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PLEASE NOTE: Recent changes have been made to this Test


 Recent changes for Leucocyte Immunophenotyping

Recent changes for Leucocyte Immunophenotyping

Date
Field
Changed From
Changed To
15th October 2021
Notes

Samples must be marked 'URGENT', must reach the laboratory as soon as possible. 

All out-of-hours testing requires notification.

Time and date of collection must be recorded on request form.

Whole blood - 1 x EDTA sample, do not spin. Store at room temperature. An FBE must also be requested,  and needs a separate EDTA sample.

Bone marrow aspirate - Sample into  EDTA tube

TissueFresh (if to be shared with other departments for other tests eg. Histopathology or Microbiology) or RPMI fluid (especially if specimen is taken out of hours). This fluid is obtainable from: BH Lab 9895 3884, MH Lab: 9871 3572 or AH Lab: 9764 6136.

CSF/other fluids

Depending on the tests requested, 3-4 separate tubes will need to be provided. Note that the tubes are numbered so that order of collection can be identified – this order should be carefully followed, so that the optimal sample can be used for the requested tests. It is also important to consider the tests that are required, as this will determine how much CSF is needed in each tube. See the matrix below for details.

TUBE 1 is used for Biochemistry and Flow Cytometry. (Centrifuged in the lab – the supernatant is used for Biochemistry analysis (glucose, protein) and the cellular pellet will be used for flow cytometry if required). If tube 3 is bloodstained, take an aliquot for microbiology.

TUBE 2 is used for Cytology

TUBE 3 is for Microbiological analysis (cell count, Gram stain and culture) and any further infectious testing (e.g. viral PCR, mycobacterial PCR and culture, cryptococcal antigen).

TUBE 4 will be required only for;

(a) Creutzfeld-Jacob Disease (CJD) testing OR

(b) Xanthochromia analysis (wrap the tube in foil to prevent sample deterioration due to light).

 

TESTS REQUIRED

MINIMUM REQUIRED VOLUME (mL)

TUBE 1

TUBE 2

TUBE 3

TUBE 4

CHEMISTRY

1.0

 

 

 

ADD FLOW CYTOMETRY

Minimum 5.0-7.0

 

 

 

CYTOLOGY

 

1.0-3.0

 

 

MICROBIOLOGY

 

 

1.0-2.0

 

ADD OTHER INFECTIOUS MARKERS

 

 

add 2.0- 5.0-

 

ADD MYCOBACTERIAL CULTURE

 

 

Add 6.0-10.00

 

ADD CJD

 

 

 

1.0

ADD XANTHOCHROMIA

 

 

 

1.0 (foil)

 

Note, the ability to add further tests to a previously collected sample is dependent upon the volume provided. Also, many tests (flow cytometry, cytology) can only be performed on fresh specimens.

 

Other specialised testing (e.g. oligoclonal bands) will be performed using the supplied samples.

Xanthochromia and CJD analysis cannot be performed on a blood-stained sample

Samples must be marked 'URGENT', must reach the laboratory as soon as possible. 

All out-of-hours testing requires notification.

Time and date of collection must be recorded on request form.

Whole blood - 1 x EDTA sample, do not spin. Store at room temperature. An FBE and BLOOD FILM must also be requested,  and needs a separate EDTA sample.

Bone marrow aspirate - Sample into  EDTA tube

TissueFresh (if to be shared with other departments for other tests eg. Histopathology or Microbiology) or RPMI fluid (especially if specimen is taken out of hours). This fluid is obtainable from: BH Lab 9895 3884, MH Lab: 9871 3572 or AH Lab: 9764 6136.

CSF/other fluids

Depending on the tests requested, 3-4 separate tubes will need to be provided. Note that the tubes are numbered so that order of collection can be identified – this order should be carefully followed, so that the optimal sample can be used for the requested tests. It is also important to consider the tests that are required, as this will determine how much CSF is needed in each tube. See the matrix below for details.

TUBE 1 is used for Biochemistry and Flow Cytometry. (Centrifuged in the lab – the supernatant is used for Biochemistry analysis (glucose, protein) and the cellular pellet will be used for flow cytometry if required). If tube 3 is bloodstained, take an aliquot for microbiology.

