Test & Collection Guide

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Vitamin D Metabolites

Ordering information:

Effective 15/12/2015, Vitamin D testing can only be requested for a prescribed list of clinical situations (see list below).  The request form MUST include the clinical indication for the test.  In the absence of this information, testing cannot be performed.



Signs or symptoms of osteoporosis or osteomalacia

 Increased alkaline phosphatase and otherwise normal LFT's

Hyperparathyroidism, hypo- or hypercalcaemia, or hypophosphataemia;

Malabsorption conditions

Deeply pigmented skin, or chronic and severe lack of sun exposure for cultural, medical, occupational or  residential reasons

Taking medication known to decrease 25OH-D levels (for example, anticonvulsants)

Chronic renal failure or renal transplant recipient

<16 years of age with signs or symptoms of rickets

An infant whose mother has established vitamin D deficiency

      Exclusively breastfed baby with at least one other risk factor mentioned in this list

Has a sibling who is less than 16 years of age and has vitamin D deficiency


Alternate names:
25 di-Hydroxy Vitamin D3, 25(OH) Vitamin D, Vit D
Specimen types:
Container types:
Plain Tube with gelPlain Tube with gel
Collection Instructions:
Transport specimen at Room Temperature to lab

Store and transport serum at 4 o C

Note: Vitamin D can be added on to the specimens up to 48 hours after the collection (at room temperature) or up to 7 days for specimen stored at 4 o C.


Minimum Adult Volume:
1 mL


  Specimens for vitamin assays must be taken before dietary changes are implemented  

Please note: Change in Vitamin D assay as of 22nd June 2011.  The new method is a Total VItamin D method which measures both Vitamin D2 and D3.

Result Turnaround Time:
5 hours
Reference Interval:

> 75 nmol/L


 Vitamin D levels >75 nmol/L are recommended to lower fracture risk. Source:

Osteoporos Int 2005; 16(7):713-6.

 Vitamin D levels < 50 indicate deficiency.


25 Hydroxy Vitamin D3 is the appropriate metabolite to asess in suspected deficiency states.



Occasionally 1,25 di-Hydroxy Vitamin D3 is indicated, but discussion with Chemical Pathologist is required prior to testing by an external provider.



Enquiries - Phone Core Laboratory Box Hill Hospital; 9895 3473


Laboratory Use Only
Storage Instructions:

1.25 di Hydroxyvitamin D if required is performed at Austin Pathology and requires a minimum volume of 0.6 mL of serum - Frozen asap