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PLEASE NOTE: Recent changes have been made to this Test


 Recent changes for Lymphocyte subsets

Recent changes for Lymphocyte subsets

Date
Field
Changed From
Changed To
14th October 2021
Alternative names

CD3 count, CD4 count, CD8 count, CD4:CD8 ratio CD19 count, CD56 count, flow cytometry

14th October 2021
Alternative names

CD19 count & CD56 count, CD4 count, CD4 count, CD4:CD8 ratio Lymphocyte subsets include: CD3 count, CD4:CD8 ratio, CD8 count, CD8 count, T subsets include: CD3 count

T subsets include: CD3 count, CD4 count, CD8 count, CD4:CD8 ratio Lymphocyte subsets include: CD3 count, CD4 count, CD8 count, CD4:CD8 ratio, CD19 count & CD56 count Flow Cytometry

Collection & Request Instructions

Sample store at Room temperature. Samples are not stable for more than 24 hours. Hence they must be received by the Flow Cytometry lab at Box Hill within normal business hours Monday to Friday.

Cut-off for outpatient sample collection on Fridays is at 11.30 AM and MUST reach the laboratory before 4.00PM.

Flow cytometric analysis of cells stained by immunofluorescence with a range of antibodies against lymphoid and myeloid markers.

The panel of markers used will be appropriate to the panel selected and the clinical comments supplied, or as arranged by prior consultation.

Sample store at ROOM TEMPERATURE. Samples are not stable for more than 24 hours. Hence they must be received by the Flow Cytometry lab at Box Hill within normal business hours Monday to Friday.

Cut-off for outpatient sample collection on Fridays is at 11.30 AM and MUST reach the laboratory before 4.00PM.

Flow cytometric analysis of cells stained by immunofluorescence with a range of antibodies against lymphoid and myeloid markers.

The panel of markers used will be appropriate to the panel selected and the clinical comments supplied, or as arranged by prior consultation.

14th October 2021
Collection & Request Instructions

The samples are not stable for more than 24 hours. Hence all samples must be recieved in the laboratory at Box Hill  Monday to Friday within business hours.

Cutoff for outpatient sample collection on Fridays is at 11.30 AM and MUST reach the laboratory before 4.00PM.

 

Sample store at Room temperature. Samples are not stable for more than 24 hours. Hence they must be received by the Flow Cytometry lab at Box Hill within normal business hours Monday to Friday.

Cut-off for outpatient sample collection on Fridays is at 11.30 AM and MUST reach the laboratory before 4.00PM.

Flow cytometric analysis of cells stained by immunofluorescence with a range of antibodies against lymphoid and myeloid markers.

The panel of markers used will be appropriate to the panel selected and the clinical comments supplied, or as arranged by prior consultation.

Frequency

Daily

Notes

Used to monitor T-cell numbers

Preferred EDTA sample. Do not spin, store at room temperature.

Samples must be marked 'URGENT', must reach the laboratory as soon as possible. 

All out-of-hours testing requires notification.

Time and date of collection must be recorded on request form.

Whole blood - 1 x EDTA sample, do not spin. Store at room temperature. An FBE must also be requested, and needs a separate EDTA sample.

14th October 2021
Assay name

T Cell Subsets

Lymphocyte subsets

Alternative names

CD2, CD3, CD4, CD8

T subsets include: CD3 count, CD4 count, CD8 count, CD4:CD8 ratio Lymphocyte subsets include: CD3 count, CD4 count, CD8 count, CD4:CD8 ratio, CD19 count & CD56 count

Request Group

FLOW

TSUB & LSUB

20th July 2021
Processing Instructions

Prefer EDTA sample.

Do not spin, store at room temperature.

Prefer EDTA sample.

Do not spin, store at room temperature.

20th July 2021
Notes

Used to monitor T-cell numbers

Preferred EDTA sample. Do not spin, store at room temperature.