TUBE 2 is used for Cytology

TUBE 3 is for Microbiological analysis (cell count, Gram stain and culture) and any further infectious testing (e.g. viral PCR, mycobacterial PCR and culture, cryptococcal antigen).

TUBE 4 will be required only for;

(a) Creutzfeld-Jacob Disease (CJD) testing OR

(b) Xanthochromia analysis (wrap the tube in foil to prevent sample deterioration due to light).

 

TESTS REQUIRED

MINIMUM REQUIRED VOLUME (mL)

TUBE 1

TUBE 2

TUBE 3

TUBE 4

CHEMISTRY

1.0

 

 

 

ADD FLOW CYTOMETRY

Minimum 5.0-7.0

 

 

 

CYTOLOGY

 

1.0-3.0

 

 

MICROBIOLOGY

 

 

1.0-2.0

 

ADD OTHER INFECTIOUS MARKERS

 

 

add 2.0- 5.0-

 

ADD MYCOBACTERIAL CULTURE

 

 

Add 6.0-10.00

 

ADD CJD

 

 

 

1.0

ADD XANTHOCHROMIA

 

 

 

1.0 (foil)

 

Note, the ability to add further tests to a previously collected sample is dependent upon the volume provided. Also, many tests (flow cytometry, cytology) can only be performed on fresh specimens.

 

Other specialised testing (e.g. oligoclonal bands) will be performed using the supplied samples.

Xanthochromia and CJD analysis cannot be performed on a blood-stained sample

14th October 2021
Notes

 

 

Samples must be marked 'URGENT', must reach the laboratory as soon as possible. 

All out-of-hours testing requires notification.

Time and date of collection must be recorded on request form.

Whole blood - 1 x EDTA sample, do not spin. Store at room temperature. An FBE must also be requested,  and needs a separate EDTA sample.

Bone marrow aspirate - Sample into  EDTA tube

TissueFresh (if to be shared with other departments for other tests eg. Histopathology or Microbiology) or RPMI fluid (especially if specimen is taken out of hours). This fluid is obtainable from: BH Lab 9895 3884, MH Lab: 9871 3572 or AH Lab: 9764 6136.

CSF/other fluids - at least 2mL is preferred

Depending on the tests requested, 3-4 separate tubes will need to be provided. Note that the tubes are numbered so that order of collection can be identified – this order should be carefully followed, so that the optimal sample can be used for the requested tests. It is also important to consider the tests that are required, as this will determine how much CSF is needed in each tube. See the matrix below for details.

TUBE 1 is used for Biochemistry and Flow Cytometry. (Centrifuged in the lab – the supernatant is used for Biochemistry analysis (glucose, protein) and the cellular pellet will be used for flow cytometry if required). If tube 3 is bloodstained, take an aliquot for microbiology.

TUBE 2 is used for Cytology

TUBE 3 is for Microbiological analysis (cell count, Gram stain and culture) and any further infectious testing (e.g. viral PCR, mycobacterial PCR and culture, cryptococcal antigen).

TUBE 4 will be required only for;

(a) Creutzfeld-Jacob Disease (CJD) testing OR

(b) Xanthochromia analysis (wrap the tube in foil to prevent sample deterioration due to light).

 

TESTS REQUIRED

MINIMUM REQUIRED VOLUME (mL)

TUBE 1

TUBE 2

TUBE 3

TUBE 4

CHEMISTRY

1.0

 

 

 

ADD FLOW CYTOMETRY

Minimum 5.0-7.0

 

 

 

CYTOLOGY

 

1.0-3.0

 

 

MICROBIOLOGY

 

 

1.0-2.0

 

ADD OTHER INFECTIOUS MARKERS

 

 

add 2.0- 5.0-

 

ADD MYCOBACTERIAL CULTURE

 

 

Add 6.0-10.00

 

ADD CJD

 

 

 

1.0

ADD XANTHOCHROMIA

 

 

 

1.0 (foil)

 

Note, the ability to add further tests to a previously collected sample is dependent upon the volume provided. Also, many tests (flow cytometry, cytology) can only be performed on fresh specimens.

 

Other specialised testing (e.g. oligoclonal bands) will be performed using the supplied samples.

