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Test & Collection Guide

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Vitamin D Metabolites

Ordering information:

Vitamin D testing can only be requested for a prescribed list of clinical situations (see list below).  The request form MUST include the clinical indication for the test.  In the absence of this information, testing cannot be performed.

PRESCRIBED LIST OF INDICATIONS FOR VITAMIN D TESTING

 

Signs or symptoms of osteoporosis or osteomalacia

 Increased alkaline phosphatase and otherwise normal LFT's

Hyperparathyroidism, hypo- or hypercalcaemia, or hypophosphataemia;

Malabsorption conditions

Deeply pigmented skin, or chronic and severe lack of sun exposure for cultural, medical, occupational or  residential reasons

Taking medication known to decrease 25OH-D levels (for example, anticonvulsants)

Chronic renal failure or renal transplant recipient

<16 years of age with signs or symptoms of rickets

An infant whose mother has established vitamin D deficiency

      Exclusively breastfed baby with at least one other risk factor mentioned in this list

Has a sibling who is less than 16 years of age and has vitamin D deficiency

 

Alternate names:
25 Hydroxy Vitamin D3, 25(OH) Vitamin D, Vit D
Laboratory:
Biochemistry
Test Code:
VITD
Specimen types:
Serum
Container types:
Serum tube with gelSerum tube with gel
Collection Instructions:
Transport specimen at Room Temperature to lab

Store and transport serum at 4 o C

Note: Vitamin D can be added on to the specimens up to 48 hours after the collection (at room temperature) or up to 7 days for specimen stored at 4 o C.

 

Minimum Adult Volume:
1 mL
Notes:

 

  Specimens for vitamin assays must be taken before dietary changes are implemented  

Please note: Total Vitamin D measures both Vitamin D2 and D3.

Frequency:
Daily
Result Turnaround Time:
5 hours
Reference Interval:

> 50 nmol/L

 

 Vitamin D levels >50 nmol/L at the end of winter are recommended. Level should be 10-20 nmol/L higher at the end of summer to allow for seasonal decrease.

 Vitamin D levels < 50 nmol/L indicate insufficiency. Levels <25 nmol/L indicate significant deficiency.

Sources: RCPA Position Statement May 2019, "Use and Interpretation of Vitamin D testing"

Nowson CA, McGrath JJ, Ebeling PR, et al. Vitamin D and health in adults in Australia and New Zealand: a position statement. Med J Aust 2012; 196: 686-7.

25 Hydroxy Vitamin D3 is the appropriate metabolite to asess in suspected deficiency states.

 

 

Occasionally 1,25 di-Hydroxy Vitamin D3 is indicated, but discussion with Chemical Pathologist is required prior to testing by an external provider.

 

 

Enquiries - Phone Core Laboratory Box Hill Hospital; 9895 3473

 

Laboratory Use Only

  

 

If EMR is not available, Pathology results are available from Auscare.  If you do not have an Auscare login,please complete attached form. https://pathology.easternhealth.org.au/handbook/downloads/Auscare%20access.pdf

Instruction on how to use Auscare - https://pathology.easternhealth.org.au/handbook/downloads/Auscare%20instructions.pdf

SPECIMEN COLLECTION MODULE (SCM) FAQs - CLICK HERE

CLICK HERE for a consent form to perform tests not covered by Medicare

TURN AROUND TIMES - When will my result be available?

CLICK HERE for Minimum Labelling Requirements

CLICK HERE for a printable Pathology request form

CLICK HERE for a Log sheet for recording Pathology results

"THE ART OF CCCCOLLECTION"

PLEASE CLICK HERE FOR THE PREFERRED ORDER OF DRAW

 

Pathology Handbook

Test & Collection Guide

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