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PLEASE NOTE: Recent changes have been made to this Test


 Recent changes for Lupus Anticoagulant Screen

Recent changes for Lupus Anticoagulant Screen

Date
Field
Changed From
Changed To
3rd May 2021
External Transport Instructions

Samples can be transported frozen to the laboratory if the facilities are available to double spin and aliquot these sample. This has to be done within 4 hours.

Unspun sample is stable for up to 4 hours and must be transport to laboratory immediately. (Sample must be double spun, aliquoted and frozen within 4 hours from time of collection).

Samples can be transported frozen to the laboratory if the facilities are available to double spin and aliquot these samples. This has to be performed within 4 hours of collection.

Unspun sample is stable for up to 4 hours and must be transport to laboratory immediately. (Sample must be double spun, aliquoted and frozen within 4 hours from time of collection).

3rd May 2021
Collection & Request Instructions

Notification of anticoagulant therapy is essential

One (1x) Sodium citrate tubes is required. Do not use 1 ml citrate tubes.

Filled to label marker and mixed by inversion

The patient must not be receiving heparin

Transport to laboratory immediately. (Sample must be double spun and frozen within 4 hours from time of collection).

Tests performed:

INR, APTT, APTT Mix, Silica Clotting Time and Dilute Russell's Viper venom time (LA screen/Confirm).

 

Notification of anticoagulant therapy is essential

One (1x) Sodium citrate tubes is required. Do not use 1 ml citrate tubes.

Filled to label marker and mixed by inversion

The patient must not be receiving heparin

Unspun sample is stable for up to 4 hours and must be transport to laboratory immediately. (Sample must be double spun, aliquoted and frozen within 4 hours from time of collection).

 

Tests performed:

INR, APTT, APTT Mix, Silica Clotting Time and Dilute Russell's Viper venom time (LA screen/Confirm).

 

Processing Instructions

Transport sample to laboratory within 4 hours of collection.

The sample MUST be double spun and frozen within the 4 hour time limit.

Unspun sample is stable for up to 4 hours and must be transport to laboratory immediately. (Sample must be double spun, aliquoted and frozen within 4 hours from time of collection).

External Transport Instructions

Samples can be transported frozen to the laboratory if the facilities are available to double spin and aliquot these sample. This has to be done within 4 hours.

Unspun sample is stable for up to 4 hours and must be transport to laboratory immediately. (Sample must be double spun, aliquoted and frozen within 4 hours from time of collection).

3rd May 2021
Collection & Request Instructions

Notification of anticoagulant therapy is essential

One (1x) Sodium citrate tubes is required. Do not use 1 ml citrate tubes.

Filled to label marker and mixed by inversion

The patient must not be receiving heparin

Transport to laboratory immediately. (maximum of 4 hours from time of collection).

Tests performed:

INR, APTT, APTT Mix, Silica Clotting Time and Dilute Russell's Viper venom time (LA screen/Confirm).

 

Notification of anticoagulant therapy is essential

One (1x) Sodium citrate tubes is required. Do not use 1 ml citrate tubes.

Filled to label marker and mixed by inversion

The patient must not be receiving heparin

Transport to laboratory immediately. (Sample must be double spun and frozen within 4 hours from time of collection).

Tests performed:

INR, APTT, APTT Mix, Silica Clotting Time and Dilute Russell's Viper venom time (LA screen/Confirm).

 

Processing Instructions

Transport sample to laboratory within 4 hours of collection.

The sample MUST be double spun and frozen within the 4 hour time limit.

19th January 2021
Method

Centrifuge the two 3.5 mL citrate tubes and transfer the plasma to a labeled 4 mL plastic tube and re-centrifuge at room temperature (do not use the refrigerated centrifuge).

The double centrifuged plasma is transferred to two 1 mL eppendorf tubes and freeze.

Sample must be centrifuged within 4 hours of collection.

**Sample must be centrifuged within 4 hours of collection**

Centrifuge the two 3.5 mL citrate tubes (and transfer the plasma to a labeled 4 mL plastic tube and re-centrifuge at room temperature (do not use the refrigerated centrifuge).  One sample is acceptable but 2 samples are preferable for Lupus testing.

The double centrifuged plasma is transferred into a new labelled tubes. 

Double spun samples are then frozen in the -20'C freezer.

