Recent changes for Cold Agglutinins |
Date | Field | Changed From | Changed To |
| 11th July 2024 | Collection & Request Instructions | Pre heated 6 mL EDTA tube. Tubes,needles and syringes must be pre-warmed at 37oC and specimen placed into a hot box (obtained from the laboratory) and transported to the laboratory immediately. Cold specimens may give erroneous results. | Collect a Hot Box with 2x pre-heated, 6 mL EDTA tube from Pathology Specimen Reception. Sample collection must occur within 10min. Once samples are collected, transport to the laboratory immediately.
Cold specimens may give erroneous results. |
| Processing Instructions | Specimen must be allowed to settle in 37°C incubator (tubes must NOT be allowed to cool to RT prior to separation). Once seperated, can be stored according to laboratory protocols. | Specimens must be handed to Blood Bank Scientist immediately to place in their waterbath. Please do not leave in the rack. |
| External Transport Instructions | Once plasma is separated, transportation and storage can be at 4°C. | Angliss and Maroondah lab: Once plasma is separated, transportation and storage can be at 4°C. |
| Frequency | Daily | Mon-Fri |
| Notes | Investigation of cold agglutinin specificity, thermal range and titre by consultation with Haematologist
Tubes warmed to 37oC are available from Blood Bank laboratory, Box Hill.
Test performed during business hours. | Test performed during business hours, Monday to Friday. |
| 18th June 2024 | Minimum/Paediatric Volume | | N/A mL |
| 18th June 2024 | Request Group | CAGS | CAGG |
| 18th June 2024 | Laboratory | Referred Test | Blood Bank |
| Volume (Adults) | | 5 mL |
| Preferred Container Type | Serum No Gel & EDTA | Pink EDTA (6mL) |
| Collection & Request Instructions | Pre heated 4 mL EDTA tube and 10 mL plain tube. Tubes,needles and syringes must be pre-warmed at 37oC and specimen placed into a thermos flask ( obtained from the laboratory) and transported to the laboratory immediately. Cold specimens may give erroneous results. | Pre heated 6 mL EDTA tube. Tubes,needles and syringes must be pre-warmed at 37oC and specimen placed into a hot box (obtained from the laboratory) and transported to the laboratory immediately. Cold specimens may give erroneous results. |
| Processing Instructions | Specimen must be centrifuged and separated immediately upon receipt (tubes must NOT be allowed to cool to RT prior to separation) | Specimen must be allowed to settle in 37°C incubator (tubes must NOT be allowed to cool to RT prior to separation). Once seperated, can be stored according to laboratory protocols. |
| External Laboratory | Austin Pathology | |
| External Transport Instructions | Once plasma is separated, transportation and storage can be at 4 degrees. | Once plasma is separated, transportation and storage can be at 4°C. |
| Frequency | As required | Daily |
| Notes | Investigation of cold agglutinin specificity, thermal range and titre by consultation with Haematologist
Tubes warmed to 37oC are available from the Core laboratory | Investigation of cold agglutinin specificity, thermal range and titre by consultation with Haematologist
Tubes warmed to 37oC are available from Blood Bank laboratory, Box Hill.
Test performed during business hours. |