Xanthochromia and CJD analysis cannot be performed on a blood-stained sample

 

 

Samples must be marked 'URGENT', must reach the laboratory as soon as possible. 

All out-of-hours testing requires notification.

Time and date of collection must be recorded on request form.

Whole blood - 1 x EDTA sample, do not spin. Store at room temperature. An FBE must also be requested,  and needs a separate EDTA sample.

Bone marrow aspirate - Sample into  EDTA tube

TissueFresh (if to be shared with other departments for other tests eg. Histopathology or Microbiology) or RPMI fluid (especially if specimen is taken out of hours). This fluid is obtainable from: BH Lab 9895 3884, MH Lab: 9871 3572 or AH Lab: 9764 6136.

CSF/other fluids

Depending on the tests requested, 3-4 separate tubes will need to be provided. Note that the tubes are numbered so that order of collection can be identified – this order should be carefully followed, so that the optimal sample can be used for the requested tests. It is also important to consider the tests that are required, as this will determine how much CSF is needed in each tube. See the matrix below for details.

TUBE 1 is used for Biochemistry and Flow Cytometry. (Centrifuged in the lab – the supernatant is used for Biochemistry analysis (glucose, protein) and the cellular pellet will be used for flow cytometry if required). If tube 3 is bloodstained, take an aliquot for microbiology.

TUBE 2 is used for Cytology

TUBE 3 is for Microbiological analysis (cell count, Gram stain and culture) and any further infectious testing (e.g. viral PCR, mycobacterial PCR and culture, cryptococcal antigen).

TUBE 4 will be required only for;

(a) Creutzfeld-Jacob Disease (CJD) testing OR

(b) Xanthochromia analysis (wrap the tube in foil to prevent sample deterioration due to light).

 

TESTS REQUIRED

MINIMUM REQUIRED VOLUME (mL)

TUBE 1

TUBE 2

TUBE 3

TUBE 4

CHEMISTRY

1.0

 

 

 

ADD FLOW CYTOMETRY

Minimum 5.0-7.0

 

 

 

CYTOLOGY

 

1.0-3.0

 

 

MICROBIOLOGY

 

 

1.0-2.0

 

ADD OTHER INFECTIOUS MARKERS

 

 

add 2.0- 5.0-

 

ADD MYCOBACTERIAL CULTURE

 

 

Add 6.0-10.00

 

ADD CJD

 

 

 

1.0

ADD XANTHOCHROMIA

 

 

 

1.0 (foil)

 

Note, the ability to add further tests to a previously collected sample is dependent upon the volume provided. Also, many tests (flow cytometry, cytology) can only be performed on fresh specimens.

 

Other specialised testing (e.g. oligoclonal bands) will be performed using the supplied samples.

Xanthochromia and CJD analysis cannot be performed on a blood-stained sample

14th October 2021
Alternative names

CD4/CD8 Counts, Cell Surface Markers, FACS Analysis, Flow Cytometry

Cell Surface Markers, FACS Analysis, Flow Cytometry, Immunophenotyping

Request Group

FLOW

LYMP, LEUK

Collection & Request Instructions

Sample store at Room temperature. Samples are not stable for more than 24 hours. Hence they must be received by the Flow Cytometry lab at Box Hill within normal business hours Monday to Friday.

Cutoff for outpatient sample collection on Fridays is at 11.30 AM and MUST reach the laboratory before 4.00PM.

Flow cytometric analysis of cells stained by immunofluorescence with a range of antibodies against lymphoid and myeloid markers.

The panel of markers used will be appropriate to the clinical comments supplied , or as arranged by prior consultation.

Sample store at Room temperature. Samples are not stable for more than 24 hours. Hence they must be received by the Flow Cytometry lab at Box Hill within normal business hours Monday to Friday.

Cut-off for outpatient sample collection on Fridays is at 11.30 AM and MUST reach the laboratory before 4.00PM.

Flow cytometric analysis of cells stained by immunofluorescence with a range of antibodies against lymphoid and myeloid markers.

The panel of markers used will be appropriate to the panel selected and the clinical comments supplied , or as arranged by prior consultation

Notes

 

 

Samples must be marked 'URGENT', must reach the laboratory as soon as possible. 