19th January 2021
Collection & Request Instructions

Notification of anticoagulant therapy is essential

One (1x) Sodium citrate tubes is required. Do not use 1 ml citrate tubes.

Filled to label marker and mixed by inversion

The patient must not be receiving heparin

Transport to laboratory immediately.

Tests performed:

INR, APTT, APTT Mix, Lupus sensitive APTT and Dilute Russell's Viper venom time (LA screen/Confirm).

 

Notification of anticoagulant therapy is essential

One (1x) Sodium citrate tubes is required. Do not use 1 ml citrate tubes.

Filled to label marker and mixed by inversion

The patient must not be receiving heparin

Transport to laboratory immediately. (maximum of 4 hours from time of collection).

Tests performed:

INR, APTT, APTT Mix, Silica Clotting Time and Dilute Russell's Viper venom time (LA screen/Confirm).

 

Method

Centrifuge the two 3.5 mL citrate tubes and transfer the plasma to a labeled 4 mL plastic tube and re-centrifuge at room temperature (do not use the refrigerated centrifuge).

The double centrifuged plasma is transferred to two 1 mL eppendorf tubes and freeze.

Centrifuge the two 3.5 mL citrate tubes and transfer the plasma to a labeled 4 mL plastic tube and re-centrifuge at room temperature (do not use the refrigerated centrifuge).

The double centrifuged plasma is transferred to two 1 mL eppendorf tubes and freeze.

Sample must be centrifuged within 4 hours of collection.

10th December 2020
Collection & Request Instructions

Notification of anticoagulant therapy is essential

Two (2x) Sodium citrate tubes required. Do not use 1 ml citrate tubes.

Filled to label marker and mixed by inversion

The patient must not be receiving heparin

Transport to laboratory immediately.

Tests performed:

INR, APTT, APTT Mix, Lupus sensitive APTT and Dilute Russell's Viper venom time (LA screen/Confirm).

 

Notification of anticoagulant therapy is essential

One (1x) Sodium citrate tubes is required. Do not use 1 ml citrate tubes.

Filled to label marker and mixed by inversion

The patient must not be receiving heparin

Transport to laboratory immediately.

Tests performed:

INR, APTT, APTT Mix, Lupus sensitive APTT and Dilute Russell's Viper venom time (LA screen/Confirm).

 

Lupus Anticoagulant Screen

Alternate names:
LA, LAC
Laboratory:
Coagulation
Test Code:
LAS
Specimen types:
Plasma
Container types:
Sodium CitrateSodium Citrate
Collection Instructions:

Notification of anticoagulant therapy is essential

One (1x) Sodium citrate tubes is required. Do not use 1 ml citrate tubes.

Filled to label marker and mixed by inversion

The patient must not be receiving heparin

Unspun sample is stable for up to 4 hours and must be transport to laboratory immediately. (Sample must be double spun, aliquoted and frozen within 4 hours from time of collection).

 

Tests performed:

INR, APTT, APTT Mix, Silica Clotting Time and Dilute Russell's Viper venom time (LA screen/Confirm).

 

Frequency:
Weekly
Result Turnaround Time:
2 weeks
Reference Interval:

LA screen   <1.2 Lupus anticoagulant not detected.

LA Confirm <1.2 Lupus anticoagulant not detected

                    1.2 - 1.5 L.A. weakly present

                    1.5 - 2.0 L.A. moderately present.

                     >2.0      L.A. strongly present

                                                

Laboratory Use Only

  

 

If EMR is not available, Pathology results are available from Auscare.  If you do not have an Auscare login,please complete attached form. https://pathology.easternhealth.org.au/handbook/downloads/Auscare%20access.pdf

Instruction on how to use Auscare - https://pathology.easternhealth.org.au/handbook/downloads/Auscare%20instructions.pdf

SPECIMEN COLLECTION MODULE (SCM) FAQs - CLICK HERE

CLICK HERE for a consent form to perform tests not covered by Medicare

TURN AROUND TIMES - When will my result be available?

CLICK HERE for Minimum Labelling Requirements

CLICK HERE for a printable Pathology request form

CLICK HERE for a Log sheet for recording Pathology results

"THE ART OF CCCCOLLECTION"

PLEASE CLICK HERE FOR THE PREFERRED ORDER OF DRAW