A request slip indicating the clinical notes, prefered FISH probe if known and tissue type.
The follwing 2 micron sections of tissue on positively charged slides (as per immunohistochemistry), clearly labelled with patient name and section thickness must be sent:
a. 4 patient slides per probe. NOTE: For MYC testing a minimum of 6 slides are required. For >2 probes eg. "double hit" samples a minimum of 9 slides are required.
b. 1 H&E stained slide of the section intended for FISH with a marker indicating the optimal region for FISH - ie. the region with the most concentrated area of tumour.
See attached referal instruction sheet: http://pathology.easternhealth.org.au/handbook/downloads/anatomical-pathology/FISH_VCGS_instructions_2018.pdf