All out-of-hours testing requires notification.

Time and date of collection must be recorded on request form.

Whole blood - 1 x EDTA sample, do not spin. Store at room temperature. An FBE must also be requested, needs a separate EDTA sample.

Bone marrow aspirate - Sample into  EDTA tube

TissueFresh (if to be shared with other departments for other tests eg. Histopathology or Microbiology) or RPMI fluid (especially if specimen is taken out of hours). This fluid is obtainable from: BH Lab 9895 3884, MH Lab: 9871 3572 or AH Lab: 9764 6136.

CSF/other fluids - at least 2mL is preferred

Collect CSF into three separate, sterile, screw-capped tubes provided (labelled 1,2 & 3)

Tube 1 is for Biochemistry (for Protein and Glucose) 0.5ml (10 drops) required 

Tube 2 for Cytology 1.0ml (20 drops)  and/or Flow Cytometry (6ml)

For Flow Cytometry a minimum of 6ml is required  in Tube 2

For Flow Cytometry and Cytology a minimum of 7ml is required in Tube 2     

Tube 3 for Microbiology upto 10ml (Full tube)

If querying CJD the laboratory requires a 4th unopened tube containing at least 1ml of non-bloodstained CSF.

Xanthochromia investigation requires a 4th tube containing 1ml of CSF .

 

 

Samples must be marked 'URGENT', must reach the laboratory as soon as possible. 

All out-of-hours testing requires notification.

Time and date of collection must be recorded on request form.

Whole blood - 1 x EDTA sample, do not spin. Store at room temperature. An FBE must also be requested,  and needs a separate EDTA sample.

Bone marrow aspirate - Sample into  EDTA tube

TissueFresh (if to be shared with other departments for other tests eg. Histopathology or Microbiology) or RPMI fluid (especially if specimen is taken out of hours). This fluid is obtainable from: BH Lab 9895 3884, MH Lab: 9871 3572 or AH Lab: 9764 6136.

CSF/other fluids - at least 2mL is preferred

Depending on the tests requested, 3-4 separate tubes will need to be provided. Note that the tubes are numbered so that order of collection can be identified – this order should be carefully followed, so that the optimal sample can be used for the requested tests. It is also important to consider the tests that are required, as this will determine how much CSF is needed in each tube. See the matrix below for details.

TUBE 1 is used for Biochemistry and Flow Cytometry. (Centrifuged in the lab – the supernatant is used for Biochemistry analysis (glucose, protein) and the cellular pellet will be used for flow cytometry if required). If tube 3 is bloodstained, take an aliquot for microbiology.

TUBE 2 is used for Cytology

TUBE 3 is for Microbiological analysis (cell count, Gram stain and culture) and any further infectious testing (e.g. viral PCR, mycobacterial PCR and culture, cryptococcal antigen).

TUBE 4 will be required only for;

(a) Creutzfeld-Jacob Disease (CJD) testing OR

(b) Xanthochromia analysis (wrap the tube in foil to prevent sample deterioration due to light).

 

TESTS REQUIRED

MINIMUM REQUIRED VOLUME (mL)

TUBE 1

TUBE 2

TUBE 3

TUBE 4

CHEMISTRY

1.0

 

 

 

ADD FLOW CYTOMETRY

Minimum 5.0-7.0

 

 

 

CYTOLOGY

 

1.0-3.0

 

 

MICROBIOLOGY

 

 

1.0-2.0

 

ADD OTHER INFECTIOUS MARKERS

 

 

add 2.0- 5.0-

 

ADD MYCOBACTERIAL CULTURE

 

 

Add 6.0-10.00

 

ADD CJD

 

 

 

1.0

ADD XANTHOCHROMIA

 

 

 

1.0 (foil)

 

Note, the ability to add further tests to a previously collected sample is dependent upon the volume provided. Also, many tests (flow cytometry, cytology) can only be performed on fresh specimens.

 

Other specialised testing (e.g. oligoclonal bands) will be performed using the supplied samples.

