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Notification of anticoagulant therapy is essential
One (1x) Sodium citrate tubes is required. Do not use 1 ml citrate tubes.
Filled to label marker and mixed by inversion
The patient must not be receiving heparin
Unspun sample is stable for up to 4 hours and must be transport to laboratory immediately. (Sample must be double spun, aliquoted and frozen within 4 hours from time of collection).
Tests performed:
INR, APTT, APTT Mix, Silica Clotting Time and Dilute Russell's Viper venom time (LA screen/Confirm).
LA screen <1.2 Lupus anticoagulant not detected.
LA Confirm <1.2 Lupus anticoagulant not detected
1.2 - 1.5 L.A. weakly present
1.5 - 2.0 L.A. moderately present.
>2.0 L.A. strongly present
Samples can be transported frozen to the laboratory if the facilities are available to double spin and aliquot these samples. This has to be performed within 4 hours of collection.
**Sample must be centrifuged within 4 hours of collection**
Centrifuge the two 3.5 mL citrate tubes (and transfer the plasma to a labeled 4 mL plastic tube and re-centrifuge at room temperature (do not use the refrigerated centrifuge). One sample is acceptable but 2 samples are preferable for Lupus testing.
The double centrifuged plasma is then transferred into new labelled tubes.
Double spun samples are then frozen in the -20'C freezer.
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