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PLEASE NOTE: Recent changes have been made to this Test


 Recent changes for Cerebrospinal Fluid - Cytology

Recent changes for Cerebrospinal Fluid - Cytology

Date
Field
Changed From
Changed To
27th April 2021
Collection & Request Instructions

The sample with the least amount of blood is prefered for Cytology.

CSF SPECIMEN INFORMATION – LABORATORY PROCESSING

Depending on the tests requested, 3-4 separate tubes will need to be provided.  Note that the tubes are numbered so that order of collection can be identified – this order should be carefully followed, so that the optimal sample can be used for the requested tests.  It is also important to consider the tests that are required, as this will determine how much CSF is needed in each tube.  See the matrix below for details.

TUBE 1 is used for Cytology.

TUBE 2 is used for Glucose, Protein and Flow Cytometry.

(centrifuged in the lab – the supernatant is used for Biochemistry analysis (glucose, protein) and the cellular pellet will be used for flow cytometry if required).

TUBE 3 is for Microbiological analysis (cell count, Gram stain and culture) and any further infectious testing (e.g. viral PCR, mycobacterial PCR and culture, cryptococcal antigen).

TUBE 4 will be required only for;

         (a) Creutzfeld-Jacob Disease (CJD) testing OR

(b) Xanthochromia analysis (wrap the tube in foil to prevent    sample deterioration due to light).

 

TESTS REQUIRED

MINIMUM REQUIRED VOLUME (mL)

TUBE 1

TUBE 2

TUBE 3

TUBE 4

CYTOLOGY. CHEMISTRY, MICRO/CULTURE

1.0-2.0

1.0

1.0-2.0

Nil

ADD FLOW CYTOMETRY

 

add 5.0-7.0

 

 

ADD OTHER INFECTIOUS MARKERS

 

 

add 2.0- 5.0-

 

ADD MYCOBACTERIAL CULTURE

 

 

Add 10.00

 

ADD CJD

 

 

 

1.0

ADD XANTHOCHROMIA

 

 

 

1.0 (foil)

 

Note, the ability to add further tests to a previously collected sample is dependent upon the volume provided.  Also, many tests (flow cytometry, cytology) can only be performed on fresh specimens.

 

Other specialised testing (e.g. oligoclonal bands) will be performed using the supplied samples.

 

Xanthochromia and CJD analysis cannot be performed on a blood-stained sample.

The sample with the least amount of blood is prefered for Cytology.

CSF SPECIMEN INFORMATION – LABORATORY PROCESSING

Depending on the tests requested, 3-4 separate tubes will need to be provided.  Note that the tubes are numbered so that order of collection can be identified – this order should be carefully followed, so that the optimal sample can be used for the requested tests.  It is also important to consider the tests that are required, as this will determine how much CSF is needed in each tube.  See the matrix below for details.

TUBE 1 is used for Biochemistry and Flow Cytometry. (Centrifuged in the lab – the supernatant is used for Biochemistry analysis (glucose, protein) and the cellular pellet will be used for flow cytometry if required). If tube 3 is bloodstained, take an aliquot for microbiology.

TUBE 2 is used for Cytology

TUBE 3 is for Microbiological analysis (cell count, Gram stain and culture) and any further infectious testing (e.g. viral PCR, mycobacterial PCR and culture, cryptococcal antigen).

TUBE 4 will be required only for;

(a) Creutzfeld-Jacob Disease (CJD) testing OR

(b) Xanthochromia analysis (wrap the tube in foil to prevent sample deterioration due to light).

