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PLEASE NOTE: Recent changes have been made to this Test


 Recent changes for Cerebrospinal Fluid - microscopy and culture

Recent changes for Cerebrospinal Fluid - microscopy and culture

Date
Field
Changed From
Changed To
27th April 2021
Notes

CSF SPECIMEN INFORMATION – LABORATORY PROCESSING

Depending on the tests requested, 3-4 separate tubes will need to be provided.  Note that the tubes are numbered so that order of collection can be identified – this order should be carefully followed, so that the optimal sample can be used for the requested tests.  It is also important to consider the tests that are required, as this will determine how much CSF is needed in each tube.  See the matrix below for details.

TUBE 1 is used for Cytology.

TUBE 2 is used for Glucose, Protein and Flow Cytometry.

(centrifuged in the lab – the supernatant is used for Biochemistry analysis (glucose, protein) and the cellular pellet will be used for flow cytometry if required).

TUBE 3 is for Microbiological analysis (cell count, Gram stain and culture) and any further infectious testing (e.g. viral PCR, mycobacterial PCR and culture, cryptococcal antigen).

TUBE 4 will be required only for;

         (a) Creutzfeld-Jacob Disease (CJD) testing OR

(b) Xanthochromia analysis (wrap the tube in foil to prevent    sample deterioration due to light).

 

TESTS REQUIRED

MINIMUM REQUIRED VOLUME (mL)

TUBE 1

TUBE 2

TUBE 3

TUBE 4

CYTOLOGY. CHEMISTRY, MICRO/CULTURE

1.0-2.0

1.0

1.0-2.0

Nil

ADD FLOW CYTOMETRY

 

add 5.0-7.0

 

 

ADD OTHER INFECTIOUS MARKERS

 

 

add 2.0- 5.0-

 

ADD MYCOBACTERIAL CULTURE

 

 

Add 10.00

 

ADD CJD

 

 

 

1.0

ADD XANTHOCHROMIA

 

 

 

1.0 (foil)

 

Note, the ability to add further tests to a previously collected sample is dependent upon the volume provided.  Also, many tests (flow cytometry, cytology) can only be performed on fresh specimens.

 

Other specialised testing (e.g. oligoclonal bands) will be performed using the supplied samples.

 

Xanthochromia and CJD analysis cannot be performed on a blood-stained sample.

Special tests available on request:

PCR for viruses

Acid-fast stain and/or culture for Mycobacteria

Cryptococcal antigen

Serological test for Syphilis (must be paired with 5mL serum sample or test will not be performed)

Viral Serology, if CSF is accompanied by serum sample

Flow Cytometry - requires a 4th tube

The turnaround time for CSF microscopy are 30-40 minutes.

The turnaround time for CSF culture are 3-5 days.

Depending on the tests requested, 3-4 separate tubes will need to be provided. Note that the tubes are numbered so that order of collection can be identified – this order should be carefully followed, so that the optimal sample can be used for the requested tests. It is also important to consider the tests that are required, as this will determine how much CSF is needed in each tube. See the matrix below for details.

TUBE 1 is used for Biochemistry and Flow Cytometry. (Centrifuged in the lab – the supernatant is used for Biochemistry analysis (glucose, protein) and the cellular pellet will be used for flow cytometry if required). If tube 3 is bloodstained, take an aliquot for microbiology.

TUBE 2 is used for Cytology

TUBE 3 is for Microbiological analysis (cell count, Gram stain and culture) and any further infectious testing (e.g. viral PCR, mycobacterial PCR and culture, cryptococcal antigen).

TUBE 4 will be required only for;

(a) Creutzfeld-Jacob Disease (CJD) testing OR

(b) Xanthochromia analysis (wrap the tube in foil to prevent sample deterioration due to light).

 

TESTS REQUIRED

MINIMUM REQUIRED VOLUME (mL)

TUBE 1

TUBE 2

TUBE 3

TUBE 4

CHEMISTRY

1.0

     

ADD FLOW CYTOMETRY

Minimum 5.0-7.0

     

CYTOLOGY

 

1.0-3.0

   

MICROBIOLOGY

   

1.0-2.0

 

ADD OTHER INFECTIOUS MARKERS

   

add 2.0- 5.0-

 

ADD MYCOBACTERIAL CULTURE

   

Add 6.0-10.00

 

ADD CJD

     

1.0

ADD XANTHOCHROMIA

     

1.0 (foil)

 

Note, the ability to add further tests to a previously collected sample is dependent upon the volume provided. Also, many tests (flow cytometry, cytology) can only be performed on fresh specimens.

 

Other specialised testing (e.g. oligoclonal bands) will be performed using the supplied samples.

Xanthochromia and CJD analysis cannot be performed on a blood-stained sample.

14th August 2020
Notes

Special tests available on request:

PCR for viruses

Acid-fast stain and/or culture for Mycobacteria

Cryptococcal antigen

Serological test for Syphilis (must be paired with 5mL serum sample or test will not be performed)

Viral Serology, if CSF is accompanied by serum sample

Flow Cytometry - requires a 4th tube

The turnaround time for CSF microscopy are 30-40 minutes.

The turnaround time for CSF culture are 3-5 days.

CSF SPECIMEN INFORMATION – LABORATORY PROCESSING

Depending on the tests requested, 3-4 separate tubes will need to be provided.  Note that the tubes are numbered so that order of collection can be identified – this order should be carefully followed, so that the optimal sample can be used for the requested tests.  It is also important to consider the tests that are required, as this will determine how much CSF is needed in each tube.  See the matrix below for details.

TUBE 1 is used for Cytology.

TUBE 2 is used for Glucose, Protein and Flow Cytometry.

