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Select department: Select...AllAnatomical PathologyBiochemistryBlood BankCoagulationCytopathologyFlow CytometryHaematologyImmunologyMicrobiologyReferred TestSpecial HaemostasisSpecimen CollectionUnassigned
Recent changes for Adrenocorticotrophic Hormone
Consultation with Endocrinologist suggested
Collect in the morning between 0800 and 1000h.
1 x 4mL EDTA tube on ice.
Send to laboratory for immediate centrifugation. Spin, separate and freeze plasma. Transport frozen.
Collect 1 x 4mL EDTA tube on ice.
Send to laboratory for immediate centrifugation. Spin, separate and freeze plasma.
Recent changes for Aldosterone - blood
Referred Test
Biochemistry
Collection Requirements for both or either tests
Collect into 4 mL EDTA tubes at Room Temperature. Do not collect on ice
If both Renin and Aldosterone are requested together, they can be collected into the same EDTA tube
In the laboratory, spin and separate the plasma immediately. Plasma is stable at Room Temperature if analysis is performed on the same day
If the specimen arrives on the weekend or after hours, or if the specimen is referred from an external laboratory, freeze the plasma
EDTA plasma samples from external laboratories should be stored and transported frozen
Whole blood or plasma stored at 4 Deg C is not suitable
Add-on tests for Renin and Aldosterone should not be done on samples which have been previously collected or stored at 4 Deg C
If the specimen arrives on the weekend or after hours freeze the plasma
Austin Pathology
Recent changes for Alpha-1 Antitrypsin - Quantitation
St Vincents Hospital
Recent changes for Anti-Heart Antibodies
Pathwest Lab Med WA
Recent changes for Anti-Myocardial Antibodies
Recent changes for Aspergillus precipitins
Twice weekly
Weekly
Immunodiffusion for Aspergillus fumigatus antibodies in allergic Aspergillosis.
Test for Aspergillus fumigatus antibodies.
Monash Pathology
Test performed by Royal North Shore Hospital, NSW
Sent with ICPMR courier
Recent changes for Azathioprine Metabolite Testing
Place the samples and request slip in fridge (M43) in CSR. Do not enter in the computer.
Eastern Health Clinical School Level 2, 5 Arnold Street, Box Hill Vic 3128 Ph: 61 3 9091 8827 Fax: 61 3 9899 9137 E-mail: [email protected]
Recent changes for Bile Acids
Plain Tube with gel
Serum tube with gel
Fasting or Non Fasting sample.
Note: This test is ONLY performed on pregnant women.
Fasting.
Spin, separate ASAP.
Royal Childrens Hospital
Store and transport at 4 C.
Recent changes for Cadmium
Transport specimen at Room Temperature to lab Sample haemolysis may make this test unreportable
Send as whole blood
Recent changes for Caeruloplasmin
Recent changes for Cholesterol, Low Density Lipoprotein
LDL is not measured directly, but is calculated using the Friedwald formula:
(LDLC) = (total CHOL) - (HDLC) - (TG/2.2)
This formula cannot be used where TG > 4.5 mmol/L
LDL was previously calculated from triglyceride, cholesterol and HDL cholesterol using the Friedewald formula.
As from 22nd of February 2024, LDL is calculated using the Sampson equation. Friedewald knew that with increasing triglyceride levels, the calculated LDL-C was progressively falsely lower when compared with the gold standard technique and therefore Friedewald stated LDL-C should not be calculated when triglycerides are > 4.5 mmol/L. With much lower LDL-C levels seen in the statin era, an improved calculation was required, hence the Sampson equation.
Comparing both equations with the gold standard ultracentrifugation method, Sampson LDL-C offers similar or better accuracy than Friedewald LDL-C at any triglyceride level. Sampson LDL-C can also be calculated up to a triglyceride level of 9 mmol/L.