 

TESTS REQUIRED

MINIMUM REQUIRED VOLUME (mL)

TUBE 1

TUBE 2

TUBE 3

TUBE 4

CHEMISTRY

1.0

     

ADD FLOW CYTOMETRY

Minimum 5.0-7.0

     

CYTOLOGY

 

1.0-3.0

   

MICROBIOLOGY

   

1.0-2.0

 

ADD OTHER INFECTIOUS MARKERS

   

add 2.0- 5.0-

 

ADD MYCOBACTERIAL CULTURE

   

Add 6.0-10.00

 

ADD CJD

     

1.0

ADD XANTHOCHROMIA

     

1.0 (foil)

 

Note, the ability to add further tests to a previously collected sample is dependent upon the volume provided. Also, many tests (flow cytometry, cytology) can only be performed on fresh specimens.

 

Other specialised testing (e.g. oligoclonal bands) will be performed using the supplied samples.

Xanthochromia and CJD analysis cannot be performed on a blood-stained sample.

 

 

Method

CSF sample processing:

If Cytology/Flow Cytometry requested  : Tube 1-Biochemistry, Tube 2- Cytology/Flow Cytometry, Tube 3-Microbiology

If Cytology/Flow Cytometry not requested: Tube 1 and 3-Microbiology, Tube 2-Biochemistry

Any confusion between the terms cell count and cytology could be cleared up when the cell count is rung through to the requesting doctor by the scientist. At this point it could be determined whether cytology is a genuine request or not (all to be documented in F8 notes).

 

 

14th August 2020
Collection & Request Instructions

The sample with the least amount of blood is prefered for Cytology.

Collect CSF into three separate, sterile, screw-capped tubes provided (labelled 1,2 & 3)

Tube 1 is for Biochemistry (for Protein and Glucose) 0.5ml (10 drops) required

Tube 2 for Cytology 1.0ml (20 drops)  and/or Flow Cytometry (6ml)

For Flow Cytometry a minimum of 6ml is required  in Tube 2

For Flow Cytometry and Cytology a minimum of 7ml is required in Tube 2    

Tube 3 for Microbiology upto 10ml (Full tube)

Xanthochromia investigation requires a 4th tube containing 1ml of CSF.

The sample with the least amount of blood is prefered for Cytology.

CSF SPECIMEN INFORMATION – LABORATORY PROCESSING

Depending on the tests requested, 3-4 separate tubes will need to be provided.  Note that the tubes are numbered so that order of collection can be identified – this order should be carefully followed, so that the optimal sample can be used for the requested tests.  It is also important to consider the tests that are required, as this will determine how much CSF is needed in each tube.  See the matrix below for details.

TUBE 1 is used for Cytology.

TUBE 2 is used for Glucose, Protein and Flow Cytometry.

(centrifuged in the lab – the supernatant is used for Biochemistry analysis (glucose, protein) and the cellular pellet will be used for flow cytometry if required).

TUBE 3 is for Microbiological analysis (cell count, Gram stain and culture) and any further infectious testing (e.g. viral PCR, mycobacterial PCR and culture, cryptococcal antigen).

TUBE 4 will be required only for;

         (a) Creutzfeld-Jacob Disease (CJD) testing OR

(b) Xanthochromia analysis (wrap the tube in foil to prevent    sample deterioration due to light).

 

TESTS REQUIRED

MINIMUM REQUIRED VOLUME (mL)

TUBE 1

TUBE 2

TUBE 3

TUBE 4

CYTOLOGY. CHEMISTRY, MICRO/CULTURE

1.0-2.0

1.0

1.0-2.0

Nil

ADD FLOW CYTOMETRY

 

add 5.0-7.0

 

 

ADD OTHER INFECTIOUS MARKERS

 

 

add 2.0- 5.0-

 

ADD MYCOBACTERIAL CULTURE

 

 

Add 10.00

 

ADD CJD

 

 

 

1.0

ADD XANTHOCHROMIA

 

 

 

1.0 (foil)

 

Note, the ability to add further tests to a previously collected sample is dependent upon the volume provided.  Also, many tests (flow cytometry, cytology) can only be performed on fresh specimens.

 

Other specialised testing (e.g. oligoclonal bands) will be performed using the supplied samples.