(centrifuged in the lab – the supernatant is used for Biochemistry analysis (glucose, protein) and the cellular pellet will be used for flow cytometry if required).

TUBE 3 is for Microbiological analysis (cell count, Gram stain and culture) and any further infectious testing (e.g. viral PCR, mycobacterial PCR and culture, cryptococcal antigen).

TUBE 4 will be required only for;

         (a) Creutzfeld-Jacob Disease (CJD) testing OR

(b) Xanthochromia analysis (wrap the tube in foil to prevent    sample deterioration due to light).

 

TESTS REQUIRED

MINIMUM REQUIRED VOLUME (mL)

TUBE 1

TUBE 2

TUBE 3

TUBE 4

CYTOLOGY. CHEMISTRY, MICRO/CULTURE

1.0-2.0

1.0

1.0-2.0

Nil

ADD FLOW CYTOMETRY

 

add 5.0-7.0

 

 

ADD OTHER INFECTIOUS MARKERS

 

 

add 2.0- 5.0-

 

ADD MYCOBACTERIAL CULTURE

 

 

Add 10.00

 

ADD CJD

 

 

 

1.0

ADD XANTHOCHROMIA

 

 

 

1.0 (foil)

 

Note, the ability to add further tests to a previously collected sample is dependent upon the volume provided.  Also, many tests (flow cytometry, cytology) can only be performed on fresh specimens.

 

Other specialised testing (e.g. oligoclonal bands) will be performed using the supplied samples.

 

Xanthochromia and CJD analysis cannot be performed on a blood-stained sample.

Special tests available on request:

PCR for viruses

Acid-fast stain and/or culture for Mycobacteria

Cryptococcal antigen

Serological test for Syphilis (must be paired with 5mL serum sample or test will not be performed)

Viral Serology, if CSF is accompanied by serum sample

Flow Cytometry - requires a 4th tube

The turnaround time for CSF microscopy are 30-40 minutes.

The turnaround time for CSF culture are 3-5 days.

Cerebrospinal Fluid - microscopy and culture

Ordering information:

All other CSF tests, e.g.virus studies, Cryptococcal Antigen, Mycobacteria study, need to be ordered seperately in EMR.

*Hand deliver sample directly to laboratory*

Alternate names:
CSF - microscopy & culture, CSF culture, CSF M+C
Laboratory:
Microbiology
Test Code:
CSF
Specimen types:
Cerebrospinal Fluid
Container types:
CSFCSF
Collection Instructions:

Lumbar Puncture/CSF Collection Instructions

Notes:

Depending on the tests requested, 3-4 separate tubes will need to be provided. Note that the tubes are numbered so that order of collection can be identified – this order should be carefully followed, so that the optimal sample can be used for the requested tests. It is also important to consider the tests that are required, as this will determine how much CSF is needed in each tube. See the matrix below for details.

NB: 0.5mL is approximately 10 drops.

Up to 40 ml of CSF can be collected safely at the time of LP.

TUBE 1 is used for Biochemistry and Flow Cytometry. (Centrifuged in the lab – the supernatant is used for Biochemistry analysis (glucose, protein) and the cellular pellet will be used for flow cytometry if required). If tube 3 is bloodstained, take an aliquot for microbiology.

TUBE 2 is used for Cytology

TUBE 3 is for Microbiological analysis (cell count, Gram stain and culture) and any further infectious testing (e.g. viral PCR, mycobacterial PCR and culture, cryptococcal antigen).

TUBE 4 will be required only for;

(a) Creutzfeld-Jacob Disease (CJD) testing OR

(b) Xanthochromia analysis (wrap the tube in foil to prevent sample deterioration due to light).

 

TESTS REQUIRED

MINIMUM REQUIRED VOLUME (mL)

TUBE 1

TUBE 2

TUBE 3

TUBE 4

CHEMISTRY

1.0

     

ADD FLOW CYTOMETRY

Minimum 5.0-7.0

     

CYTOLOGY

 

1.0-3.0

   

MICROBIOLOGY

   

1.0-2.0

 

ADD OTHER INFECTIOUS MARKERS

   

add 2.0- 5.0-

 

ADD MYCOBACTERIAL CULTURE

   

Add 6.0-10.00

 

ADD CJD

     

1.0

ADD XANTHOCHROMIA

     

1.0 (foil)

 

Note, the ability to add further tests to a previously collected sample is dependent upon the volume provided. Also, many tests (flow cytometry, cytology) can only be performed on fresh specimens.

 

Other specialised testing (e.g. oligoclonal bands) will be performed using the supplied samples.

Xanthochromia and CJD analysis cannot be performed on a blood-stained sample.

Frequency:
Daily
Result Turnaround Time:
60 minutes
Reference Interval:
RBC   0 x 106/L
WBC

Lymphocytes

 

< 6 x 106/L
  Other WBC's 0 x 106/L
Neonates WBC < 25 x 106/L

Note: The normal ratio of white cells to erythrocytes is approximately 1:500

Laboratory Use Only

  

 

If EMR is not available, Pathology results are available from Auscare.  If you do not have an Auscare login,please complete attached form. https://pathology.easternhealth.org.au/handbook/downloads/Auscare%20access.pdf

Instruction on how to use Auscare - https://pathology.easternhealth.org.au/handbook/downloads/Auscare%20instructions.pdf

SPECIMEN COLLECTION MODULE (SCM) FAQs - CLICK HERE

CLICK HERE for a consent form to perform tests not covered by Medicare

TURN AROUND TIMES - When will my result be available?

CLICK HERE for Minimum Labelling Requirements

CLICK HERE for a printable Pathology request form

CLICK HERE for a Log sheet for recording Pathology results

"THE ART OF CCCCOLLECTION"

PLEASE CLICK HERE FOR THE PREFERRED ORDER OF DRAW