Friedewald: LDL-C = TC – HDL-C – Triglycerides/2.2
Sampson: LDL-C=TC/0.948−HDL-C/0.971−(TG/8.56+TG × non-HDL-C/2140 −TG2/16100)−9.44
The calculation for the Sampson equation is complicated but Friedewald LDL-C can be (easily) calculated from the available lipid results if required.
Recent changes for CMV Serology
IgG and IgM, IgG Avidity Index performed
IgG and IgM, IgG Avidity Index performed if IgM is positive
Microbiology
Recent changes for Creutzfeldt-Jakob Disease
Requests for Alzheimer's disease - CSF must be collected in a seperate blue capped PP tube, available from Pathology Specimen Reception (CSR).
DO NOT CENTRIFUGE SPECIMEN
This test requires authorisation by the Neurology Registrar or Neurology Consultant.
CSF Specimens are unsuitable for testing if macroscopically haemorrhagic, xanthochromic, have red blood cell counts < 500/uL, have white cell counts < 10/uL
CSF Specimens are unsuitable for testing if macroscopically haemorrhagic, xanthochromic. Cell count must have red blood cell counts < 500/uL, and white cell counts < 10/uL
In addition, please refer to Eastern Health Infection Control CJD procedure and contact Infection Control prior to performing the Lumbar Puncture
Test is run weekly. Samples received by 4 p.m. Friday will have the result issued by the end of the following week.
CSF : Tests for 14-3-3 CSF protein
At least 1 mL of CSF is required in a separate (4th), unopened tube.
Double bag specimen. Must be frozen and transported on dry ice.
Blood : Test for PRNP (Prion protein gene)
5 mLs in EDTA tube
Double bag specimen. Note: sample is no longer required to be sent frozen.
Recent changes for CSF BioFire Meningitis/Encephalitis Panel
This test required approval from the Clinical Microbiologist. Minimum 200uL/0.2mL of unspun CSF is required for this test.
CSF collected via lumbar punture only; bloodstained/xanthochromic samples are suitable
NOTE: Non-K1 E. coli serotypes may be present in a specimen and will not be detected by the BioFire ME Panel. NOTE: Non-encapsulated strains of Neisseria meningitidis are not detected by the BioFire ME Panel. NOTE: The BioFire ME Panel does not distinguish between latent and active CMV and HHV-6 infections. Detection of these viruses may indicate primary infection, secondary reactivation, or the presence of latent virus. Results should always be interpreted in conjunction with other clinical, laboratory, and epidemiological information. NOTE: Patients with a suspicion of cryptococcal meningitis and a negative cryptococcal PCR result, such as by the BioFire ME Panel, should be tested for CrAG.
Recent changes for D-Dimer
D Dimer, DDimer, Fibrin breakdown products
D Dimer, DDimer
Recent changes for EBV Serology
Recent changes for Erythrocyte Sedimentation Rate
1.5 mL
1 mL
0.5 mL
ESR tube
EDTAEDTA - Paediatric
Using the ESR tube :Transport specimen at Room Temperature to labSample haemolysis may make this test unreportable
Transport specimen at Room Temperature to labSample haemolysis may make this test unreportableSamples found to have clots cannot be tested
This tube consists of an inner vessel with an outer sleeve. The outer sleeve MUST remain in place as this is where sample identification (label or handwritten) will be placed.
Daily
If the ESR test is requested in conjunction with a FBE test request, a single EDTA tube may be shared. In these instances;
Minimum Adult Volume: 1.5 mL
Minimum Paediatric Volume: 1 mL
Recent changes for Faecal Calprotectin
For non-rebatable tests the out of pocket cost is $60
Sample must be a dedicated collection and be frozen at -20 upon recipept at the laboratory. Sample is stable for 3 days at 2-8 degrees.
For non-rebatable tests the out of pocket cost is $75
20 g
1 g
Samples can be sent frozen.