Used to monitor T-cell numbers

Preferred EDTA sample. Do not spin, store at room temperature.

20th July 2021
Collection & Request Instructions

The samples are not stable for more than 24 hours. Hence all samples must be recieved in the laboratory at Box Hill  Monday to Friday within business hours.

The samples are not stable for more than 24 hours. Hence all samples must be recieved in the laboratory at Box Hill  Monday to Friday within business hours.

Cutoff for outpatient sample collection on Fridays is at 11.30 AM and MUST reach the laboratory before 4.00PM.

 

Processing Instructions

Prefer EDTA sample.

Do not spin, store at room temperature.

Prefer EDTA sample.

Do not spin, store at room temperature.

Notes

Used to monitor T-cell numbers

Preferred EDTA sample. Do not spin, store at room temperature.

Used to monitor T-cell numbers

Preferred EDTA sample. Do not spin, store at room temperature.

Lymphocyte subsets

Ordering information:

To order lymphocyte or T cell subsets in EMR type "FLOW CYTOMETRY" in the search window and select the relevant panel required based on the test required.

Testing may be indicated in immunodeficiency or as part of monitoring of drug therapy.

Clinical data is valuable for Flow Cytometry staff to assist interpretation.

T subsets include: CD3 count, CD4 count, CD8 count, CD4:CD8 ratio

Lymphocyte subsets include: CD3 count, CD4 count, CD8 count, CD4:CD8 ratio, CD19 count & CD56 count

PLEASE NOTE: Flow Cytometry is a specialised procedure that is performed only during normal business hours.  If there is an extremely urgent need to perform this test outside of these hours, the laboratory must be contacted before the sample is collected. 

It is also imperative that the reason(s) for requesting flow cytometry are clearly indicated on the request form, including contact details for the requesting doctor.

Alternate names:
CD19 count, CD3 count, CD4 count, CD4:CD8 ratio, CD56 count, CD8 count, flow cytometry
Laboratory:
Flow Cytometry
Test Code:
TSUB & LSUB
Specimen types:
Blood
Container types:
EDTA
Lithium heparin no gel
EDTA
Collection Instructions:

Sample store at ROOM TEMPERATURE. Samples are not stable for more than 24 hours. Hence they must be received by the Flow Cytometry lab at Box Hill within normal business hours Monday to Friday.

Cut-off for outpatient sample collection on Fridays is at 11.30 AM and MUST reach the laboratory before 4.00PM.

Flow cytometric analysis of cells stained by immunofluorescence with a range of antibodies against lymphoid and myeloid markers.

The panel of markers used will be appropriate to the panel selected and the clinical comments supplied, or as arranged by prior consultation.

Notes:

Samples must be marked 'URGENT', must reach the laboratory as soon as possible. 

All out-of-hours testing requires notification.

Time and date of collection must be recorded on request form.

Whole blood - 1 x EDTA sample, do not spin. Store at room temperature. An FBE must also be requested, and needs a separate EDTA sample.

Frequency:
Daily
Result Turnaround Time:
1 days
Reference Interval:
   
   
   
   
   
Laboratory Use Only

  

 

If EMR is not available, Pathology results are available from Auscare.  If you do not have an Auscare login,please complete attached form. https://pathology.easternhealth.org.au/handbook/downloads/Auscare%20access.pdf

Instruction on how to use Auscare - https://pathology.easternhealth.org.au/handbook/downloads/Auscare%20instructions.pdf

SPECIMEN COLLECTION MODULE (SCM) FAQs - CLICK HERE

CLICK HERE for a consent form to perform tests not covered by Medicare

TURN AROUND TIMES - When will my result be available?

CLICK HERE for Minimum Labelling Requirements

CLICK HERE for a printable Pathology request form

CLICK HERE for a Log sheet for recording Pathology results

"THE ART OF CCCCOLLECTION"

PLEASE CLICK HERE FOR THE PREFERRED ORDER OF DRAW