Date

Field

Changed From

Changed To

Assay name

Leucocyte Immunophenotyping

Plasma Cell Flow Cytometry Panel

Request Group

LYMP, LEUK

Flow

Volume (Adults)

1 mL

Preferred Container Type

EDTA
Sterile Jar

EDTA
Sterile Jar
CSF

Collection & Request Instructions

Sample store at Room temperature. Samples are not stable for more than 24 hours. Hence they must be received by the Flow Cytometry lab at Box Hill within normal business hours Monday to Friday.

Cut-off for outpatient sample collection on Fridays is at 11.30 AM and MUST reach the laboratory before 4.00PM.

Flow cytometric analysis of cells stained by immunofluorescence with a range of antibodies against lymphoid and myeloid markers.

The panel of markers used will be appropriate to the panel selected and the clinical comments supplied , or as arranged by prior consultation

Sample store at Room temperature. Samples are not stable for more than 24 hours. Hence they must be received by the Flow Cytometry lab at Box Hill within normal business hours Monday to Friday.

Cut-off for outpatient sample collection on Fridays is at 11.30 AM and MUST reach the laboratory before 2.00PM.

Flow cytometric analysis of cells stained by immunofluorescence with a range of antibodies against lymphoid and myeloid markers.

The panel of markers used will be appropriate to the panel selected and the clinical comments supplied , or as arranged by prior consultation

Frequency

Daily

Notes

Samples must be marked 'URGENT', must reach the laboratory as soon as possible. 

All out-of-hours testing requires notification.

Time and date of collection must be recorded on request form.

Whole blood - 1 x EDTA sample, do not spin. Store at room temperature. An FBE and BLOOD FILM must also be requested,  and needs a separate EDTA sample.

Bone marrow aspirate - Sample into  EDTA tube

Tissue - Fresh (if to be shared with other departments for other tests eg. Histopathology or Microbiology) or RPMI fluid (especially if specimen is taken out of hours). This fluid is obtainable from: BH Lab 9895 3884, MH Lab: 9871 3572 or AH Lab: 9764 6136.

CSF/other fluids

Depending on the tests requested, 3-4 separate tubes will need to be provided. Note that the tubes are numbered so that order of collection can be identified – this order should be carefully followed, so that the optimal sample can be used for the requested tests. It is also important to consider the tests that are required, as this will determine how much CSF is needed in each tube. See the matrix below for details.

TUBE 1 is used for Biochemistry and Flow Cytometry. (Centrifuged in the lab – the supernatant is used for Biochemistry analysis (glucose, protein) and the cellular pellet will be used for flow cytometry if required). If tube 3 is bloodstained, take an aliquot for microbiology.

TUBE 2 is used for Cytology

TUBE 3 is for Microbiological analysis (cell count, Gram stain and culture) and any further infectious testing (e.g. viral PCR, mycobacterial PCR and culture, cryptococcal antigen).

TUBE 4 will be required only for;

(a) Creutzfeld-Jacob Disease (CJD) testing OR

(b) Xanthochromia analysis (wrap the tube in foil to prevent sample deterioration due to light).

Note, the ability to add further tests to a previously collected sample is dependent upon the volume provided. Also, many tests (flow cytometry, cytology) can only be performed on fresh specimens.

Other specialised testing (e.g. oligoclonal bands) will be performed using the supplied samples.

Xanthochromia and CJD analysis cannot be performed on a blood-stained sample

Samples must be marked 'URGENT', must reach the laboratory as soon as possible. 

All out-of-hours testing requires notification.

Time and date of collection must be recorded on request form.

Whole blood - 1 x EDTA sample, do not spin. Store at room temperature. An FBE and BLOOD FILM must also be requested,  and needs a separate EDTA sample.

Bone marrow aspirate - Sample into  EDTA tube

Tissue - Fresh (if to be shared with other departments for other tests eg. Histopathology or Microbiology) or RPMI fluid (especially if specimen is taken out of hours). This fluid is obtainable from: BH Lab 9895 3884, MH Lab: 9871 3572 or AH Lab: 9764 6136.

CSF/other fluids

Depending on the tests requested, 3-4 separate tubes will need to be provided. Note that the tubes are numbered so that order of collection can be identified – this order should be carefully followed, so that the optimal sample can be used for the requested tests. It is also important to consider the tests that are required, as this will determine how much CSF is needed in each tube. See the matrix below for details.

TUBE 1 is used for Biochemistry and Flow Cytometry. (Centrifuged in the lab – the supernatant is used for Biochemistry analysis (glucose, protein) and the cellular pellet will be used for flow cytometry if required). If tube 3 is bloodstained, take an aliquot for microbiology.

TUBE 2 is used for Cytology

TUBE 3 is for Microbiological analysis (cell count, Gram stain and culture) and any further infectious testing (e.g. viral PCR, mycobacterial PCR and culture, cryptococcal antigen).

TUBE 4 will be required only for;

(a) Creutzfeld-Jacob Disease (CJD) testing OR

(b) Xanthochromia analysis (wrap the tube in foil to prevent sample deterioration due to light).

Note, the ability to add further tests to a previously collected sample is dependent upon the volume provided. Also, many tests (flow cytometry, cytology) can only be performed on fresh specimens.

Other specialised testing (e.g. oligoclonal bands) will be performed using the supplied samples.

Xanthochromia and CJD analysis cannot be performed on a blood-stained sample