Contact
Monash University, Eastern Health Department of Gastroenterology IBD Gastroenterology Laboratory Level 1, 5 Arnold St, Box Hill Vic 3128 Ph: 03 9895 4632 Email: [email protected]
Link to test information and costs :
http://www.med.monash.edu.au/ehcs/services/metabolite/calprotectindrsinformation.html
CALPRO
Recent changes for Flavivirus Serology
Dengue fever serology, Kunjin serology, Murray Valley Encephalitis serology
Dengue fever serology, Kunjin serology, Murray Valley Encephalitis serology, Japanese B encephalitis
SDENG, OPCR, (SMVE, SJBE)
SDENG, SMVE, SJBE
Blood
BloodSerum
EDTA
EDTASerum tube with gel
OPCR
Procedure detects flaviviruses including Murray Valley encephalitis, dengue and kunjin viruses
PCR testing detects flaviviruses including Murray Valley encephalitis, dengue and kunjin viruses. Dengue specific serology tests for IgG, IgM, and NS1 antigen
Recent changes for Helicobacter pylori Breath Test
Arrangements can be made through Microbiology, Monash Medical Centre
Recent changes for Helicobacter pylori Serology
Victorian Infectious Diseases Reference Laboratory (V.I.D.R.L)
Contact VIDRL 9342 2649/9342 2650.
Recent changes for Hepatitis B Viral Load
Perfomed by Abbott Alinity M
Performed by Abbott Alinity M
Hepatitis B Quantitation
Hepatitis B Viral Load
Hepatitis B Viral Load, Hep B
HEPBVL
HBVL
Contact VIDRL 9342 9615
Recent changes for Herpes Virus PCR
0.3 mL
0.2 mL
Test performed is a multiplex PCR for the following viruses: Herpes simplex 1 virus (HSV-1); Herpes simplex 2 virus (HSV-2); Varicella zoster virus (VZV); Cytomegalovirus (CMV); Enterovirus (EV); human Adenovirus (hAdv). Swabs and CSF samples are performed in-house at Box Hill laboratory, other sample types are referred.
CSF minimum volume for testing: 200uL/0.2mL
CMV PCR, HSV, HSV I-II, HSV PCR, VZV and CMV NAD, VZV PCR
CMV PCR, HSV, HSV I-II, HSV PCR, VZV and CMV NAD, VZV PCR, Enterovirus PCR, Adenovirus PCR
HPCR
HPCR, (ENTPCR, ADEPCR)
Cerebrospinal FluidFluidTissueNasopharyngeal swabBlood
SwabCerebrospinal FluidTissueNasopharyngeal swabRespiratory samples
Respiratory samples
Tests included in Monash PCR - Herpes simplex 1 virus, Herpes simplex 2 virus, Varicella zoster virus, Cytomegalovirus, Enterovirus (swabs only), Adenovirus (not faeces)
Specimens tested at Monash-
Swabs (dry or in UTM), Sterile fluids, Respiratory samples, Urine.
Specimens anticoagulated with heparin are unsuitable as heparin inhibits PCR
For other specimens - refer to VIDRL. Tissue, CSF, saliva, serum, Plasma (ACD or EDTA),
If CSF Herpes and Enterovirus PCR -send to VIDRL.
Refer to Referred Molecular Test Requests WORK-MI-90
Specimens tested at Box Hill - Swabs (dry or in UTM), CSF
Specimens from other sites are referred to VIDRL for testing.
Contact Monash Pathology 9594 4532 or if no answer try Bacteriology 9594 4565 as there is someone rostered 24/7
Tested M, W, F
Routine runs are performed Monday and Thursday. Additional runs for urgent CSF samples are perfomed after discussion with the Clinical Microbiologist.
This test detects Herpes Simplex Virus 1(HSV-1), Herpes Simplex Virus 2 (HSV-2), Varicella zoster virus (VZV) and Cytomegalovirus (CMV).
Recent changes for Histopathology
During Business Hours: all Fresh Tissue for histopathology must be brought to the attention of an Anatomical Pathologist in the first instance. After Hours: All Fresh Tissue specimens (with the exception of “products of conception” – including placenta) the on-call Pathologist must be contacted for further instructions. Products of conception including placenta can be stored in the fridge until the next working day.