Xanthochromia and CJD analysis cannot be performed on a blood-stained sample

20th July 2021
Assay name

Leucocyte Immunophenotyping

T Cell Subsets

Alternative names

CD4/CD8 Counts, Cell Surface Markers, FACS Analysis, Flow Cytometry

CD2, CD3, CD4, CD8

Preferred Container Type

EDTA
Sterile Jar

EDTA
Lithium heparin no gel

Collection & Request Instructions

Sample store at Room temperature. Samples are not stable for more than 24 hours. Hence they must be received by the Flow Cytometry lab at Box Hill within normal business hours Monday to Friday.

Cutoff for outpatient sample collection on Fridays is at 11.30 AM and MUST reach the laboratory before 4.00PM.

Flow cytometric analysis of cells stained by immunofluorescence with a range of antibodies against lymphoid and myeloid markers.

The panel of markers used will be appropriate to the clinical comments supplied , or as arranged by prior consultation.

The samples are not stable for more than 24 hours. Hence all samples must be recieved in the laboratory at Box Hill  Monday to Friday within business hours.

Cutoff for outpatient sample collection on Fridays is at 11.30 AM and MUST reach the laboratory before 4.00PM.

Processing Instructions

Prefer EDTA sample.

Do not spin, store at room temperature.

Notes

 

 

Samples must be marked 'URGENT', must reach the laboratory as soon as possible. 

All out-of-hours testing requires notification.

Time and date of collection must be recorded on request form.

Whole blood - 1 x EDTA sample, do not spin. Store at room temperature. An FBE must also be requested, needs a separate EDTA sample.

Bone marrow aspirate - Sample into  EDTA tube

TissueFresh (if to be shared with other departments for other tests eg. Histopathology or Microbiology) or RPMI fluid (especially if specimen is taken out of hours). This fluid is obtainable from: BH Lab 9895 3884, MH Lab: 9871 3572 or AH Lab: 9764 6136.

CSF/other fluids - at least 2mL is preferred

Collect CSF into three separate, sterile, screw-capped tubes provided (labelled 1,2 & 3)

Tube 1 is for Biochemistry (for Protein and Glucose) 0.5ml (10 drops) required 

Tube 2 for Cytology 1.0ml (20 drops)  and/or Flow Cytometry (6ml)

For Flow Cytometry a minimum of 6ml is required  in Tube 2

For Flow Cytometry and Cytology a minimum of 7ml is required in Tube 2     

Tube 3 for Microbiology upto 10ml (Full tube)

If querying CJD the laboratory requires a 4th unopened tube containing at least 1ml of non-bloodstained CSF.

Xanthochromia investigation requires a 4th tube containing 1ml of CSF .

Used to monitor T-cell numbers

Preferred EDTA sample. Do not spin, store at room temperature.

19th July 2021
Collection & Request Instructions

Sample store at Room temperature. Samples are not stable for more than 24 hours. Hence they must be recieved by the Flow Cytometry lab at Box Hill within normal business hours Monday to Friday.

Cutoff for outpatirnt sample collection on Fridays is at 12:00 PM and MUST reach the laboratory by 4.00PM.

Flow cytometric analysis of cells stained by immunofluorescence with a range of antibodies against lymphoid and myeloid markers.

The panel of markers used will be appropriate to the clinical comments supplied , or as arranged by prior consultation.

Sample store at Room temperature. Samples are not stable for more than 24 hours. Hence they must be received by the Flow Cytometry lab at Box Hill within normal business hours Monday to Friday.

Cutoff for outpatient sample collection on Fridays is at 11.30 AM and MUST reach the laboratory before 4.00PM.

Flow cytometric analysis of cells stained by immunofluorescence with a range of antibodies against lymphoid and myeloid markers.

The panel of markers used will be appropriate to the clinical comments supplied , or as arranged by prior consultation.

19th July 2021
Collection & Request Instructions

Sample store at Room temperature. Samples are not stable for more than 24 hours. Hence they must be recieved by the Flow Cytometry lab at Box Hill within normal business hours Monday to Friday

Flow cytometric analysis of cells stained by immunofluorescence with a range of antibodies against lymphoid and myeloid markers.

The panel of markers used will be appropriate to the clinical comments supplied , or as arranged by prior consultation.

Sample store at Room temperature. Samples are not stable for more than 24 hours. Hence they must be recieved by the Flow Cytometry lab at Box Hill within normal business hours Monday to Friday.