 

Xanthochromia and CJD analysis cannot be performed on a blood-stained sample.

Processing Instructions

Must be stored in the fridge.

 

Must be stored in the fridge.

Out of hours CSF samples for routine testing can be stored in the AP refrigerator.

 

Notes

Out of hours CSF samples for routine testing can be stored in the AP refrigerator.

 

 

 

Cerebrospinal Fluid - Cytology

Ordering information:

Cerner orderable: Cytology CSF

When ordering with other CSF tests please order in the same ordering conversation.

Out of businees hours urgent CSF cytology must be requested via discussion with the on call pathologist.

Alternate names:
CSF Cytology, Cytology CSF
Laboratory:
Cytopathology
Specimen types:
Cerebrospinal Fluid
Container types:
CSFCSF
Collection Instructions:

The sample with the least amount of blood is prefered for Cytology.

CSF SPECIMEN INFORMATION – LABORATORY PROCESSING

Depending on the tests requested, 3-4 separate tubes will need to be provided.  Note that the tubes are numbered so that order of collection can be identified – this order should be carefully followed, so that the optimal sample can be used for the requested tests.  It is also important to consider the tests that are required, as this will determine how much CSF is needed in each tube.  See the matrix below for details.

TUBE 1 is used for Biochemistry and Flow Cytometry. (Centrifuged in the lab – the supernatant is used for Biochemistry analysis (glucose, protein) and the cellular pellet will be used for flow cytometry if required). If tube 3 is bloodstained, take an aliquot for microbiology.

TUBE 2 is used for Cytology

TUBE 3 is for Microbiological analysis (cell count, Gram stain and culture) and any further infectious testing (e.g. viral PCR, mycobacterial PCR and culture, cryptococcal antigen).

TUBE 4 will be required only for;

(a) Creutzfeld-Jacob Disease (CJD) testing OR

(b) Xanthochromia analysis (wrap the tube in foil to prevent sample deterioration due to light).

 

TESTS REQUIRED

MINIMUM REQUIRED VOLUME (mL)

TUBE 1

TUBE 2

TUBE 3

TUBE 4

CHEMISTRY

1.0

     

ADD FLOW CYTOMETRY

Minimum 5.0-7.0

     

CYTOLOGY

 

1.0-3.0

   

MICROBIOLOGY

   

1.0-2.0

 

ADD OTHER INFECTIOUS MARKERS

   

add 2.0- 5.0-

 

ADD MYCOBACTERIAL CULTURE

   

Add 6.0-10.00

 

ADD CJD

     

1.0

ADD XANTHOCHROMIA

     

1.0 (foil)

 

Note, the ability to add further tests to a previously collected sample is dependent upon the volume provided. Also, many tests (flow cytometry, cytology) can only be performed on fresh specimens.

 

Other specialised testing (e.g. oligoclonal bands) will be performed using the supplied samples.

Xanthochromia and CJD analysis cannot be performed on a blood-stained sample.

 

 

Notes:

 

 

Result Turnaround Time:
72 hours
Laboratory Use Only

  

 

If EMR is not available, Pathology results are available from Auscare.  If you do not have an Auscare login,please complete attached form. https://pathology.easternhealth.org.au/handbook/downloads/Auscare%20access.pdf

Instruction on how to use Auscare - https://pathology.easternhealth.org.au/handbook/downloads/Auscare%20instructions.pdf

SPECIMEN COLLECTION MODULE (SCM) FAQs - CLICK HERE

CLICK HERE for a consent form to perform tests not covered by Medicare

TURN AROUND TIMES - When will my result be available?

CLICK HERE for Minimum Labelling Requirements

CLICK HERE for a printable Pathology request form

CLICK HERE for a Log sheet for recording Pathology results

"THE ART OF CCCCOLLECTION"

PLEASE CLICK HERE FOR THE PREFERRED ORDER OF DRAW