During Business Hours (0800-1630hours): ALL Fresh Tissue for histopathology must be brought to the attention of an Anatomical Pathologist in the first instance by the receipting/accepting laboratory. After Hours: All Fresh Tissue specimens (with the exception of “products of conception” – including placenta) the on-call Pathologist must be contacted for further instructions. Products of conception including placenta can be stored in the fridge until the next working day.
Recent changes for IgG Subclasses
Recent changes for Itraconazole
2 mL
5 mL
Lithium heparin no gel
Spin, separate and freeze plasma.
Spin and separate plasma.
Women's & Children's Hospital (Adelaide)
Alfred Hospital
Packed by certified personnal - transport frozen.
Results : 08-81617000, 08-81616732.
Monthly
Dose information should be provided and length of therapy must be >5 days.
Recent changes for Kleihauer stain
Kleihauer test
Kleihauer test, FMH (feto-maternal Haemorrhage)
KL
FMH
Recent changes for Lamotrigine
Lamictal
3 mL
Spin aliquot plasma. (Send either plasma or whole blood)
Spin & aliquot plasma
Australian Clinical Labs (formerly Healthscope)
Recent changes for Linezolid
Deliver to laboratory ASAP.
A separate unopened specimen is required for this test.
Sydpath Pathology, St Vincent's Hospital NSW
Send at 4 C.
As required
Results: Clinical Pharmacology on 02 8382 9184.
Recent changes for Malarial Parasites
Thick and Thin films
Malaria parasite investigation Thick and Thin films
Clinical history including details of overseas travel and prophylaxis required
An FBE is performed at the same time
ICT test (rapid whole blood immunochromatographic test) for the qualitative detection of Plasmodium falciparum and Plasmodium vivax antigens is performed in addition to microscopic examination.
Recent changes for Measles Serology
SMEA
MEA
Recent changes for Methotrexate
Send ASAP (within 24 hrs, 7 days a week). Contact reference laboratory prior to sending the sample.
Testing performed on an as needs basis.
Peter MacCallum Cancer Institute
Hand deliover samples to Biochemistry DO NOT load onto the 8100
Recent changes for Mumps Serology
SMUM
MUM
Recent changes for Muscle Biopsy
Note: This is an URGENT send out test.
Laboratory hours are 9am - 3pm, Monday - Friday.
Specimen must arrive at Alfred Pathology by 4pm.
Laboratory hours are 9am - 5pm, Monday - Friday.
Specimens need to be delivered to Leve4 Specimen Reception by 3pm as the specimen must arrive at Alfred Pathology by 4pm.
Place the specimen in an esky with ice cubes and dispatch immediately.
Place the specimen in an esky with ice cubes and dispatch immediately. Must arrive at Alfred by 4pm.
Recent changes for Neonatal Blood Group Serology
Neonatal Group & DAT Neonatal Group, Screen and DAT
BGN
BGN, GPHN
Cord blood or heelprick collection required
Must be accompanied by a Blood Bank request form with the declaration signed, timed and dated. The blood tube must also be signed, dated and timed; matching the request slip exactly.
Includes Neonatal ABO & Rh(D) Blood Group and Direct Coombs TestCord blood or heelprick collection required
Includes Neonatal ABO & Rh(D) Blood Group and Direct Coombs
Cord blood or heelprick collection required.
Recent changes for Plasma Free Haemoglobin
Plasma Free Heamoglobin
Plasma Free Haemoglobin
Samples must be hand delivered to the lab to avoid shaking, which will falsely raise the plasma free-haemoglobin. Normal operating plasma free-haemoglobin level is < 0.10 g/dL. This is performed 24-hourly whilst on ECMO (routinely taken with the daily blood samples) according to clinical need/ECMO clinician direction.
