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Test & Collection Guide

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PLEASE NOTE: Recent changes have been made to this Test


 Recent changes for Respiratory Virus PCR

Recent changes for Respiratory Virus PCR

Date
Field
Changed From
Changed To
14th February 2023
Method

The following specimens will be referred for testing-

Sputum, Bronchial Washings, Bronchoalveolar Lavages - VIDRL

Swab specimens for Respiratory Multiplex PCR will be referred to Monash Molecular Laboratory

 

The following specimens will be referred for testing-

Sputum, Bronchial Washings, Bronchoalveolar Lavages - VIDRL

As of Feb 2023 swab specimens for Respiratory Multiplex PCR will be tested in-house.

 

31st August 2022
Preferred Specimen Type

Nasopharyngeal swab
Nasopharyngeal Aspirate

Nasopharyngeal swab
NTS - nose & throat swab
Nasopharyngeal Aspirate

Optional Specimen Type

Sputum
Bronchial Washings
Broncho-alveolar Lavage

Preferred Container Type

Viral Transport Media- Nasopharyngeal
Respiratory aspiration pot

Viral Transport Media- Nasopharyngeal
Respiratory aspiration pot
respiratory PCR requirements

Collection & Request Instructions

Please note these swabs are available from stores.

Search for: VIRAL TRANSPORT MEDIA - NASOPHARYNGEAL

Pathology do not keep a stock of these swabs. Emergency departments and wards should manage a supply of these swabs according to demand.

 

 

Please note these swabs are available from stores.

Pathology do not keep a stock of these swabs. Emergency departments and wards should manage a supply of these swabs according to demand.

 

 

Method

All tests for respiratory virus PCR are considered urgent and must be delivered to the laboratory ASAP.

ICU/Oncology patients testing negative for Flu A/B and RSV  will be referred to Monash for respiratory virus panel. This includes Influenza A virus, Influenza B virus, Respiratory Syncitial Virus, Picornavirus (RV/EV), Parainfluenza viruses 1,2 and 3, Adenovirus, Human Metapneumovirus, Bordetella pertussis, Bordetella parapertussis, Mycoplasma pneumoniae, Legionella species, Chlamydiaceae.

The following specimens will be referred for testing-

Sputum, Bronchial Washings, Bronchoalveolar Lavages

Specimens for Respiratory Multiplex PCR will be referred to Monash Molecular Laboratory

Contact Monash Pathology 9594 4532 or if no answer try Bacteriology 9594 4565 as there is someone rostered 24/7

 

The following specimens will be referred for testing-

Sputum, Bronchial Washings, Bronchoalveolar Lavages - VIDRL

Swab specimens for Respiratory Multiplex PCR will be referred to Monash Molecular Laboratory

 

Notes

This PCR will detect and identify: Influenza A virus, Influenza B virus and Respiratory syncytial virus (RSV).

Transport at room temperature to the Laboratory

Testing is performed at Box Hill, Angliss, and Maroondah.

Testing  Lab

Testing hours

TAT (from receipt in lab)

All sites

 

Available 24/7 

1 hr

Samples received after hours may have a longer TAT due to other urgent critical pathology testing occurring.

This PCR will detect and identify: Influenza A virus, Influenza B virus and Respiratory syncytial virus (RSV).

Transport at room temperature to the Laboratory

Testing is performed at Box Hill, Angliss, and Maroondah.

Testing  Lab

Testing hours

TAT (from receipt in lab)

All sites

 

Available 24/7 

6-12 hrs routine

2 hours (rapid approval form required)

DO NOT send samples in the Pathology Chute

For Rapid testing from Emergency Department - swab must be accompanied by signed Rapid Test Authorisation Form.

DO NOT call the laboratory for results/enquiries. Inpatient tests will be prioritised and results available on EMR ASAP.

Sputum samples are referred to an external laboratory - results will be delayed.

Respiratory Virus PCR

Ordering information:

 

NB: requests for COVID-19 PCR will automatically have this test added.

SPECIMEN REQUIREMENTS

Collect a combined nose & throat swab, or a nasopharyngeal swab.

Swabs MUST be collected using UTM Viral transport media (green top or red top tubes)

EMR orders MUST specify collection site

Urgent testing is available at each site but sample must be accompanied by a Rapid Approval Request form (available via Objectify Policy 244). Routine testing is performed at Box Hill Microbiology Dept.

Sputum, Bronchial Washings, Bronchoalveolar Lavages are referred to an external laboratory for testing.

All other requests for multiplex PCR require approval from the Clinical Microbiologist

 

 

 

 

 

 

Alternate names:
Influenza NAD, Respiratory Viral NAD, RSV NAD
Laboratory:
Microbiology
Test Code:
RESPCR
Specimen types:
Nasopharyngeal swab
NTS - nose & throat swab
Nasopharyngeal Aspirate
Sputum
Bronchial Washings
Broncho-alveolar Lavage
Container types:
Viral Transport Media- Nasopharyngeal
Respiratory aspiration pot
respiratory PCR requirements
Viral Transport Media- Nasopharyngeal
Collection Instructions:

Please note these swabs are available from stores.

Pathology do not keep a stock of these swabs. Emergency departments and wards should manage a supply of these swabs according to demand.

 

 

Notes:

This PCR will detect and identify: Influenza A virus, Influenza B virus and Respiratory syncytial virus (RSV).

Transport at room temperature to the Laboratory

Testing is performed at Box Hill, Angliss, and Maroondah.

Testing  Lab

Testing hours

TAT (from receipt in lab)

All sites

 

Available 24/7 

6-12 hrs routine

2 hours (rapid approval form required)

DO NOT send samples in the Pathology Chute

For Rapid testing from Emergency Department - swab must be accompanied by signed Rapid Test Authorisation Form.

DO NOT call the laboratory for results/enquiries. Inpatient tests will be prioritised and results available on EMR ASAP.

Sputum samples are referred to an external laboratory - results will be delayed.

Frequency:
Daily
Result Turnaround Time:
6-12 hours
Laboratory Use Only
Laboratory Notes:

The following specimens will be referred for testing-

Sputum, Bronchial Washings, Bronchoalveolar Lavages - VIDRL

As of Feb 2023 swab specimens for Respiratory Multiplex PCR will be tested in-house.

 

Pathology Handbook

Test & Collection Guide

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