Cutoff for outpatirnt sample collection on Fridays is at 12:00 PM and MUST reach the laboratory by 4.00PM.

Flow cytometric analysis of cells stained by immunofluorescence with a range of antibodies against lymphoid and myeloid markers.

The panel of markers used will be appropriate to the clinical comments supplied , or as arranged by prior consultation.

Notes

 

 

  • Samples must be marked 'URGENT', must reach the laboratory as soon as possible. 
  • All out-of-hours testing requires notification.
  • Time and date of collection must be recorded on request form.

    Whole blood - 1 x EDTA sample, do not spin. Store at room temperature. An FBE must also be requested, needs a separate EDTA sample.

    Bone marrow aspirate - Sample into  EDTA tube

    TissueFresh (if to be shared with other departments for other tests eg. Histopathology or Microbiology) or RPMI fluid (especially if specimen is taken out of hours). This fluid is obtainable from: BH Lab 9895 3884, MH Lab: 9871 3572 or AH Lab: 9764 6136.

  • CSF/other fluids - at least 2mL is preferred

  • Collect CSF into three separate, sterile, screw-capped tubes provided (labelled 1,2 & 3)

  • Tube 1 is for Biochemistry (for Protein and Glucose) 0.5ml (10 drops) required 
  • Tube 2 for Cytology 1.0ml (20 drops)  and/or Flow Cytometry (6ml)
    • For Flow Cytometry a minimum of 6ml is required  in Tube 2
    • For Flow Cytometry and Cytology a minimum of 7ml is required in Tube 2     
  • Tube 3 for Microbiology upto 10ml (Full tube)

    If querying CJD the laboratory requires a 4th unopened tube containing at least 1ml of non-bloodstained CSF.

    Xanthochromia investigation requires a 4th tube containing 1ml of CSF .

 

 

Samples must be marked 'URGENT', must reach the laboratory as soon as possible. 

All out-of-hours testing requires notification.

Time and date of collection must be recorded on request form.

Whole blood - 1 x EDTA sample, do not spin. Store at room temperature. An FBE must also be requested, needs a separate EDTA sample.

Bone marrow aspirate - Sample into  EDTA tube

TissueFresh (if to be shared with other departments for other tests eg. Histopathology or Microbiology) or RPMI fluid (especially if specimen is taken out of hours). This fluid is obtainable from: BH Lab 9895 3884, MH Lab: 9871 3572 or AH Lab: 9764 6136.

CSF/other fluids - at least 2mL is preferred

Collect CSF into three separate, sterile, screw-capped tubes provided (labelled 1,2 & 3)

Tube 1 is for Biochemistry (for Protein and Glucose) 0.5ml (10 drops) required 

Tube 2 for Cytology 1.0ml (20 drops)  and/or Flow Cytometry (6ml)

For Flow Cytometry a minimum of 6ml is required  in Tube 2

For Flow Cytometry and Cytology a minimum of 7ml is required in Tube 2     

Tube 3 for Microbiology upto 10ml (Full tube)

If querying CJD the laboratory requires a 4th unopened tube containing at least 1ml of non-bloodstained CSF.

Xanthochromia investigation requires a 4th tube containing 1ml of CSF .

Leucocyte Immunophenotyping

Ordering information:

To order Leucocyte immunophenotyping in EMR type "FLOW CYTOMETRY" in the search window and select the relevant panel required based on clinical question.

Testing may be indicated when leukaemia (myeloid or lymphoid either T-cell or B-cell) to assess lineage or lymphoma/lymphoproliferative disorder is suspected.

Clinical data is valuable for Flow Cytometry staff to assist interpretation.

PLEASE NOTE : Flow Cytometry is a specialised procedure that is performed only during normal business hours.  If there is an extremely urgent need to perform this test outside of these hours, the laboratory must be contacted before the sample is collected. 

It is also imperative that the reason(s) for requesting flow cytometry are clearly indicated on the request form, including contact details for the requesting doctor.

