens.

 

Other specialised testing (e.g. oligoclonal bands) will be performed using the supplied samples.

 

Xanthochromia and CJD analysis cannot be performed on a blood-stained sample.

Cerebrospinal Fluid - Biochemistry

Alternate names:
CSF - Biochemistry
Laboratory:
Biochemistry
Test Code:
CSFB
Specimen types:
Cerebrospinal Fluid
Container types:
CSFCSF
Collection Instructions:

Please note : to avoid sample deterioration, DO NOT send CSF specimens via the vacuum chute system.

Depending on the tests requested, 3-4 separate tubes will need to be provided.  Note that the tubes are numbered so that order of collection can be identified – this order should be carefully followed, so that the optimal sample can be used for the requested tests.  It is also important to consider the tests that are required, as this will determine how much CSF is needed in each tube.  See the matrix below for details.

TUBE 1 is used for Cytology.

TUBE 2 is used for Glucose, Protein and Flow Cytometry.

(centrifuged in the lab – the supernatant is used for Biochemistry analysis (glucose, protein) and the cellular pellet will be used for flow cytometry if required).

TUBE 3 is for Microbiological analysis (cell count, Gram stain and culture) and any further infectious testing (e.g. viral PCR, mycobacterial PCR and culture, cryptococcal antigen).

TUBE 4 will be required only for;

         (a) Creutzfeld-Jacob Disease (CJD) testing OR

(b) Xanthochromia analysis (wrap the tube in foil to prevent    sample deterioration due to light).

 

TESTS REQUIRED

MINIMUM REQUIRED VOLUME (mL)

TUBE 1

TUBE 2

TUBE 3

TUBE 4

CYTOLOGY. CHEMISTRY, MICRO/CULTURE

1.0-2.0

1.0

1.0-2.0

Nil

ADD FLOW CYTOMETRY

 

add 5.0-7.0

 

 

ADD OTHER INFECTIOUS MARKERS

 

 

add 2.0- 5.0-

 

ADD MYCOBACTERIAL CULTURE

 

 

Add 10.00

 

ADD CJD

 

 

 

1.0

ADD XANTHOCHROMIA

 

 

 

1.0 (foil)

 

Note, the ability to add further tests to a previously collected sample is dependent upon the volume provided.  Also, many tests (flow cytometry, cytology) can only be performed on fresh specimens.

 

Other specialised testing (e.g. oligoclonal bands) will be performed using the supplied samples.

 

Xanthochromia and CJD analysis cannot be performed on a blood-stained sample.

CSF Information - Laboratory Processing

Notes:

Glucose and Protein are determined routinely

Glucose result should be compared with plasma glucose taken at the same time

Results on blood-stained CSF are unreliable

Testing for oligoclonal bands performed on request (serum must also be provided) .

Frequency:
Daily
Result Turnaround Time:
4 hours
Reference Interval:

 

Glucose 2.8 - 4.4 mmol/L
Protein 0.15 - 0.45 g/L
Laboratory Use Only
Laboratory Notes:

CSF sample processing:

If Cytology/Flow Cytometry requested  : Tube 1-Biochemistry, Tube 2- Cytology/Flow Cytometry, Tube 3-Microbiology

If Cytology/Flow Cytometry not requested: Tube 1 and 3-Microbiology, Tube 2-Biochemistry