Samples are taken by passively filling a 10mL syringe carefully from the intra-arterial line access port. Do not connect to a negative pressure “vacutainer” for sampling. The plasma free-haemoglobin collection tube is filled from the syringe after removing the lid, gently injecting blood from the syringe and then replacing the lid. No needles are required.
Plasma
Serum
Recent changes for Procalcitonin
All PCT requests must be discussed with the Chemical Pathologist upon receipt in lab, regardless of time of day or day of week. If urgent/Taxi requiring is determined, result must be phoned to the clinician upon receipt - High Risk Result.
Recent changes for Q Fever Serology
ICPMR (Institute of Clinical Pathology and Medical Research)
Contact VIDRL 9342 2649 / 9342 2650
Test performed by Institute of Clinical Pathology and Medical Research (ICPMR), NSW
Recent changes for Quantiferon TB-Gold
QTFN, Quantiferon TB Gold
Quantiferon, TB Gold, QTFN
Recent changes for RAST
White top tube is the preferred sample.0.2ml of blood required for each allergen.
Blood collection: still can be done after skin allergy testing.
Omega-5-Gliadin, Alpha Gal and Cefaclor : serum send to Melbourne Pathology
IgG 96 Food Allergy test is not a RAST test It is performed at Australian Clinical Labs.This test is NOT rebatable and costs approx $260.
RAST for Omega-5-Gliadin to be sent to Alfred Pathology, Alpha Gal to be sent to Melbourne Pathology. Cefaclor and ARAh2 to be sent to Dorevitch Pathology
Omega-5-Gliadin: send to Melborne Pathology
Recent changes for Red Cell Antigen Genotyping
Minimum volume of 4 ml required - this specimen cannot be used for any other purpose.
A completed "ARCBS Red Cell Reference Form" must accompany each sample sent for analysis.
This form, which can be completed online and subsequently printed to be sent with the sample can be found by clicking here
Specimen will arrive in department at RT. Please keep at 4 degrees prior to transport.
Transport at 4 degrees
Recent changes for Renin - plasma
In the laboratory, spin and separate the plasma immediately. Plasma is stable at Room Temperature if analysis is performed on the same day.
Renin must be frozen at -80 C. Transport plasma at room temperature (Maroondah and Angliss) until it can be placed in -80 C Freezer.(Must be done within 3-4 hors).
Add-on tests for Renin and Aldosterone should not be done on samples which have been previously collected or stored at 4 C.
Renin will not be collected at Healesville Hospital and Yarra Ranges Health Collection Rooms.
Renin must be frozen at -80 C. Transport plasma at room temperature (Maroondah and Angliss) until it can be placed in -80 C Freezer.(Must be done within 3-4 hours).
Samples from Angliss and Maroondah must be collected within business hours to allow transport to Box Hill via courier.
Recent changes for Respiratory Virus PCR
The following specimens will be referred for testing-
Sputum, Bronchial Washings, Bronchoalveolar Lavages - VIDRL
Swab specimens for Respiratory Multiplex PCR will be referred to Monash Molecular Laboratory
As of Feb 2023 swab specimens for Respiratory Multiplex PCR will be tested in-house.
Nasopharyngeal swabNasopharyngeal Aspirate
Nasopharyngeal swabNTS - nose & throat swabNasopharyngeal Aspirate
SputumBronchial WashingsBroncho-alveolar Lavage
Viral Transport Media- NasopharyngealRespiratory aspiration pot
Viral Transport Media- NasopharyngealRespiratory aspiration potrespiratory PCR requirements
Please note these swabs are available from stores.
Search for: VIRAL TRANSPORT MEDIA - NASOPHARYNGEAL
Pathology do not keep a stock of these swabs. Emergency departments and wards should manage a supply of these swabs according to demand.
All tests for respiratory virus PCR are considered urgent and must be delivered to the laboratory ASAP.