Alternate names:
Cell Surface Markers, FACS Analysis, Flow Cytometry, Immunophenotyping
Laboratory:
Flow Cytometry
Test Code:
LYMP, LEUK
Specimen types:
Cerebrospinal Fluid
Fluid
Tissue
Bone marrow
Blood
Container types:
EDTA
Sterile Jar
EDTA
Collection Instructions:

Sample store at Room temperature. Samples are not stable for more than 24 hours. Hence they must be received by the Flow Cytometry lab at Box Hill within normal business hours Monday to Friday.

Cut-off for outpatient sample collection on Fridays is at 11.30 AM and MUST reach the laboratory before 4.00PM.

Flow cytometric analysis of cells stained by immunofluorescence with a range of antibodies against lymphoid and myeloid markers.

The panel of markers used will be appropriate to the panel selected and the clinical comments supplied , or as arranged by prior consultation

Notes:

Samples must be marked 'URGENT', must reach the laboratory as soon as possible. 

All out-of-hours testing requires notification.

Time and date of collection must be recorded on request form.

Whole blood - 1 x EDTA sample, do not spin. Store at room temperature. An FBE and BLOOD FILM must also be requested,  and needs a separate EDTA sample.

Bone marrow aspirate - Sample into  EDTA tube

TissueFresh (if to be shared with other departments for other tests eg. Histopathology or Microbiology) or RPMI fluid (especially if specimen is taken out of hours). This fluid is obtainable from: BH Lab 9895 3884, MH Lab: 9871 3572 or AH Lab: 9764 6136.

CSF/other fluids

Depending on the tests requested, 3-4 separate tubes will need to be provided. Note that the tubes are numbered so that order of collection can be identified – this order should be carefully followed, so that the optimal sample can be used for the requested tests. It is also important to consider the tests that are required, as this will determine how much CSF is needed in each tube. See the matrix below for details.

TUBE 1 is used for Biochemistry and Flow Cytometry. (Centrifuged in the lab – the supernatant is used for Biochemistry analysis (glucose, protein) and the cellular pellet will be used for flow cytometry if required). If tube 3 is bloodstained, take an aliquot for microbiology.

TUBE 2 is used for Cytology

TUBE 3 is for Microbiological analysis (cell count, Gram stain and culture) and any further infectious testing (e.g. viral PCR, mycobacterial PCR and culture, cryptococcal antigen).

TUBE 4 will be required only for;

(a) Creutzfeld-Jacob Disease (CJD) testing OR

(b) Xanthochromia analysis (wrap the tube in foil to prevent sample deterioration due to light).

 

TESTS REQUIRED

MINIMUM REQUIRED VOLUME (mL)

TUBE 1

TUBE 2

TUBE 3

TUBE 4

CHEMISTRY

1.0

 

 

 

ADD FLOW CYTOMETRY

Minimum 5.0-7.0

 

 

 

CYTOLOGY

 

1.0-3.0

 

 

MICROBIOLOGY

 

 

1.0-2.0

 

ADD OTHER INFECTIOUS MARKERS

 

 

add 2.0- 5.0-

 

ADD MYCOBACTERIAL CULTURE

 

 

Add 6.0-10.00

 

ADD CJD

 

 

 

1.0

ADD XANTHOCHROMIA

 

 

 

1.0 (foil)

 

Note, the ability to add further tests to a previously collected sample is dependent upon the volume provided. Also, many tests (flow cytometry, cytology) can only be performed on fresh specimens.

 

Other specialised testing (e.g. oligoclonal bands) will be performed using the supplied samples.

Xanthochromia and CJD analysis cannot be performed on a blood-stained sample

  

 

If EMR is not available, Pathology results are available from Auscare.  If you do not have an Auscare login,please complete attached form. https://pathology.easternhealth.org.au/handbook/downloads/Auscare%20access.pdf

Instruction on how to use Auscare - https://pathology.easternhealth.org.au/handbook/downloads/Auscare%20instructions.pdf

SPECIMEN COLLECTION MODULE (SCM) FAQs - CLICK HERE

CLICK HERE for a consent form to perform tests not covered by Medicare

TURN AROUND TIMES - When will my result be available?

CLICK HERE for Minimum Labelling Requirements

CLICK HERE for a printable Pathology request form

CLICK HERE for a Log sheet for recording Pathology results

"THE ART OF CCCCOLLECTION"

PLEASE CLICK HERE FOR THE PREFERRED ORDER OF DRAW