Collect CSF into three separate, sterile, screw-capped tubes provided (labelled 1,2 & 3)
Tube 1 is for Biochemistry (for Protein and Glucose) 0.5ml (10 drops) required
Tube 2 for Cytology 1.0ml (20 drops) and/or Flow Cytometry (6ml)
For Flow Cytometry a minimum of 6ml is required in Tube 2
For Flow Cytometry and Cytology a minimum of 7ml is required in Tube 2
Tube 3 for Microbiology upto 10ml (Full tube)
If querying CJD the laboratory requires a 4th unopened tube containing at least 1ml of non-bloodstained CSF.
Xanthochromia investigation requires a 4th tube containing 1ml of CSF .
Cell Reference Ranges
0 - 5 lymphocytes (x10*6/L)
(£ 1 month)
0 – 20 white cells (x10*6/L, predominantly lymphocytes)
0 - several hundred erythrocytes
Leucocyte:erythrocyte (WBC:RBC) ratio is normally 1:500 (including traumatic tap).