ICU/Oncology patients testing negative for Flu A/B and RSV will be referred to Monash for respiratory virus panel. This includes Influenza A virus, Influenza B virus, Respiratory Syncitial Virus, Picornavirus (RV/EV), Parainfluenza viruses 1,2 and 3, Adenovirus, Human Metapneumovirus, Bordetella pertussis, Bordetella parapertussis, Mycoplasma pneumoniae, Legionella species, Chlamydiaceae.
Sputum, Bronchial Washings, Bronchoalveolar Lavages
Specimens for Respiratory Multiplex PCR will be referred to Monash Molecular Laboratory
This PCR will detect and identify: Influenza A virus, Influenza B virus and Respiratory syncytial virus (RSV).
Transport at room temperature to the Laboratory
Testing is performed at Box Hill, Angliss, and Maroondah.
Testing Lab
Testing hours
TAT (from receipt in lab)
All sites
Available 24/7
1 hr
Samples received after hours may have a longer TAT due to other urgent critical pathology testing occurring.
6-12 hrs routine
2 hours (rapid approval form required)
DO NOT send samples in the Pathology Chute
For Rapid testing from Emergency Department - swab must be accompanied by signed Rapid Test Authorisation Form.
DO NOT call the laboratory for results/enquiries. Inpatient tests will be prioritised and results available on EMR ASAP.
Sputum samples are referred to an external laboratory - results will be delayed.
Recent changes for Respiratory Virus PCR - Extended
Viral Transport Media - Nasopharyngeal
Viral Transport Media - Nasopharyngealrespiratory PCR requirementsViral Transport Media- Nasopharyngeal
NTS - nose & throat swabSwab
NTS - nose & throat swabRespiratory samples
Recent changes for Rickettsia spp. Screen Serology
Tests for Total Ab, IgM response to Spotted fever, Typhus and Scrub Typhus groups.
Tests for serology response to Spotted fever, Typhus and Scrub Typhus groups.
Recent changes for Ristocetin Co-Factor assay
Fortnightly
Recent changes for Soluscopes for sterility testing
Soluscope should be sent to laboratory in sterile container
Culture
not required
Not required
Sterile Jar
Recent changes for Stratify JCV
Samples are be aliquoted and frozen in the barcoded tube supplied in the JCV kit
Recent changes for Thiopurine Metabolites
Lithium heparin no gelEDTA
See Instructions
Collect 1 x 4 ml Lithium Heparin tube and
1 x 4ml EDTA tube.
FBE must be requested with this test as a RCC is required for calculation of results
DO NOT SPIN.
Keep at Room Temp.
2 x 4ml EDTA tube.
Collect 1 x 4 ml Lithium Heparin tube and 1 x 4ml EDTA tube.
TMETAB
Transport specimen at Room Temperature to lab
Collect 2x4ml EDTA blood. DO NOT SPIN
Keep at 4 deg C, do not freeze.
The metabolites are stable for upto 4 days at 4 degress.
Keep samples at Room Temperature.
Do Not Spin. Whole Blood required
Imuran is a trade name for azathioprine
The Thiopurine metabolites (6-TGN & 6-MMP) are used as a means of estimating the toxicity and efficacy of azathioprine treatment.
This test is performed by the Gastroenerology department. ( Phone No.9895 4632 )
The cost for the test is not covered by Medicare.
Eastern Health patients:6TGN 6MMP Thiopurine Metabolites Testing $75
All other patients: 6TGN 6MMP Thiopurine Metabolites Testing $145
Recent changes for Toxoplasma serology
STOX
TOX
Recent changes for Transthyretin
The serum must be aliquoted and frozen. Transthyretin is only stable for 3 days at 4-8C .
Recent changes for Typhoid
enteric fever, Typhoid fever
enteric fever, typhoid fever
Blood Culture
Blood Culture (Adult)Blood Culture (Paediatric)
Recent changes for Vitamin B1
Recent changes for Vitamin B6
Recent changes for von Willebrand Screen
Unassigned
Haematology
Recent changes for vWF Activty
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